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Born-again planetary nebulae (PNe) are extremely rare cases in the evolution of solar-like stars. It is commonly accepted that their central stars (CSPN) experienced a very late thermal pulse (VLTP), ejecting H-deficient material inside the evolved H-rich PN. Given the short duration of this event and the fast subsequent evolution of the CSPN, details of the mass ejection are unknown. We present the first morphokinematic model of the H-deficient material surrounding a born-again PN, namely A 30. New San Pedro Mártir observations with the Manchester Echelle Spectrograph were recently obtained to map the inner region of A 30 which are interpreted by means of the software SHAPE in conjunction with HST WFC3 images. The SHAPE morphokinematic model that best reproduces the observations is composed by a disrupted disc tilted 37° with respect to the line of sight and a pair of orthogonal opposite bipolar ejections. We confirm previous suggestions that the structures closer to the CSPN present the highest expansion velocities, that is, the disrupted disc expands faster than the farther bipolar features. We propose that the current physical structure and abundance discrepancy of the H-deficient clumps around the CSPN of A 30 can be explained by a common envelope phase following the VLTP event. Our proposed scenario is also compared with other known born-again PNe (A 58, A 78, HuBi 1, and the Sakurai’s Object). © 2022 The Author(s). Published by Oxford University Press on behalf of Royal Astronomical Society., JBR-G, ES, and GR acknowledge support Consejo Nacional de Ciencia y Tecnología (CONACyT, Mexico) for a student scholarship. JAT acknowledges funding from the Marcos Moshinsky Foundation (Mexico) and Dirección General de Asuntos del Personal Académico (DGAPA), Universidad Nacional Autónoma de México, through grants Programa de Apoyo a Proyectos de Investigación e Inovación Tecnológica (PAPIIT) IA101622. JAT also acknowledges support from the ‘Center of Excellence Severo Ochoa’ Visiting-Incoming program. JAT and GR-L acknowledge support from CONACyT (grant 263373). MAGR and BMM acknowledge support from the State Agency for Research of the Spanish MCIU through the ‘Center of Excellence Severo Ochoa’ award to the Instituto de Astrofísica de Andalucía (SEV-2017-0709). Y-HC acknowledges the support of MOST grant 110-2112-M-001-020 from the Ministry of Science and Technology of Taiwan. LS acknowledges support from UNAM DGAPA PAPIIT Grant IN110122 (Mexico). This paper is based in part on ground-based observations from the Observatorio Astronómico Nacional at the Sierra de San Pedro Mártir (OAN-SPM), which is a national facility operated by the Instituto de Astronomía of the Universidad Nacional Autónoma de México. The authors thank the telescope operator P. F. Guillén for valuable guidance during several observing runs, and to the OAN-SPM staff for their valuable support. This work has made extensive use of the NASA’s Astrophysics Data System.

Delivery of functional proteins into the intracellular space has been a challenging task that could lead to a myriad of therapeutic applications. We report herein a novel bioconjugation strategy for enzyme modification and selective delivery into cancer cells for lock-and-key-type activation of photosensitizers. Using a bifunctional linker containing a bis(bromomethyl)phenyl group and an

Background Understanding nursing students’ knowledge about and attitudes toward older adults’ using context-specific survey instruments can help to identify and design effective learning and teaching materials to improve the care for persons 60 years and above. However, there are no validated instruments to examine nursing students’ knowledge and attitudes toward the care for older adults in the African context. The study aimed to evaluate the items on the Knowledge about Older Patients Quiz and Kogan's Attitudes towards Old People Scale suitable for the African context. Methods A cross-sectional study was conducted using second-and third-year nursing students from two public Nursing Training Institutions in Ghana. Using Sahin’s rule of sample size estimate of at least 150 participants for unidimensional dichotomous scales, 170 nursing students were recruited to participate after an information session in their classrooms. Data were collected from December 2019—March 2020 using the Knowledge about Older Patients Quiz and Kogan’s Attitudes Towards Old People Scale. Item response theory was employed to evaluate the Knowledge about Older Patients Quiz difficulty level and discrimination indices. Corrected item-to-total correlation analysis was conducted for Kogan's Attitudes towards Old People Scale. The internal consistency for both scales was examined. Results Of the 170 participants, 169 returned completed surveys. The mean age of participants was 21 years (SD = 3.7), and (54%) were female. Of the 30-items of the Knowledge about Older Patients Quiz, seven items were very difficult for most students to choose the correct response, and one was easy, as most of the students chose the correct response. Although 22 items demonstrated appropriate difficulty level, discrimination indices were used to select the final 15- items that discriminated moderately between upper and lower 25% performing students. The Kuder-Richardson-20 reliability was. 0.30, which was low. Considering Kogan's Attitudes towards Old People scale, 10-items were removed following negative and low corrected item-to-total correlation and a high Alpha coefficient if items were deleted. The final 22-items had a Cronbach alpha coefficient of 0.65, which was moderately satisfactory. Conclusion Evaluation of the scales demonstrated essential content validity and moderate internal consistency for the context of our study. Further research should focus on ongoing context-specific refinement of the survey instruments to measure nursing students’ knowledge about and attitudes toward caring for older adults in the African context.

CD8 tissue-resident memory T (TRM) cells provide frontline protection at barrier tissues; however, mechanisms regulating TRM cell development are not completely understood. Priming dictates the migration of effector T cells to the tissue, while factors in the tissue induce in situ TRM cell differentiation. Whether priming also regulates in situ TRM cell differentiation uncoupled from migration is unclear. Here, we demonstrate that T cell priming in the mesenteric lymph nodes (MLN) regulates CD103+ TRM cell differentiation in the intestine. In contrast, T cells primed in the spleen were impaired in the ability to differentiate into CD103+ TRM cells after entry into the intestine. MLN priming initiated a CD103+ TRM cell gene signature and licensed rapid CD103+ TRM cell differentiation in response to factors in the intestine. Licensing was regulated by retinoic acid signaling and primarily driven by factors other than CCR9 expression and CCR9-mediated gut homing. Thus, the MLN is specialized to promote intestinal CD103+ CD8 TRM cell development by licensing in situ differentiation.

RATIONALE: Current guidelines do not sufficiently capture the heterogeneous nature of asthma; a more detailed molecular classification is needed. Metabolomics represents a novel and compelling approach to derive asthma endotypes (i.e., subtypes defined by functional and/or pathobiological mechanisms). OBJECTIVES: To validate metabolomic-driven endotypes of asthma and explore their underlying biology. METHODS: In the Genetics of Asthma in Costa Rica Study (GACRS), untargeted metabolomic profiling, similarity network fusion, and spectral clustering was used to identify metabo-endotypes of asthma, and differences in asthma-relevant phenotypes across these metabo-endotypes were explored. The metabo-endotypes were recapitulated in the Childhood Asthma Management Program (CAMP), and clinical differences were determined. Metabolomic drivers of metabo-endotype membership were investigated by meta-analyzing findings from GACRS and CAMP. MEASUREMENTS AND MAIN RESULTS: Five metabo-endotypes were identified in GACRS with significant differences in asthma-relevant phenotypes, including prebronchodilator (p-ANOVA = 8.3 × 10(−5)) and postbronchodilator (p-ANOVA = 1.8 × 10(−5)) FEV(1)/FVC. These differences were validated in the recapitulated metabo-endotypes in CAMP. Cholesterol esters, trigylcerides, and fatty acids were among the most important drivers of metabo-endotype membership. The findings suggest dysregulation of pulmonary surfactant homeostasis may play a role in asthma severity. CONCLUSIONS: Clinically meaningful endotypes may be derived and validated using metabolomic data. Interrogating the drivers of these metabo-endotypes has the potential to help understand their pathophysiology.

BACKGROUND Studies have shown that mobile applications (apps) have the potential to serve as a non-pharmacological intervention for dementia care and improve the quality of life of people living with dementia (PLwD) and their informal caregivers. However, little is known about the needs and privacy aspects of these mobile apps in dementia care. OBJECTIVE This review sought to understand the landscape of existing mobile apps in dementia care for PLwD and their caregivers with respect to app features, usability testing, privacy and security. METHODS An extensive database search was conducted. Records were independently screened, and two reviewers abstracted data. Findings were summarized and tabulated based on each research aim. RESULTS Forty-four studies were included in this review, with 39 published after 2015. Fifty apps were included in the study, with more apps developed for PLwD as end-users compared to caregivers. Eight themes that contributed to app usability were found. Five methods (questionnaires, interviews, observations, logging, and focus groups) were used to evaluate usability. The reliability of usability testing may be improved by using objective measures. There was little focus on the privacy and security aspects, including the data transfer and protection, of mobile applications for PLwD. CONCLUSIONS This review highlighted key issues related to usability, privacy and security of existing mobile apps for PLwD and their caregivers. To advance future work, our findings provide a guiding framework to app developers/researchers for their privacy policy generation, app development strategy, and usability testing of their app. CLINICALTRIAL CRD42020216141 INTERNATIONAL REGISTERED REPORT RR2-10.1159/000514838

Porous MnO2 microflowers with a hexagonal crystalline structure were facilely prepared at a low hydrothermal temperature of 90°C, without using any template or capping agent. The as-prepared MnO2 only presented an excellent detection ability for copper (II) by a square wave anodic stripping voltammetry in the presence of super P carbon black as conducting agent, and Nafion as binder. In the present work, to evaluate the detection ability of copper (II) in the MnO2 microflowers, chips of screen-printed electrodes (SPEs) having a polyurethane substrate, a silver working electrode, a carbon counter electrode, and a silver pseudoelectrode, were designed. Then, the SPEs chips were modified with MnO2 microflowers and/or super P carbon and used as electrochemical sensors for the detection of copper (II) present in water sources. From the measured results, the fabricated sensors with excellent copper detection in a linear range from 0.625 nM to 15 nM (R2 = 0.9737), and a low detection limit (0.5 nM), high sensitivity (214.05 μA/cm2 nM), and rapid response (180 s) demonstrated high application potential for electrochemical sensors in the detection of copper in water resources.

A recent analysis of Chandra X-ray data of the metal-polluted white dwarf (WD) G29-38 has revealed X-ray emission that can be attributed to the accretion of debris from a planetary body. In the light of this detection we revisit here archival XMM-Newton observations of G29-38 from which only an upper limit was derived in the past due to the presence of a relatively bright nearby X-ray source. An analysis of these data in multiple energy bands allows disentangling the X-ray emission at the location of G29-38 from that of the nearby source. The similar spectral properties of the source in the XMM-Newton and Chandra observations and their spatial shift, consistent with the proper motion of G29-38 between these observations, strengthen the origin of that X-ray emission from G29-38. The X-ray luminosities from both observations are consistent within 1-$\sigma$ uncertainties, so too are the best-fit plasma temperatures. Although the count number is small, there is tantalizing evidence for line emission in the 0.7-0.8 keV energy band from an optically-thin hot plasma. The most likely candidate for this line emission would be the Fe complex at 16 \r{A}., Comment: 6 pages, 4 figures, 1 table; accepted to ApJL

Introduction Hemolysis during pediatric extracorporeal membrane oxygenation (ECMO) is associated with increased risk for renal failure and mortality. Objectives We aim to describe risk factors for hemolysis in pediatric ECMO supported by centrifugal pumps. Methods We conducted an analysis of retrospective data collected at an academic children’s hospital from January 2017 to December 2019. Measurements and results Plasma-free hemoglobin (PFH) levels were measured daily, and hemolysis was defined as PFH>50 mg/dL. Of 46 ECMO runs over 528 ECMO days, hemolysis occurred in 23 (58%) patients over a total of 40 (8%) ECMO days. In multivariable logistic regression models, VA-ECMO (aOR=4.69, 95% CI: 1.01–21.83) and higher hemoglobin (aOR = 1.38, 95% CI: 1.06–1.81) were independently associated with hemolysis. There were also non-significant trends toward increased risk for hemolysis with higher rotational pump speed (aOR=2.39, 95% CI: 0.75–7.65), higher packed red blood cell transfusions (aOR=1.15, 95% CI: 0.99–1.34), and higher cryoprecipitate transfusions (aOR=2.01, 95% CI: 0.83–4.86). Isolated pump exchanges that were performed in 12 patients with hemolysis led to significant decreases in PFH levels within 24 h (89 vs 11 mg/dL, pConclusions Hemolysis is common in pediatric ECMO using centrifugal pumps. Avoidance of high pump speeds and conservative administration of blood products may help to mitigate the risk for hemolysis. Furthermore, pump exchange may be an effective first-line treatment for hemolysis.

Background: Aging is often associated with increasing functional decline as measured by deterioration in mobility and activities of daily living. Older adults (OAs) living in residential long-term care (LTC) homes in particular may not engage in regular physical exercise, significantly increasing their risk of further cognitive and functional decline. Exergaming may hold promise for OAs by combining exercise and technology-based gaming systems, but evidence for its use in LTC is unknown. Methods: A systematic review was conducted to summarize the effects of exergaming interventions on physical, cognitive, and quality of life (QoL) outcomes for OAs (>65 years of age) living in LTC. Results: Twenty-one studies involving 657 OAs living in LTC met the inclusion criteria. Most studies were associated with a high risk of bias and many used uncontrolled designs and small samples. Across studies, exergame interventions were associated with preliminary benefits relative to control conditions on standardized measures of physical outcomes (e.g., Timed Up & Go, 5-meter gait speed). No consistent effects were found for cognitive and QoL outcomes. Conclusions: Exergames might be a promising intervention to benefit the physical health of OAs (>65 years) living in LTC, but more research is required to determine the effects of exergaming on physical health, as well as cognitive and QoL outcomes. More specifically, larger and more methodologically robust evaluations are needed.

This review article focuses on the dermatologic manifestations of selected nutrient deficiencies, including protein-energy and micronutrient-related malnutrition. The various nutrient deficiencies presented may share common features. However, distinctive cutaneous signs may prompt clinicians to consider a nutritional cause and help distinguish a nutrient deficiency from other common dermatologic conditions. The recent reemergence of forgotten nutritional deficiencies, such as scurvy and pellagra, in the context of predisposing risk factors that may uniquely affect women more than men makes this topic timely. Recognition of nutritional disorders is important because appropriate treatment may reverse cutaneous signs and prevent irreversible sequelae.

Objective Assess the association between depression among new long-term care residents (

The majority of patients who sustained small (low % total body surface area [TBSA]) burns are assessed in an outpatient setting. This can be a traumatic experience, particularly, for paediatric patients. During the initial assessment pharmacological and non-pharmacological adjuncts, such as distraction therapy, can be employed to provide an environment that may reduce and minimise distress. In this study, we investigated whether distraction therapy reduces objective pain scores during the outpatient assessment of small acute burns in children.Two cohorts of patients were formed. In the first group (group A), the children received analgesia and routine nursing care. In the second group (group B), the children received specialist distraction therapy in addition to the same analgesia and nursing care. We recorded patient demographics, TBSA, anatomical sites and mechanism of burn. The Wong Baker™ visual analogue scale (VAS) was used to convert perceived pain, as reported by the patient or parent, into a numerical value at three set intervals during the consultation.50 patients were recruited with 32 patients in group A and 18 in group B. A larger TBSA positively correlated with higher pain scores (p0.05). At the start of the consultation the mean pain score without distraction therapy was 1.55 and with specialist distraction therapy was 0.33 (p0.05). The mean peak pain score during dressing change without distraction therapy was 6.80 and with specialist distraction was 4.47 (p0.05). At the end of the procedure, the mean pain score was 2.12 without distraction therapy and with specialist distraction 1.75.This study of pain scores in small acute paediatric burns has shown that distraction therapy provided by a qualified play specialist can reduce maximal pain by over 2 points on the Wong-Baker™ VAS. Consideration should be given to ensure that distraction therapy is available at all times during initial consultations for children who have sustained small burns.

It is generally believed that epitaxial thin films are subject to compressive strain by the nearest layer with a smaller lattice constant or to tensile strain by the nearest layer with a larger lat...

BACKGROUND AND AIM Protein O-GlcNAcylation is a critical post-translational modification regulating gene expression and fundamental cell functions. O-GlcNAc transferase (OGT) emerged as a key regulator of liver pathophysiology and disease. In this study, we aimed to evaluate the role of OGT in hepatic stellate cells (HSCs) and its consequent role in liver fibrosis. METHODS Primary HSCs were isolated from C57/B6 mice. Cell morphology and α-SMA immunofluorescence staining were observed under scanning confocal microscope. Transcriptomic profile was evaluated by RNAseq (Illumina). Promoter activity was examined by luciferase and β--Galactosidase reporter assays. Liver fibrosis mouse models were induced either by intraperitoneal injection of CCl4 at 3 times/week for 4 weeks or by feeding with methionine and choline deficient (MCD) diet for 4 weeks. RESULTS OGT protein expression and protein O-GlcNAcylation were significantly decreased in CCl4 - or MCD diet-induced liver fibrosis as compared with normal liver in mice. OGT expression and protein O-GlcNAcylation were also decreased in primary HSCs isolated from liver with CCl4 -induced fibrosis compared with those from normal liver. RNA-seq showed that OGT knockdown in HSCs modulated key signaling pathways involved in HSC activation. Promoter sequence analysis of the differentially expressed genes predicted serum response factor (SRF) as a key transcription factor regulated by OGT. Luciferase reporter assay confirmed that OGT repressed activity of SRF to induce α-SMA transcription. Mutations of specific O-GlcNAcylation sites on SRF increased its transcriptional activity, validating negative regulation of SRF by OGT-mediated O-GlcNAcylation. CONCLUSIONS Our results suggest that OGT functions as a negative regulator of HSC activation by promoting SRF O-GlcNAcylation to protect against liver fibrosis.

Background The COVID-19 pandemic and subsequent lockdown measures have led to increasing mental health concerns in the general population. We aimed to assess the short-term impact of the pandemic lockdown on mental health emergency services use in the Kitchener-Waterloo region of Ontario, Canada. Methods We conducted an observational study during the 6-month period between March 5 and September 5, 2020 using National Ambulatory Care Reporting System metadata from mental health visits to three regional Emergency Departments (ED); mental health and substance related police calls; and calls to a regional mental health crisis telephone line, comparing volumes during the pandemic lockdown with the same period in 2019. Quasi-Poisson regressions were used to determine significant differences between numbers of each visit or call type during the lockdown period versus the previous year. Significant changes in ED visits, mental health diagnoses, police responses, and calls to the crisis line from March 5 to September 5, 2020 were examined using changepoint analyses. Results Involuntary admissions, substance related visits, mood related visits, situational crisis visits, and self-harm related mental health visits to the EDs were significantly reduced during the lockdown period compared to the year before. Psychosis-related and alcohol-related visits were not significantly reduced. Among police calls, suicide attempts were significantly decreased during the period of lockdown, but intoxication, assault, and domestic disputes were not significantly different. Mental health crisis telephone calls were significantly decreased during the lockdown period. There was a significant increase in weekly mental health diagnoses starting in the week of July 12 – July 18. There was a significant increase in crisis calls starting in the week of May 31 – June 6, the same week that many guidelines, such as gathering restrictions, were eased. There was a significant increase in weekly police responses starting in the week of June 14 – June 20. Conclusions Contrary to our hypothesis, the decrease in most types of mental health ED visits, mental health and substance-related police calls, and mental health crisis calls largely mirrored the overall decline in emergency services usage during the lockdown period. This finding is unexpected in the context of increased attention to acutely deteriorating mental health during the COVID-19 pandemic.

Targeted delivery and specific activation of photosensitizers can greatly improve the treatment outcome of photodynamic therapy. To this end, we report herein a novel dual receptor-mediated bioorthogonal activation approach to enhance the tumor specificity of the photodynamic action. It involves the targeted delivery of a biotinylated boron dipyrromethene (BODIPY)-based photosensitizer, which is quenched in the native form by the attached 1,2,4,5-tetrazine unit, and an epidermal growth factor receptor (EGFR)-targeting cyclic peptide conjugated with a bicycle[6.1.0]non-4-yne moiety. Only for cancer cells that overexpress both the biotin receptor and EGFR, the two components can be internalized preferentially where they undergo an inverse electron-demand Diels-Alder reaction, leading to restoration of the photodynamic activity of the BODIPY core. By using a range of cell lines with different expression levels of these two receptors, we have demonstrated that this stepwise "deliver-and-click" approach can confine the photodynamic action on a specific type of cancer cells.

Maternal infection and stress during the prenatal period have been associated with adverse neurodevelopmental outcomes in offspring, suggesting that biomarkers of increased inflammation in the mothers may associate with poorer developmental outcomes. In 491 mother–child pairs from the Vitamin D Antenatal Asthma Reduction Trial (VDAART), we investigated the association between maternal levels of two inflammatory biomarkers; interleukin-8 (IL-8) and C-Reactive Protein (CRP) during early (10–18 wks) and late (32–38 wks) pregnancy with offspring scores in the five domains of the Ages and Stages Questionnaire, a validated screening tool for assessing early life development. We identified a robust association between early pregnancy IL-8 levels and decreased fine-motor (β: −0.919, 95%CI: −1.425, −0.414, p = 3.9 × 10−4) and problem-solving skills at age two (β: −1.221, 95%CI: −1.904, −0.414, p = 4.9 × 10−4). Associations between IL-8 with other domains of development and those for CRP did not survive correction for multiple testing. Similarly, while there was some evidence that the detrimental effects of early pregnancy IL-8 were strongest in boys and in those who were not breastfed, these interactions were not robust to correction for multiple testing. However, further research is required to determine if other maternal inflammatory biomarkers associate with offspring neurodevelopment and work should continue to focus on the management of factors leading to increases in IL-8 levels in pregnant women.

BackgroundDuring the coronavirus (COVID-19) pandemic, long-term care homes (LTCHs) imposed visitor restrictions that prevented essential family caregivers (EFCs) from entering the homes. Under these policies, EFCs had to engage in virtual, window, and outdoor visits, prior to the re-initiation of indoor visits.ObjectiveTo understand EFCs’ visitation experiences with LTCH residents during COVID-19.MethodsSeven virtual focus groups with EFCs were conducted and analysed using a thematic approach.FindingsSix themes were identified: (a) inconsistent and poor communication; (b) lack of staffing and resources; (c) increasing discord between EFCs and staff during COVID-19; (d) shock related to reunification; (e) lack of a person-centred or family-centred approach; and, (f) EFC and resident relationships as collateral damage.DiscussionOur findings reflect how EFCs’ visitation experiences were affected by factors at the individual, LTCH, and health-system levels. Future sectoral responses and visitation guidelines should recognize EFCs as an integral part of the care team.

Massive stars are among the main cosmic engines driving the evolution of star-forming galaxies. Their powerful ionising radiation and stellar winds inject a large amount of energy in the interstellar medium. Furthermore, mass-loss ($\dot{M}$) through radiatively driven winds plays a key role in the evolution of massive stars. Even so, the wind mass-loss prescriptions used in stellar evolution models, population synthesis, and stellar feedback models often disagree with mass-loss rates empirically measured from the UV spectra of low metallicity massive stars. The most massive young star cluster in the low metallicity Small Magellanic Cloud galaxy is NGC346. This cluster contains more than half of all O stars discovered in this galaxy so far. A similar age, metallicity ($Z$), and extinction, the O stars in the NGC346 cluster are uniquely suited for a comparative study of stellar winds in O stars of different subtypes. We aim to use a sample of O stars within NGC346 to study stellar winds at low metallicity. We mapped the central 1" of NGC346 with the long-slit UV observations performed by the Space Telescope Imaging Spectrograph (STIS) on board of the {\em Hubble Space Telescope} and complemented these new datasets with archival observations. Multi-epoch observations allowed for the detection of wind variability. The UV dataset was supplemented by optical spectroscopy and photometry. The resulting spectra were analysed using a non-local thermal equilibrium model atmosphere code (PoWR) to determine wind parameters and ionising fluxes. The effective mapping technique allowed us to obtain a mosaic of almost the full extent of the cluster and resolve stars in its core. Among hundreds of extracted stellar spectra, 21 belong to O stars. Nine of them are classified as O stars for the first time. We analyse, in detail, the UV spectra of 19 O stars... (continued), Comment: 33 pages, 40 figures

The larvae of Echinococcus (hydatidcyst) can parasitize humans and animals, causing a serious zoonotic disease-echinococcosis. The life history of Echinococcus is complicated, and as the disease progresses slowly after infection, early diagnosis is difficult to establish. Due to the limitations of imaging and immunological diagnosis in this respect, domestic and foreign scholars have established a variety of molecular detection techniques for the pathogen Echinococcus over recent years, mainly including nested polymerase chain reaction (PCR), multiplex PCR, real-time quantitative PCR, and nucleic acid isothermal amplification technology. In this article, the research progress of molecular detection technology for Echinococcus infection currently was reviewed and the significance of these methods in the detection and diagnosis of hydatid and hydatid diseases was also discussed.

Pregnancy is defined as a 'red flag' in headache assessment. We aimed to describe the prevalence and causes of serious secondary headache in pregnant ED patients.Unplanned secondary analysis of HEAD Study/HEAD Colombia data.3.2% (117/3643) of ED headache patients aged 18-50 years were pregnant, of whom six (5.1%) had a serious secondary cause identified. The proportion of patients with serious headache causes was not significantly different between pregnant female, non-pregnant female and male patient subgroups (P = 0.89).Inclusion of pregnancy as a 'red flag' in ED headache assessment is not supported by these data.

IntroductionThere has been growing interest in using real-time location systems (RTLS) in residential care settings. This technology has clinical applications for locating residents within a care unit and as a nurse call system, and can also be used to gather information about movement, location, and activity over time. RTLS thus provides health data to track markers of health and wellbeing and augment healthcare decisions. To date, no reviews have examined the potential use of RTLS data in caring for older adults with cognitive impairment living in a residential care setting.ObjectiveThis scoping review aims to explore the use of data from real-time locating systems (RTLS) technology to inform clinical measures and augment healthcare decision-making in the care of older adults with cognitive impairment who live in residential care settings.MethodsEmbase (Ovid), CINAHL (EBSCO), APA PsycINFO (Ovid) and IEEE Xplore databases were searched for published English-language articles that reported the results of studies that investigated RTLS technologies in persons aged 50 years or older with cognitive impairment who were living in a residential care setting. Included studies were summarized, compared and synthesized according to the study outcomes.ResultsA total of 27 studies were included. RTLS data were used to assess activity levels, characterization of wandering, cognition, social interaction, and to monitor a resident’s health and wellbeing. These RTLS-based measures were not consistently validated against clinical measurements or clinically important outcomes, and no studies have examined their effectiveness or impact on decision-making.ConclusionThis scoping review describes how data from RTLS technology has been used to support clinical care of older adults with dementia. Research efforts have progressed from using the data to track activity levels to, most recently, using the data to inform clinical decision-making and as a predictor of delirium. Future studies are needed to validate RTLS-based health indices and examine how these indices can be used to inform decision-making.

The high host genetic background of tissue biopsies hinders the application of shotgun metagenomic sequencing in characterizing the tissue microbiota. We proposed an optimized method that removed host DNA from colon biopsies and examined the effect on metagenomic analysis. Human or mouse colon biopsies were divided into two groups, with one group undergoing host DNA depletion while the other serving as the control. Host DNAs were removed through differential lysis of mammalian and bacterial cells before sequencing. The impact of host DNA depletion on microbiota was compared based on phylogenetic diversity analyses and regression analyses. Removing host DNA enhanced bacterial sequencing depth and improved species discovery, increasing bacterial reads by 2.46 ± 0.20 fold while reducing host reads by 6.80% ± 1.06%. Moreover, 3.40 times more of bacterial species were detected after host DNA depletion. This was confirmed from mouse colon tissues, increasing bacterial reads by 5.46 ± 0.42 fold while decreasing host reads by 10.2% ± 0.83%. Similarly, significantly more species were detected in mouse colon tissue upon host DNA depletion (P0.001). Furthermore, an increased microbial richness was evident in the host DNA-depleted samples compared to non-depleted controls in human colon biopsies and mouse colon tissues (P0.001). Our optimized method of host DNA depletion improved the sensitivity of shotgun metagenomic sequencing in bacterial detection in the biopsy, which may yield a more accurate taxonomic profile of the tissue microbiota and identify bacteria that are important for disease initiation or progression.

Binder jetting (inkjet), featured by the dripping of liquid on the powder-based layer for solidification and bonding, is the second most popular 3D printing (3DP) technology for the construction industry. Therefore, an overview of this technology is necessary for both industry and academia. The paper discussed the most suitable materials for the construction industry which can be used in binder jetting 3DP. Attention was given not only to the selection of the materials but also the printing process and challenges that might face the printing process and post-processing stages, with an emphasis on sustainability and suitability. The whole process of printing gypsum, cement and geopolymer mortar, clay, chipped wood and sand materials through the binder jetting technique has been summarized, followed by curing and post-processing to achieve desirable mechanical properties. Finally, an informative approach was introduced for the scale-up of binder jetting 3DP in the construction industry.