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The aim of this paper is contribution to understanding of coupling between the polarization modes on the resultant transfer properties of single mode fiber given by the Jones matrix. In the case of ideal fiber it is possible to describe a propagation of the polarized optical wave by the Jones matrix and corresponding polarization eigenmodes with different propagation described by the Jones matrix are given not by the eigenmodes but by the principal states of polarization. Defined disturbance by the Jones matrix are given not by the eigenmodes but by the principal states of polarization. Defined disturbance along the whole fiber and also relatively defined variable coupling between the polarization modes it is possible to obtain by the torsion of fiber. Experimental evaluation of torsion effect by the Jones matrix elements can define the boundaries of application theory of polarization eigenmodes and principal states of polarization. These results can also contribute to understanding of conditions for polarization preserving of fiber and the development of polarization dispersion during disturbance of propagation conditions. Jones matrix elements have been measured on the working place published before and calculation and optimization of result are done by the use of software made for this purpose with the application of quaternions.

The infection of woodchucks with woodchuck hepatitis virus (WHV) provides an experimental model to study early immune responses during hepadnavirus infection that cannot be tested in patients. The T-cell response of experimentally WHV-infected woodchucks to WHsAg, rWHcAg, and WHcAg peptides was monitored by observing 5-bromo-2′-deoxyuridine and [2- 3 H]adenine incorporation. The first T-cell responses were directed against WHsAg 3 weeks after infection; these were followed by responses to rWHcAg including the immunodominant T-cell epitope of WHcAg (amino acids 97 to 110). Maximal proliferative responses were detected when the animals seroconvered to anti-WHs and anti-WHc (week 6). A decrease in the T-cell response to viral antigens coincided with clearance of viral DNA. Polyclonal rWHcAg-specific T-cell lines were established 6, 12, 18, and 24 weeks postinfection, and their responses to WHcAg peptides were assessed. Five to seven peptides including the immunodominant epitope were recognized throughout the observation period (6 months). At 12 months after infection, T-cell responses to antigens and peptides were not detected. Reactivation of T-cell responses to viral antigens and peptides occurred within 7 days after challenge of animals with WHV. These results demonstrate that a fast and vigorous T-cell response to WHsAg, rWHcAg, and amino acids 97 to 110 of the WHcAg occurs within 3 weeks after WHV infection. The peak of this response was associated with viral clearance and may be crucial for recovery from infection. One year after infection, no proliferation of T cells in response to antigens was observed; however, the WHV-specific T-cell response was reactivated after challenge of woodchucks with WHV and may be responsible for protection against WHV reinfection.