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559 results on '"Heyndrickx M"'

56. The spoilage microbiota of ray (Raja sp.) during ice storage under different conditions: Molecular identification and characterisation of the spoilage potential

Author

Broekaert, K., Noseda, B., Heyndrickx, M., Vlaemynck, G., and Devlieghere, F.

Abstract

The dominant microbiota of ray stored on ice was systematically identified. Isolates grown on various media were identified by partial 16S rRNA, gyrB and rpoB gene sequencing. Microbiological shifts were observed during storage, ending in a dominance of especially members of the genera Pseudomonas and Psychrobacter. Most isolates could be identified by rpoB (Pseudomonas spp.) or gyrB gene sequencing as Pseudomonas fluorescens, Pseudomonas fragi, Pseudomonas psychrophila, Psychrobacter cibarius, Psychrobacter cryohalolentis, Psychrobacter glacincola and Psychrobacter immobilis. Also species from the genera Arthrobacter, Flavobacterium, Pseudoalteromonas, Shewanella and Staphylococcus were detected during storage of ray. Subsequently, the spoilage potential of six selected isolates (Flavobacterium tegetincola, Pseudomonas fluorescens, Pseudomonas psychrophila, Psychrobacter cibarius, Psychrobacter cryohalolentis and Shewanella frigidimarina) was determined and quantified based on the presence of VOCs. Additionally, API ZYM and urease analyses determined the species’ enzymatic capacity to contribute to spoilage by degrading lipids, amino acids and proteins and breaking down urea to ammonia. The six isolates were inoculated separately as pure cultures on gamma-sterilised ray. The inoculated samples were stored at 4°C and the production of VOCs by the pure strains on the ray matrix was identified via gas chromatography coupled to mass spectrometry (GC-MS). VOC production was quantified by selected ion flow tube mass spectrometry (SIFT-MS). The sensory profile of the selected species revealed that especially Psychrobacter cibarius and Pseudomonas psychrophila were able to produce higher concentrations of VOCs and might be responsible for the off-odours produced during spoilage of ray.

Published
2012

57. Volatile compounds associated with Psychrobacter spp. and Pseudoalteromonas spp., the dominant microbiota of brown shrimp (Crangon crangon) during aerobic storage

Author

Broekaert, K., Noseda, B., Heyndrickx, M., Vlaemynck, G., and Devlieghere, F.

Abstract

Psychrobacter and Pseudoalteromonas species dominate the microbiota of cooked brown shrimp (Crangon crangon). Therefore, the spoilage potential of several Psychrobacter and Pseudoalteromonas species (Psychrobacter cibarius, Psychrobacter maritimus, Pseudoalteromonas elyakovii, Pseudoalteromonas paragorgicola and Pseudoalteromonas nigrifaciens) was determined and quantified based on the presence of VOCs. Additionally, API ZYM analyses determined the species’ enzymatic capacity to contribute to spoilage by degrading lipids, amino acids and proteins. The bacterial species used in this study were isolated from cooked brown shrimp during storage (spoilage) under different storage and processing (peeled, unpeeled) conditions and were selected for analysis of their spoilage potential based on their difference in the (GTG)5-rep profile, 16S rRNA and gyrB sequences and API ZYM profile. The isolates were inoculated as pure cultures on heat-sterilised shrimp. The inoculated samples were stored at 4°C and the production of VOCs by the pure strains on the shrimp matrix was identified via gas chromatography coupled to mass spectrometry (GC-MS). VOC production was quantified daily by selected ion flow tube mass spectrometry (SIFT-MS) until the bacterial count exceeded 108 - 109 cfu/g. Based on the API ZYM results, Pseudoalteromonas as well as PsychrobacterPsychrobacter species revealed very low potential of the production of VOCs. Pseudoalteromonas species, especially Pseudoalteromonas elyakovii and Pseudoalteromonas nigrifaciens, produced significant amounts of volatile compounds such as sulphides, acetone, ammonia, and ethanol, which are all involved in seafood spoilage, and might be responsible for the off-odours produced during spoilage of brown shrimp.

Published
2012

64. Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria

Author

Logan, N. A., Berge, O., Bishop, A. H., Busse, H. J., De Vos, P., Fritze, D., Heyndrickx, M., Kämpfer, P., Rabinovitch, L., Ventosa Ucero, Antonio, and Universidad de Sevilla. Departamento de Microbiología y Parasitología

Abstract

Minimal standards for describing new taxa within the aerobic endospore-forming bacteria are proposed, following Recommendation 30b of the Bacteriological Code (1990 Revision). These minimal standards are recommended as guidelines to assist authors in the preparation of descriptions for novel taxa. They encourage broad polyphasic characterization and the construction of descriptions that are practically useful in routine diagnostic laboratories. The proposals have been endorsed by the Subcommittee on the Taxonomy of the Genus Bacillus and Related Organisms of the International Committee on Systematics of Prokaryotes.

Published
2009

65. SPI-2 of Salmonella typhimurium is not necessary for long term colonisation of pigs

Author

Haesebrouck, Freddy, Boyen, F., Volf, J., Botteldoorn, N., Adriaensen, Connie, Hernalsteens, Jean-Pierre, Ducatelle, Richard, Van Immerseel, F., Heyndrickx, M., Pasmans, Frank, and Viral Genetics

Subjects
Salmonella typhimurium, pig, hemic and lymphatic diseases, mutant, deletion, SPI-2
Abstract

Defined Salmonella mutants defective in the expression of genes in the pathogenicity island 2 are still able to colonize pigs.

Published
2007

71. Campylobacter in het vizier

Author

Heyndrickx, M. and Heyndrickx, M.

Abstract

Elk jaar worden zowat 200.000 Europeanen ernstig ziek door met campylobacter besmet voedsel te eten. ILVO heeft deze bacterie al een tijdje in het vizier, maar voorlopig blijven allesomvattende beheersingsstrategieën uit. Oplossingen die wel in het laboratorium lijken te werken, doen dit nog niet in de darm van de kip.

Published
2013

72. Dossier MRSA in de varkenshouderij

Author

Butaye, P., Crombé, F., Debleecker, Y., De Man, I., Delputte, P., Heyndrickx, M., Pletinckx, L., Rasschaert, G., Verhegghe, M., Butaye, P., Crombé, F., Debleecker, Y., De Man, I., Delputte, P., Heyndrickx, M., Pletinckx, L., Rasschaert, G., and Verhegghe, M.

Abstract

Drie artikelen over MRSA in de varkenshouderij: 1) Situatie op Vlaamse bedrijven. 2) Hoe verloopt de besmetting en verspreiding van MRSA? 3) Helpt reinigen en ontsmetten?

Published
2012

74. Koudeminnende pseudomonasbacteriën in rauwe melk

Author

De Jonghe, V., Heyndrickx, M., De Jonghe, V., and Heyndrickx, M.

Abstract

Koudeminnende bacteriën kunnen zich vermenigvuldigen in gekoelde rauwe melk. Omdat bederfenzymen de kwaliteit van de melk kunnen aantasten, moeten we de uitgroei van deze bacteriën zo veel mogelijk beperken.

Published
2011

75. Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria

Author

Universidad de Sevilla. Departamento de Microbiología y Parasitología, Logan, N. A., Berge, O., Bishop, A. H., Busse, H. J., De Vos, P., Fritze, D., Heyndrickx, M., Kämpfer, P., Rabinovitch, L., Ventosa Ucero, Antonio, Universidad de Sevilla. Departamento de Microbiología y Parasitología, Logan, N. A., Berge, O., Bishop, A. H., Busse, H. J., De Vos, P., Fritze, D., Heyndrickx, M., Kämpfer, P., Rabinovitch, L., and Ventosa Ucero, Antonio

Abstract

Minimal standards for describing new taxa within the aerobic endospore-forming bacteria are proposed, following Recommendation 30b of the Bacteriological Code (1990 Revision). These minimal standards are recommended as guidelines to assist authors in the preparation of descriptions for novel taxa. They encourage broad polyphasic characterization and the construction of descriptions that are practically useful in routine diagnostic laboratories. The proposals have been endorsed by the Subcommittee on the Taxonomy of the Genus Bacillus and Related Organisms of the International Committee on Systematics of Prokaryotes.

Published
2009

76. Phage and MLVA Typing of Salmonella Enteritidis Isolated from Layers and Humans in Belgium from 2000–2010, A Period in which Vaccination of Laying Hens was Introduced

Author

Dewaele, I., primary, Heyndrickx, M., additional, Rasschaert, G., additional, Bertrand, S., additional, Wildemauwe, C., additional, Wattiau, P., additional, Imberechts, H., additional, Herman, L., additional, Ducatelle, R., additional, Van Weyenberg, S., additional, and De Reu, K., additional

Published
2013
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85. Polyphasic characterization of Salmonella Enteritidis isolates on persistently contaminated layer farms during the implementation of a national control program with obligatory vaccination: A longitudinal study

Author

Dewaele, I., primary, Rasschaert, G., additional, Wildemauwe, C., additional, Van Meirhaeghe, H., additional, Vanrobaeys, M., additional, De Graef, E., additional, Herman, L., additional, Ducatelle, R., additional, Heyndrickx, M., additional, and De Reu, K., additional

Published
2012
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