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Background: Triple negative breast cancer (TNBC) is well known for its more aggressive course and poorer prognosis compared to other BC subtypes. RegistEM study provides real world data to understand the distribution of BC subtypes in the advanced setting, being its primary objective. Biological samples collection is part of its procedures. This is a non-interventional cohort study and 1,907 patients (pts) have been enrolled up to now (females and males) with advanced BC (ABC), diagnosed from Jan-2016 to Dec-2019, either after recurrence or as first BC diagnosis, in 38 Spanish sites. These pts will be followed for at least 5 years. Methods: In the current analysis (cut-off date 08/April/2022, database ongoing), we describe characteristics, treatment patterns and outcomes, including comparison between recurrent and de novo disease, of 157 pts with advanced TNBC included in the RegistEM study. Those pts represent the 10% of pts available in the database at the cut-off date and with ABC diagnosis up to December 2018 (n=1559). The BC clinical subtypes were histologically confirmed on the most recent tumor lesion (metastatic [M] or primary BC) before starting with the 1st-line therapy. Results: At first ABC diagnosis, 73% pts had recurrent early BC (EBC), 26% de novo MBC and 1% unresectable locally ABC (ULABC). Median age was 57 years (range 30-88), all pts were women, 98% Caucasian and 65% postmenopausal. Family history of BC and/or ovarian cancer was reported in 37% pts, and a hereditary-risk genetic test was performed in 59 of 147 pts. Germline BRCA1/2 and TP53 were the most frequently mutated genes, 21% (6/28) and 47% (8/17) pts, respectively. Visceral involvement was present in 69% pts (similar between recurrent EBC and de novo ABC, although brain metastases were only present in the recurrent EBC group), and ≤ 2 metastatic locations in 59%. In 61% (70/115) pts with recurrent EBC, the subtype was assessed in metastatic lesions, and 39 pts of them also had TN subtype in primary BC. In terms of the most frequent therapies by line: 1) 1st-line: chemotherapy (CT) (60%) and CT/biological therapy (BT) (39%). Of the 87 pts with CT alone, monotherapy was the preferred option in 57% pts (capecitabine 25%, taxanes 16%, and eribulin or vinorelbine, 5% each). Bevacizumab was the most frequent BT (79%) combined with CT (single agent in 56% pts, mostly taxanes and capecitabine). Progressive disease (PD) was reported in 85% pts (similar in pts with both recurrent and de novo MBC or ULABC); 2) 2nd-line: CT (79%) (monotherapy capecitabine, eribulin, taxanes) and CT/BT (17%) (CT-containing bevacizumab 82%). Progression was reported in 92% pts; 3) 3rd-line: CT (90%) (eribulin 33%, platinum-based 25%) and CT/BT (9%) (CT-containing bevacizumab 67%). Progression was reported in 88% pts. At database cut-off date, death was reported in 133 (85%) pts, mainly because of PD. Overall survival (OS) was similar between both groups, recurrent and de novo MBC. Conclusion: In this population of Spanish TNBC pts with ABC, three quarters had recurrent disease. De novo ABC pts had a higher proportion of non-visceral metastases, with absence of brain involvement at the first diagnosis. Single-agent CT and CT plus bevacizumab were the most frequent therapies, and OS was similar between recurrent and de novo MBC pts, although numerically higher in the later group. Citation Format: Silvia Antolin Novoa, César A Rodríguez, Josefina Cruz, Sara López-Tarruella, Ariadna Tibau, Encarna Adrover, Ana Miguel, Mireia Margelí, Purificación Martínez, María Hernández, Antonio Antón, Álvaro Rodríguez-Lescure, Catalina Falo, Isabel Álvarez, Diego Malón, Raquel Andrés, José L Alonso-Romero, César Gómez, J. José Illaramendi, Ruth Campo, Juan José Miralles, Susana Bezares, Federico Rojo, Angel Guerrero-Zotano. Treatment strategies for advanced triple negative breast cancer patients as per routine clinical practice: analysis from the observational study GEICAM/2014-03 (RegistEM) [abstract]. In: Proceedings of the 2022 San Antonio Breast Cancer Symposium; 2022 Dec 6-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2023;83(5 Suppl):Abstract nr P4-07-45.

Background: HER2-low breast cancer (BC) is a new therapeutic entity, but it is uncertain if this subgroup of BC differs from HER2-negative tumors in clinical characteristics, prognosis, and response to therapy. The objective is to describe the clinical characteristics and outcomes of 422 patients (pts) with HER2-low BC documented before 1st line, having been diagnosed with advanced BC (ABC) before 2019 and included in the RegistEM study (n=1,663). Methods: In this analysis (cut-off date 08/April/2022; database is ongoing), two subgroups of pts have been considered based on HER2 results: HER2-low (n=422) (immunohistochemistry [IHC] 1+ or IHC 2+ and in situ hybridization [ISH] negative) and HER2-IHC 0 (n=590), as reference. Hormone Receptor (HR) expression has also been considered for subgroup analysis. Results: At first ABC diagnosis, < 1% pts had unresectable locally advanced BC (ULABC) in both groups, 31% de novo metastatic BC in HER2-low and 20% in HER2-IHC0 groups. Less than 1% were male, 99% Caucasian and ~71% postmenopausal. Median age was 60 years, being similar between both groups (range 26-96). Family history of BC and/or ovarian cancer was reported in 32% pts in HER2-low and 29% in HER2-IHC0. Germline BRCA1/2 mutation was higher in HER2-low (14/40=35%) in reference to HER2-IHC0 (14/64=22%) (p=0.14). Relevant information summarized by HR expression and HER2 status are detailed in the table below. Visceral disease was similar in both total groups (58% in HER2-low vs HER2-IHC0 56%), but slightly higher in HR- HER2-low pts (81% vs 64%), and 84% and 90% pts had ≤3 metastatic locations, in HER2-low and HER2-IHC0 groups, respectively. Distribution of 1st-line therapies was also similar between both groups, being endocrine therapy (ET) plus biological therapy (BT) (HER2-low 39% vs HER2-IHC0 36%) and ET (HER2-low 30% vs HER2-IHC0 28%), while chemotherapy (CT) (HER2-low 13% vs HER2-IHC0 16%) and CT plus BT (HER2-low 9% vs HER2-IHC0 10%) were more frequent in HR- pts. A 2nd-line therapy was reported in 70% HER2-low pts and 67% HER2-IHC0 pts. The median time to progression (TTP) at 1st-line therapy in HER2-low pts was 11 mo (0-66), being similar in HER2-IHC0 pts, but the higher difference was observed in relation to HR expression. Treatments in 2nd-line were similar in both groups, CT and ET/BT were the most frequent totally. Median duration of 2nd-line therapy was ~6 mo; progressive disease (PD) was reported in 85% pts. A 3rd-line therapy was reported in 74% HER2-low and 69% HER2-IHC0 pts. The median time to progression (TTP) to 3rd-line therapy was 6 mo, being similar in HER2-low and HER2-IHC0 pts .The most frequent 3rd-line therapies were CT and ET/BT in both groups, as in 2nd-line. Median duration of 3rd-line therapy was 4 mo; PD was reported in 84% HER2-low pts and 82% HER2-IHC0 pts. Median (95% confidence interval [CI]) PFS at 1st, 2nd and 3rd lines (mo) were 14 (12-16), 6 (5-7) and 6 (5-6) in HER2-low pts, being similar in HER2-IHC0. OS was comparable in all soubgroups analyzed, however, differences were observed regarding HR status. Conclusions: Our results show that HER2-low BC pts have similar characteristics than HER2-IHC0 BC pts. There are differences in therapy outcomes in terms of survival and prognosis, particularly in HR- tumors, being better in HER2-low BC pts. It could be related to the fact that these tumors have a specific biology, but more evidence is needed. Citation Format: Isabel Álvarez, Angel Guerrero-Zotano, Ariadna Tibau, Catalina Falo, María Hernández, Ana Miguel, Raquel Andrés, Álvaro Rodríguez-Lescure, Miguel Corbellas, Sara López-Tarruella, Purificación Martínez, Cesar A Rodríguez, Diego Malón, María Marin, Mª José Echarri, Antonio Antón, Josefina Cruz, Diana Moreno, J. Ignacio Chacón, Ruth Campo, Andrea Blasco, Susana Bezares, Federico Rojo, Silvia Antolin. Features and survival outcomes of HER2-low patients from a prospective registry of unresectable locally advanced or metastatic breast cancer: GEICAM/2014-03 (RegistEM) [abstract]. In: Proceedings of the 2022 San Antonio Breast Cancer Symposium; 2022 Dec 6-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2023;83(5 Suppl):Abstract nr P4-07-30.

Background: Over the last years, the treatment of HER2-positive (HER2+) breast cancer (BC) patients (pts) has been changing because of the development of new anti-HER2 agents. In the current analysis, we describe the features, treatment patterns, progression-free survivall (PFS) and overall survival (OS) outcomes of BC pts with HER2 + (immunohistochemistry [IHC] 3+ or IHC 2+ and in situ hybridization [ISH]+), following ASCO/CAP 2018 guidelines in the most recent tumor lesion before the 2nd-line. Methods: The RegistEM study is an ongoing BC registry study that is providing prospective data from around 1900 pts diagnosed with advanced BC (ABC) between 01/Jan/2016 and 31/Dec/2019, in 38 Spanish institutions from GEICAM network. In this analysis, 296 HER2+ BC pts have been included, representing the 18% of pts available in the database at the cut-off date (08/Apr/2022), with ABC diagnosis before 2019 (n=1559). Results: At first ABC diagnosis, 58% (n=173) pts had recurrent disease (>36 months [mo] from initial BC diagnosis in 62%), 41% (n=120) de novo metastatic BC and 1% (n=3) unresectable locally ABC (ULABC); the median age was 58 years, 68% were postmenopausal and there was only 1 male pt. From total 296 pts, 66% had hormone receptor expression [HR+]; the BC subtype was assessed in tumor tissue from the breast (58%) or a metastatic lesion (34%), and in 8% pts, HER2 positivity was observed after the 1st-line. Family history of BC and/or ovarian cancer was reported in 28% pts, and a hereditary-risk genetic test was performed in 26% pts (n=74/282). Germline BRCA1/2 and TP53 genetic testing were reported in 14 and 26 pts respectively, being mutated in 3/14 (21%) and 5/26 (19%) pts. Bone (50%), lymph nodes (49%), liver (35%), lung (31%), soft tissue (8%) and central nervous system (CNS), mostly in brain (8%), were the main metastatic sites. One hundred pts were diagnosed with CNS metastases: 24 at baseline, 48 during the 1st-line and 28 in subsequent lines. Additional data according to HR status and type of ABC are detailed in the table below, showing a worse prognosis in absence of HR expression. In HR- pts, bone metastases were less frequent and lymph nodes metastases more frequent compared to HR+ pts. Visceral disease was present in 69% (66% in HR+ and 74% in HR-; non-statistically significant) pts and ≈80% had ≤3 (54%, ≤2) locations involved. The most common therapies by line were: 1) 1st-line: Chemotherapy (CT) + biological therapy (BT) (38%), CT + BT+ endocrine therapy (ET) (35%), and ET + BT [11%]; 2) 2nd-line: BT (55%), CT + BT (20%) and ET + BT (15%); 3) 3rd-line: CT + BT (49%) and BT (31%). The median (95% confidence interval [CI]) progression-free survival (PFS) on 1st, 2nd and 3rd line was 18 (15-22), 8 (7-9) and 6 (5-8) mo, respectively. The median (95% CI) overall survival (OS) from ABC diagnosis was 43 (40-49) mo. These survival outcomes were higher in HR+ pts, however, the differences were only statistically significant in OS (p=0.006; log-rank). At database cut-off date, death was reported in 47% pts. Conclusions: In spite of the anti-HER2 therapies administered in the advanced setting, the HR expression is a relevant prognostic factor, with a clinically and statistically significant impact in OS, improving the outcomes of HR+ pts. Citation Format: Sara López-Tarruella, Angel Guerrero-Zotano, Josefina Cruz, Silvia Antolin Novoa, Purificación Martínez, María Hernández, César A Rodríguez, J. Ignacio Chacón, Ariadna Tibau, Catalina Falo, Álvaro Rodríguez-Lescure, Mireia Margelí, Sonia Servitja, Raquel Andrés, María Galán-Gramaje, Encarna Adrover, Ana Miguel, Rafael Villanueva, Silvia Varela, Ruth Campo, Mª José Escudero, Susana Bezares, Federico Rojo, Isabel Álvarez. Real-world data of Advanced Breast Cancer (ABC) patients with HER2-positivity before the second-line therapy: data from the observational study GEICAM/2014-03 (RegistEM) [abstract]. In: Proceedings of the 2022 San Antonio Breast Cancer Symposium; 2022 Dec 6-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2023;83(5 Suppl):Abstract nr P4-07-38.

The prevalence of hepatitis C virus (HCV) infection is higher in people with psychiatric disorders compared to the general population. In addition, patients with severe mental illness are frequently affected by substance abuse, which increases the risk of blood-borne viral infections. Epidemiological studies in samples of hospitalised individuals with chronic mental disorders and dual diagnosis (DD) are lacking. The objective of this study was to investigate the prevalence of HCV infection in a sample of in-patients with severe mental illness.This was a retrospective observational study. All patients meeting selection criteria admitted to the Medium-Term Psychiatric Unit of the University of Salamanca Health Care Complex between 2007 and 2018 were included. The primary endpoint was the prevalence of HCV infection. The secondary endpoint comprised the characteristics influencing the occurrence of HCV infection in these patients.A total of 497 admissions were included and patients' last admission data were considered for analyses (n=345). The overall prevalence of HCV infection was 3.8% and reached 14.3% among DD patients, who showed a higher prevalence than those without this condition (14.3% versus 3.1%, p=0.009). HCV RNA was detected in 6 individuals at diagnosis who received DAA treatment reaching sustained virological response.The prevalence of HCV infection in our sample was higher than in the general population, especially among DD patients. Despite the multiple barriers to access healthcare by patients with chronic mental illness, efforts to include this population in screening and treatment are mandatory.

11 Páginas.-- 5 Figuras.-- 2 Tablas, We have previously reported the in vitro hypocholesterolemic, anti-inflammatory, and antioxidant effects of Alcalase-generated lupin protein hydrolysate (LPH). Given that lipoprotein deposition, oxidative stress, and inflammation are the main components of atherogenesis, we characterized the LPH composition, in silico identified LPH-peptides with activities related to atherosclerosis, and evaluated the in vivo LPH effects on atherosclerosis risk factors in a mouse model of atherosclerosis. After 15 min of Alcalase hydrolysis, peptides smaller than 8 kDa were obtained, and 259 peptides out of 278 peptides found showed biological activities related to atherosclerosis risk factors. Furthermore, LPH administration for 12 weeks reduced the plasma lipids, as well as the cardiovascular and atherogenic risk indexes. LPH also increased the total antioxidant capacity, decreased endothelial permeability, inflammatory response, and atherogenic markers. Therefore, this study describes for the first time that LPH prevents the early stages of atherosclerosis., Ministerio de Economía y Competitividad, Gobierno de España [AGL2012-40247-C02-01 and AGL2012-40247-C02-02], Consejería de Salud, Junta de Andalucía [PC-0111-2016-0111 and PEMP-0085-2020], and the Programa PAIDI from the Junta de Andalucía [CTS160]. G.S.-S. was supported by Formación Profesorado Universitario grants from the Ministerio de Educación, Cultura y Deporte, Gobierno de España [FPU16/02339]. I.C.-C. was supported by a postdoctoral fellowship from the Consejería de Economía, Conocimiento, Empresas y Universidad, Junta de Andalucía [DOC_00587/2020]. N.Á.-S. was supported by a fellowship from the Red Temática de Investigación Cooperativa en Envejecimiento y Fragilidad (RETICEF) [RD12/0043/0012 from the Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación, Gobierno de España]. B.R.-O. was supported by a grant from the Programa de Empleo Juvenil of Ministerio de Empleo y Seguridad Social, Gobierno de España [EJ-086]. A.I.Á.-L. was funded by the Consejería de Salud, Junta de Andalucía [PI-0136-2019]. I.B. was supported by the VI Plan Propio de Investigación y Transferencia of Universidad de Sevilla [VI PPIT-US].

La ausencia de una actualización de información gastronómica tanto de los platos ofertados y las técnicas culinarias para la elaboración de cada uno, basado en el conocimiento de antaño. El estudio realizado estuvo enfocado en rescatar los conocimientos de la cocina ancestral basado en la herencia de información trasladada de generación en generación en el Barrio Las Herrerías de la ciudad de Cuenca mediante un registro de saberes gastronómicos. La metodología planteada en esta propuesta fue cualitativa con un diseño etnográfico y recopilación documental, además se desarrolló trabajo de campo mediante la observación, también se realizó el levantamiento de recetas del sector en mención a través de entrevistas estructuradas, adicionalmente se desarrolló una ubicación de los establecimientos más representativos de gastronomía tradicional en base a una georreferenciación, el registro de información se desarrolló mediante fichas sistematizadas que fueron obtenidas en referencia al modelo del Instituto Nacional de Patrimonio Cultural. Como resultados se desarrolló un recetario con las técnicas y conocimientos de los diferentes platos tradicionales en relación a los envueltos elaborados en el Barrio Las Herrerías, pese a estar en un mismo sector se determina una caracterización en cuanto a sabor, aroma, texturas y técnicas de cocción, dejando a relucir un potencial cultural y de tradiciones del sector de estudio, que podría enfocarse en nuevas promesas de turismo gastronómico.

Introducción: La crisis generada por el virus SARS-CoV-2 ha supuesto un gran impacto en el plano sanitario, económico y social. En el ámbito sanitario, las circunstancias excepcionales en las que se han producido muchos fallecimientos han obligado a los profesionales a realizar la comunicación de malas noticias de manera remota a través del teléfono. Objetivo: El presente artículo pretende sintetizar de forma organizada las principales pautas que contribuyen a una comunicación eficaz, permitiendo a los profesionales reducir sus niveles de estrés y lograr una mayor eficiencia a la hora de comunicar un fallecimiento telefónicamente en una situación de emergencia como la actual pandemia. Metodología: Se realizó una revisión con base en la evidencia científica recogida en la bibliografía existente sobre el tema. Se consultaron las bases de datos PubMed, Scopus, y el buscador Google Académico. Resultados: Para comunicar malas noticias, el profesional debe contar tanto con unas buenas habilidades de comunicación, como con un manejo adecuado de unas pautas técnicas básicas de actuación. Conclusión: El uso de pautas con cierto grado de estructura, puede aumentar la eficacia de las comunicaciones de malas noticias a través del teléfono en situaciones de emergencia, maximizando los beneficios tanto para el receptor como para el emisor.

Esti artículu analiza la recuperación de la tradición y les nueves formes d’activismu y texíu de redes comunitaries na música n’Asturies tres de la crisis financiera de 2008. Col estudiu específicu d’artistes como Rodrigo Cuevas, ún de los nuevos músicos con mayor reconocencia dientro y fuera d’Asturies; Ún de Grao, el proyeutu musical de Xosé Martínez Álvarez; miembru de la conocida como xeneración «post-folk», que busca dar puxu otra vuelta a la tonada; y el Coru Al Altu la Lleva, que, nacíu de l’atmósfera de l’asociación Caja de músicos, sigue cola tradición de la música coral poniendo un procuru especial nel activismu, mesmo xunto al perconocíu músicu asturianu Nacho Vegas que de manera independiente. La música asturiana de recién amuesa tendencies nueves na so rellación col tiempu y l’espaciu. Músicos y músiques busquen participar de manera global, ensin dilise en patrones definíos, al traviés de les sos diferencies y activismu. Dalgunos de los problemes colos que los músicos y músiques s’identifiquen son el papel que xueguen les llingües y cultures que conviven nun territoriu, el revivir un pasáu cuestionando ciertes idees de xeneraciones anteriores, la reconocencia de la diversidá sexual, la tendencia neorrural depués de la depresión económica y la pandemia y la medra del activismu comunitariu depués del movimientu de los Indignados resultáu de la crisis financiera de 2008. Pel camín de la música, Asturies y la llingua asturiana participen y respuenden a les tendencies locales, nacionales ya internacionales que, sofitaes na tradición, creen maneres úniques de resignificación del pasáu y d’influyencia del presente y del futuru.

Background: Autosomal dominant polycystic kidney disease (ADPKD) is the most common hereditary cause of end-stage kidney disease. Currently, tolvaptan is the only treatment that has proven to delay disease progression. The most notable side effect of this therapy is drug-induced liver injury; however, recently, there have been two reports of creatine kinase (CK) elevation in ADPKD patients on tolvaptan treatment. We set out to monitor and determine the actual incidence of CK elevation and evaluate its potential association with other clinical factors. Methods: This is an observational retrospective multicenter study performed in rapidly progressive ADPKD patients on tolvaptan treatment from Barcelona, Spain. Laboratory tests, demographics, treatment dose, and reported symptoms were collected from October 2018 to March 2021. Results: Ninety-five patients initiated tolvaptan treatment during follow-up. The medication had to be discontinued in 31 (32.6%) patients, primarily due to aquaretic effects (12.6%), elevated liver enzymes (8.4%), and symptomatic or persistently elevated CK levels (3.2%). Moreover, a total of 27 (28.4%) patients had elevated CK levels, with most of them being either transient (12.6%), mild and asymptomatic (4.2%), or resolved after dose reduction (3.2%) or temporary discontinuation (2.1%). Conclusion: We pre­sent the largest cohort that has monitored CK levels in a real-life setting, finding them elevated in 28.4% of patients. More research and monitoring will help us understand the clinical implications and the pathophysiological mechanism of CK elevation in this population.

Objetivo: Evaluar el nivel de satisfacción laboral de los profesionales de enfermería de un hospital de seguridad social. Material y métodos: Estudio de cohorte transversal, descriptivo; se utilizó un muestreo no probabilístico por conveniencia, constituido por 49 profesionales de enfermería. Para evaluar la satisfacción se empleó el cuestionario Font-Roja, el cual se valora a través de una escala tipo Likert. Resultados: Se obtuvo un nivel de satisfacción media (89,8 %); la mayor parte de la población es de sexo femenino; el rango de edad de los profesionales con insatisfacción laboral está entre los 30 y 34 años. Respecto al estado civil, los casados (6,1 %) refieren insatisfacción; se observó que, a mayor antigüedad en la institución, mayor es el grado de insatisfacción; asimismo, los profesionales con turno y servicio mixto se encuentran insatisfechos; las dimensiones peor valoradas son III (competencia profesional), V (promoción profesional), VI (relación interpersonal con sus jefes), VII (relación interpersonal con los compañeros) y VIII (características extrínsecas del estatus). Conclusión: La satisfacción laboral de los profesionales de enfermería de un hospital de segundo nivel alcanza un rango medio.

Pneumococcal conjugate vaccines (PCVs) have decreased pneumonia in children. Colombia introduced mass vaccination with PCV10 in 2012.Cases of pneumococcal pneumonia from 10 hospitals were included. Two periods were compared: pre-PCV10: 2008-2011 and post-PCV10: 2014-2019. The objective was to compare epidemiological and clinical characteristics before and after PCV10 vaccination.A total of 370 cases were included. Serotypes 1 (15, 11.2%) and 14 (33, 24.6%) were the most frequent in the pre-PCV10 period, with only 4 (3%) cases of serotype 19A and 1 case (0.7%) serotype 3. From the pre-PCV10 period to the post-PCV10 period, cases of serotypes 1 (6, 3.1%) and 14 (1, 7.8%) decreased, while cases of serotypes 19A (58, 30.2%), serotype 3 (32, 16.7%) and 6A (7, 3.6%) increased (p 0.001); complicated pneumonia (CP) increased significantly (13.4% to 31.8%) (p 0.001); hospitalizations increased from 8 (5.5-15) to 12 (7-22) days (p 0.001); and the frequency of PICU admission increased from 32.8% to 51.6% (p = 0.001). The use of ampicillin-sulbactam (0.7% to 24%) and ceftriaxone/clindamycin (0.7% to 5.7%) increased in the post-PCV10 period. The duration of empirical antibiotic treatment was 7 (4-11) days in the pre-PCV10 period and increased to 10 (6-17) days (p 0.001) in the post-PCV10 period. Lethality showed a slight nonsignificant increase (7.5% vs. 9.9%; p = 0.57) in the post-PCV10 period.PCV10 significantly decreased cases of serotypes 1 and 14, with an increase in cases of serotypes 19A, 3 and 6A, which were the predominant serotypes and had greater severity (e.g., admission to the PICU, CP and more resistance, with an increase in the use of broad-spectrum antibiotics and longer hospitalization) and subsequently included in PCV13. Current data support national and regional evidence on the importance of replacing PCV10 with a higher valence that includes 19A, such as PCV13, with the aim of reducing circulation, particularly of this serotype.

La alimentación ha sido siempre parte fundamental de toda sociedad, es así que cada pueblo ha ido desarrollando diferentes sistemas de alimentación adaptados a su realidad. Es por eso que, el estudio propone revalorizar la cocina tradicional a partir de ollas de barro, realizando pruebas de análisis sensorial empleando las cualidades de este utensilio para la elaboración de caldos tradicionales en el Austro. A través de la observación de campo, se recolectó información sobre la cocina tradicional ancestral nombrando las técnicas de cocción en ollas de barro, su origen, fabricación, así como su función; con la finalidad de dar a conocer la historia de este utensilio, que, con el transcurso de los años ha ido perdiendo valor debido a las nuevas tecnologías y métodos de cocción. Se hizo especial énfasis en la cocina del Austro, reconociendo a las ollas de barro como utensilio ancestral en la elaboración de caldos tradicionales de gallina criolla, patas y mocho, además de destacar los ingredientes y recetas de nuestra cocina. Finalmente, a través de pruebas triangulares de análisis sensorial, realizadas a panelistas se determinó las características organolépticas de los caldos en las diferentes muestras, comprobando así la diferencia de sabor que aportan las ollas de barro frente a otros materiales, potencializando significativamente el uso de este material en nuestras cocinas.

Background: The RegistEM study is a non-interventional study that is providing prospective data from around 1900 ABC pts (females and males) diagnosed with advanced disease between 01/Jan/2016 and 31/Dec/2019, either after recurrence or at 1st diagnosis, in 38 Spanish sites representative of the national territory and whose investigators are GEICAM members. Methods: In the current analysis (cut-off date 10/May/2021, ongoing database), we describe the features of 279 pts included in the RegistEM study, with HER2+ (immunohistochemistry [IHQ] 3+, IHQ 2+ and in situ hybridization [ISH]+) tumors at any time of their ABC (5% after the 1st-line therapy). This subgroup has been evaluated because of the interest from a clinical perspective. Multivariate Cox analysis aiming to identify factors associated with overall survival (OS) were built. Results: 279 pts were identified, representing the 15% pts available in the database at the cut-off date. At first ABC diagnosis, 48% pts had recurrent BC (>12 months [mo] from initial BC diagnosis in 93%), 51% de novo metastatic BC and 1% unresectable locally advanced BC (ULABC). The median age was 59 years, 98% were white , 71% postmenopausal and only 1 male was part of this subset. Considering the BC subtype assessed in the most recent tumor lesion before the 1st-line therapy, 264 pts wereHER2 positive (67% with hormone receptor [HR]+). Family history of BC and/or ovarian cancer was reported in 31% pts, and an hereditary-risk genetic test was performed in 25% (66/267 pts). BRCA1/2 and TP53 mutations were reported in 4/20 and 4/19 pts, respectively, and p53 overexpression in 20/46 pts. Lymph nodes (56%), bone (49%), liver (34%), lung (33%), soft tissue (10%) and brain (8%) were the main metastatic sites. Additional data according to HR status and type of ABC are detailed in the table below. In HR- pts, bone metastases were less frequent and lymph nodes metastases more frequent compared to HR+ pts. Visceral disease was present in 68% pts and ≈75% had ≤3 (47% ≤2) locations involved. The most common therapies by line were: 1) 1st-line: CT + dual anti-HER2 blockade (3%), chemotherapy (CT) (almost in all pts taxane-based)+dual anti-HER2 blockade + endocrine therapy (ET) (mainly aromatase inhibitors) (35%), and ET + anti-HER2 blockade or ET + cyclin-dependent kinases 4/6 inhibitors (11%); 2) 2nd-line: anti-HER2 blockade (56%) [mostly an antibody-drug conjugate (90%)], CT + anti-HER2 blockade (18%) and ET + anti-HER2 blockade (14%); 3) 3rd-line: CT + anti-HER2 blockade (55%) and anti-HER2 blockade (22%). The median time-to-progressions to 1st-, 2nd- and 3rd-line were 14, 5, and 4 mo, respectively. A 4th-line therapy was reported in 52% of pts who received a 3rd-line. At database cut-off date, death was reported in 34% of pts. The median OS of this subset of pts was 41 mo (36-49). In a multivariate Cox regression analysis, the following variables were significantly related with worse survival (from ABC diagnosis): Brain (HR=2.62; 95% CI, 1.02-6.73) and Visceral no Brain involvement (HR=2.15; 95% CI, 1.02-4.53) compare to only soft tissue lesions; early stage at first diagnosis (HR=1.77; 95% CI, 1.15-2.73); HR- (HR=1.70; 95% CI, 1.11-2.60) and age (HR=1.04; 95% CI, 1.02-1.07). Conclusions: In this cohort of HER2+ pts with advanced disease, half of them had de novo ABC which was associated with better OS. The median PFS in 1st- and 3rd-line were slightly better in HR+ pts, and in 2nd-line was similar between HR+ and HR- cohorts. HR+181 (67%)HR- 91 (33%)Recurrent EBC134 (48%)ULABC or de novo M1 145 (52%)Time to recurrence >12 mo in EBC pts., n8435125NALocation of metastaticsites, nBoneBrainLiverLungLymph nodesSoft tissue104 10 62 57 90 1829 9 3232 64 1059 15 36 47 51 2278 6 59 46 1056Líne123123123123n180935690492613385521446333Deaths, n211112111161715141584Therapies by line, nET/BT261922101910212100ET12441011022323CT/BT/ET935031038305830CT/BT4017307581552182366924CT3572353711211BT6431373651145143385The most frequent therapies, nCT + dual anti-HER2 blockade + ET8623331551CT + single-agent HER2 blockade + ET522CT + dual anti-HER2 blockade3493644391016252CT + single-agent HER2 blockade4825641588211521CT5573354711411ET*22105212162644ET + HER2 blockade1213411662782Anti-HER2 blockade6431073651145143382Median duration of treatment, mo125585310441064TTP (mo), median (range)15(1-47)5(1-32)5(0-18)11(2-38)5(1-27)4(2-12)12 (1-47)5(1-26)4(0-17)17(2-45)7(1-32)4(1-18)Median PFS, mo14561154------HR: hormone receptor; EBC: early breast cancer; ULABC: unresectable locally advanced breast cancer; M1: metastatic; mo: month; ET: endrocrine therapy; BT: biological therapy; CT: chemotherapy; TTP: time-to-progression; PFS: progression-free survival. *ET includes aromatase inhibitors or selective estrogen receptor degraders, as single-agents or combined with cyclin-dependent kinases 4/6 inhibitors. Citation Format: Isabel Álvarez, Ángel Guerrero-Zotano, Josefina Cruz, Purificación Martínez, María Hernández, César A Rodríguez, Álvaro Rodríguez-Lescure, Silvia Antolín, Encarna Adrover, Raquel Andrés, Catalina Falo, Jose Ignacio Chacón, Ana Miguel, Sonia Servitja, Maria Galán Gramaje, Mireia Margelí Vila, César Gómez Raposo, María Jose Echarri, Rafael Villanueva, Ariadna TIbau Martorell, Silvia Varela Ferreiro, Ruth Campo, Juan Jose Miralles, Susana Bezares, Federico Rojo, Sara López-Tarruella. Features of HER2+ metastasic patients (pts) from a prospective registry of advanced breast cancer (ABC), GEICAM/2014-03 (RegistEM) [abstract]. In: Proceedings of the 2021 San Antonio Breast Cancer Symposium; 2021 Dec 7-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2022;82(4 Suppl):Abstract nr P1-15-04.

Neoadjuvant endocrine therapy in oestrogen receptor (ER) positive HER2 negative breast cancer allows real-time evaluation of drug efficacy as well as investigation of the biological and molecular changes that occur after estrogenic deprivation. Clinical and pathological evaluation after neoadjuvant endocrine therapy may be used to obtain prognostic and predictive information of tumour response to decide adjuvant treatment. In this setting, clinical scales developed to evaluate response after neoadjuvant chemotherapy are not useful and there are not many validated biomarkers to assess response to neoadjuvant endocrine therapy beyond Ki67 expression and preoperative prognostic index (PEPI) score. In this prospective study, we extensively analysed radiological (by ultrasound and magnetic resonance imaging) and pathological tumour response of 104 postmenopausal patients with ER+/HER2-resectable breast cancer, treated with neoadjuvant endocrine treatment for a mean of 7 months prior to surgery. Our results show that radiological evaluation by both USS and MRI underestimate pathological tumour size, although they support the use of MRI over USS to clinically assess tumour response. In addition, we propose that the tumour cellularity size, calculated as the product of the percentage of residual tumour cellularity in the surgical specimen and the tumour pathological size, could become a new tool to standardize response assessment to NET given its good correlation with and potential added value to existing biomarkers. Our findings shed light on the dynamics of tumour response to neoadjuvant endocrine therapy, challenge the paradigm of the ability of NET to decrease surgical volume and point to the utility of the tumour cellularity size to quantify the scattered tumour response usually produced by endocrine therapy.

Objectives: Implement a mobile application to control COVID-19 in all its variants, containing or reducing the spread of the virus during the return to face-to-face classes at the ITCG. Methodology: The project was developed in Android Studio using different libraries and technological resources. You have access to a web server with a MySQL database. The application allows scanning the QR code of the visited site from the mobile application, reporting a positive case and vaccine reactions, monitoring any unusual adverse effects, as well as receiving notifications via SMS. Contribution: Contain the advance of the virus, avoiding saturating health systems, monitoring users in the event of any eventuality. The application was distributed to the student community of the Technological Institute of Ciudad Guzmán (ITCG) during the application of the first dose of the AstraZeneca vaccine, to monitor any symptoms, as well as positive cases during the return to face-to-face classes.

Se realiza un comentario a la STC 148/2021, de 14 de julio, en relación con la declaración del estado de alarma en marzo de 2020, a la STC 183/2021 que declara inconstitucional la prórroga de los seis meses del estado de alarma, así como el nombramiento de autoridades competentes delegadas, y enlazando estos comentarios con el contenido de la STC 168/2021 en relación con el recurso de amparo presentado contra el Acuerdo de la Mesa del Congreso de 19 de marzo de 2020. La primera sentencia, adoptada por la exigua mayoría de seis votos a favor y cinco en contra (posiciones contrarias que se han traducido en los correspondientes votos particulares), declara inconstitucional parte del Real Decreto 463/2020 y los términos «modificar o ampliar» del apartado 6 del artículo 10, en la redacción resultante del artículo único, apartado 2, del Real Decreto 465/2020. La segunda STC estima parcialmente el recurso de inconstitucionalidad contra varios preceptos del Real Decreto 926/2020, de 25 de octubre, por el que se declaró el segundo estado de alarma; contra varios apartados de la Resolución de 29 de octubre de 2020, del Congreso de los Diputados y contra el art. 2, la disposición transitoria única y disposición final primera del Real Decreto 956/2020, de 3 de noviembre, por el que se prorrogó el estado de alarma por el Real Decreto 926/2020. La sentencia declara la inconstitucionalidad de la prórroga de los seis meses y el nombramiento de autoridades competentes delegadas fijada en la norma impugnada. Y la tercera STC señala que la declaración del estado de alarma, como la de cualquiera de los otros dos estados de excepción y de sitio, no puede en ningún caso interrumpir el funcionamiento de ninguno de los poderes constitucionales del Estado y, de modo particular, el Congreso de los Diputados.

La musicoterapia ha demostrado ser beneficiosa para muchos sectores poblacionales. Sin embargo, el número de investigaciones relacionadas con la ceguera o déficit visual era mucho menor al compararlas con el de aquellas realizadas en otros campos. Así pues, el presente trabajo consiste en la muestra de los resultados obtenidos de la puesta en marcha de un programa de innovación psicomotriz y emocional a través de la musicoterapia. Éste estuvo dirigido a personas usuarios y usuarias de la ONCE de Valladolid con deficiencia visual. El estudio ha consistido en un seguimiento grupal a través de la evaluación de inicio, de proceso y final, que muestran la evolución general de los asistentes por medio de una recogida de datos a través del Sistema de Evaluación Musicoterapéutica para Personas con Alzheimer y otras Demencias (SEMPA).

23 Páginas.-- 4 Figuras.-- 4 Tablas, Background: Over the last two decades, the demand for meat-free food has increased for health, environmental, and animal welfare reasons. Thus, research on the value of vegetable compounds as beneficial agents for human health has gained much attention. In particular, great interest has been shown in protein hydrolysation since this food technology facilitates the release of encrypted peptides with biological activities. In this sense, during the last 10 years, the biological activities of Lupinus spp. protein hydrolysates (LPHs) have been extensively studied. Scope and approach: The aim of this review is to address all studies (in silico, in vitro, and in vivo) in which the biological activities (antioxidant, anti-inflammatory, etc.) of LPHs were described, both the whole hydrolysate and the derived peptides. Moreover, the physicochemical characteristics of LPH peptides are evaluated, and challenges and future perspectives for their fast application have been discussed. Key findings and conclusions: LPHs exert many important biological effects in both the in vitro and in vivo systems. The main activities of LPHs described are antioxidant, immunomodulatory, hypotensive, hypoglycemic, and hypolipidemic. These findings point to LPHs as a possible new nutraceutical for human health, capable of preventing or treating some chronic diseases such as diabetes, dyslipidemia, and atherosclerosis. Finally, the development of a new generation of nanonutraceuticals to improve the metabolic stability and bioactivity of LPHs has been discussed., This work was funded by the Spanish Government, Ministerio de Economía y Competitividad (AGL2012-40247-C02-01 and AGL2012-40247-C02-02), the Andalusian Government Ministry of Health PC-0111-2016-0111, PEMP 0085–2020 (co-financed with FEDER funds, call Resolution of 7 July 2021 of the General Secretary for Research, Development and Innovation in Health, which calls for grants to finance research, development and innovation in biomedicine and health sciences in Andalusia by 2021), and the PAIDI Program from the Andalusian Government (CTS160). ICC was supported by a postdoctoral fellowship from Consejería de Transformación Económica, Industria, Conocimiento y Universidades (Junta de Andalucía) (DOC_00587/2020). GSS was supported by a FPU grant from Ministerio de Educación, Cultura y Deporte (Gobierno de España) (FPU16/02339). AIAL was funded by Consejería de Salud (Junta de Andalucía) (PI-0136-2019 and PEMP 0085–2020).

Fermentation of orange juice (OJ) by Pichia kluyveri enhances the content of melatonin, a molecule with potent antioxidant effect. This study explores the levels of urine 6- sulfatoxymelatonin (6-SMT) in healthy subjects after fermented orange juice (FOJ) intake, and their association with antioxidant activity status. Nine participants ingested 500 mL of FOJ and their urine was collected at baseline and after 2, 5, 10, 15 and 24 h. After a two-week washout period, the intervention was repeated with OJ. 6-SMT levels were quantified by ELISA and antioxidant activity by TAC, FRAP and ORAC assays. A significant increase in both 6-SMT levels and antioxidant activity in urine was observed after FOJ ingestion compared to OJ. A positive correlation between TAC and 6-SMT levels was observed only after FOJ intake. This study shows for the first time that fermentation process increases melatonin bioavailability of OJ associated with an enhancement in antioxidant status., This research was funded by the Andalusian Government [project P09-AGR4814M] and the PAIDI Program from the Andalusian Government [CTS160, BIO311]. I.C.-C. was supported by a postdoctoral fellowship from the Andalusian Government Ministry of Economy, Knowledge, Business, and University [DOC_00587/2020]. G.S.-S. was supported by a FPU grant from the Spanish Ministerio de Educación, Cultura y Deporte [FPU16/02339]. N.A.-S. was supported by a fellowship from the National Net RETICEF for Aging Studies [RD12/0043/0012 from the Instituto de Salud Carlos III, Spanish Ministerio de Ciencia e Innovación].

[Background] Iron metabolism plays an essential role in cellular functions. Since virologically suppressed chronic HIV-infected subjects under effective antiretroviral treatment (ART) exhibit a persistent immune dysfunction that leads to comorbidities, iron homeostasis may be relevant in this context. We aimed to explore iron metabolism in virologically suppressed chronic HIV infected subjects under a successful ART., [Methods] In this retrospective study, traditional iron metabolism biomarkers (total iron, ferritin, transferrin, and transferrin saturation index), as well as soluble transferrin receptor (sTfR), hepcidin, and inflammatory markers were determined in virologically suppressed chronic HIV-infected subjects under at least 2 years of ART (HIV) who also had >350 CD4-T-cells/mm3 (N=92) from Spain. As controls, we collected non-HIV age-matched healthy donors (Young, N=25) and elderly subjects (>65 years old; Elderly; N=25). Additionally, an external group of non-HIV patients with ferritin, [Results] We selected samples from HIV-infected subjects (aged 42[34-47], 95% males), young age-matched (aged 40[30-58], 60% males), and elderly controls (aged 82[78-88], 100% males). Compared to both healthy (Young and Elderly) groups, HIV exhibited decreased iron, transferrin saturation, and sTfR, and increased ferritin, but similar hepcidin levels. Notably, associations between sTfR and iron (Young, r=-0.587, p=0.002; Elderly, r=-0.496, p=0.012) or transferrin saturation index (Young, r=-0.581, p=0.002; Elderly, r=-0.489, p=0.013) were negative in both controls while positive in HIV (r=0.464, p, [Conclusions] Virologically suppressed chronic HIV-infected patients under successful ART exhibit altered levels of iron metabolism modulators suggesting a complex functional iron deficiency., This work was supported by grants from the Fondo de Investigación Sanitaria [FIS; PI18/01216 and PI21/00357] which is co-funded by Fondos Europeos para el Desarrollo Regional (FEDER) “Una manera de hacer Europa”, the Instituto de Salud Carlos III [FI19/00298 to VG-R, CM19/00051 to IO-M and CD19/00143 to AB-R], the Consejería de Transformación Económica, Industria, Conocimiento y Universidades [DOC_01646 to MP-B] and the Consejería de Salud y Familias of Junta de Andalucía through the “Nicolás Monardes” [C-0013-2017 to YMP]. The HIV BioBank, integrated in the Spanish AIDS Research Network, is supported by Instituto de Salud Carlos III, Spanish Health Ministry (Grant n° RD06/0006/0035, RD12/0017/0037 and RD16/0025/0019) as part of the Plan Nacional I+D+i and cofinanced by ISCIII- Subdirección General de Evaluación y el Fondo Europeo de Desarrollo Regional (FEDER)”. The RIS Cohort (CoRIS) was funded by the Instituto de Salud Carlos III through the Red Temática de Investigación Cooperativa en SIDA (RIS C03/173, RD12/0017/0018 and RD16/0002/0006) as part of the Plan Nacional I+D+i and cofinanced by ISCIII-Subdirección General de Evaluación and the Fondo Europeo de Desarrollo Regional (FEDER).

El presente libro recoge en gran parte reflexiones, apuestas metodológicas y la intención de entretejer la academia con las realidades sociales. El grupo de investigación Cuchavira, poder, socialización y conocimiento nace en el año 2011, atendiendo algunos cuestionamientos que transitaban alrededor de tres elementos estructurales. El primero avanzaba hacia la comprensión de la función social de la educación, el segundo orientaba la mirada hacia las implicaciones políticas de la propuesta educativa social-comunitaria de la UNAD y el tercer elemento agenciaba la responsabilidad del tejido entre la académica y lo comunitario, en el esfuerzo de contribuir hacia el entendimiento de un conflicto social armado en Colombia. Desde estas consideraciones, el grupo Cuchavira ha generado diferentes espacios de trabajo, que van desde la creación de semilleros de investigación en las ciudades de Bogotá, Villavicencio, Medellín, Popayán y Valledupar, pasando por la participación y ejecución de proyectos de investigación con los semilleros e investigadores profesores, hasta la integración en espacios de construcción de políticas públicas como resultado de los ejercicios de investigación.

The disruptions and supply problems caused by the COVID-19 pandemic in the European Union (EU) have raised several issues concerning the EU’s participation in Global Value Chains (GVC). One of the ...

Since Alois Alzheimer described the pathology of Alzheimer's disease in 1907, an increasing number of studies have attempted to discover its causes and possible ways to treat it. For decades, research has focused on neuronal degeneration and the disruption to the neural circuits that occurs during disease progression, undervaluing in some extent the alterations to glial cells even though these alterations were described in the very first studies of this disease. In recent years, it has been recognized that different families of neuroglia are not merely support cells for neurons but rather key and active elements in the physiology and pathology of the nervous system. Alterations to different types of neuroglia (especially astroglia and microglia but also mature oligodendroglia and oligodendroglial progenitors) have been identified in the initial neuropathological changes that lead to dementia, suggesting that they may represent therapeutic targets to prevent neurodegeneration. In this review, based on our own studies and on the relevant scientific literature, we argue that a careful and in-depth study of glial cells will be fundamental to understanding the origin and progression of Alzheimer's disease. In addition, we analyze the main issues regarding the neuroprotective and neurotoxic role of neuroglial changes, reactions and/or involutions in both humans with Alzheimer's disease and in experimental models of this condition.

Copy Number Variation Regions (CNVR) were subjected to pedigree analysis to contribute to the understanding of their segregation patterns. Up to 492 Gochu Asturcelta pig individuals forming 478 different parents-offspring trios (61 different families) were genotyped using the Axiom_PigHDv1 Array (658,692 SNPs). CNVR calling, performed using two different platforms (PennCNV and QuantiSNP), allowed to identify a total of 344 candidate CNVR on the 18 porcine autosomes covering about 106.8 Mb of the pig genome. Sixty-nine CNVR were identified, to some extent, in both the parents and the offspring and were classified as segregating CNVR. The other candidate CNVR were called in one or more progeny but in neither parent and classified either as singleton or recurrent de novo CNVR. Segregating CNVR were, on average, larger and more frequent than the recurrent de novo CNVR (444.8 kb vs 287.9 kb long and 34 vs 5 individuals, respectively). In any case, segregating CNVR did not conform to strict Mendelian inheritance patterns: estimates of average paternal and maternal transmission rates ranged from 11.0 % to 13.4 % and mean inheritance rate was below 21 %. This issue should be carefully considered when interpreting the results of CNV studies. Segregating CNVR, present across generations, are unlikely to be artifacts or false positives and can be hypothesized to be important at the population level.

Background: Recently, the study of hydrolysates from food proteins has been increasingly due to their wide range of biological activities. Hydrolysates contain peptides of 2–20 amino acids that are inactive within the sequence of the parent protein, but, once released after proteolytic processes, they exert numerous beneficial health effects. Hemp, the non-drug variety of Cannabis sativa, is known as an important source of bioactive peptides due to the high quality of hempseeds protein (20–25%) and well-balanced amino acid profile. For this reason, during the last decade, numerous investigations have searched to elucidate the beneficial effects on the health of these hempseed protein hydrolysates. Scope and approach: The aim of this review was to collect all the scientific evidence on the demonstrated beneficial effects of hempseed protein hydrolysates (HHs). Key findings and conclusions: HHs have showed to possess antioxidant, immunomodulatory, hypotensive, hypoglycemic, and lipid-lowering capacities in vitro systems. All these effects have pointed out HHs as future ingredient for the development of functional foods or dietary supplements useful for the prevention of chronic diseases such as metabolic syndrome, diabetes or hypertension. However, few studies have evaluated the in vivo effects of HHs. For this reason, further studies carried out in animal models or human are necessary to better exploit the use of HHs for the development of new dietary supplements., I.C.-C. was supported by the VI Program of Inner Initiative for Re- search and Transfer of the University of Seville (VIPPIT-2020-II.4) and by a postdoctoral fellowship from the Andalusian Government Ministry of Economy, Knowledge, Business, and University (DOC_00587/2020). G.S.-S. was supported by a FPU grant from the Spanish Ministerio de Educación, Cultura y Deporte (FPU16/02339) and by an Erasmus+ Mobility Programme. A.I.A.-L. was funded by Andalusian Government Ministry of Health (PI-0136-2019). E.P.-E. was supported by the VI Pro- gram of Inner Initiative for Research and Transfer of University of Seville.

16 Páginas.-- 6 Figuras.-- 3 Tablas, Anxiety is the most prevalent psychiatric disorder worldwide, causing a substantial economic burden due to the associated healthcare costs. Given that commercial anxiolytic treatments may cause important side effects and have medical restrictions for prescription and high costs, the search for new natural and safer treatments is gaining attention. Since lupin protein hydrolysate (LPH) has been shown to be safe and exert anti-inflammatory and antioxidant effects, key risk factors for the anxiety process and memory impairment, we evaluated in this study the potential effects of LPH on anxiety and spatial memory in a Western diet (WD)-induced anxiety model in ApoE-/- mice. We showed that 20.86% of the 278 identified LPH peptides have biological activity related to anxiolytic/analgesic effects; the principal motifs found were the following: VPL, PGP, YL, and GQ. Moreover, 14 weeks of intragastrical LPH treatment (100 mg/kg) restored the WD-induced anxiety effects, reestablishing the anxiety levels observed in the standard diet (SD)-fed mice since they spent less time in the anxiety zones of the elevated plus maze (EPM). Furthermore, a significant increase in the number of head dips was recorded in LPH-treated mice, which indicates a greater exploration capacity and less fear due to lower levels of anxiety. Interestingly, the LPH group showed similar thigmotaxis, a well-established indicator of animal anxiety and fear, to the SD group, counteracting the WD effect. This is the first study to show that LPH treatment has anxiolytic effects, pointing to LPH as a potential component of future nutritional therapies in patients with anxiety., This research was funded by the Spanish Government, Ministerio de Economía y Competitividad (AGL2012-40247-C02-01, and AGL2012-40247-C02-02), the Andalusian Government Ministry of Health (PC-0111-2016-0111, and PEMP-0085-2020) and the PAIDI Program from the Andalusian Government (CTS160). G.S.-S. was supported by a FPU grant from the Spanish Ministerio de Educación, Cultura y Deporte (FPU16/02339). E.P.-E. and I.B. were supported by the VI Program of Inner Initiative for Research and Transfer of University of Seville (VI PPIT-US). I.C.-C. was supported by the VI Program of Inner Initiative for Research and Transfer of the University of Seville (VIPPIT-2020-II.4) and by a postdoctoral fellowship from the Andalusian Government Ministry of Economy, Knowledge, Business, and University (DOC_00587/2020). N.A.-S. was supported by a fellowship from the National Net RETICEF for Aging Studies (RD12/0043/0012 from the Instituto de Salud Carlos III, Spanish Ministerio de Ciencia e Innovación). A.I.Á.-L. was funded by Andalusian Government Ministry of Health (PI-0136-2019).

El presente artículo trata sobre el uso del video en el aula como estrategia de enseñanza en la asignatura de Ciencias Naturales, busca identificar el nivel de conocimiento que tienen los miembros de la comunidad educativa sobre dicho recurso, para generar una propuesta en donde se presenten algunas pautas a considerar para aplicar el video en la clase. La investigación es de tipo mixta, no experimental y de cohorte transversal. Gracias a la recolección de datos en donde participaron estudiantes, docentes y la rectora de la institución se pudo evidenciar su relación con el video, sus beneficios y limitaciones. Gracias a esta data se pudo conocer que tanto estudiantes como docente y rectora de la institución consideran que el video es un excelente recurso dentro del aula considerando que motiva y fomenta la interacción de quienes participan en la clase. Sin embargo, se pudo constatar que actualmente no existe una constante capacitación sobre el uso de mencionado recurso.

Se considera importante conocer las perspectivas que tienen los estudiantes frente al cambio de educación presencial a virtual durante la pandemia, con el propósito de generar recomendaciones, a las diferentes autoridades que representan al sistema educativo. Esta investigación es de carácter no experimental, con un paradigma epistemológico mixto, debido a que este proceso recoge, estudia y enlaza elementos cuantitativos y cualitativos dentro de un mismo estudio,su cohorte es transversal. En los resultados se evidencia que no todos los estudiantes tienen acceso al internet y no se conctan a las clases virtuakes, también se demuestra que los docentes deben mejorar las estrategias de enseñanza para obtener mejores niveles de satisfacción en los educandos; así mismo se menciona que los padres de familia o representantes en gran medida no ayudan a solventar las inquietudes educativas.