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We present a genome assembly from an individual Aricia artaxerxes (the northern brown argus; Arthropoda; Insecta; Lepidoptera; Lycaenidae). The genome sequence is 458 megabases in span. Most of the assembly (99.99%) is scaffolded into 23 chromosomal pseudomolecules, including the assembled Z sex chromosome. The mitochondrial genome has also been assembled and is 15.8 kilobases in length. Gene annotation of this assembly on Ensembl has identified 12,688 protein coding genes.

The principal goals of the study are to implement an adjusted.ersion of the well known Bogardus scale of social distance and also to orientate senior undergraduates regarding research techniques. The scope of the project is limited and stress is laid on the qualitative aspects of the sample. In discussing the results certain comparisons are made with the work of Lever (1968; 1979) and the most important variable correlated with interethnic attitude is the respondent's school. The results of the study indicate that Afrikaans respondents are the most exclusive group, whilst Coloured respondents are the most inclusive in attitude. In comparing the material with that of Lever, a tendency to narrow their distance regarding other population groups is indicated for the former group Differences between the attitudes of English speaking and Afrikaans speaking respondents indicate the exclusion of a simple relationship between inter-ethnic communication on the one hand and pronounced attitudes on the other hand

Background: The sustainable development goal (SDG) 4.2 calls to ‘ensure that all girls and boys have access to quality early childhood development, care and preprimary education so that they are ready for primary education’. It is then important to identify early childhood literacy drivers for better decision-making in education. Objectives: This research investigates the impact of pre-schooling and home environment effect on children’s literacy in sub-Saharan Africa francophone countries. Method: A total of 21 933 Grade 2 children participated in the regional assessment of literacy in 14 countries. Items on cognitive skills were used to assess children’s literacy skill. A contextual questionnaire was used to collect data on the home environment and children’s characteristics. Descriptive statistics, logistic regression and inverse-probability-weighted regression adjustment were used to estimate the impact of pre-schooling and home environment on children’s literacy skill. Results: At least 57% of children in Grade 2 did not have the literacy skill required to pursue their learning without difficulty. Only 28% of children attended preschool. Home-language and reading at home have the highest effect size on children’s literacy skill. Attending preschool improves the percentage of children capable to pursue their learning without difficulty by 10%. Furthermore, that improvement is 15.7% for children who attended preschool. Impact of pre-schooling varies between countries and slightly across gender. Conclusion: Findings of this research call for better access to pre-schooling and better home environment to improve children’s literacy skill. The research will contribute to efforts of the sub-Saharan Africa francophone countries to achieve the SDG 4.2. Contribution: This research contributed to fill the knowledge gaps on Early Childhood literacy in the Global South. It highlighted home environment drivers and the impact of preschooling on children’s literacy skills in the Global South.

The measurement of a stocks return over a time period is analysed for several reasons. The most obvious and most important one is to inform investors’ expectations regarding future earnings potential. Therefore, the study made a comparison using the financial performance of single-listed shares versus dual-listed shares that trade primarily on the South African stock market (JSE). The time-period for the comparison of financial performance of single- and dual-listed shares was from 2005 to 2020 to confirm or refute the general perception surrounding superior returns of dual-listed companies as opposed to single-listed companies. Utilising financial ratios can be imperative when making informed judgments about investment portfolios. Seven of the most important financial ratios were used to measure the performance of company shares within nine specified industry sectors in South Africa. These included the earnings per share ratio (EPS), price-earnings ratio (P/E), market to book value ratio (M/B), current ratio (CR), debt to equity ratio (DER), and the return on equity ratio (ROE). The nine identified industries included the transport, consumer staples, printing, pharmaceutical, mining and manufacturing, technology, luxury goods and services, financial services, and real estate industries. The results indicate that the dual-listed companies do indeed outperform single-listed companies on the JSE for the majority of the financial ratios over the specified period. This study contributes to portfolio management by informing equity allocation in the short-term and long-term.

Investors are constantly searching for methods to generate value above passive investment techniques. Therefore, analysing the performance of hedge funds as compared to mutual funds, particularly in the wake of Covid-19, can aid investors in their investment decision-making process. Those investors who desire above-average returns, particularly in volatile market conditions place an expectation on hedge funds to be able to achieve higher performance during economic downturns, given that they are designed to mitigate risk and to take advantage of harsh financial market conditions. Monthly, secondary data were collected from 30 September 2018 to 31 August 2021 to analyse and compare the risk-adjusted performance of five hedge funds and five mutual funds in South Africa. Both hedge and mutual funds indicated higher risk-adjusted returns from the pre-Covid-19 period compared to during the pandemic. Hedge funds were found to have higher risk-adjusted returns than mutual funds during the Covid-19 period. The novelty of these results indicated that hedge fund managers can achieve higher returns for investors during extreme market events.

Background: Trail running is characterised by large elevation gains/losses and varying uneven running surfaces. Limited literature is available to help guide injury prevention strategies among trail runners. The purpose of this study was to determine the epidemiology, clinical characteristics, and related risk factors for running-related injuries (RRIs) amid trail runners who entered the 2021 Mac Mac Ultra races. Methods: Design: Descriptive cross-sectional study. Setting: 2021 Mac Mac Ultra Race. Participants: Consent for data analysis was given by 251 of 330 (76%) race entrants. Main outcome measures: Point prevalence (%), frequency (n, %), retrospective annual incidence (RRIs/100 athlete-years), characteristics (pathology type, tissue type, body area, anatomical region), and associated injury risk factors (training and demographic variables) of RRIs. Results: In the sample, the retrospective annual incidence was 19.92/100 athlete-years. The point prevalence was 4%. Injuries mostly appeared in the lower limb (95%), with the lower leg (26%), thigh (22%), ankle and foot (13%) described as the highest injured body areas. Of tissue type injuries, muscle/tendon comprised 60%. Muscle injury (36%), tendinopathy (24%), and joint sprain (9%) were the most reported pathology types. No related injury risk factors were discovered in this study. Conclusions: One in five trail runners reported one or more RRI during the 12 months before a competitive event. RRIs commonly involved the lower limb, especially the lower leg, thigh, foot, and ankle. More studies are needed to establish injury risk factors.

Temperature is considered to be a major abiotic factor influencing aquatic life. Marine heatwaves are emerging as threats to sustainable shellfish aquaculture, affecting the farming of New Zealand’s green-lipped mussel [Perna canaliculus (Gmelin, 1791)]. In this study, P. canaliculus were gradually exposed to high-temperature stress, mimicking a five-day marine heatwave event, to better understand the effects of heat stress on the metabolome of mussels. Following liquid chromatography-tandem mass spectrometry analyses of haemolymph samples, key sugar-based metabolites supported energy production via the glycolysis pathway and TCA cycle by 24 h and 48 h of heat stress. Anaerobic metabolism also fulfilled the role of energy production. Antioxidant molecules acted within thermally stressed mussels to mitigate oxidative stress. Purine metabolism supported tissue protection and energy replenishment. Pyrimidine metabolism supported the protection of nucleic acids and protein synthesis. Amino acids ensured balanced intracellular osmolality at 24 h and ammonia detoxification at 48 h. Altogether, this work provides evidence that P. canaliculus has the potential to adapt to heat stress up to 24 °C by regulating its energy metabolism, balancing nucleotide production, and implementing oxidative stress mechanisms over time. The data reported herein can also be used to evaluate the risks of heatwaves and improve mitigation strategies for aquaculture.

Simple Summary: Although total calcium (Ca) is still commonly used in commercial poultry diet formulation, it does not consider how much Ca the broilers can use on a biological level. This study aimed to measure how much Ca and phosphorus (P) from plant-based feed ingredients the broilers can digest, both with and without the addition of phytase. The goal of this study was to gather information to aid in the transition towards a system that uses digestible Ca and P for poultry diet formulation, improving accuracy, mineral utilization, and sustainability in global poultry farming. To begin formulating broiler diets on a digestible calcium (Ca) basis, robust Ca digestibility values for ingredients and factors affecting this digestibility are needed. This study had three main objectives: (1) determine the standardized ileal digestibility (SID) of Ca and phosphorus (P) for seven plant-based feed ingredients in broilers, (2) assess the impact of phytate source on SID Ca from limestone (LS), and (3) evaluate the effect of phytase on SID Ca and P for the different ingredients. Two experiments were conducted to satisfy these objectives. In Experiment 1, a 4 × 2 × 2 factorial design was used, with four plant-based feed ingredients (corn, wheat, sorghum, and full-fat soybean meal (FFS)), two LS inclusions in the diet (absence of LS and the inclusion of LS required to achieve 0.65% Ca in the final diet), and two phytase doses (0 and 1000 FTU/kg diet). Experiment 2 utilized a 3 × 2 × 2 factorial design with three plant-based ingredients (soybean meal (SBM), rapeseed meal (RSM), and sunflower meal (SFM)), two LS inclusions in the diet (absence of LS and the inclusion of LS required to achieve 0.65% Ca in the final diet), and two phytase doses (0 and 1000 FTU/kg diet). The trial had eight replicate pens (6 broilers/replicate) per treatment. Data were analyzed using a factorial analysis in JMP Pro 16.0 with means separation performed when p < 0.05, using Tukey HSD. The SID Ca in the absence of phytase for wheat (72.9%) and FFS (69.9%) was higher (p < 0.05) than for sorghum (54.5%) and corn (46.3%). In Experiment 2, the SID Ca in the absence of phytase from SFM (61.0%) was higher (p < 0.01) than RSM (42.7%) and SBM (46.8%). The SID Ca from added LS was affected by the ingredient, with diets containing wheat and FFS resulting in the lowest (p < 0.05) SID Ca versus those containing corn and sorghum irrespective of phytase dose in Experiment 1, and the lowest (p < 0.05) for SBM and RSM vs. SFM in the absence of phytase in Experiment 2. Phytase supplementation increased (p < 0.01) SID Ca and SID P across all feed ingredients compared to non-supplemented diets. There was a two-way interaction (p < 0.01) of LS addition and ingredient on SID P in both experiments. The results of this study provide SID Ca and SID P values from the selected ingredients and show that phytate from different ingredients reacts differently with Ca from LS and should be considered when developing SID coefficients of Ca and P for use in commercial broiler feed formulation. The SID coefficients of Ca and P for the individual feed ingredients evaluated in this study will allow for the further development and transition towards dCa and dP in commercial feed formulation. [ABSTRACT FROM AUTHOR]

Background: Despite the numerous health benefits of distance running, it is also associated with the development of 'gradual onset running-related injuries' (GORRIs) one of which is Iliotibial Band Syndrome (ITBS). Novel risk factors associated with a history of ITBS (hITBS) have not been described in a large cohort of distance runners. Objective: To identify risk factors associated with hITBS in distance runners. Design: Descriptive cross-sectional study. Setting: 21.1 km and 56 km Two Oceans Marathon races (2012–2015). Participants: 106 743 race entrants completed the online pre-race medical screening questionnaire. A total of 1 314 runners confirmed an accurate hITBS diagnosis. Methods: Selected risk factors associated with hITBS explored included: demographics (race distance, sex, age groups), training/running variables, history of existing chronic diseases (including a composite chronic disease score) and history of any allergy. Prevalence (%) and prevalence ratios (PR; 95% CI) are reported (uni- & multiple regression analyzes). Results: 1.63% entrants reported hITBS in a 12-month period. There was a higher (p < 0.0001) prevalence of hITBS in the longer race distance entrants (56 km), females, younger entrants, fewer years of recreational running (PR = 1.07; p = 0.0009) and faster average running speed (PR = 1.02; p = 0.0066). When adjusted for race distance, sex, age groups, a higher chronic disease composite score (PR = 2.38 times increased risk for every two additional chronic diseases; p < 0.0001) and a history of allergies (PR = 1.9; p < 0.0001) were independent risk factors associated with hITBS. Conclusion: Apart from female sex, younger age, fewer years of running and slower running speed, two novel independent risk factors associated with hITBS in distance runners are an increased number of chronic diseases and a history of allergies. Identifying athletes at higher risk for ITBS can guide healthcare professionals in their prevention and rehabilitation efforts. [ABSTRACT FROM AUTHOR]

The global rise in population has led to an increased demand for food production, necessitating the adoption of sustainable agricultural practices. Traditional methods often rely on synthetic chemicals that negatively impact both human health and the environment. This study aimed to screen soil fungal strains for plant-growth-promoting traits, specifically focusing on their ability to solubilize phosphates, produce indole-3-acetic acid (IAA), and synthesize siderophores. Fungal strains were identified using rDNA sequencing of the ITS regions, and their growth-promoting abilities were assessed in vitro. Aspergillus flavus JKJ7, Talaromyces purpureogenus JKJ12, and Trichoderma koningiopsis JKJ18 exhibited varying degrees of phosphate solubilization, with T. purpureogenus JKJ12 solubilizing the highest amount of tricalcium phosphate (TCP), while A. flavus JKJ7 was the most effective in solubilizing phytic acid calcium salt (PCS). In terms of IAA production, A. flavus JKJ7 produced the highest auxin concentration (68.51 mg/L), followed by T. koningiopsis JKJ18 and T. purpureogenus JKJ12. Additionally, A. flavus JKJ7 produced the highest amount of siderophores (83.7%), indicating its potential for improving iron uptake in plants. Principal Component Analysis (PCA) revealed distinct functional capabilities among the strains, particularly in phosphate solubilization and IAA production, suggesting their complementary use in consortium formulations. These results indicate that these fungal strains possess significant plant-growth-promoting traits and could be used as bioinoculants for sustainable agriculture, either as single strains or in combination [ABSTRACT FROM AUTHOR]

The purpose of this paper is to investigate the influence of cultural forces that shape social influence with a look at Hofstede’s cultural dimensions of power distance, uncertainty avoidance, and individualism. The direct effect of these cultural dimensions on social influence is investigated. To measure social influence, this paper relies on the dimensions of Hofstede. A multiple regression analysis is applied to answer the hypotheses. The research design adopted for the research was quantitative, and the method used to collect data was an online survey. A self-administered questionnaire was developed and distributed to the targeted respondents for obtaining primary data.The respondents involved were 340 SMEs in the urban area of Harare province in Zimbabwe. A nonprobability convenience sampling method was used for the study. Hofstede’s cultural factors of power distance, individualism, and uncertainty avoidance were used as the independent variables that moderate social influence. The results show that power distance significantly predicts uncertainty avoidance and individualism but does not directly influence social influence. However, uncertainty avoidance is a significant positive predictor of social influence. [ABSTRACT FROM AUTHOR]

The aim of this study was to investigate the physical characteristics of three litter materials, namely pine shavings (PS), bio-secure, fumigated pine shavings (BS) and sunflower hulls (SH) and its influence on broiler performance over a 33-day production cycle. The experiment was conducted in commercial poultry houses holding 42,500 chicks each, utilising a randomised block design with six house replicates per treatment. Litter samples were collected weekly for analyses of moisture, water-holding capacity, bulk density, pH and litter caking. Broiler footpad dermatitis was monitored at 21 and 31 days, together with acid detergent fibre (ADF) concentration of gizzard content, gizzard weight and small intestinal weight and length of 120 birds per treatment. Broilers across treatments consumed litter material which was evident in increased ADF levels of gizzard contents relative to feed. The SH contained more nutrients based on proximate analysis as compared to other treatments. Rearing on SH led to lower 7-day cumulative mortality, higher kilograms of broilers produced per square meter, average daily gain and slaughter weight. Improvements seen with SH did not alter commercial indicators, namely, production efficiency factor and feed conversion ratio. Litter converged toward similar physical characteristics at the end of production cycles when few differences were observed between treatments due to addition of feed, feathers and excreta.

Abstract Background It is plausible that optimal cardiovascular disease (CVD) risk management differs in patients with rheumatoid arthritis (RA) from low or middle income compared to high income populations. This study aimed at producing evidence-based points to consider for CVD prevention in South African RA patients. Methods Five rheumatologists, one cardiologist and one epidemiologist with experience in CVD risk management in RA patients, as well as two patient representatives, two health professionals and one radiologist, one rheumatology fellow and 11 rheumatologists that treat RA patients regularly contributed. Systematic literature searches were performed and the level of evidence was determined according to standard guidelines. Results Eighteen points to consider were formulated. These were grouped into 6 categories that comprised overall CVD risk assessment and management (n = 4), and specific interventions aimed at reducing CVD risk including RA control with disease modifying anti-rheumatic drugs, glucocorticoids and non-steroidal anti-inflammatory drugs (n = 3), lipid lowering agents (n = 8), antihypertensive drugs (n = 1), low dose aspirin (n = 1) and lifestyle modification (n = 1). Each point to consider differs partially or completely from recommendations previously reported for CVD risk management in RA patients from high income populations. Currently recommended CVD risk calculators do not reliably identify South African black RA patients with very high-risk atherosclerosis as represented by carotid artery plaque presence on ultrasound. Conclusions Our findings indicate that optimal cardiovascular risk management likely differs substantially in RA patients from low or middle income compared to high income populations. There is an urgent need for future multicentre longitudinal studies on CVD risk in black African patients with RA.

The production cost of cellulases is regarded as a limiting factor in the cellulosic ethanol production chain. Trichoderma and Aspergillus species were used to produce cellulases through solid-state fermentation (SSF) utilizing banana pseudostem (BPS) as a carbon source. The production of cellulases was investigated at various substrate moisture contents (65–80%), incubation temperatures (30–40 °C), substrate pre-treatment methods (3% w/v NaOH, 5% v/v H2SO4, and water), and with different co-culturing of microorganisms. Trichoderma longibrachiatum LMLSAUL 14-1 produced the maximum total cellulase (75 FPU/g d.s), endoglucanase (11.35 U/g d.s), and β-glucosidase (235.83 U/g d.s) activities at a 75% moisture content of the untreated BPS at 30 °C in static culture. Pre-treatment of BPS improved the production of specific enzymes. Aspergillus fumigatus LMLPS 13-4 produced more β-glucosidase (259.8 U/g d.s) when grown on acid-pre-treated BPS, whereas T. harzianum LMLBP07 13-5 produced the highest β-glucosidase activity (319.5 U/g d.s) on alkali-pre-treated BPS. Co-culturing of T. harzianum LMLBP07 13-5 and A. fumigatus LMLPS 13-4 improved the production of endoglucanase. These results suggest that banana pseudostem, a waste product of the banana industry, could be a potentially cheaper and abundant substrate for the production of the cellulase enzymes.

Neuronal cells can be generated from adipose-derived stem cells (ADSCs) through biological or chemical inducers. Research has shown that this process may be optimized by the introduction of laser irradiation in the form of photobiomodulation (PBM) to cells. This in vitro study is aimed at generating neuronal-like cells with inducers, chemical or biological, and at furthermore treating these transdifferentiating cells with consecutive PBM of a 525 nm green (G) laser and 825 nm near-infrared (NIR) laser light with a fluence of 10 J/cm2. Cells were exposed to induction type 1 (IT1): 3-isobutyl-1-methylxanthine (IBMX) (0.5 mM)+indomethacin (200 μM)+insulin (5 μg/ml) for 14 days, preinduced with β-mercaptoethanol (BME) (1 mM) for two days, and then incubated with IT2: β-hydroxyanisole (BHA) (100 μM)+retinoic acid (RA) (10-6 M)+epidermal growth factor (EGF) (10 ng/ml)+basic fibroblast growth factor (bFGF) (10 ng/ml) for 14 days and preinduced with β-mercaptoethanol (BME) (1 mM) for two days and then incubated with indomethacin (200 μM)+RA (1 μM)+forskolin (10 μM) for 14 days. The results were evaluated through morphological observations, viability, proliferation, and migration studies, 24 h, 48 h, and 7 days post-PBM. The protein detection of an early neuronal marker, neuron-specific enolase (NSE), and late, ciliary neurotrophic factor (CNTF), was determined with enzyme-linked immunosorbent assays (ELISAs). The genetic expression was also explored through real-time PCR. Results indicated differentiation in all experimental groups; however, cells that were preinduced showed higher proliferation and a higher differentiation rate than the group that was not preinduced. Within the preinduced groups, results indicated that cells treated with IT2 and consecutive PBM upregulated differentiation the most morphologically and physiologically.

Background: Child participation is critical for evaluation in the light of all Sustainable Development Goals (SDGs) and leaving no one behind. Resilience Analysis Consulting developed a child-friendly version (CFV) of the National Child Participation Framework (NCPF). To ensure participation and optimal use of the document, children were included in all aspects of the document development. Objectives: In this study children reflect on their experiences. This provides insight for document development and more widely into child participation in interventions for children and the evaluation of such interventions. It further highlights the value of child participation and encourages evaluators to include children. Methods: The children who assisted in the development of the document were asked to reflect on the process and their experiences through an open-ended survey. Scales were used to investigate the benefits. Open-ended questions were thematically analysed. Results: The experiences included the following main themes: (1) enjoyment of the project activities, (2) satisfaction with contributions, (3) learning new information, (4) being more aware of children’s rights and participation in society, (5) contributing to other children’s lives and (6) the importance of recognition and being valued. Seeing their ideas, artwork and recommendations in print made them feel that they were taken seriously. Conclusion: A participatory approach when developing materials for children enhances the uptake and dissemination of information. Children contribute unique and valuable perspectives to the evaluation process. Evaluators wrongly assume negative consequences, preventing them from including children, while children report positive outcomes. The process empowered child participants.

Background: The Global Evaluation Agenda 2020 calls for evaluation of Sustainable Development Goals (SDGs) within an equity-focused and gender-responsive lens. Most of the methodologies and materials come from the Global North. However, gender issues and evaluation capacity in the Global North do not necessarily match with those in the Global South. The Global South has rich experiences related to equity and gender. An important group to target to build capacity is young and emerging evaluators (YEEs). Objectives: This study investigated the gender responsive evaluation training experiences and needs of YEEs in Africa. Method: A total of 72 respondents completed an online survey that was administered over a 7-day period. The quantitative survey was self-administered in French and English, and was distributed through existing Voluntary Organisation for Professional Evaluation (VOPEs) and youth evaluators forums. Results: Respondents were from 23 African countries. Most of the respondents were YEEs, although many of the respondents did not self-identify as an YEE. One-third of respondents have participated in training programmes on gender responsive evaluation or a gender focus on evaluation. Virtual trainings have not been used. Topics included evaluating gender focussed interventions and gender responsive aspects of evaluation studies in general (including applying gender perspective to all types of policies, and participatory approaches to ensure gender equity). Conclusion: This study has practical value for training and mentoring of YEEs in gender focused evaluations in Africa. It will contribute to efforts of the South-to-South project and global efforts that ensure that ‘no one is left behind’ from a gender lens.

Background: Chronic exertional compartment syndrome (CECS) of the forearm is a rare but important cause of morbidity amongst athletes involved in strenuous upper limb activities. The diagnosis remains challenging due to the absence of objective, reproducible diagnostic studies. Objectives: To assess and quantify signal intensity (SI) changes of involved muscles in patients with CECS of the forearm compared to healthy control subjects competing in similar sporting disciplines. Also, to objectively measure MRI SIs within muscle compartments when using a pre- and post-exercise regime and calculating a signal intensity ratio (SIR) between post- and pre-exercise studies. Method: The study retrospectively examined MRI scans of patients treated for CECS of the forearm and compared these to the MRI scans of asymptomatic high-level rowers. A specific, reproducible pre- and post-exercise MRI scanning protocol was utilised in both patient and control subjects between 2011 and 2020. Signal intensities were evaluated pre- and post-exercise in involved muscle groups and ratios were calculated. Results: A total of 86 SIs were measured (43 pre- and 43 post-exercise) in nine study participants (five patients and four controls). After post:pre-exercise comparisons, a statistically significant difference was found between control and patient groups (p = 0.0010). The extensor carpi radialis, flexor digitorum profundus and flexor digitorum superficialis muscles were most commonly involved. Conclusion: This study confirms that significant SI changes are apparent in patients with CECS of the forearm when making use of a standardised pre- and post-exercise MRI protocol. Furthermore, SIR may be used to accurately diagnose CECS of the forearm.

Cultivar development of cowpea with increased grain yield depends on evaluation and selection of genetically unique and complementary breeding lines. The objective of this study was to evaluate cowpea collections using agronomic traits to select promising parents to develop breeding population. Twenty-two cowpea genotypes were field evaluated using a randomised complete block design with three replications in two locations in South Africa. Analysis of variance revealed significant genotypic (G) and locations (L) differences for all agronomic traits evaluated. Significant differences were also observed for most of the traits due to G X L, G X Year (Y), L X Y and G X L X Y interactions effects. The genotypes tested at two locations showed considerable genetic variation for the following agronomic traits that varied for number of leaves (NL) (20.08–179.78), leaf length (LL) (12.36–67.72 cm), leaf width (LW) (4.78–22.02 cm), number of branches (NB) (6.81–13.85), number of pods per plant (NPPP) (14.33–54.04), number of seeds per pod (NSPP) (11.7286.28), pod diameter (PD) (5.75–22.73 mm), plant height (PH) (6.57–233.71 cm), pod length (PL) (3.94–100.92 mm), hundred seed weight (HSWt) (8.11–49.52 g) and grain yield (GY) (0.10-1.48 t ha−1). Grain yield was significant and positively correlated with all of the traits except leaf length. The principal component analysis (PCA) identified four principal components (PCs) contributing to 73.62% of the total explained variation amongst the tested genotypes. The selected cowpea genotypes for most of agronomic characteristics are useful and candidate genetic resources for the development of breeding population in cowpea breeding and enhanced production and productivity for food and nutrition in the country.

Taro [Colocasia esculenta (L.) Schott] is an important underutilised staple food crop in South Africa, with a lot of potential to address food insecurity among poor rural households. Development of high yielding stable taro cultivars is one of the most important goals of plant breeders. Twenty-nine taro accessions collected from major taro producing regions of the country were evaluated for growth performance, yield potential and stability under dryland conditions at two sites (Umbumbulu and Roodeplaat) in 2013, 2014 and 2015 cropping seasons. The experiment was laid in a randomised complete block design replicated three times. Growth and yield traits were measured. Analysis of variance and correlation analysis was done on all measured traits. The genotype by environment interaction was analysed using additive main effects and multiplicative interaction (AMMI). As a result, significant variation was observed for most of the traits except number of leaves and leaf width as well as number of suckers, while all the traits showed significant variation for location by year interaction. Number of corms showed significant variation for location by year by genotype interaction among all the traits evaluated. Genotype effect was highly significant (p

Origanum marjorana L. has been valued for centuries for its flavoring attributes and therapeutic properties. The growing demand for its various applications necessitates optimizing agronomic practices for its production. A glasshouse pot trial was conducted to identify optimum agronomic practices for increased herbage and oil yield, as well as oil quality. The effects of varying air temperature regimes (low, medium, and high levels), irrigation (low, medium, and high levels), nitrogen fertilizer application (N = 100, 150, and 200 kg/ha), and soil type (sandy loam, sandy clay loam, and loamy sand) on the productivity of marjoram plants were investigated. The results showed an increase in plant growth and herbage yield as well as chlorophyll content under conditions of high air temperature, low irrigation, and moderate to high nitrogen level applied to sandy loam soil, with an increase in oil yield with loamy sand soil. The major compounds observed in marjoram essential oil were terpinene-4-ol (22.63–36.72%) and (Z)-β-terpineol (6.85–16.60%), in which terpinene-4-ol was not found to be within the International Organization for Standardization (ISO) limits of acceptability while (Z)-β- terpineol had no reference limits available. A promising performance of marjoram cultivation under high regimes of air temperature (16.7 to 36.6 °C), nitrogen fertilization (200 kg ha−1 N), and low irrigation (up to 60% soil water depletion from field capacity) on sandy loam soils was demonstrated for improved crop productivity.

Orientation: Although significant progress has been made globally in gender equality, women still occupy less political influence, fewer leadership positions and yield less control over their careers than most men. Gender inequality is evident in male-dominated work environments such as the security industry. Research purpose: This study reflects on women’s perceptions on gender equality and career progression in the South African security industry. Motivation for the study: In post-democracy South Africa, women are categorised as previously disadvantaged, therefore a priority group in terms of advancement. However, it is still unclear, from the narratives of the women themselves, how their career progression is encumbered in the milieu of the security industry. Research approach/design and method: Through qualitative one-on-one semi-structured interviews, 15 women, working in the security industry, shared their experiences concerning gender equality and career progression. Data were analysed thematically, guided by the context of the gendered security profession. Main findings: The findings reveal that women experience slower career progression than men in terms of rejection and work allocation. Moreover, negative perceptions of female leadership among colleagues was a factor hindering career progression. Practical/managerial implications: This study argues by doing gender well, equality in the security workplace can be obtained. Furthermore, the study encourages South African security managers to recognise how aspects such as rejection, work allocation and a negative perception of female leadership may encumber the career progression of female security professionals. Contribution/value-add: The study contributes to scientific knowledge and discourse regarding women’s perceptions on gender equality and career progression.

Taro [Colocasia esculenta (L.) Schott] has the potential to address food and nutrition insecurity in sub-Saharan Africa. However, the nutrient content of taro is yet to be fully elucidated. The objective of this study was to evaluate mineral element content as a proxy for nutritional value of different taro genotypes. The study evaluated 14 taro accessions at Roodeplaat and Umbumbulu in South Africa based on their calcium (Ca), iron (Fe), potassium (K), magnesium (Mg), manganese (Mn), sodium (Na), phosphorous (P) and zinc (Zn) content. The accessions were planted in a randomized complete block design, replicated three times under field conditions. The mineral element content varied significantly (p < 0.05) among the genotypes. Genotypes Amad7-2, Umbu8 and Amad101 exhibited high Ca (≥432 mg kg−1), Fe (≥32 mg kg−1) and Mg (≥229 mg kg−1) across the locations. The first principal component (PC) accounted for 33.7% of the variation and was strongly associated with Zn (r = 0.94, p < 0.001) and P (r = 0.89, p < 0.001). The second PC explained 29.7% of the variation and was associated with Na (r = 0.83, p < 0.001), Mg (r = 0.76, p < 0.001) and K (r = 0.55, p < 0.05). Fe and Mn contributed below the 12.5% threshold to the PCs and were considered as less discriminatory among the accessions. The negative correlations among some of the mineral elements would be a challenge for selection and breeding of nutritious taro accessions. This information is essential to select superior local accessions based on their mineral element content for developing breeding populations and lines for improving nutrition quality among poor households in sub-Saharan Africa.

Banana pseudostem (BPS) is an agricultural waste with a high holocellulose content, which, upon hydrolysis, releases fermentable sugars that can be used for bioethanol production. Different pretreatment methods, namely, 3% (w/v) NaOH, 5% (v/v) H2SO4, and liquid hot water, applied on the BPS resulted in the availability of 52%, 48%, and 25% cellulose after treatment, respectively. Saccharification of the pretreated BPS with 10 FPU/g dry solids (29.3 mg protein/g d.s) crude enzyme from Trichoderma harzianum LMLBP07 13-5 at 50°C and a substrate loading of 10 to 15% released 3.8 to 21.8 g/L and from T. longibrachiatum LMLSAUL 14-1 released 5.4 to 43.5 g/L glucose to the biomass. Ethanol was produced through separate hydrolysis and fermentation (SHF) of alkaline pretreated BPS hydrolysate using Saccharomyces cerevisiae UL01 at 30°C and 100 rpm. Highest ethanol produced was 17.6 g/L. Banana pseudostem was shown as a potentially cheap substrate for bioethanol production.

Grain legumes, including cowpea, are the cheapest sources of minerals and protein to enhance human nutrition. Cultivar development and deployment of cowpea with increased grain mineral content and protein composition rely on selection of genetically unique and complementary breeding lines. The objective of this study was to assess the grain minerals and protein composition of diverse cowpea collections of eastern, southern and western Africa to select promising parents to develop a breeding population. Twenty-two genetically diverse cowpea genotypes were field evaluated using a randomised complete block design with three replications in two locations in South Africa. The dried grain mineral contents were determined using an Atomic Absorption Spectrophotometre, while the protein content was determined by the combustion method. Analyses of variance showed significant (P

Skeletal muscle relaxants (SMRs) consist of a heterogeneous group of medications with a side effect profile of concern. The aim of this paper was to review the evidence of use of SMRs in the treatment of sports injuries. A literature search between 2005 – 2018 (Ovid MEDLINE, SPORTDiscus and SCOPUS) were conducted. In addition, citations within articles were searched, and the most commonly prescribed SMRs in South Africa were also used to explore the literature for additional publications. Relevant studies that met the inclusion criteria were selected. Clinical recommendations for general practitioners are given based on the Strength of Recommendation Taxonomy (SORT) level of evidence. Combination drugs rather than single agents are mostly used, however the effectiveness of SMR agents, single and in combination, as well as its significance as opposed to analgesics and non-steroidal anti-inflammatory drugs, still has to be evaluated. Evidence suggest SMRs to be probably effective for use in non-specific lower back pain (acute and chronic lower back muscle strains, ligament sprains, soft tissue contusions), as well as for whiplash associated disorder, mechanical neck disorders, piriformis syndrome, lateral epicondylosis, and plantar fasciitis. It does not appear if there is a role for SMRs as part of combination management for acute cervical strains, post-exercise muscle soreness or myofacial pain syndrome. However, substantial evidence to confirm the use of SMRs in the treatment of sports injuries have not been adequately investigated and is currently largely based on case reports and general reviews.

The objective of this study was to determine the dietary protein and apparent metabolisable energy (AME) requirements of local chickens. Freshly laid eggs of scavenging chickens collected in rural villages were hatched and randomly distributed to 27 floor pens, 10 chicks per pen. Chicks were fed 9 experimental diets that were combinations of three CP levels (140, 170 and 190 g kg−1 DM) and three AME levels (11.0, 11.7 and 12 MJ kg−1) during the starter phase (0–6 weeks) and combinations of three CP levels (120, 150 and 180 g kg−1 DM) and three ME levels (11.3, 12.0 and 12.4 MJ kg−1) during the grower phase (7–17) weeks. Significant differences within means on CP × AME interaction effect were observed in all parameters measured, except feed intake during starter period and dressing percentage (%) and breast yield of 17 weeks old chickens. The results of the present study indicated that during the starter and grower phases, unsexed chickens would require dietary combinations of 170 g CP kg−1 and 11.0 AME MJ kg−1 and 150 g CP kg−1 and 12 AME MJ kg−1 in their diets to optimise weight gain and FCR, and 150 g CP kg−1 and 11.3 MJ kg−1 to optimise ash content of muscles, protein content of the breast and fat content of the leg muscle. Supplementation of 27 g CP kg−1 feed to grower scavenging chickens would be enough to improve chicken production in the rural villages.