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Plant salt tolerance is controlled by various physiological processes such as water and ion homeostasis, photosynthesis, and cellular redox balance, which are in turn controlled by gene expression. In the present study, plants of six canola cultivars (DGL, Dunkled, Faisal Canola, Cyclone, Legend, and Oscar) were evaluated for salt tolerance by subjecting them to 0 or 200 mM NaCl stress. Based on growth, cultivars DGL, Dunkled, and Faisal Canola were ranked as salt tolerant, while cultivars Cyclone, Legend, and Oscar were ranked as salt-sensitive ones. Differential salt tolerance in these canola cultivars was found to be associated with a relatively lower accumulation of Na+ and greater accumulation of K+ in the leaves, lower oxidative damage (MDA), and better antioxidative defense system (Superoxide dismutase, SOD; peroxidase, POD, and catalase, CAT). Cultivar Oscar was the poorest to discriminate Na+ and K+ uptake and accumulation in leaves and had poor antioxidant potential to scavenge ROS. Salt stress did not affect the structural stability of photosystem-II (PSII) till three weeks, thereafter it caused a significant decrease. Salt stress increased the performance index (PIABS) by increasing the density of active reaction centers in Oscar. Salt stress decreased the antenna size thereby lowering the absorption and trapping energy flux, and maintaining the electron transport with an increase in heat dissipation. This may represent a potential mechanism to cope with salt stress. Transcriptome analysis of salt-sensitive cultivar Oscar further revealed that salt stress down-regulated DEGs related to hormonal signal transduction pathways, photosynthesis, and transcription factors, while DEGs related to the biosynthesis of amino acid and ion transport were up-regulated. In conclusion, salt tolerance in canola cultivars was associated with ion exclusion and maintenance of photosynthesis. Salt stress sensitivity in cultivar Oscar was mainly associated with poor control of ion homeostasis which caused oxidative stress and reduced photosynthetic efficiency. [ABSTRACT FROM AUTHOR]

Vitamin D is an important nutrient for bone health, and vitamin D deficiency increases the risk of various diseases. Gilgit Baltistan, the northern-most area of Pakistan, has a high prevalence of vitamin D deficiency, despite many nutritional and food safety programmes. The present study aimed to find how knowledge, attitudes and practices associated with vitamin D related to the prevalence of vitamin D deficiency among people residing in different areas of Gilgit Baltistan. The cross-sectional study was descriptive and used data from a survey carried out between February 2019 and December 2020 on individuals of both sexes aged 10 years or over in Gilgit Baltistan. Of the 575 survey participants, 306 (53.2%) had experienced signs and symptoms of vitamin D deficiency, i.e. tiredness, fatigue and bone weakness. Approximately 64.8% had some general knowledge of vitamin D and its relation to health. Participants aged 19-25 years had the highest scores on knowledge of vitamin D. Only 22.7% of interviewees had ever taken any supplements and only 25.6% often exposed themselves to sunlight. Females' mean knowledge score (28.7; SD 7.02) was higher than that of males (24; SD 9.01). A lack of consistency was observed between attitude towards daylight exposure and knowledge of vitamin D. There was a large correlation between knowledge and attitude (p = 0.001), while a non-significant association was demonstrated between knowledge and practices (p = 0.1). Better knowledge, attitude and practices by people living in cities or more-developed regions indicates that education can be an effective way to provide awareness regarding micronutrient deficiencies. More emphasis is needed on enhancing knowledge, awareness and practices associated with vitamin D deficiency in rural areas of Pakistan. It is strongly recommended that an awareness campaign on micronutrients is launched in both rural and urban areas of Pakistan, concentrating on poor socioeconomic settings. [ABSTRACT FROM AUTHOR]

Additive manufacturing (AM) of metallic alloys offers a new avenue to print objects having complex geometries. This exclusive benefit of AM has made it an alternative route to conventional manufacturing. Importantly, additively manufactured (AMed) alloys often exhibit improved microstructures, which may provide better properties. The microstructure of an alloy can be tuned by controlling the processing parameters. This study includes an overview of the processing parameters that can influence the microstructural, mechanical, and corrosion properties of AMed alloys. Moreover, the effects of heat treatment on AMed alloys are also discussed. Among various processing parameters, it is observed that the laser power significantly influences the microstructure. The microstructures produced with high laser power are similar to heat-treated samples for 316L stainless steel (SS) and Ti6Al4V. Similarly, variation in scanning speed results in distinct morphology of grains in Ti6Al4V. Moreover, different AM processes, such as SLM and EBM, produce coarse and fine β grains, respectively, in Ti6Al4V. The fabrication of AlSi10Mg yields various sizes of melt pool due to different scanning strategies. Furthermore, mechanical properties such as microhardness is higher and the yield strength is lower for Ti6Al4V fabricated at lower laser power. The corrosion behavior of SLMed Ti6Al4V is different on the perpendicular and parallel planes to the build direction. Due to the increase in grain size after heat treatment, the corrosion resistance of AMed Ti6Al4V and AlSi10Mg is reduced. In contrast, heat treatment applied on 316L, Ti6Al4V, AlSi10Mg, and Inconel 718 is beneficial for mechanical properties. After the development of materials with optimized processing parameters, the research should be conducted on replacement of the wrought alloys with the AMed alloys. It is expected that new applications such as fuel cells and biomedical devices will utilize the AM technology to build parts in the recent future. [ABSTRACT FROM AUTHOR]