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291 results on '"Huang, Xianzhang"'

54. Traditional Chinese Medicine (TCM) Astragalus Membranaceus and Curcuma Wenyujin Promote Vascular Normalization in Tumor-derived Endothelial Cells of Human Hepatocellular Carcinoma

Author

Zhijian Yang, Decai Tang, Robert M. Hoffman, Chaoyun Zhang, Hua Bian, Gang Yin, L I Han, Wenhua Zang, Hao Pengfei, Ding Shengchen, Y U Sun, and Huang Xianzhang

Subjects
Tube formation, Cancer Research, biology, Chemistry, education, Immunocytochemistry, CD34, General Medicine, medicine.disease, biology.organism_classification, 03 medical and health sciences, chemistry.chemical_compound, Astragalus, 0302 clinical medicine, HIF1A, Oncology, Cell culture, 030220 oncology & carcinogenesis, Hepatocellular carcinoma, medicine, Cancer research, Nintedanib
Abstract

Background/aim The aim of the present study was to investigate the vascular normalization effect of traditional Chinese medicine Astragalus membranaceus (AM) and Curcuma wenyujin (CW) on tumor-derived endothelial cells (TECs). Materials and methods TECs were isolated from the xenografted HCC cell line HepG2 expressing red fluorescent protein (RFP). The effect of AM and CW on TECs proliferation was measured using the CCK8 assay. The vascular normalization potential of AM and CW was assessed using a tube formation assay. Immunocytochemistry was performed to assess the effect of AM and CW on the expression of angiogenic maker CD34 and hypoxia-inducible factor HIF1a. Results The isolated TECs and endothelioma (EOMA) cells did not differ with regard to the expression levels of endothelial markers CD34, VEGFR-1, VEGFR-2, PDGFR-α and PDGFR-β. All AM, CW, AM+CW and Nintedanib (Nin) showed a dose-dependent increasing inhibition effect on either TECs or EOMA cells. AM, CW and AM+CW significantly reduced HIF1a expression, increased CD34 expression and enhanced endothelial network formation in TECs or EOMA cells compared to the control. Conclusion AM and CW promoted vascular normalization in tumor-derived endothelial cells of HCC, through increased expression of CD34 and reduced expression of HIF1a.

Published
2019

56. MIL-1, a novel antitumor agent derived from natural product millepachine, acts as tubulin polymerization inhibitor for the treatment of hepatocellular carcinoma

Author

Yan, Jun, primary, Zhuang, Qizhen, additional, Li, Zhenzhen, additional, Xiong, Yujuan, additional, He, Min, additional, Kang, Cunmin, additional, Zhang, Qiaoxuan, additional, Han, Liqiao, additional, Liang, Enyu, additional, Liu, Hongcan, additional, Ke, Peifeng, additional, and Huang, Xianzhang, additional

Published
2021
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61. Using Quasispecies Patterns of Hepatitis B Virus to Predict Hepatocellular Carcinoma With Deep Sequencing and Machine Learning

Author

Chen, Shipeng, primary, Zhang, Zihan, additional, Wang, Ying, additional, Fang, Meng, additional, Zhou, Jun, additional, Li, Ya, additional, Dai, Erhei, additional, Feng, Zhaolei, additional, Wang, Hao, additional, Yang, Zaixing, additional, Li, Yongwei, additional, Huang, Xianzhang, additional, Jia, Jian’an, additional, Li, Shuang, additional, Huang, Chenjun, additional, Tong, Lin, additional, Xiao, Xiao, additional, He, Yutong, additional, Duan, Yong, additional, Zhu, Shanfeng, additional, and Gao, Chunfang, additional

Published
2020
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65. Prognostic value of serum soluble interleukin‐23 receptor and related T‐helper 17 cell cytokines in non‐small cell lung carcinoma

Author

Liu, Dan, primary, Xing, Shan, additional, Wang, Wan, additional, Huang, Xianzhang, additional, Lin, Haibiao, additional, Chen, Yonghe, additional, Lan, Kai, additional, Chen, Lin, additional, Luo, Fudong, additional, Qin, Sheng, additional, Liang, Rongliang, additional, Bai, Caiying, additional, Xu, Jianhua, additional, and Liu, Wanli, additional

Published
2020
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66. Quasispecies Pattern of Hepatitis B Virus Predicts Hepatocellular Carcinoma Using Deep-Sequencing and Machine Learning

Author

Chen, Shipeng, primary, Zhang, Zihan, additional, Wang, Ying, additional, Fang, Meng, additional, Zhou, Jun, additional, Li, Ya, additional, Dai, Erhei, additional, Feng, Zhaolei, additional, Wang, Hao, additional, Yang, Zaixing, additional, Li, Yongwei, additional, Huang, Xianzhang, additional, Jia, Jianan, additional, Li, Shuang, additional, Huang, Chenjun, additional, Tong, Lin, additional, Xiao, Xiao, additional, He, Yutong, additional, Duan, Yong, additional, Zhu, Shanfeng, additional, and Gao, Chunfang, additional

Published
2020
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68. A simple and reliable measurement procedure for determination of glycocholic acid in human serum by isotope-dilution liquid chromatography-tandem mass spectrometry

Author

Yuzhu Xu, Huang Xianzhang, Min Zhan, Hongcan Liu, Jun Yan, Liqiao Han, Peifeng Ke, Ahui Wang, Yangfen Ou, and Qiaoxuan Zhang

Subjects
Detection limit, Chromatography, 010401 analytical chemistry, Glycocholic acid, Isotope dilution, 010402 general chemistry, Condensed Matter Physics, Mass spectrometry, 01 natural sciences, 0104 chemical sciences, chemistry.chemical_compound, chemistry, Liquid chromatography–mass spectrometry, Calibration, Gravimetric analysis, Sample preparation, Physical and Theoretical Chemistry, Instrumentation, Spectroscopy
Abstract

Glycocholic acid (GCA) is an important identified biomarker for various hepatobiliary diseases. However, results obtained from routine clinical measurement significantly varied due to the interference from the extremely similar structure analogues, which cause confusion to clinical diagnosis. In this study, a bracketing calibration-based isotope dilution liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) method was developed. The established method exhibited good performance criteria through the adoption of isotope dilution, calibrator bracketing, and gravimetric measurements. The satisfactory precision (0.16%–2.01%) and good recoveries were achieved at five spiked levels (98.0–100.9%). The limit of detection (LOD) and limit of quantification (LOQ) were 0.01 ng/mL and 0.05 ng/mL, respectively. No interference, matrix effect, and carryover were observed. The sample preparation process was easier to operate, without further solvent evaporation and time-saving. The optimized method was successfully applied to quantify GCA with a wide range of concentrations in clinical human serum samples and also compared with clinical routine systems.

Published
2021

70. Using Quasispecies Patterns of Hepatitis B Virus to Predict Hepatocellular Carcinoma With Deep Sequencing and Machine Learning.

Author

Chen, Shipeng, Zhang, Zihan, Wang, Ying, Fang, Meng, Zhou, Jun, Li, Ya, Dai, Erhei, Feng, Zhaolei, Wang, Hao, Yang, Zaixing, Li, Yongwei, Huang, Xianzhang, Jia, Jian'an, Li, Shuang, Huang, Chenjun, Tong, Lin, Xiao, Xiao, He, Yutong, Duan, Yong, and Zhu, Shanfeng

Subjects
HEPATITIS B virus, HEPATOCELLULAR carcinoma, MACHINE learning, RECEIVER operating characteristic curves, CHRONIC hepatitis B, RESEARCH, LIVER tumors, SEQUENCE analysis, RESEARCH methodology, HEPATITIS viruses, EVALUATION research, COMPARATIVE studies, TRANSFERASES
Abstract

Background: Hepatitis B virus (HBV) infection is one of the main leading causes of hepatocellular carcinoma (HCC) worldwide. However, it remains uncertain how the reverse-transcriptase (rt) gene contributes to HCC progression.Methods: We enrolled a total of 307 patients with chronic hepatitis B (CHB) and 237 with HBV-related HCC from 13 medical centers. Sequence features comprised multidimensional attributes of rt nucleic acid and rt/s amino acid sequences. Machine-learning models were used to establish HCC predictive algorithms. Model performances were tested in the training and independent validation cohorts using receiver operating characteristic curves and calibration plots.Results: A random forest (RF) model based on combined metrics (10 features) demonstrated the best predictive performances in both cross and independent validation (AUC, 0.96; accuracy, 0.90), irrespective of HBV genotypes and sequencing depth. Moreover, HCC risk scores for individuals obtained from the RF model (AUC, 0.966; 95% confidence interval, .922-.989) outperformed α-fetoprotein (0.713; .632-.784) in distinguishing between patients with HCC and those with CHB.Conclusions: Our study provides evidence for the first time that HBV rt sequences contain vital HBV quasispecies features in predicting HCC. Integrating deep sequencing with feature extraction and machine-learning models benefits the longitudinal surveillance of CHB and HCC risk assessment. [ABSTRACT FROM AUTHOR]

Published
2021
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71. Traditional Chinese Medicine (TCM) Astragalus Membranaceus and Curcuma Wenyujin Promote Vascular Normalization in Tumor-derived Endothelial Cells of Human Hepatocellular Carcinoma

Author

ZANG, WENHUA, primary, BIAN, HUA, additional, HUANG, XIANZHANG, additional, YIN, GANG, additional, ZHANG, CHAOYUN, additional, HAN, LI, additional, HAO, PENGFEI, additional, DING, SHENGCHEN, additional, SUN, YU, additional, YANG, ZHIJIAN, additional, HOFFMAN, ROBERT M., additional, and TANG, DECAI, additional

Published
2019
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75. Cancer patients' attitudes towards Chinese medicine: a Hong Kong survey

Author

Law Chun-key, Peng Heng, Cheng Chung-wah, Lam Yuen-chi, Huang Xianzhang, and Bian Zhaoxiang

Subjects
Other systems of medicine, RZ201-999
Abstract

Abstract Background This article reports a survey conducted in Hong Kong on the cancer patients' attitudes towards Chinese medicine treatment. Methods Cancer patients from three Chinese medicine clinics and one oncology clinic were interviewed with a structured questionnaire. Results Of a total of 786 participants included in the study, 42.9% used Western medicine only; 57.1% used at least one form of Chinese medicine; 5 participants used Chinese medicine only; and 56.5% used Chinese medicine before/during/after Western medicine treatment. Commonly used Western medicine and Chinese medicine treatments included chemotherapy (63.7%), radiotherapy (62.0%), surgery (57.6%), Chinese herbal medicine (53.9%) and Chinese dietary therapy (9.5%). Participants receiving chemotherapy used Chinese medicine (63.3%) more than those receiving any other Western medicine treatments. Spearman correlation coefficients showed that the selection of Chinese medicine was associated with the cancer type (rs = -1.36; P < 0.001), stage (rs = 0.178; P < 0.001), duration (rs = -0.074; P = 0.037), whether receiving chemotherapy (rs = 0.165; P < 0.001) and palliative therapy (rs = 0.087; P = 0.015). Nearly two-thirds of the participants (N = 274) did not tell their physicians about using Chinese medicine. Over two-thirds of all participants (68.2%) believed that integrated Chinese and Western medicine was effective. Conclusion Chinese medicine is commonly used among Hong Kong cancer patients. The interviewed cancer patients in Hong Kong considered integrative Chinese and Western medicine is an effective cancer treatment.

Published
2009
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76. Investigation on Reference Intervals and Regional Differences of Platelet Indices in Healthy Chinese Han Adults

Author

Zhang Xin, Huang Xianzhang, Guo Wei, Chen Wenxiang, Wu Xinzhong, Wang Lanlan, Jiang Hong, Hao Xiao-ke, Zhang Jie, Ma YueYun, Peng Mingting, Pan Baishen, Shang Hong, Zhao Min, and Qiao Rui

Subjects
Microbiology (medical), medicine.medical_specialty, Platelet indices, business.industry, Biochemistry (medical), Clinical Biochemistry, Platelet Distribution Width, Public Health, Environmental and Occupational Health, Hematology, Reference intervals, Medical Laboratory Technology, Chinese han population, Reference values, Internal medicine, Immunology and Allergy, Medicine, Mean platelet volume, Chinese han, business, Regional differences
Abstract

Background Reference intervals are important for interpretation of clinical laboratory tests. The platelet (PLT) indices such as the mean platelet volume (MPV) and platelet distribution width (PDW) are newer hematological parameters, which have been recently reported as clinically valuable biomarkers. However, there are not many studies that have estimated the reference intervals for these parameters in healthy Chinese Han adults. Objective The objectives of this study were to establish reference values of PLT indices [including PLT count, MPV, PDW, platelet-large cell ratio (P-LCR), and plateletcrit (Pct)] for healthy Chinese Han adults. We also aimed to determine the region-based differences of PLT indices in China. Methods A total of 4,642 volunteers with a mean age of 43 were recruited from six regions of China. PLT indices were performed on Sysmex XE-2100 hematology analyzers, whose traceability was well verified. Results There were significant region-based differences for all PLT indices. Reference people in Chengdu had the lowest mean PLT count and Pct, but the highest MPV, PDW, and P-LCR among the six regions. Therefore, we derived the reference intervals in Chinese Han population excluding Chengdu reference people for PLT indices as PLT count: (127–341) × 109/l; MPV: (9.20–13.30) fl; PDW: 9.90–19.00%; P-LCR: 18.10–52.00%; Pct: 16.00–41.00%. Conclusions Region-specific reference intervals are essential as there were statistically significant region-related differences in the PLT parameters. The reference intervals established in this study differed from the existing reference values. Chengdu region may need proper specific reference ranges, which apply to their people, for all PLT parameters.

Published
2014

82. Evaluation of the Elecsys syphilis immunoassay for routine screening of serum samples in China

Author

Tao, Chuanmin, primary, Hao, Xiaoke, additional, Xu, Wei, additional, Zhang, Jie, additional, Pan, Shiyang, additional, Tao, Zhihua, additional, Li, Xiaofei, additional, Chen, Junmei, additional, Zhang, Bingchang, additional, Qiu, Yurong, additional, Wu, Yanan, additional, Ou, Qishui, additional, Huang, Xianzhang, additional, and Wang, Lanlan, additional

Published
2017
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83. The diagnostic value of serum creatinine and cystatin c in evaluating glomerular filtration rate in patients with chronic kidney disease: a systematic literature review and meta-analysis

Author

Qiu, Xilian, primary, Liu, Chunyong, additional, Ye, Yuqiu, additional, Li, Huiqun, additional, Chen, Yanbing, additional, Fu, Yongmei, additional, Liu, Zhenjie, additional, Huang, Xianzhang, additional, Zhang, Yunqiang, additional, Liao, Xueyuan, additional, Liu, Hongyong, additional, Zhao, Wenbo, additional, and Liu, Xun, additional

Published
2017
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86. PICT-1 is a key nucleolar sensor in DNA damage response signaling that regulates apoptosis through the RPL11-MDM2-p53 pathway

Author

Chen, Hongbo, primary, Han, Liqiao, additional, Tsai, Hsiangi, additional, Wang, Zhiwei, additional, Wu, Yanping, additional, Duo, Yanhong, additional, Cao, Wei, additional, Chen, Lijun, additional, Tan, Zhirong, additional, Xu, Ning, additional, Huang, Xianzhang, additional, Zhuang, Junhua, additional, and Huang, Laiqiang, additional

Published
2016
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87. Development of reference intervals for serum alkaline phosphatase among adults in Southern China traced to the new IFCC reference measurement procedure

Author

Zemin Wan, Jianbing Wang, Junhua Zhuang, Xiaobin Wu, Liqiao Han, Huang Xianzhang, Ruili Zeng, Peifeng Ke, Qiaoxuan Zhang, and Haibiao Lin

Subjects
Adult, Male, 030213 general clinical medicine, medicine.medical_specialty, China, Adolescent, Clinical Biochemistry, Physiology, 01 natural sciences, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Reference Values, Internal medicine, medicine, Humans, Volunteer, Serum alkaline phosphatase, Aged, Chemistry, 010401 analytical chemistry, Biochemistry (medical), General Medicine, Clinical Enzyme Tests, Middle Aged, Alkaline Phosphatase, 0104 chemical sciences, Reference intervals, Endocrinology, Southern china, Reference measurement, Reference values, Cohort, Alkaline phosphatase, Female
Abstract

Serum alkaline phosphatase (ALP) plays a critical role in the diagnosis of various diseases, and the establishment of relevant, reliable reference intervals (RI) is key to avoiding misdiagnoses. In 2011, IFCC published the new reference measurement procedure (RMP) for the determination of serum ALP in which one of the main modifications was the measuring temperature of the assay. Here, the new RMP was used to help establish RIs for serum ALP concentrations in healthy Chinese Han.Volunteer individuals in Guangdong province, China (n=1622) were screened by questionnaire and laboratory testing for eligibility as a reference. Blood (20 mL) was collected and samples were measured by the Roche Modular system using the new RMP for the serum ALP compatible method. Partitioning of values by gender and/or age was evaluated with a standard normal deviate test after removing outliers. A simple non-parametric method for a two-sided 95% distribution of reference values was calculated.Serum ALP concentrations were obtained from the cohort of eligible reference individuals (n=658). The RI for serum ALP in males age 18–79 years was 48–131 U/L. Females were partitioned into two age groups based on statistical analysis, 18–49 years and 50–79 years, and the RIs derived were 40–106 U/L and 57–159 U/L, respectively.RIs for serum ALP for Chinese Han individuals in between the ages of 18 and 79 years were determined and required partitioning due to the higher ALP values of females age 50–79 years.

Published
2015

89. A comparative evaluation of the analytical performances of Capillarys 2 Flex Piercing, Tosoh HLC-723 G8, Premier Hb9210, and Roche Cobas c501 Tina-quant Gen 2 analyzers for HbA1c determination

Author

Wu, Xiaobin, primary, Chao, Yan, additional, Wan, Zemin, additional, Wang, Yunxiu, additional, Ma, Yan, additional, Ke, Peifeng, additional, Wu, Xinzhong, additional, Xu, Jianhua, additional, Zhuang, Junhua, additional, and Huang, Xianzhang, additional

Published
2016
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91. Elevated circulating CD14lowCD16+ monocyte subset in primary biliary cirrhosis correlates with liver injury and promotes Th1 polarization

Author

Peng, Anping, primary, Ke, Peifeng, additional, Zhao, Rong, additional, Lu, Xinyi, additional, Zhang, Cheng, additional, Huang, Xianzhang, additional, Tian, Guangjun, additional, Huang, Jun, additional, Wang, Jinli, additional, Invernizzi, Pietro, additional, Chen, Qubo, additional, and Zhuang, Junhua, additional

Published
2015
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92. Complete Blood Count Reference Intervals for Healthy Han Chinese Adults

Author

Wu, Xinzhong, primary, Zhao, Min, additional, Pan, Baishen, additional, Zhang, Jie, additional, Peng, Mingting, additional, Wang, Lanlan, additional, Hao, Xiaoke, additional, Huang, Xianzhang, additional, Mu, Runqing, additional, Guo, Wei, additional, Qiao, Rui, additional, Chen, Wenxiang, additional, Jiang, Hong, additional, Ma, Yueyun, additional, and Shang, Hong, additional

Published
2015
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95. Identification of haemoglobin New York by haemoglobin A1c measurement using the Sebia Capillarys 2 Flex Piercing system.

Author

Chao, Yan, Wan, Zemin, Wu, Xiaobin, Qiu, Feng, Wu, Xinzhong, Wang, Yunxiu, Ke, Peifeng, Xu, Jianhua, Zhuang, Junhua, and Huang, Xianzhang

Subjects
HEMOGLOBINOPATHY, HEMOGLOBINS, ION exchange (Chemistry), HIGH performance liquid chromatography, IMMUNOASSAY, PATHOLOGICAL laboratories
Abstract

Haemoglobinopathies may interfere with the haemoglobin A1c (HbA1c) measurement, leading to incorrect diagnosis and inappropriate treatment. It is essential that HbA1c assays are capable of identifying haemoglobinopathies. We report two cases of haemoglobin New York (HbNY) discovered through HbA1c analysis using capillary electrophoresis (Capillarys 2 Flex Piercing [C2FP], Sebia). We used these samples to evaluate the ability of three other HbA1c assays to identify this variant: ion-exchange high-performance liquid chromatography (Variant II Turbo [VII-T], Bio-Rad); boronate affinity high-performance liquid chromatography (Ultra2, Trinity Biotech) and immunoassay (Cobas c501 Tina-quant Generation 3, Roche Diagnostics). Each method was used for HbA1c assay of in samples from two cases of heterozygous haemoglobinopathy: β0-thalassemia/HbNY (Case 1) and HbA/NY (Case 2). Only the C2FP system detected HbNY (an additional peak appeared between HbA1c and HbA0). Clinical laboratories should be aware of the limitations of their HbA1c assay methods especially in geographic areas, where haemoglobinopathy prevalence is high. [ABSTRACT FROM AUTHOR]

Published
2017
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97. Reference Intervals of Serum Sodium, Potassium, and Chlorine in Chinese Han Population and Comparison of Two ISE Methods

Author

Jia, Keke, primary, Zhang, Chuanbao, additional, Huang, Xianzhang, additional, Wang, Lanlan, additional, Hao, Xiaoke, additional, Mu, Runqing, additional, Pan, Baishen, additional, Zhang, Jie, additional, Chen, Wenxiang, additional, Xu, Ning, additional, Li, Guixing, additional, Ma, Yueyun, additional, Ma, Ming, additional, Guo, Wei, additional, and Shang, Hong, additional

Published
2014
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99. First Definition of Reference Intervals of Liver Function Tests in China: A Large-Population-Based Multi-Center Study about Healthy Adults

Author

Mu, Runqing, primary, Chen, Wenxiang, additional, Pan, Baishen, additional, Wang, Lanlan, additional, Hao, Xiaoke, additional, Huang, Xianzhang, additional, Qiao, Rui, additional, Zhao, Min, additional, Zhang, Chuanbao, additional, Guo, Wei, additional, Huang, Hengjian, additional, Ma, Yueyun, additional, Zhuang, Junhua, additional, Zhang, Jie, additional, and Shang, Hong, additional

Published
2013
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