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本研究の目的は，保育内容（健康）に関わるクロスロードの問題作成および実践の結果を通して，保育者が子ども達に保育内容の健康領域の指導法について考察することである．保育者養成機関の学生にクロスロード問題の作問および実践を行った．その結果，保育内容の健康領域に関するクロスロード問題が６問作成された．その内容は，生活習慣に関すること，運動遊びに関すること，安全に関すること，そして，感染症予防に関することと多岐にわたっていた．また，実践を行うと，回答の比率割合が，5：5になる問題が2問，そして，1：9になる問題が1問であった．以上のことから，保育内容の健康領域に関する指導について，クロスロードの作問と実践を行うことにより，保育に関する様々な役割の立場に立って課題について考えることを可能にする．そして，子ども達を指導する際に必要となる想像力を育むうえでも効果的であると考えられる．

The supplementary document contains the information that might be necessary for completion and would facilitate the reproducibility of results.

The document contains all simulation and experimental datasets generated during the titled study.

Left-right (L-R) asymmetry in the body plan is determined by nodal flow in vertebrate embryos. Shinohara et al. (Shinohara K et al. 2012 Nat. Commun. 3, 622 (doi:10.1038/ncomms1624)) used Dpcd and Rfx3 mutant mouse embryos and showed that only a few cilia were sufficient to achieve L-R asymmetry. However, the mechanism underlying the breaking of symmetry by such weak ciliary flow is unclear. Flow-mediated signals associated with the L-R asymmetric organogenesis have not been clarified, and two different hypotheses-vesicle transport and mechanosensing-are now debated in the research field of developmental biology. In this study, we developed a computational model of the node system reported by Shinohara et al. and examined the feasibilities of the two hypotheses with a small number of cilia. With the small number of rotating cilia, flow was induced locally and global strong flow was not observed in the node. Particles were then effectively transported only when they were close to the cilia, and particle transport was strongly dependent on the ciliary positions. Although the maximum wall shear rate was also influenced by ciliary position, the mean wall shear rate at the perinodal wall increased monotonically with the number of cilia. We also investigated the membrane tension of immotile cilia, which is relevant to the regulation of mechanotransduction. The results indicated that tension of about 0.1 mu Nm(-1) was exerted at the base even when the fluid shear rate was applied at about 0.1 s(-1). The area of high tension was also localized at the upstream side, and negative tension appeared at the downstream side. Such localization may be useful to sense the flow direction at the periphery, as time-averaged anticlockwise circulation was induced in the node by rotation of a few cilia. Our numerical results support the mechanosensing hypothesis, and we expect that our study will stimulate further experimental investigations of mechanotransduction in the near future.

It contains the data for underlying figures collected during the course of the research for the journal paper titled as 'The bubble induced population dynamics of fermenting yeasts'.

It contains text, figures and references that fall outside the main document but is required to facilitate understanding.

It contains the data for underlying figures as well as the source code developed during the course of the research for the journal paper titled as 'On bubble induced population dynamics of fermenting yeasts'.

This data set contains revised set of data for the figures and graphs in the titled paper.