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53. The predictive value of RNA binding proteins in colon adenocarcinoma

Author

Luan, Lipeng, primary, Lu, Feng, additional, Wang, Xiaochuan, additional, Wang, Yunliang, additional, Wang, Wei, additional, Yang, Yong, additional, Chen, Guoliang, additional, Yao, Huihui, additional, Shi, Xinyu, additional, Yuan, Zihan, additional, Zhou, Guoqiang, additional, Zhang, Haitao, additional, and He, Songbing, additional

Published
2021
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54. DAB2IP decreases cell growth and migration and increases sensitivity to chemotherapeutic drugs in colorectal cancer

Author

Wu, Guanting, primary, Xu, Xin, additional, Wan, Daiwei, additional, Zhou, Diyuan, additional, Feng, Yuan, additional, Chen, Junjie, additional, Peng, Zhijian, additional, Fang, Dong, additional, Shi, Xinyu, additional, Yao, Huihui, additional, Chen, Guoliang, additional, Sun, Liang, additional, Yao, Yizhou, additional, Zhou, Guoqiang, additional, Yang, Yili, additional, and He, Songbing, additional

Published
2021
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56. 2-Hydroxylation of Fatty Acids Represses Colorectal Tumorigenesis and Metastasis via the YAP Transcriptional Axis

Author

Sun, Liang, primary, Yang, Xiaoqin, additional, Huang, Xiaoheng, additional, Yao, Yizhou, additional, Wei, Xiangyu, additional, Yang, Shugao, additional, Zhou, Diyuan, additional, Zhang, Wei, additional, Long, Zhimin, additional, Xu, Xiaoyan, additional, Zhu, Xinguo, additional, He, Songbing, additional, and Su, Xiong, additional

Published
2021
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57. An Artificial Intelligence System to Predict Pathologic Response Using Longitudinal MRI in Locally Advanced Rectal Cancer: A Multicenter Study

Author

Ke, Jia, primary, Jin, Cheng, additional, Ding, Peirong, additional, Wu, Yuankui, additional, He, Songbing, additional, Tang, Jinghua, additional, Jiang, Xiaofeng, additional, Zhao, Hengyu, additional, Cao, Wuteng, additional, Zhou, Jie, additional, Chen, Guoliang, additional, Pang, Xiaolin, additional, Meng, Xiaochun, additional, Gao, Feng, additional, Li, Ruijiang, additional, Lan, Ping, additional, and Wu, Xiao-jian, additional

Published
2021
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58. Surgeons' fear of getting infected by COVID19: A global survey

Author

An, Yongbo, primary, Bellato, Vittoria, additional, Konishi, Tsuyoshi, additional, Pellino, Gianluca, additional, Sensi, Bruno, additional, Siragusa, Leandro, additional, Franceschilli, Marzia, additional, Sica, Giuseppe S, additional, Kefleyesus, A, additional, Hoofwijk, A G M, additional, Eldaly, Abdullah Sami, additional, Gonzalez, Abel, additional, Jawad, Adraoui, additional, Jooma, Ahmad, additional, Hafez, Ahmed Mousa, additional, Rubio, Ainhoa Valle, additional, Landaluce-Olavarria, Aitor, additional, Wu, Aiwen, additional, Nagatsu, Akihisa, additional, Inoue, Akira, additional, Kanamoto, Akira, additional, Ouchi, Akira, additional, El-Hussuna, Alaa, additional, Vazquez-Melero, Alba, additional, Wolthuis, Albert M, additional, Peral, Alberto M, additional, Lozano, Alejandra Cruz, additional, Efremov, Aleksandr, additional, Ryasantsev, Aleksandr V, additional, Di Giorgio, Alessandra, additional, Parente, Alessandro, additional, Tamburrini, Alessandro, additional, Alò, Alessio, additional, Forero-Torres, Alexander, additional, Vahrmeijer, Alexander L, additional, Varabei, Alexander, additional, Hinojosa, Siles, additional, Balkan, Ali Zeynel Abidin, additional, Frontali, Alice, additional, Oleg, Alikin, additional, Soler-Silva, Álvaro, additional, Makni, Amin, additional, André, Ana, additional, Cabrera, Ana María García, additional, Fernández, Ana María Gonzalez, additional, Minaya-Bravo, Ana M, additional, Rodríguez-Sánchez, Ana, additional, Musina, Ana-Maria, additional, Pangeni, Anang, additional, Zolotko, Anastasia, additional, Tonoyan, Andranik, additional, Balla, Andrea, additional, Belli, Andrea, additional, Cavallaro, Andrea, additional, Chierici, Andrea, additional, Divizia, Andrea, additional, Bucci, Andrea Fares, additional, Salido, Andrea Jiménez, additional, Morini, Andrea, additional, Muratore, Andrea, additional, Vignali, Andrea, additional, Chitul, Andrei, additional, Sebastian, Diaconescu Andrei, additional, Pcolkins, Andrejs, additional, Shchegolev, Andrey, additional, Hollenbeck, Andrew, additional, Wisneski, Andrew, additional, Iossa, Angelo, additional, D'Amore, Anna, additional, Hunter, Anndrew, additional, Hesketh, Anthony J, additional, La Brocca, Andrea, additional, Spinelli, Antonino, additional, Caires, Antonio, additional, D'Alessandro, Antonio, additional, Correo, Antonio Francisco Sanchís López, additional, Macrì, Antonio, additional, Navarro-Sánchez, Antonio, additional, Pronk, Apollo, additional, Akunc, Aram, additional, Mehri, Arash, additional, Pelta, Arie, additional, Papadopoulos, Aristeidis, additional, Kechagias, Aristotelis, additional, Rashid, Arshad, additional, Ramazanov, Artur, additional, Chandio, Ashfaq, additional, Kohyama, Atsushi, additional, Nishimura, Atsushi, additional, Ohkawa, Atsushi, additional, Dulskas, Audrius, additional, Jamal, Aun, additional, Mariani, Aurora, additional, Unal, Ayse Gizem, additional, Karagoz, Ayse, additional, Ozkan, Bahar Busra, additional, Salih, Barham, additional, Gülcü, Baris, additional, Pessia, Beatrice, additional, Martin-Perez, Beatriz, additional, Ielpo, Benedetto, additional, Tulelli, Berenice, additional, Yang, Bin, additional, Mhamed, Boumadani, additional, Murphy, Brenda, additional, Pirozzi, Brunella Maria, additional, Langenhoff, Bs, additional, Belevi, Bulent, additional, Güney, Burak, additional, Ng, Caecilia, additional, Rueda, Camilo, additional, Roxburgh, Campbell S, additional, Feo, Carlo V, additional, Ferrari, Carlo, additional, Gazia, Carlo, additional, Pratesi, Carlo, additional, Ratto, Carlo, additional, Santacruz, Carlos Cerdán, additional, Arroyave, Carlos Rodolfo Martinez, additional, Macias, Carlos, additional, Fernandez, Carlota Garcia, additional, Fernandez, Carmen Cagigas, additional, Curtis-Martinez, Carolina, additional, Fortmann, Caroline, additional, Kim, Caroline, additional, Galeano, Catalina Uribe, additional, Barroso, Catarina, additional, Baldi, Caterina, additional, Foppa, Caterina, additional, Formisano, Cesare, additional, Li, Changzai, additional, Ding, Chao, additional, Wang, Chenyu, additional, Iacusso, Chiara, additional, Yang, Chongwei, additional, Pizzera, Christian, additional, Skias, Christoph, additional, Chouliras, Christos, additional, Liakos, Christos, additional, Matsuda, Chu, additional, Wu, Chun-yi, additional, Ozaslan, Cihangir, additional, Tanda, Cinzia, additional, Tommaso, Cipolat Mis, additional, Dagorno, Claire, additional, Ramos, Claudia Patricia Arellano, additional, Arcudi, Claudio, additional, Coco, Claudio, additional, Morales, Cleotilde Mateo, additional, Ali, Mujahid Zulfiqar, additional, Lozano, Coral Cózar, additional, Sala, Corrado, additional, Leo, Cosimo Alex, additional, Scarpa, Cosimo Riccardo, additional, Ferro, Cristian Varela, additional, Fernandez, Cristina Mosquera, additional, Morales-Garcia, D, additional, Nakano, Daisuke, additional, Cristian, Daniel, additional, Hechtl, Daniel, additional, Cánovas, Daniel Triguero, additional, Calabrese, Daniela, additional, Rega, Daniela, additional, Ferraro, Daniele, additional, Morezzi, Daniele, additional, Sommacale, Daniele, additional, Brogden, Danielle, additional, Miskovic, Danilo, additional, Merlini, David, additional, Pertile, Davide, additional, Coniglio, Denise, additional, Zhu, Dexiang, additional, Wu, Dianwen, additional, Coletta, Diego, additional, Rubio, Diego Ramos, additional, Sasia, Diego, additional, Fillipov, Dmitry, additional, Russiello, Domenico, additional, Dardanov, Dragomir, additional, Consten, E C J, additional, Smolskas, Edgaras, additional, Muttillo, Edoardo Maria, additional, Jones, Edward, additional, Sunami, Eiji, additional, Etienne, El-Helou, additional, Chalkiadaki, Elena, additional, Giacomelli, Elena, additional, Karbovnichaya, Elena, additional, Ruiz-Úcar, Elena, additional, Guaitoli, Eleonora, additional, Samadov, Elgun, additional, Jovine, Elio, additional, Treppiedi, Elio, additional, Vaterlini, Elisa Maria, additional, Zambaiti, Elisa, additional, Moggia, Elisabetta, additional, Coetzee, Elmi, additional, Chisari, Emanuele, additional, D'Errico, Emanuele, additional, Ciofic, Emilia, additional, Peña, Emilio, additional, Kurt, Emine, additional, Balık, Emre, additional, Gunay, Emre, additional, Sivrikoz, Emre, additional, Andolfi, Enrico, additional, Araimo, Enrico, additional, Lucci, Enrico, additional, Opocher, Enrico, additional, Pinotti, Enrico, additional, Rubino, Enrico, additional, Reyhan, Enver, additional, Mazzotta, Erica, additional, Navarro, Ernesto Barzola, additional, El-Helou, Etienne, additional, Licardie-Bolaños, Eugenio, additional, Porto, Eva Iglesias, additional, Contreras, Evelyn, additional, Boerma, Evert-Jan, additional, Cianchi, Fabio, additional, Marino, Fabio, additional, Uggeri, Fabio, additional, Han, Fanghai, additional, Calculli, Federica, additional, Falaschi, Federica, additional, Ghignone, Federico, additional, Perrone, Federico, additional, Borghi, Felice, additional, García, Felipe, additional, Agresta, Ferdinando, additional, Cananzi, Ferdinando Carlo Maria, additional, Mendoza-Moreno, Fernando, additional, Cengiz, Fevzi, additional, Almeida, Filipe Macedo, additional, Baracchi, Filippo, additional, Carannante, Filippo, additional, La Torre, Filippo, additional, Fernandes, Flavio, additional, Friedmacher, Florian, additional, Grama, Florin, additional, Carissimi, Francesca, additional, Pecchini, Francesca, additional, Bianco, Francesco, additional, Colombo, Francesco, additional, Ferrara, Francesco, additional, Litta, Francesco, additional, Carrano, Francesco Maria, additional, Martignoni, Francesco, additional, Tasselli, Francesco Menegon, additional, Milone, Francesco, additional, Pata, Francesco, additional, Sammartino, Francesco, additional, Zambianchi, Francesco, additional, Barragan, Francisco, additional, Herrero, Francisco, additional, Schlottmann, Francisco, additional, Den Boer, Frank C, additional, Pfeffer, Frank, additional, Fujita, Fumihiko, additional, Navarra, G, additional, Herrera-Almario, Gabriel, additional, Pozzo, Gabriele, additional, Capolupo, Gabriella Teresa, additional, Van Ramshorst, Gabrielle H, additional, Liscia, Gadiel, additional, Gallo, Gaetano, additional, Asawa, Ganesh, additional, Wang, Gaoxiang, additional, Raiyani, Gaurang, additional, Beets, Geerard, additional, Naval, Gemma Sugrañes, additional, Jin, Genfeng, additional, Chang, George J, additional, Saakian, George, additional, Kahane, Gerardo, additional, Borroni, Giacomo, additional, Lo Secco, Giacomo, additional, Baiocchi, Gian Luca, additional, Baronio, Gianluca, additional, Pagano, Gianluca, additional, Pattacini, Gianmaria Casoni, additional, Lisi, Giorgio, additional, Milito, Giovanni, additional, Sinibaldi, Giovanni, additional, Serrao, Giuditta, additional, Bagaglini, Giulia, additional, Sarro, Giuliano, additional, Brisinda, Giuseppe, additional, Candilio, Giuseppe, additional, Mangiameli, Giuseppe, additional, Giuliani, Giuseppe, additional, Martin-Martin, Gonzalo Pablo, additional, Bodzin, Gordon, additional, Leon, Graat, additional, Mackay, Graham, additional, Vasil, Granila, additional, Palmisano, Graziano, additional, Giovanna, Grella Maria, additional, Fernández, Guadalupe Campos, additional, Steingel, Guillermo Berrones, additional, Zhang, Guoyun, additional, Choi, Gyu Seog, additional, Chen, Haipeng, additional, Hirose, Hajime, additional, Kayano, Hajime, additional, Ulgur, Hanife Seyda, additional, Impellizzeri, Harmony, additional, Ariola, Hasani, additional, Liu, Heli, additional, Medina, Heriberto, additional, Miyauchi, Hideaki, additional, Takahashi, Hidekazu, additional, Hayashi, Hideki, additional, Ishikawa, Hideki, additional, Ishida, Hideyuki, additional, De Vries, Hilbert, additional, Ulman, Hilmican, additional, Kon, Hirofumi, additional, Ota, Hirofumi, additional, Akamatsu, Hiroki, additional, Tamagawa, Hiroshi, additional, Shoji, Hirotaka, additional, Egi, Hiroyuki, additional, Matsubara, Hisahiro, additional, Miki, Hisanori, additional, Elfeki, Hossam, additional, Lin, Hung-Hsin, additional, Giani, Iacopo, additional, Caravaca-García, Iban, additional, Takemasa, Ichiro, additional, Angriman, Imerio, additional, Negoi, Ionut, additional, Volkova, Irina, additional, Russo, Iris, additional, Kronberger, Irmgard E, additional, Shageev, Iskander, additional, Aydin, Ishak, additional, Mora Guzmán, Ismael, additional, Novak, Ivana, additional, Giuliano, Izzo, additional, Rachmuth, Jacob, additional, Ngu, James Chi-Yong, additional, Glasbey, James, additional, Stoot, Jan, additional, Žatecky, Jan, additional, Melenhorst, Jarno, additional, Van Der Wal, J B C, additional, Leijtens, Jeroen, additional, Bogach, Jessica, additional, Elliott, Jessie, additional, De Wilt, J H W, additional, Han, Jiagang, additional, Cui, Jian, additional, Liu, Jiaqi, additional, Khan, Jim, additional, Wirawan, Jimmy Panji, additional, Zhang, Jinji, additional, Manyari, Joel Davis Osorio, additional, Doerner, Johannes, additional, Bock, Jonathan, additional, Konsten, Joop, additional, Castro, Jorge Mario, additional, Grobas, Jorge Pérez, additional, Pereira, José, additional, Juloski, Jovan, additional, Laina, Juan Luis Blas, additional, Solórzano, Juan José, additional, López, Juan Ramón Gómez, additional, Li, Jun, additional, Watanabe, Jun, additional, Kwak, Jung-Myun, additional, Hasegawa, Junichi, additional, Hiro, Junichiro, additional, Sergey, K, additional, Zhang, Kai, additional, Nagahori, Kaoru, additional, Martinez, Karla, additional, Tokuhara, Katsuji, additional, Danno, Katsuki, additional, Uehara, Kay, additional, Yoshimatsu, Kazuhiko, additional, Ehara, Kazuhisa, additional, Ueda, Kazuki, additional, Suda, Kazuyoshi, additional, Yamamoto, Kazuyuki, additional, Ishimaru, Kei, additional, Kimura, Kei, additional, Hirata, Keiji, additional, Deen, Kemal, additional, Imaizumi, Ken, additional, Yamada, Jenji, additional, Tanakura, Kenta, additional, Rida, Khaled, additional, Sugimoto, Kiichi, additional, Kotaro, Kitani, additional, Shi, Kiwisure, additional, Okabayashi, Koji, additional, Hida, Koya, additional, Kataoka, Kozo, additional, Hongo, Kumiko, additional, Xia, Kunkun, additional, Tseng, Larissa, additional, Reime, Lars, additional, Lorenzon, Laura, additional, Ruano, Laura Muiños, additional, Zhou, Lei, additional, De Nes, Lindsey, additional, Brandariz, Lorena, additional, Morini, Lorenzo, additional, Petagna, Lorenzo, additional, Ripamonti, Lorenzo, additional, Martinez, Lourdes Hernandez, additional, Pio, Luca, additional, Sacco, Luca, additional, Carvalho, Lucia, additional, Zorcolo, Luigi, additional, Pérez-Sánchez, Luis Eduardo, additional, Esparza, Luis Humberto Reyes, additional, Aguilar, Luis Tallon, additional, Garner, Madeleine, additional, Sugimoto, Maki, additional, Nagashima, Makoto, additional, Shiozawa, Manabu, additional, Simone, Manfredelli, additional, Ferrer-Marquez, Manuel, additional, Carvalho, Marcia, additional, Alifano, Marco, additional, Arganini, Marco, additional, Calussi, Marco, additional, Catarci, Marco, additional, Allaix, Marco Ettore, additional, Forlin, Marco, additional, Milone, Marco, additional, Paci, Marco, additional, Fodor, Margot, additional, Antipova, Maria, additional, Martos, Maria Beltran, additional, Giuffrida, Maria Carmela, additional, Tabernilla, María Diez, additional, Quirós, María José Alcaide, additional, Lemma, Maria, additional, Correo, Maria Luisa Reyes Diaz, additional, Małowiecka, Maria, additional, Bellomo, Maria Paola, additional, Fernandez, Maria Ramos, additional, Socias, María, additional, Rizk, Mariam, additional, Aurora, Mariani, additional, Antolinez, Mariano Alvarez, additional, Ninkovic, Marijana, additional, Giuffrida, Mario, additional, De Roos, Marnix A J, additional, Lara, Marta Cañón, additional, Agustin, Marta Climent, additional, Cuadrado, Marta, additional, Pascual, Marta, additional, Lemmerer, Martina, additional, Carlos, R, additional, Okamoto, Masa, additional, Miyo, Masaaki, additional, Inomata, Masafumi, additional, Ikenaga, Masakazu, additional, Tsujie, Masaki, additional, Yasuno, Masamichi, additional, Kotake, Masanori, additional, Sato, Masanori, additional, Yasui, Masayoshi, additional, Lavazza, Matteo, additional, Rottoli, Matteo, additional, Zuin, Matteo, additional, Zuluaga, Mauricio, additional, Cervellera, Maurizio, additional, Cesari, Maurizio, additional, Zizzo, Maurizio, additional, Garino, Mauro, additional, Ghirardi, Mauro, additional, Montuori, Mauro, additional, Podda, Mauro, additional, Santarelli, Mauro, additional, Koc, Mehmet Ali, additional, Baini, Melissa, additional, Campanelli, Michela, additional, Manigrasso, Michele, additional, Zuolo, Michele, additional, Cunha, Miguel F, additional, Misca, Mihaela, additional, Slavchev, Mihail, additional, Danilov, Mikhail, additional, Shigaev, Mikhail, additional, Martens, Milou, additional, Kobayashi, Minako, additional, Ren, Mingyang, additional, Ishizuka, Mitsuru, additional, Hassan, Mohammed Mustafa, additional, Siblini, Mohamad, additional, Sahloul, Mohamed, additional, Keramati, Mohammad Reza, additional, Karunakaran, Monish, additional, Markel, Moritz, additional, Majeed, Mudassar, additional, Younis, Muhammad Umar, additional, Akin, Muhammed Ikbal, additional, Laraibe, Munazza, additional, Derebey, Murat, additional, Kendirci, Murat, additional, Fukunaga, Mutsumi, additional, Matsubara, Nagahide, additional, Ordaz, Narce Eunice Cruz, additional, Samalavicius, Narimantas Evaldas, additional, Keeratibharat, Nattawut, additional, Angelis, Nicola, additional, Gica, Nicolae, additional, Mariani, Nicoló Maria, additional, Ramino, Nicolò, additional, Falco, Nicolò, additional, Smart, Neil, additional, De Korte, Niels, additional, Kok, Niels F M, additional, Jamieson, Nigel B, additional, Aberyasev, Nikolay, additional, Bruklich, Nikolay, additional, Ichikawa, Nobuki, additional, Miyoshi, Norikatsu, additional, De Palma, Norma, additional, Figueiredo, Nuno, additional, Torrecilla, Nuria Ortega, additional, Dybov, Oleg G, additional, Yudin, Oleg, additional, Crepin, Ollende, additional, Gomez, Oscar, additional, Sert, Ozlem Zeliha, additional, Lominchar, Pablo Lozano, additional, Menéndez, Pablo, additional, De Nardi, Paola, additional, Tejedor, Patricia, additional, Jordan, Patrick, additional, Tan, Patrick, additional, Marsanic, Patrizia, additional, Natalya, Pechnikova, additional, Baños, Pedro Parra, additional, Rebasa, Pere, additional, Neary, Peter M, additional, Tanis, Pieter, additional, Giustacchini, Piero, additional, Anoldo, Pietro, additional, Concejo, Pilar, additional, Cao, Pin, additional, Chandrasinghe, Pramodh, additional, Abeyratne, Prasad, additional, Wang, Quan, additional, Klicks, R J, additional, Riquelme, Rafael Ferrer, additional, De Luca, Raffaele, additional, Galli, Raffaele, additional, Gianesini, Raffaele, additional, Moorjani, Rajesh Gianchandani, additional, Deshpande, Rajkiran K, additional, Gorter, Ramon, additional, Ledesma, Raquel Leon, additional, Ruslan, Rategov, additional, Chhabra, Raunaq, additional, Talreja-Pelaez, Reena, additional, Suzuki, Rei, additional, Balestri, Riccardo, additional, Rosati, Riccardo, additional, Kiblawi, Rim, additional, Martins, Rita, additional, Angelico, Roberta, additional, Tutino, Roberta, additional, Persiani, Roberto, additional, Pollastri, Roberto, additional, López, Rocío González, additional, Perez, Rodrigo Oliva, additional, Hompes, Roel, additional, Lukanin, Roman, additional, Roser Termes Serra, R, additional, Brunaccino, Rossella, additional, Nakanishi, Ryota, additional, Stefan, Samuel, additional, Hernández, Sandra Paola Sánchez, additional, Di Carlo, Sara, additional, Ingallinella, Sara, additional, Domoto, Satoru, additional, Ikeda, Satoshi, additional, Mikalauskas, Saulius, additional, Kim, Seon Hahn, additional, Mantova, Serena, additional, Barbuta, Severius, additional, Li, Shaotang, additional, Yamaguchi, Shigeki, additional, Yamagishi, Shigeru, additional, Homma, Shigenori, additional, Tsujinaka, Shingo, additional, Yoshioka, Shinichi, additional, Mori, Shinichiro, additional, Tewari, Shirish, additional, Rayman, Shlomi, additional, Horiuchi, Sho, additional, Matoba, Shuichiro, additional, Morita, Shunji, additional, Yaman, Sibel, additional, Vigna, Silvia, additional, Testa, Silvio, additional, Ng, Simon, additional, Deidda, Simona, additional, Cicconi, Simone, additional, Di Maria, Simone, additional, Sibio, Simone, additional, Ersoz, Siyar, additional, Pejkova, Sofija, additional, Altarifi, Soliman, additional, He, Songbing, additional, Malakorn, Songphol, additional, Meindert, Sosef, additional, Sumikawa, Sosuke, additional, Parmar, Stavan, additional, Uranitsch, Stefan, additional, D'ugo, Stefano, additional, Giuliani, Stefano, additional, Breukink, Stéphanie, additional, Lee, Suk-Hwan, additional, Hata, Taishi, additional, Ishikawa, Takahisa, additional, Akiyoshi, Takashi, additional, Azuma, Takashi, additional, Kobatake, Takaya, additional, Fukuzaki, Takayuki, additional, Aiyama, Takeshi, additional, Yamada, Takeshi, additional, Garmanova, Tatiana, additional, Gómez-Sánchez, Tatiana, additional, Yamaguchi, Tatsuro, additional, Flores, Teresa De Jesús, additional, Usub, Teruyuki, additional, Tsuruma, Tetsuhiro, additional, Shimizu, Tetsuichiro, additional, Hristov, Tihomir Georgiev, additional, Van Loon, Ting, additional, Funakoshi, Tohru, additional, Manzia, Tommaso Maria, additional, Kiyomatsu, Tomomichi, additional, Katayama, Tomonari, additional, Kazuhito, Uemura, additional, Elmore, Ugo, additional, Grossi, Ugo, additional, Truchalev, Vadim A, additional, Rodríguez, Valentina Sosa, additional, Testa, Valentina, additional, Tonini, Valeria, additional, Celentano, Valerio, additional, Bettencourt, Vanessa, additional, Mammadov, Vasif, additional, Leyva, Verónica Alejandra Galue, additional, Mariscal, Veronica Georgina Ortega, additional, Seid, Victor Edmond, additional, Klemann, Victor, additional, Turrado-Rodriguez, Víctor, additional, Papagni, Vincenzo, additional, Vento, Vincenzo, additional, Frering, Vincent, additional, Vigorita, Vincenzo, additional, Petrove, Vitaliva V, additional, Lyadov, Vladimir, additional, Fu, Wei, additional, Mi, Wei, additional, Jeong, Woon Kyung, additional, Leclercq, Wouter K G, additional, De Sousa, Xavier, additional, Zhao, Xing, additional, Li, Xinxiang, additional, Wang, Xinxin, additional, Yang, Xuanhua, additional, Zhang, Xuelei, additional, Dong, Yan, additional, Erushevich, Yana, additional, Takii, Yasumasa, additional, Sumi, Yasuo, additional, Loli, Yeray Trujillo, additional, Yifat, Yosef Lishtzinsky, additional, Shimada, Yoshifumi, additional, Nabeya, Yoshihiro, additional, Ide, Yoshihito, additional, Wu, Yuan, additional, Tsukada, Yuichiro, additional, Miyamoto, Yuji, additional, Toiyama, Yuji, additional, Fujie, Yujiro, additional, Kaneko, Yuka, additional, Mokutani, Yukako, additional, Fujii, Yuki, additional, Kanemitsu, Yukihide, additional, Medkova, Yulia, additional, Chen, Yulong, additional, Ruiz, Yurema Gonzalez, additional, Kinugasa, Yusuke, additional, Sow, Zacaria, additional, Razzaq, Zeeshan, additional, Wang, Zejun, additional, Liu, Zheng, additional, Han, Zhenguo, additional, Tai, Zhihui, additional, Lai, Zhiyong, additional, Ng, Zi Qin, additional, and Dambrauskas, Zilvinas, additional

Published
2020
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59. Impact of asymptomatic COVID-19 patients in global surgical practice during the COVID-19 pandemic

Author

Bellato, V, primary, Konishi, T, additional, Pellino, G, additional, An, Y, additional, Piciocchi, A, additional, Sensi, B, additional, Siragusa, L, additional, Khanna, K, additional, Pirozzi, B M, additional, Franceschilli, M, additional, Campanelli, M, additional, Efetov, S, additional, Sica, G S, additional, Kefleyesus, A, additional, Hoofwijk, A G M, additional, Eldaly, Abdullah Sami, additional, Gonzalez, Abel, additional, Jawad, Adraoui, additional, Jooma, Ahmad, additional, Hafez, Ahmed Mousa, additional, Rubio, Ainhoa Valle, additional, Landaluce-Olavarria, Aitor, additional, Wu, Aiwen, additional, Nagatsu, Akihisa, additional, Inoue, Akira, additional, Kanamoto, Akira, additional, Ouchi, Akira, additional, El-Hussuna, Alaa, additional, Vazquez-Melero, Alba, additional, Wolthuis, Albert M, additional, Peral, Alberto M, additional, Lozano, Alejandra Cruz, additional, Efremov, Aleksandr, additional, Ryasantsev, Aleksandr V, additional, Di Giorgio, Alessandra, additional, Parente, Alessandro, additional, Tamburrini, Alessandro, additional, Alò, Alessio, additional, Forero-Torres, Alexander, additional, Vahrmeijer, Alexander L, additional, Varabei, Alexander, additional, Hinojosa, Siles, additional, Balkan, Ali Zeynel Abidin, additional, Frontali, Alice, additional, Oleg, Alikin, additional, Soler-Silva, Álvaro, additional, Makni, Amin, additional, André, Ana, additional, Cabrera, Ana María García, additional, Fernández, Ana María Gonzalez, additional, Minaya-Bravo, Ana M, additional, Rodríguez-Sánchez, Ana, additional, Musina, Ana-Maria, additional, Pangeni, Anang, additional, Zolotko, Anastasia, additional, Tonoyan, Andranik, additional, Balla, Andrea, additional, Belli, Andrea, additional, Cavallaro, Andrea, additional, Chierici, Andrea, additional, Divizia, Andrea, additional, Bucci, Andrea Fares, additional, Salido, Andrea Jiménez, additional, Morini, Andrea, additional, Muratore, Andrea, additional, Vignali, Andrea, additional, Chitul, Andrei, additional, Sebastian, Diaconescu Andrei, additional, Pcolkins, Andrejs, additional, Shchegolev, Andrey, additional, Hollenbeck, Andrew, additional, Wisneski, Andrew, additional, Iossa, Angelo, additional, D'Amore, Anna, additional, Hunter, Anndrew, additional, Hesketh, Anthony J, additional, La Brocca, Andrea, additional, Spinelli, Antonino, additional, Caires, Antonio, additional, D'Alessandro, Antonio, additional, Correo, Antonio Francisco Sanchís López, additional, Macrì, Antonio, additional, Navarro-Sánchez, Antonio, additional, Pronk, Apollo, additional, Akunc, Aram, additional, Mehri, Arash, additional, Pelta, Arie, additional, Papadopoulos, Aristeidis, additional, Kechagias, Aristotelis, additional, Rashid, Arshad, additional, Ramazanov, Artur, additional, Chandio, Ashfaq, additional, Kohyama, Atsushi, additional, Nishimura, Atsushi, additional, Ohkawa, Atsushi, additional, Dulskas, Audrius, additional, Jamal, Aun, additional, Mariani, Aurora, additional, Unal, Ayse Gizem, additional, Karagoz, Ayse, additional, Ozkan, Bahar Busra, additional, Salih, Barham, additional, Gülcü, Baris, additional, Pessia, Beatrice, additional, Martin-Perez, Beatriz, additional, Ielpo, Benedetto, additional, Tulelli, Berenice, additional, Yang, Bin, additional, Mhamed, Boumadani, additional, Murphy, Brenda, additional, Pirozzi, Brunella Maria, additional, Sensi, Bruno, additional, Langenhoff, Bs, additional, Belevi, Bulent, additional, Güney, Burak, additional, Ng, Caecilia, additional, Rueda, Camilo, additional, Roxburgh, Campbell S, additional, Feo, Carlo V, additional, Ferrari, Carlo, additional, Gazia, Carlo, additional, Pratesi, Carlo, additional, Ratto, Carlo, additional, Santacruz, Carlos Cerdán, additional, Arroyave, Carlos Rodolfo Martinez, additional, Macias, Carlos, additional, Fernandez, Carlota Garcia, additional, Fernandez, Carmen Cagigas, additional, Curtis-Martinez, Carolina, additional, Fortmann, Caroline, additional, Kim, Caroline, additional, Galeano, Catalina Uribe, additional, Barroso, Catarina, additional, Baldi, Caterina, additional, Foppa, Caterina, additional, Formisano, Cesare, additional, Li, Changzai, additional, Ding, Chao, additional, Wang, Chenyu, additional, Iacusso, Chiara, additional, Yang, Chongwei, additional, Pizzera, Christian, additional, Skias, Christoph, additional, Chouliras, Christos, additional, Liakos, Christos, additional, Matsuda, Chu, additional, Wu, Chun-yi, additional, Ozaslan, Cihangir, additional, Tanda, Cinzia, additional, Tommaso, Cipolat Mis, additional, Dagorno, Claire, additional, Ramos, Claudia Patricia Arellano, additional, Arcudi, Claudio, additional, Coco, Claudio, additional, Morales, Cleotilde Mateo, additional, Ali, Mujahid Zulfiqar, additional, Lozano, Coral Cózar, additional, Sala, Corrado, additional, Leo, Cosimo Alex, additional, Scarpa, Cosimo Riccardo, additional, Ferro, Cristian Varela, additional, Fernandez, Cristina Mosquera, additional, Morales-Garcia, D, additional, Nakano, Daisuke, additional, Cristian, Daniel, additional, Hechtl, Daniel, additional, Cánovas, Daniel Triguero, additional, Calabrese, Daniela, additional, Rega, Daniela, additional, Ferraro, Daniele, additional, Morezzi, Daniele, additional, Sommacale, Daniele, additional, Brogden, Danielle, additional, Miskovic, Danilo, additional, Merlini, David, additional, Pertile, Davide, additional, Coniglio, Denise, additional, Zhu, Dexiang, additional, Wu, Dianwen, additional, Coletta, Diego, additional, Rubio, Diego Ramos, additional, Sasia, Diego, additional, Fillipov, Dmitry, additional, Russiello, Domenico, additional, Dardanov, Dragomir, additional, Consten, E C J, additional, Smolskas, Edgaras, additional, Muttillo, Edoardo Maria, additional, Jones, Edward, additional, Sunami, Eiji, additional, Etienne, El-Helou, additional, Chalkiadaki, Elena, additional, Giacomelli, Elena, additional, Karbovnichaya, Elena, additional, Ruiz-Úcar, Elena, additional, Guaitoli, Eleonora, additional, Samadov, Elgun, additional, Jovine, Elio, additional, Treppiedi, Elio, additional, Vaterlini, Elisa Maria, additional, Zambaiti, Elisa, additional, Moggia, Elisabetta, additional, Coetzee, Elmi, additional, Chisari, Emanuele, additional, D'Errico, Emanuele, additional, Ciofic, Emilia, additional, Peña, Emilio, additional, Kurt, Emine, additional, Balık, Emre, additional, Gunay, Emre, additional, Sivrikoz, Emre, additional, Andolfi, Enrico, additional, Araimo, Enrico, additional, Lucci, Enrico, additional, Opocher, Enrico, additional, Pinotti, Enrico, additional, Rubino, Enrico, additional, Reyhan, Enver, additional, Mazzotta, Erica, additional, Navarro, Ernesto Barzola, additional, El-Helou, Etienne, additional, Licardie-Bolaños, Eugenio, additional, Porto, Eva Iglesias, additional, Contreras, Evelyn, additional, Boerma, Evert-Jan, additional, Cianchi, Fabio, additional, Marino, Fabio, additional, Uggeri, Fabio, additional, Han, Fanghai, additional, Calculli, Federica, additional, Falaschi, Federica, additional, Ghignone, Federico, additional, Perrone, Federico, additional, Borghi, Felice, additional, García, Felipe, additional, Agresta, Ferdinando, additional, Cananzi, Ferdinando Carlo Maria, additional, Mendoza-Moreno, Fernando, additional, Cengiz, Fevzi, additional, Almeida, Filipe Macedo, additional, Baracchi, Filippo, additional, Carannante, Filippo, additional, La Torre, Filippo, additional, Fernandes, Flavio, additional, Friedmacher, Florian, additional, Grama, Florin, additional, Carissimi, Francesca, additional, Pecchini, Francesca, additional, Bianco, Francesco, additional, Colombo, Francesco, additional, Ferrara, Francesco, additional, Litta, Francesco, additional, Carrano, Francesco Maria, additional, Martignoni, Francesco, additional, Tasselli, Francesco Menegon, additional, Milone, Francesco, additional, Pata, Francesco, additional, Sammartino, Francesco, additional, Zambianchi, Francesco, additional, Barragan, Francisco, additional, Herrero, Francisco, additional, Schlottmann, Francisco, additional, Den Boer, Frank C, additional, Pfeffer, Frank, additional, Fujita, Fumihiko, additional, Navarra, G, additional, Herrera-Almario, Gabriel, additional, Pozzo, Gabriele, additional, Capolupo, Gabriella Teresa, additional, Van Ramshorst, Gabrielle H, additional, Liscia, Gadiel, additional, Gallo, Gaetano, additional, Asawa, Ganesh, additional, Wang, Gaoxiang, additional, Raiyani, Gaurang, additional, Beets, Geerard, additional, Naval, Gemma Sugrañes, additional, Jin, Genfeng, additional, Chang, George J, additional, Saakian, George, additional, Kahane, Gerardo, additional, Borroni, Giacomo, additional, Lo Secco, Giacomo, additional, Baiocchi, Gian Luca, additional, Baronio, Gianluca, additional, Pagano, Gianluca, additional, Pattacini, Gianmaria Casoni, additional, Lisi, Giorgio, additional, Milito, Giovanni, additional, Sinibaldi, Giovanni, additional, Serrao, Giuditta, additional, Bagaglini, Giulia, additional, Sarro, Giuliano, additional, Brisinda, Giuseppe, additional, Candilio, Giuseppe, additional, Mangiameli, Giuseppe, additional, Giuliani, Giuseppe, additional, Martin-Martin, Gonzalo Pablo, additional, Bodzin, Gordon, additional, Leon, Graat, additional, Mackay, Graham, additional, Vasil, Granila, additional, Palmisano, Graziano, additional, Giovanna, Grella Maria, additional, Fernández, Guadalupe Campos, additional, Steingel, Guillermo Berrones, additional, Zhang, Guoyun, additional, Choi, Gyu Seog, additional, Chen, Haipeng, additional, Hirose, Hajime, additional, Kayano, Hajime, additional, Ulgur, Hanife Seyda, additional, Impellizzeri, Harmony, additional, Ariola, Hasani, additional, Liu, Heli, additional, Medina, Heriberto, additional, Miyauchi, Hideaki, additional, Takahashi, Hidekazu, additional, Hayashi, Hideki, additional, Ishikawa, Hideki, additional, Ishida, Hideyuki, additional, De Vries, Hilbert, additional, Ulman, Hilmican, additional, Kon, Hirofumi, additional, Ota, Hirofumi, additional, Akamatsu, Hiroki, additional, Tamagawa, Hiroshi, additional, Shoji, Hirotaka, additional, Egi, Hiroyuki, additional, Matsubara, Hisahiro, additional, Miki, Hisanori, additional, Elfeki, Hossam, additional, Lin, Hung-Hsin, additional, Giani, Iacopo, additional, Caravaca-García, Iban, additional, Takemasa, Ichiro, additional, Angriman, Imerio, additional, Negoi, Ionut, additional, Volkova, Irina, additional, Russo, Iris, additional, Kronberger, Irmgard E, additional, Shageev, Iskander, additional, Aydin, Ishak, additional, Mora Guzmán, Ismael, additional, Novak, Ivana, additional, Giuliano, Izzo, additional, Rachmuth, Jacob, additional, Ngu, James Chi-Yong, additional, Glasbey, James, additional, Stoot, Jan, additional, Žatecky, Jan, additional, Melenhorst, Jarno, additional, Van Der Wal, J B C, additional, Leijtens, Jeroen, additional, Bogach, Jessica, additional, Elliott, Jessie, additional, De Wilt, J H W, additional, Han, Jiagang, additional, Cui, Jian, additional, Liu, Jiaqi, additional, Khan, Jim, additional, Wirawan, Jimmy Panji, additional, Zhang, Jinji, additional, Manyari, Joel Davis Osorio, additional, Doerner, Johannes, additional, Bock, Jonathan, additional, Konsten, Joop, additional, Castro, Jorge Mario, additional, Grobas, Jorge Pérez, additional, Pereira, José, additional, Juloski, Jovan, additional, Laina, Juan Luis Blas, additional, Solórzano, Juan José, additional, López, Juan Ramón Gómez, additional, Li, Jun, additional, Watanabe, Jun, additional, Kwak, Jung-Myun, additional, Hasegawa, Junichi, additional, Hiro, Junichiro, additional, Sergey, K, additional, Zhang, Kai, additional, Nagahori, Kaoru, additional, Martinez, Karla, additional, Tokuhara, Katsuji, additional, Danno, Katsuki, additional, Uehara, Kay, additional, Yoshimatsu, Kazuhiko, additional, Ehara, Kazuhisa, additional, Ueda, Kazuki, additional, Suda, Kazuyoshi, additional, Yamamoto, Kazuyuki, additional, Ishimaru, Kei, additional, Kimura, Kei, additional, Hirata, Keiji, additional, Deen, Kemal, additional, Imaizumi, Ken, additional, Yamada, Jenji, additional, Tanakura, Kenta, additional, Rida, Khaled, additional, Sugimoto, Kiichi, additional, Kotaro, Kitani, additional, Shi, Kiwisure, additional, Okabayashi, Koji, additional, Hida, Koya, additional, Kataoka, Kozo, additional, Hongo, Kumiko, additional, Xia, Kunkun, additional, Tseng, Larissa, additional, Reime, Lars, additional, Lorenzon, Laura, additional, Ruano, Laura Muiños, additional, Siragusa, Leandro, additional, Zhou, Lei, additional, De Nes, Lindsey, additional, Brandariz, Lorena, additional, Morini, Lorenzo, additional, Petagna, Lorenzo, additional, Ripamonti, Lorenzo, additional, Martinez, Lourdes Hernandez, additional, Pio, Luca, additional, Sacco, Luca, additional, Carvalho, Lucia, additional, Zorcolo, Luigi, additional, Pérez-Sánchez, Luis Eduardo, additional, Esparza, Luis Humberto Reyes, additional, Aguilar, Luis Tallon, additional, Garner, Madeleine, additional, Sugimoto, Maki, additional, Nagashima, Makoto, additional, Shiozawa, Manabu, additional, Simone, Manfredelli, additional, Ferrer-Marquez, Manuel, additional, Carvalho, Marcia, additional, Alifano, Marco, additional, Arganini, Marco, additional, Calussi, Marco, additional, Catarci, Marco, additional, Allaix, Marco Ettore, additional, Forlin, Marco, additional, Milone, Marco, additional, Paci, Marco, additional, Franceschilli, Marzia, additional, Fodor, Margot, additional, Antipova, Maria, additional, Martos, Maria Beltran, additional, Giuffrida, Maria Carmela, additional, Tabernilla, María Diez, additional, Quirós, María José Alcaide, additional, Lemma, Maria, additional, Correo, Maria Luisa Reyes Diaz, additional, Małowiecka, Maria, additional, Bellomo, Maria Paola, additional, Fernandez, Maria Ramos, additional, Socias, María, additional, Rizk, Mariam, additional, Aurora, Mariani, additional, Antolinez, Mariano Alvarez, additional, Ninkovic, Marijana, additional, Giuffrida, Mario, additional, De Roos, Marnix A J, additional, Lara, Marta Cañón, additional, Agustin, Marta Climent, additional, Cuadrado, Marta, additional, Pascual, Marta, additional, Lemmerer, Martina, additional, Carlos, R, additional, Okamoto, Masa, additional, Miyo, Masaaki, additional, Inomata, Masafumi, additional, Ikenaga, Masakazu, additional, Tsujie, Masaki, additional, Yasuno, Masamichi, additional, Kotake, Masanori, additional, Sato, Masanori, additional, Yasui, Masayoshi, additional, Lavazza, Matteo, additional, Rottoli, Matteo, additional, Zuin, Matteo, additional, Zuluaga, Mauricio, additional, Cervellera, Maurizio, additional, Cesari, Maurizio, additional, Zizzo, Maurizio, additional, Garino, Mauro, additional, Ghirardi, Mauro, additional, Montuori, Mauro, additional, Podda, Mauro, additional, Santarelli, Mauro, additional, Koc, Mehmet Ali, additional, Baini, Melissa, additional, Campanelli, Michela, additional, Manigrasso, Michele, additional, Zuolo, Michele, additional, Cunha, Miguel F, additional, Misca, Mihaela, additional, Slavchev, Mihail, additional, Danilov, Mikhail, additional, Shigaev, Mikhail, additional, Martens, Milou, additional, Kobayashi, Minako, additional, Ren, Mingyang, additional, Ishizuka, Mitsuru, additional, Hassan, Mohammed Mustafa, additional, Siblini, Mohamad, additional, Sahloul, Mohamed, additional, Keramati, Mohammad Reza, additional, Karunakaran, Monish, additional, Markel, Moritz, additional, Majeed, Mudassar, additional, Younis, Muhammad Umar, additional, Akin, Muhammed Ikbal, additional, Laraibe, Munazza, additional, Derebey, Murat, additional, Kendirci, Murat, additional, Fukunaga, Mutsumi, additional, Matsubara, Nagahide, additional, Ordaz, Narce Eunice Cruz, additional, Samalavicius, Narimantas Evaldas, additional, Keeratibharat, Nattawut, additional, Angelis, Nicola, additional, Gica, Nicolae, additional, Mariani, Nicoló Maria, additional, Ramino, Nicolò, additional, Falco, Nicolò, additional, Smart, Neil, additional, De Korte, Niels, additional, Kok, Niels F M, additional, Jamieson, Nigel B, additional, Aberyasev, Nikolay, additional, Bruklich, Nikolay, additional, Ichikawa, Nobuki, additional, Miyoshi, Norikatsu, additional, De Palma, Norma, additional, Figueiredo, Nuno, additional, Torrecilla, Nuria Ortega, additional, Dybov, Oleg G, additional, Yudin, Oleg, additional, Crepin, Ollende, additional, Gomez, Oscar, additional, Sert, Ozlem Zeliha, additional, Lominchar, Pablo Lozano, additional, Menéndez, Pablo, additional, De Nardi, Paola, additional, Tejedor, Patricia, additional, Jordan, Patrick, additional, Tan, Patrick, additional, Marsanic, Patrizia, additional, Natalya, Pechnikova, additional, Baños, Pedro Parra, additional, Rebasa, Pere, additional, Neary, Peter M, additional, Tanis, Pieter, additional, Giustacchini, Piero, additional, Anoldo, Pietro, additional, Concejo, Pilar, additional, Cao, Pin, additional, Chandrasinghe, Pramodh, additional, Abeyratne, Prasad, additional, Wang, Quan, additional, Klicks, R J, additional, Riquelme, Rafael Ferrer, additional, De Luca, Raffaele, additional, Galli, Raffaele, additional, Gianesini, Raffaele, additional, Moorjani, Rajesh Gianchandani, additional, Deshpande, Rajkiran K, additional, Gorter, Ramon, additional, Ledesma, Raquel Leon, additional, Ruslan, Rategov, additional, Chhabra, Raunaq, additional, Talreja-Pelaez, Reena, additional, Suzuki, Rei, additional, Balestri, Riccardo, additional, Rosati, Riccardo, additional, Kiblawi, Rim, additional, Martins, Rita, additional, Angelico, Roberta, additional, Tutino, Roberta, additional, Persiani, Roberto, additional, Pollastri, Roberto, additional, López, Rocío González, additional, Perez, Rodrigo Oliva, additional, Hompes, Roel, additional, Lukanin, Roman, additional, Roser Termes Serra, R, additional, Brunaccino, Rossella, additional, Nakanishi, Ryota, additional, Stefan, Samuel, additional, Hernández, Sandra Paola Sánchez, additional, Di Carlo, Sara, additional, Ingallinella, Sara, additional, Domoto, Satoru, additional, Ikeda, Satoshi, additional, Mikalauskas, Saulius, additional, Kim, Seon Hahn, additional, Mantova, Serena, additional, Barbuta, Severius, additional, Li, Shaotang, additional, Yamaguchi, Shigeki, additional, Yamagishi, Shigeru, additional, Homma, Shigenori, additional, Tsujinaka, Shingo, additional, Yoshioka, Shinichi, additional, Mori, Shinichiro, additional, Tewari, Shirish, additional, Rayman, Shlomi, additional, Horiuchi, Sho, additional, Matoba, Shuichiro, additional, Morita, Shunji, additional, Yaman, Sibel, additional, Vigna, Silvia, additional, Testa, Silvio, additional, Ng, Simon, additional, Deidda, Simona, additional, Cicconi, Simone, additional, Di Maria, Simone, additional, Sibio, Simone, additional, Ersoz, Siyar, additional, Pejkova, Sofija, additional, Altarifi, Soliman, additional, He, Songbing, additional, Malakorn, Songphol, additional, Meindert, Sosef, additional, Sumikawa, Sosuke, additional, Parmar, Stavan, additional, Uranitsch, Stefan, additional, D'ugo, Stefano, additional, Giuliani, Stefano, additional, Breukink, Stéphanie, additional, Lee, Suk-Hwan, additional, Hata, Taishi, additional, Ishikawa, Takahisa, additional, Akiyoshi, Takashi, additional, Azuma, Takashi, additional, Kobatake, Takaya, additional, Fukuzaki, Takayuki, additional, Aiyama, Takeshi, additional, Yamada, Takeshi, additional, Garmanova, Tatiana, additional, Gómez-Sánchez, Tatiana, additional, Yamaguchi, Tatsuro, additional, Flores, Teresa De Jesús, additional, Usub, Teruyuki, additional, Tsuruma, Tetsuhiro, additional, Shimizu, Tetsuichiro, additional, Hristov, Tihomir Georgiev, additional, Van Loon, Ting, additional, Funakoshi, Tohru, additional, Manzia, Tommaso Maria, additional, Kiyomatsu, Tomomichi, additional, Katayama, Tomonari, additional, Kazuhito, Uemura, additional, Elmore, Ugo, additional, Grossi, Ugo, additional, Truchalev, Vadim A, additional, Rodríguez, Valentina Sosa, additional, Testa, Valentina, additional, Tonini, Valeria, additional, Celentano, Valerio, additional, Bettencourt, Vanessa, additional, Mammadov, Vasif, additional, Leyva, Verónica Alejandra Galue, additional, Mariscal, Veronica Georgina Ortega, additional, Seid, Victor Edmond, additional, Klemann, Victor, additional, Turrado-Rodriguez, Víctor, additional, Papagni, Vincenzo, additional, Vento, Vincenzo, additional, Frering, Vincent, additional, Vigorita, Vincenzo, additional, Petrove, Vitaliva V, additional, Lyadov, Vladimir, additional, Fu, Wei, additional, Mi, Wei, additional, Jeong, Woon Kyung, additional, Leclercq, Wouter K G, additional, De Sousa, Xavier, additional, Zhao, Xing, additional, Li, Xinxiang, additional, Wang, Xinxin, additional, Yang, Xuanhua, additional, Zhang, Xuelei, additional, Dong, Yan, additional, Erushevich, Yana, additional, Takii, Yasumasa, additional, Sumi, Yasuo, additional, Loli, Yeray Trujillo, additional, Yifat, Yosef Lishtzinsky, additional, Shimada, Yoshifumi, additional, Nabeya, Yoshihiro, additional, Ide, Yoshihito, additional, Wu, Yuan, additional, Tsukada, Yuichiro, additional, Miyamoto, Yuji, additional, Toiyama, Yuji, additional, Fujie, Yujiro, additional, Kaneko, Yuka, additional, Mokutani, Yukako, additional, Fujii, Yuki, additional, Kanemitsu, Yukihide, additional, Medkova, Yulia, additional, Chen, Yulong, additional, Ruiz, Yurema Gonzalez, additional, Kinugasa, Yusuke, additional, Sow, Zacaria, additional, Razzaq, Zeeshan, additional, Wang, Zejun, additional, Liu, Zheng, additional, Han, Zhenguo, additional, Tai, Zhihui, additional, Lai, Zhiyong, additional, Ng, Zi Qin, additional, and Dambrauskas, Zilvinas, additional

Published
2020
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64. CCL3 and CCL20-recruited dendritic cells modified by melanoma antigen gene-1 induce anti-tumor immunity against gastric cancer ex vivo and in vivo

Author

Zhang Yanyun, Li Dechun, Wu Yugang, Wang Liang, and He Songbing

Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Abstract Background To investigate whether dendritic cell (DC) precursors, recruited by injection of chemokine ligand 3 (CCL3) and CCL20, induce anti-tumor immunity against gastric cancer induced by a DC vaccine expressing melanoma antigen gene-1 (MAGE-1) ex vivo and in vivo. Methods B6 mice were injected with CCL3 and CCL20 via the tail vein. Freshly isolated F4/80-B220-CD11c+ cells cultured with cytokines were analyzed by phenotype analysis and mixed lymphocyte reaction (MLR). For adenoviral (Ad)-mediated gene transduction, cultured F4/80-B220-CD11c+ cells were incubated with Ad-MAGE-1. Vaccination of stimulated DC induced T lymphocytes. The killing effect of these T cells against gastric carcinoma cells was assayed by MTT. INF-γ production was determined with an INF-γ ELISA kit. In the solid tumor and metastases model, DC-based vaccines were used for immunization after challenge with MFC cells. Tumor size, survival of mice, and number of pulmonary metastatic foci were used to assess the therapeutic effect of DC vaccines. Results F4/80-B220-CD11c+ cell numbers increased after CCL3 and CCL20 injection. Freshly isolated F4/80-B220-CD11c+ cells cultured with cytokines were phenotyically identical to typical DC and gained the capacity to stimulate allogeneic T cells. These DCs were transduced with Ad-MAGE-1, which were prepared for DC vaccines expressing tumor antigen. T lymphocytes stimulated by DCs transduced with Ad-MAGE-1 exhibited specific killing effects on gastric carcinoma cells and produced high levels of INF-γ ex vivo. In vivo, tumor sizes of the experimental group were much smaller than both the positive control group and the negative control groups (P < 0.05). Kaplan-Meier survival curves showed that survival of the experimental group mice was significantly longer than the control groups (P < 0.05). In addition, MAGE-1-transduced DCs were also a therapeutic benefit on an established metastatic tumor, resulting in a tremendous decrease in the number of pulmonary metastatic foci. Conclusions CCL3 and CCL20-recruited DCs modified by adenovirus-trasnsduced, tumor-associated antigen, MAGE-1, can stimulate anti-tumor immunity specific to gastric cancer ex vivo and in vivo. This system may prove to be an efficient strategy for anti-tumor immunotherapy.

Published
2010
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66. Reduced production of laminin by hepatic stellate cells contributes to impairment in oval cell response to liver injury in aged mice

Author

Qian, Liu, primary, Zhang, Hui, additional, Gu, Yuting, additional, Li, Dechun, additional, He, Songbing, additional, Wang, Hui, additional, Cheng, Yiji, additional, Yang, Wanlin, additional, Yu, Hongshuang, additional, Zhao, Xiaonan, additional, Cai, Wei, additional, Meng, Lijun, additional, Jin, Min, additional, Wang, Yanan, additional, and Zhang, Yanyun, additional

Published
2018
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68. Small interfering RNA‑mediated knockdown of fatty acid synthase attenuates the proliferation and metastasis of human gastric cancer cells via the mTOR/Gli1 signaling pathway

Author

Sun, Liang, primary, Yao, Yizhou, additional, Pan, Guofeng, additional, Zhan, Shenghua, additional, Shi, Weiqiang, additional, Lu, Ting, additional, Yuan, Jinfeng, additional, Tian, Kangjun, additional, Jiang, Linhua, additional, Song, Shiduo, additional, Zhu, Xinguo, additional, and He, Songbing, additional

Published
2018
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72. The application of a robotic single‐incision plus one‐port laparoscopic total colectomy with fascia space priority for slow‐transit constipation – A video vignette.

Author

Zhou, Qiyang, Hu, You, Chen, Xin, He, Songbing, and Zhou, Xiaojun

Subjects
COLECTOMY, CONSTIPATION, LAPAROSCOPIC surgery, MINIMALLY invasive procedures, SURGICAL site, OPERATIVE surgery
Abstract

Comparison of laparoscopic subtotal colectomy with posterior vaginal suspension and laparoscopic subtotal colectomy with transvaginal repair for patients with slow-transit constipation complicated with rectocele: a non-randomized comparative study in a single center. Slow-transit constipation (STC) is a colonic motor dysfunction that affects approximately 55% of patients with chronic constipation [[1]]. Surgical treatment for STC has dramatically developed, including total colectomy with ileorectal anastomosis (IRA), subtotal colectomy with caecorectal anastomosis, colonic exclusion, and so on [[2]]. [Extracted from the article]

Published
2023
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73. Downregulation of Enhancer of Zeste Homolog 2 (EZH2) is essential for the Induction of Autophagy and Apoptosis in Colorectal Cancer Cells

Author

Yao, Yizhou, primary, Hu, Hao, additional, Yang, Yong, additional, Zhou, Guoqiang, additional, Shang, Zengfu, additional, Yang, Xiaodong, additional, Sun, Kang, additional, Zhan, Shenghua, additional, Yu, Zhengyuan, additional, Li, Peiyao, additional, Pan, Guofeng, additional, Sun, Liang, additional, Zhu, Xinguo, additional, and He, Songbing, additional

Published
2016
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76. miRNA-101 Suppresses Epithelial-to-mesenchymal Transition by Targeting HMGA2 in Pancreatic Cancer Cells

Author

Jiang, Wenli, primary, Gu, Wen, additional, Qiu, Ronglin, additional, He, Songbing, additional, Shen, Chenglong, additional, Wu, Yaohao, additional, Zhang, Jie, additional, Zhou, Jiajia, additional, Guo, Yixing, additional, Wan, Daiwei, additional, Li, Zhixi, additional, Deng, Jiemin, additional, Zeng, Lexiang, additional, Tang, Jin, additional, Zhou, Jin, additional, Zhi, Qiaoming, additional, and Deng, Xiaogeng, additional

Published
2016
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77. miR-203 Suppresses the Proliferation and Metastasis of Hepatocellular Carcinoma by Targeting Oncogene ADAM9 and Oncogenic Long Non-coding RNA HULC

Author

Wan, Daiwei, primary, Shen, Shunli, additional, Fu, Shunjun, additional, Preston, Burnley, additional, Brandon, Coder, additional, He, Songbing, additional, Shen, Chenglong, additional, Wu, Jian, additional, Wang, Sutong, additional, Xie, Wenxuan, additional, Chen, Bin, additional, A, Liya, additional, Guo, Yixing, additional, Zheng, Dingcheng, additional, Zhi, Qiaoming, additional, and Peng, Baogang, additional

Published
2016
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80. The coexpression and clinical significance of costimulatory molecules B7-H1, B7-H3, and B7-H4 in human pancreatic cancer

Author

Chen,Yan, Sun,Jing, Zhao,Hua, Zhu,Dongming, Zhi,Qiaoming, Song,Shiduo, Zhang,Lifeng, He,Songbing, Kuang,Yuting, Zhang,Zixiang, Li,Dechun, Chen,Yan, Sun,Jing, Zhao,Hua, Zhu,Dongming, Zhi,Qiaoming, Song,Shiduo, Zhang,Lifeng, He,Songbing, Kuang,Yuting, Zhang,Zixiang, and Li,Dechun

Abstract

Yan Chen,1,* Jing Sun,2,* Hua Zhao,1 Dongming Zhu,1 Qiaoming Zhi,1 Shiduo Song,1 Lifeng Zhang,1 Songbing He,1 Yuting Kuang,1 Zixiang Zhang,1 Dechun Li1 1Department of General Surgery, First Affiliated Hospital of Soochow University, 2Department of Immunology, Soochow University, Suzhou, People's Republic of China *These authors contributed equally to this work Aim: We investigated the expression of the inhibitory costimulatory molecules B7-H1, B7-H3, and B7-H4 in human pancreatic cancer to define their clinical significance and mechanism in a tumor microenvironment. Patients and methods: Sixty-three pancreatic cancer tissues and 12 normal pancreatic tissues were examined in our research. Patients were enrolled in the study between December 2000 and August 2010. Expression levels of the B7 family of molecules and densities of tumor-infiltrating lymphocytes in the tissues were characterized with immunohistochemical assays. Results: More than 50% of the patients expressed B7-H1 and B7-H4, and nearly 100% of the patients expressed B7-H3. B7-H1 expression was correlated with tumor size, B7-H3 expression was correlated with lymph-node metastasis and differentiation grade, and B7-H4 expression was correlated with tumor size, lymph-node metastasis, and invasion depth. High B7-H4 expression was also correlated with poor survival in pancreatic cancer. We determined the value of these three B7 family molecules in the postoperative survival prognosis for patients with pancreatic cancer, and pancreatic cancer patients with less coexpression of the B7 family of molecules had a significantly higher survival rate. B7-H1 expression was found to be negatively related to the intensity of both CD3+ T cells and CD8+ T cells, and B7-H4 expression was negatively related to CD3+ T-cell infiltration intensity, but not to CD8+ T cells. Conclusion: B7-H1, B7-H3, and B7-H4 are involved in pancreatic cancer progression, and their coexpression could be a valuable prognostic indicator. Ne

Published
2014

83. Abstract 4094: Identification of co-inhibitory receptor expression on T cells from gastric cancer patients

Author

Zong, Yunhui, primary, Chu, Fuliang, additional, He, Songbing, additional, Jing, Yu, additional, Hunsucker, Sally A, additional, Yang, Tina, additional, Zhang, Ming, additional, Ye, Sisi, additional, Li, Yafen, additional, Liu, Bin, additional, Tang, Jinle, additional, Meng, Huimin, additional, An, Gangli, additional, and Yang, Lin, additional

Published
2014
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95. Variation and treatment of vessels in laparoscopic right hemicolectomy

Author

Ye, Kai, Lin, Jianan, Sun, Yafeng, Wu, Yiyang, Xu, Jianhua, and He, Songbing

Abstract

With the introduction of complete mesocolic excision (CME) and the application of laparoscopic technique, surgery for colon cancer has become more standardized and the curative effect has improved [1]. The key points in laparoscopic right hemicolectomy are high ligation of main vessels and root dissection of lymph nodes. The wide range of variations in vascular architecture and intraoperative bleeding are common causes of prolonged surgical time, wound hemorrhage, and even transfer to the opening operation. The superior mesenteric vein (SMV) is the most important anatomical landmark in CME for the right colon, and guides all the steps of lymph node dissection. The SMV appears as a pale blue bulge on laparoscopy, which enables accurate positioning. The ileocolic vessel pedicle is relatively constant and facilitates accurate positioning. The intersection of the ileocolic vessel pedicle and the SMV is the optimal starting point in laparoscopic right hemicolectomy using a medial-to-lateral approach. A sheath with an avascular plane can be reached after opening the SMV vascular sheath, which results in less bleeding and enables vascular root and thorough lymph node dissection. The first step is to manage the ileocolic vessels. The ileocolic artery (ICA) is located anterior to the ileocolic vein (ICV) for about one-third of the incidence. The ileocolic vessels are relatively long and are easy to work with. In the vast majority of cases, the ICV drains into the SMV, and into the gastrocolic trunk (GCT) in about 2.5% of cases. The reported incidence of a right colic artery (RCA) is controversial; the RCA is absent in about 50% of cases and often crosses the SMV. The right colic vein (RCV) usually drains into the GCT, but sometimes drains directly into the SMV. The middle colic vessels have great variability and a close anatomical relationship with the pancreas, duodenum, and GCT. Moreover, the transverse colon and mesentery are long, and root positioning and processing of the middle colic vein (MCV) are relatively difficult. With the SMV and pancreas as anatomic landmarks, it is more feasible to locate the blood vessels in the neck of the pancreas. The middle colic artery (MCA) originates from the superior mesenteric artery (SMA), and the distance from the inferior border of the pancreas differs slightly in the literature, but is at the most 5 cm. Identification of the MCA trunk and branches, as well as the common origin of the MCA and RCA, is of great importance for the maintaining the blood supply during surgery for primary colon cancer. The MCV mainly drains into the SMV and GCT; however, if branching variation drains into the jejunal vein, inferior mesenteric vein, or splenic vein, the effect is serious when a vessel is torn. Isolation of the GCT is the step at which bleeding will likely occur in standard right resection and is a difficult stage of the surgery. The GCT has five origins including the right gastroepiploic vein (RGV), right colic vein (RCV), accessory right colic vein (ARCV), pancreaticduodenal vein (PDV), and MCV, which can have 2, 3, or 4 branches; therefore, familiarity with variants may be helpful to avoid bleeding. Approximately 5–10% of colon cancers at the hepatic flexure have No. 6 group lymph node metastasis, and laparoscopic radical extended right hemicolectomy requires thorough dissection of No. 6 group lymph nodes and the omental arcade 10 cm from the pylorus. The inferior arteriovenous vessels are a common source of bleeding, and the RGV can serve as a clue to finding the artery. The core area of laparoscopic radical extended right hemicolectomy includes the pancreatic neck, duodenum, and right gastroepiploic vessels. The difficulty lies with the standard treatment of the GCT. A medial-to-lateral approach is more in line with the principle of no-touch in tumor surgery and is applied from lower to upper, inside to outside, and left to right, for both the vessels and plane of dissection. Familiarity with vascular variation and the management of vessels in key areas are essential for successful surgery.

Published
2018
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96. Leptin increases chemosensitivity by inhibiting CPT1B in colorectal cancer cells.

Author

Mi X, Yao H, Lu Y, Yang M, Yang Y, Fang D, and He S

Abstract

Background: Chemoresistance is a major cause of treatment failure in advanced colorectal cancer (CRC), severely impacting patient survival and quality of life. While conventional chemotherapy regimens can somewhat control tumor progression, their effectiveness is frequently compromised by the development of drug resistance in cancer cells. The aim of this study is to verify and elucidate the specific mechanisms by which leptin enhances chemosensitivity in CRC, providing valuable insights for the development of new combination chemotherapy options., Methods: We examined the link between CRC chemoresistance and fatty-acid metabolism driven by the high expression of carnitine palmitoyltransferase-1b ( CPT1B ) through an integrated approach combining bioinformatics and clinical sample analysis. In vitro and in vivo experiments were conducted to evaluate the effect of leptin, an adipocyte-derived cytokine, on CRC cells' response to cisplatin., Results: Leptin significantly enhanced CRC cells' chemosensitivity to cisplatin by downregulating CPT1B expression, thereby disrupting the fatty-acid oxidation pathways that support drug resistance. In mouse models, the coadministration of leptin and cisplatin resulted in notable reductions in tumor size and weight compared to cisplatin alone, underscoring leptin's potential to enhance chemotherapy efficacy., Conclusions: These findings indicate that leptin, through modulation of CPT1B , may serve as a promising adjunct to chemotherapy for CRC, addressing the challenge of chemoresistance and improving therapeutic outcomes. The leptin- CPT1B axis may be potential therapeutic target, providing new avenues for CRC treatment strategies aimed at overcoming drug resistance., Competing Interests: Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at https://jgo.amegroups.com/article/view/10.21037/jgo-2024-950/coif). The authors have no conflicts of interest to declare., (2024 AME Publishing Company. All rights reserved.)

Published
2024
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97. Gut microbiota-mediated hsa&#95;circ&#95;0126925 targets BCAA metabolic enzyme BCAT2 to exacerbate colorectal cancer progression.

Author

Yao H, Xu J, Zhou A, Shen D, Dong Q, Yang X, Li M, Mi X, Lu Y, Zhong R, Shi X, Tai Q, Chen G, Shi B, Sun L, Zhou D, Yao Y, and He S

Abstract

Recent evidence indicates that a high-fat diet (HFD) can promote tumor development, especially colorectal cancer (CRC), by influencing the microbiota. Regulatory circular RNAs (circRNAs) play an important role in modulating host-microbe interactions; however, the specific mechanisms by which circRNAs influence cancer progression by regulating these interactions remain unclear. Here, we report that consumption of a HFD modulates the microbiota by specifically upregulating the expression of the noncoding RNA hsa&#95;circ&#95;0126925 (herein referred to as circ&#95;0126925) in CRC. Acting as a scaffold, circ&#95;0126925 hinders the recruitment of the E3 ubiquitin ligase tripartite motif-containing protein 21 (TRIM21) to branched-chain amino acid transaminase 2 (BCAT2), leading to reduced degradation of BCAT2. This reduction in targeted degradation of BCAT2 can protect tumours from limited branched-chain amino acids (BCAAs) interference by improving the metabolism of BCAAs in CRC. Taken together, these data demonstrate that circ&#95;0126925 plays a critical role in promoting the progression of CRC by maintaining BCAA metabolism and provide insight into the functions and crosstalk of circ&#95;0126925 in host-microbe interactions in CRC. Implications: This study preliminarily confirms that circRNAs do indeed respond to microbiota/microbial metabolites, providing further evidence for the potential development of circRNAs as diagnostic tools and/or therapeutic agents to alleviate microbiome related pathology in humans.

Published
2024
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98. Elucidating the Mechanisms of Astragalus Membranaceus in Colorectal Cancer Patients through Bioinformatics Analysis.

Author

Wang S, Tang J, Li G, and He S

Abstract

Background: Astragalus membranaceus has shown positive clinical efficacy in treating colorectal cancer (CRC)., Objective: This study aimed to identify the key active components of Astragalus and determine effective targets of these components in CRC patients., Methods: We identified active components of Astragalus membranaceus and differentially expressed genes in traditional Chinese medicine systems pharmacology database and The Cancer Genome Atlas. Additionally, the enrichment analysis of differential target genes (DTGs) was performed using the R-package clusterProfiler. Immunocyte correlation analysis and non-coding regulatory network construction were performed for biomarkers using Spearman's method and NetworkAnalyst. Finally, molecular docking of biomarkers and their corresponding molecule drugs was done with Autodock Vina software., Results: We identified 20 active components of Astragalus membranaceus and 1 403 target genes through screening. A total of 2 300 differentially expressed genes, and 3 035 hub genes in CRC were screened. The integration of the target genes with the significantly differentially expressed genes and Hub genes identified resulted in a total of 86 DTGs. Subsequently, the results showed 828 enriched GO biological processes, 184 enriched GO molecular functions, 59 enriched GO cellular components, and 46 enriched KEGG pathways. We also obtained a total of 143 PPI pairs involving 67 nodes. Additionally, we constructed 45 mRNA-TF pairs, 101 miRNA-mRNA pairs, and 200 miRNA- mRNA-TF triplets. Finally, molecular docking was performed for the active component quercetin with F2 and UGT1A1 and formic acid with FGA, AHSG, and KNG1., Conclusion: This study identified the active components of Astragalus membranaceus and their corresponding targets in CRC. These findings provide robust evidence for precision drug therapy in patients with CRC., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

Published
2024
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99. Absent in melanoma 2 attenuates proliferation and migration and promotes apoptosis of human colorectal cancer cells by activating P38MAPK signaling pathway.

Author

Zhang Z, Li X, Zhang Y, Zhu H, Qiao Z, Lu Y, Mi X, Cao H, Shen G, and He S

Subjects
Humans, Apoptosis, Cell Proliferation, Interferons, Prospective Studies, Signal Transduction, Colorectal Neoplasms genetics, Melanoma
Abstract

Colorectal cancer (CRC) stands among the top prevalent cancers worldwide and holds a prominent position as a major contributor to cancer-related mortality globally. Absent in melanoma 2 (AIM2), a constituent of the interferon-inducible hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats protein family, contributes to both cancer progression and inflammasome activation. Despite this understanding, the precise biological functions and molecular mechanisms governed by AIM2 in CRC remain elusive. Consequently, this study endeavors to assess AIM2's expression levels, explore its potential antitumor effects, elucidate associated cancer-related processes, and decipher the underlying signaling pathways in CRC. Our findings showed a reduced AIM2 expression in most CRC cell lines. Elevation of AIM2 levels suppressed CRC cell proliferation and migration, altered cell cycle by inhibiting G1/S transition, and induced cell apoptosis. Further research uncovered the participation of P38 mitogen-activated protein kinase (P38MAPK) in AIM2-mediated modulation of CRC cell apoptosis and proliferation. Altogether, our achievements distinctly underscored AIM2's antitumor role in CRC. AIM2 overexpression inhibited proliferation and migration and induced apoptosis of CRC cells via activating P38MAPK signaling pathway, indicating AIM2 as a prospective and novel therapeutic target for CRC., Competing Interests: The authors declare that they have no conflicts of interest to report regarding the present study., (© 2024 Zhang et al.)

Published
2023
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100. [Interferon regulatory factor 5(IRF5) regulates the differentiation of bone marrow-derived macrophages in mice].

Author

Sun K, Qu J, Chen J, Dang S, He S, Zhang J, Xie R, Wang Y, and Zhang J

Subjects
Animals, Arginase metabolism, Cells, Cultured, Interferon Regulatory Factors genetics, Interferon-gamma pharmacology, Lipopolysaccharides, Macrophages drug effects, Macrophages metabolism, Mice, Nitric Oxide Synthase Type II, RNA, Messenger metabolism, Signal Transduction drug effects, Cell Differentiation, Interferon Regulatory Factors metabolism, Macrophages cytology, RNA, Small Interfering metabolism, Tumor Necrosis Factor-alpha metabolism
Abstract

Objective: To investigate the expression differences of interferon regulatory factor 5( IRF5) between M1 and M2 macrophages derived from mouse bone marrow and the effects of small interfering RNA targeting IRF5 gene( IRF5siRNA) on macrophage differentiation., Methods: With the treatment of IFN-γ and lipopolysaccharide( LPS),mouse bone marrow-derived macrophages were differentiated into M1 macrophages; while with the treatment of IL-4,mouse bone marrow-derived macrophages were differentiated into M2 macrophages. Differentiation efficiency was measured by flow cytometry. The expressions of IRF5,IL-12,tumor necrosis factor-α( TNF-α),inducible nitric oxide synthase( i NOS),arginase 1( Arg1),macrophage mannose receptor( MMR) mRNAs in M1 and M2 macrophages were analyzed by quantitative real-time PCR( RT-PCR). Furthermore,macrophages were infected with IRF5 siRNA,and then the mRNA expressions of the above proteins in M1 and M2 were tested by RT-PCR,and the protein expressions were detected by Western blotting.The results were analyzed to evaluate the polarization state of macrophages., Results: The differentiation proportion of macrophages measured by flow cytometry was 81. 7%. RT-PCR showed that the expressions of IRF5,IL-12,i NOS mRNAs were obviously higher in M1 macrophages than in M2,and TNF-α mRNA was also higher in M1 macrophages. The expressions of Arg1,MMR mRNAs were obviously higher in M2 macrophages than in M1 macrophages. After silencing the IRF5 by IRF5 siRNA,the expressions of IRF5, IL-12, TNF-α and i NOS mRNAs decreased remarkably in macrophages, while the expressions of Arg-1,MMR mRNAs increased. Western blotting revealed that the expressions of IRF5,IL-12,TNF-α and i NOS proteins were significantly reduced,while the expressions of Arg1 and MMR protein were raised. The polarization of macrophages shifted to M2 state., Conclusion: IRF5 can be used as an important marker to identify M1 and M2 macrophages,and it has an important role in the regulation of macrophage differentiation.

Published
2017

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