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Background: Endothelial dysfunction has been suggested as a possible causal link between hyperglycemia and microvascular complications in diabetes mellitus. The effect of metformin on endothelial progenitor cells (EPCs) is still unclear. This study was designed to test the hypothesis that metformin could accelerate wound healing by improving the impaired EPC functions in streptozotocin-induced diabetic mice. Methods: Streptozotocin (STZ, 60 mg/kg/d × 5 d, i.p.) was injected to induce type 1 diabetes in male C57BL/6 mice. Mice were treated with metformin (250 mg/kg/d, i.g.) for consecutive 14 days. Wound closure was evaluated by wound area and number of CD31 stained capillaries. Functions of bone marrow-endothelial progenitor cells (BMEPCs) were assessed by tube formation and migration assays, and expression of AMP-activated protein kinase (AMPK) and endothelial nitric oxide synthase (eNOS) was determined by western blot analysis. Results: Metformin accelerated wound closure and stimulated angiogenesis in diabetic mice. The number of circulating EPCs was increased significantly in metformin treated diabetic mice. Abilities of tube formation and migration of BM-EPCs were impaired in diabetic mice, which were improved by metformin. Expression of both phosphorylated-AMPK and phosphorylated-eNOS was significantly increased, and nitric oxide (NO) production was enhanced by metformin in BM-EPCs of diabetic mice. In vitro, metformin improved impaired BM-EPC functions, and increased phosphorylated-eNOS expression and NO production in cultured BM-EPCs caused by high glucose, which was prevented by the AMPK inhibitor compound C. Conclusions: Our results suggest that metformin could improve BM-EPC functions in STZ-induced diabetic mice, which was possibly dependent on the AMPK/eNOS pathway. [ABSTRACT FROM AUTHOR]

Thepotential energy surface (PES) for reaction C2H4+ HO2was examined by using the quantum chemicalmethods. All rates were determined computationally using the CBS-QB3composite method combined with conventional transition state theory(TST),variational transition-state theory (VTST) and Rice–Ramsberger–Kassel–Marcus/master-equation(RRKM/ME) theory. The geometries optimization and the vibrationalfrequency analysis of reactants, transition states, and products wereperformed at the B3LYP/CBSB7 level. The composite CBS-QB3 method wasapplied for energy calculations. The major product channel of reactionC2H4+ HO2is the formation C2H4O2H via an OH···π complex with 3.7 kcal/mol binding energy which exhibits negative-temperaturedependence. We further investigated the reactions related to thiscomplex, which were ignored in previous studies. Thermochemical propertiesof the species involved in the reactions were determined using theCBS-QB3 method, and enthalpies of formation of species were comparedwith literature values. The calculated rate constants are in goodagreement with those available from literature and given in modifiedArrhenius equation form, which are serviceable in combustion modelingof hydrocarbons. Finally, in order to illustrate the effect for low-temperatureignition of our new rate constants, we have implemented them intothe existing mechanisms, which can predict ethylene ignition in ashock tube with better performance. [ABSTRACT FROM AUTHOR]

Sepsis is a common disease that continues to increase in incidence in the world. Diseases, such as diabetes, may make the situation worse. Diabetic patients are at increased risk for common infections. This study was designed to investigate the role of glibenclamide on myocardial injury by lipopolysaccharides (LPS) in streptozotocin induced diabetic mice (STZ-mice). Methods LPS was used to induce endotoxemia in STZ-mice. Heart rate and mean arterial pressure were measured by MPA-HBBS. Serum epinephrine level was measured by enzyme-linked immunosorbent assays (ELISA). Myocardial injury was examined by light and transmission electron microscope and TUNEL staining. Macrophage infiltration was measured by immunohistochemistry. Interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) levels in myocardial tissue and serum in STZ-mice, and in conditional medium of primary cultured peritoneal macrophages were determined by ELISA. Nalp3 and Caspase-1 protein levels were measured by Western blotting analysis. Results STZ administration decreased body weight and increased blood glucose in C57BL/6 mice. LPS injection caused decreases of heart rate and mean arterial pressure, and elevated serum epinephrine level in C57BL/6 mice. Compared with control mice without STZ treatment, LPS induced more severe myocardial injury and macrophage infiltration in STZ-mice, which was attenuated by pretreatment of glibenclamide. LPS stimulation enhanced the levels of IL- 1β and TNF-α in both cardiac tissue and serum. Glibenclamide pretreatment significantly inhibited the serum levels of pro-inflammatory cytokines. Either high glucose or LPS increased the levels of IL-1β and TNF-α in the conditional medium of peritoneal macrophages. Glibenclamide treatment suppressed the increase of IL-1β level induced by high glucose and LPS. Furthermore, Nalp3 and Caspase-1 levels were markedly increased by high glucose plus LPS, and both proteins were significantly inhibited by glibenclamide treatment. Conclusions We conclude that glibenclamide could attenuate myocardial injury induced by LPS challenge in STZ-mice, which was possibly related to inhibiting inflammation through Nalp3 inflammasomes. [ABSTRACT FROM AUTHOR]

Background: Cardiac dysfunction is well-described in endotoxemia and diagnosed in up to 60% of patients with endotoxic shock. ATP-sensitive potassium (KATP) channels are critical to cardiac function. This study investigates the role of Kir6.2 subunits of KATP channels on cardiac dysfunction in lipopolysaccharide (LPS)-induced endotoxemia. Methods: Kir6.2 subunits knockout (Kir6.2-/-) and wild-type (WT) mice were injected with LPS to induce endotoxemia. Cardiac function was monitored by echocardiography. Left ventricles were taken for microscopy (both light and electron) and TUNEL examination. Serum lactate dehydrogenase (LDH) and creatine kinase (CK) activities, and tumor necrosis factor-α (TNF-α) levels in both serum and left ventricular tissues were determined. Results: Compared to WT, Kir6.2-/- mice showed significantly declined cardiac function 360 min after LPS administration, aggravated myocardial damage and elevated serum LDH and CK activities. Apoptotic cells were obviously increased in heart tissues from Kir6.2-/- mice at 90, 180 and 360 min. TNF-α expression in both serum and heart tissues of Kir6.2-/- mice was significantly increased. Conclusions: We conclude that Kir6.2 subunits are critical in resistance to endotoxemia-induced cardiac dysfunction through reducing myocardial damage by inhibition of apoptosis and inflammation. KATP channels blockers are extensively used in the treatment of diabetes, their potential role should therefore be considered in the clinic when patients treated with antidiabetic sulfonylureas are complicated by endotoxemia. [ABSTRACT FROM AUTHOR]

C6H6(SiF2)3 has a very unique spatial geometry shape, electronic structure, and hetero‐chain structure. The addition of external electric field makes C6H6(SiF2)3 become a strong candidate for the research and development of new functional materials. Based on quantum chemical calculation, we studied the influence of external electric field on C6H6(SiF2)3 and came to the conclusion that the addition of external electric field makes the molecular structure of C6H6(SiF2)3 occur obvious deformation. The essence of structural deformation is energy change and atomic force. But if the external electric field is removed, C6H6(SiF2)3 will return to its original configuration; that is, C6H6(SiF2)3 has obvious elastic characteristics. The existence of strong external electric field will lead to the charge polarization distribution of C6H6(SiF2)3. With the increase of external electric field intensity, C6H6(SiF2)3 shows color in the visible light range, and it can be controlled to show different color by changing the intensity of external electric field. In addition, applying external electric field to C6H6(SiF2)3 in invisible solvent benzene can save more electricity than that without solvent, and the absorption band in the visible light range is more significant. Therefore, invisible solvent can be added to enhance its color display under different external electric field intensity. [ABSTRACT FROM AUTHOR]

In this paper, we construct a Si x F y ( x ≤ 6, y ≤ 12) series optimised at the B3LYP/6-31G(d,p) level. At the same level, we perform frontline molecular orbital (FMO), Mayer bond order (MBO), molecular surface electrostatic potential (MS-EPS) and natural population analysis (NPA) calculations to study the chemical structure stabilities of these Si x F y molecules. The FMO and MBO results demonstrate that the chemical structure stabilities of the Si x F y ( x ≤ 6, y ≤ 12) series are ranked (from strong to weak) as SiF 4 > Si 2 F 6 > Si 3 F 8 > Si 4 F 10 > SiF 2 > Si 5 F 12 > Si 3 F 6 (ring) > Si 5 F 10 (ring) > Si 6 F 12 (ring) > Si 4 F 8 (ring). Furthermore, the chemical structure stabilities of the chains are stronger than those of the rings, while the number of silicon atoms is the same. In addition, infrared spectroscopy analysis shows that SiF 4 is the most stable among the Si x F y ( x ≤ 6, y ≤ 12) series, followed by Si 2 F 6 , and SiF 2 is unstable. The experimental results are consistent with theoretical calculations. Finally, the MS-EPS and NPA results indicate that compounds in the Si x F y ( x ≤ 6, y ≤ 12) series tend to be attacked by nucleophiles rather than by electrophiles; also, they show poor chemical structure stability when encountering nucleophiles., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)