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Background: The value of rapid reviews in informing health care decisions is more evident since the onset of the coronavirus disease 2019 (COVID-19) pandemic. While systematic reviews can be completed rapidly, rapid reviews are usually a type of evidence synthesis in which components of the systematic review process may be simplified or omitted to produce information more efficiently within constraints of time, expertise, funding or any combination thereof. There is an absence of high-quality evidence underpinning some decisions about how we plan, do and share rapid reviews. We will conduct a modified James Lind Alliance Priority Setting Partnership to determine the top 10 unanswered research questions about how we plan, do and share rapid reviews in collaboration with patients, public, reviewers, researchers, clinicians, policymakers and funders. Methods: An international steering group consisting of key stakeholder perspectives (patients, the public, reviewers, researchers, clinicians, policymakers and funders) will facilitate broad reach, recruitment and participation across stakeholder groups. An initial online survey will identify stakeholders’ perceptions of research uncertainties about how we plan, do and share rapid reviews. Responses will be categorised to generate a long list of questions. The list will be checked against systematic reviews published within the past three years to identify if the question is unanswered. A second online stakeholder survey will rank the long list in order of priority. Finally, a virtual consensus workshop of key stakeholders will agree on the top 10 unanswered questions. Discussion: Research prioritisation is an important means for minimising research waste and ensuring that research resources are targeted towards answering the most important questions. Identifying the top 10 rapid review methodology research priorities will help target research to improve how we plan, do and share rapid reviews and ultimately enhance the use of high-quality synthesised evidence to inform health care policy and practice.

This work introduces a novel sieving gel for DNA electrophoresis using a classical click chemistry reaction, the copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC), to cross-link functional polymer chains. The efficiency of this reaction provides, under mild conditions, hydrogels with near-ideal network connectivity and improved physical properties. Hydrogel formation via click chemistry condensation of functional polymers does not involve the use of toxic monomers and UV initiation. The performance of the new hydrogel in the separation of double stranded DNA fragments was evaluated in the 2200 TapeStation system, an analytical platform, recently introduced by Agilent that combines the advantages of CE in terms of miniaturization and automation with the simplicity of use of slab gel electrophoresis. The click gel enables addition of florescent dyes prior to electrophoresis with considerable improvement of resolution and separation efficiency over conventional cross-linked polyacrylamide gels.

On September 2, 2015, two towing vessels collided on the Lower Mississippi River at Mile 937, near Columbus, Kentucky, resulting in the complete breach of the #3 starboard cargo tank on the T/B APEX 3508 and the release of 120,588 gallons of clarified slurry oil (CSO; Group V oil; Specific Gravity: 1.14) into the navigable waterway. The incident was classified as a Major Inland Spill in accordance with the National Oil and Hazardous Substance Contingency Plan and a Major Marine Casualty that was jointly investigated by the United States Coast Guard and the National Transportation Safety Board. Over flights conducted as far as 20 miles downriver indicated only light, sporadic sheening for 1–2 days. On-water and shoreline assessments conducted up to six miles downriver revealed no visible signs of surface oiling. Based on its properties, the vast majority of the CSO was presumed to have sunk, but its precise disposition and location was not confirmed. Using side scan sonar (SSS) technology, two distinct subsurface anomalies with an approximate combined area of 9,200 m2 were identified on the river bed in the vicinity of the incident. The anomalies were confirmed as oil by divers and direct sampling, and were then divided into 25 m grids for identification and tracking. The Unified Command evaluated best available technologies and determined that GPS guided environmental dredging would be the safest, most effective and efficient of the recovery options. The established cleanup endpoint was a maximum of 10% sporadic oil distribution in each grid. Two endangered mussel species were identified as potentially inhabiting the affected area. A diver survey was conducted in the area and concluded that bottom habitat was not likely to support the listed species. Further consultations with the resource manager indicated that proposed recovery operations posed low risk to the species. Recovery operations commenced on September 15, 2015 and concluded on September 25, 2015. Endpoint verification was conducted via SSS. In total, response operations lasted 23 days (eight operational periods), involved over 120 responders and 75 specialized response assets, and cost approximately $5 million. Approximately 2,524 m3 of dredged material (liquid and solids) were removed. After decanting, approximately 1,730 m3of oiled solids representing approximately 50 to 75% of the spilled product was recovered. This case serves as a benchmark for sunken oil detection and recovery operations, and identified many best practices that should be considered on future cases with similar spill conditions.

In December 2015, operations were successfully completed in the recovery of a highly toxic cargo from the sunken tank barge ARGO in Lake Erie. The ARGO, constructed in 1911, sank in 1937 with a cargo of benzol that contained a high percentage of the carcinogen benzene. The ARGO was previously listed as the highest environmental risk in the Great Lakes by the National Oceanic and Atmospheric Administration’s Remediation of Underwater Legacy Environmental Threats (RULET) study. To recover the cargo, salvors designed a diver directed hot-tapping and pumping system to pump the remaining high benzene cargo from the sunken barge at a depth of approximately 50-feet below the lake’s surface. The cargo off-load system included pumping the cargo to a series of storage tanks onboard a barge equipped with designed-for-purpose inert gas and vapor recovery systems to ensure the safety of the public and responders. Working in a Unified Command that included the Coast Guard, U.S. and Ohio Environmental Protection Agencies and National Oceanic Atmospheric Administration, the salvage crew achieved all operational objectives – from safely conducting around-the-clock cold-water contaminated water diving operations to collecting environmental and barge hull samples for further analysis. The ARGO case study will provide lessons learned to assist future responders in safely performing subsea oil removal operations. Additionally, the case study will frame the discussion of current submerged oil recovery regulations and guidance, including the 2016 American Petroleum Institute (API) sunken oil detection and recovery guidance and the U.S. Coast Guard’s guidance on the classification of Oil Spill Removal Organizations (OSRO) that perform non-floating oil detection and recovery operations.

Plain English summary Researchers who conduct studies in health and social care are encouraged to involve the public as early as possible in the process of designing their studies. Before their studies are allowed to start researchers must seek approval from a Research Ethics Committee, which will assess whether the study is going to be safe and ethical for patients or healthy volunteers to take part in. The process of ethical review does not consider how researchers work with patients and the public early on to design their studies. Furthermore, there is no requirement for researchers to seek ethical approval for public involvement. However, in our work advising researchers about public involvement we have found that the ways in which researchers involve the public in the design of their studies are sometimes unintentionally unethical, and this is the focus of our paper. We have observed ten areas where ethical issues may arise because of the actions researchers may or may not take and which might consequently have a negative impact. Therefore, we have used these observations to develop a “framework” to help researchers and the public work together at the early design stage in ways that are ethical. Our intention for the framework is to help researchers be mindful of these ten areas and how easily ethical issues can arise. The framework suggests some ways to overcome the potential issues in each of the ten areas. The ten areas are: 1) Allocating sufficient time for public involvement; 2) Avoiding tokenism; 3) Registering research design stage public involvement work with NHS Research & Development Trust Office at earliest opportunity; 4) Communicating clearly from the outset; 5) Entitling public contributors to stop their involvement for any unstated reasons; 6) Operating fairness of opportunity; 7) Differentiating qualitative research methods and public involvement activities; 8) Working sensitively; 9) Being conscious of confidentiality and 10) Valuing, acknowledging and rewarding public involvement. We looked to see whether any other similar approaches to helping researchers address potential ethical issues when working with the public on designing studies have been published and to our knowledge none exist. Our framework is presented as a draft and believe that it would now benefit from input from researchers and the public to gauge how useful it is and whether there are any other possible situations that it might need to cover. Abstract The current paper highlights real life examples of how ethical issues can arise during public involvement activities at the research design stage. We refer to “the research design stage” as the time between the generation of the research ideas and when formal permissions to start the work including ethical approval are granted. We argue that although most researchers work ethically at this early stage, some may still benefit from being informed about ethically conscious approaches to involving the public. The paper highlights 10 ethical issues that we have observed with involving the public at the research design stage. We provide examples of these observed scenarios to illustrate the issues and make suggestions for how they can be avoided to help researchers become more ethically conscious when involving the public at the research design stage. Currently the draft framework comprises: 1) Allocating sufficient time for public involvement; 2) Avoiding tokenism; 3) Registering research design stage public involvement work with NHS Research & Development Trust Office at earliest opportunity; 4) Communicating clearly from the outset; 5) Entitling public contributors to stop their involvement for any unstated reasons; 6) Operating fairness of opportunity; 7) Differentiating qualitative research methods and public involvement activities; 8) Working sensitively; 9) Being conscious of confidentiality and 10) Valuing, acknowledging and rewarding public involvement. The draft framework will help researchers to recognise the ethical issues when involving the public and is intended to be used voluntarily in a self-regulatory way. We believe that the draft framework requires further consultation and input from the wider research community and the public before endorsement by national UK bodies such as INVOLVE and the Health Research Authority (HRA).

The marine salvage industry plays a vital role in protecting the marine environment. Governments, industry and the public, worldwide, now place environmental protection as the driving objective, second only to the safety of life, during a marine casualty response operation. Recognizing over 20 years after the passage of the Oil Pollution Act of 1990 that the effectiveness of mechanical on-water oil recovery remains at only about 10 to 25 percent while the international salvage industry annually prevents over a million tons of pollutants from reaching the world's oceans, ten years ago the United States began implementing a series of comprehensive salvage and marine firefighting regulations in an effort to improve the nation's environmental protection regime. These regulations specify desired response timeframes for emergency salvage services, contractual requirements, and criteria for evaluating the adequacy of a salvage and marine firefighting service provider. In addition to this effort to prevent surface oil spills, in 2016, the U.S. Coast Guard also recognized the salvage industries advancements in removing oil from sunken ships and recovering submerged pollutants, issuing Oil Spill Removal Organization (OSRO) classification standards for companies that have the capabilities to effectively respond to non-floating oils. Ten years after the implementation of the U.S. salvage and marine firefighting regulatory framework, this paper will review the implementation of the U.S. salvage and marine firefighting regulations and non-floating oil detection and recovery requirements; analyze the impacts and effectiveness of these new policies; and present several case studies and recommendations to further enhance salvage and oil spill response effectiveness.

Objectives To identify which information items potential participants and research nurses rank as the most important, and the reasons for this, when considering participation in a randomised controlled trial. Design Q-methodology approach alongside a think-aloud process. Using a vignette outlining a hypothetical trial, participants were asked to rank statements about informational items usually included in a participant information leaflet (PIL) on a Q-grid, while undertaking a real-time think-aloud process to elicit the underpinning decision processes. Analysis of quantitative data was conducted using descriptive statistics and qualitative data was coded using content analysis. Participants 20 participants (10 potential trial participants and 10 research nurses). Setting UK-based participants. Results Ten research nurses and 10 potential trial participants provided data for the study. Both stakeholder groups ranked similar statements in their top three most important statements, with ‘What are the possible disadvantages and risks of taking part?’ featuring in both. However, considerable variability existed between the groups with regard to their ranking of statements of least importance. Participants identified that sufficient information to make a decision was secured using around 14 items. Participants also identified other items of importance not routinely included in PILs. Conclusions This study has provided a unique insight into how and why different trial stakeholder groups rank informational items currently contained within PILs. These results have implications for those developing future PILs and those who develop guidance on their content; PILs should focus most on the information items that potential trial participants want and need to make an informed choice about trial participation.

Researchers carrying out research in the NHS in England have to obtain approval for their study from an NHS Research Ethics Committee (REC). Involving the public in research helps to ensure studies are ethically acceptable to the people taking part, and therefore supports the REC review. The form used by RECs asks researchers to describe any involvement that has taken place before the review or any planned for the future. We analysed researchers’ reports of involvement in 2748 applications to RECs in 2014, to assess how well their approaches to involvement are informing the review process. We found that researchers rarely describe involvement in enough detail to help REC members. It is difficult to judge whether previous involvement has shaped the research design in any way, and whether plans for future involvement are meaningful. It also seems that some researchers remain unclear about involvement and its purpose at different stages. This may be severely limiting its impact. So that public involvement can usefully inform REC reviews in future, the Health Research Authority, which oversees RECs, will carry out further work to find out what information RECS need about involvement. This information will be used to change the application form and to develop guidance and training for REC members and the wider research community. Researchers may also benefit from clearer guidance on the value and purpose of involvement at key research stages: early design, data collection and the dissemination of results. Background Researchers conducting research in the NHS in England are required to submit their study for approval by an NHS Research Ethics Committee (REC). Public involvement in research prior to REC review helps to ensure studies are ethically acceptable to participants, thus informing the review process. The Integrated Research Application System (IRAS) used by RECs, asks researchers to describe any involvement in the development of their project, and in its delivery and dissemination. We analysed researchers’ reports of involvement to assess how well current approaches to involvement are supporting REC review. Methods We used a mixed methods approach. The anonymised free-text data from all 2748 non-educational applications submitted to RECs in 2014 were analysed using NVivo. Themes were developed from the data and used to summarise and categorise the different types of reports of involvement. The frequency of common types of report was analysed using simple statistics. Results In general, researchers rarely describe any prior involvement in sufficient detail to know what was done and what difference this made. This makes it difficult to judge whether the involvement shaped the research design in any way to make it more ethically acceptable. Similarly, researchers’ plans for future involvement are not clear enough to enable RECs to make a proper assessment of whether this involvement will be meaningful, or whether potential ethical concerns raised by involvement have been addressed. This analysis also shows there is still considerable misunderstanding amongst researchers around what involvement means, and its purpose at different stages of a project. This may be severely limiting the potential for impact. Conclusions So that public involvement can usefully inform REC review in future, the HRA is undertaking a collaborative exercise to understand what information RECS need about involvement, and what changes need to be made to the IRAS form. At the same time it will develop guidance and training for REC members and the wider research community about how public involvement can support ethical review. Researchers may also benefit from guidance on the value and purpose of involvement at the research stages: design, data collection and dissemination of results.

The marine salvage and commercial diving industries have increasingly been sought out to prevent oil spills from submerged shipwrecks, and to detect and recover spilled oil below the surface once a subsea spill occurs. In recent years, underwater oil recovery techniques have advanced from predominantly surface-supplied diver installed vacuum or pumping systems in relatively shallow waters to the use of saturation diving systems and remotely operated vehicles at greater depths. Underwater oil detection technologies have advanced permitting the detection of spilled oil in the water column, on the bottom and in the subsurface. For oil trapped within a sunken shipwreck, neutron backscatter technology has been successfully applied to locate oil inside the ship. Additionally, the International Maritime Organization, U.S. Coast Guard and National Oceanic and Atmospheric Administration have published regulations, guidance and studies in the past five years in an effort to improve submerged oil detection and recovery operations. This technical paper will provide an overview of the regulatory framework, basics of underwater oil spill response operations and an analysis of recent technological advances available to detect and recover oil at depth. Multi-beam sonar, real-time mass spectrometry, saturation diving systems, diver-operated recovery systems, and remotely operated vehicle systems will be discussed. Recent case studies will frame the presentation of advances in subsea oil detection and recovery equipment. Finally, conclusions and recommendations will be presented to further advance submerged oil detection and recovery efficiency and effectiveness.

Moored passive acoustic detectors (e.g. C-PODs) are widely used to study harbour porpoise Phocoena phocoena distribution and relative abundance, but their use in tidal-stream habitats is complicated by the need for retrievable flow-resistant seabed fixings and the occur- rence of flow-induced noise in the resultant data. In this study, we explored the use of a new method aimed at tidal-stream habitats, which are of increasing interest for marine renewable energy generation. Porpoise detectors (C-PODs) were attached to multiple drifters and repeatedly set adrift at a tidal-stream site in western Scotland during May 2010 and August 2011. Porpoise vocalisations were successfully detected under varying tidal conditions during approximately 63 h of drifting. Harbour porpoise distribution, as determined by the drifting detectors, was similar to that found using the traditional, yet more logistically intensive, visual and acoustic boat-based sur- veys and to an extent that found by moored C-PODs. Drifting detectors also mapped tidally driven spatiotemporal variability in ambient noise levels which could influence porpoise detection. In summary, drifters equipped with passive acoustic detectors offer a new, rapid and inexpensive tool for investigating porpoise occurrence and behaviour in tidal-stream habitats, and should be considered as part of a comprehensive marine mammal monitoring approach of these energetic environments in the context of marine renewable energy development and other industries.

The authors acknowledge support received from the Scottish Funding Council, Highlands & Islands Enterprise, Scottish Power Renewables and E.ON under the Hebridean Marine Energy Futures (HMEF) programme (Grant reference number 1R042 (HFU) SPIRIT , Project Ref. HR10012), a research initiative led by the University of the Highlands and Islands (UHI) and Aquamarine Power. Scarba C-POD data were collected under the UK Natural Environment Research Council (DEFRA/NERC) grant NE/J004367/1 (RESPONSE). Marine habitat heterogeneity underpins species distribution and can be generated through interactions between physical and biological drivers at multiple spatiotemporal scales. Passive acoustic monitoring (PAM) is used worldwide to study potential impacts of marine industrial activities on cetaceans, but understanding of animals’ site use at small spatiotemporal scales (

While patient and public involvement (PPI) in health and social care research has progressed successfully in the last decade, a range of difficulties with the evidence base exist, including poor understanding of the concept of impact, limited theorization and an absence of quantitative impact measurement. In this paper, we argue that a paradigm change towards robust measurement of the impact of involvement in research is needed to complement qualitative explorations. We argue that service users should be collaboratively involved in the conceptualization, theorization and development of instruments to measure PPI impact. We consider the key advantages measurement would bring in strengthening the PPI evidence base through a greater understanding of what works, for whom, in what circumstances and why.

Background:? The involvement of service users in the design and conduct of health research has developed significantly in the UK in recent years. Involving service users is now seen as a core component of good research practice for all forms of health research. Given the important role that users have in health research, it is necessary to develop guidelines for their effective involvement. Whilst guidelines are currently being formulated, there remain no criteria with which to assess user involvement in published studies and funding applications. Objective:? This study offers guidelines for appraising the quality and impact of user involvement in published papers and grant applications. Methods:? Appraisal guidelines for user involvement have been developed on the basis of available literature and experiences from studies involving cancer patients and carers in the design and conduct of research. Findings:? Nine appraisal criteria have been developed. Criteria include issues such as ‘Is the rationale for involving users clearly demonstrated?’, ‘Is the level of user involvement appropriate?’, ‘Is the recruitment strategy appropriate?’, and ‘Is the nature of training appropriate?’ Conclusion:? Generating and applying guidelines is vital if the impact of user involvement agenda in health research is to be understood.

Historically, once a ship sank below the surface, the oil that remained onboard became inaccessible to responders. In the last few years, however, there has been a significant push by both regulators and salvors to go deeper below the surface to recover oil trapped within a shipwreck. Recent examples in the United States include the recovery of oil from the SS JACOB LUCKENBACH in a water depth greater than 175-feet seawater (fsw) and the attempt to recover oil from the T/V BOW MARINER at a depth greater than 265 fsw. This technical paper provides an analysis of recent technological advances available to recover oil at depth, from using saturation diving systems and manually connected viscous oil pumping systems to remotely operated vehicles and associated offloading systems. Recent case studies frame the presentation of advances in sub-sea oil recovery equipment and associated safety, logistical and financial considerations.

Various initiatives have been implemented in recent years to support primary care practitioners new to research. We describe the evaluation of one particular scheme, the NHS Eastern Region Primary Care RD Enterprise Award Scheme, and discuss the implications of the results for future policy.Representatives from both the funders (NHS RD) and the award holders were involved in the design of an evaluation questionnaire and in the interpretation of the results.and Discussion. The evaluation demonstrated value in relation to traditional research outcomes and also, notably, in relation to professional contribution. Future policies may need to address: the indicators used in measuring the success of such schemes; the relationship between what individuals choose to do and its context within national policy on research and development; and the sustainability of involvement in research.

1. Sodium fluoride causes apoptosis of pancreatic beta-cells and this response is enhanced by pre-treatment with pertussis toxin. In the present study, tyrosine kinase inhibitors were used to investigate the mechanisms of action of NaF and pertussis toxin in the beta-cell line, RINm5F. 2. Exposure of RINm5F cells to low concentrations of genistein or tyrphostin A25 resulted in significant inhibition of cell death induced by 5 mM NaF. Higher concentrations (>25 microM) were cytotoxic in the absence of NaF but, paradoxically, the combination of genistein and NaF induced less cell death than when each agent was used alone. 3. The increase in cell death induced by 100 microM genistein was markedly inhibited by ciprofloxacin, a drug which binds to topoisomerase II. Etoposide (which inhibits topoisomerase II but has no effect on tyrosine kinase activity) also caused an increase in RINm5F cell death. Neither etoposide nor ciprofloxacin altered the response to 5 mM NaF. 4. Pertussis toxin markedly enhanced the extent of RINm5F cell death induced by NaF and this effect was completely prevented by 25 microM genistein. The inhibition caused by genistein was not affected by ciprofloxacin but was reproduced by a structurally dissimilar tyrosine kinase inhibitor, herbimycin A. 5. The results demonstrate that RINm5F beta-cells express a pertussis toxin sensitive pathway that is anti-apoptotic. The activity of this pathway is most evident in cells exposed to pro-apoptotic stimuli where the effects of pertussis toxin can be blocked by inhibitors of tyrosine kinase enzymes. A genistein-sensitive tyrosine kinase does not appear to be involved in RINm5F cell survival under basal conditions.

Guidelines for achieving change in the performance of a task are of little value unless they are actually adopted and used by both planning and operational staff. This suggests that success—or rather lasting improvement—is dependent on appropriate processes for guideline development. The processes need to build broad-based support for lasting cultural or institutional change. The authors were involved in a recent attempt to change the approach to flood warnings in Australia by drawing up a set of `best practice' guidelines. This paper explores the background to change, the institutional context, the process of achieving agreement on, and building commitment for, the details of the changes, and the need for further continuous effort to ensure implementation of the changes on the ground.

Each year, a large number of children change residence and school. This paper examines the social experience of being a newcomer at secondary school, with special reference to the process of adjustment. It is based on a detailed longitudinal case study of 45 newcomers. Each was followed for 12 months, with the main source of data being seven in-depth interviews with each student, backed up by unscheduled interviews, school reports, teacher and peer-group comments and observations. Because the data show extensive similarity in newcomers' experiences–all must deal with a different curriculum, new teachers and the social world of other students–it is possible to construct a general three-stage map of the process of adjustment. This map has important implications for the guidance and counselling of such students.

Noise in hospitals frequently exceeds recommended levels and has detrimental psychological and physiological effects on patients and staff. The aim of this study was to record the noise levels within the cardiac surgical intensive care unit (CSICU) environment in secret. The device used was a CEL Instruments environmental noise meter concealed in a dummy box featuring temperature and humidity digital displays. It allowed greater than 16 hours recording time at a 1-minute resolution. The 24-hour period was covered by overlapping recordings. The data collected were downloaded onto a personal computer for analysis. The maximum sound level recorded was 100.9 decibel level (dBA), 1 min Lmax (the maximum sound level occurring in a 1-min period). The minimum sound level was 61.3 dBA 1 min Lmax. The continuous background noise was at its lowest at 57.5 dBA 1 min equivalent continuous sound pressure levels (Leq) and at its peak 77.3 dBA 1 min Leq. Noise in the CSICU was above the recommended levels for patients and staff well-being. Future studies will be designed to establish a correlation between sound levels and events.

This paper presents a novel approach to the construction of an adaptive filter making use of the so-called "distributed arithmetic" filter architecture originally suggested by Peled and Liu [7] for the realization of fixed response digital frequency filters. The technique uses only the operations of memory access, addition, and scaling, without the need for digital multiplication. Since multiplication is often quoted as the major bottleneck in digital signal processing structures, the system derives considerable advantage by the exclusion of this operation. The paper presents the derivation of a new adaptive algorithm based on this particular hardware structure, although no rigorous theoretical proof of the algorithm convergence properties is given. Computer simulations are included to demonstrate some of the basic operational characteristics of the structure. Finally, results from a hardware prototype, constructed using standard TTL integrated circuits, are presented. This approach differs from contemporary ideas which depend on the use of digital multipliers in either custom VLSI designs or using standard signal processing chips. It offers high-bandwidth operation at low cost using devices which are already in great demand by the computer market. Alternatively, the algorithm is ideal for implementation as a microprocessor-based system which could operate on real-time voice-bandwidth signals with a minimum of peripheral interface circuitry.