332 results on '"Guillaume N"'
Search Results
52. Profiling of immune related genes silenced in EBV-positive gastric carcinoma identified novel restriction factors of human gammaherpesviruses
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Fiches, Guillaume N., primary, Zhou, Dawei, additional, Kong, Weili, additional, Biswas, Ayan, additional, Ahmed, Elshafa H., additional, Baiocchi, Robert A., additional, Zhu, Jian, additional, and Santoso, Netty, additional
- Published
- 2020
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53. Agglomeration of Viruses by Cationic Lignin Particles for Facilitated Water Purification
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Rivière, Guillaume N., primary, Korpi, Antti, additional, Sipponen, Mika Henrikki, additional, Zou, Tao, additional, Kostiainen, Mauri A., additional, and Österberg, Monika, additional
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- 2020
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54. Lung immune signatures define two groups of end-stage IPF patients
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Tamara Cruz, Núria Mendoza, Sandra Casas-Recasens, Guillaume Noell, Fernanda Hernandez-Gonzalez, Alejandro Frino-Garcia, Xavi Alsina-Restoy, María Molina, Mauricio Rojas, Alvar Agustí, Jacobo Sellares, and Rosa Faner
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Immune-signatures ,Transcriptome ,Idiopathic pulmonary fibrosis ,Flow cytometry ,Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background The role of the immune system in the pathobiology of Idiopathic Pulmonary Fibrosis (IPF) is controversial. Methods To investigate it, we calculated immune signatures with Gene Set Variation Analysis (GSVA) and applied them to the lung transcriptome followed by unbiased cluster analysis of GSVA immune-enrichment scores, in 109 IPF patients from the Lung Tissue Research Consortium (LTRC). Results were validated experimentally using cell-based methods (flow cytometry) in lung tissue of IPF patients from the University of Pittsburgh (n = 26). Finally, differential gene expression and hypergeometric test were used to explore non-immune differences between clusters. Results We identified two clusters (C#1 and C#2) of IPF patients of similar size in the LTRC dataset. C#1 included 58 patients (53%) with enrichment in GSVA immune signatures, particularly cytotoxic and memory T cells signatures, whereas C#2 included 51 patients (47%) with an overall lower expression of GSVA immune signatures (results were validated by flow cytometry with similar unbiased clustering generation). Differential gene expression between clusters identified differences in cilium, epithelial and secretory cell genes, all of them showing an inverse correlation with the immune response signatures. Notably, both clusters showed distinct features despite clinical similarities. Conclusions In end-stage IPF lung tissue, we identified two clusters of patients with very different levels of immune signatures and gene expression but with similar clinical characteristics. Weather these immune clusters differentiate diverse disease trajectories remains unexplored.
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- 2023
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55. Complications neurologiques et vaccination contre l'hépatite B : l'impossible conciliation entre la preuve scientifique et la preuve judiciaire. Conséquences sur la pratique expertale
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Rougé-Maillart, Cl., Jousset, N., Guillaume, N., and Penneau, M.
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- 2005
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56. Study and characterization of the irreversible transformation of electrically stressed planar Ti/TiOx/Ti junctions.
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Guillaume, N., Puyoo, E., Le Berre, M., Albertini, D., Baboux, N., Chevalier, C., Ayadi, K., Grégoire, J., Gautier, B., and Calmon, F.
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TUNNEL junctions (Materials science) , *ELECTROLYTIC oxidation , *PLANAR antennas , *RUTILE , *PLANAR chirality , *ATOMIC force microscopy - Abstract
We investigate the properties and characteristics of planar Ti/TiOx/Ti junctions, which consist of transverse TiOx lines drawn on Ti test patterns. Junctions are elaborated by means of local anodic oxidation using atomic force microscopy. An irreversible morphological transformation occurring in a reproducible manner is observed when these planar junctions are electrically stressed under ambient atmosphere. Structural and chemical analyses based on transmission electron microscopy techniques reveal the extension of the initial amorphous TiOx into a crystalline rutile phase. This irreversible transformation is proven to vanish completely if the electrical stress occurs under vacuum atmosphere. Finally, we carry out temperature dependent electrical measurements in order to elucidate their conduction mechanism: Schottky emission above an ultra-low potential barrier is assumed to dominate under vacuum atmosphere whereas ionic conduction seems to prevail in air. [ABSTRACT FROM AUTHOR]
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- 2015
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57. Associations between load-velocity profiling and race parameters of elite swimmers in the 100 and 200m freestyle events
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Yannis Raineteau, Guillaume Nicolas, Benoit Bideau, Nicolas Bideau, and Robin Pla
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swimming technique ,propulsion ,stroke regulation ,load-velocity profiles ,competitive swimming ,Sports ,GV557-1198.995 - Abstract
IntroductionImproving swimming performance involves assessments of biomechanical variables of the stroke, and it can be achieved using semi-tethered swimming tests. The aim of this study was thus to investigate the associations between load-velocity (L-V) profiles, from a semi-tethered swimming protocol and race variables in the 100 m and 200 m freestyle events.MethodsEight swimmers completed a L-V profiling protocol consisting of four sprints (25 m, 25 m, 20 m, 15 m) against increasing loads (0.1, 2.0, 4.0, 6.0 kg respectively) with complete recovery between repetitions (>5 min). The L-V linear regression was used to estimate maximal velocity (V0) and body mass normalized load (rL0). Race variables such as clean swimming speed (V), stroke rate (SR), distance per cycle (SL) and stroke index (SI) were assessed from video analysis of 100 m and 200 m freestyle events taking place 3–4 days after the L-V protocol.ResultsL-V results showed high levels of speed (mean ± SD: 1.87 ± 0.04 m/s) and heavy maximal relative loads (mean ± SD: 38.5 ± 6.51 as % of body mass). Swimmers also achieved high-level performances in the 100 m (mean ± SD time: 51.95 ± 0.75 s) and the 200 m (mean ± SD time: 113.85 ± 2.67 s). For the 100 m events, the maximal relative load showed strong correlation with performance (r = 0.63) whereas trivial correlation was observed for the 200 m events (r = 0.12). SR on the 100 m and the 200 m also showed very strong association with rL0 (r = 0.83) and a strong association with V0 (r = 0.68) respectively.ConclusionThe relationships between L-V variables and race variables depend on the distance of the event. However, L-V variables seem to be less related to SR and SL evolutions for the 100 m than in the 200 m event. Moreover, L-V profiles tend to be more related to the 100 m than 200 m freestyle performance. L-V profile should be interpreted taking into consideration the specific physiological and biomechanical constraints of the main events of the swimmer.
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- 2023
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58. Innovative carbon-nitrogen-TiO2 catalyst immobilised on stainless steel mesh for effective removal of orange II organic dye in the DCDBD plasma actuator
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Emile Salomon Massima Mouele, Jean-Luc Mukaba, Guillaume Ndayambaje, Jimoh Oladejo Tijani, Chuks Paul Eze, Evral Ntsa, Myo Tay Zar Myint, Htet Htet Kyaw, Mohammed Al-Abri, Sergey Dobretsov, Bernard Bladergroen, and Leslie Petrik
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Wastewater ,Photocatalysts ,O.II dye ,DCDBD ,Degradation ,Advanced technology ,Chemistry ,QD1-999 - Abstract
A double cylindrical dielectric barrier discharge (DCDBD) was optimised and coupled with SS/C-N-TiO2 photocatalysts produced by sol gel technique for the treatment of orange II (O.II) dye. The results show that degradation of 60 mg/L O.II with both systems followed first-order reaction. Higher removal of O.II was achieved with DCDBD-SS/C-N-TiO2 technology. The decomposition of O.II resulted in six and four degradation metabolites with DCDBD alone, and DCDBD-SS/C-N-TiO2, respectively. The COD and TOC removals reached 61.43% and 15.38%, respectively after 60 min of DCDBD run. This study demonstrates that DCDBD is a promising advanced technology for the treatment of dye wastewater.
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- 2023
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59. Improvement of HRS Variability in OxRRAM by Tailored Metallic Liner
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Guillaume, N., primary, Azzaz, M., additional, Blonkowski, S., additional, Jalaguier, E., additional, Gonon, P., additional, Vallée, C., additional, Blomberg, T., additional, Tuominen, M., additional, Sprey, H., additional, Bernasconi, S., additional, Charpin-Nicolle, C., additional, and Nowak, E., additional
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- 2019
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60. Antimicrobial Colloidal Silver–Lignin Particles via Ion and Solvent Exchange
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Lintinen, Kalle, primary, Luiro, Sanna, additional, Figueiredo, Patrícia, additional, Sakarinen, Ekaterina, additional, Mousavi, Zekra, additional, Seitsonen, Jani, additional, Rivière, Guillaume N. S., additional, Mattinen, Ulriika, additional, Niemelä, Matti, additional, Tammela, Päivi, additional, Österberg, Monika, additional, Johansson, Leena-Sisko, additional, Bobacka, Johan, additional, Santos, Hélder A., additional, and Kostiainen, Mauri A., additional
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- 2019
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61. Germline RUNX1 variants in paediatric patients in a French specialised centre
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Cécile Liu, Paola Ballerini, Guillaume Nguyen, Zoia Mincheva, Bruno Copin, Boutheina Bouslama, Guy Leverger, Arnaud Petit, Rémi Favier, Hélène Lapillonne, and Hélène Boutroux
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genetic predisposition to disease ,haematologic diseases ,RUNX1 ,Diseases of the blood and blood-forming organs ,RC633-647.5 - Abstract
Abstract Familial platelet disorder with associated myeloid malignancy (FPD‐MM; OMIM 601399) is related to germline RUNX1 mutation. The pathogenicity of RUNX1 variants was initially linked to FPD‐MM phenotype, but the discovery of new variants through the expansion of genetic explorations in leukaemia is questioning this assertion. In this study, we add 10 families with germline RUNX1 variant explored at Armand Trousseau Hospital for leukaemia diagnosis or thrombocytopenia, to the 259 described so far. Detailed description of their personal and family history of haematological pathologies allows identifying three phenotypes related to germline RUNX1 variants: thrombocytopenia and/or malignant haematological disease with family history of haematological diseases, thrombocytopenia with no family history of haematological diseases and acute lymphoblastic leukaemia (ALL) with no family history of haematological diseases. In the latter phenotype, ALL characteristics involving RUNX1 suggest the implication of germline variants in the onset of the malignancy.
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- 2023
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62. Poliovirus-Neutralizing Antibody Seroprevalence and Vaccine Habits in a Vaccine-Derived Poliovirus Outbreak Region in the Democratic Republic of Congo in 2018: The Impact on the Global Eradication Initiative
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Megan Halbrook, Adva Gadoth, Patrick Mukadi, Nicole A. Hoff, Kamy Musene, Camille Dzogang, Cyrus Shannon Sinai, D’Andre Spencer, Guillaume Ngoie-Mwamba, Sylvia Tangney, Frank Salet, Michel Nyembwe, Michel Kambamba Nzaji, Merly Tambu, Placide Mbala, Trevon Fuller, Sue K. Gerber, Didine Kaba, Jean Jacques Muyembe-Tamfum, and Anne W. Rimoin
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poliovirus ,serosurvey ,Democratic Republic of the Congo ,vaccine coverage ,cVDPV ,SIAs ,Medicine - Abstract
Despite the successes in wild-type polio eradication, poor vaccine coverage in the DRC has led to the occurrence of circulating vaccine-derived poliovirus outbreaks. This cross-sectional population-based survey provides an update to previous poliovirus-neutralizing antibody seroprevalence studies in the DRC and quantifies risk factors for under-immunization and parental knowledge that guide vaccine decision making. Among the 964 children between 6 and 35 months in our survey, 43.8% (95% CI: 40.6–47.0%), 41.1% (38.0–44.2%), and 38.0% (34.9–41.0%) had protective neutralizing titers to polio types 1, 2, and 3, respectively. We found that 60.7% of parents reported knowing about polio, yet 25.6% reported knowing how it spreads. Our data supported the conclusion that polio outreach efforts were successfully connecting with communities—79.4% of participants had someone come to their home with information about polio, and 88.5% had heard of a polio vaccination campaign. Additionally, the odds of seroreactivity to only serotype 2 were far greater in health zones that had a history of supplementary immunization activities (SIAs) compared to health zones that did not. While SIAs may be reaching under-vaccinated communities as a whole, these results are a continuation of the downward trend of seroprevalence rates in this region.
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- 2024
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63. Surface contamination rates at different spatial scales in the Columbus module (ISS) during the MATISS campaigns
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Laurence Lemelle, Eléonore Mottin, Denis Le Tourneau, Sébastien Rouquette, Lucie Campagnolo, Cécile Thévenot, Alain Maillet, Sébastien Barde, Emmanuel Garre, Jérémie Teisseire, Caroline Fontelaye, Vincent Jousseaume, Catherine Pudda, Olivier Constantin, Pierre Marcoux, Guillaume Nonglaton, and Christophe Place
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life support ,biomaterials ,space applications ,space habitation ,earth applications ,astrobiology ,Astronomy ,QB1-991 ,Geophysics. Cosmic physics ,QC801-809 - Abstract
Future long-duration human spaceflights require developments to limit biocontamination of surface habitats. The three MATISS (Microbial Aerosol Tethering on Innovative Surfaces in the International Space Station) campaigns exposed surface treatments over several months in the ISS. To this end, eight sample holders designed were mounted with lamella-bearing FDTS ((1H, 1H, 2H, 2H)-perfluorodecyltrichlorosilane), SiOCH, and parylene hydrophobic coatings, at two different locations, for several months, during three distinct periods from 2016 to 2020. Tile scanning optical microscopy (×3 and ×30 magnifications) detected several thousand particles, indicating a relatively clean environment (a few particles per mm2). In previous studies, exposure rates were analyzed for all the coarse and fine particles detected on the largest total area of the integrated FDTS area exposed in the ISS (several cm2). Here, the contamination rates observed for a smaller constant area unit (the 0.66-cm2 window area of the holder) were statistically analyzed. Therefore, a statistical difference in rate distributions between RGSH (Return Grid Sensor House) and EDR (European Drawer Rack) and between FDTS and either SiOCH or parylene was shown for the coarse particles but not for the fine particles. The contamination rates were found to be low, confirming the efficiency of the long-term air purification system. The rates tend to vary with the astronaut occupancy rates. Surfaces of spacecraft for long-duration exploration left unmanned during dormancy periods can be considered safe from biocontamination.
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- 2023
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64. Similarity network fusion to identify phenotypes of small-for-gestational-age fetuses
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Jezid Miranda, Cristina Paules, Guillaume Noell, Lina Youssef, Angel Paternina-Caicedo, Francesca Crovetto, Nicolau Cañellas, María L. Garcia-Martín, Nuria Amigó, Elisenda Eixarch, Rosa Faner, Francesc Figueras, Rui V. Simões, Fàtima Crispi, and Eduard Gratacós
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Public health ,Pregnancy ,Machine learning ,Science - Abstract
Summary: Fetal growth restriction (FGR) affects 5–10% of pregnancies, is the largest contributor to fetal death, and can have long-term consequences for the child. Implementation of a standard clinical classification system is hampered by the multiphenotypic spectrum of small fetuses with substantial differences in perinatal risks. Machine learning and multiomics data can potentially revolutionize clinical decision-making in FGR by identifying new phenotypes. Herein, we describe a cluster analysis of FGR based on an unbiased machine-learning method. Our results confirm the existence of two subtypes of human FGR with distinct molecular and clinical features based on multiomic analysis. In addition, we demonstrated that clusters generated by machine learning significantly outperform single data subtype analysis and biologically support the current clinical classification in predicting adverse maternal and neonatal outcomes. Our approach can aid in the refinement of clinical classification systems for FGR supported by molecular and clinical signatures.
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- 2023
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65. Interdisciplinary Swiss consensus recommendations on staging and treatment of advanced prostate cancer
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Arnoud J. Templeton, Aurelius Omlin, Dominik Berthold, Jörg Beyer, Irene A. Burger, Daniel Eberli, Daniel Engeler, Christian Fankhauser, Stefanie Fischer, Silke Gillessen, Guillaume Nicolas, Stephanie Kroeze, Anja Lorch, Michael Müntener, Alexandros Papachristofilou, Niklaus Schaefer, Daniel Seiler, Frank Stenner, Petros Tsantoulis, Tatjana Vlajnic, Thomas Zilli, Daniel Zwahlen, and Richard Cathomas
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Medicine - Abstract
The management of prostate cancer is undergoing rapid changes in all disease settings. Novel imaging tools for diagnosis have been introduced, and the treatment of high-risk localized, locally advanced and metastatic disease has changed considerably in recent years. From clinical and health-economic perspectives, a rational and optimal use of the available options is of the utmost importance. While international guidelines list relevant pivotal trials and give recommendations for a variety of clinical scenarios, there is much room for interpretation, and several important questions remain highly debated. The goal of developing a national consensus on the use of these novel diagnostic and therapeutic strategies in order to improve disease management and eventually patient outcomes has prompted a Swiss consensus meeting. Experts from several specialties, including urology, medical oncology, radiation oncology, pathology and nuclear medicine, discussed and voted on questions of the current most important areas of uncertainty, including the staging and treatment of high-risk localized disease, treatment of metastatic hormone-sensitive prostate cancer (mHSPC) and use of new options to treat metastatic castration-resistant prostate cancer (mCRPC).
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- 2023
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66. Dry side of the core: a meta-analysis addressing the original nature of the ABA signalosome at the onset of seed imbibition
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Guillaume Née and Thorben Krüger
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abscisic acid (ABA) ,seed germination ,protein post-translational modifications (PTMs) ,molecular signalling ,proteomics ,Plant culture ,SB1-1110 - Abstract
The timing of seedling emergence is a major agricultural and ecological fitness trait, and seed germination is controlled by a complex molecular network including phytohormone signalling. One such phytohormone, abscisic acid (ABA), controls a large array of stress and developmental processes, and researchers have long known it plays a crucial role in repressing germination. Although the main molecular components of the ABA signalling pathway have now been identified, the molecular mechanisms through which ABA elicits specific responses in distinct organs is still enigmatic. To address the fundamental characteristics of ABA signalling during germination, we performed a meta-analysis focusing on the Arabidopsis dry seed proteome as a reflexion basis. We combined cutting-edge proteome studies, comparative functional analyses, and protein interaction information with genetic and physiological data to redefine the singular composition and operation of the ABA core signalosome from the onset of seed imbibition. In addition, we performed a literature survey to integrate peripheral regulators present in seeds that directly regulate core component function. Although this may only be the tip of the iceberg, this extended model of ABA signalling in seeds already depicts a highly flexible system able to integrate a multitude of information to fine-tune the progression of germination.
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- 2023
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67. MICA AND NKG2D POLYMORPHISMS HAVE A SIGNIFICANT IMPACT ON GRAFT VERSUS HOST DISEASE AFTER HLA-MATCHED HEMATOPOIETIC STEM CELL TRANSPLANTATION
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Apithy, M-J, Charbonnier, A., Desoutter, J., Morel, P., Marolleau, J-P, Guillaume, N., and DESSAIVRE, Louise
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[SDV] Life Sciences [q-bio] - Published
- 2017
68. Dynamic Imaging of the Hepatitis C Virus NS5A Protein during a Productive Infection
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Amanda L. Aloia, Guillaume N. Fiches, Nicholas S. Eyre, Kui Li, Christopher S. P. McErlean, Erin M. McCartney, Anupriya Aggarwal, Michael R. Beard, Stuart Turville, and Karla J. Helbig
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viruses ,Hepatitis C virus ,Hepacivirus ,Immunology ,Vesicular Transport Proteins ,Viral Nonstructural Proteins ,Virus Replication ,medicine.disease_cause ,Microtubules ,Microbiology ,Virus ,Cell Line ,Lipid droplet ,Virology ,medicine ,Humans ,NS5A ,rab5 GTP-Binding Proteins ,biology ,Virus Assembly ,Dyneins ,virus diseases ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,digestive system diseases ,Virus-Cell Interactions ,Cell biology ,NS2-3 protease ,Viral replication ,Insect Science ,Hepatocytes ,Viral genome replication - Abstract
Hepatitis C virus (HCV) NS5A is essential for viral genome replication within cytoplasmic replication complexes and virus assembly at the lipid droplet (LD) surface, although its definitive functions are poorly understood. We developed approaches to investigate NS5A dynamics during a productive infection. We report here that NS5A motility and efficient HCV RNA replication require the microtubule network and the cytoplasmic motor dynein and demonstrate that both motile and relatively static NS5A-positive foci are enriched with host factors VAP-A and Rab5A. Pulse-chase imaging revealed that newly synthesized NS5A foci are small and distinct from aged foci, while further studies using a unique dual fluorescently tagged infectious HCV chimera showed a relatively stable association of NS5A foci with core-capped LDs. These results reveal new details about the dynamics and maturation of NS5A and the nature of potential sites of convergence of HCV replication and assembly pathways. IMPORTANCE Hepatitis C virus (HCV) is a major cause of serious liver disease worldwide. An improved understanding of the HCV replication cycle will enable development of novel and improved antiviral strategies. Here we have developed complementary fluorescent labeling and imaging approaches to investigate the localization, traffic and interactions of the HCV NS5A protein in living, virus-producing cells. These studies reveal new details as to the traffic, composition and biogenesis of NS5A foci and the nature of their association with putative sites of virus assembly.
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- 2014
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69. Passive limitation of surface contamination by perFluoroDecylTrichloroSilane coatings in the ISS during the MATISS experiments
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Laurence Lemelle, Sébastien Rouquette, Eléonore Mottin, Denis Le Tourneau, Pierre R. Marcoux, Cécile Thévenot, Alain Maillet, Guillaume Nonglaton, and Christophe Place
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Biotechnology ,TP248.13-248.65 ,Physiology ,QP1-981 - Abstract
Abstract Future long-duration human spaceflight will require developments to limit biocontamination of surface habitats. The MATISS (Microbial Aerosol Tethering on Innovative Surfaces in the international Space Station) experiments allowed for exposing surface treatments in the ISS (International Space Station) using a sample-holder developed to this end. Three campaigns of FDTS (perFluoroDecylTrichloroSilane) surface exposures were performed over monthly durations during distinct periods. Tile scanning optical microscopy (×3 and ×30 magnifications) showed a relatively clean environment with a few particles on the surface (0.8 to 7 particles per mm2). The varied densities and shapes in the coarse area fraction (50–1500 µm2) indicated different sources of contamination in the long term, while the bacteriomorph shapes of the fine area fraction (0.5–15 µm2) were consistent with microbial contamination. The surface contamination rates correlate to astronauts’ occupancy rates on board. Asymmetric particles density profiles formed throughout time along the air-flow. The higher density values were located near the flow entry for the coarse particles, while the opposite was the case for the fine particles, probably indicating the hydrophobic interaction of particles with the FDTS surface.
- Published
- 2022
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70. Thioredoxins m regulate plastid glucose-6-phosphate dehydrogenase activity in Arabidopsis roots under salt stress
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Guillaume Née, Fuzheng Wang, Gilles Châtel-Innocenti, Amna Mhamdi, Eugénie Juranville, Hélène Vanacker, Graham Noctor, and Emmanuelle Issakidis-Bourguet
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glucose-6-phosphate dehydrogenase ,redox regulation ,Arabidopsis root ,salt stress ,plastid thioredoxins ,Plant culture ,SB1-1110 - Abstract
Plants contain several NADPH-producing enzymes including glucose-6-phosphate dehydrogenases (G6PDH) with different sub-cellular localizations. The activity of plastidial G6PDHs is redox-regulated by thioredoxins (TRX). Although specific TRXs are known to regulate chloroplastic isoforms of G6PDH, little information is available for plastidic isoforms found in heterotrophic organs or tissues. Here, we investigated TRX regulation of the two G6PDH plastidic isoforms of Arabidopsis roots during exposure to a mild salt stress. We report that in vitro m-type TRXs are the most efficient regulators of the G6PDH2 and G6PDH3 mainly found in Arabidopsis roots. While expression of the corresponding G6PD and plastidic TRX genes was marginally affected by salt, it impaired root growth of several of the corresponding mutant lines. Using an in situ assay for G6PDH, G6PDH2 was found to be the major contributor to salt-induced increases in activity, while data from ROS assays further provide in vivo evidence that TRX m acts in redox regulation during salt stress. Taken together, our data suggest that regulation of plastid G6PDH activity by TRX m may be an important player regulating NADPH production in Arabidopsis roots undergoing salt stress.
- Published
- 2023
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71. HCV RNA traffic and association with NS5A in living cells
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Nicholas S. Eyre, Abha Chopra, Guillaume N. Fiches, Brigit Betz-Stablein, Amanda L. Aloia, Michael R. Beard, Kylie H. Van der Hoek, and Fabio Luciani
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0301 basic medicine ,Untranslated region ,Cytoplasm ,viruses ,Hepatitis C virus ,Hepacivirus ,Viral Nonstructural Proteins ,medicine.disease_cause ,Virus Replication ,Genome ,Cell Line ,03 medical and health sciences ,Virology ,medicine ,NS5A ,biology ,Inverted Repeat Sequences ,virus diseases ,RNA ,biology.organism_classification ,Fusion protein ,digestive system diseases ,030104 developmental biology ,Viral replication ,RNA, Viral - Abstract
The spatiotemporal dynamics of Hepatitis C Virus (HCV) RNA localisation are poorly understood. To address this we engineered HCV genomes harbouring MS2 bacteriophage RNA stem-loops within the 3'-untranslated region to allow tracking of HCV RNA via specific interaction with a MS2-Coat-mCherry fusion protein. Despite the impact of these insertions on viral fitness, live imaging revealed that replication of tagged-HCV genomes induced specific redistribution of the mCherry-tagged-MS2-Coat protein to motile and static foci. Further analysis showed that HCV RNA was associated with NS5A in both static and motile structures while a subset of motile NS5A structures was devoid of HCV RNA. Further investigation of viral RNA traffic with respect to lipid droplets (LDs) revealed HCV RNA-positive structures in close association with LDs. These studies provide new insights into the dynamics of HCV RNA traffic with NS5A and LDs and provide a platform for future investigations of HCV replication and assembly.
- Published
- 2015
72. The antiviral protein viperin inhibits hepatitis C virus replication via interaction with nonstructural protein 5A
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Sumudu K. Narayana, Nicholas S. Eyre, Stanley M. Lemon, Rohit K. Jangra, Michael R. Beard, Evelyn Yip, Guillaume N. Fiches, Kui Li, Karla J. Helbig, and Erin M. McCartney
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Oxidoreductases Acting on CH-CH Group Donors ,Carcinoma, Hepatocellular ,viruses ,Vesicular Transport Proteins ,Antiviral protein ,Alpha interferon ,Hepacivirus ,Viral Nonstructural Proteins ,Biology ,Virus Replication ,Article ,Viral life cycle ,Interferon ,Cell Line, Tumor ,medicine ,Humans ,RNA, Small Interfering ,NS5A ,Host factor ,Hepatology ,Liver Neoplasms ,Interferon-alpha ,Proteins ,virus diseases ,Hepatitis C, Chronic ,Virology ,digestive system diseases ,Viral replication ,Mutagenesis ,Viperin ,medicine.drug - Abstract
The interferon-stimulated gene, viperin, has been shown to have antiviral activity against hepatitis C virus (HCV) in the context of the HCV replicon, although the molecular mechanisms responsible are not well understood. Here, we demonstrate that viperin plays an integral part in the ability of interferon to limit the replication of cell-culture–derived HCV (JFH-1) that accurately reflects the complete viral life cycle. Using confocal microscopy and fluorescence resonance energy transfer (FRET) analysis, we demonstrate that viperin localizes and interacts with HCV nonstructural protein 5A (NS5A) at the lipid-droplet (LD) interface. In addition, viperin also associates with NS5A and the proviral cellular factor, human vesicle-associated membrane protein-associated protein subtype A (VAP-A), at the HCV replication complex. The ability of viperin to limit HCV replication was dependent on residues within the C-terminus, as well as an N-terminal amphipathic helix. Removal of the amphipathic helix-redirected viperin from the cytosolic face of the endoplasmic reticulum and the LD to a homogenous cytoplasmic distribution, coinciding with a loss of antiviral effect. C-terminal viperin mutants still localized to the LD interface and replication complexes, but did not interact with NS5A proteins, as determined by FRET analysis. Conclusion: In conclusion, we propose that viperin interacts with NS5A and the host factor, VAP-A, to limit HCV replication at the replication complex. This highlights the complexity of the host control of viral replication by interferon-stimulated gene expression. (HEPATOLOGY 2011;)
- Published
- 2011
73. Characterization of NRPS and PKS genes involved in the biosynthesis of SMs in Alternaria dauci including the phytotoxic polyketide aldaulactone
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Julia Courtial, Jean-Jacques Helesbeux, Hugo Oudart, Sophie Aligon, Muriel Bahut, Bruno Hamon, Guillaume N’Guyen, Sandrine Pigné, Ahmed G. Hussain, Claire Pascouau, Nelly Bataillé-Simoneau, Jérôme Collemare, Romain Berruyer, and Pascal Poupard
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Medicine ,Science - Abstract
Abstract Alternaria dauci is a Dothideomycete fungus, causal agent of carrot leaf blight. As a member of the Alternaria genus, known to produce a lot of secondary metabolite toxins, A. dauci is also supposed to synthetize host specific and non-host specific toxins playing a crucial role in pathogenicity. This study provides the first reviewing of secondary metabolism genetic basis in the Alternaria genus by prediction of 55 different putative core genes. Interestingly, aldaulactone, a phytotoxic benzenediol lactone from A. dauci, was demonstrated as important in pathogenicity and in carrot partial resistance to this fungus. As nothing is known about aldaulactone biosynthesis, bioinformatic analyses on a publicly available A. dauci genome data set that were reassembled, thanks to a transcriptome data set described here, allowed to identify 19 putative secondary metabolism clusters. We exploited phylogeny to pinpoint cluster 8 as a candidate in aldaulactone biosynthesis. This cluster contains AdPKS7 and AdPKS8, homologs with genes encoding a reducing and a non-reducing polyketide synthase. Clusters containing such a pair of PKS genes have been identified in the biosynthesis of resorcylic acid lactones or dihydroxyphenylacetic acid lactones. AdPKS7 and AdPKS8 gene expression patterns correlated with aldaulactone production in different experimental conditions. The present results highly suggest that both genes are responsible for aldaulactone biosynthesis.
- Published
- 2022
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74. Le polymorphisme VEGF 936 C>T est-il associé à un effet protecteur vis-à-vis du risque de carcinome à cellules rénales post-transplantation ?
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Bodeau, S., primary, Quinton, M.C., additional, Sauzay, C., additional, Bennis, Y., additional, Etienne, I., additional, Romezin, J., additional, Meynier, J., additional, Guillaume, N., additional, Hazzan, M., additional, Galmiche, A., additional, and Choukroun, G., additional
- Published
- 2017
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75. Severe neurological disorders and refractory aspergillosis in an adolescent treated by vincristine and voriconazole
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Bennis, Y., primary, Bodeau, S., additional, Lutun, A., additional, Gourmel, A., additional, Solas, C., additional, Quaranta, S., additional, Guillaume, N., additional, Chouaki, T., additional, Lemaire‐Hurtel, A.‐S., additional, and Masmoudi, K., additional
- Published
- 2017
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76. Viperin is induced following dengue virus type-2 (DENV-2) infection and has anti-viral actions requiring the C-terminal end of viperin
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Julie K. Calvert, Guillaume N. Fiches, Sumudu K. Narayana, Karla J. Helbig, Jillian M. Carr, Michael R. Beard, Erin M. McCartney, Satiya Wati, Nicholas S. Eyre, Jennifer N. Clarke, Helbig, Karla J, Carr, Jillian M, Calvert, Julie K, Wati, Satiya, Clarke, Jennifer N, Eyre, Nicholas S, Narayana, Sumudu K, Fiches, Guillaume N, McCartney, Erin M, and Beard, Michael R
- Subjects
Viral Diseases ,viruses ,fluorescence resonance energy transfer ,Dengue virus ,medicine.disease_cause ,Dengue Fever ,Small hairpin RNA ,Dengue ,lipid structure ,Chlorocebus aethiops ,reverse transcriptase-polymerase chain reaction ,0303 health sciences ,Reverse Transcriptase Polymerase Chain Reaction ,lcsh:Public aspects of medicine ,030302 biochemistry & molecular biology ,virus diseases ,Innate Immunity ,3. Good health ,Infectious Diseases ,Viperin ,Medicine ,Research Article ,Neglected Tropical Diseases ,Leucine zipper ,Oxidoreductases Acting on CH-CH Group Donors ,RNA virus ,lcsh:Arctic medicine. Tropical medicine ,lcsh:RC955-962 ,Blotting, Western ,Biology ,Microbiology ,lipids ,03 medical and health sciences ,Tropical Medicine ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Vero Cells ,030304 developmental biology ,NS3 ,dengue virus ,Interferon-stimulated gene ,Public Health, Environmental and Occupational Health ,Immunity ,RNA ,Proteins ,lcsh:RA1-1270 ,Dengue Virus ,biochemical phenomena, metabolism, and nutrition ,Virology ,RNA extraction ,Viral replication ,viral replication ,Parasitology - Abstract
The host protein viperin is an interferon stimulated gene (ISG) that is up-regulated during a number of viral infections. In this study we have shown that dengue virus type-2 (DENV-2) infection significantly induced viperin, co-incident with production of viral RNA and via a mechanism requiring retinoic acid-inducible gene I (RIG-I). Viperin did not inhibit DENV-2 entry but DENV-2 RNA and infectious virus release was inhibited in viperin expressing cells. Conversely, DENV-2 replicated to higher tires earlier in viperin shRNA expressing cells. The anti-DENV effect of viperin was mediated by residues within the C-terminal 17 amino acids of viperin and did not require the N-terminal residues, including the helix domain, leucine zipper and S-adenosylmethionine (SAM) motifs known to be involved in viperin intracellular membrane association. Viperin showed co-localisation with lipid droplet markers, and was co-localised and interacted with DENV-2 capsid (CA), NS3 and viral RNA. The ability of viperin to interact with DENV-2 NS3 was associated with its anti-viral activity, while co-localisation of viperin with lipid droplets was not. Thus, DENV-2 infection induces viperin which has anti-viral properties residing in the C-terminal region of the protein that act to restrict early DENV-2 RNA production/accumulation, potentially via interaction of viperin with DENV-2 NS3 and replication complexes. These anti-DENV-2 actions of viperin show both contrasts and similarities with other described anti-viral mechanisms of viperin action and highlight the diverse nature of this unique anti-viral host protein., Author Summary Viperin is a virally induced host protein that has been previously shown to have antiviral activity against a variety of viruses. Here we have demonstrated that viperin is also anti-viral against the medically significant arbovirus, dengue virus. Viperin was able to inhibit dengue virus at the level of viral replication, and cell lines unable to produce normal levels of viperin grew the virus to higher titres. These anti-dengue effects of viperin were mediated by amino acid residues in its C-terminus, and did not require structural domains of the N-terminal region as has been previously shown by us and others for the related virus, hepatitis C virus. Viperin was also demonstrated to co-localise and interact with the dengue capsid protein on the surface of lipid droplets, as well as with the NS3 protein and viral RNA. Viperin's association with NS3 was further demonstrated to be involved in its anti-dengue activities. The anti-viral activities of viperin presented in this manuscript show both similarities and contrasts with other described anti-viral mechanisms for the protein and highlight the diverse nature of this unique anti-viral host protein.
- Published
- 2013
77. Arabidopsis uses two gluconeogenic gateways for organic acids to fuel seedling establishment
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Eastmond, Peter J., Astley, Holly M., Parsley, Kate, Aubry, Sylvain, Williams, Ben P., Menard, Guillaume N., Craddock, Christian P., Nunes-Nesi, Adriano, Fernie, Alisdair R., Hibberd, Julian M., Hibberd, Julian [0000-0003-0662-7958], and Apollo - University of Cambridge Repository
- Subjects
Two gluconeogenic ,Arabidopsis ,Carbohydrates ,Gluconeogenesis ,Lipid Metabolism ,Article ,Carbon ,Phosphoenolpyruvate Carboxylase ,Pyruvate, Orthophosphate Dikinase ,Gene Expression Regulation, Plant ,Seedlings ,Mutation ,Pyruvic Acid ,Seeds ,Fuel seedling ,Dicarboxylic Acids ,Signal Transduction - Abstract
Gluconeogenesis is a fundamental metabolic process that allows organisms to make sugars from non-carbohydrate stores such as lipids and protein. In eukaryotes only one gluconeogenic route has been described from organic acid intermediates and this relies on the enzyme phosphoenolpyruvate carboxykinase (PCK). Here we show that two routes exist in Arabidopsis, and that the second uses pyruvate, orthophosphate dikinase (PPDK). Gluconeogenesis is critical to fuel the transition from seed to seedling. Arabidopsis pck1 and ppdk mutants are compromised in seed-storage reserve mobilization and seedling establishment. Radiolabelling studies show that PCK predominantly allows sugars to be made from dicarboxylic acids, which are products of lipid breakdown. However, PPDK also allows sugars to be made from pyruvate, which is a major product of protein breakdown. We propose that both routes have been evolutionarily conserved in plants because, while PCK expends less energy, PPDK is twice as efficient at recovering carbon from pyruvate., During seed germination plants use gluconeogenesis to mobilize noncarbohydrate energy reserves. Here Eastmond et al. show that plants, unlike other eukaryotes, do not solely rely on a gluconeogenic pathway via the enzyme PCK but also use a second pathway relying on PPDK.
- Published
- 2015
78. Paradoxical articular manifestations in inflammatory bowel diseases patients treated by anti-TNF
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Thiebault, H., Boyard-Lasselin, P., Guignant, C., Guillaume, N., Wacrenier, A., Brazier, Franck, Nguyen-Khac, Eric, Goeb, V., Dupas, Jean-Louis, Fumery, Mathurin, and DESSAIVRE, Louise
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[SDV] Life Sciences [q-bio] - Published
- 2015
79. Robotic assistance for percutaneous needle insertion in the kidney: preclinical proof on a swine animal model
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Thierry de Baere, Charles Roux, Guillaume Noel, Alexandre Delpla, Frederic Deschamps, Eloi Varin, and Lambros Tselikas
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Ablation techniques ,Biopsy (needle) ,Kidney ,Robot-enhanced procedures ,Tomography (x-ray computed) ,Medical physics. Medical radiology. Nuclear medicine ,R895-920 - Abstract
Abstract Background We evaluated the accuracy, safety, and feasibility of a computed tomography (CT)-guided robotic assistance system for percutaneous needle placement in the kidney. Methods Fiducials surgically implanted into the kidneys of two pigs were used as targets for subsequent robotically-assisted needle insertion. Robotically-assisted needle insertions and CT acquisitions were coordinated using respiratory monitoring. An initial scan volume data set was used for needle insertion planning defining skin entry and target point. Then, needle insertion was performed according to robot positioning. The accuracy of needle placement was evaluated upon the distance between the needle tip and the predefined target on a post needle insertion scan. A delayed contrast-enhanced CT scan was acquired to assess safety. Results Eight needle trajectories were performed with a median procedural time measured from turning on the robotic system to post needle insertion CT scan of 21 min (interquartile range 15.5−26.5 min). Blind review of needle placement accuracy was 2.3 ± 1.2 mm (mean ± standard deviation) in lateral deviation, 0.7 ± 1.7 mm in depth deviation, and 2.8 ± 1.3 mm in three-dimensional Euclidian deviation. All needles were inserted on the first attempt, which determined 100% feasibility, without needle readjustment. The angulation and length of the trajectory did not impact on the needle placement accuracy. Two minor procedure-related complications were encountered: 2 subcapsular haematomas (13 × 6 mm and 35 × 6 mm) in the same animal. Conclusions Robotically-assisted needle insertion was shown feasible, safe and accurate in a swine kidney model. Further larger studies are needed.
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- 2022
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80. Poliovirus immunity among adults in the Democratic Republic of the Congo: a cross-sectional serosurvey
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Vivian H. Alfonso, Arie Voorman, Nicole A. Hoff, William C. Weldon, Sue Gerber, Adva Gadoth, Megan Halbrook, Amelia Goldsmith, Patrick Mukadi, Reena H. Doshi, Guillaume Ngoie-Mwamba, Trevon L. Fuller, Emile Okitolonda-Wemakoy, Jean-Jacques Muyembe-Tamfum, and Anne W. Rimoin
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Poliovirus ,Polio ,Immunization ,Vaccine-preventable diseases ,Adult immunity ,Democratic Republic of the Congo ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Vaccination efforts to eradicate polio currently focus on children under 5 years of age, among whom most cases of poliomyelitis still occur. However, in the Democratic Republic of the Congo (DRC), an outbreak of wild poliovirus type 1 occurred in 2010–2011 in which 16% of cases occurred among adults; in a related outbreak in the neighboring Republic of Congo, 75% of cases occurred among the same adult age-group. Given that infected adults may transmit poliovirus, this study was designed to assess adult immunity against polioviruses. Methods We assessed poliovirus seroprevalence using dried blood spots from 5,526 adults aged 15–59 years from the 2013–2014 Demographic and Health Survey in the DRC. Results Among adults in the DRC, 74%, 72%, and 57% were seropositive for neutralizing antibodies for poliovirus types 1, 2, and 3, respectively. For all three serotypes, seroprevalence tended to be higher among older age groups, those living in households with more children, and among women. Conclusions Protection against poliovirus is generally low among adults in the DRC, particularly for type 3 poliovirus. The lack of acquired immunity in adults suggests a potentially limited poliovirus circulation over the lifetime of those surveyed (spanning 1954 through 2014) and transmission of vaccine-derived poliovirus in this age group while underscoring the risk of these outbreaks among adults in the DRC.
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- 2022
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81. Macroscopic Skin Examination Can Determine the Number of Strips Necessary to Study the stratum corneum in Dogs
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Marion Mosca, Mélanie Legain, Guillaume Noël, Adrien Idée, and Didier Pin
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skin barrier ,non-invasive measurement method ,stratum corneum ,atopic dermatitis ,Veterinary medicine ,SF600-1100 - Abstract
To evaluate the skin barrier, the stratum corneum (SC) must be isolated and extracted. Currently, skin biopsy is the gold standard method to investigate skin immunology and the presence of biomarkers in dogs. However, a standardized, non-invasive tool to exclusively remove the SC would be of great interest to study healthy and atopic dogs. In this study, we performed D-squames® tape stripping with standardized pressure on seven healthy beagle dogs. A control site was defined and then 25 strips, 50 strips and as many strips as needed to achieve a shiny appearance of the skin were performed on three different experimental sites. After stripping, blinded histopathological examination of a skin biopsy from each site was performed. The number of tape strips required for the skin to become shiny varied between individuals, with a mean of 40 (29–50) strips. There was no significant difference in SC depth between the control site and the site that underwent 25 tape strips. In contrast, the use of 50 strips removed almost all of the SC, with a mean remaining SC depth of 7.82 µm. These data suggest that this non-invasive method can effectively remove the SC, with individual variability, and that a shiny appearance of the skin after stripping can be used as an accurate marker of SC removal.
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- 2023
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82. Cascading One-Pot Synthesis of Biodegradable Uronic Acid-Based Surfactants from Oligoalginates, Semi-Refined Alginates, and Crude Brown Seaweeds
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Freddy Pessel, Guillaume Noirbent, Cédric Boyère, Sacha Pérocheau Arnaud, Tiphaine Wong, Laura Durand, and Thierry Benvegnu
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oligomannuronate ,oligoguluronate ,oligoalginate ,semi-refined alginate ,brown seaweed ,one pot cascade process ,Organic chemistry ,QD241-441 - Abstract
The present article describes a one-pot and cascade mode process using biocompatible/biodegradable reagents, for simply obtaining surfactant compositions comprising mixtures of d-mannuronic acid and l-guluronic acid directly from oligoalginates or semi-refined alginates (mixtures of alginate, cellulose, hemicellulose, laminaran, and fucan). Simple treatments of partial purification of the reaction crudes (elimination of the salts and/or the residual fatty alcohols) or isolation of the surfactant compositions result in sugar-based compounds having performance levels appropriate to applications in detergency. In addition, the challenging extension of this cascading one-pot synthesis technology to crude milled brown seaweeds was successfully carried out to provide promising surface-active compositions made up of alkyl uronate and alkyl glycoside monosaccharides.
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- 2023
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83. Corrigendum to 'Examination of scenarios introducing rubella vaccine in the Democratic Republic of the Congo' [Vaccine: X 9 (2021) 100127]
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Alvan Cheng, Kurt Frey, Guillaume Ngoie Mwamba, Kevin A. McCarthy, Nicole A. Hoff, and Anne W. Rimoin
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Immunologic diseases. Allergy ,RC581-607 - Published
- 2022
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84. Usages et perceptions des outils de traduction automatique : une enquête auprès d'apprenants Lansad
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Guillaume Nassau, Nicolas Molle, and Carmenne Kalyaniwala
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autonomy ,LANSOD ,representations ,machine translation ,Special aspects of education ,LC8-6691 ,Philology. Linguistics ,P1-1091 - Abstract
The use of machine translation (MT) tools is already widely spread among students as part of their language training. Our survey, carried out with over 400 Lansod students, aims to identify the tools used by students, to describe their procedures and to gather information regarding the perception of these tools. Results reveal that the use of these tools is often out of step with their conception, on the one hand because of a general confusion concerning MT tools, and on the other hand, because of insufficient awareness of their role in language learning. This first step in the Lansod sector underlines the need to train students in MT tools. It prompts us to further question the role of teachers in the handling of these tools by students.
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- 2022
85. Establishing Normative Dynamic Postural Control Values in Elite Female Handball Players
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Brice Picot, Jeanne Dury, Guillaume Néron, Patrick O McKeon, and Nicolas Forestier
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Sports medicine ,RC1200-1245 - Abstract
# Background Lower extremity injuries among young female handball players are very common. The modified Star Excursion Balance Test (mSEBT) is a valid clinical tool to assess dynamic postural control and identify athletes with higher risk of injury. However, its interpretation is difficult since performance on this test is highly sport dependent. No normative values on the mSEBT exist in handball. # Purpose The aim of this investigation was to establish normative ranges of mSEBT performance in young, healthy female handball players to help practitioners when interpreting risk estimates. # Study design Cross-Sectional Study # Methods Athletes from 14 elite teams were recruited during a national tournament and performed 3 trials in the anterior (ANT), posteromedial (PM), posterolateral (PL) directions of the mSEBT. Means, standard deviations and 95% confidence intervals (95%CI) of normalized reached distances were calculated for each direction and the composite score (COMP). Level of asymmetry between dominant and non-dominant limbs were calculated for each direction using Bland Altman analyses. Group differences were weighed against the established mSEBT minimum detectable differences (MDD) to compare scores between limbs and across different player positions. # Results One-hundred and eighty-eight females (16.8±0.9 years) were tested. Mean reach distances were 65.2±5% (64.7-65.7), 110.0±6.2% (109.3-110.6), 107.1±6.2% (106.5-107.8) and 94.1±4.9% (93.6-94.6) for the ANT, PM, PL directions and COMP score respectively. Bias and limits of agreement for limb asymmetry were -0.23% (-5.85%, 5.38%) for ANT, -0.83% (-8.80%, 7.14%) for PM, 0.33% (-8.51%, 9.17%) for PL and -0.27% (-4.88%, 4.33%) for COMP score. No meaningful differences were observed between limbs or across player positions since the values did not exceed the MDD and all 95%CIs overlapped. # Conclusion This study provides normative performance values for dynamic postural control as measured by the mSEBT among young, healthy, elite female handball players. Considering the high incidence of injury in this population, these values can be used for injury risk reduction and return to sport decisions. Further prospective studies are needed to established specific cut-off scores in this population. # Level of evidence 2c
- Published
- 2022
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86. HCV RNA traffic and association with NS5A in living cells
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Fiches, Guillaume N., primary, Eyre, Nicholas S., additional, Aloia, Amanda L., additional, Van Der Hoek, Kylie, additional, Betz-Stablein, Brigit, additional, Luciani, Fabio, additional, Chopra, Abha, additional, and Beard, Michael R., additional
- Published
- 2016
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87. Natural killer cell licensing after double cord blood transplantation is driven by the self-HLA class I molecules from the dominant cord blood
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Guillaume, N., primary, Loiseau, P., additional, Gagne, K., additional, Moins-Teissserenc, H., additional, Cayuela, J.-M., additional, Henry, G., additional, Robin, M., additional, de Latour, R. P., additional, Gluckman, E., additional, Socie, G., additional, Retiere, C., additional, Dulphy, N., additional, and Toubert, A., additional
- Published
- 2016
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88. Towards new NIR dyes for free radical photopolymerization processes
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Haifaa Mokbel, Guillaume Noirbent, Didier Gigmes, Frédéric Dumur, and Jacques Lalevée
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cyanine ,nir light ,photochemistry ,Science ,Organic chemistry ,QD241-441 - Abstract
The use of cheap and safe near-infrared (NIR) light is still the subject of intense research efforts but remains a huge challenge due to the associated low photon energy (wavelength from 0.78 to 2.5 µm). In this study, a series of 17 NIR dyes mainly based on a well-established cyanine scaffold is proposed. Remarkably, 11 of them were never synthesized before. Markedly, noncharged structures, negatively charged cyanine bearing Na+ as counter cation, and positively charged cyanines bearing (B(Ph)4−) or (I−) as counter anions were examined as promising NIR light photoinitiating systems. Excellent photoinitiating abilities were found for some reported dyes when used in combination with iodonium salt and amine. Markedly, photothermal effects with a huge heater behavior were also observed for different NIR dye structures. Interestingly, the synthesis of interpenetrating polymer networks (IPNs, e.g., for the polymerization of acrylate/epoxy monomer blends) can also be carried out upon NIR light with the proposed systems.
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- 2021
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89. Study and characterization of the irreversible transformation of electrically stressed planar Ti/TiOx/Ti junctions
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Guillaume, N., primary, Puyoo, E., additional, Le Berre, M., additional, Albertini, D., additional, Baboux, N., additional, Chevalier, C., additional, Ayadi, K., additional, Grégoire, J., additional, Gautier, B., additional, and Calmon, F., additional
- Published
- 2015
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90. Bone complications of mastocytosis: a link between clinical and biological characteristics
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Guillaume, N., Desoutter, J., Chandresis, O., Merlusca, L., Henry, I., Georgin-Lavialle, S., Barete, S., Hirsh, I., Bouredji, D., Royer, B., Gruson, B., Lok, C., Seveste, H., Mentaverri, R., Brazier, M., Meynier, J., Hermine, O., J.P., Marolleau, Kamel, Saïd, Pharm, D., Damaj, G., Cytokines, hématopoïèse et réponse immune (CHRI), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Centre National de la Recherche Scientifique (CNRS), and Slama, Catherine
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ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2013
91. A Tumour and Liver Automatic Segmentation (ATLAS) Dataset on Contrast-Enhanced Magnetic Resonance Imaging for Hepatocellular Carcinoma
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Félix Quinton, Romain Popoff, Benoît Presles, Sarah Leclerc, Fabrice Meriaudeau, Guillaume Nodari, Olivier Lopez, Julie Pellegrinelli, Olivier Chevallier, Dominique Ginhac, Jean-Marc Vrigneaud, and Jean-Louis Alberini
- Subjects
MRI ,liver ,liver tumours ,TARE ,hepatocellular carcinoma ,Bibliography. Library science. Information resources - Abstract
Liver cancer is the sixth most common cancer in the world and the fourth leading cause of cancer mortality. In unresectable liver cancers, especially hepatocellular carcinoma (HCC), transarterial radioembolisation (TARE) can be considered for treatment. TARE treatment involves a contrast-enhanced magnetic resonance imaging (CE-MRI) exam performed beforehand to delineate the liver and tumour(s) in order to perform dosimetry calculation. Due to the significant amount of time and expertise required to perform the delineation process, there is a strong need for automation. Unfortunately, the lack of publicly available CE-MRI datasets with liver tumour annotations has hindered the development of fully automatic solutions for liver and tumour segmentation. The “Tumour and Liver Automatic Segmentation” (ATLAS) dataset that we present consists of 90 liver-focused CE-MRI covering the entire liver of 90 patients with unresectable HCC, along with 90 liver and liver tumour segmentation masks. To the best of our knowledge, the ATLAS dataset is the first public dataset providing CE-MRI of HCC with annotations. The public availability of this dataset should greatly facilitate the development of automated tools designed to optimise the delineation process, which is essential for treatment planning in liver cancer patients.
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- 2023
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92. Single cell RNA sequencing identifies IGFBP5 and QKI as ciliated epithelial cell genes associated with severe COPD
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Xiuying Li, Guillaume Noell, Tracy Tabib, Alyssa D. Gregory, Humberto E. Trejo Bittar, Ravi Vats, Tomasz W. Kaminski, John Sembrat, Mark E. Snyder, Divay Chandra, Kong Chen, Chunbin Zou, Yingze Zhang, Prithu Sundd, John F. McDyer, Frank Sciurba, Mauricio Rojas, Robert Lafyatis, Steve D. Shapiro, Rosa Faner, and Toru Nyunoya
- Subjects
Single cell RNA-seq ,COPD ,Cigarette smoke ,Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background Whole lung tissue transcriptomic profiling studies in chronic obstructive pulmonary disease (COPD) have led to the identification of several genes associated with the severity of airflow limitation and/or the presence of emphysema, however, the cell types driving these gene expression signatures remain unidentified. Methods To determine cell specific transcriptomic changes in severe COPD, we conducted single-cell RNA sequencing (scRNA seq) on n = 29,961 cells from the peripheral lung parenchymal tissue of nonsmoking subjects without underlying lung disease (n = 3) and patients with severe COPD (n = 3). The cell type composition and cell specific gene expression signature was assessed. Gene set enrichment analysis (GSEA) was used to identify the specific cell types contributing to the previously reported transcriptomic signatures. Results T-distributed stochastic neighbor embedding and clustering of scRNA seq data revealed a total of 17 distinct populations. Among them, the populations with more differentially expressed genes in cases vs. controls (log fold change >|0.4| and FDR = 0.05) were: monocytes (n = 1499); macrophages (n = 868) and ciliated epithelial cells (n = 590), respectively. Using GSEA, we found that only ciliated and cytotoxic T cells manifested a trend towards enrichment of the previously reported 127 regional emphysema gene signatures (normalized enrichment score [NES] = 1.28 and = 1.33, FDR = 0.085 and = 0.092 respectively). Among the significantly altered genes present in ciliated epithelial cells of the COPD lungs, QKI and IGFBP5 protein levels were also found to be altered in the COPD lungs. Conclusions scRNA seq is useful for identifying transcriptional changes and possibly individual protein levels that may contribute to the development of emphysema in a cell-type specific manner.
- Published
- 2021
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93. Le CRAPEL, l’autonomie et l’autonomisation : la continuité des travaux à l’Université de Lorraine
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Guillaume NASSAU
- Subjects
Autonomisation ,advising ,affective aspects ,emotional labour ,self-directed language learning scheme ,Autonomy ,Special aspects of education ,LC8-6691 ,Theory and practice of education ,LB5-3640 - Abstract
This paper discusses the recent history of research and pedagogical practice in the field of autonomous language learning. First, the work of the Crapel is examined through one of the topics that has recently been the focus of a team effort: the affective dimension of both self-directed language learning and language learning advising. An overview of the self-directed learning schemes currently in use at the Université de Lorraine is then provided, showing the diversity of ways in which local language training has taken up the Crapel approach.
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- 2022
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94. Dimension psychologique de l’apprentissage en autonomie
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Guillaume NASSAU
- Subjects
Autonomy ,Autonomisation ,autonomous process ,Special aspects of education ,LC8-6691 ,Theory and practice of education ,LB5-3640 - Published
- 2022
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95. Arabidopsis uses two gluconeogenic gateways for organic acids to fuel seedling establishment
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Eastmond, Peter J., primary, Astley, Holly M., additional, Parsley, Kate, additional, Aubry, Sylvain, additional, Williams, Ben P., additional, Menard, Guillaume N., additional, Craddock, Christian P., additional, Nunes-Nesi, Adriano, additional, Fernie, Alisdair R., additional, and Hibberd, Julian M., additional
- Published
- 2015
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96. Impact of contouring methods on pre-treatment and post-treatment dosimetry for the prediction of tumor control and survival in HCC patients treated with selective internal radiation therapy
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Guillaume Nodari, Romain Popoff, Jean Marc Riedinger, Olivier Lopez, Julie Pellegrinelli, Inna Dygai-Cochet, Claire Tabouret-Viaud, Benoit Presles, Olivier Chevallier, Sophie Gehin, Matthieu Gallet, Marianne Latournerie, Sylvain Manfredi, Romaric Loffroy, Jean Marc Vrigneaud, and Alexandre Cochet
- Subjects
Radioembolization ,Hepatocellular carcinoma ,Dosimetry ,Medical physics. Medical radiology. Nuclear medicine ,R895-920 - Abstract
Abstract Introduction The aim of this study was to evaluate the impact of the contouring methods on dose metrics and their predictive value on tumor control and survival, in both situations of pre-treatment and post-treatment dosimetry, for patients with advanced HCC treated with SIRT. Methods Forty-eight patients who underwent SIRT between 2012 and 2020 were retrospectively included in this study. Target volumes were delineated using two methods: MRI-based contours manually drawn by a radiologist and then registered on SPECT/CT and PET/CT via deformable registration (Pre-CMRI and Post-CMRI), 99mTc-MAA-SPECT and 90Y-microspheres-PET 10% threshold contouring (Pre-CSPECT and Post-CPET). The mean absorbed dose (Dm) and the minimal absorbed dose delivered to 70% of the tumor volume (D70) were evaluated with both contouring methods; the tumor-to-normal liver uptake ratio (TNR) was evaluated with MRI-based contours only. Tumor response was assessed using the mRECIST criteria on the follow-up MRIs. Results No significant differences were found for Dm and TNR between pre- and post-treatment. TNR evaluated with radiologic contours (Pre-CMRI and Post-CMRI) were predictive of tumor control at 6 months on pre- and post-treatment dosimetry (OR 5.9 and 7.1, respectively; p = 0.02 and 0.01). All dose metrics determined with both methods were predictive of overall survival (OS) on pre-treatment dosimetry, but only Dm with MRI-based contours was predictive of OS on post-treatment images with a median of 23 months for patients with a supramedian Dm versus 14 months for the others (p = 0.04). Conclusion In advanced HCC treated with SIRT, Dm and TNR determined with radiologic contours were predictive of tumor control and OS. This study shows that a rigorous clinical workflow (radiologic contours + registration on scintigraphic images) is feasible and should be prospectively considered for improving therapeutic strategy.
- Published
- 2021
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97. Feasibility, safety and accuracy of a CT-guided robotic assistance for percutaneous needle placement in a swine liver model
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Boris Guiu, Thierry De Baère, Guillaume Noel, and Maxime Ronot
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Medicine ,Science - Abstract
Abstract Evaluate the feasibility, safety and accuracy of a CT-guided robotic assistance for percutaneous needle placement in the liver. Sixty-six fiducials were surgically inserted into the liver of ten swine and used as targets for needle insertions. All CT-scan acquisitions and robotically-assisted needle insertions were coordinated with breath motion using respiratory monitoring. Skin entry and target points were defined on planning CT-scan. Then, robotically-assisted insertions of 17G needles were performed either by experienced interventional radiologists or by a novice. Post-needle insertion CT-scans were acquired to assess accuracy (3D deviation, ie. distance from needle tip to predefined target) and safety. All needle insertions (43/43; median trajectory length = 83 mm (interquartile range [IQR] 72–105 mm) could be performed in one (n = 36) or two (n = 7) attempts (100% feasibility). Blinded evaluation showed an accuracy of 3.5 ± 1.3 mm. Accuracy did not differ between novice and experienced operators (3.7 ± 1.3 versus 3.4 ± 1.2 mm, P = 0.44). Neither trajectory angulation nor trajectory length significantly impacted accuracy. No complications were encountered. Needle insertion using the robotic device was shown feasible, safe and accurate in a swine liver model. Accuracy was influenced neither by the trajectory length nor by trajectory angulations nor by operator’s experience. A prospective human clinical trial is recruiting.
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- 2021
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98. The Use of Sacrificial Graphite-like Coating to Improve Fusion Efficiency of Copper in Selective Laser Melting
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Angela Elisa Crespi, Guillaume Nordet, Patrice Peyre, Charles Ballage, Marie-Christine Hugon, Patrick Chapon, and Tiberiu Minea
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selective laser melting ,copper ,amorphous carbon ,sacrificial layer ,interferences ,power density ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
Thin and ultrathin carbon films reduce the laser energy required for copper powder fusion in selective laser melting (SLM). The low absorption of infrared (IR) radiation and its excellent thermal conductivity leads to an intricate combination of processing parameters to obtain high-quality printed parts in SLM. Two carbon-based sacrificial thin films were deposited onto copper to facilitate light absorption into the copper substrates. Graphite-like (3.5 µm) and ultra-thin (25 nm) amorphous carbon films were deposited by aerosol spraying and direct current magnetron sputtering, respectively. The melting was analyzed for several IR (1.06 µm) laser powers in order to observe the coating influence on the energy absorption. Scanning electron microscopy showed the topography and cross-section of the thermally affected area, electron backscatter diffraction provided the surface chemical composition of the films, and glow-discharge optical emission spectroscopy (GDOES) allowed the tracking of the in-deep chemical composition of the 3D printed parts using carbon film-covered copper. Ultra-thin films of a few tens of nanometers could reduce fusion energy by about 40%, enhanced by interferences phenomena. Despite the lower energy required, the melting maintained good quality and high wettability when using top carbon coatings. A copper part was SLM printed and associated with 25 nm of carbon deposition between two copper layers. The chemical composition analysis demonstrated that the carbon was intrinsically removed during the fusion process, preserving the high purity of the copper part.
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- 2023
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99. A device to simulate contaminant transfer and surface and subsurface flow through intact soil monoliths
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Nico Hachgenei, Guillaume Nord, Lorenzo Spadini, Henri Mora, François Courvoisier, Jean‐François Sutra, Jean‐Pierre Vandervaere, Cédric Legoût, Marie‐Christine Morel, Jean Martins, Anne Lespine, and Celine Duwig
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Environmental sciences ,GE1-350 ,Geology ,QE1-996.5 - Abstract
Abstract Many contaminants of agricultural origin are released into rural environments, particularly at the soil surface. Their fate has been extensively investigated in repacked soils, but only few studies have addressed their transport in structurally preserved natural soils. Much remains unknown about their fate and transfer within and between environmental compartments, while the susceptibility of these compartments to the contaminants adverse effects can vary considerably. The lack of studies regarding surface and subsurface transfer of contaminants through intact soil compared with studies on repacked soil led us to propose a device and protocol for sampling intact soil monoliths (60 × 30 × 22 cm3, length, width, depth [LWD]) without heavy machinery. This is achieved by a modular design with removable top and bottom lid and a protocol of cutting the soil and replacing the affected bottom soil with a drainage layer of glass beads. The device allows the application of artificial rainfall events with simultaneous highly resolved quantification of infiltration excess overland flow and drainage discharge. It is designed to facilitate the collection of samples for physical, biological, and chemical analyses that fulfill cleanliness standards for organic contaminant analysis at trace levels using only poorly reactive stainless steel and glass materials. Testing of the device was performed by measuring the transfer of the antiparasitic drug ivermectin (IVM) through and over a silt‐loam pasture soil. This test case illustrates how the device can be used to gain valuable information on the transfer of trace organic contaminants through topsoils.
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- 2022
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100. Faster and more precise isotopic water analysis of discrete samples by predicting the repetitions’ asymptote instead of averaging last values
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Nico Hachgenei, Véronique Vaury, Guillaume Nord, Lorenzo Spadini, and Céline Duwig
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Asymptotic Approximation Calibration in Cavity Ring-Down Spectroscopy (CRDS) ,Science - Abstract
Water stable isotope analysis using Cavity Ring-Down Spectroscopy (CRDS) has a strong between-sample memory effect. The classic approach to correct this memory effect is to inject the sample at least 6 times and ignore the first two to three injections. The average of the remaining injections is then used as measured value. This is in many cases insufficient to completely compensate the memory effect. We propose a simple approach to correct this memory effect by predicting the asymptote of consecutive repeated injections instead of averaging over them. The asymptote is predicted by fitting a y=ax+b relation to the sample repetitions and keeping b as measured value. This allows to save analysis time by doing less injections while gaining precision. We provide a Python program applying this method and describe the steps necessary to implement this method in any other programming language. We also show validation data comparing this method to the classical method of averaging over the last couple of injections. The validation suggests a gain in time of a factor two while gaining in precision at the same time. The method does not have any specific requirements for the order of analysis and can therefore also be applied to an existing set of analyzes in retrospect. • We fit a simple y=ax+b relation to the sample repetitions of Picarro L2130-i isotopic water analyzer, in order to keep the asymptote (b) as measured value instead of using the average over the last couple of measurements. • This allows a higher precision in the measured value with less repetitions of the injection saving precious time during analysis. • We provide a sample code using Python, but generally this method is easy to implement in any automated data treatment protocol.
- Published
- 2022
- Full Text
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