171 results on '"Hideaki Ogata"'
Search Results
52. Streptococcal toxic-shock syndrome due to Streptococcus dysgalactiaesubspecies equisimilis in breast cancer-related lymphedema: a case report
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Syunsuke Magoshi, Miho Yoshida, Fumi Saito, Makoto Sumazaki, Hironori Kaneko, Yorichika Kubota, and Hideaki Ogata
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0301 basic medicine ,medicine.medical_specialty ,Pathology ,Breast Cancer Lymphedema ,030106 microbiology ,lcsh:Medicine ,Case Report ,03 medical and health sciences ,Breast cancer ,Risk Factors ,Intensive care ,Internal medicine ,Streptococcal Infections ,medicine ,Humans ,Blood culture ,Lymphedema ,Disseminated intravascular coagulation ,Aged, 80 and over ,medicine.diagnostic_test ,biology ,Septic shock ,business.industry ,Clindamycin ,lcsh:R ,Streptococcus dysgalactiae subspecies equisimilis ,Streptococcus ,Streptococcal toxic shock syndrome ,Cellulitis ,Penicillin G ,General Medicine ,medicine.disease ,biology.organism_classification ,Shock, Septic ,Anti-Bacterial Agents ,030104 developmental biology ,Arm ,Female ,business ,Streptococcus dysgalactiae - Abstract
Breast cancer-related lymphedema often causes cellulitis and is one of the most common complications after breast cancer surgery. Streptococci are the major pathogens underlying such cellulitis. Among the streptococci, the importance of the Lancefield groups C and G is underappreciated; most cases involve Streptococcus dysgalactiae subspecies equisimilis. Despite having a relatively weak toxicity compared with group A streptococci, Streptococcus dysgalactiae subspecies equisimilis is associated with a mortality rate that is as high as that of group A streptococci in cases of invasive infection because Streptococcus dysgalactiae subspecies equisimilis mainly affects elderly individuals who already have various comorbidities. An 83-year-old Japanese woman with breast cancer-related lymphedema in her left upper limb was referred to our hospital with high fever and acute pain with erythema in her left arm. She showed septic shock with disseminated intravascular coagulation. Blood culture showed positive results for Streptococcus dysgalactiae subspecies equisimilis, confirming a diagnosis of streptococcal toxic-shock syndrome. She survived after successful intensive care. To the best of our knowledge, this case represents the first report of Streptococcus dysgalactiae subspecies equisimilis-induced streptococcal toxic-shock syndrome in a patient with breast cancer-related lymphedema. Breast cancer-related lymphedema is a common problem, and we must pay attention to invasive streptococcal soft tissue infections, particularly in elderly patients with chronic disease.
- Published
- 2017
53. Structural Studies of Matrix Metalloproteinase by X-Ray Diffraction
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Elena, Decaneto, Wolfgang, Lubitz, and Hideaki, Ogata
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Models, Molecular ,Protein Conformation ,Catalytic Domain ,Recombinant Fusion Proteins ,Escherichia coli ,Matrix Metalloproteinase 14 ,Crystallography, X-Ray ,Protein Engineering ,Protein Refolding - Abstract
Matrix Metalloproteinases (MMPs) are a family of proteolytic enzymes whose endopeptidase activity is dependent on the presence of specific metal ions. MT1-MMP (or MMP-14), which has been implicated in tumor progression and cellular invasion, contains a membrane-spanning region located C-terminal to a hemopexin-like domain and an N-terminal catalytic domain. We recombinantly expressed the catalytic domain of human MT1-MMP in E. coli and purified it from inclusion bodies using a refolding protocol that yielded significant quantities of active protein. Crystals of MT1-MMP were obtained using the vapour diffusion method. Here, we describe the protocols used for crystallization and the data analysis together with the resulting diffraction pattern.
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- 2017
54. Solvent water interactions within the active site of the membrane type I matrix metalloproteinase
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Yuri Kutin, Nicholas Cox, Tatiana Vasilevskaya, Irit Sagi, Hideaki Ogata, Wolfgang Lubitz, Walter Thiel, Martina Havenith, Elena Decaneto, and Moran Grossman
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0301 basic medicine ,Models, Molecular ,Coordination sphere ,Stereochemistry ,Protein Conformation ,General Physics and Astronomy ,Protonation ,Peptide ,Matrix Metalloproteinase Inhibitors ,010402 general chemistry ,01 natural sciences ,Catalysis ,03 medical and health sciences ,Protein structure ,Catalytic Domain ,Matrix Metalloproteinase 14 ,Physical and Theoretical Chemistry ,chemistry.chemical_classification ,Binding Sites ,biology ,Chemistry ,Active site ,Rate-determining step ,0104 chemical sciences ,030104 developmental biology ,Catalytic cycle ,biology.protein ,Solvents ,Amine gas treating - Abstract
Matrix metalloproteinases (MMP) are an important family of proteases which catalyze the degradation of extracellular matrix components. While the mechanism of peptide cleavage is well established, the process of enzyme regeneration, which represents the rate limiting step of the catalytic cycle, remains unresolved. This step involves the loss of the newly formed N-terminus (amine) and C-terminus (carboxylate) protein fragments from the site of catalysis coupled with the inclusion of one or more solvent waters. Here we report a novel crystal structure of membrane type I MMP (MT1-MMP or MMP-14), which includes a small peptide bound at the catalytic Zn site via its C-terminus. This structure models the initial product state formed immediately after peptide cleavage but before the final proton transfer to the bound amine; the amine is not present in our system and as such proton transfer cannot occur. Modeling of the protein, including earlier structural data of Bertini and coworkers [I. Bertini, et al., Angew. Chem., Int. Ed., 2006, 45, 7952-7955], suggests that the C-terminus of the peptide is positioned to form an H-bond network to the amine site, which is mediated by a single oxygen of the functionally important Glu240 residue, facilitating efficient proton transfer. Additional quantum chemical calculations complemented with magneto-optical and magnetic resonance spectroscopies clarify the role of two additional, non-catalytic first coordination sphere waters identified in the crystal structure. One of these auxiliary waters acts to stabilize key intermediates of the reaction, while the second is proposed to facilitate C-fragment release, triggered by protonation of the amine. Together these results complete the enzymatic cycle of MMPs and provide new design criteria for inhibitors with improved efficacy.
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- 2017
- Full Text
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55. Structural Studies of Matrix Metalloproteinase by X-Ray Diffraction
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Hideaki Ogata, Wolfgang Lubitz, and Elena Decaneto
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0301 basic medicine ,Metalloproteinase ,Chemistry ,Proteolytic enzymes ,Matrix metalloproteinase ,Inclusion bodies ,law.invention ,03 medical and health sciences ,030104 developmental biology ,Endopeptidase activity ,law ,Tumor progression ,X-ray crystallography ,Biophysics ,Crystallization - Abstract
Matrix Metalloproteinases (MMPs) are a family of proteolytic enzymes whose endopeptidase activity is dependent on the presence of specific metal ions. MT1-MMP (or MMP-14), which has been implicated in tumor progression and cellular invasion, contains a membrane-spanning region located C-terminal to a hemopexin-like domain and an N-terminal catalytic domain. We recombinantly expressed the catalytic domain of human MT1-MMP in E. coli and purified it from inclusion bodies using a refolding protocol that yielded significant quantities of active protein. Crystals of MT1-MMP were obtained using the vapour diffusion method. Here, we describe the protocols used for crystallization and the data analysis together with the resulting diffraction pattern.
- Published
- 2017
56. Crystallization and preliminary X-ray crystallographic analysis of the catalytic domain of membrane type 1 matrix metalloproteinase
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Moran Grossman, Markus Knipp, Elena Decaneto, Wolfgang Lubitz, Irit Sagi, Hideaki Ogata, and Martina Havenith
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Protein Conformation ,Chemistry ,Resolution (electron density) ,Biophysics ,Matrix metalloproteinase ,Crystallography, X-Ray ,Condensed Matter Physics ,Biochemistry ,law.invention ,Crystal ,Solvent ,Crystallography ,Membrane ,Crystallization Communications ,Structural Biology ,law ,Catalytic Domain ,PEG ratio ,Genetics ,Humans ,Molecule ,Electrophoresis, Polyacrylamide Gel ,Matrix Metalloproteinase 1 ,Crystallization - Abstract
Membrane type 1 matrix metalloproteinase (MT1-MMP) belongs to the large family of zinc-dependent endopeptidases termed MMPs that are located in the extracellular matrix. MT1-MMP was crystallized at 277 K using the vapour-diffusion method with PEG as a precipitating agent. Data sets for MT1-MMP were collected to 2.24 Å resolution at 100 K. The crystals belonged to space group P43212, with unit-cell parameters a = 62.99, c = 122.60 Å. The crystal contained one molecule per asymmetric unit, with a Matthews coefficient (V M) of 2.90 Å3 Da−1; the solvent content is estimated to be 57.6%.
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- 2014
57. Nitric oxide heme interactions in nitrophorin 7 investigated by nuclear inelastic scattering
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S. Rackwitz, Isabelle Faus, Hendrik Auerbach, Markus Knipp, A. I. Chumakov, Hideaki Ogata, F. A. Walker, Juliusz A. Wolny, and Volker Schünemann
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Nuclear and High Energy Physics ,biology ,Inorganic chemistry ,Inelastic scattering ,Condensed Matter Physics ,biology.organism_classification ,Molecular mechanics ,Atomic and Molecular Physics, and Optics ,Nitric oxide ,chemistry.chemical_compound ,chemistry ,Nitrophorin ,medicine ,Biophysics ,Ferric ,Density functional theory ,Physical and Theoretical Chemistry ,Rhodnius prolixus ,Heme ,medicine.drug - Abstract
Nitrophorins (NPs) occur in the blood-sucking insect Rhodnius prolixus. These proteins use ferric heme to store nitric oxide (NO) in the salivary glands of the insects and transport it to the victim’s tissues, resulting in vasodilation and reduced blood coagulation. In this work we present a nuclear inelastic scattering (NIS) study in order to characterize the iron-NO interaction in the isoform nitrophorin 7 (NP7). The NIS data obtained for NP7 complexed with NO show a strong band at ∼589 cm−1 which is due to modes with significant Fe-NO stretching and bending character. Another conspicuous feature is a significant peak at ∼280 cm−1 in the region where the heme modes occur. Based on a hybrid calculation method, which uses density functional theory and molecular mechanics, the band at ∼280 cm−1 is assigned to heme modes with substantial doming character.
- Published
- 2013
58. A cryogenic receiver for EPR
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Hideaki Ogata, R. Narkowicz, Eduard J. Reijerse, and Dieter Suter
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Nuclear and High Energy Physics ,Noise temperature ,Materials science ,business.industry ,Noise spectral density ,Biophysics ,Analytical chemistry ,Y-factor ,Condensed Matter Physics ,Biochemistry ,law.invention ,Signal-to-noise ratio ,law ,Optoelectronics ,Resistor ,business ,Sensitivity (electronics) ,Noise (radio) ,Microwave - Abstract
Cryogenic probes have significantly increased the sensitivity of NMR. Here, we present a compact EPR receiver design capable of cryogenic operation. Compared to room temperature operation, it reduces the noise by a factor of ≈2.5. We discuss in detail the design and analyze the resulting noise performance. At low microwave power, the input noise density closely follows the emission of a cooled 50 Ω resistor over the whole measurement range from 20 K up to room temperature. To minimize the influence of the microwave source noise, we use high microwave efficiency (≈1.1–1.7 mT W −1/2 ) planar microresonators. Their efficient conversion of microwave power to magnetic field permits EPR measurements with very low power levels, typically ranging from a few μW down to fractions of nW.
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- 2013
59. A Case of Dermatomyositis Along with Esophageal Cancer and Screening of Serum Transcriptional Intermediary Factor 1 Gamma Antibodies in Various Cancer Patients.
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Makoto Sumazaki, Kaichi Kaneko, Masaaki Ito, Yoko Oshima, Fumi Saito, Hideaki Ogata, Kazutoshi Shibuya, and Hideaki Shimada
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EARLY detection of cancer ,CANCER patients ,DERMATOMYOSITIS ,MUSCLE weakness ,MYALGIA ,ESOPHAGEAL cancer ,CREATINE kinase - Abstract
Objective: Unknown ethiology Background: Dermatomyositis (DM) is occasionally associated with malignancy, which is so-called cancer-associated myositis. The cancer screening in patients with dermatomyositis is an important clinical issue. That is because malignant disease underlying dermatomyositis is potentially life-threatening. Transcriptional intermediary factor 1g (TIF1g) antibodies (anti-TIF1g Abs) are one of the myositis-specific autoantibodies, which are investigated as potential predictors of malignancy in patients with dermatomyositis. However, the etiology of anti-TIF1g Abs generations in various cancer patients is not known. Case Report: A 70-year-old male patient was admitted for muscle pain and weakness in both legs. Erythematous on the face, eruption, and a V sign were also observed. Laboratory tests showed the elevation of creatine kinase, myoglobin, and aldolase. He was diagnosed as dermatomyositis. Cancer screening was performed, and esophageal cancer was detected in the lower esophagus. Despite the symptoms of dermatomyositis were improved with steroid, methotrexate, and radical esophagectomy, he died with esophageal cancer 3 years after the onset of dermatomyositis. TIF1g is frequently overexpressed in cancer tissues. Therefore, some cancer patients without dermatomyositis could be positive for anti-TIF1g Abs. We retrospectively analyzed anti-TIF1g Abs in cancer patients (n=131). However, the screening of anti-TIF1g Abs in cancer patients without dermatomyositis (n=130) showed there were no seropositive patients. Only this cancer-associated myositis patient was positive for anti- TIF1g Abs. Conclusions: Our result suggested the generation of anti-TIF1g Abs is specific for cancer associated myositis, not for tumorigenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
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60. Expression, Purification, and Solid-State NMR Characterization of the Membrane Binding Heme Protein Nitrophorin 7 in Two Electronic Spin States
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Hideaki Ogata, Fei Yang, Victor Pacheco, Henrike Heise, Alexander Medvedev, Sabu Varghese, Markus Knipp, and Kathrin Wrede
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Hemeproteins ,Gene isoform ,Hemeprotein ,biology ,Crystallography, X-Ray ,Resonance (chemistry) ,biology.organism_classification ,Biochemistry ,Heme-Binding Proteins ,chemistry.chemical_compound ,Crystallography ,Solid-state nuclear magnetic resonance ,chemistry ,Rhodnius ,Nitrophorin ,Animals ,Insect Proteins ,Imidazole ,Membrane binding ,Protein Multimerization ,Salivary Proteins and Peptides ,Carrier Proteins ,Rhodnius prolixus ,Nuclear Magnetic Resonance, Biomolecular - Abstract
The nitrophorins (NPs) comprise a group of NO transporting ferriheme b proteins found in the saliva of the blood sucking insect Rhodnius prolixus . In contrast to other nitrophorins (NP1-4), the recently identified membrane binding isoform NP7 tends to form oligomers and precipitates at higher concentrations in solution. Hence, solid-state NMR (ssNMR) was employed as an alternative method to gain structural insights on the precipitated protein. We report the expression and purification of (13)C,(15)N isotopically labeled protein together with the first ssNMR characterization of NP7. Because the size of NP7 (21 kDa) still provides a challenge for ssNMR, the samples were reverse labeled with Lys and Val to reduce the number of crosspeaks in two-dimensional spectra. The two electronic spin states with S = 1/2 and S = 0 at the ferriheme iron were generated by the complexation with imidazole and NO, respectively. ssNMR spectra of both forms are well resolved, which allows for sequential resonance assignments of 22 residues. Importantly, the ssNMR spectra demonstrate that aggregation does not affect the protein fold. Comparison of the spectra of the two electronic spin states allows the determination of paramagnetically shifted cross peaks due to pseudocontact shifts, which assists the assignment of residues close to the heme center.
- Published
- 2013
61. Male Breast Clear Cell Hidradenoma ^|^mdash;A Case Report and Literature Review^|^mdash;
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Fumi Saito, Shinsaku Kanazawa, Hideaki Ogata, Aki Mitsuda, Yorichika Kubota, and Hironori Kaneko
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Clear Cell Hidradenoma ,medicine.medical_specialty ,business.industry ,medicine ,Male breast ,business ,Dermatology - Published
- 2013
62. Contrast-enhanced ultrasound findings with Sonazoid for evaluation of neoadjuvant chemotherapy for breast cancer
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Shinsaku Kanazawa, Kenichi Maruyama, Tetsuo Nemoto, Yorichika Kubota, Kazutoshi Shibuya, Tsuneyoshi Yakuwa, Yukio Mitsuzuka, Fumi Saito, Hironori Kaneko, and Hideaki Ogata
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Oncology ,medicine.medical_specialty ,Chemotherapy ,medicine.diagnostic_test ,business.industry ,medicine.medical_treatment ,medicine.disease ,Breast cancer ,Internal medicine ,medicine ,Radiology, Nuclear Medicine and imaging ,Radiology ,business ,Breast ultrasound ,Contrast-enhanced ultrasound - Published
- 2013
63. Detektion von Fe-CN- und Fe-CO-Schwingungen im aktiven Zentrum der [NiFe]-Hydrogenase durch inelastische kernresonante Streuung
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Brian C. Manor, Deborah Byrne, Wolfgang Lubitz, Michael W. W. Adams, E. Ercan Alp, Francis E. Jenney, Jiyong Zhao, Saeed Kamali, Yoshitaka Yoda, Stephen P. Cramer, Hongxin Wang, Violaine Bonnefoy, Thomas B. Rauchfuss, Devrani Mitra, and Hideaki Ogata
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Chemistry ,General Medicine - Published
- 2012
64. Observation of the FeCN and FeCO Vibrations in the Active Site of [NiFe] Hydrogenase by Nuclear Resonance Vibrational Spectroscopy
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Deborah Byrne, Yoshitaka Yoda, Michael W. W. Adams, Francis E. Jenney, Saeed Kamali, Violaine Bonnefoy, Hideaki Ogata, Devrani Mitra, Stephen P. Cramer, Hongxin Wang, Thomas B. Rauchfuss, Jiyong Zhao, Brian C. Manor, Wolfgang Lubitz, and E. E. Alp
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Models, Molecular ,Hydrogenase ,Analytical chemistry ,Infrared spectroscopy ,Inelastic scattering ,010402 general chemistry ,Vibration ,01 natural sciences ,Article ,Catalysis ,Normal mode ,Catalytic Domain ,Spectroscopy, Fourier Transform Infrared ,Cluster (physics) ,Nuclear resonance vibrational spectroscopy ,Range (particle radiation) ,biology ,010405 organic chemistry ,Chemistry ,Electron Spin Resonance Spectroscopy ,Active site ,General Chemistry ,0104 chemical sciences ,Crystallography ,biology.protein ,Oxidation-Reduction ,Iron Compounds - Abstract
Nuclear inelastic scattering of (57)Fe labeled [NiFe] hydrogenase is shown to give information on different states of the enzyme. It was thus possible to detect and assign Fe-CO and Fe-CN bending and stretching vibrations of the active site outside the spectral range of the Fe-S cluster normal modes.
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- 2012
65. Structure and function of [NiFe] hydrogenases
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Yoshiki Higuchi, Hideaki Ogata, and Wolfgang Lubitz
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0301 basic medicine ,Hydrogenase ,Hydrogen ,chemistry.chemical_element ,Hydrogenase mimic ,010402 general chemistry ,Photochemistry ,01 natural sciences ,Biochemistry ,Heterolysis ,Catalysis ,03 medical and health sciences ,Structure-Activity Relationship ,Bacterial Proteins ,Catalytic Domain ,Molecule ,Molecular Biology ,biology ,Bacteria ,Ligand ,Active site ,General Medicine ,0104 chemical sciences ,030104 developmental biology ,chemistry ,biology.protein - Abstract
Hydrogenases catalyze the reversible conversion of molecular hydrogen to protons and electrons via a heterolytic splitting mechanism. The active sites of [NiFe] hydrogenases comprise a dinuclear Ni-Fe center carrying CO and CN- ligands. The catalytic activity of the standard (O2-sensitive) [NiFe] hydrogenases vanishes under aerobic conditions. The O2-tolerant [NiFe] hydrogenases can sustain H2 oxidation activity under atmospheric conditions. These hydrogenases have very similar active site structures that change the ligand sphere during the activation/catalytic process. An important structural difference between these hydrogenases has been found for the proximal iron-sulphur cluster located in the vicinity of the active site. This unprecedented [4Fe-3S]-6Cys cluster can supply two electrons, which lead to rapid recovery of the O2 inactivation, to the [NiFe] active site.
- Published
- 2016
66. Heterogeneous Kinetics of the Carbon Monoxide Association and Dissociation Reaction to Nitrophorin 4 and 7 Coincide with Structural Heterogeneity of the Gate-Loop
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Chunmao He, Markus Knipp, Hideaki Ogata, Cristiano Viappiani, Stefano Bruno, and Stefania Abbruzzetti
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Hemeproteins ,Kinetics ,Spectrum Analysis, Raman ,Photochemistry ,Kinetic energy ,Biochemistry ,Catalysis ,Dissociation (chemistry) ,chemistry.chemical_compound ,symbols.namesake ,Colloid and Surface Chemistry ,Spectroscopy, Fourier Transform Infrared ,Animals ,Molecule ,Salivary Proteins and Peptides ,Spectroscopy ,Carbon Monoxide ,Photolysis ,General Chemistry ,Nanosecond ,chemistry ,Rhodnius ,symbols ,Spectrophotometry, Ultraviolet ,Raman spectroscopy ,Carbon monoxide - Abstract
NO is an important signaling molecule in human tissue. However, the mechanisms by which this molecule is controlled and directed are currently little understood. Nitrophorins (NPs) comprise a group of ferriheme proteins originating from blood-sucking insects that are tailored to protect and deliver NO via coordination to and release from the heme iron. Therefore, the kinetics of the association and dissociation reactions were studied in this work using the ferroheme-CO complexes of NP4, NP4(D30N), and NP7 as isoelectronic models for the ferriheme-NO complexes. The kinetic measurements performed by nanosecond laser-flash-photolysis and stopped-flow are accompanied by resonance Raman and FT-IR spectroscopy to characterize the carbonyl species. Careful analysis of the CO rebinding kinetics reveals that in NP4 and, to a larger extent, NP7 internal gas binding cavities are located, which temporarily trap photodissociated ligands. Moreover, changes in the free energy barriers throughout the rebinding and release pathway upon increase of the pH are surprisingly small in case of NP4. Also in case of NP4, a heterogeneous kinetic trace is obtained at pH 7.5, which corresponds to the presence of two carbonyl species in the heme cavity that are seen in vibrational spectroscopy and that are due to the change of the distal heme pocket polarity. Quantification of the two species from FT-IR spectra allowed the fitting of the kinetic traces as two processes, corresponding to the previously reported open and closed conformation of the A-B and G-H loops. With the use of the A-B loop mutant NP4(D30N), it was confirmed that the kinetic heterogeneity is controlled by pH through the disruption of the H-bond between the Asp30 side chain and the Leu130 backbone carbonyl. Overall, this first study on the slow phase of the dynamics of diatomic gas molecule interaction with NPs comprises an important experimental contribution for the understanding of the dynamics involved in the binding/release processes of NO/CO in NPs.
- Published
- 2012
67. Guanidin-Ferrohäm-Koordination in der Proteinmutante Nitrophorin 4(L130R)
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Chunmao He, Hideaki Ogata, Markus Knipp, and Martin Fuchs
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Chemistry ,General Medicine - Published
- 2012
68. Guanidine-Ferroheme Coordination in the Mutant Protein Nitrophorin 4(L130R)
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Martin Fuchs, Hideaki Ogata, Chunmao He, and Markus Knipp
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Hemeproteins ,Chemistry ,Nitrophorin 4 ,Water ,Heme ,General Chemistry ,Catalysis ,chemistry.chemical_compound ,Biochemistry ,Mutant protein ,Nitrophorin ,Organometallic Compounds ,Mutant Proteins ,Salivary Proteins and Peptides ,Guanidine ,Group 2 organometallic chemistry - Published
- 2012
69. Squamous Cell Carcinoma of the Breast in a Patient with HIV : A Case Report
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Fumi Saito, Shunsuke Magoshi, Yorichika Kubota, Hideaki Ogata, Tetsuo Nemoto, Chikako Hasegawa, Hironori Kaneko, and Shinsaku Kanazawa
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Oncology ,medicine.medical_specialty ,Breast cancer ,business.industry ,Internal medicine ,medicine ,Human immunodeficiency virus (HIV) ,Basal cell ,medicine.disease ,business ,medicine.disease_cause - Published
- 2012
70. Crystallization and preliminary X-ray crystallographic analysis of the membrane-binding haemprotein nitrophorin 7 fromRhodnius prolixus
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Markus Knipp and Hideaki Ogata
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Hemeproteins ,Models, Molecular ,Rhodnius ,Biophysics ,Crystallography, X-Ray ,Biochemistry ,law.invention ,Crystal ,Structural Biology ,law ,Mutant protein ,Nitrophorin ,Genetics ,Animals ,Salivary Proteins and Peptides ,Crystallization ,Rhodnius prolixus ,biology ,Resolution (electron density) ,Condensed Matter Physics ,biology.organism_classification ,Protein Structure, Tertiary ,Crystallography ,Crystallization Communications ,Nitric oxide transport - Abstract
Nitrophorins (nitric oxide transport proteins) are haemproteins originating from the blood-feeding insect Rhodnius prolixus. They consist of an eight-stranded β-barrel, which classifies them into the lipocalin family. Nitrophorin 7 (NP7) and the E27V mutant protein NP7(E27V) were crystallized at 277 K using the vapour-diffusion method with PEG as the precipitating agent. Data sets for wild-type NP7 and NP7(E27V) were collected to 1.80 Å resolution from single crystals at 100 K using synchrotron radiation. The crystals belonged to space group P2(1), with unit-cell parameters a = 38, b = 67, c = 39 Å, β = 117°. The crystal contained one molecule per asymmetric unit, with a Matthews coefficient (V(M)) of 2.11 Å(3) Da(-1); the solvent content was estimated to be 41.8%.
- Published
- 2011
71. A Tyrosyl−Dimanganese Coupled Spin System is the Native Metalloradical Cofactor of the R2F Subunit of the Ribonucleotide Reductase of Corynebacterium ammoniagenes
- Author
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Patrick Stolle, Wolfgang Lubitz, Georg Auling, Nicholas Cox, Hideaki Ogata, and Edward J. Reijerse
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Models, Molecular ,Stereochemistry ,Protein subunit ,Dimer ,Coenzymes ,chemistry.chemical_element ,Manganese ,Corynebacterium ,Crystallography, X-Ray ,Photochemistry ,Biochemistry ,Protein Structure, Secondary ,Catalysis ,Cofactor ,Residue (chemistry) ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,Oxidoreductase ,Freezing ,Ribonucleotide Reductases ,Tyrosine ,chemistry.chemical_classification ,biology ,Electron Spin Resonance Spectroscopy ,Temperature ,General Chemistry ,Protein Subunits ,Ribonucleotide reductase ,chemistry ,biology.protein ,Dimerization - Abstract
The X-ray crystallographic structure of the native R2F subunit of the ribonucleotide reductase (RNR) of Corynebacterium ammoniagenes ATCC 6872 is reported, with a resolution of 1.36 A. The metal site contains an oxo/hydroxo-bridged manganese dimer, located near a tyrosine residue (Y115). The coordination of the manganese dimer and its distance to a nearby tyrosine residue resemble the di-iron metalloradical cofactor of class I RNR from Escherichia coli . Multifrequency EPR measurements of the highly active C. ammoniagenes R2F subunit show that the metal site contains a ferromagnetically exchange-coupled Mn(III)Mn(III) dimer weakly coupled to a tyrosyl radical. A mechanism for the metalloradical cofactor (Mn(III)Mn(III)Y(*)) generation is proposed. H(2)O(2) (HO(2)(-)) instead of O(2) is hypothesized as physiological oxidant for the Mn dimer which in turn oxidizes the tyrosine Y115. Changes in the ligand sphere of both manganese ions during metalloradical generation direct the complex formation of this cofactor, disfavoring alternate reaction pathways such as H(2)O(2) dismutation, as observed for manganese catalase, a structural analogue of the R2F metal site. The presented results demonstrate the importance of manganese for radical formation in this RNR and confirm the assignment of this enzyme to class Ib.
- Published
- 2010
72. Observation of Condensation Dynamics on Micro-structures by Environmental SEM
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Akira Yamada, Ryosuke Ikeda, Hideaki Ogata, Takanobu Fukunaga, Koji Takahashi, Daniel Orejon, Yasuyuki Takata, and Minemasa Omura
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Materials science ,Chemical engineering ,Condensation ,Wetting ,Environmental scanning electron microscope ,Micro structure - Published
- 2018
73. A Case of Granulomatous Lobular Mastitis Successfully Treated with Corticosteroid
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Yoko Nagai, Fumi Saito, Shunsuke Magoshi, Shinsaku Kanazawa, Hideaki Ogata, Yorichika Kubota, Aki Mitsuda, and Hironori Kaneko
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Pathology ,medicine.medical_specialty ,Granulomatous lobular mastitis ,business.industry ,medicine.drug_class ,medicine ,Corticosteroid ,Granulomatous mastitis ,medicine.disease ,business - Published
- 2010
74. Use of Frame Retractors for Excisional Biopsy of the Breast
- Author
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Tatsuo Teramoto, Hideaki Ogata, Youkou Oshima, Shinsaku Kanazawa, Kazutaka Kimura, Fumi Saito, and Shunsuke Magoshi
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medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Frame (networking) ,Single surgeon ,Surgery ,Aspiration cytology ,Lesion ,Blood loss ,Needle biopsy ,Biopsy ,medicine ,Local anesthesia ,medicine.symptom ,business - Abstract
The use of frame retractors for excisional biopsies of the breast is straightforward and can be performed by a single surgeon without assistance. In addition, since a surgical field is established around the lesion, it is possible to obtain a visual field large enough to permit highly reliable excision of the lesion. We discuss our experience with frame retractors in such surgical procedures. In ten patients with breast lesions that could not be conclusively diagnosed by aspiration cytology and needle biopsy, frame retractors were used to perform surgical biopsies under local anesthesia. Blood loss, duration of surgery, analgesic usage, and complications were assessed. In all cases, the amount of blood loss was low ; the mean duration from the start of anesthesia to wound closure was 41 minutes. In summary, our results show that frame retractors are extremely useful for excisional biopsies of the breast.
- Published
- 2008
75. BETA.-cell Function is a Major Contributor to Oral Glucose Disposition in Obese Japanese Students
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Keizo Anzai, Takashi Asano, Hideaki Ogata, Yuhko Akehi, Ryoko Yoshida, Masao Ogimoto, Kazuhiko Kokawa, Junko Ono, Kazuo Tamura, Kiyomi Hidehira, and Masahiro Kikuchi
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Adult ,Blood Glucose ,Male ,medicine.medical_specialty ,Adolescent ,Endocrinology, Diabetes and Metabolism ,Administration, Oral ,Type 2 diabetes ,Carbohydrate metabolism ,Impaired glucose tolerance ,Endocrinology ,Insulin resistance ,Japan ,Insulin-Secreting Cells ,Internal medicine ,Glucose Intolerance ,medicine ,Humans ,Insulin ,Obesity ,Triglycerides ,Glucose tolerance test ,medicine.diagnostic_test ,business.industry ,Glucose Tolerance Test ,medicine.disease ,Impaired fasting glucose ,Cholesterol ,Cross-Sectional Studies ,Glucose ,Area Under Curve ,Female ,Insulin Resistance ,business ,Homeostasis - Abstract
Determinants of glucose intolerance were studied in 163 obese Japanese young adults, 18 to 21 years old (43 females,120 males), who underwent 75-g oral glucose tolerance testing. Type 2 diabetes was newly diagnosed in 2.9% (n = 4); impaired fasting glucose (IFG) in 5.1% (n = 7); and impaired glucose tolerance (IGT) in 10.9% (n = 15). A homeostasis model assessment of insulin resistance (HOMA-IR) was used to estimate insulin sensitivity; beta-cell function during the first 30 min of the test was measured and defined as the insulinogenic index. This index was adjusted for insulin sensitivity, since this affects both beta-cell function and glucose disposition (disposition index). The relationship between insulinogenic index and 1/HOMA-IR was not hyperbolic. However, the disposition index (DI) was useful for the estimation of beta-cell function with the correct confirmation about it validity using beta-cell function index (BI). The association between insulin sensitivity and beta-cell function to glucose disposal, as measured by the area under the glucose curve (AUCg), was examined in all subjects. Insulin sensitivity was significantly related to AUCg (log HOMA-IR; R (2) = 0.142, p
- Published
- 2007
76. Liver Metastasis of a Triple-Negative Breast Cancer and Complete Remission for 5 Years After Treatment With Combined Bevacizumab/Paclitaxel/Carboplatin: Case Report and Review of the Literature
- Author
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Kazuhiko Natori, Shinsaku Kanazawa, Yorichika Kubota, Yoshihiro Kikuchi, Tadatoshi Osaku, Nobuyuki Shiraga, Masahiro Kobayashi, Hideaki Ogata, Fumi Saito, Shunsuke Magoshi, Hironori Kaneko, and Yoshie Murakami
- Subjects
Oncology ,Adult ,medicine.medical_specialty ,Bevacizumab ,Paclitaxel ,medicine.medical_treatment ,Triple Negative Breast Neoplasms ,Metastasis ,Carboplatin ,chemistry.chemical_compound ,Germline mutation ,Breast cancer ,Pregnancy ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Genetic Predisposition to Disease ,Clinical Case Report ,Triple-negative breast cancer ,Germ-Line Mutation ,BRCA2 Protein ,business.industry ,Liver Neoplasms ,Remission Induction ,Pregnancy Outcome ,General Medicine ,medicine.disease ,Regimen ,chemistry ,Female ,business ,Pregnancy Complications, Neoplastic ,Mastectomy ,medicine.drug ,Research Article - Abstract
Triple-negative breast cancer (TNBC) is aggressive, with high risk of visceral metastasis and death. A substantial proportion of patients with TNBC is associated with BRCA mutations, implying that these tumors are sensitive to DNA-damaging agents. We report successful treatment of a metastatic TNBC in a woman with a BRCA2 germline mutation using combined bevacizumab/paclitaxel/carboplatin (BPC) therapy. The patient was pregnant and had liver metastases, and a complete clinical response was sustained for approximately 5 years. Mastectomy was performed during the 29th week of pregnancy, and the baby was later delivered by caesarean section. Subsequently, multiple metastases in both liver lobes were detected using computed tomography and magnetic resonance imaging and the patient was treated with a BPC regimen, which led to complete disappearance of metastatic lesions in the liver. No additional treatment was provided, and after 5 years the patient consented to direct sequencing of BRCA2 and a 6781delG mutation was identified. At the most recent (5-year) follow-up, the patient was alive with good quality of life and no evidence of metastases. This finding suggests that BPC therapy might be considered a good therapeutic option for the treatment of metastatic TNBC in a woman with a BRCA2 germline mutation.
- Published
- 2015
77. A strenuous experimental journey searching for spectroscopic evidence of a bridging nickel-iron-hydride in [NiFe] hydrogenase
- Author
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Yoshihito Tanaka, Hongxin Wang, Hideaki Ogata, Yoshitaka Yoda, and Wolfgang Lubitz
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Nuclear and High Energy Physics ,Bridging (networking) ,Hydrogenase ,Magnetic Resonance Spectroscopy ,chemistry.chemical_element ,Synchrotron radiation ,010402 general chemistry ,01 natural sciences ,nuclear resonance vibrational spectroscopy ,Computer Simulation ,Nuclear resonance vibrational spectroscopy ,Instrumentation ,Radiation ,Iron hydride ,Binding Sites ,[NiFe] hydrogenase ,010405 organic chemistry ,Hydride ,Hydrogen Bonding ,Feature Articles ,0104 chemical sciences ,Enzyme Activation ,Crystallography ,Nickel ,chemistry ,Models, Chemical ,Physical chemistry ,Density functional theory ,Ni–R ,iron hydride ,ultra-weak signal ,Ni–H–Fe wag mode ,NRVS ,Hydrogen ,Protein Binding - Abstract
Direct spectroscopic evidence for a hydride bridge in the Ni–R form of [NiFe] hydrogenase has been obtained using iron-specific nuclear resonance vibrational spectroscopy. This article focuses on the long and strenuous experimental journey to search for and identify this first spectroscopic evidence for a hydride in Ni–R., Direct spectroscopic evidence for a hydride bridge in the Ni–R form of [NiFe] hydrogenase has been obtained using iron-specific nuclear resonance vibrational spectroscopy (NRVS). The Ni–H–Fe wag mode at 675 cm−1 is the first spectroscopic evidence for a bridging hydride in Ni–R as well as the first iron-hydride-related NRVS feature observed for a biological system. Although density function theory (DFT) calculation assisted the determination of the Ni–R structure, it did not predict the Ni–H–Fe wag mode at ∼675 cm−1 before NRVS. Instead, the observed Ni–H–Fe mode provided a critical reference for the DFT calculations. While the overall science about Ni–R is presented and discussed elsewhere, this article focuses on the long and strenuous experimental journey to search for and experimentally identify the Ni–H–Fe wag mode in a Ni–R sample. As a methodology, the results presented here will go beyond Ni–R and hydrogenase research and will also be of interest to other scientists who use synchrotron radiation for measuring dilute samples or weak spectroscopic features.
- Published
- 2015
78. Roles of charged residues in pH-dependent redox properties of cytochrome c3 from Desulfovibrio vulgaris Miyazaki F
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Kiyoshi Ozawa, Hirofumi Komori, Naoki Yahata, Yusuke Tomimoto, Yoshiki Higuchi, Hideaki Ogata, Kumiko Morita, and Hideo Akutsu
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Hydrogenase ,biology ,Chemistry ,Stereochemistry ,nuclear magnetic resonance (NMR) ,Inorganic chemistry ,Biophysics ,Protonation ,Articles ,biology.organism_classification ,electron transfer ,Electron transport chain ,Redox ,tetraheme protein ,Electron transfer ,chemistry.chemical_compound ,Deprotonation ,hydrogenase ,site-directed mutagenesis ,Desulfovibrio vulgaris ,Heme - Abstract
Complicated pH-properties of the tetraheme cytochrome c3 (cyt c3) from Desulfovibrio vulgaris Miyazaki F (DvMF) were examined by the pH titrations of 1H-15N HSQC spectra in the ferric and ferrous states. The redox-linked pKa shift for the propionate group at C13 of heme 1 was observed as the changes of the NH signals around it. This pKa shift is consistent with the redox-linked conformational alteration responsible for the cooperative reduction between hemes 1 and 2. On the other hand, large chemical shift changes caused by the protonation/deprotonation of Glu41 and/or Asp42, and His67 were redox-independent. Nevertheless, these charged residues affect the redox properties of the four hemes. Furthermore, one of interesting charged residues, Glu41, was studied by site-directed mutagenesis. E41K mutation increased the microscopic redox potentials of heme 1 by 46 and 34 mV, and heme 2 by 35 and 30 mV at the first and last reduction steps, respectively. Although global folding in the crystal structure of E41K cyt c3 is similar to that of wild type, local change was observed in 1H NMR spectrum. Glu41 is important to keep the stable conformation in the region between hemes 1 and 2, controlling the redox properties of DvMF cyt c3. In contrast, the kinetic parameters for electron transfer from DvMF [NiFe] hydrogenase were not influenced by E41K mutation. This suggests that the region between hemes 1 and 2 is not involved in the interaction with [NiFe] hydrogenase, and it supports the idea that heme 4 is the exclusive entrance gate to accept the electron in the initial reduction stage.
- Published
- 2006
79. Redox Interaction of Cytochromec3with [NiFe] Hydrogenase fromDesulfovibrio vulgarisMiyazaki F
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Naoki Yahata, Yoshiki Higuchi, Takashi Saitoh, Kiyoshi Ozawa, Hideaki Ogata, Hideo Akutsu, and Yuki Takayama
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Models, Molecular ,Magnetic Resonance Spectroscopy ,Hydrogenase ,Stereochemistry ,Cytochrome c Group ,Heme ,Photochemistry ,Biochemistry ,chemistry.chemical_compound ,Methionine ,Cytochrome C1 ,Cytochrome c oxidase ,Desulfovibrio vulgaris ,Binding Sites ,biology ,Cytochrome c peroxidase ,Chemistry ,Cytochrome c ,biology.organism_classification ,Kinetics ,Coenzyme Q – cytochrome c reductase ,biology.protein ,Oxidation-Reduction - Abstract
Cytochrome c3 isolated from a sulfate-reducing bacterium, Desulfovibrio vulgaris Miyazaki F, is a tetraheme protein. Its physiological partner, [NiFe] hydrogenase, catalyzes the reversible oxidoreduction of molecular hydrogen. To elucidate the mechanism of electron transfer between cytochrome c3 and [NiFe] hydrogenase, the transient complex formation by these proteins was investigated by means of NMR. All NH signals of uniformly 15N-labeled ferric cytochrome c3 except N-terminus, Pro, and Gly73 were assigned. 1H-15N HSQC spectra were recorded for 15N-labeled ferric and ferrous cytochrome c3, in the absence and presence of hydrogenase. Chemical shift perturbations were observed in the region around heme 4 in both oxidation states. Additionally, the region between hemes 1 and 3 in ferrous cytochrome c3 was affected in the presence of hydrogenase, suggesting that the mode of interaction is different in each redox state. Heme 3 is probably the electron gate for ferrous cytochrome c3. To investigate the transient complex of cytochrome c3 and hydrogenase in detail, modeling of the complex was performed for the oxidized proteins using a docking program, ZDOCK 2.3, and NMR data. Furthermore, the roles of lysine residues of cytochrome c3 in the interaction with hydrogenase were investigated by site-directed mutagenesis. When the lysine residues around heme 4 were replaced by an uncharged residue, methionine, one by one, the Km of the electron-transfer kinetics increased. The results showed that the positive charges of Lys60, Lys72, Lys95, and Lys101 around heme 4 are important for formation of the transient complex with [NiFe] hydrogenase in the initial stage of the cytochrome c3 reduction. This finding is consistent with the most possible structure of the transient complex obtained by modeling.
- Published
- 2006
80. Activation Process of [NiFe] Hydrogenase Elucidated by High-Resolution X-Ray Analyses: Conversion of the Ready to the Unready State
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Hideaki Ogata, Yoshiki Higuchi, Naoki Shibata, Kenji Kano, Tatsuhisa Kato, Hirofumi Komori, A. Nakahara, and Shun Hirota
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Hydrogenase ,biology ,Inorganic chemistry ,Electron Spin Resonance Spectroscopy ,Active site ,chemistry.chemical_element ,Bridging ligand ,Crystal structure ,Sulfides ,Crystallography, X-Ray ,Diatomic molecule ,Protein Structure, Tertiary ,Enzyme Activation ,Crystallography ,Monatomic ion ,Nickel ,chemistry ,Structural Biology ,Catalytic Domain ,biology.protein ,Desulfovibrio vulgaris ,Energy source ,Molecular Biology - Abstract
SummaryHydrogenases catalyze oxidoreduction of molecular hydrogen and have potential applications for utilizing dihydrogen as an energy source. [NiFe] hydrogenase has two different oxidized states, Ni-A (unready, exhibits a lag phase in reductive activation) and Ni-B (ready). We have succeeded in converting Ni-B to Ni-A with the use of Na2S and O2 and determining the high-resolution crystal structures of both states. Ni-B possesses a monatomic nonprotein bridging ligand at the Ni-Fe active site, whereas Ni-A has a diatomic species. The terminal atom of the bridging species of Ni-A occupies a similar position as C of the exogenous CO in the CO complex (inhibited state). The common features of the enzyme structures at the unready (Ni-A) and inhibited (CO complex) states are proposed. These findings provide useful information on the design of new systems of biomimetic dihydrogen production and fuel cell devices.
- Published
- 2005
81. Blastoid Variant of Mantle Cell Lymphoma with Lactic Acidosis: A Case Report
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Kazuaki Yakushiji, Rie Imamura, Michio Sata, Michitoshi Hashiguchi, Ritsuko Seki, Hideaki Ogata, Koji Yoshimoto, Korenori Ohtsubo, Koichi Ohshima, and Takashi Okamura
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Male ,Pathology ,medicine.medical_specialty ,Antineoplastic Agents ,Lymphoma, Mantle-Cell ,Blastoid ,Antibodies, Monoclonal, Murine-Derived ,Antigens, CD ,Bone Marrow ,immune system diseases ,hemic and lymphatic diseases ,medicine ,Humans ,Lymphatic Diseases ,Aged ,biology ,business.industry ,Antibodies, Monoclonal ,Hematology ,medicine.disease ,biology.organism_classification ,Lymphoma ,Radiography ,medicine.anatomical_structure ,Lactic acidosis ,Splenomegaly ,Acidosis, Lactic ,Drug Therapy, Combination ,Rituximab ,Mantle cell lymphoma ,Bone marrow ,CD5 ,Severe lactic acidosis ,business ,medicine.drug - Abstract
Approximately 20% of mantle cell lymphomas (MCL) present with the blastoid variant associated with poor prognosis. Lactic acidosis complicated with hematologic malignancies is seen infrequently and is associated with a poor outcome. Here we report the case of a patient with the blastoid variant of MCL complicated by lactic acidosis and who achieved complete remission through chemotherapy combined with rituximab therapy. A 77-year-old man presented with peripheral blood lymphoma cells, huge splenomegaly, abdominal and mediastinal lymphadenopathy, and pleural effusion. A bone marrow smear showed an increase in large, abnormal lymphoid cells with oval or round nuclei, distinct nucleoli, and abundant basophilic cytoplasm with vacuolization. Splenic sections also showed massive and diffuse infiltration by these cells. Flow cytometry analysis showed these cells to be positive for CD5, CD19, CD20, and kappa chain and negative for CD10 and CD23. A blastoid variant of MCL was diagnosed from the results of histologic, immunohistochemical (cyclin D1), and cytogenetic (chimeric bcl-1/IgH fusion gene) analyses. The patient recovered from the 2 episodes of severe lactic acidosis for which he had been given chemotherapy, and he achieved complete remission after subsequent chemotherapy combined with rituximab treatment.
- Published
- 2004
82. A Case of Tubular Carcinoma of the Breast
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Hitoshi Murakuni, Nobuo Okuyama, Hideaki Ogata, Shigeharu Hamatani, and Tadaaki Shiba
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Oncology ,medicine.medical_specialty ,Pathology ,business.industry ,Internal medicine ,medicine ,Tubular carcinoma ,business - Published
- 2004
83. Role of the Aromatic Ring of Tyr43 in Tetraheme Cytochrome c3 from Desulfovibrio vulgaris Miyazaki F
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Fumiko Yasukawa, Yusuke Tomimoto, Tomoaki Ohmura, Michael A. Cusanovich, Kiyoshi Ozawa, Yoshiki Higuchi, Hideo Akutsu, Yuki Takayama, and Hideaki Ogata
- Subjects
Models, Molecular ,Semiquinone ,Protein Conformation ,Stereochemistry ,Biophysics ,Cytochrome c Group ,Heme ,Bioenergetics ,Hydrocarbons, Aromatic ,Redox ,Structure-Activity Relationship ,chemistry.chemical_compound ,Electron transfer ,Enzyme Stability ,Computer Simulation ,Desulfovibrio vulgaris ,Tyrosine ,Photolysis ,biology ,Ligand ,Lasers ,biology.organism_classification ,Electron transport chain ,Recombinant Proteins ,Enzyme Activation ,Amino Acid Substitution ,chemistry ,Mutagenesis, Site-Directed ,Oxidation-Reduction - Abstract
Tyrosine 43 is positioned parallel to the fifth heme axial ligand, His34, of heme 1 in the tetraheme cytochrome c3. The replacement of tyrosine with leucine increased the redox potential of heme 1 by 44 and 35mV at the first and last reduction steps, respectively; its effects on the other hemes are small. In contrast, the Y43F mutation hardly changed the potentials. It shows that the aromatic ring at this position contributes to lowering the redox potential of heme 1 locally, although this cannot be the major contribution to the extremely low redox potentials of cytochrome c3. Furthermore, temperature-dependent line-width broadening in partially reduced samples established that the aromatic ring at position 43 participates in the control of the kinetics of intramolecular electron transfer. The rate of reduction of Y43L cytochrome c3 by 5-deazariboflavin semiquinone under partially reduced conditions was significantly different from that of the wild type in the last stage of the reduction, supporting the involvement of Tyr43 in regulation of reduction kinetics. The mutation of Y43L, however, did not induce a significant change in the crystal structure.
- Published
- 2003
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84. Possible mechanisms of serotonin and aprepitant actions in chemotherapy induced nausea and vomiting (CINV): Insights into the mechanisms of serotonin and aprepitant actions in CINV—According to recent multi-institutional double-blind randomized clinical research on the AC regimen
- Author
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Hideaki Ogata, Goro Kutomi, Michiko Tsuneizumi, Joe Matsuoka, Keiko Hosoya, Mitsue Saito, and Yuko Kawai
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Oncology ,Cancer Research ,medicine.medical_specialty ,business.industry ,medicine.drug_class ,Palonosetron ,Granisetron ,AC Regimen ,Clinical research ,Internal medicine ,medicine ,Antiemetic ,Serotonin ,business ,Aprepitant ,medicine.drug ,Chemotherapy-induced nausea and vomiting - Abstract
6587 Background: One of our interests has been whether palonosetron(P) would be superior to granisetron(G) when administering triplet antiemetic therapy for the prevention of CINV, since a prior trial demonstrated P to be superior to G for controlling CINV induced by highly emetogenic chemotherapy (HEC) in doublet therapy. In this study(TTT; trial for antiemetic therapy), we assessed the efficacies of P and G for use as triplet antiemetic therapy for AC, by monitoring CINV, focusing complete response (CR; no vomiting and no rescue medicine) in the delayed phase. The primary endpoint of TTT was a CR during the delayed phase with 5-HT3ra plus dexamethasone and aprepitant administration for AC. The purpose of gaining insights into the possible mechanism of action of aprepitant and P was to obtain ideas for the next strategy against CINV. Methods: Between 2012 and 2015, 491 breast cancer receiving AC were recruited from 11 institutions, and randomly assigned to either single-dose P(0.75mg) or G(40μg/kg) prior to AC on day 1, both with dexamethasone (9.9 mg) and aprepitant (125mg) on day 1 followed by additional doses (80mg) on days 2 and 3. Age, institution and habitual alcohol intake were used as stratification factors. The primary endpoint was a CR. Results: All 491 patients were included in efficacy analyses: 246 patients in the group P and 245 in the group G. The difference in CR during the delayed phase, i.e. 24 hrs after the administration of AC, did not reach statistical significance, however, there was a remarkable difference between 48 and 72 hrs in the day-to-day analysis(p < 0.02). Conclusions: P showed better efficacy in controlling CINV between 48 to 72 hours after AC, than G as triplet antiemetic therapy for AC. We can reasonably speculate that the influence of serotonin has two peaks (0-24 hrs and 48-72 hrs). For controlling CINV in the delayed phase, not only an NK1 receptor antagonist but also administering a 5-HT3ra with long life should be considered until 72 hrs after HEC. Clinical trial information: UMIN 000007882.
- Published
- 2017
85. Hydrogenases
- Author
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Wolfgang Lubitz, Hideaki Ogata, Olaf Rüdiger, and Edward Reijerse
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Hydrogenase ,Biomimetics ,Biofuels ,Electrochemistry ,General Chemistry ,Photochemical Processes - Published
- 2014
86. Annexin V inhibits the 12-O-tetradecanoylphorbol-13-acetate-induced activation of Ras/extracellular signal-regulated kinase (ERK) signaling pathway upstream of Shc in MCF-7 cells
- Author
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Hirokazu Sato, Luigi M. De Luca, and Hideaki Ogata
- Subjects
Cyclin-Dependent Kinase Inhibitor p21 ,MAPK/ERK pathway ,Cancer Research ,Biology ,12-O-Tetradecanoylphorbol-13-acetate ,chemistry.chemical_compound ,Annexin ,Cyclins ,Tumor Cells, Cultured ,Genetics ,Humans ,Annexin A5 ,Enzyme Inhibitors ,Phosphorylation ,Kinase activity ,Protein kinase A ,Molecular Biology ,Protein Kinase C ,Protein kinase C ,Cell biology ,chemistry ,Tetradecanoylphorbol Acetate ,Mitogen-Activated Protein Kinases ,Signal transduction ,Annexin A2 ,Signal Transduction - Abstract
Annexin V is a Ca2+-dependent phospholipid binding protein. Although it has been shown to inhibit protein kinase C (PKC) in cell-free systems, its role in the intact cell is unclear. A stable MCF-7 human breast cancer cell overexpression system was established to investigate the function of annexin V. In these cells, 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced phosphorylation and kinase activity of ERK1/2 were suppressed. Morphological changes induced by TPA were reduced by annexin V overexpression as well as by the pan-PKC inhibitor, bisindolylmaleimide I, and by the mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK) inhibitor, PD98059. TPA-induced MEK1/2 and Raf-1 phosphorylation were reduced in these cells. The TPA-enhanced active Ras, and its association with Raf-1, were reduced. TPA treatment of MCF-7 cells caused an increased association of Shc with Grb2. However, this increased association was prevented in the annexin V-overexpressors. p21WAF/CIP1 is responsible for inhibition of cell cycle progression in MCF-7 cells. TPA induced the expression of p21WAF/CIP1 to a greater extent in MCF-7 parent and control plasmid cells than in annexin V overexpressors. PD98059 inhibited this increase, suggesting that TPA upregulation of p21WAF/CIP1 occurs via the MEK pathway, and that annexin V overexpression blunts it. This work shows that annexin V overexpression suppresses the TPA-induced Ras/ERK signaling by inhibiting at/or upstream of Shc, possibly through the inhibition of PKCs. Oncogene (2000).
- Published
- 2000
87. Crystallization and preliminary X-ray analysis of the small subunit (R2F) of native ribonucleotide reductase fromCorynebacterium ammoniagenes
- Author
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Georg Auling, Wolfgang Lubitz, Matthias Stehr, Hideaki Ogata, and Patrick Stolle
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Ribonucleotide ,Stereochemistry ,Protein subunit ,Biophysics ,Corynebacterium ,Biology ,Crystallography, X-Ray ,Biochemistry ,Fluorescence spectroscopy ,law.invention ,Crystal ,Structural Biology ,law ,Ribonucleotide Reductases ,Genetics ,Nucleotide ,Crystallization ,chemistry.chemical_classification ,Condensed Matter Physics ,enzymes and coenzymes (carbohydrates) ,Protein Subunits ,Crystallography ,Ribonucleotide reductase ,chemistry ,Crystallization Communications ,bacteria ,Single crystal - Abstract
Ribonucleotide reduction, the unique step in DNA-precursor biosynthesis, involves radical-dependent redox chemistry and diverse metallo-cofactors. The metallo-cofactor (R2F) encoded by the nrdF (nucleotide reduction) gene in Corynebacterium ammoniagenes ATCC 6872 was isolated after homologous expression and a new crystal form of ribonucleotide reductase R2F was obtained. R2F was crystallized at 277 K using the vapour-diffusion method with PEG as the precipitating agent. A data set was collected to 1.36 A resolution from a single crystal at 100 K using synchrotron radiation. The crystal belonged to space group C2, with unit-cell parameters a = 96.21, b = 87.68, c = 83.25 A, beta = 99.29 degrees. The crystal contained two molecules per asymmetric unit, with a Matthews coefficient (V(M)) of 2.69 A(3) Da(-1); the solvent content was estimated to be 54.3%. X-ray fluorescence spectroscopy and MAD diffraction data indicated the presence of manganese in the molecule and the absence of iron.
- Published
- 2009
88. Crystallization and preliminary X-ray analysis of the LOV domain of the blue-light receptor YtvA fromBacillus amyloliquefaciensFZB42
- Author
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Wolfgang Gärtner, Hideaki Ogata, Aba Losi, and Zhen Cao
- Subjects
DNA, Bacterial ,Light ,Bacillus amyloliquefaciens ,Protein Conformation ,Biophysics ,Bacillus ,Crystal structure ,Crystallography, X-Ray ,Biochemistry ,law.invention ,Crystal ,Protein structure ,Bacterial Proteins ,Structural Biology ,law ,Genetics ,Molecule ,Crystallization ,DNA Primers ,Base Sequence ,biology ,Chemistry ,Resolution (electron density) ,Condensed Matter Physics ,biology.organism_classification ,Crystallography ,Crystallization Communications ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Electrophoresis, Polyacrylamide Gel ,Single crystal - Abstract
Light-oxygen-voltage (LOV) proteins play an important role in blue-light-dependent physiological processes in many organisms. The LOV domain of the blue-light receptor YtvA from Bacillus amyloliquefaciens FZB42 has been purified and crystallized at 277 K using the sitting-drop vapour-diffusion method with 2-ethoxyethanol as a precipitant. A data set was collected to 1.60 A resolution from a single crystal at 100 K using synchrotron radiation. The LOV domain of YtvA crystallized in space group C222(1), with unit-cell parameters a = 64.95, b = 83.76, c = 55.81 A. The crystal structure of the LOV domain of YtvA was determined by the molecular-replacement method. The crystal contained one molecule per asymmetric unit, with a Matthews coefficient (V(M)) of 3.04 A(3) Da(-1); the solvent content was estimated to be 59.5%.
- Published
- 2009
89. Removal of the bridging ligand atom at the Ni–Fe active site of [NiFe] hydrogenase upon reduction with H2, as revealed by X-ray structure analysis at 1.4 Å resolution
- Author
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Noritake Yasuoka, Kunio Miki, Tatsuhiko Yagi, Yoshiki Higuchi, and Hideaki Ogata
- Subjects
Models, Molecular ,Binding Sites ,Hydrogenase ,biology ,Protein Conformation ,Ligand ,Iron ,chemistry.chemical_element ,Active site ,Bridging ligand ,Crystal structure ,Crystallography, X-Ray ,Ligands ,biology.organism_classification ,Sulfur ,Crystallography ,Monatomic ion ,chemistry ,Nickel ,Structural Biology ,biology.protein ,Desulfovibrio vulgaris ,Molecular Biology ,Hydrogen - Abstract
Background: The active site of [NiFe] hydrogenase, a heterodimeric protein, is suggested to be a binuclear Ni–Fe complex having three diatomic ligands to the Fe atom and three bridging ligands between the Fe and Ni atoms in the oxidized form of the enzyme. Two of the bridging ligands are thiolate sidechains of cysteinyl residues of the large subunit, but the third bridging ligand was assigned as a non-protein monatomic sulfur species in Desulfovibrio vulgaris Miyazaki F hydrogenase. Results: The X-ray crystal structure of the reduced form of D. vulgaris Miyazaki F [NiFe] hydrogenase has been solved at 1.4 A resolution and refined to a crystallographic R factor of 21.8%. The overall structure is very similar to that of the oxidized form, with the exception that the third monatomic bridge observed at the Ni–Fe site in the oxidized enzyme is absent, leaving this site unoccupied in the reduced form. Conclusions: The unusual ligand structure found in the oxidized form of D. vulgaris Miyazaki F [NiFe] hydrogenase was confirmed in the reduced form of the enzyme, with the exception that the electron density assigned to the monatomic sulfur bridge had almost disappeared. On the basis of this finding, as well as the observation that H 2 S is liberated from the oxidized enzyme under an atmosphere of H 2 in the presence of its electron carrier, it was postulated that the monatomic sulfur bridge must be removed for the enzyme to be activated. A possible mechanism for the catalytic action of the hydrogenase is proposed.
- Published
- 1999
90. 2070180 Relation Between Effect of Neoadjuvant Chemotherapy in Breast Cancer Patients and Changes of Time to Peak Using Contrast-Enhanced Ultrasound
- Author
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Yukio Mitsuzuka, Yorichika Kubota, Hideaki Ogata, Shinsaku Kanazawa, Akemi Kataoka, Toshihide Ito, Tadatoshi Osaku, Fumi Saito, and Shunsuke Magoshi
- Subjects
Oncology ,medicine.medical_specialty ,Chemotherapy ,Acoustics and Ultrasonics ,Radiological and Ultrasound Technology ,business.industry ,medicine.medical_treatment ,Biophysics ,medicine.disease ,Breast cancer ,Internal medicine ,medicine ,Time to peak ,Radiology, Nuclear Medicine and imaging ,business ,Contrast-enhanced ultrasound - Published
- 2015
91. Measurement of Reticulated Platelets in Thrombocytopenia. Methodology and Clinical Utility
- Author
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Hideaki Ogata
- Subjects
Adult ,Blood Platelets ,Male ,medicine.medical_specialty ,Cirrhosis ,business.industry ,Reticulated platelets ,General Medicine ,Middle Aged ,medicine.disease ,Thiazole orange ,Thrombocytopenia ,Gastroenterology ,Thrombocytopenic purpura ,Surgery ,Predictive Value of Tests ,Case-Control Studies ,Internal medicine ,medicine ,Humans ,Female ,Thrombopoiesis ,business ,Aged - Abstract
In this study, we explored the methodology and clinical utility of reticulated platelets analysis. Reticulated platelets were measured using flow cytometry in peripheral circulation with thiazole orange (TO). We chose fixed platelet-rich plasma as the sample since it has been known to give stable results. The percentage of reticulated platelets in 7 patients with idiopathic thrombocytopenic purpura (ITP) was significantly increased (8.41 +/- 5.35%; mean +/- SD), compared with that in 21 normal subjects (1.92 +/- 1.27%). The value in 16 patients with liver cirrhosis was slightly below normal (1.86 +/- 0.85%). These findings suggested that thrombopoiesis was not promoted under the condition of hypersplenism or of abnormal pooling, and that any elevation in the percentage level of reticulated platelets could be clinically useful in the diagnosis of ITP.
- Published
- 1998
92. Contents Vol. 116, 2006
- Author
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Yurie Saitoh, Manuela Leo, Rie Imamura, Paola Carluccio, Raj Rangineni, Giorgina Specchia, Ritsuko Seki, Kazuei Ogawa, Ioannis Stefanidis, Ernest Beutler, Rívia Mara Morais e Silva, Theodoros Eleftheriadis, Christian Breymann, Michio Sata, Jonathan M. Flanagan, Georgia Antoniadi, Yukio Maruyama, Michitoshi Hashiguchi, Terri Gelbart, Tilo Burkhardt, Domenico Pastore, Hideaki Ogata, Arcangelo Liso, Rokuo Abe, Takashi Okamura, Maria Ditsa, Margherita Giannoccaro, Kohji Yoshimoto, Silvia Sibilla, Fernando Coutinho, Koichi Osaki, Maria das Graças Carvalho, Lei Wang, Ana Rita Manhani, Vassilios Liakopoulos, Eijiro Oku, Eiji Ando, Gabriela Bencaiova, Hiroaki Nagamatsu, Ashok Kumar Malani, Auro Del Giglio, Dimitra Markala, Anna Mestice, Margherita Casanova, Charalambos Kartsios, Pauline Lee, Tsutomu Shichishima, Vicram Gupta, Geraldo Majella Medeiros de Paula, Kazuaki Yakushiji, Vânia Maria Freitas, Hongfan Peng, G. Anifandis, André Aleixo Pereira Hipólito Dantas, Alexander Krafft, Vincenzo Liso, Luci Maria Sant'Ana Dusse, Barbra Sasu, Christos Papadopoulos, Aliki Skirta, Kazuhide Shimamatsu, Lauro Mello Vieira, and Korenori Ohtsubo
- Subjects
Hematology ,General Medicine - Published
- 2006
93. Phase II clinical trial of high-dose toremifene as primary hormone therapy in aromatase inhibitor-resistant breast cancer
- Author
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Hideaki, Ogata, Yasushi, Okamoto, Youichi, Arima, Hisaki, Fukushima, Hiroshi, Takeyama, Akinori, Yamashita, Masao, Kinoshita, Noriyuki, Suzuki, Terumasa, Sawada, Yoshitomo, Koshida, Akira, Matsui, Akihiko, Tachibana, Hiroshi, Nakayama, Yoko, Oishi, Hiroko, Nogi, and Ken, Uchida
- Subjects
Aged, 80 and over ,Antineoplastic Agents, Hormonal ,Aromatase Inhibitors ,Drug Resistance, Neoplasm ,Humans ,Breast Neoplasms ,Female ,Toremifene ,Middle Aged ,Aged - Abstract
Third-generation aromatase inhibitors(AIs)are now common in adjuvant hormone therapy for breast cancer in postmenopausal women. However, a suitable treatment has yet to be established for patients who develop cancer recurrence during or after adjuvant AI therapy.This prospective study evaluated the efficacy and safety of 120mg/day toremifene citrate(TOR-120)administered orally to 23 patients with recurrent breast cancer who were receiving or had received adjuvant AI therapy. Primary therapy for recurrence was TOR-120 monotherapy.The response rate was 13. 0%(partial response: three patients), the clinical benefit rate was 78. 3%(partial response: three patients; long-term stable disease: 15 patients), and median time to progression was 8. 1 months. Grade 1 adverse events such as loss of appetite, sweating, flushing and edema face were observed.TOR-120 monotherapy was effective and safe as a primary hormone therapy for recurrent breast cancer unresponsive to AIs.
- Published
- 2013
94. Hydrogenases, Structure and Function
- Author
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Wolfgang Lubitz and Hideaki Ogata
- Subjects
Hydrogenase ,biology ,Iron–sulfur cluster ,chemistry.chemical_element ,Active site ,Photochemistry ,Heterolysis ,chemistry.chemical_compound ,Nickel ,Crystallography ,Electron transfer ,chemistry ,Catalytic cycle ,biology.protein ,Carbon monoxide - Abstract
Hydrogenase catalyzes the reversible oxidation of dihydrogen. They can be divided into three phylogenetically distinct classes, that is, [NiFe], [FeFe], and [Fe] hydrogenases, according to the type of catalytically active metal center. Whereas most [NiFe] hydrogenases are of the hydrogen uptake type, [FeFe] hydrogenases mainly produce molecular hydrogen and [Fe] hydrogenases catalyze a specific reaction utilizing H 2 . Crystallographic structures of hydrogenases from all three classes have been determined at atomic resolution and have given insight into the possible pathways for the transfer of electrons, protons, and gaseous molecules such as dihydrogen. In [NiFe] and [FeFe] hydrogenases, the electrons are transferred via chains of iron–sulfur centers, whereas the [Fe] hydrogenases have no iron–sulfur centers. All three classes of hydrogenases contain at least one iron atom in the active site, which carries unusual nonprotein diatomic ligands, such as carbon monoxide and cyanide. A common mechanism of all three types of hydrogenases for the heterolytic splitting of dihydrogen has been proposed that takes place at a dinuclear X-Fe center. The only variable is the first cation X which can be a nickel, an iron, or a carbon in the three classes of hydrogenases.
- Published
- 2013
95. Crystallization and preliminary X-ray analysis of two inhibitor complexes of the catalytic domain of death-associated protein kinase
- Author
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Akira Yamakawa, Hiromi Sanuki, Naoki Shibata, Yamada Kazunori, Naoko Andou, Kumiko Morita, Takeshi Hioki, Takehisa Ishii, Hideaki Ogata, and Yoshiki Higuchi
- Subjects
Crystallography, X-Ray ,Catalysis ,law.invention ,chemistry.chemical_compound ,Structural Biology ,law ,Catalytic Domain ,PEG ratio ,medicine ,Animals ,Humans ,Molecule ,Staurosporine ,Cloning, Molecular ,Enzyme Inhibitors ,Crystallization ,Protein kinase A ,PEG 400 ,Chemistry ,Magnesium acetate ,General Medicine ,Death-Associated Protein Kinases ,Crystallography ,Calcium-Calmodulin-Dependent Protein Kinases ,Apoptosis Regulatory Proteins ,medicine.drug - Abstract
The catalytic domain of death-associated protein kinase (DAPK) has been overexpressed, purified and crystallized using the sitting-drop vapour-diffusion method with PEG 8000 and magnesium acetate as precipitants. Complexes with the inhibitor staurosporine and its analogue BDB402 were also crystallized in the presence of PEG 400 and PEG 8000, respectively. Diffraction data were collected to 2.4 A for the native catalytic domain, to 2.9 A for the staurosporine complex and to 2.7 A for the BDB402 complex. All three crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 77.992, b = 109.909, c = 50.063 A for the catalytic domain, a = 78.911, b = 113.162, c = 50.658 A for the staurosporine complex and a = 77.337, b = 108.869, c = 50.186 A for the BDB402 complex. In both complexes the inhibitor molecule was clearly assigned in the difference Fourier map calculated on the basis of the phases obtained from the structure of the catalytic domain.
- Published
- 2004
96. Insertion of an H-bonding residue into the distal pocket of the ferriheme protein nitrophorin 4: effect on nitrite-iron coordination and nitrite disproportionation
- Author
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Markus Knipp, Chunmao He, and Hideaki Ogata
- Subjects
Hemeproteins ,Models, Molecular ,Hemeprotein ,Magnetic Resonance Spectroscopy ,Stereochemistry ,Iron ,Mutant ,Bioengineering ,Disproportionation ,Crystallography, X-Ray ,Biochemistry ,Residue (chemistry) ,chemistry.chemical_compound ,Nitrite ,Salivary Proteins and Peptides ,Molecular Biology ,Heme ,Histidine ,Nitrites ,Binding Sites ,Chemistry ,Hydrogen bond ,Hydrogen Bonding ,General Chemistry ,General Medicine ,Mutation ,Molecular Medicine ,Hemin - Abstract
Heme proteins are important entities for the metabolism of nitrite. Inspection of the structural features of the reported hemoprotein-nitrite crystal structures reveals that, except for nitrophorin 4 (NP4), H-bonding to the nitrite ligand is accomplished via histidine or arginine residues. These H-bonds probably play an important role for the nitrite coordination and/or reactivities. In nitrophorins, which catalyze the nitrite disproportionation reaction, such a residue is missing. Here, we report on the L130R mutant of the NP isoprotein NP4 that provides the Arg130 residue as part of the flexible G-H loop as a potential H-bonding residue in the distal heme pocket. Similar to the wild-type protein, nitrite remains N-bonded in the crystal structure of NP4(L130R). However, spectroscopic investigations show that, in solution, a second ligand-rotational orientation exists, which is in fast-exchange equilibrium with the normal, parallel ligand orientation. Moreover, the nitrite disproportionation is inhibited in NP4(L130R). Comparison with another, also less active mutant NP4(D30N) suggests that the displacement of H(2)O molecules from the heme cavity prevents the proton donation pathway through Asp30.
- Published
- 2012
97. [Remarkable improvement in a patient with metastatic and locally advanced HER2-positive breast cancer treated with trastuzumab plus vinorelbine]
- Author
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Shinsaku, Kanazawa, Hideaki, Ogata, Shunsuke, Magoshi, Fumi, Saito, Toshihide, Ito, Yorichika, Kubota, and Hironori, Kaneko
- Subjects
Adult ,Fatal Outcome ,Receptor, ErbB-2 ,Antineoplastic Combined Chemotherapy Protocols ,Humans ,Breast Neoplasms ,Female ,Vinorelbine ,Neoplasm Metastasis ,Trastuzumab ,Antibodies, Monoclonal, Humanized ,Tomography, X-Ray Computed ,Vinblastine - Abstract
A 39-year-old premenopausal nulliparous woman presented with severe pain in her right breast, bleeding and pus-like discharge, and a deep ulcer approximately 18 cm in diameter.Contralateral breast metastasis, bilateral axillary lymph node metastases, and multiple lung and bone metastases were detected on computed tomography.Five years previously she had undergone surgery for ovarian cancer and had prematurely discontinued adjuvant chemotherapy because of side effects. Following the administration of pain control, the patient received trastuzumab(Tr)plus vinorelbine(VNR)for her breast cancer as first-line therapy to avoid hair loss.The ulcer on her right chest wall underwent complete epithelialization and the patient's performance status improved from 3 to 0.The pus-like discharge, pain, bleeding, and odor from the breast resolved completely, and 5 months later, her quality of life had improved.The lung metastases also resolved completely.No adverse affects, including hematotoxicity and hair loss, were seen until treatment failure 12.5 months later. Second-line and third-line treatments were performed, but brain metastases developed, and the patient's overall condition deteriorated because of the development of ileus of unknown etiology.She died 21 months later.The patient received all therapies on an outpatient basis. Combination therapy using Tr and VNR is superior in safety and tolerability, and has been considered an option for first-line treatment of metastatic, locally advanced HER2-positive breast cancer.
- Published
- 2012
98. THREE CASES OF BONE-MARROW METASTASIS OF GASTRIC CANCER
- Author
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Mineo Wakabayashi, Yukio Tsugu, Hideaki Ogata, Shinichirou Matsuo, Chiaki Hashimura, Kyouei Nonaka, and Hiroko Nonaka
- Subjects
Bone marrow metastasis ,medicine.medical_specialty ,business.industry ,Cancer ,medicine.disease ,Low back pain ,Bone marrow cancer ,Metastasis ,Surgery ,Lesion ,Hematoma ,medicine.anatomical_structure ,Medicine ,Bone marrow ,medicine.symptom ,business - Abstract
The metastasis of gastric cancer to the bone marrow is relatively rate. This metastasis runs a course of rapid aggravation after manifestation and carries a very unfavorable prognosis. This paper describes three recent cases of spread of gastric cancer to the bone marrow, with selective reference to the literature. The patients were all men in their 50s. They all had low back pain, along with symptoms of hemorrhage such as hematuria and subdural hematoma before admission. At surgery the lesion of the gastric cancer was classified as IIc in one patient and Borr III in the remaining two patients. The interval surgery to the manifestation of the metastasis was about 10 years in the case of IIc, but was relatively short, at about one year or two, in the cases of Borr III. A definitive diagnosis of the results of hematological examination and bone scintigrams, besides the history and clinical symptoms, and the treatment was primarily concerned with the measures against hemorrhage. In all the patients of this seris the symptoms fatally exacerbated in the period of 19 to 34 days after the onset of bone marrow cancer. The metastasis of gastric cancer to the bone marrow generally manifests itself in about 5 years after surgery for the gastric cancer. The bone marrow cancer took 10 years to manifest itself in one of these patients. This metastasis is relatively easy to diagnosis, but there is no effective therapy available. What's more, its prognosis is very unfavorable. Accordingly, in the postoperative observation of patients with gastric cancer it is necessary to bear in mind the possibility of its spread to the bone marrow, and aggressive intervention in the lesion of metastasis is important.
- Published
- 1994
99. Structure and Function of Hydrogenase Enzymes
- Author
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Wolfgang Lubitz, Eduard J. Reijerse, Yoshiki Higuchi, and Hideaki Ogata
- Subjects
Hydrogenase ,Hydrogen ,biology ,Ligand ,Inorganic chemistry ,chemistry.chemical_element ,Active site ,Hydrogenase mimic ,Combinatorial chemistry ,Enzyme catalysis ,Catalysis ,Metal ,chemistry ,visual_art ,biology.protein ,visual_art.visual_art_medium - Abstract
The understanding of the basic principles of hydrogen production and utilization by the enzyme hydrogenase is a goal of major importance both for basic research and possible applications in our society. Hydrogenases are enzymes that facilitate the uptake and release of molecular hydrogen using a heterolytic reaction mechanism: H2⇌H++H−⇌2H++2e−. The acidity of H2, which is extremely low, is dramatically increased by binding to a metal. Many of the currently used catalysts for anthropogenic utilization of hydrogen involve precious metals such as platinum, while Nature's catalysts are based on cheap and abundant first row transition metals. Three phylogenetically distinct classes of hydrogenase are known; these are the [NiFe], the [FeFe] and the [Fe] hydrogenases. The first two classes have active sites containing binuclear metal cores with an unusual ligand sphere, whereas the third class harbors a mononuclear iron next to a special organic cofactor. In all these hydrogenases, the protein plays an important role for tuning the active site properties, but also by providing pathways for protons, electrons as well as dihydrogen. An important feature of the native systems is the very high turnover frequency (up to ∼104 s−1). Hydrogenases from (hyper)thermophilic organisms show a remarkable stability at high temperatures (up to ∼100°C) and several [NiFe] hydrogenases (e.g. from Knallgas bacteria) are active even in the presence of ambient levels of molecular oxygen. As discussed in this chapter, a combination of X-ray crystallography, spectroscopy, electrochemistry and quantum chemistry was instrumental in characterizing the hydrogenases with respect to their structure and function. Furthermore, mechanisms for the enzymatic reactions are proposed and guidelines for the construction of biomimetic hydrogenase model systems are provided.
- Published
- 2011
100. A CASE OF MORGAGNI HERNIA
- Author
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Masahiro Isogai, Hisatsugu Deguchi, Hideaki Ogata, Yukio Tsugu, Takeshi Asakura, and Kazuhiko Ohtaki
- Subjects
medicine.medical_specialty ,business.industry ,General surgery ,medicine ,Hernia ,business ,medicine.disease - Published
- 1993
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