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559 results on '"Ishihara, Kazuyuki"'

51. Cloning and expression of the aspartate carbamoyltransferase gene from Treponema denticola

Author

Ishihara, Kazuyuki, Ishihara, Michiyo, Takazoe, Ichiro, and Okuda, Katsuji

Subjects
Periodontal disease -- Research, Transferases -- Genetic aspects, Biological sciences
Abstract

An investigation was conducted to clone and characterize antigenic proteins of Treponema denticola, an oral spirochete associated with the subgingival plaque in periodontitis. One clone chosen for further analysis coded for a protein with 33.8% homology to the aspartate carbamoyltransferase (ATCase) of Escherichia coli. The protein-coding region was isolated and expressed in E. coli, and the expressed product was partially purified. Enzyme assays on the partially purified product revealed the presence of ATCase activity. Furthermore, the protein reacted with antisera against T. denticola, demonstrating its antigenicity.

Published
1992

53. Effect of smoking on subgingival microflora of patients with periodontitis in Japan

Author

Yamada Satoru, Tanno-Nakanishi Mariko, Kubota Michiya, Okuda Katsuji, and Ishihara Kazuyuki

Subjects
Dentistry, RK1-715
Abstract

Abstract Background Smoking is a risk factor for periodontitis. To clarify the contribution of smoking to periodontitis, it is essential to assess the relationship between smoking and the subgingival microflora. The aim of this study was to gain an insight into the influence of smoking on the microflora of Japanese patients with periodontitis. Methods Sixty-seven Japanese patients with chronic periodontitis (19 to 83 years old, 23 women and 44 men) were enrolled in the present study. They consisted of 30 smokers and 37 non-smokers. Periodontal parameters including probing pocket depth (PPD) and bleeding on probing (BOP) and oral hygiene status were recorded. Detection of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Fusobacterium nucleatum/periodonticum, Treponema denticola and Campylobacter rectus in subgingival plaque samples was performed by polymerase chain reaction. Association between the detection of periodontopathic bacteria and smoking status was analyzed by multiple logistic regression analysis and chi-square test. Results A statistically significant association was found between having a PPD ≥ 4 mm and detection of T. denticola, P. intermedia, T. forsythia, or C. rectus, with odds ratios ranging from 2.17 to 3.54. A significant association was noted between BOP and the detection of C. rectus or P. intermedia, and smoking, with odds ratios ranging from 1.99 to 5.62. Prevalence of C. rectus was higher in smokers than non-smokers, whereas that of A. actinomycetemcomitans was lower in smokers. Conclusions Within limits, the analysis of the subgingival microbial flora in smokers and non-smokers with chronic periodontitis suggests a relevant association between smoking and colonization by the specific periodontal pathogens including C. rectus.

Published
2011
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55. Clinical guidelines for the early detection of plantar malignant melanoma

Author

Saida, Toshiaki, Yoshida, Nagako, Ikegawa, Shuichi, Ishihara, Kazuyuki, and Nakajima, Takashi

Subjects
Mole (Dermatology) -- Measurement, Melanoma -- Diagnosis, Health
Abstract

Early detection of malignant melanoma, a cancer with a poor prognosis characterized by pigmented moles or tumors, is essential in combatting the disease. The most common site for malignant melanoma to arise in nonwhites is the palm of the hand and the region under the nails (subungual region). Melanoma on the plantar surface (sole) of the foot tends to be diagnosed only when advanced. To aid in early detection of such malignancies, a study of 144 pigmented lesions removed from the soles of the feet of 137 Japanese subjects was performed. Histologic diagnoses were divided into six categories. These were: congenital melanocytic nevus (mole composed of melanocytes, the cells that form pigment, present since birth); ordinary acquired melanocytic nevus; dysplastic nevus, a type of benign acquired melanocytic nevus; possible lesion of malignant melanoma in situ (signs of malignant melanoma, but no definite diagnosis possible); malignant melanoma in situ; and malignant melanoma. Ninety-three melanocytic nevi and 51 malignant melanomas were identified. The 48 invasive melanomas were further categorized. No melanoma, including the seven that were in situ, was less than nine millimeters in maximum diameter, and the smallest invasive specimen was 11 millimeters in diameter. Patients with benign melanocytic lesions tended to be younger than age 50, and most patients older than 50 had a malignant melanoma. The implications of these findings for public health screening are that any pigmented lesion on the sole of the foot that is larger than seven millimeters in diameter should be examined histologically. (Consumer Summary produced by Reliance Medical Information, Inc.)

Published
1990

60. Effect ofPorphyromonas gingivalisinfection in the placenta and umbilical cord in pregnant mice with low birth weight

Author

Udagawa, Sayuri, primary, Katagiri, Sayaka, additional, Maekawa, Shogo, additional, Takeuchi, Yasuo, additional, Komazaki, Rina, additional, Ohtsu, Anri, additional, Sasaki, Naoki, additional, Shiba, Takahiko, additional, Watanabe, Kazuki, additional, Ishihara, Kazuyuki, additional, Sato, Noriko, additional, Miyasaka, Naoyuki, additional, and Izumi, Yuichi, additional

Published
2018
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62. Transmission of Periodontal Disease-Associated Bacteria from Teeth to Osseointegrated Implant Regions.

Author

Sumida, Shinichi, Ishihara, Kazuyuki, Kishi, Masataka, and Okuda, Katsuji

Subjects
PERIODONTAL disease, BACTERIA, OSSEOINTEGRATED dental implants, PERIODONTAL pockets, POLYMERASE chain reaction, ORAL microbiology, INFECTIOUS disease transmission
Abstract

Purpose: The presence of periodontopathic bacteria is a risk factor for peri-implantitis. The present study examined colonization by periodontopathic bacteria and their transmission from periodontal pockets to osseointegrated implant sulcus. Materials and Methods: Plaque samples were collected from 105 sites in the 15 patients who participated in the study. Colonization by these bacteria was examined by polymerase chain reaction (PCR) and culture. The transmission of periodontopathic bacteria from periodontal sites of natural teeth to the implant sulcus was analyzed by pulsed field gel electrophoresis (PFGE). Results: The PCR detection rates of Porphyromonas gingivalis, Prevotella intermedia, Actinobacillus actinomycetemcomitans, Bacteroides forsythus, and Treponema denticola were 80.0%, 53.3%, 46.7%, 60.0% and 40.0%, respectively. Colonizations by P gingivalis and A actinomycetemcomitans were statistically correlated with periodontal pockets and implant sulcus regions (P < .01). The PFGE patterns of the P gingivalis strains isolated from each patient were identical, but differed from those from other patients. The PFGE patterns of P intermedia strains were identical in 2 out of 3 patients. Discussion: These analyses indicated that there appeared to be transmission of P gingivalis and P intermedia from the periodontal pocket to the peri-implant region. Conclusion: Elimination of these periodontal pathogens from the patient's oral cavity before administering dental implant treatment may inhibit colonization by these pathogens and reduce the risk of peri-implantitis. [ABSTRACT FROM AUTHOR]

Published
2002

65. Elevation of pro-inflammatory cytokine levels following anti-resorptive drug treatment is required for osteonecrosis development in infectious osteomyelitis

Author

Morita, Mayu, primary, Iwasaki, Ryotaro, additional, Sato, Yuiko, additional, Kobayashi, Tami, additional, Watanabe, Ryuichi, additional, Oike, Takatsugu, additional, Nakamura, Satoshi, additional, Keneko, Yosuke, additional, Miyamoto, Kana, additional, Ishihara, Kazuyuki, additional, Iwakura, Yoichiro, additional, Ishii, Ken, additional, Matsumoto, Morio, additional, Nakamura, Masaya, additional, Kawana, Hiromasa, additional, Nakagawa, Taneaki, additional, and Miyamoto, Takeshi, additional

Published
2017
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73. Effect of Porphyromonas gingivalis infection in the placenta and umbilical cord in pregnant mice with low birth weight.

Author

Udagawa, Sayuri, Katagiri, Sayaka, Maekawa, Shogo, Takeuchi, Yasuo, Komazaki, Rina, Ohtsu, Anri, Sasaki, Naoki, Shiba, Takahiko, Watanabe, Kazuki, Ishihara, Kazuyuki, Sato, Noriko, Miyasaka, Naoyuki, and Izumi, Yuichi

Subjects
PERIODONTITIS, LOW birth weight, PORPHYROMONAS gingivalis, UMBILICAL cord, ABNORMALITIES in mice, THERAPEUTICS, ANIMAL experimentation, BIRTH weight, INFLAMMATION, MICE, PLACENTA, TUMOR necrosis factors, GRAM-negative anaerobic bacteria
Abstract

Objective: Growing evidence indicates an association between periodontitis and delivery outcome; however, the mechanism is unclear. This study aimed to investigate the influence of Porphyromonas gingivalis (Pg) infection on delivery outcome in mice.Materials and Methods: Bacteremia was induced in pregnant Slc:ICR mice (8 weeks old) by intravenous injection of Pg. Mice were randomly divided into a control group (CO), and those receiving Pg injection at gestational day 1 (GD1), gestational day 15 (GD15) or every day (ED). Delivery outcome, Pg infection, and gene expression in the placenta and umbilical cord were evaluated.Results: Birth weight was lower in the ED and GD15 groups than in the CO group. A remarkable increase in anti-Pg IgG antibody was observed in the ED and GD1 groups, although Pg was not detected in the placenta or umbilical cord. mRNA expression of Tnfα and Il6 in the placenta, and Hif1α in the umbilical cord, was significantly increased in the ED group. Microarray analysis of the umbilical cord revealed increased expression of several genes including Orm1, Mgl2, Rps6ka3 and Trim15 in the ED group.Conclusions: Pg infection during the third trimester caused low birth weight and inflammation in the placenta and umbilical cord. [ABSTRACT FROM AUTHOR]

Published
2018
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74. Identification of a specific domain of <italic>Porphyromonas gingivalis</italic> Hgp44 responsible for adhesion to <italic>Treponema denticola</italic>.

Author

Yoshikawa, Kouki, Kikuchi, Yuichiro, Kokubu, Eitoyo, Imamura, Kentaro, Saito, Atsushi, and Ishihara, Kazuyuki

Subjects
PORPHYROMONAS gingivalis, MICROBIAL adhesion, TREPONEMA denticola, BIOFILMS, NUCLEOTIDE sequence, PATHOGENIC microorganisms
Abstract

Interaction between two periodontal pathogens, Porphyromonas gingivalis and Treponema denticola, contributes to plaque biofilm formation. Porphyromonas gingivalis forms aggregates with T. denticola through its adhesion/hemagglutinin domain (Hgp44). In this study, we investigated the specific domain of P. gingivalis Hgp44 responsible for adhesion to T. denticola using expression vectors harboring P. gingivalis Hgp44 DNA sequences encoding amino acid residues 1–419. Six plasmids harboring fragments in this region were generated by PCR amplification and self-ligation, and recombinant proteins r-Hgp44 (residues 1–419), r-Hgp441 (residues 1–124), r-Hgp442 (1–199), r-Hgp443 (1–316), r-Hgp444 (199–419), r-Hgp445 (124–198) and r-Hgp446 (199–316) were produced, as confirmed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and immunoblotting. r-Hgp44, r-Hgp443 and r-Hgp446 showed greater adhesion to T. denticola sonicates than the control, as determined by enzyme-linked immunosorbent assay. r-Hgp446 reduced the coaggregation of P. gingivalis and T. denticola. Scanning electron and confocal laser scanning microscopy analyses revealed that r-Hgp446 reduced dual-species biofilm formation. Our results indicate that residues 199–316 of P. gingivalis Hgp44 are mainly responsible for adhesion to T. denticola; inhibiting this domain could potentially disrupt periodontopathic biofilm formation and maturation. Porphyromonas gingivalis Hgp44 residues 199–316 are responsible for adhesion to Treponema denticola; inhibition of this domain may disrupt periodontopathic biofilm formation and maturation. [ABSTRACT FROM AUTHOR]

Published
2018
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77. Role of extracytoplasmic function sigma factors in biofilm formation of Porphyromonas gingivalis

Author

Onozawa, Satosu, primary, Kikuchi, Yuichiro, additional, Shibayama, Kazuko, additional, Kokubu, Eitoyo, additional, Nakayama, Masaaki, additional, Inoue, Tetsuyoshi, additional, Nakano, Keisuke, additional, Shibata, Yukinaga, additional, Ohara, Naoya, additional, Nakayama, Koji, additional, Ishihara, Kazuyuki, additional, Kawakami, Toshiyuki, additional, and Hasegawa, Hiromasa, additional

Published
2015
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81. Infection with Porphyromonas gingivalis Exacerbates Endothelial Injury in Obese Mice

Author

Ao, Min, primary, Miyauchi, Mutsumi, additional, Inubushi, Toshihiro, additional, Kitagawa, Masae, additional, Furusho, Hisako, additional, Ando, Toshinori, additional, Ayuningtyas, Nurina Febriyanti, additional, Nagasaki, Atsuhiro, additional, Ishihara, Kazuyuki, additional, Tahara, Hidetoshi, additional, Kozai, Katsuyuki, additional, and Takata, Takashi, additional

Published
2014
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83. Vinculin and Rab5 Complex Is Requited for Uptake of Staphyrococcus aureus and Interleukin-6 Expression

Author

Hagiwara, Makoto, primary, Kokubu, Eitoyo, additional, Sugiura, Shinsuke, additional, Komatsu, Toshinori, additional, Tada, Hiroyuki, additional, Isoda, Ryutaro, additional, Tanigawa, Naomi, additional, Kato, Yoshiko, additional, Ishida, Naoyuki, additional, Kobayashi, Kaoru, additional, Nakashima, Misako, additional, Ishihara, Kazuyuki, additional, and Matsushita, Kenji, additional

Published
2014
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