51. Characterization and comparison of the bacterial community between complete intensive and extensive feeding patterns in pigs
- Author
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Yahui Xue, Ye Jianwei, Li Xinjian, Wang Kejun, Han Xuelei, Qiao Ruimin, Xiuling Li, Dongdong Duan, Cong Li, and Mingyu Wang
- Subjects
Microbial diversity ,food.ingredient ,lcsh:Biotechnology ,lcsh:QR1-502 ,Biophysics ,Gut flora ,Applied Microbiology and Biotechnology ,lcsh:Microbiology ,Coprococcus ,Microbiology ,03 medical and health sciences ,Feeding patterns ,food ,lcsh:TP248.13-248.65 ,Gammaproteobacteria ,Gene expression ,16S rRNA ,Feces ,030304 developmental biology ,0303 health sciences ,Gut microbiome ,Pig ,biology ,030306 microbiology ,16S ribosomal RNA ,biology.organism_classification ,Isolation (microbiology) ,Original Article ,Bacteria - Abstract
To investigate and compare the gut microbiota structures in complete intensive feeding pattern (CP) and extensive feeding pattern (EP) groups, a total of 20 pigs were divided into two groups and fed the same diet. The fecal microbial composition was profiled using 16S rRNA gene sequencing. Our results showed that seventeen predominant genera were present in each pig sample and constituted the phylogenetic core of the microbiota at the class level. The abundance of most of the core microbial flora were significantly higher in the CP group than in the EP group (P Gammaproteobacteria was significantly lower in the CP group than in the EP group (P P Coprococcus, which is a key gut bacterium in the intestine, was selected for isolation and purification and cocultured with intestinal epithelial cells. qPCR was performed to determine the effect of Coprococcus on SLA-DRB gene expression in intestinal epithelial cells. The results showed that Coprococcus enhanced SLA-DRB gene expression in intestinal epithelial cells. The results provide useful reference data for further study on the relationship between intestinal flora and pig disease resistance.
- Published
- 2021