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ObjectivesFor achieving navigational safety and continuous communication link between swarms of unmanned marine vehicle (UMV) during mission execution, the cooperative path planning of unmanned surface vehicle (USV) and unmanned aerial vehicle (UAV) swarms is studied.MethodsKeep-in and keep-out geo-fences are used to carry out scene modelling, and the problems of threat and obstacle avoidance are transformed into geo-fence constraints. Aiming at collision avoidance and continuous communication link between vehicles, a judgment criterion for the constraints of collision avoidance and communication link via time sequence detection is proposed. The average travel time (ATT) of the swarm is taken as the path optimization function, and the multiple constraints are transformed into penalty functions. A self-adaptive differential evolution algorithm is adopted to solve the optimization problem.ResultsThe proposed method can ensure safe navigation and communication link between USV and UAV swarms in hostile and obstacle-filled environment, and achieve the shortest ATT under multiple constraints.ConclusionsThis method has practical value for the off-line path planning of UMV swarms in the hostile and obstacle-filled environment.

The swarm operation of unmanned marine vehicle (UMV) has been developed and evolved from concept to practical application. In view of the mission requirements of UMV swarms, this paper summarizes the development of the UMV swarm concepts of unmanned aerial vehicle (UAV), unmanned surface vehicle (USV) and unmanned underwater vehicle (UUV), and cross-domain swarm operations of UMV in naval warfare. It then analyzes the key technologies of UMV swarm cooperative engagement, including self-organizing communication, collaborative situational awareness, tasking, path planning, formation control and virtual testing. Finally, the main research ideas, representative algorithms and development trends of the related algorithms are summarized systematically. This study can provide valuable references for research on UMV swarm operation technology.

This paper introduces a novel transparent proximity-coupled-fed patch antenna with enhanced impedance bandwidth and good filtering response. The proposed antenna consists of a ground plane, a specific feeding structure and a slotted patch. All the conducting surfaces are realized with metal meshes printed on glass substrates. Optical transparency of the antenna depends on the mesh density. The feed line of a traditional proximity-coupled-fed patch antenna is terminated with a driven stub etched with a half-wavelength U-shaped slot. This modification introduces an additional resonant mode but also a radiating null. By further etching a pair of quarter-wavelength open slots on the radiating patch, another resonant mode accompanied with an extra radiation null is generated. Finally, three resonant modes within the operating band along with two radiation nulls near the two edges of the passband are achieved. The proposed antenna is implemented to demonstrate an impedance bandwidth of 7.6% from 3.41 to 3.68 GHz and a maximum gain of 4.6 dBi.

Abstract Background MNAT1 (menage a trois 1, MAT1), a cyclin-dependent kinase-activating kinase (CAK) complex, highly expressed in diverse cancers and was involved in cancer molecular pathogenesis. However, its deliverance profile and biological function in osteosarcoma (OS) remain unclear. Methods The expression of MNAT1 in OS was detected by western blot (WB) and immunohistochemistry (IHC). The potential relationship between MNAT1 molecular level expression and OS clinical expectations were analyzed according to tissues microarray (TMA). Proliferation potential of OS cells was evaluated in vitro based on CCK8 and OS cells colony formation assays, while OS cells transwell and in situ tissue source wound healing assays were employed to analyze the OS cells invasion and migration ability in vitro. A nude mouse xenograft model was used to detect tumor growth in vivo. In addition, ordinary bioinformatics analysis and experimental correlation verification were performed to investigate the underlying regulation mechanism of OS by MNAT1. Results In this research, we found and confirmed that MNAT1 was markedly over-expressed in OS tissue derived in situ, also, highly MNAT1 expression was closely associated with bad clinical expectations. Functional studies had shown that MNAT1 silencing could weaken the invasion, migration and proliferation of OS cells in vitro, and inhibit OS tumor growth in vivo. Mechanism study indicated that MNAT1 contributed to the progression of OS via the PI3K/Akt/mTOR pathway. We further verified that the MNAT1 was required in the regulation of OS chemo-sensitivity to cisplatin (DDP). Conclusions Taken together, the data of the present study demonstrate a novel molecular mechanism of MNAT1 involved in the formation of DDP resistance of OS cells.

Objective To investigate the safety and effectiveness of endoscopic retrograde cholangiopancreatography (ERCP) in the diagnosis and treatment of pediatric pancreaticobiliary maljunction(PBM). Methods A total of 74 children with PBM diagnosed by ERCP were enrolled. The clinical data with postoperative follow-up were analyzed. Results There were 32 cases with type A, 16 cases with type B, 23 cases with type C and 3 cases with type D based on the classification of PBM proposed by Japanese Study Group on PBM. The positive diagnostic rate of PBM was only 8% using MRCP. All children underwent endoscopic sphincteropapillotomy with endoscopic nasobiliary drain of which 69 cases(93.4%) were preceded with extraction of pancreatic protease emboli or stones. Pancreatic stent was followed in 2 cases. Seven cases (9.5%) had mild pancreatits after ERCP. No bleeding or cardiac laceration and perforations were found. The children had resection of extrahepatic bile duct combined with hepatic duct jejunum Roux-en-Y anastomotisis except for 7(9.5%) cases without biliary dilatation. Conclusions ERCP could be performed safely with effective diagnosis and treatment in pediatric PBM, and is an important procedure for children with PBM during early stage.

The ladybird beetle Serangium japonicum is an important predator of whiteflies. Investigations of the molecular mechanisms of this predatory beetle have been hindered by the scarcity of gene sequence data. To obtain gene sequences for the ladybird beetle and determine differences in gene expression between the summer and winter seasons, paired-end sequencing was performed. Real-time PCR was used to validate differences in Krueppel homolog 1 gene (Kr-h1) mRNA expression in summer vs. winter samples. To determined the diversity of the population, annotated cytochrome c oxidase subunit I gene (COX1) gene fragments were amplified from several ladybird beetle populations. The analysis yielded 191,246 assembled unigenes, 127,016 of which (66.4%) were annotated. These functional annotations of gene sequences are currently available from the National Center for Biotechnology Information (NCBI), and will provide a basis for studying the molecular mechanisms underlying the biological characteristics of S. japonicum. We found a change in expression of ribosome-associated genes across seasons, and postulate that this change is because of seasonal variation in temperature and photoperiod. The differential expression of Kr-h1 suggests that S. japonicum can successfully overwinter because the adults enter diapause. To explain the effects of season on Kr-h1 gene expression, we hypothesize a model in which that a short photoperiod affects the density of Ca2+, the subsequent activity of methyl farnesoate epoxidase and the synthesis of JH, and in turn Kr-h1 gene expression. COX1 annotation was concordant with the morphological ID. The same COX1 sequence was found in the samples from several provinces in China. Therefore, the COX1 sequence is worth further study to distinguish beetle species and populations.

Unmanned surface vessels (USVs) are required to perform motion prediction during a task. This is essential for USVs, especially when conducting motion control, and this work has been proven to be complicated. In this paper, an off-line black box modeling method for USV maneuvering, the Sparrow search algorithm-based weighted-least-squares support vector machine (SSA-WLS-SVM) was proposed to recognize the motion model of a USV. Firstly, the construction of the USV test platform and the processing process of the experimental data were introduced, the correctness of the MMG model was verified using a comparison of the test data and the simulation results, and then the MMG model was used to produce sample data later. To improve the stability and robustness of LS-SVM, weighted least squares and SSA were introduced to perform the optimization of the parameters of the algorithm and its kernel function. Then, the random maneuvering dataset was obtained using simulation on the MMG model, which was then preprocessed and used for training the black-box model. To verify the generalization ability of the identified model, the black-box model was used for comparison analysis between motion prediction with the proposed model and maneuvering test on the USV platform in a scenario different from the training data.

Solute carrier family 7 member 11 (SLC7A11), glutathione peroxidase 4 (GPX4), and apoptosis inducing factor mitochondria associated 2 (AIFM2) are the key regulators in ferroptosis. However, the expression patterns and prognostic roles of these genes in pan-cancer are still largely unclear. The expression patterns and prognostic roles of SLC7A11, GPX4, and AIFM2 and the relationships between the expression levels of these genes and immune infiltration levels in pan-cancer were analyzed by using TIMER, gene expression profiling interactive analysis (GEPIA), Oncomine, and Kaplan–Meier databases. Our results showed that both SLC7A11 and GPX4 were overexpressed in colorectal cancer, and SLC7A11 was overexpressed in lung cancer. High levels of SLC7A11 and AIFM2 were significantly linked with the shortened disease-free survival and overall survival (OS) in adrenocortical carcinoma (ACC), respectively. And high expression of SLC7A11, GPX4, and AIFM2 were significantly correlated with the shortened OS of acute myeloid leukemia patients. In esophageal carcinoma (ESCA), GPX4 expression was significantly associated with the infiltration of macrophage and myeloid dendritic cell, and AIFM2 expression was significantly associated with the infiltration of CD4+ T cell. Importantly, GPX4 expression was positively correlated with the expression levels of monocyte markers (CD14 and CD115) and M2 macrophage markers (VSIG4 and MS4A4A) both in ESCA and in head and neck squamous cell carcinoma (HNSC). In summary, SLC7A11, GPX4, and AIFM2 are dysregulated in many types of cancers, and are candidate prognostic biomarkers for many types of cancers, and can be used to evaluate the infiltration of immune cells in tumor tissues.

Increasing contamination risk from oil/organic liquid leakage creates strong demand for the development of absorbents with excellent hydrophobicity and absorption capacity. Herein, bagasse was carbonized to form porous char with a special structure of array-style and vertically perforated channels, and then the activation process enlarged the pore volume of the char. With the cooperation of low-surface-energy polydimethylsiloxane and diatomaceous earth particles, the modified activated carbon aerogel (MACA) was fabricated by modifying the surface coating and mastoid structure on the bagasse char. Moreover, the MACA demonstrates high porosity oil-water separation, hydrophobicity, and considerable absorption capacity (4.06–12.31 g/g) for gasoline and various organic solvents. This work converts agricultural waste into an efficient porous adsorbent, offering a scalable and commercially feasible solution to solving the leakages of oil/organic solvents.

Abstract Background For coronal shear fractures of humeral capitellum, the lateral approach is the most commonly used surgical approach. However, exposure range of the anterior aspect of the distal humerus is inadequate. The anterolateral approach has also been adopted to overcome this disadvantage. However, this approach seems anatomically complex due to the risk of iatrogenic injury to the radial nerve. So far, the optimal approach for the treatment of capitellar shear fractures remains inconclusive. The purpose of this study is to prospectively review and compare the early clinical and radiographic outcomes of treated with open reduction and Herbert screw internal fixation through the lateral approach or the anterolateral approach. Methods Twenty-six patients with isolated capitellar shear fractures were enrolled from January 2013 to December 2017, and randomly assigned to lateral approach group or anterolateral approach group. All the fractures were treated with open reduction and Herbert screw internal fixation through lateral approach or anterolateral approach. Operation time, wound healing complication, elbow joint function, and radiographic evidence were evaluated and compared between two groups. Results The operation via the anterolateral approach took significantly shorter time than via lateral approach (p 0.05). There is no significant difference with respect to MEPI score of elbow joint between two groups (p > 0.05). Conclusion Based on our findings, both lateral approach and anterolateral approach with Herbert screw internal fixation are suitable for coronal shear fractures of capitellum with satisfactory early outcomes. Compared with the lateral approach, the anterolateral approach made the surgical procedure easier and time saving in current series. When the medial aspect of the trochlea is involved for capitellar coronal fractures, the anterolateral lateral approach should be preferred.

BACKGROUND AND AIMS:Cancer is one of the life-threatening diseases of human beings; the pathogenesis of cancer remains to be further investigated. Toll like receptor (TLR) activities are involved in the apoptosis regulation. This study aims to elucidate the role of Mal (MyD88-adapter-like) molecule in the apoptosis regulation of lung cancer (LC) cells. METHODS:The LC tissues were collected from LC patients. LC cells and normal control (NC) cells were isolated from the tissues and analyzed by pertinent biochemical and immunological approaches. RESULTS:We found that fewer apoptotic LC cells were induced by cisplatin in the culture as compared to NC cells. The expression of Fas ligand (FasL) was lower in LC cells than that in NC cells. FasL mRNA levels declined spontaneously in LC cells. A complex of FasL/TDP-43 was detected in LC cells. LC cells expressed less Mal than NC cells. Activation of Mal by lipopolysaccharide (LPS) increased TDP-43 expression in LC cells. TDP-43 formed a complex with FasL mRNA to prevent FasL mRNA from decay. Reconstitution of Mal or TDP-43 restored the sensitiveness of LC cells to apoptotic inducers. CONCLUSIONS:LC cells express low Mal levels that contributes to FasL mRNA decay through impairing TDP-43 expression. Reconstitution of Mal restores sensitiveness of LC cells to apoptosis inducers that may be a novel therapeutic approach for LC treatment.

Abstract Background Due to the intraarticular and complex nature of the coronal shear fracture of the humeral capitellum and its rarity, it has been difficult to formulate a universally accepted method of surgical management. The purpose of this study is to retrospectively evaluate the clinical outcomes of 15 patients with isolated coronal shear fractures of the capitellum treated by Herbert screw fixation through anterolateral approach, and to address the safety and tips for this surgical procedure. Methods This retrospective study included 15 isolated coronal shear fractures of the capitellum without posterior involvement, which were classified according to the Dubberley classification as 11 type 1A fractures and 4 type 3A fractures. All fractures were treated with Herbert screws fixation via the anterolateral approach. Clinical and radiographic evaluation was performed regularly, with a mean follow-up of 29 months. Results The mean operative time was 81 min. There were no wound healing problems or infection. One incomplete posterior interosseous nerve injury occurred, which recovered soon without residual compromise. All fractures healed well. At the final follow-up, the average range of motion was 134°in flexion-extension and 172°in supination-pronation. There was no significant difference between the affected and the unaffected elbows with regard to motion in flexion-extension or flexion-extension. The average Mayo Elbow Performance Index Score was 93 with 11 excellent and 4 good. No evidence of avascular necrosis, posttraumatic osteoarthritis, or heterotrophic ossification was found. Conclusion Open reduction and internal fixation using Herbert screws through a anterolateral approach is a reliable and effective treatment for coronal shear fractures of capitellum, and able to achieve stable fixation and restoration of a functional range of motion.

Abstract Background The purpose of the study is to investigate whether autologous platelet-rich plasma (PRP) can serve as bone-inducing factors to provide osteoinduction and improve bone regeneration for tissue-engineered bones fabricated with bone marrow mesenchymal stem cells (MSCs) and beta-tricalcium phosphate (β-TCP) ceramics. The current study will give more insight into the contradictory osteogenic capacity of PRP. Methods The concentration of platelets, platelet-derived growth factor-AB (PDGF-AB), and transforming growth factor-β1 (TGF-β1) were measured in PRP and whole blood. Tissue-engineered bones using MSCs on β-TCP scaffolds in combination with autologous PRP were fabricated (PRP group). Controls were established without the use of autologous PRP (non-PRP group). In vitro, the proliferation and osteogenic differentiation of MSCs on fabricated constructs from six rabbits were evaluated with MTT assay, alkaline phosphatase (ALP) activity, and osteocalcin (OC) content measurement after 1, 7, and 14 days of culture. For in vivo study, the segmental defects of radial diaphyses of 12 rabbits from each group were repaired by fabricated constructs. Bone-forming capacity of the implanted constructs was determined by radiographic and histological analysis at 4 and 8 weeks postoperatively. Results PRP produced significantly higher concentration of platelets, PDGF-AB, and TGF-β1 than whole blood. In vitro study, MTT assay demonstrated that the MSCs in the presence of autologous PRP exhibited excellent proliferation at each time point. The results of osteogenic capacity detection showed significantly higher levels of synthesis of ALP and OC by the MSCs in combination with autologous PRP after 7 and 14 days of culture. In vivo study, radiographic observation showed that the PRP group produced significantly higher score than the non-PRP group at each time point. For histological evaluation, significantly higher volume of regenerated bone was found in the PRP group when compared with the non-PRP group at each time point. Conclusions Our study findings support the osteogenic capacity of autologous PRP. The results indicate that the use of autologous PRP is a simple and effective way to provide osteoinduction and improve bone regeneration for tissue-engineered bone reconstruction.

Objective To evaluate the ability of two scoring systems (CHA 2 DS 2 -VASc score and CHA 2 DS 2 -VASc+hyperlipidaemia+smoking [CHA 2 DS 2 -VASc-HS score]) to predict in-stent restenosis (ISR) among patients undergoing drug-eluting stent (DES) implantation. Methods This retrospective study enrolled patients who underwent coronary angiography to assess coronary artery disease severity secondary to a diagnosis of stable angina or acute coronary syndrome that subsequently underwent DES implantations. Demographic, clinical, angiographic and biochemical parameters were compared between those patients that experienced ISR and those that did not during the study follow-up period. Univariate and multivariate logistic regression analyses were used to evaluate associations between the baseline parameters, the two scoring systems and ISR risk. Results A total of 358 patients (non-ISR group n = 316; ISR group n = 42) participated in the study. Compared with the non-ISR group, more patients in the ISR group had diabetes mellitus and received stents with smaller diameters but longer lengths. There were no significant differences with regard the predictive ability for ISR of either the CHA 2 DS 2 -Vasc or the CHA 2 DS 2 -Vasc-HS scores. Multivariate logistic regression analyses demonstrated that stent diameter, follow-up duration and glycosylated haemoglobin were independent risk factors for ISR. Conclusions The CHA 2 DS 2 -Vasc and CHA 2 DS 2 -Vasc-HS scores did not predict ISR in patients after coronary DES placement.

We propose and numerically analyze a fiber-optic sensor based on a silica taper-assisted multiple polymer microspheres to realize high-sensitivity refractometric sensing due to the excitation of multi-beam interference and whispering-gallery modes (WGMs) in each microsphere. Up to 5 UV-cured adhesive microspheres are dripped in sequence on the 2 µm-diameter silica taper with the certain distances in between. Scattering and reflection of light in each microsphere enhance the multi-beam interference because of the emergence of high-order modes. Moreover, WGMs with certain resonant wavelengths are excited in the microspheres, further enhancing the transmission spectrum with high contrast and quality factor. Furthermore, irregular transmission spectrum corresponds to the large wavelength tuning range. Propagating light between the microspheres could be strongly affected by the environmental factor with the large transmission spectrum shift, therefore realizing the refractometric sensing with high sensitivity of 846 nm/RIU based on the taper-assisted 2-spheres sensor. Furthermore, another two microspheres are placed on both sides of 2-spheres sensor to form 4-spheres-loop sensor, re-collecting those large-angle scattering light with the enhancement of multi-beam interference and WGMs. Therefore, the sensitivity of 4-shperes-loop sensor is further improved to the maximum of 1296.3 nm/RIU.