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Polytetrafluoroethylene (PTFE) with good resistance can be used under extreme conditions, however, its surface hydrophobicity greatly limits its wide application in water treatment field. In this work, the hydrophobic PTFE microfiltration (MF) membrane was innovatively modified into a hydrophilic loose nanofiltration (NF) via synergic effect of the deposited polyethyleneimine (PEI) and poly(vinylpyrrolidone) (PVP) with assistance of fluorocarbon surfactant (FCS), and then crosslinked by glutaraldehyde (GA). The surface morphology, porosity and aperture size of the obtained cPEI-PVP-FCS/PTFE (cPPF) membrane were characterized in detail. The resultant membrane showed good dye desalination performance with high Evans blue rejection of 98.5%, NaCl rejection of 13.4%, along with permeance of 192.1 L m−2 h−1·MPa−1. In addition, the membrane showed long-term stability and good acid resistance.

Resilient city is an ideal goal and model of urban development proposed in response to today’s complex and dynamic environmental changes. In this study, a resilient city evaluation framework of “social resilience-economic resilience-urban infrastructure and service-urban governance” was built upon the multi-dimensionality of the urban system; the entropy weight method was used to measure the level of urban resilience in Hunan Province while an obstacle degree model was used to identify any obstacle factor restricting to the development of resilience. The results show that the level of urban resilience in Hunan Province has grown slowly over the past 10 years, and there is an obvious regional difference in it. There are more and more highly resilient cities, but medium/low-resilience cities still dominate the province, forming a spatial process of evolution from “medium/high-level dispersion” to “medium/high-level aggregation” in the Changsha-Zhuzhou-Xiangtan Urban Agglomeration. The level of urban resilience is predominantly hindered by the social and economic systems; at the index layer, most obstacle factors are moving from the economic system to the social-economic-urban infrastructure and service system.

In this paper, a two-loop photoelectric oscillator based on chirped fiber Bragg grating (CFBG) is used to construct a swept source, which acts on the frequency scanning array antenna to realise multibeamforming. The simulation results of the designed beamforming system have shown that it can realise wide-range beam scanning and has ultralow phase noise.

Background: Genetic mutations are quite common in non-small cell lung cancer (NSCLC), however, their prognostic value remains controversial. Methods: This study explored the mutational landscape of tumor samples from patients with advanced NSCLC by next-generation sequencing (NGS). A total of 101 NSCLC patients in stage III or IV receiving first-line treatment were included. Results: TP53 mutation was the most frequent genetic alteration in NSCLC tumors (68%), followed by EGFR (49%), CDKN2A (12%), LRP1B (9%), and FAT3 (9%) mutations. Among 85 patients with stage IV NSCLC, first-line targeted therapy remarkably prolonged progression-free survival (PFS) of patients compared with first-line chemotherapy (p = 0.0028). Among 65 patients with stage IV NSCLC whose tumors harbored EGFR, ALK, ROS, or BRAF mutations, first-line targeted therapy substantially prolonged the PFS of patients (p = 0.0027). In patients with TP53 mutations who received first-line targeted therapy or chemotherapy, missense mutation was the most common mutation type (36/78), and exon 5 represented the most common mutated site (16/78). Conclusions: TP53 mutation in exon 5 could independently predict poor PFS of patients with stage IV NSCLC after the first- line treatment. Moreover, mutations in TP53 exon 5 and LRP1B were associated with shorter PFS of such patients whether after first-line chemotherapy or targeted therapy, respectively. Thus, these patients should be given immunotherapy or immunochemotherapy.

The utilization of low-N tolerant and N-efficifent varieties offers significant benefits in terms of reducing the need for excessive N fertilizer input. Quinoa, a resilient crop for agroecological transition, possesses a wide genetic diversity, making it suitable for selecting genotypes that require less N fertilizer. In this study, the growth and physiological characteristics of nine quinoa genotypes were assessed to determine their low-N tolerance using the fuzzy membership function. Based on comprehensive evaluation indices, three genotypes were identified: low-N tolerant (BL22), intermediately tolerant (A29), and sensitive (G68). These genotypes were exposed to varying N concentrations, including normal (4 mM), low (0.8 mM), and deficient N (no N) conditions. The results indicate that low-N conditions altered root phenotype, with reduced biomass, total protein, and chlorophyll content; increased soluble sugar levels; and inhibited N-metabolizing enzymes (nitrate reductase, glutamine synthetase, glutamate synthase) and N uptake. Under low-N conditions, the tolerant genotype exhibited higher maximal efficiency of photosystem II (Fv/Fm), root vitality, and N content compared to the sensitive genotype. Interestingly, the sensitive genotype displayed elongated and thinner shoots and roots in response to low-N, suggesting that plant height and root length are unreliable indicators of low-N tolerance in quinoa. In contrast, shoot and root dry biomass, Fv/Fm, chlorophyll content, N-metabolizing enzymes, and N content proved to be reliable indicators of low-N tolerance during the early growth stage of quinoa. Overall, these findings highlight the potential of utilizing specific growth and physiological parameters as indicators for screening low-N tolerant quinoa genotypes, thereby reducing dependence on N fertilizers.

Abstract Background The aim was to determine the potential association of the gut microbiota composition, especially the abundance of Actinobacteria, as well as the differentiation of functional and resistance genes with age (young adults vs elderly subjects) in China. Results The patterns of relative abundance of all bacteria isolated from fecal samples differed between young adults and elderly subjects, but the alpha diversity (Chao1 P = 0.370, Shannon P = 0.560 and Simpson P = 0.270) and beta diversity (ANOSIM R = 0.031, P = 0.226) were not significantly different. There were 3 Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways (carbon metabolism, inositol phosphate metabolism, and sesquiterpenoid and triterpenoid biosynthesis) and 7 antibiotic resistant genes (ARGs) (macrolide lincosamide-streptogramin B (MLSB), tetracycline, aminoglycoside, sulfonamide, fosmidomycin, lincomycin, and vancomycin) that showed significant differences between the 2 groups (all P 0.05). Only the Bifidobacterium_dentium species showed significant differences between the 2 groups (P = 0.013), with a higher abundance in elderly subjects but absent in young adults. Conclusions The present study revealed that there were 3 KEGG metabolic pathways and 7 ARGs as well as enhanced Bifidobacterium_dentium species abundance in elderly compared to young subjects.

In China, highway port networks are essential in carrying out tractor and semitrailer transportation operations. To analyze the characteristics of tractor and semitrailer routing in highway port networks, this study examined the situation in which the demands at both ends of the operation might be unbalanced and multiple requirements might be raised in the operation of tractor and semitrailer transportation. An optimal tractor and semitrailer routing model for an entire network was established to reduce the total transportation costs and the number of towing vehicles in the network. Moreover, a heuristic algorithm was designed to solve the model. The comparisons of Strategy 1 and Strategy 2 for a two-stage network swap trailer show that the number of pure network swaps trailer tractors decreases by 21.6% and 18.6%, respectively; and that the cost drops by 7.8% and 7.9%, respectively. In other words, swap trailer transport enterprises can abandon the original swap trailer transportation mode for a two-stage network and adopt a routing optimization strategy for an entire network to achieve superior operation performance, reduce costs, and enhance profits. The study provides a reference for optimizing tractor and semitrailer routing in highway port networks with balanced and multiple demands.

Abstract Background Idiopathic multicentric Castleman disease (iMCD) is an uncommon lymphoproliferative disorder and lacks treatment consensus. Herein, we report a case of iMCD complicated with Sjögren’s syndrome (SS) and secondary membranous nephropathy (SMN). Case presentation A 45-year-old female with dry mouth for 3 months and anasarca and proteinuria for 2 months was admitted. She also experienced chest tightness, wheezing, fever, weight loss, moderate proteinuria and hypoalbuminemia. A computed tomography (CT) scan revealed a tissue mass in the thymus area and enlarged multiple lymph nodes. Her symptoms did not improve after resection of the thymus mass. The pathological findings were “reactive hyperplasia of the mediastinal lymph nodes and thymic hyperplasia”. Lymph node biopsy findings confirmed iMCD with human herpes virus-8 (HHV-8) negativity. Based on anti-nuclear antibody (ANA) 1:320, anti-SSA and anti-SSB antibody positivity, salivary flow less than 0.1 ml/min and lip biopsy with focal lymphocytic sialadenitis, SS was diagnosed. Kidney biopsy showed secondary membranous nephropathy with endocapillary cell proliferation and infiltration of plasma cells and lymphocytes in the tubulointerstitium. Serum interleukin-6 (IL-6) levels were significantly increased, and therapy with tocilizumab (anti-IL-6 receptor antibody) worked well. The combination of cyclophosphamide (CyS) with methylprednisolone (MP) maintained satisfactory remission. Conclusions Our case of iMCD with SS and SMN is rare. There is a need for increased awareness of the disease to avoid unnecessary procedures and misdiagnoses. IL-6 was extremely high, and there was a rapid response to anti-IL-6 receptor agents. The combination of CyS with MP maintained complete remission.

Objectives: To compare the performance of two syndromic panels: Luminex xTAG Gastrointestinal Pathogen Panel (GPP) and FilmArray Gastrointestinal (GI) panel. Methods: A total of 243 diarrhea specimens were detected by two panels in parallel, and the inconsistent results were analyzed by real-time PCR or reverse transcription PCR (RT-PCR). The target concentration in specimens was examined by comparing the crossing point values of FilmArray, the median fluorescence intensity of xTAG and the cycle threshold values in any discrepancies. Results: For pathogens detected by both panels, the positive rates of FilmArray GI and xTAG GPP were 65.0% and 48.6%, respectively. The two panels showed high consistency (kappa ≥0.74) in detecting norovirus, rotavirus and Campylobacter, while there was low consistency (kappa ≤0.40) in detecting Cryptosporidium, Salmonella, Shiga toxin-producing Escherichia coli (STEC) and enterotoxigenic Escherichia coli (ETEC). Samples with low concentration targets were more often detected by FilmArray than with xTAG GPP. The xTAG GPP was more likely to be affected by amplification inhibitors. Several defects of xTAG GPP were found in detecting ETEC. Conclusions: FilmArray was more sensitive. For specimens with low target concentrations or containing ETEC heat stable enterotoxin, the false negatives of xTAG GPP need to be considered.

Membrane filtration technology has attracted extensive attention in academia and industry due to its advantages of eco-friendliness related to environmental protection and high efficiency. Polyamide thin-film composite nanofiltration (PA TFC NF) membranes have been widely used due to their high separation performance. Non-acid-resistant PA TFC NF membranes face tremendous challenges in an acidic environment. Novel and relatively acid-resistant polysulfonamide-based and triazine-based TFC NF membranes have been developed, but these have a serious trade-off in terms of permeability and selectivity. Hence, how to improve acid resistance of TFC NF membranes and their separation performance in acidic environments is a pivotal issue for the design and preparation of these membranes. This review first highlights current strategies for improving the acid resistance of PA TFC NF membranes by regulating the composition and structure of the separation layer of the membrane performed by manipulating and optimizing the construction method and then summarizes the separation performances of these acid-resistant TFC NF membranes in acidic environments, as studied in recent years.

In order to analyze the influence of key additives during processing on the flavor of infant formula, the headspace-gas chromatography-ion mobility spectrometry, electronic tongue, and electronic nose techniques were used to evaluate flavor during the processing of stage 1 infant formula milk powder (0–6 months), including the analysis of seven critical additives. A total of 41 volatile compounds were identified, involving 12 aldehydes, 11 ketones, 9 esters, 4 olefins, 2 alcohols, 2 furans, and 1 acid. The electronic nose metal oxide sensor W5S had the highest response, followed by W1S and W2S, illustrating that these three sensors had great effects on distinguishing samples. The response results of the electronic tongue showed that the three sensory attributes of bitter, salty, and umami, as well as the richness of aftertaste, were more prominent, which contributed significantly to evaluating the taste profile and distinguishing among samples. Raw milk is an essential control point in the flavor formation process of stage 1 infant formula milk powder. Demineralized whey powder is the primary source of potential off-flavor components in hydrolyzed milk protein infant formula. This study revealed the quality characteristics and flavor differences of key additives in the production process of stage 1 infant formula milk powder, which could provide theoretical guidance for the quality control and sensory improvement of the industrialized production of infant formula.

To explore the efficiency of selenium (Se) fertilizer application in dryland maize, we tested five Se fertilizer application treatments: 0 g ha−1 (Se0), 75 g ha−1 (Se1), 150 g ha−1 (Se2), 225 g ha−1 (Se3), and 300 g ha−1 (Se4). Compared with Se0, Se2 increased the leaf area, chlorophyll content, internode length, plant height, and ear height by 7.95%, 3.20%, 13.19%, 1.89%, and 7.98%, respectively. Se2 and Se3 significantly affected the stem internode diameter, cortex thickness, and cellulose content, which were positively correlated with lodging resistance. Compared with Se0, Se3 promoted the contents of soluble sugar, crude protein, crude fat, and starch in grains, which increased by 9.48%, 6.59%, 1.56%, and 4.82%, respectively. It implies that maize grain application of Se significantly improves their Se content. Se1 did not observably influence the growth of maize, and the promoting effect of Se4 on maize decreased. The lodging resistance of maize as analyzed by Pearson correlation analysis correlated with the application of Se fertilizer. It proved that higher yield, grain quality, grain Se content, and lodging resistance of stems were concerned with Se fertilizer application in the range of 150–225 g ha−1. The results provide useful information for Se fertilizer treatment in dryland maize.

Background: Associations between metabolic syndrome (MetS) and osteoporotic fracture have been reported. However, the epidemiological studies are not conclusive. The objective of the study was to determine whether metabolic syndrome associates with osteoporotic fracture. Methods: This was a cross-sectional study of 9930 Chinese adults aged 40 year or older in the Chongming District, Shanghai, China. A questionnaire, anthropometric measurements and laboratory tests were conducted. MetS was defined according to the updated National Cholesterol Education Program Adult Treatment Panel III criteria for Asian-Americans. A history of fractures was collected with an interviewer-assisted questionnaire. Osteoporotic fractures were defined as fractures that occurred due to low-trauma in 2 years prior to the study. Results: Among women, the prevalence of osteoporotic fractures was significantly higher in those with MetS (3.5 vs. 2.6 %, P =0.028). However, the difference was not found in men (2.6 vs. 2.4 %, P =0.737). The presence of Mets was significantly associated with increased odds of osteoporotic fracture among women (odds ratio 1.22; 95 % confidence interval 1.12-1.54; P = 0.039) after controlling for potential confounders. The significant associations were not detected in men. Conclusions: The presence of MetS was significantly associated with a recent history of osteoporotic fracture in middle-aged and elderly Chinese women. [ABSTRACT FROM AUTHOR]

Due to the random volatility, a large amount of renewable energy will bring challenges to the security and stability of distribution network. Comprehensive consideration of system economics, security and flexibility has become the focus of research on distribution network optimization planning under the new situation. For the flexible resource allocation problem of distribution network, this paper analyzes the supply and demand relationship of the flexibility of the distribution network, and establishes a bi-level operation-planning joint optimization model for flexible resources. In the operational layer, we not only introduce the insufficient flexibility rate as the evaluation index of system flexibility, but also introduce the network loss, the abandoned wind and solar energy in the economic penalty, aiming at optimizing the annual operating cost of the system. In the planning layer, we evaluate the system security by introducing the comprehensive security index of the system, aiming at optimizing the annual comprehensive cost of the system. In addition, this paper also considers the third-party companies' investment in energy storage system in the electricity market, and further analyses the impact of energy storage operation strategies under different investors on flexible resource allocation. In this paper, the particle swarm optimization algorithm is used to solve the bi-level allocation model. Finally, the IEEE 33-bus test system is used for verification and analysis of the case. The results verify the validity and rationality of the proposed bi-level allocation model.

By 2020, there will be 2.36 million natural villages in China, with more than 500 million people living in them. Although China’s 2018 “National Rural Revitalization Strategy” has clarified the importance of the rural environment, the sustainability of living environments in rural settlements is generally ignored. However, current renovation work is implemented as per the same set of standards and is not sufficient to reach the highest satisfaction levels and sustainable development because the optimal renovation indicators are not selected according to local conditions. To address these issues, this work carried out a series of literature reviews and expert interviews to propose indicators applicable to the sustainable renovation of living environments in rural settlements in China. The questionnaire survey was conducted in a typical Chinese village in order to verify the feasibility of this decision model. Furthermore, the Kano model was organically combined with the importance–satisfaction (IS) model from the villagers’ point of view to establish a model analyzing the optimal indicators of the sustainable renovation. The results show that this model determines the priority of elements that need to be improved or maintained, based on which village administrators can make better management decisions and which government managers can invest limited government resources in key sectors, thus creating sustainable living environments in rural settlements and enhancing the satisfaction of villagers. It is suggested that the opinions of village administrators and planners be considered to optimize this model further.

Background: Stroke generates significant health and social burdens. Self-management has potential importance for supporting individuals in coping and continuing to progress after stroke. However, there is a lack of targeted programs to enhance self-management and reduce self-perceived burden (SPB) following stroke.Purpose: To evaluate the effects of a goal-oriented intervention on self-management behaviors and SPB among patients after acute stroke.Methods: This was a randomized controlled trial with a 4-weeks intervention. Participants were randomly allocated to the intervention (n = 48) or control group (n = 48). The intervention and control groups received eight sessions of goal-oriented self-management intervention based on Pender's health promotion model and control care, respectively. Self-management behaviors and SPB were evaluated and compared between the two groups.Results: After the 1-month follow-up, there were significant differences in the total self-management behaviors score and the scores of six of the self-management dimensions, excluding diet management, between the intervention group and the control group (t = −7.891– −2.815; p ≤ 0.006). Compared to the control group, the intervention group showed a significant decrease in the physical burden, emotional burden, and total SPB scores (t = 2.102–2.071; p = 0.015–0.041). The economic burden score was not significantly different between the two groups (t = 1.707; p = 0.091).Conclusion: The goal-oriented intervention based on Pender's health promotion model can effectively improve self-management behaviors and reduce physical and emotional SPB among stroke survivors.

Vibrio cholerae and Vibrio parahaemolyticus are common diarrheal pathogens of great public health concern. A multiplex TaqMan-based real-time PCR assay was developed on the BD MAX platform; this assay can simultaneously detect and differentiate V. cholerae and V. parahaemolyticus directly from human fecal specimens. The assay includes two reactions. One reaction, BDM-VC, targets the gene ompW, the cholera toxin (CT) coding gene ctxA, the O1 serogroup specific gene rfbN, and the O139 serogroup specific gene wbfR of V. cholerae. The other, BDM-VP, targets the gene toxR and the toxin coding genes tdh and trh of V. parahaemolyticus. In addition, each reaction contains a sample process control. When evaluated with spiked stool samples, the detection limit of the BD MAX assay was 195–780 CFU/ml for V. cholerae and 46–184 CFU/ml for V. parahaemolyticus, and the amplification efficiency of all genes was between 95 and 115%. The assay showed 100% analytical specificity, using 63 isolates. The BD MAX assay was evaluated for its performance compared with conventional real-time PCR after automated DNA extraction steps, using 164 retrospective stool samples. The overall percent agreement between the BD MAX assay and real-time PCR was ≥ 98.8%; the positive percent agreement was 85.7% for ompW, 100% for toxR/tdh, and lower (66.7%) for trh because of a false negative. This is the first report to evaluate the usage of the BD MAX open system in detection and differentiation of V. cholerae and V. parahaemolyticus directly from human samples.

Background A link between uric acid (UA) levels and cardiovascular diseases has been previously reported. However, its importance as a risk factor is still controversial. This study sought to determine whether elevated serum uric acid levels are associated with cardiovascular disease (CVD) in middle-aged and elderly Chinese individuals. Methods We conducted a population-based cross-sectional study in Shanghai, with a total of 8510 participants aged ⩾40 years. The CVD included diagnosed coronary heart disease (CHD) and stroke. MetS was defined according to the updated National Cholesterol Education Program Adult Treatment Panel III criteria for Asian Americans. Results Uric acid levels were positively associated with BMI, waist circumference, triglycerides, systolic blood pressure, diastolic blood pressure, glycohemoglobin, fasting plasma glucose, postprandial 2-hour plasma glucose (all P < 0.05), and negatively associated with HDL-cholesterol (P < 0.001). The prevalence of CVD significantly increased with increasing quartiles of UA in those without MetS group (p trend < 0.001), but not necessarily increased in those with MetS. After adjustment for metabolic syndrome and other cardiovascular risk factors, multivariate logistic regression analysis showed that odds ratios (OR) for CHD, stroke, and CVD in those in the fourth quartiles were 2.34 (95 % confidence interval [CI] 1.73 to 3.45), 2.18 (95 % CI 1.86 to 3.28), and 2.16 (95 % CI 1.80 to 3.29), respectively, compared with those in the first quartile of UA. Conclusions Elevated serum uric acid level was associated with CVD, independent of conventional cardiovascular disease risk factors and metabolic syndrome. [ABSTRACT FROM AUTHOR]

Abstract We study the three-body radiative decay $$B_s\rightarrow \phi \bar{K}^0 \gamma $$ Bs→ϕK¯0γ by introducing the $$\phi K$$ ϕK pair distribution amplitudes (DAs) in the perturbative QCD approach. This nonperturbative input, the two-meson DAs, is very important to simplify the calculations. Besides the dominant electromagnetic penguin operator $$O_{7\gamma }$$ O7γ , the subleading contributions from the chromomagnetic penguin operator $$O_{8g}$$ O8g , quark-loop corrections and annihilation type amplitudes are also considered. We find that the branching ratio for the decay $$B_s\rightarrow \phi \bar{K}^0 \gamma $$ Bs→ϕK¯0γ is about $$(9.26^{+1.79+3.12+0.64}_{-1.61-3.86-0.49})\times 10^{-8}$$ (9.26-1.61-3.86-0.49+1.79+3.12+0.64)×10-8 , which is much smaller than that for the decay $$B^0\rightarrow \phi K^0\gamma $$ B0→ϕK0γ . It is mainly because the former decay induced by $$b\rightarrow d\gamma $$ b→dγ with small CKM matrix element is proportional to $$\lambda ^3$$ λ3 . The prediction for the direct CP asymmetry is $$A^{dir}_{CP}(B_s\rightarrow \phi \bar{K}^0 \gamma )=(-4.1^{+0.4+1.7+0.2}_{-0.6-1.2-0.1})\%$$ ACPdir(Bs→ϕK¯0γ)=(-4.1-0.6-1.2-0.1+0.4+1.7+0.2)% , which is well consistent with the result from the U-spin symmetry approach. We also predict the $$B_s \rightarrow \phi \bar{K}^0\gamma $$ Bs→ϕK¯0γ decay spectrum, which exhibits a maximum at the $$\phi K$$ ϕK invariant masss around 1.95 GeV.

Controlling shape-shift and size-growth in nanostructures are important developments in nanoscience but controlling morphology change with an instant on/off function remains challenging. Here the authors demonstrate control over morphology and size transformation of POSS molecules based on living thiol-disulfide exchange reactions.

Abstract We study the B meson decays $$B\rightarrow J/\Psi K_1(1270,1400)$$ B→J/ΨK1(1270,1400) in the pQCD approach beyond the leading order. With the vertex corrections and the NLO Wilson coefficients included, the branching ratios of the considered decays are predicted as $$Br(B^+\rightarrow J/\Psi K_1(1270)^+)=1.76^{+0.65}_{-0.69}\times 10^{-3}, Br(B^+\rightarrow J/\Psi K_1(1400)^+)=6.47^{+2.50}_{-2.34}\times 10^{-4}$$ Br(B+→J/ΨK1(1270)+)=1.76-0.69+0.65×10-3,Br(B+→J/ΨK1(1400)+)=6.47-2.34+2.50×10-4 , and $$Br(B^0\rightarrow J/\Psi K_1(1270)^0)=(1.63^{+0.60}_{-0.64})\times 10^{-3}$$ Br(B0→J/ΨK1(1270)0)=(1.63-0.64+0.60)×10-3 with the mixing angle $$\theta _{K_1}=33^\circ $$ θK1=33∘ , which can agree well with the data or the present experimental upper limit within errors. So we support the opinion that $$\theta _{K_1}\sim 33^\circ $$ θK1∼33∘ is much more favored than $$58^{\circ }$$ 58∘ . Furthermore, we also give the predictions of the polarization fractions, the direct CP violations, the relative phase angles for the considered decays with the mixing angle $$\theta _{K_1}=33^\circ $$ θK1=33∘ and $$58^\circ $$ 58∘ , respectively. The direct CP violations of the two charged decays $$B^+\rightarrow J/\Psi K_1(1270,1400)^+$$ B+→J/ΨK1(1270,1400)+ are very small $$(10^{-4}\sim 10^{-5})$$ (10-4∼10-5) , because the weak phase is very tiny. In order to check the dependence of the results on the nonperturbative input parameters, we also calculate them by using the harmonic-oscillator type wave functions for the $$J/\Psi $$ J/Ψ meson. These results can be tested at the running LHCb and forthcoming Super-B experiments.

Nanostructured La0.6Sr0.4Co0.2Fe0.8O3−δ (LSCF) oxide powder was synthesized by a facile autocombustion process based on a modified glycine-nitrate process (GNP) using cellulose fiber as micro-reactor. As compared with the normal GNP, this novel process allows the combustion to proceed in a much more environmentally friendly and controllable way. The resulted powder is nanocrystallized with particle size of only 15–20 nm as observed by transmission electron microscopy examination. More importantly, because the metal ions could disperse homogenously in cellulose–GN precursor, SrCO3 impurity was effectively suppressed. The concentrations of SrCO3 impurity in LSCF products were determined by carbon dioxide–temperature-programmed desorption technique, which decreased to as low as 1.3 wt% from cellulose–GN process, in contrast to 4.3 wt% from the normal GNP. These features resulted in the attractive improvement of its cathode performance in solid-oxide fuel cells (SOFCs). The interfacial resistances of only ∼0.70 and ∼0.36 Ω·cm2 at 600° and 650°C under air, respectively, were observed, which was about two times better than the LSCF cathode derived from the normal GNP. A peak power density of ∼346 mW/cm2 was achieved at 600°C with cellulose–GN-derived LSCF cathode based on thin-film Sm0.2Ce0.8O1.9 electrolyte SOFC using 3% humidified H2 as the fuel. [ABSTRACT FROM AUTHOR]

This work aimed to characterize a 29-kb blaKPC-2-carrying plasmid, pR31-KPC, from a multidrug resistant strain of Pseudomonas aeruginosa isolated from the sputum of an elderly patient with multiple chronic conditions in China. The backbone of pR31-KPC is closely related to four other blaKPC-2-carrying plasmids, YLH6_p3, p1011-KPC2, p14057A, and pP23-KPC, none of which have been assigned to any of the known incompatibility groups. Two accessory modules, the IS26-blaKPC-2-IS26 unit and IS26-ΔTn6376-IS26 region, separated by a 5.9-kb backbone region, were identified in pR31-KPC, which was also shown to carry the unique resistance marker blaKPC-2. A comparative study of the above five plasmids showed that p1011-KPC2 may be the most complete plasmid of this group to be reported, while pR31-KPC is the smallest plasmid having lost most of its conjugative region. Regions between the iterons and orf207 in the backbone may be hot spots for the acquisition of exogenous resistance entities. The accessory regions of these plasmids have all undergone several biological events when compared with Tn6296. The further transfer of blaKPC-2 in these plasmids may be initiated by either the Tn3 family or IS26-associated transposition or homologous recombination. The data presented here will contribute to a deeper understanding of blaKPC-2 carrying plasmids in Pseudomonas.

The article focuses on a study which examined the synthesis of perovskite-type mixed-conducting oxide of SrSc0.05Co0.95O3-δ (SSC). The synthesis was by suing a combined ethylenediaminetetraacetic acid (EDTA)-citrate complexing method. The SSC oxide was fabricated into dense ceramic membrane for oxygen separation through pressing and sintering process. The study found that the permeation process of SSC was found to be affected by slow oxygen surface exchange kinetics. It was also able to determine the activation energy for oxygen surface exchange and oxygen bulk diffusion.

Abstract Background Among CESA-like gene superfamily, the cellulose synthase-like D (CSLD) genes are most similar to cellulose synthase genes and have been reported to be involved in tip-growing cell and stem development. However, there has been no genome-wide characterization of this gene subfamily in cotton. We thus sought to analyze the evolution and functional characterization of CSLD proteins in cotton based on fully sequenced cotton genomes. Results A total of 23 full-length CSLD proteins were identified in Gossypium raimondii, Gossypium arboreum and Gossypium hirsutum. The phylogenetic tree divided the CSLD proteins into five clades with strong support: CSLD1, CSLD2/3, CSLD4, CSLD5 and CSLD6. The total expression of GhCSLD genes was the highest in androecium & gynoecium (mostly contributed by CSLD1 and CSLD4) compared with other CSL genes. CSLD1 and CSLD4 were only highly expressed in androecium & gynoecium (A&G), and showed tissue-specific expression. The total expression of CSLD2/3, 5 and 6 was highest in the specific tissues. These results suggest that CSLD genes showed the different pattern of expression. Cotton CSLD proteins were subjected to different evolutionary pressures, and the CSLD1 and CSLD4 proteins exhibited episodic and long-term shift positive selection. The predicted three-dimensional structure of GrCSLD1 suggested that GrCSLD1 belongs to glycosyltransferase family 2. The amino acid residues under positive selection in the CSLD1 lineage are positioned in a region adjacent to the class-specific region (CSR), β1-strand and transmembrane helices (TMHs) in the GrCSLD1structure. Conclusion Our results characterized the CSLD proteins by an integrated approach containing phylogeny, transcriptional profiling and 3D modeling. The study added to the understanding about the importance of the CSLD family and provide a useful reference for selecting candidate genes and their associations with the biosynthesis of the cell wall in cotton.

With the increasing number of electric vehicles (EVs), how to make full use of EVs to a peak shaving and valley filling effect on the electrical load, realise the effective interaction between EVs and power grid has become a hot spot of research. However, EV charging is random and uncertain on the characteristics of time and space, in this study, the authors consider the interaction between the EV and the power grid as the foraging behaviour of birds combined with the construction of EV charging station, and build a charge–discharge model based on a particle swarm optimisation algorithm to achieve optimal results through group cooperation. Finally, the feasibility of the scheme is verified by a numerical example.

Brevicoryne brassicae is a problematic pest in cabbage and other field crops. Synthetic pesticides are used to control this pest, but they are injurious for human health and the environment. The present study aimed to purify and identify the active compounds from Citrullus colocynthis leaves with an appraisal of their efficacy against B. brassicae. Separation and purification were performed via different chromatographic techniques. Molecular analysis and chemical structures were recognized by mass spectrum (MS) and nuclear magnetic resonance (NMR), respectively. Moreover, in vitro and in vivo aphicidal activity was assessed using various concentrations, i.e., 6.25, 12.5, 25 and 50 µg/mL at 12, 24, 48 and 72 h exposure. The outcome shows that mass spectrum analyses of the purified compounds suggested the molecular formulae are C30H50O and C29H50O, C29H48O. The compounds were characterized as fernenol and a mixture of spinasterol, 22,23-dihydrospinasterol by 1H-NMR and 13C-NMR spectrum analysis. The toxicity results showed that the mixture of spinasterol and 22,23-dihydrospinasterol showed LC50 values of 32.36, 44.49 and 37.50 µg/mL by contact, residual and greenhouse assay at 72 h exposure, respectively. In contrast, fernenol recorded LC50 values as 47.99, 57.46 and 58.67 µg/mL, respectively. On the other hand, spinasterol, 22,23-dihydrospinasterol showed the highest mortality, i.e., 66.67%, 53.33% and 60% while, 30%, 23.33% and 25% mortality was recorded by fernenol after 72 h at 50 µg/mL by contact, residual and greenhouse assay, respectively. This study suggests that spinasterol, 22,23-dihydrospinasterol are more effective against B. brassicae which may be introduced as an effective and suitable substitute of synthetic chemical pesticides.

Fault diagnosis technology (FDT) is an effective tool to ensure stability and reliable operation in wind turbines. In this paper, a novel fault diagnosis methodology based on a cloud bat algorithm (CBA)-kernel extreme learning machines (KELM) approach for wind turbines is proposed via combination of the multisensor data fusion technique and time-domain analysis. First, the derived method calculates the time-domain indices of raw signals, and the fused time-domain indexes dataset are obtained by the multisensor data fusion. Then, the CBA-based KELM recognition model that can identify fault patterns of a wind turbine gearbox (WTB) is automatically established with the fused dataset. The dataset includes a large number of samples involving 6 fault types under different operational conditions by 5 accelerometers. The effectiveness and feasibility of this proposed method are proved by adopting the datasets originated from the test rig, and it achieves a diagnostic accuracy of 96.25%. Finally, compared with the other peer-to-peer methods, the experimental classification results show that the proposed CBA-KELM technique has the best performances.

Inflammatory bowel disease (IBD) is associated with dysregulation of both innate and adaptive immune response in the intestine. MicroRNA (miR)-155 is frequently expressed and functions in many immune cell types. Besides its function in adaptive immunity, miR-155 is a key regulator of the innate immune response in macrophages, dendritic cells, and even in epithelia cells. Although the roles of miR-155 within T and B lymphocytes in colitis have been reported, its function in innate immune cells has not been thoroughly examined. In this study, the dextran sulfate sodium (DSS)-induced colitis model was established in wild-type (WT) and miR-155−/− mice. Our results showed that miR-155 deficiency in macrophages recapitulated the alleviated colitis feature of miR-155−/− mice and appeared to skew toward the alterative M2 phenotype. Notably, the predominance of M2 in colon can result in dampened intestinal immune cell proliferation and inhibit CD4 T cell polarization toward Th1 and Th17. Moreover, C/EBPβ and SOCS1 were demonstrated as two key functional targets in this process. We also provided evidence for use of miR-155 inhibitor to treat colitis. Collectively, the findings highlight the central role of alternative M2 skewing for miR-155 function in colitis and reveal that macrophages might be a main target for therapeutics.

In recent years, with rising bioengineering, the improving saline-alkali soils of biological measures have become the lasting, stable and effective measures in favour of water and soil conservation and ecological balance. More studies have shown that the biological measures are the most effective and basic measures to improve saline-alkali soils, especially in the inland. Radix astragali is classified to leguminous plant and is a kind of non-toxic and widely used Chinese herbal medicine which is used in cardiac, diuretic, anti- inflammatory, germicide and immunity improvement because of being slightly sweet and tepid. This paper mainly studied the salt tolerance and restraint effects of Radix astragali in saline-alkali soils. In the experiment, different cultivation conditions were applied to investigate the improving saline-alkali soils effect of Radix astragali, which should provide an effective and system scientific technological approach for biological salt control and developing medicinal industry in the saline-alkali soils.

The electricity spot market is now being implemented in China. Demand response, as a kind of flexible resource, is also being studied and explored for the constructed power market. Among the many demand response applications, the virtual power plant (VPP) as an aggregator of distributed energy resources (DERs), receives ever-increasing attention. However, the participation manner and related impacts of the VPP to the electricity spot market are still unknown within the current power market rules. Under this background, obeying the present trading rules of China’s electricity spot market, a two-stage dispatching model with optimized bidding and operating strategy in the day-ahead (DA) and real-time (RT) market for the VPP is proposed. In the designed model, the conditional risk value (CVaR) is adopted to address the risk encountered by the uncertainty of the electricity spot market price. The impact of the user-side over-deviated revenue mechanism (UORM) of the China spot market on the income of the VPP in the DA and RT market is also analyzed. For a full evaluation, different coefficients for the influence of DA and RT risk, UORM, and energy storage system (ESS) are tested to investigate their respective impacts on the revenue of the VPP. The simulation cases prove that the proposed method is helpful for the VPP to optimize DERs’ output in the electricity spot market according to its own risk preference.

The experiments of reactor neutron irradiation which induce dark signal increase in COTS array CCDs are presented. The flux of the reactor neutron beams was about 1.33 × 108 n/cm2s. The three samples were exposed to 1MeV neutron-equivalent fluences of 1 × 1011, 5 × 1011, and 1 × 1012 n/cm2, respectively. The mean dark signal (KD), dark signal non-uniformity (DSNU), and dark signal spikes (hot pixels) versus neutron fluence are investigated. The degradation mechanisms of the dark signal in CCDs are analyzed. The mean dark signal increase due to neutron displacement damage appears to be proportional to displacement damage dose. The dark images from the CCDs irradiated by neutrons are presented to investigate the generation of dark signal spike. The 1D and 2D figures which show the output signal voltage of pixels in dark images irradiated by different neutron beam fluences, are presented to compare the degradation of KD, DSNU, and dark signal spike.

In this work, the positively or negatively charged anatase TiO2 nanoparticles were synthesized via a low temperature precipitation-peptization process (LTPPP) in the presence of poly(ethyleneimine) (PEI) and poly(sodium4- styrenesulfonate) (PSS). X-ray diffraction (XRD) pattern and high-resolution transmission electron microscope (HRTEM) confirmed the anatase crystalline phase. The charges of the prepared TiO2, PEI-TiO2 and PSS-TiO2 nanoparticles were investigated by zeta potentials. The results showed that the zeta potentials of PEI-TiO2 nanoparticles can be tuned from +39.47 mV to +95.46 mV, and that of PSS-TiO2 nanoparticles can be adjusted from -56.63 mV to -119.32 mV. In comparison with TiO2, PSS-TiO2 exhibited dramatic adsorption and degradation of dye molecules, while the PEI modified TiO2 nanoparticles showed lower photocatalytic activity. The photocatalytic performances of these charged nanoparticles were elucidated by the results of UV-vis diffuse reflectance spectra (DRS) and the photoluminescence (PL) spectra, which indicated that the PSS-TiO2 nanoparticles showed a lower recombination rate of electron-hole pairs than TiO2 and PEI-TiO2.

AMP-activated protein kinase (AMPK) plays an important role in mediating energy metabolism and is controlled mainly by two upstream kinases, LKB1 or Ca(2+)/calmodulin-dependent protein kinase kinase-β (CaMKKβ). Previously, we found that baicalin, one of the major flavonoids in a traditional Chinese herb medicine, Scutellaria baicalensis, protects against the development of hepatic steatosis in rats feeding with a high-fat diet by the activation of AMPK, but, the underlying mechanism for AMPK activation is unknown. Here we show that in two LKB1-deficient cells, HeLa and A549 cells, baicalin activates AMPK by α Thr-172 phosphorylation and subsequent phosphorylation of its downstream target, acetyl CoA carboxylase, at Ser-79, to a similar degree as does in HepG2 cells (that express LKB1). Pharmacologic inhibition of CaMKKβ by its selective inhibitor STO-609 markedly inhibits baicalin-induced AMPK activation in both HeLa and HepG2 cells, indicating that CaMKKβ is the responsible AMPK kinase. We also show that treatment of baicalin causes a larger increase in intracellular Ca(2+) concentration ([Ca(2+)](i)), although the maximal level of [Ca(2+)](i) is lower in HepG2 cells compared to HeLa cells. Chelation of intracellular free Ca(2+) by EDTA and EGTA, or depletion of intracellular Ca(2+) stores by the endoplasmic reticulum Ca(2+)-ATPase inhibitor thapsigargin abrogates baicalin-induced activation of AMPK in HeLa cells. Neither cellular ATP nor the production of reactive oxygen species is altered by baicalin. Finally, in HeLa cells, baicalin treatment no longer decreases intracellular lipid accumulation caused by oleic acid after inhibition of CaMKKβ by STO-609. These results demonstrate that a potential Ca(2+)/CaMKKβ dependent pathway is involved in the activation of AMPK by baicalin and suggest that CaMKKβ likely acts as an upstream kinase of AMPK in response to baicalin.