51. Antibiotic resistance genes (ARGs) in duck and fish production ponds with integrated or non-integrated mode
- Author
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Jinliang Chen, Jia-Xin Xu, Qingping Du, Jia-Yin Ling, Yanbin Xu, Jia-Le Zhou, Li Zheng, and Lu Huang
- Subjects
0301 basic medicine ,China ,Geologic Sediments ,Veterinary medicine ,Environmental Engineering ,Tetracycline ,Health, Toxicology and Mutagenesis ,Fish farming ,Aquaculture ,010501 environmental sciences ,Integron ,01 natural sciences ,03 medical and health sciences ,Bacterial Proteins ,stomatognathic system ,Abundance (ecology) ,parasitic diseases ,polycyclic compounds ,medicine ,Animals ,Environmental Chemistry ,Water Pollutants ,Ponds ,Relative species abundance ,0105 earth and related environmental sciences ,biology ,business.industry ,Ecology ,fungi ,Fishes ,Public Health, Environmental and Occupational Health ,Drug Resistance, Microbial ,General Medicine ,General Chemistry ,biochemical phenomena, metabolism, and nutrition ,Pollution ,Ducks ,030104 developmental biology ,Genes, Bacterial ,biology.protein ,Monoculture ,business ,Environmental Monitoring ,medicine.drug ,Antibiotic resistance genes - Abstract
Antibiotic resistance genes (ARGs) are emerging micropollutants with environmental persistence. Aquaculture environments are considered as potential reservoirs for ARGs pollution and horizontal gene transfer (HGT). This study analyzed water and sediment from eight culture ponds (integrated culture: duck-fish pond; monoculture: duck pond and fish pond) and a control pond (without any aquaculture activity) in Zhongshan, South China. Seventeen types of ARGs were detected in all ponds, which conferring resistance to four classes of antibiotics including tetracycline ( tet A, tet B, tet C, tet E, tet G, tet L, tet A-P, tet M, tet O, tet S, tet W and tet X), AmpC beta-lactamase products ( EBC and FOX ), sulfonamide ( sul 1 and sul 2) and erythromycin ( erm A), with class 1 integron ( intI 1) as motility gene. The total concentrations of detected ARGs in culture pond water were much higher than control (about 1.6–4.0 times). Integrated culture showed lowest absolute abundance of ∑ARGs in water (3.686 × 10 7 copies mL −1 ) and the highest in sediment (4.574 × 10 8 copies g −1 ). Monoculture ponds showed higher relative abundance of ∑ARGs both in water (fish pond: 0.5149) and sediment (duck pond: 0.4919). As the main contributor to the ARGs abundance and significant correlations with ∑ tet , ∑ARGs and intI 1 ( P tet A was suggested to be a potential indicator for the abundance of tetracycline resistance genes in these classes of aquaculture modes in the Pearl River Delta. This study provides a case for the ARGs abundance in aquaculture and as a reference for the upcoming health risk assessment in aquatic environment.
- Published
- 2017