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507 results on '"Jin, Denan"'

82. Hypermetabolism of glutathione, glutamate and ornithine via redox imbalance in methylglyoxal-induced peritoneal injury rats.

Author

Hirahara, Ichiro, Kusano, Eiji, Jin, Denan, and Takai, Shinji

Subjects
METHIONINE, ORNITHINE, ADVANCED glycation end-products, GLUTAMIC acid, RATS, GLUTATHIONE peroxidase
Abstract

Peritoneal dialysis (PD) is a blood purification treatment for patients with reduced renal function. However, the peritoneum is exposed to oxidative stress during PD and long-term PD results in peritoneal damage, leading to the termination of PD. Methylglyoxal (MGO) contained in commercial PD fluids is a source of strong oxidative stress. The aim of this study was to clarify the mechanism of MGO-induced peritoneal injury using metabolome analysis in rats. We prepared peritoneal fibrosis rats by intraperitoneal administration of PD fluids containing MGO for 21 days. As a result, MGO-induced excessive proliferation of mesenchymal cells with an accumulation of advanced glycation end-products (AGEs) at the surface of the thickened peritoneum in rats. The effluent levels of methionine sulfoxide, an oxidative stress marker and glutathione peroxidase activity were increased in the MGO-treated rats. The levels of glutathione, glutamate, aspartate, ornithine and AGEs were also increased in these rats. MGO upregulated the gene expression of transporters and enzymes related to the metabolism of glutathione, glutamate and ornithine in the peritoneum. These results suggest that MGO may induce peritoneal injury with mesenchymal cell proliferation via increased redox metabolism, directly or through the formation of AGEs during PD. [ABSTRACT FROM AUTHOR]

Published
2020
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89. Effects of mitomycin C on the expression of chymase and mast cells in the conjunctival scar of a monkey trabeculectomy model

Author

Okada, Kouhei, Sugiyama, Tetsuya, Takai, Shinji, Jin, Denan, Ishida, Osamu, Fukmoto, Masanori, Oku, Hidehiro, Miyazaki, Mizuo, and Ikeda, Tsunehiko

Subjects
genetic structures, Fibrillar Collagens, Mitomycin, digestive, oral, and skin physiology, Trabeculectomy, Haplorhini, Immunohistochemistry, eye diseases, Gene Expression Regulation, Enzymologic, Placebos, Cicatrix, Chymases, Proliferating Cell Nuclear Antigen, Models, Animal, Cell Adhesion, Animals, sense organs, Mast Cells, Conjunctiva, Intraocular Pressure, Research Article
Abstract

Purpose To determine the effects of mitomycin C (MMC) on the expression of chymase and mast cells in the conjunctival scar after trabeculectomy. Methods Ten eyes of five monkeys were used. Three eyes underwent trabeculectomy with MMC (MMC-treated), four eyes had trabeculectomy without MMC (placebo-treated), and three eyes served as control eyes. Intraocular pressure was measured before and three weeks after surgery. The scores of the degree of conjunctival adhesion were evaluated. Immunohistochemistry was used to analyze the densities of proliferative cell nuclear antigen-positive cells, chymase-positive cells, and mast cells. The ratio of collagen fiber areas to conjunctival and scleral lesions was analyzed by Mallory-Azan staining. Results After trabeculectomy, the intraocular pressure reduction of MMC-treated eyes was significantly different from placebo-treated and control eyes (p=0.032, 0.035). The adhesion score of MMC-treated eyes was also significantly lower than that of placebo-treated eyes (p=0.034). Densities of proliferative cell nuclear antigen-positive cells, chymase-positive cells, and areas of collagen fiber in conjunctival and scleral lesions were significantly decreased in MMC-treated eyes, compared with placebo-treated eyes (p=0.034, 0.034, 0.049, respectively). There was a tendency for the density of mast cells to be suppressed in MMC-treated eyes (p=0.157). Conclusions Chymase might be involved in one of the mechanisms by which MMC suppresses scar formation after trabeculectomy.

Published
2009

93. Mo1462 Effect of Recombinant Human Soluble Thrombomodulin in Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Failure in Mice

Author

Osumi, Wataru, primary, JIn, Denan, additional, Imai, Yoshiro, additional, Miyaoka, Yuta, additional, Tashiro, Keitarou, additional, Komeda, Koji, additional, Hirokawa, Fumitoshi, additional, Hayashii, Michihiro, additional, Takai, Shinji, additional, Nishiguchi, Kanji, additional, and Uchiyama, Kazuhisa, additional

Published
2016
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