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BackgroundIn the management of Peripheral Arterial Disease (PAD), the administration of anticoagulant or antiplatelet agents is imperative. The use of Dual Antiplatelet Therapy (DAPT) in conjunction with rivaroxaban has shown potential in mitigating adverse outcomes. Given the heterogeneity in the pathology of lower limb arteries, there is a compelling case for individualized treatment strategies.MethodsIn a single-center retrospective study on pharmacotherapy for peripheral artery disease, patients were treated with either aspirin combined with rivaroxaban or aspirin coupled with clopidogrel. The primary efficacy outcome encompassed a composite of increases in the Rutherford classification, acute limb ischemia, amputations due to vascular causes, target lesion revascularization, myocardial infarction, ischemic stroke, and cardiovascular death. The primary safety outcome was major bleeding, as defined by the Thrombolysis in Myocardial Infarction (TIMI) criteria; meanwhile, major bleeding as categorized by the International Society on Thrombosis and Haemostasis (ISTH) served as a secondary safety outcome. The study differentiated between two subgroups: patients with only above-the-knee and below-the-knee arterial lesions.ResultsFrom January 2016 to December 2021, 455 patients received either clopidogrel plus aspirin or rivaroxaban plus aspirin following endovascular treatment (EVT). The rivaroxaban group (n = 220) exhibited a lower incidence of primary efficacy outcomes [49.1% vs. 60.4%, hazard ratio (HR) 0.77, P = 0.006] but had more TIMI major bleeding events (5.9% vs. 2.1%, HR 2.6, P = 0.04). ISTH major bleeding events did not show a significant difference, though a higher percentage of rivaroxaban patients discontinued medication due to bleeding (10% vs. 4.7%, HR 2.2, P = 0.03). In the above-the-knee arterial disease subgroup, the rivaroxaban group demonstrated a lower incidence of primary efficacy outcomes (28.2% vs. 45.2%, HR 0.55, P = 0.02). In the below-the-knee arterial disease subgroup, no significant difference was observed in the occurrence of primary efficacy events between the two groups (58.7% vs. 64.8%, HR 0.76, P = 0.14).ConclusionRivaroxaban plus aspirin improved outcomes compared to DAPT in patients with lower extremity artery disease. Similar findings were observed in the above-the-knee artery lesion-only group. However, in the below-the-knee artery lesion-only group, rivaroxaban plus aspirin did not surpass DAPT in efficacy. Regarding safety, rivaroxaban plus aspirin exhibited higher bleeding risks and more frequent treatment discontinuation than aspirin combined with clopidogrel.

Non-judicious and indiscriminate use of veterinary drugs in animal husbandry may result in accumulation of residues in animal tissues, and consequently in food for human consumption. The abuse of veterinary drugs presents a potential risk to consumer health, especially if the residue level is higher than the health-based guidance value (HBGV) such as the acceptable daily intake (ADI). Contamination of drug residues in food also promotes the emergence of antimicrobial resistance (AMR) which poses a serious threat to public health globally. There has been limited information on the occurrence and dietary exposure to veterinary drug residues in Singapore to date. In this study, the occurrence of four classes of veterinary drugs, namely beta-agonists, coccidiostats, fluoroquinolones and macrolides, were determined using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in food widely consumed by Singapore residents. The magnitude of dietary exposure was assessed based on the consumption profile of Singapore population. Out of 216 food samples, 9.72 % were detected positive with veterinary drug residues, where majority of the positive samples were poultry and its derived products, followed by eggs and egg products. 7 veterinary drugs, specifically ciprofloxacin, enrofloxacin, clopidol, diclazuril, lasalocid, nicarbazin and tilmicosin, were detected in the samples, with clopidol and enrofloxacin being the most frequently detected drugs. Dietary exposure was evaluated using the estimated daily intake (EDI) of the detected drugs and benchmarked against the corresponding acceptable daily intake (ADI). All the %ADI values were far less than 100 in both the average and high consumer scenarios, indicating that the health risk associated with dietary exposure to these drugs in Singapore is low.

Abstract Background Mycobacterium haemophilum is a slow-growing non-chromogenic nontuberculous Mycobacterium species that can cause skin infection or arthritis in an immunocompromised population or in children. Primary infection of the healthy adult cornea is rare. The special requirements for culture make this pathogen difficult to diagnose. The study aims to report the clinical manifestation and treatment process of corneal infection and notify the awareness of M. Haemophilus keratitis among clinicians. This is the first case report of primary M. haemophilum infection in the cornea of healthy adults reported in the literature. Case presentation A 53-year-old healthy goldminer presented with left eye redness and a history of vision loss for four months. The patient was misdiagnosed with herpes simplex keratitis until M. haemophilum was detected using high-throughput sequencing. Penetrating keratoplasty was performed, and a large number of mycobacteria were detected by Ziehl-Neelsen staining of the infected tissue. Three months later, the patient developed conjunctival and eyelid skin infections that manifested as caseous necrosis of the conjunctiva and skin nodules. After excision and debridement of the conjunctival lesions and systemic antituberculosis drug treatment for 10 months, the patient was cured. Conclusion M. haemophilum could cause primary corneal infection in healthy adults, which is an infrequent or rare infection. Owing to the need for special bacterial culture conditions, conventional culture methods do not provide positive results. High-throughput sequencing can rapidly identify the presence of bacteria, which aids in early diagnosis and timely treatment. Prompt surgical intervention is an effective treatment option for severe keratitis. Long-term systemic antimicrobial therapy is crucial.

Jujube witches' broom (JWB) disease, referred to as 'Zaofeng' disease in Chinese, is associated with the JWB phytoplasma ('Candidatus Phytoplasma ziziphi') and causes heavy losses in the jujube industry. JWB disease has been recorded since the 1950s. Diseased trees have symptoms such as shoot proliferation and leaf-like flowers. The JWB phytoplasma is assigned to the 16SrV group, subgroup 16SrV-B, according to 16S rRNA gene sequence. The JWB phytoplasma is transmitted by leafhoppers and can infect a few plants other than jujube. Infection with phytoplasma affects biochemical and physiological process, altering the expression of genes encoding some transcription factors and functional genes, mainly involved in biotic stress response. The genome of JWB phytoplasma 'nky' strain has been sequenced and consists of 750,803 bp within one circular chromosome that encodes 694 protein-coding genes. The pathogenic mechanisms of a few JWB phytoplasma effectors have been investigated. The presence of JWB phytoplasma has been detected through symptoms observation, ELISA, DAPI staining and PCR, but new techniques, such as LAMP and CRISPR/Cas-12 based visual assay, have recently been developed. Some resistant jujube cultivars have been selected by infection screening. Although treatment with tetracycline antibiotics is effective, comprehensive control measures, including orchard management and sanitary measures, are needed for disease control. Further studies are needed in development of JWB phytoplasma culture method, expansion of genomic information, and phytoplasma effectors, resistance-related gene identification and resistant genotype developing.

Ethylene plays an important role in grape ripening, acting through ethylene response factors (ERFs) that are downstream of ethylene signaling pathways, yet little else is known about these molecular mechanisms. In our study, we demonstrate that ethylene treatment promotes grape ripening and that the transcription factor VvERF75 shows increased expression during grape ripening. VvERF75 was found to contain a conserved AP2/ERF domain, to be a member of the ERF IXa subfamily, and to localize to the nucleus and to have transactivation activity in yeast. Our in vitro assays showed that VvERF75 can positively regulate expression of pheophorbide a oxygenase (VvPAO1) and 1-aminocyclopropane-1-carboxylic acid synthase (VvACS5) by binding to DRE motifs in their promoters. Overexpression of VvERF75 in tomato increased ethylene production and resulted in dwarfed plants, early flowering and fruit ripening, increased SlPAO and SlACS transcript levels, and decreased chlorophyll content. In general, our study revealed that VvERF75 may regulate fruit ripening by promoting ethylene biosynthesis and chlorophyll degradation, a discovery that lays a foundation for deciphering the molecular mechanisms of grape ripening.

In irregular repetition slotted ALOHA (IR-SA) systems, a population of devices transmits their packets to an access point (AP) within a frame of slots. The AP decodes these packets by iterative processing between intra- and inter-slot successive interference cancellations. The average normalized offered traffic, as a performance metric, represents the number of packets transmitted per slot when the packet loss rate approaches zero. Such asymptotic types of traffic as the belief propagation (BP) threshold, the maximum a posteriori (MAP) threshold, and the converse bound of IR-SA systems have been analyzed over various channel models. However, over fading channels, the MAP threshold and the converse bound have not yet been investigated. This paper derives an MAP threshold and a converse bound of the systems over Rayleigh block fading channels. The derivations are based on two extrinsic information transfer (EXIT) curves, which are associated with two iterative density evolution equations to analyze the BP threshold of the IR-SA systems. First, since an open decoding tunnel exists in an EXIT chart, the sum of the two areas below two EXIT curves is smaller than the area of the entire domain. This provides the traffic’s converse bound, which is tight. Second, a coincidence of the BP EXIT and MAP EXIT curves makes it possible to derive the traffic’s MAP threshold. Third, a density evolution for a spatially-coupled scheme is formulated and gives a BP decoding threshold of the traffic. Numerical results show that the spatially-coupled scheme achieves a threshold saturation effect where the BP threshold approaches the MAP threshold.

In this work, a prescribed-time second-order sliding mode (SOSM) controller is constructed to address a class of nonlinear systems in the presence of asymmetric output constraints. To handle the asymmetric constraints, a frequently-used asymmetric barrier Lyapunov function is employed. Meanwhile, a time-varying scaling function is introduced to ensure the prescribed-time convergence of the closed-loop sliding mode system. On this basis, a prescribed-time SOSM controller is constructed by inserting the asymmetric BLF and time-varying scaling function into a modified power integrator technique. The rigorous theoretical analysis indicates that under the proposed prescribed-time SOSM control scheme, the overall system is prescribed-time stable without violating the asymmetric constraints. The distinguishing feature is that the convergence time of the overall system can be specified beforehand and does not depend on any system initial conditions. Finally, an academic simulation example is provided to demonstrate the effectiveness of the presented prescribed-time control scheme.

This paper investigates the issue of asynchronous $ H_{\infty} $ tracking control for nonlinear semi-Markovian jump systems (SMJSs) based on the T-S fuzzy model. Firstly, in order to improve the performance of network control systems (NCSs) and the efficiency of data transmission, this paper adopts a double quantization strategy which quantifies the input and output of the controllers. Secondly, for the purpose of reducing the burden of network communication, an adaptive event-triggered mechanism (AETM) is adopted. Thirdly, due to the influence of network-induce delay, the system mode information can not be transmitted to the controller synchronously, thus, a continuous-time hidden Markov model (HMM) is established to describe the asynchronous phenomenon between the system and the controller. Additionally, with the help of some improved Lyapunov-Krasovski (L-K) functions with fuzzy basis, some sufficient criteria are derived to co-guarantee the state stability and the $ H_{\infty} $ performance for the closed-loop tracking control system. Finally, a numerical example and a practical example are given to verify the effectiveness of designed mentality.

T-LAK-cell-originated protein kinase (TOPK), a novel member of the mitogen-activated protein kinase family, is considered an effective therapeutic target for skin inflammation. In this study, a series (A − D) of paeonol derivatives was designed and synthesised using a fragment growing approach, and their anti-inflammatory activities against lipopolysaccharide (LPS)-induced nitric oxide production in RAW264.7 cells were tested. Among them, compound B12 yielded the best results (IC50 = 2.14 μM) with low toxicity (IC50 > 50 µM). Preliminary mechanistic studies indicated that this compound could inhibit the TOPK-p38/JNK signalling pathway and phosphorylate downstream related proteins. A murine psoriasis-like skin inflammation model was used to determine its therapeutic effect.

Abstract Background The rapid growth of annual shoots is detrimental to peach production. While gibberellin (GA) promotes the rapid growth of peach shoots, there is limited information on the identity and expression profiles of GA-metabolism genes for this species. Results All six GA biosynthetic gene families were identified in the peach genome, and the expression profiles of these family members were determined in peach shoots. The upstream biosynthetic gene families have only one or two members (1 CPS, 2 KSs, and 1 KO), while the downstream gene families have multiple members (7 KAOs, 6 GA20oxs, and 5 GA3oxs). Between the two KS genes, PpKS1 showed a relatively high transcript level in shoots, while PpKS2 was undetectable. Among the seven KAO genes, PpKAO2 was highly expressed in shoots, while PpKAO1 and − 6 were weakly expressed. For the six GA20ox genes, both PpGA20ox1 and − 2 were expressed in shoots, but PpGA20ox1 levels were higher than PpGA20ox2. For the five GA3ox genes, only PpGA3ox1 was highly expressed in shoots. Among these biosynthesis genes, PpGA20ox1 and PpGA3ox1 showed a gradual decrease in transcript level along shoots from top to bottom, and a similar trend was observed in bioactive GA1 and GA4 distribution. Among the GA-deactivation genes, PpGA2ox6 was highly expressed in peach shoots. PpGA2ox1 and − 5 transcripts were relatively lower and showed a similar pattern to PpGA20ox1 and PpGA3ox1 in peach shoots. Overexpression of PpGA20ox1, − 2, or PpGA2ox6 in Arabidopsis or tobacco promoted or depressed the plant growth, respectively, while PpGA3ox1 did not affect plant height. Transient expression of PpGA20ox1 in peach leaves significantly increased bioactive GA1 content. Conclusions Our results suggest that PpGA20ox and PpGA2ox expression are closely associated with the distribution of active GA1 and GA4 in peach annual shoots. Our research lays a foundation for future studies into ways to effectively repress the rapid growth of peach shoot.

Abstract The seasonal rainfall regime is a key factor control on local ecological and social processes and is commonly thought to be stable under long‐term climate changes. Here we present a unique high‐resolution rainfall record from the Thai‐Malay Peninsula, combined with a state‐of‐the‐art transient climate simulation, demonstrating a fundamental rainfall regime shift from summer to winter during the Holocene. Transient model simulation and new sensitivity experiments further reveal that westward migration of the boundary between summer and winter rainfall regimes results in a summer to winter rainfall regime shift forced by distinct changes in summer and winter monsoons. Our findings suggest that the seasonal rainfall regime could be unstable under climate change around the boundaries of rainfall regimes in the tropics and possibly worldwide, which might be more critical for shaping both past and future ecological environments.

ObjectivesCarbapenem-resistant Klebsiella pneumoniae (CRKP) have been extensively disseminated worldwide, resulting in increased mortality. We performed a retrospective analysis of the epidemiology and risk factors for the outcome of CRKP infection in a general teaching hospital in China.MethodsA molecular and clinical study was conducted for 98 CRKP in a tertiary hospital from January 2013 to December 2016. Carbapenemase gene detection, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) were performed. Logistic regression was also used to identify the risk factors associated with 30-day mortality.ResultsThe production of KPC carbapenemase was the main resistant mechanism, and KPC carbapenemase increased annually with a significant difference. However, the molecular outcome revealed the dominance and diversity in CRKP with 24 sequence types (STs) and 59 PFGE types (PTs). The ST11 CRKP strains, which showed a significant increasing trend year by year, were documented as predominant in our study. Additionally, the predominant ST11 CRKP corresponding to PT10 and PT15 continued to exhibit their characteristic patterns. Importantly, the newly identified PT09 and PT16 strains, corresponding to the ST11 lineage, were only discovered in 2016. Meanwhile, factors affecting 30-day mortality and ST11 proportionality with CRKP infection were assessed, and ST11, appropriate empirical treatment, and hospital stays were found to be independently associated with 30-day mortality.ConclusionThe ST11 CRKP strains played a dominant role in the process; however, the homology of these strains was polymorphic, and the advantage clusters were subject to changes through evolution. Furthermore, in addition to appropriate empirical treatment and hospital stays, ST11 CRKP was independently associated with 30-day mortality. To the best of our knowledge, this association was reported for the first time.

PurposeTo compare the surgical outcomes of combined penetrating keratoplasty (PK) and cataract surgery with those of sequential surgery (cataract surgery after PK) for herpes simplex keratitis (HSK).MethodsThe medical records of consecutive patients diagnosed with HSK who underwent combined or sequential PK and cataract surgery in active and stable stages between June 2015 and June 2022 were reviewed retrospectively. Complications, graft survival, endothelial cell density (ECD), and final BCVA were compared and analyzed between both surgical methods in each stage.ResultsA total of 171 eyes of 171 patients were enrolled, including active stage (69 combined, 46 sequential) and stable stage (34 combined, 22 sequential). The average follow up was 24.2 ± 15.8 months (range, 3 months – 48 months). The final BCVA had obvious improvement and the postoperative ECD was not different in combined and sequential groups of each stage. In sequential group of active stage, 66.7% of persistent epithelial defects and 50% of HSK recurrence occurred within 3 months after cataract surgery; nevertheless, compared to that in sequential group, capsular rupture (p = 0.021), persistent epithelial defects (p = 0.027), and HSK recurrence (p = 0.035) occurred more frequently in combined group, leading to a lower graft survival rate (p = 0.045); at the last visit, 46.4 and 67.4% of grafts remained clear in combined and sequential groups, respectively. By contrary, 82.4 and 50.0% of grafts remained clear in stable stages of combined and sequential groups at the last visit, respectively, and a higher graft survival rate was observed in combined group (p = 0.030).ConclusionAlthough the postoperative ECD is not different between two surgical groups in each stage, sequential surgery in active stage of HSK seems to have advantages in less complications and higher graft survival rate, whereas combined surgery in stable stage has a better outcome than that in sequential surgery.

Improving the efficiency of the GM cryocoolers is of great importance for energy saving and CO2 emission reduction due to the large amount of cryocoolers installed in the emerging fields of semiconductor manufacture and High Temperature Superconductors (HTS) cooling. Previous studies mainly focused on the losses analysis and optimization on the part of cold head, but the details of losses distribution in the parts of compressor and rotary valve were seldom carried out. In this paper, a numerical model of a single stage GM cryocooler including compressor, rotary valve and expander is built, and the feasibility of the model is verified by the experimental results. The losses characteristics of the whole cryocooler are studied based on the exergy analysis method with the help of the numerical model. The results indicate that the main losses are occurred in compressor and rotary valve, the value of exergy loss in compressor decrease with the cooling temperature, and accounts for more than 60% at all cooling temperature. The loss in rotary valve accounts for about 20% of the input electric power, and it does not significantly vary at different cooling temperatures. Pressure drop dominates the loss in the compressor and rotary valve. The insufficient heat exchange between the working gas and regenerative material is the main loss in regenerator, and the losses in regenerator increase significantly with the decrease of cooling temperature when the compressor and rotary valve are fixed. This study provides useful guides for the optimization of GM-type cryocoolers.

Interplanetary scintillation (IPS) refers to random fluctuations in radio intensity of distant small-diameter celestial object, over time periods of the order of 1 s. The scattering and scintillation of emergent radio waves are ascribed to turbulent density irregularities transported by the ubiquitous solar wind streams. The spatial correlation length of density irregularities and the Fresnel radius of radio diffraction are two key parameters in determining the scintillation pattern. Such a scintillation pattern can be measured and correlated between multi-station radio telescopes on the Earth. Using the “phase-changing screen” scenario based on the Born approximation, the bulk-flow speed and turbulent spectrum of the solar wind streams can be extracted from the single-station power spectra fitting and the multi-station cross-correlation analysis. Moreover, a numerical computer-assisted tomography (CAT) model, iteratively fit to a large number of IPS measurements over one Carrington rotation, can be used to reconstruct the global velocity and density structures in the inner heliosphere for the purpose of space weather modelling and prediction. In this review, we interpret the underlying physics governing the IPS phenomenon caused by the solar wind turbulence, describe the power spectrum and cross correlation of IPS signals, highlight the space weather application of IPS-CAT models, and emphasize the significant benefits from international cooperation within the Worldwide IPS Stations (WIPSS) network.

Expansins (EXPs) loosen plant cell walls and are involved in diverse developmental processes through modifying cell-walls; however, little is known about the role of PpEXPs in peach fruit. In this study, 26 PpEXP genes were identified in the peach genome and grouped into four subfamilies, with 20 PpEXPAs, three PpEXPBs, one PpEXPLA and two PpEXPLBs. The 26 PpEXPs were mapped on eight chromosomes. The primary mode of gene duplication of the PpEXPs was dispersed gene duplication (DSD, 50%). Notably, cis-elements involved in light responsiveness and MeJA-responsiveness were detected in the promoter regions of all PpEXPs, while ethylene responsive elements were observed in 12 PpEXPs. Transcript profiling of PpEXPs in the peach fruit varieties of MF (melting), NMF (non-melting) and SH (stony hard) at different stages showed that PpEXPs displayed distinct expression patterns. Among the 26 PpEXPs, 15 PpEXPs were expressed in the fruit. Combining the expressing patterns of PpEXPs in fruits with different flesh textures, PpEXPA7, PpEXPA13 and PpEXPA15 were selected as candidate genes, as they were highly consistent with the patterns of previous reported key genes (PpPGM, PpPGF and PpYUC11) in regard to peach fruit texture. The genes with different expression patterns between MF and NMF were divided into 16 modules, of which one module, with pink and midnightblue, negatively correlated with the phenotype of fruit firmness and was identified as PpEXPA1 and PpEXPA7, while the other module was identified as PpERF in the pink module, which might potentially effect fruit texture development by regulating PpEXPs. These results provide a foundation for the functional characterization of PpEXPs in peach.

A total diet study is often used to evaluate a population’s baseline dietary exposure to chemical hazards from across the diet. In 2021–2023, Singapore carried out a TDS, and this article presents an overview of the study design and methodological selections in Singapore’s TDS, as well as its relevance to ensuring food safety. A food consumption survey was conducted on Singapore citizens and permanent residents, where food consumption patterns of the Singapore population were identified. The selection of chemical hazards and foods for inclusion in Singapore’s TDS, as well as principal considerations on sampling, food preparation, and analytical testing are discussed. Commonly consumed foods by the Singapore population in food categories such as grain and grain-based products, meat and meat products, fish and seafood, vegetables, fruits, milk and dairy products were included in this study, and mean concentrations of chemicals tested in each food category were reported, with food categories possessing higher levels identified. Future work will include dietary exposure assessments for the population and analysis of the contributions by food and cooking method.

The J-TEXT capability is enhanced compared to two years ago with several upgrades of its diagnostics and the increase of electron cyclotron resonance heating (ECRH) power to 1 MW. With the application of electron cyclotron wave (ECW), the ECW assisted plasma startup is achieved; the tearing mode is suppressed; the toroidal injection of 300 kW ECW drives around 24 kA current; fast electrons are generated with toroidal injected ECW and the runaway current conversion efficiency increases with ECRH power. The mode coupling between 2/1 and 3/1 modes are extensively studied. The coupled 2/1 and 3/1 modes usually lead to major disruption. Their coupling can be either suppressed or avoided by external resonant magnetic perturbation fields and hence avoids the major disruption. It is also found that the 2/1 threshold of external field is significantly reduced by a pre-excited 3/1 mode, which can be either a locked island or an external kink mode. The disruption control is studied by developing prediction methods capable of cross tokamak application and by new mitigation methods, such as the biased electrode or electromagnetic pellet injector. The high-density operation and related disruptions are studied from various aspects. Approaching the density limit, the collapse of the edge shear layer is observed and such collapse can be prevented by applying edge biasing, leading to an increased density limit. The density limit is also observed to increase, if the plasma is operated in the poloidal divertor configuration or the plasma purity is increased by increasing the pre-filled gas pressure or ECRH power during the start-up phase.

Water molecules at solid–liquid interfaces play a pivotal role in governing interfacial phenomena that underpin electrochemical and catalytic processes. The organization and behavior of these interfacial water molecules can significantly influence the solvation of ions, the adsorption of reactants, and the kinetics of electrochemical reactions. The stepped structure of Pt surfaces can alter the properties of the interfacial water, thereby modulating the interfacial environment and the resulting surface reactivity. Revealing the in situ details of water structures at these stepped Pt/water interfaces is crucial for understanding the fundamental mechanisms that drive diverse applications in energy conversion and material science. In this work, we have developed a machine learning potential for the Pt(211)/water interface and performed machine learning molecular dynamics simulations. Our findings reveal distinct types of chemisorbed and physisorbed water molecules within the adsorbed layer. Importantly, we identified three unique water pairs that were not observed in the basal plane/water interfaces, which may serve as key precursors for water dissociation. These interfacial water structures contribute to the anisotropic dynamics of the adsorbed water layer. Our study provides molecular-level insights into the anisotropic nature of water behavior at stepped Pt/water interfaces, which can influence the reorientation and distribution of intermediates, molecules, and ions—crucial aspects for understanding electrochemical and catalytic processes.

Abstract Distributed scheduling is seldom investigated in hybrid flow shops. In this study, distributed two-stage hybrid flow shop scheduling problem (DTHFSP) with sequence-dependent setup times is considered. A collaborative variable neighborhood search (CVNS) is proposed to simultaneously minimize total tardiness and makespan. DTHFSP is simplified by incorporating factory assignment into machine assignment of a prefixed stage, and its solution is newly represented with a machine assignment string and a scheduling string. CVNS consists of two cooperated variable neighborhood search (VNS) algorithms, and neighborhood structures and global search have collaborated in each VNS. Eight neighborhood structures and two global search operators are defined to produce new solutions. The current solution is periodically replaced with a member of the archive farthest from it. Experiments are conducted , and the computational results validate that CVNS has good advantages over the considered DTHFSP.

The study reconstructed Holocene seasonal temperatures using 1,310 pollen records covering the Northern Hemisphere landmass, and show that both summer and winter temperatures peaked at ~7 ka BP, but with significant spatial variability.

Introduction: Pulmonary hypertension secondary to left heart disease (PH-LHD) is a common and fatal disease. However, no effective therapeutic targets have been identified. Objectives: Here, we set out to illustrate the functional role and underlying mechanisms of fatty acid-binding protein 5 (FABP5) in PH-LHD development. Methods: We performed a systematic analysis of datasets GSE84704 and GSE16624 to identify differentially expressed genes and then constructed protein-protein interaction network for significant modules. Potential target genes in the modules were validated by RT-qPCR and western blot in a PH-LHD mouse model. PH-LHD or sham mice were treated with FABP5 antagonist SBFI-26 or DMSO for 28 days. The role of FABP5 on cardiac function was determined by echocardiography, its impact on pulmonary vascular remodelling were evaluated with right heart catheter, histological analysis and western blot. In vitro, primary pulmonary adventitial fibroblasts were used to investigate the pro-fibrotic mechanisms involving in FABP5. Results: FABP5 was the only one dramatically upregulated along with increased protein expression in the established PH-LHD mouse model. Inhibition of FABP5 by SBFI-26 injection abrogated pulmonary artery remodelling in PH-LHD and improved cardiac function. In vitro, SBFI-26 or FABP5 siRNA blunted the TGF-β1-induced fibrotic response in cultured pulmonary adventitial fibroblasts. Mechanistically, FABP5 knockdown inhibited GSK3β phosphorylation and increased β-catenin phosphorylation. The wnt/β-catenin agonist SKL2001 diminished the antifibrotic effect of FABP5 knockdown on pulmonary adventitial fibroblasts under TGF-β1 stimulation. Conclusion: FABP5 is an important mediator of pulmonary artery remodelling and a potential therapeutic target for PH-LHD.

Abstract Background Lignocellulose is an important raw material for biomass-to-energy conversion, and it exhibits a complex but inefficient degradation mechanism. Microbial degradation is promising due to its environmental adaptability and biochemical versatility, but the pathways used by microbes for lignin degradation have not been fully studied. Degradation intermediates and complex metabolic pathways require more study. Results A novel actinomycete DF3-3, with the potential for lignin degradation, was screened and isolated. After morphological and molecular identification, DF3-3 was determined to be Streptomyces thermocarboxydus. The degradation of alkali lignin reached 31% within 15 days. Manganese peroxidase and laccase demonstrated their greatest activity levels, 1821.66 UL−1 and 1265.58 UL−1, respectively, on the sixth day. The highest lignin peroxidase activity was 480.33 UL−1 on the fourth day. A total of 19 lignin degradation intermediates were identified by gas chromatography–mass spectrometry (GC–MS), including 9 aromatic compounds. Genome sequencing and annotation identified 107 lignin-degrading enzyme-coding genes containing three core enzymatic systems for lignin depolymerization: laccases, peroxidases and manganese peroxidase. In total, 7 lignin metabolic pathways were predicted. Conclusions Streptomyces thermocarboxydus strain DF3-3 has good lignin degradation ability. Degradation products and genomics analyses of DF3-3 show that it has a relatively complete lignin degradation pathway, including the β-ketoadipate pathway and peripheral reactions, gentisate pathway, anthranilate pathway, homogentisic pathway, and catabolic pathway for resorcinol. Two other pathways, the phenylacetate–CoA pathway and the 2,3-dihydroxyphenylpropionic acid pathway, are predicted based on genome data alone. This study provides the basis for future characterization of potential biotransformation enzyme systems for biomass energy conversion.

Measurement(s) whole genome sequencing Technology Type(s) Illumina Sequencing • Oxford Nanopore Sequencing

IntroductionVitamin D has been known to be associated with asthma, particularly in children, while the evidence among adults is limited and inconclusive. This study aimed to investigate the association between serum, vitamin D concentrations, and the incidence of adult-onset asthma and also the modified effect caused by sleep patterns and genetic risks.MethodsA prospective cohort study with 307,872 participants aged between 37 and 73 years was conducted based on the UK Biobank, with a median follow-up of 12 years. The Cox proportional hazard model was applied to evaluate the association between vitamin D status and incident adult-onset asthma, and the modified effect was investigated by conducting stratified analysis according to sleep pattern score and genetic risk score, and subgroup analyses were performed by sex, age, BMI, and smoking status as well.ResultsIndividuals with optimal vitamin D concentration were associated with 11.1% reduced risk of incident asthma compared to those participants with deficient vitamin D (HR = 0.889; 95% CI: 0.820–0.964; p = 0.005). Moreover, stratification analysis demonstrated that the protective effect of vitamin D on asthma risk was modified by sleep patterns or genetic susceptibility, with the strongest protective effect being observed in the subpopulation with a moderate sleep pattern (HR = 0.883; 95% CI: 0.797–0.977; p = 0.016) and a moderate genetic risk (HR = 0.817; 95% CI: 0.711–0.938; p = 0.004). In subgroup analyses, the protective effect of optimal vitamin D levels was only significant among men, individuals younger than 60 years of age, overweight individuals, and current or previous smokers.ConclusionIncreased serum vitamin D levels were associated with a lower risk of incident adult-onset asthma, and this association was modified by sleep patterns and genetic predisposition to some extent.

BackgroundIBSP is a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family that plays a vital role in bone formation, renewal and repair. Emerging evidence revealed that IBSP participated in the tumorigenesis and progression in some cancers. However, its significance in tumour prognosis and immunotherapy is still unknown.MethodsIn the current study, we studied the role of IBSP in tumorigenesis, tumor diagnosis, genomic heterogeneity, methylation modifications, immune infiltration, and therapy response in pan-cancer. In addition, we constructed a risk score model to assessed the prognostic classification efficiency of IBSP using the co-expression genes of IBSP in osteosarcoma (OS), and analyzed the expression and role of IBSP in OS through a series of assays in vitro.ResultsIBSP was upregulated in various cancers compared to the paired normal tissues, and it was strongly correlated with the prognosis, pathological stage, diagnostic accuracy, genomic heterogeneity, methylation modification, immune infiltration, immune and checkpoint. Moreover, the predictive model we established in combination with the clinical characteristics of OS patients showed high survival predictive power in these individuals. The assays in vitro showed that IBSP promoted the proliferation, migration and invasion of OS cells, which further confirmed IBSP’s role in cancers.ConclusionsOur research revealed the multifunctionality of IBSP in the tumorigenesis, progression and therapy in various cancers, which demonstrated that IBSP may serve as a potential prognostic biomarker and a novel immunotherapy target in pan-cancer.

ObjectiveTo assess the effect of plaque and luminal morphologies in balloon angioplasty of femoropopliteal lesions using intravascular ultrasound (IVUS).MethodsThis retrospective, observational study analyzed 836 cross-sectional images using IVUS, from 35 femoropopliteal arteries of patients who underwent endovascular treatment between September 2020 and February 2022. Pre- and post-balloon angioplasty images were matched per 5 mm. Post-balloon angioplasty images were grouped into successful (n = 345) and unsuccessful (n = 491) groups. Plaque and luminal morphologies (such as severity of calcification, vascular remodeling, and plaque eccentricity) were extracted before the balloon angioplasty procedure to identify the predictors of unsuccessful balloon angioplasty. Additionally, 103 images with severe dissection were analyzed using IVUS and angiography.ResultsIn univariate analyses, the predictive factors for unsuccessful balloon angioplasty were vascular remodeling (p

Background: Numerous studies have explored the association of air pollution with asthma but have yielded conflicting results. The exact role of air pollution in the incidence of adult-onset asthma and whether this effect is modified by genetic risk, lifestyle, or their interaction remain uncertain. Methods: We conducted a prospective cohort study on 298,738 participants (aged 37–73 years) registered in the UK Biobank. Cox proportional hazard models were used to evaluate the association of air pollution, including particulate matter (PM2.5, PMcoarse, and PM10), nitrogen dioxide (NO2), and nitrogen oxides (NOx), with asthma incidence. We constructed genetic risk and lifestyle scores, assessed whether the impact of air pollution on adult-onset asthma risk was modified by genetic susceptibility or lifestyle factors, and evaluated the identified interactions. Results: We found that each interquartile range increase in annual concentrations of PM2.5, NO2, and NOx was related to 1.04 (95% confidence interval [CI]: 1.01, 1.08), 1.04 (95% CI: 1.00, 1.08), and 1.03 (95% CI: 1.00, 1.06) times the risk of adult-onset asthma, respectively. The size of the effect of air pollution was greater among subpopulations with low genetic risk or unfavorable lifestyles. We also identified an additive interaction effect of air pollution with lifestyle factors, but not with genetic risk, on the risk of adult-onset asthma. Conclusion: Our analyses show that air pollution increases the risk of adult-onset asthma, but that the size of the effect is modified by lifestyle and genetic risk. These findings emphasize the need for integrated interventions for environmental pollution by the government as well as adherence to healthy lifestyles to prevent adult-onset asthma.

Salt marshes, which commonly exist on the upper tidal flat, provide a natural barrier against sea level rise and coastal storm. The extremely shallow water stages (water depth< 0.2 m), including the initial stage of flood tides and the last stage of ebb tides, can induce a significant impact on sediment dynamics of saltmarshes and associated tidal flats, despite lasting for only a short time (around 10 min), which has been less studied. In this study, two parallel field sites were established to quantify erosion-accretion processes and morphological changes during extremely shallow water stages in salt marshes within Doulonggang tidal flat along the Jiangsu coast. Our results revealed that obvious accretion occurred during extremely shallow water stages, with a total deposition amount of +33.8 mm in vegetated areas and +20.8 mm in unvegetated areas. In contrast, erosion dominated during deep water stages, with a total erosion amount of -22.3 mm at the vegetated site and -32.7 mm at the unvegetated site. The magnitude of bed-level change during extremely shallow water stages was 7~8 times greater than that during deep water stages, even though the duration of extremely shallow water stages was only about 14~15% of the entire tidal cycle. Furthermore, strong winds significantly impacted deposition during extremely shallow water stages compared to calm weather. During the strong wind period, the average bed level change rate reached +0.15 mm/min and +0.12 mm/min in the vegetated and unvegetated areas, respectively. This is significantly higher than the +0.05 mm/min and +0.01 mm/min during the calm weather period. These results reveal that extremely shallow water stages have substantial impacts on sedimentary processes, which are vital for the maintenance of tidal flat systems.

Previous studies have found that subjective well-being is associated with social trust, self-compassion, and social empathy. Based on online fieldwork with 662 first-generation college students (54.7% male) in China, this study aimed to investigate the serial mediation effects of self-compassion and social empathy on the relationship between social trust and subjective well-being. The results showed that subjective well-being was significantly positively correlated with social trust, trust in people, self-compassion, and social empathy. Both self-compassion and social empathy partially mediated the relationship between social trust and subjective well-being, and fully mediated the association between trust in people and subjective well-being. We used a serial mediation model to estimate the effect of general social trust, including trust in people, on subjective well-being. The findings that self-compassion and social empathy mediated the relationship between trusting attitudes toward society, especially people, and subjective well-being expand the literature on social trust and the mechanism of social trust on subjective well-being. The results also highlight the significance of improving mental health education and intervention among first-generation college students in China.

Abstract Background Previous studies have indicated that pain‐related diseases can result in significant functional alterations in the brain. However, differences in spontaneous brain activity in toothache (TA) patients remain unclear. Objective To investigate altered spontaneous brain activity in patients with TA and its underlying mechanisms using the resting‐state functional magnetic resonance imaging–fractional amplitude of low‐frequency fluctuation (rsfMRI–fALFF) technique. Methods Twelve patients with TA and 12 non‐toothache controls (NTCs) (matched for sex, age, and level of education) were enrolled. Spontaneous cerebral activity variations were investigated using the rsfMRI–fALFF technique in all individuals. The mean fALFF values of the TA patients and NTCs were classified using receiver operating characteristic (ROC) curves. The correlations between fALFF signals of distinct brain regions and clinical manifestations of TA patients were evaluated using Pearson's correlation analysis. Results TA patients showed lower fALFF values in the left superior frontal gyrus, medial; right superior frontal gyrus, dorsolateral; and left median cingulate and paracingulate gyri (LDCG) than the NTCs. Moreover, ROC curve analysis indicated that the area under the curve of each cerebral region studied had high accuracy. Besides, in the TA group, the visual analog scale score was negatively correlated with fALFF signal values of the LDCG (r = .962, p

Solid–liquid interfaces are ubiquitous in natural and technological processes, but their imaging at the atomic scale has been challenging. The authors, using liquid-phase transmission electron microscopy, identify a quasi-liquid phase and the mass transport between the surface of In and Sn nanocrystals and an aqueous solution.

Abstract Background Ampullary adenocarcinoma (AAC) arises from the ampulla of Vater where the pancreatic duct and bile duct join and empty into the duodenum. It can be classified into intestinal and pancreatobiliary types based on histopathology or immunohistochemistry. However, there are no biomarkers for further classification of pancreatobiliary-type AAC which has important implications for its treatment. We aimed to identify the tumor origin of pancreatobiliary-type AAC by systematically analyzing whole-slide images (WSIs), survival data, and genome sequencing data collected from multiple centers. Methods This study involved three experiments. First, we extracted quantitative and highly interpretable features from the tumor region in WSIs and constructed a histologic classifier to differentiate between pancreatic adenocarcinoma (PAC) and cholangiocarcinoma. The histologic classifier was then applied to patients with pancreatobiliary-type AAC to infer the tumor origin. Secondly, we compared the overall survival of patients with pancreatobiliary-type AAC stratified by the adjuvant chemotherapy regimens designed for PAC or cholangiocarcinoma. Finally, we compared the mutation landscape of pancreatobiliary-type AAC with those of PAC and cholangiocarcinoma. Results The histologic classifier accurately classified PAC and cholangiocarcinoma in both the internal and external validation sets (AUC > 0.99). All pancreatobiliary-type AACs (n = 45) were classified as PAC. The patients with pancreatobiliary-type AAC receiving regimens designed for PAC showed more favorable overall survival than those receiving regimens designed for cholangiocarcinoma in a multivariable Cox regression (hazard ratio = 7.24, 95% confidence interval: 1.28–40.78, P = 0.025). The results of mutation analysis showed that the mutation landscape of AAC was very similar to that of PAC but distinct from that of cholangiocarcinoma. Conclusions This multi-center study provides compelling evidence that pancreatobiliary-type AAC resembles PAC instead of cholangiocarcinoma in different aspects, which can guide the treatment selection and clinical trials planning for pancreatobiliary-type AAC.

ABSTRACT: Objectives: Carbapenem-resistant Klebsiella pneumoniae (CRKP) is increasingly reported worldwide and has posed a serious challenge for public health. Here we report the complete genome sequence of a multidrug-resistant (MDR) K. pneumoniae carrying one blaNDM-1 and two copies of blaKPC-2 genes isolated from a cerebrospinal fluid specimen in China. Methods: The minimal inhibitory concentrations (MICs) of 26 antimicrobial agents against K. pneumoniae strain KP46 were measured. The complete genome sequence of KP46 was determined using Illumina and Nanopore platforms. The derived short and long reads were assembled using Unicycler. Multilocus sequence typing (MLST), antimicrobial resistance genes, virulence genes, and plasmid replicons were predicted in silico using the BacWGSTdb server. The phylogenetic relationship between KP46 and 454 ST15 K. pneumoniae strains obtained from NCBI GenBank database was analysed based on a core genome MLST (cgMLST) strategy. Results: K. pneumoniae strain KP46 was resistant to all antimicrobial agents tested, except for tigecycline, colistin, cefiderocol, and fosfomycin. The genome sequence of KP46 belonged to sequence type 15 (ST15), which contained seven circularized contigs comprising 5 674 521 bp, including one chromosome and six plasmids. Serval antimicrobial resistance genes were identified, including a blaNDM-1 gene located in a 53 096 bp IncX3 plasmid, and two copies of blaKPC-2 gene located both in a 103 807 bp IncX6 and an 88 164 bp IncFII plasmid, respectively. The most closely related strain was another ST15 strain also isolated from China with five cgMLST loci differences. Conclusion: We reported the first complete genome sequence of a K. pneumoniae ST15 clinical isolate coharbouring blaNDM-1 and two copies of blaKPC-2 in China. This study will provide insight into the antimicrobial resistance mechanisms and phylogeny of carbapenem-resistant ST15 K. pneumoniae.

The flattening behavior of in-flight particles during plasma spraying is a highly intricate process affected by numerous factors. Therefore, in this work, in-flight particles (spherical NiCrBSi powder) were collected with the water quenching process, and the morphology and composition differences between the original powder particles and the melted in-flight particles were observed using scanning electron microscopy (SEM). The particle size in various states was recorded and calculated. The internal structure of the particles was analyzed to elucidate their morphology and compositions under different flow rates of primary gas (FRPG). A coating with 1.25% porosity and a hardness of 767 HV0.5 was achieved at the FRPG of 80 L/min. X-ray diffraction (XRD) and transmission electron microscopy (TEM) were used to characterize the microstructures of the obtained coatings. It was found that the as-sprayed coating had a certain amount of the amorphous phase. A noteworthy correlation was also established, wherein a decreased distance from the substrate, augmented spraying passes, and reduced amorphous phase content were interrelated. Concurrently, a noticeable influence of the tamping effect exerted by the in-flight particles on the coating was observed.

This study investigated the prevalence and occurrence of 3-monochloropropanediol esters (3-MCPDEs) and glycidyl esters (GEs) in domestically and commercially prepared food in Singapore and assessed the total dietary exposure for the Singaporean population. Minimal impact on the formation of 3-MCPDEs and GEs was observed from the domestic cooking methods commonly practiced in Singapore such as deep frying and stir frying. The estimated total dietary exposure to 3-MCPDEs for the Singaporean population (aged 15 to 92) was 0.982 µg/kg bw/day for general consumers and 2.212 µg/kg bw/day for high consumers (95th percentile), which accounted for 49.1% and 110.6% of the tolerable dietary intake (TDI) at 2 µg/kg bw/day by the European Food Safety Authority (EFSA). The calculated margins of exposure (MOE) for GEs based on the dietary exposure for general consumers at 0.882 µg/kg bw/day and 2.209 µg/kg bw/day for high consumers were below 10,000, indicating a potential health concern. Our study showed that the occurrence of 3-MCPDEs and GEs varied among vegetable oils, and domestic cooking methods did not significantly impact the levels of 3-MCPDEs and GEs in prepared food. The critical factor influencing the prevalence and occurrence of 3-MCPDEs and GEs was the choice of oil used for cooking, which absorbed into the cooked food. It is essential to encourage the food industry to continue its innovation on mitigation measures to control and reduce 3-MCPDEs and GEs in vegetable oil production. Consumers are advised to make informed choices on food consumption and cooking oil for food preparation to reduce their exposure to 3-MCPDEs and GEs.

Salivary pleomorphic adenoma is histopathologically characterized by its colorful stroma with myxoid, chondroid, and hyaline appearances, due to enhanced biosynthesis of extracellular matrix (ECM) molecules and poor vascularity. Thus, pleomorphic adenoma cells embedded in the stroma typically survive under hypoxic conditions. We determined the expression kinetics of ECM molecules, such as perlecan and fibronectin (FN), under hypoxia in SM-AP1 cells which are duct epithelial differentiated cells, and in SM-AP4 cells, which are myoepithelial differentiated cells, cloned from pleomorphic adenoma of the parotid gland. We investigated hypoxia-inducible factor-1α (HIF-1α)-inducing pathways through a variety of ECM molecules in association with their cellular proliferation and migration. We observed that hypoxic conditions with elevated HIF-1α protein levels induced increased expression of perlecan and FN in SM-AP cells than in controls. Moreover, perlecan and FN knockdown reduced the proliferation of SM-AP1 and SM-AP4 cells under hypoxia. Further, SM-AP1 cell migration was enhanced by both perlecan and FN knockdown, whereas SM-AP4 cell migration was increased by perlecan knockdown and inhibited by fibronectin knockdown. The results indicated that pleomorphic adenoma cells can survive under hypoxic conditions by promoting cell proliferation via enhanced synthesis of ECM molecules. Overall, ECM molecules may be a new anti-tumor target under hypoxic conditions.

This study presents the effects of the microstructure on the corresponding fracture toughness of the Ti–5Al–5Mo–5V–1Cr–1Fe (Ti-55511) alloy by undergoing STA (α/β solution treatment plus aging) and STAA (α/β solution treatment plus first-step aging and second-step aging) processes. The results show that the microstructural evolution of Ti-55511 as well as the corresponding fracture toughness is very sensitive to the heat treatment mechanism. Here, an increase in the solution temperature can reduce the content of αp, while increasing the content of α precipitates during the STA and STAA processes. An increase in the aging temperature coarsens the α precipitate and decreases its content, which deflects the crack and consumes additional energy, thus resulting in an enhanced fracture toughness. Note that the STAA process results in the further formation of α precipitates, which will cause a larger stress concentration that promotes the growth and coalescence of voids; notably, this is inevitably accompanied by a decrease in toughness. In addition, ductile fracture dominated by dimples is mainly experienced in the crack instability and growth stage, and a higher toughness increases the size of the dimples. Overall, this study indicates that the toughness of the alloy can be controlled from 38.2 to 110 MPa⋅m0.5 by multiple heat treatments.

Abstract Background Most of malignant melanomas originate from skin and often metastasize to the lungs, rarely metastasizes to the liver and bone. However, imageology characters of lung metastasis tumor are commonly similar to those of fungal infections. Case presentation A patient was admitted with unhealed plantar puncture wound for 3 years, and cough and expectoration for 2 years. The chest computed tomography (CT) revealed multiple nodules with cavities, and the patient was diagnosed of pulmonary fungal infection in another hospital and received antifungal therapy for more than 8 months, but the clinical symptoms and chest imaging findings continue to progress. After admission, the pathological results of both lung biopsy and biopsy of the plantar wound 3 years ago indicated malignant melanoma. Conclusions The diagnosis of lung lesions cannot rely solely on imaging diagnosis, lung biopsy should be performed if necessary.

Anthocyanins, a categories of metabolites derived from flavonoid biosynthesis pathway, are responsible for the red color of peach. Anthocyanin metabolism is modulated by a complicated regulatory network. In this study, our results demonstrated that PpNAC25 was a component of anthocyanin regulatory network in peach. PpNAC25 showed a closed relationship with the well-known ripening-related gene NOR (no-ripening) and was highly expressed in peach fruit. Overexpression of PpNAC25 in poplar resulted in a redder shoot tips compared with EV control. PpNAC25 overexpression upregulated the anthocyanin biosynthetic and transportation genes in transcript levels and then increased anthocyanin contents. In Y1H and Luc/Ren assay, PpNAC25 bound to the promoter of the anthocyanin-activator PpMYB10.1 and PpMYB10.2 and activated its transcript. Moreover, PpNAC25 formed a heterodimer with PpNAC1, an anthocyanin-regulator. Our researches provide evidence that PpNAC25 may be a positive regulator of anthocyanin biosynthesis in peach fruit.

Computer-generated hologram (CGH) is a diffractive optical element that has been commonly used as wavefront corrector in the interferometer and can be accurately manufactured to meet the requirements of high-precision detection. But the transmitted wavefront error (TWE) of the CGH substrate and the adjustment errors of cylindrical surface are influence factors of high accuracy measurement. In this paper, we propose the method of calibrating the CGH substrate TWE. After calibrating, the substrate TWE is 0.0052λ (RMS). Meanwhile, we obtain the general expression for the misalignment aberrations by describing the relationship between the wavefront of the measured cylindrical mirror and the corresponding adjustment errors. Then the adjustment errors of the test cylindrical surface can be completely separated by subtracting the fitting function from the measurement on the basis of least-square algorithm. After that, the PV value changes from 3.0565λ to 0.9825λ, and the RMS value changes from 0.5278λ to 0.1273λ, the measurement results have been greatly improved.

In this paper, the issue of security sliding mode control (SMC) is addressed for networked nonlinear singular systems with semi-Markov switching parameters via the Takagi-Sugeno (T-S) fuzzy strategy. The goal is to guarantee a good steady performance of dynamical systems under the framework of deception attacks. First, the common sliding surface is proposed to avoid the potential instability caused by repeated jumps of sliding surface. Then, based on the partly unknown transition rate, sufficient conditions are constructed to realize the stochastic admissibility for the corresponding system. Moreover, a fuzzy security SMC law is designed to drive the state trajectory onto the specified sliding region. Finally, a practical example is shown to validate the proposed algorithm.

The present paper examines the temperature sensitivity of tensile twinning in a magnesium single crystal during nanoindentation of the prismatic plane. High temperature indentations from 25 °C to 250 °C were employed on a well polished magnesium single crystal {10-10} plane. For a indentation curve displaying a pop-in, a single twin was seen on the sample surface using Atomic Force Microscopy (AFM) imaging. For indentations that produced no pop-in, no twinning was observed. We thus conclude the pop-in arises from a twinning event in the present case. With increasing temperature, the mean pop-in load (measured from 200 repeat indentations of each testing temperature) drops markedly. This is interpreted by the thermal activation of nucleation of lattice dislocations, which immediately trigger a twinning event. Thermal activation analysis yields activation energies that are consistent with this idea. With increasing temperature the pop-ins became deeper and the twins, after further indentation, showed more growth. It is likely that non-basal slip is activated in the stress concentrations that arise during twinning and the thermal activation of this slip accounts for the observed temperature effects. It is concluded that in interpreting the temperature sensitivity of twinning stresses, any associated lattice dislocation activity must be considered.

The RGB-D-based human action recognition is gaining increasing attention because the different modalities can provide complementary information. However, the recognition performance is still not satisfactory due to the limited ability to learn spatial-temporal feature and insufficient inter-model interaction. In this paper, we propose a novel approach for RGB-D human action recognition by aggregating spatial-temporal information and implementing cross-modality interactive learning. Firstly, a spatial-temporal information aggregation module (STIAM) is proposed to utilizes sample convolutional neural networks (CNNs) to aggregate the spatial-temporal information in entire RGB-D sequence into lightweight representations efficiently. This allows the model to extract richer spatial-temporal features with limited extra memory and computational cost. Secondly, a cross-modality interactive module (CMIM) is proposed to fully fuse the multi-modal complementary information. Moreover, a multi-modal interactive network (MMINet) is constructed for RGB-D-based action recognition by embeding the above two modules into the two-stream CNNs. In order to verify the universality of our approach, two backbones are deployed in the two-stream architecture, successively. Ablation experiments demonstrate that the proposed STIAM can bring significant improvement in recognizing actions. CMIM can further play the advantages of complementary features of multiple modalities. Extensive experiments on NTU RGB+D 60, NTU RGB+D 120 and PKU-MMD datasets proved the effectiveness of the proposed approach. The proposed approach achieves an accuracy of 94.3% and 96.5% for cross-subject and cross-view on NTU RGB+D 60, 91.7% and 92.6% for cross-subject and cross-setup on NTU RGB+D 120, 93.6% and 94.2% for cross-subject and cross-view on PKU-MMD datasets, which are the state-of-the-art performance. Further analysis denotes that our approach has advantages in recognizing subtle actions.

The bottom friction is critical for the dissipation of the global tidal energy. The bottom friction coefficient is traditionally determined using the Manning’s n formulation in tidal models. The Manning’s n coefficient in the Manning’s n formulation is vital for the accurate simulation and prediction of the tide in coastal shallow waters, but it cannot be directly measured and contains large amounts of uncertainties. Based on a two-dimensional multi-constituent tidal model with the adjoint data assimilation, the estimation of the Manning’s n coefficient is investigated by assimilating satellite observations in the Bohai, Yellow and East China Seas with the simulation of four principal tidal constituents M2, S2, K1 and O1. In the twin experiments, the Manning’s n coefficient is assumed to be constant, and it is estimated by assimilating the synthetic observations at the spatial locations of the satellite tracks. Regardless the inclusion of artificial random observational errors associated with synthetic observations, the model performance is improved as evaluated by the independent synthetic observations. The prescribed ‘real’ Manning’s n coefficient is reasonably estimated, indicating that the adjoint data assimilation is an effective method to estimate the Manning’s n coefficient in multi-constituent tidal models. In the practical experiments, the errors between the independent observations at the tidal gauge stations and the corresponding simulated results of the four principal tidal constituents are substantially decreased under both scenarios of the constant and spatially-temporally varying Manning’s n coefficient estimated by assimilating the satellite observations with the adjoint data assimilation. In addition, the estimated spatial and temporal variation trend is robust and not affected by the model settings. The spatially-temporally varying Manning’s n coefficient is negatively correlated with the current speed and shows significant spatial variation in the shallow water areas. This study demonstrates that the Manning’s n coefficient can be reasonably estimated by the adjoint data assimilation, which allows significant improvement in accurate simulation of the ocean tide.

We studied the work function of cesium deposition and co-deposition with the electronegative element on the plasma grid (PG) using the first-principles calculations. The impurity particles may exist in the background plasma and vacuum chamber wall, and the work function of the PG will be affected. The results indicate that the minimum work functions of pure cesium deposition on Mo (110), W (110), and Mo (112) are reached at a partial monolayer. They are 1.66 eV (σ = 0.56 θ), 1.69 eV (σ = 0.75 θ), and 1.75 eV (σ = 0.88 θ), respectively. An appropriate co-deposition model consisting of cesium with electronegative elements can further decrease the work function. The coverage of cesium and electronegative elements are both 0.34 θ in all the co-deposition models. The F-Cs co-deposition model where the Cs atom and F atom are aligned along the surface normal obtains the lowest work function. They are 1.31 eV for F-Cs on Mo (110), and 1.23 eV for F-Cs on W (110), respectively. The change in work function is linearly related to the change in dipole moment density with a slope of −167.03 VÅ. For pure cesium deposition, two factors control the change in dipole-moment density, one is the electron transfer between adsorbates and the substrate, and another one is the restructuring of surface atoms. There are two additional factors for the co-deposition model. One is the intrinsic dipole moment of the double layer, the other is the angle between the intrinsic dipole moment and the surface. The latter two factors play important roles in increasing the total dipole moment.