423 results on '"Gui Hu"'
Search Results
52. Study on Enhancing the Performance of Unglazed Solar Air Collector by Attached Jet
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Jian Jin, Jianjun Hu, Gui Hu, and Shenghua Du
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- 2022
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53. Incidence of bifid pancreatic duct in pancreaticoduodenectomy and its impact on clinically relevant postoperative pancreatic fistula
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Liu, Ouyang, Hao, Hu, Gang, Nie, Li-Xue, Yang, Zhi-Ping, Huang, Chen-Ming, Ni, Zhuo, Shao, Kai-Lian, Zheng, Wei, Jing, Bin, Song, Gang, Li, Xian-Gui, Hu, and Gang, Jin
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Cancer Research ,Oncology - Abstract
ObjectivesThis study aimed to examine the incidence of bifid pancreatic duct (BPD) in pancreaticoduodenectomy (PD) and clarify its impact on clinically relevant postoperative pancreatic fistula (CR-POPF).BackgroundUntil now, all the literature about BPD during PD are published as case reports, and the incidence of BPD in PD and its impact on CR-POPF remain unknown.ResultsA total of 438 consecutive PDs were divided into two groups: the former year group and the latter year group. The former year group included 215 consecutive PDs, while the latter year group included 223. In the latter year group, we found 16 BPDs during PD (O-BPD); the incidence of O-BPD is 7.17%. Of them, there were eight patients who had BPD in the preoperative imaging (I-BPD). All the I-BPDs are O-BPDs; which means that 50% of O-BPDs were a single pancreatic duct in the preoperative imaging (I-SPD). There were 17 I-BPDs in the 438 consecutive PDs; the incidence of I-BPD is 3.88%. In the former year group, the rate of severe complications of I-BPD and I-SPD is 77.78% and 27.18%, respectively (p = 0.003); the rate of CR-POPF of I-BPD is higher than I-SPD, 55.56% vs. 27.18%, but there were no statistically significant differences. In the latter year group, the rate of severe complications of O-BPD and O-SPD is 50% and 18.36%, and the rate of CR-POPF of O-BPD and O-SPD is 37.5% and 22.22%, respectively; both of them have statistically significant differences, and the p-value is 0.003 and 0.006, respectively. In the subgroup analysis, both the rate of severe complications and the rate of CR-POPF of I-BPD were higher than O-BPD, 77.78% vs. 50%, and 55.56% vs. 37.5%, but there were no statistically significant differences in both of them; the p-value is 0.174 and 0.434, respectively. Univariate and multivariate analyses showed that BPD was an independent risk factor of CR-POPF.ConclusionsThe incidence of O-BPD in PD is 7.17%, 50% of O-BPDs were I-SPD, and the incidence of I-BPD is 3.88%. BPD is an independent risk factor of CR-POPF. The suture closure method may be a simple, safe, and effective method in dealing with BPD in PD. more...
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- 2022
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54. Assessment of MODIS, MERIS, GEOV1 FPAR Products over Northern China with Ground Measured Data and by Analyzing Residential Effect in Mixed Pixel.
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Fei Yang, Hongyan Ren, Xiaoyu Li, Mao-Gui Hu, and Yaping Yang
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- 2014
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55. Circular RNAs of UDP-Glycosyltransferase (UGT) Genes Expand the Complexity and Diversity of the UGT Transcriptome
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Ross A. McKinnon, Peter I. Mackenzie, Dong Gui Hu, Robyn Meech, and Julie-Ann Hulin
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0301 basic medicine ,Pharmacology ,Genetics ,UGT2B4 ,Alternative splicing ,Biology ,digestive system ,Transcriptome ,03 medical and health sciences ,Open reading frame ,030104 developmental biology ,0302 clinical medicine ,Start codon ,Complementary DNA ,RNA splicing ,Molecular Medicine ,Gene ,030217 neurology & neurosurgery - Abstract
The human UDP-glycosyltransferase (UGT) gene superfamily generates 22 canonical transcripts coding for functional enzymes and also produces nearly 150 variant UGT transcripts through alternative splicing and intergenic splicing. In the present study, our analysis of circRNA databases identified backsplicing events that predicted 85 circRNAs from UGT genes, with 33, 11, and 19 circRNAs from UGT1A, UGT2B4, UGT8, respectively. Most of these UGT circRNAs were reported by one database and had low abundance in cell- or tissue-specific contexts. Using reverse-transcriptase polymerase chain reaction with divergent primers and cDNA samples from human tissues and cell lines, we found 13 circRNAs from four UGT genes: UGT1A (three), UGT2B7 (one), UGT2B10 (one), and UGT8 (eight). Notably, all eight UGT8 circRNAs contain open reading frames that include the canonical start AUG codon and encode variant proteins that all have the common 274–amino acidN-terminal region of wild-type UGT8 protein. We further showed that one UGT8 circRNA (circ_UGT8-1) was broadly expressed in human tissues and cell lines, resistant to RNase R digestion, and predominately present in the cytoplasm. We cloned five UGT8 circRNAs into the Zinc finger with KRAB and SCAN domains 1 vector and transfected them into HEK293T cells. All these vectors produced both circRNAsand linear transcripts with varying circular/linear ratios (0.17–1.14).Western blotting and mass spectrometry assays revealed that only linear transcripts and not circRNAs were translated. In conclusion, our findings of nearly 100 circRNAs greatly expand the complexity and diversity of the UGT transcriptome; however, UGT circRNAs are expressed at a very low level in specific cellular contexts, and their biologic functions remain to be determined. SIGNIFICANCE STATEMENT The human UGT gene transcriptome comprises 22 canonical transcripts coding for functional enzymes and approximately 150 alternatively spliced and chimeric variant transcripts. The present study identified nearly 100 circRNAs from UGT genes, thus greatly expanding the complexity and diversity of the UGT transcriptome. UGT circRNAs were expressed broadly in human tissues and cell lines; however, most showed very low abundance in tissue- and cell-specific contexts, and therefore their biological functions remain to be investigated. more...
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- 2021
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56. A B-SHADE based best linear unbiased estimation tool for biased samples.
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Mao-Gui Hu, Jinfeng Wang 0001, Yu Zhao, and Lin Jia
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- 2013
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57. Design-based spatial sampling: Theory and implementation.
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Jinfeng Wang 0001, Cheng-Sheng Jiang, Mao-Gui Hu, Zhidong Cao, Yansha Guo, Lianfa Li, Tiejun Liu, and Bin Meng
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- 2013
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58. Source Characteristics and Exacerbated Tsunami Hazard of the 2020 Mw 6.9 Samos Earthquake in Eastern Aegean Sea
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Gui Hu, Wanpeng Feng, Yuchen Wang, Linlin Li, Xiaohui He, Çağıl Karakaş, and Yunfeng Tian
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Geophysics ,Space and Planetary Science ,Geochemistry and Petrology ,Earth and Planetary Sciences (miscellaneous) - Published
- 2022
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59. Comparison of different methods for corn LAI estimation over northeastern China.
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Fei Yang, Jiulin Sun, Hongliang Fang, Zuofang Yao, Jiahua Zhang, Yunqiang Zhu, Kaishan Song, Zongming Wang, and Mao-Gui Hu
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- 2012
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60. Predictors of lymph-node metastasis in surgically resected T1 colorectal cancer in Western populations
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Peng Deng, Fuxin Tang, Hui Li, Ce-Gui Hu, Chenghao Yi, Zhi-Yang Liu, Taicheng Zhou, and Zhen Zong
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Oncology ,medicine.medical_specialty ,Colorectal cancer ,overall survival ,medicine.medical_treatment ,Rectum ,Lower risk ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Epidemiology ,medicine ,Neoadjuvant therapy ,AcademicSubjects/MED00260 ,business.industry ,Hazard ratio ,end results database ,Gastroenterology ,Original Articles ,medicine.disease ,Confidence interval ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,lymph-node metastasis ,surveillance ,T1 colorectal cancer ,epidemiology ,030211 gastroenterology & hepatology ,business - Abstract
Background The risk of lymph-node metastasis (LNM) in T1 colorectal cancer (CRC) has not been well documented in heterogeneous Western populations. This study investigated the predictors of LNM and the long-term outcomes of patients by analysing T1 CRC surgical specimens and patients’ demographic data. Methods Patients with surgically resected T1 CRC between 2004 and 2014 were identified from the Surveillance, Epidemiology, and End Results (SEER) database. Patients with multiple primary cancers, with neoadjuvant therapy, or without a confirmed histopathological diagnosis were excluded. Multivariate logistic-regression analysis was used to identify the predictors of LNM. Results Of the 22,319 patients, 10.6% had a positive lymph-node status based on the final pathology (nodal category: N1 9.6%, N2 1.0%). Younger age, female sex, Asian or African-American ethnicity, poor differentiation, and tumor site outside the rectum were significantly associated with LNM. Subgroup analyses for patients stratified by tumor site suggested that the rate of positive lymph-node status was the lowest in the rectum (hazard ratio: 0.74; 95% confidence interval: 0.63–0.86). Conclusion The risk of LNM was potentially lower in Caucasian patients than in API or African-American patients with surgically resected T1 CRC. Regarding the T1 CRC site, the rectum was associated with a lower risk of LNM. more...
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- 2021
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61. MicroRNA-133b expression inversely correlates with MET and can serve as an optimum predictive biomarker for patients of colorectal cancer
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Yihang Guo, Ni Gong, Biao Xie, and Gui Hu
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Oncology ,Cancer Research ,medicine.medical_specialty ,diagnostic biomarker discovery ,Colorectal cancer ,business.industry ,digestive, oral, and skin physiology ,medicine.disease ,microRNA-133b ,digestive system diseases ,Colorectal cancer (CRC) ,mesenchymal-to-epithelial transition ,Internal medicine ,microRNA ,medicine ,Original Article ,Radiology, Nuclear Medicine and imaging ,business ,Predictive biomarker - Abstract
Background Inactivation of the tumor suppressor gene microRNA-133b is a frequent event in various malignancies including colorectal cancer (CRC). The result of our previous research has found a statistically significant downregulation of microRNA-133b expression in human CRC cells, microRNA-133b can block the growth and metastatic progression of CRC cells in vitro and in vivo by targeting and repressing mesenchymal to epithelial transition factor. In this study, we identify the association between microRNA-133b, epithelial transition factor expression and clinicopathological parameters. Methods The detection of microRNA-133b and epithelial transition factor between gene and protein expression was evaluated in 46 patients with CRC. The correlation among microRNA-133b, epithelial transition factor and clinicopathological parameters associated with CRC was assessed. Furthermore, the diagnostic capability of microRNA-133b and epithelial transition factor in CRC was also evaluated. Results The results show decreased microRNA-133b expression in CRC tissues, and a very strong inverse correlation between the expression of oncogene epithelial transition factor and the expression of microRNA-133b. We also observed that the expression levels of microRNA-133b and epithelial transition factor were statistical associated with clinical stage and lymph node metastasis in CRC tissues, and further identified microRNA-133b and/or epithelial transition factor may be useful for distinguishing CRC patients from the normal population by receiver operating characteristic (ROC) curve analysis. Conclusions microRNA-133b expression is inversely correlated with epithelial transition factor expression. MicroRNA-133b and/or epithelial transition factor may be useful as valuable prognostic biomarkers in CRC patients. more...
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- 2021
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62. Modeling Spatial Means of Surfaces With Stratified Nonhomogeneity.
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Jinfeng Wang 0001, George Christakos, and Mao-Gui Hu
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- 2009
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63. Super-Resolution Reconstruction of Remote Sensing Images Using Multifractal Analysis.
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Mao-Gui Hu, Jinfeng Wang 0001, and Yong Ge
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- 2009
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64. Identification of key genes controlling cancer stem cell characteristics in gastric cancer
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Jun Huang, Zhi-Kun Ning, Zhengming Zhu, Ce-Gui Hu, and Chao Huang
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medicine.medical_specialty ,Sister chromatid segregation ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,Cancer stem cell ,medicine ,The Cancer Genome Atlas database ,Gene ,Weighted gene coexpression network analysis ,biology ,business.industry ,CENPF ,Basic Study ,Cell cycle ,medicine.disease ,Surgery ,mRNA expression-based stemness index ,Key gene ,030220 oncology & carcinogenesis ,Cancer research ,biology.protein ,Adenocarcinoma ,030211 gastroenterology & hepatology ,Stem cell ,Gastric cancer ,business - Abstract
BACKGROUND Self-renewal of gastric cancer stem cells (GCSCs) is considered to be the underlying cause of the metastasis, drug resistance, and recurrence of gastric cancer (GC). AIM To characterize the expression of stem cell-related genes in GC. METHODS RNA sequencing results and clinical data for gastric adenoma and adenocarcinoma samples were obtained from The Cancer Genome Atlas database, and the results of the GC mRNA expression-based stemness index (mRNAsi) were analyzed. Weighted gene coexpression network analysis was then used to find modules of interest and their key genes. Survival analysis of key genes was performed using the online tool Kaplan-Meier Plotter, and the online database Oncomine was used to assess the expression of key genes in GC. RESULTS mRNAsi was significantly upregulated in GC tissues compared to normal gastric tissues (P < 0.0001). A total of 16 modules were obtained from the gene coexpression network; the brown module was most positively correlated with mRNAsi. Sixteen key genes (BUB1, BUB1B, NCAPH, KIF14, RACGAP1, RAD54L, TPX2, KIF15, KIF18B, CENPF, TTK, KIF4A, SGOL2, PLK4, XRCC2, and C1orf112) were identified in the brown module. The functional and pathway enrichment analyses showed that the key genes were significantly enriched in the spindle cellular component, the sister chromatid segregation biological process, the motor activity molecular function, and the cell cycle and homologous recombination pathways. Survival analysis and Oncomine analysis revealed that the prognosis of patients with GC and the expression of three genes (RAD54L, TPX2, and XRCC2) were consistently related. CONCLUSION Sixteen key genes are primarily associated with stem cell self-renewal and cell proliferation characteristics. RAD54L, TPX2, and XRCC2 are the most likely therapeutic targets for inhibiting the stemness characteristics of GC cells. more...
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- 2020
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65. Expression Profile of GINS Complex Predicts the Prognosis of Pancreatic Cancer Patients
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Xuan Yi, Chen Luo, Chao Huang, Ce-Gui Hu, Zitao Liu, Jiefeng Zhao, Jun Huang, Kang Lin, Xiaojian Zhu, Wen-jun Zhang, Fanqin Bu, and Jinfeng Zhu
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0301 basic medicine ,Cell ,Biology ,medicine.disease ,GINS ,Blot ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Bioinformatics databases ,Oncology ,Pancreatic cancer cell ,030220 oncology & carcinogenesis ,Pancreatic cancer ,medicine ,Cancer research ,Pharmacology (medical) ,Prognostic biomarker ,Gene - Abstract
Background The GINS complex has been implicated in the prognosis of various cancers. It comprises four subunits, encoded by GINS1, GINS2, GINS3, and GINS4 genes. Based on the current understanding, no report exists on the role of the GINS complex in pancreatic cancer. Methods We employed various bioinformatics databases including GEPIA, UALCAN, GEPIA2, and Kaplan Meier Plotter to identify the expression profile of the four genes (GINS1, GINS2, GINS3, and GINS4), their correlation with pancreatic cancer grade as well as their prognostic value of in pancreatic cancer. Western blotting and qRT-PCR analyses were conducted to verify the expression profiles of the four genes in pancreatic cancer. CCK8 and EdU cell experiments were conducted to reveal the role played by the four genes in pancreatic cancer cell proliferation. Results Based on GEPIA, Western blotting, and qRT-PCR analyses, all the four genes in the GINS complex were overexpressed in pancreatic cancer. Notably, the expression of each member was significantly associated with pancreatic cancer grade. The prognostic analysis revealed that not only the whole GINS complex but also each individual were prognostic biomarkers for pancreatic cancer. CCK8 and EdU experiments demonstrated that inhibition of the expression of each GINS member lowered pancreatic cancer cell proliferation. Conclusion This work implicated GINS1, GINS2, GINS3, and GINS4 genes as critical prognostic markers for pancreatic cancer. more...
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- 2020
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66. LINC00657 promotes colorectal cancer stem-like cell invasion by functioning as a miR-203a sponge
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Gui Hu, Chao Liu, Haibo Yu, Lian Zhao, Kaimin Xiang, Hong Xiang, and Shipeng Yan
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0301 basic medicine ,Colorectal cancer ,Biophysics ,Biology ,Biochemistry ,Metastasis ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,law ,Cell Line, Tumor ,medicine ,Humans ,Neoplasm Invasiveness ,Molecular Biology ,Stem like cell ,Oncogene ,Competing endogenous RNA ,Cancer ,Cell Biology ,medicine.disease ,digestive system diseases ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,030220 oncology & carcinogenesis ,Neoplastic Stem Cells ,Cancer research ,Suppressor ,RNA, Long Noncoding ,Colorectal Neoplasms ,Function (biology) - Abstract
Recently, the role of long non-coding RNAs (lncRNAs) in regulating multiple cancer types has attracted increasing interest because of their involvement in cell metastasis in different cancer types. Previous studies indicated that LINC00657 may work as an oncogene in gastric and colon cancer. However, the functional role and mechanistic action of LINC00657 on colorectal cancer (CRC) remains unknown. Therefore, in this study, the role of LINC00657 in CRC was evaluated. Our results showed that LINC00657 was enriched in CRC stem-like cells (CSCs) and significantly promoted CSCs invasion ability. LINC00657 expression resulted frequently up-regulated in CRC patient tissue, and high expression of LINC00657 was correlated with an advanced clinical stage, lymph node metastasis, distant metastasis and poor overall survival of CRC patients. Furthermore, LINC00657 worked as a competing endogenous RNA (ceRNA) for miR-203a, antagonizing its function as a tumor suppressor and leading to the de-repression of CSCs invasion. Collectively, our observations revealed that LINC00657 is involved in CRC invasion by acting as a competing endogenous RNA. Thus, LINC00657 may serve as a potential prognostic factor and/or therapeutic target for CRC. more...
- Published
- 2020
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67. Identification of four Ferroptosis Gene signatures and Establishment of a predictive model for the overall survival rate of gastric cancer
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Jiang Liu, Hua-kai Tian, Nan-tao Fu, Ce-gui Hu, Fei-long Zou, Zhen Zong, and Zhi-kun Ning
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Background: Gastric cancer (GC) is highly aggressive and recurrent. Ferroptosis is believed to be closely related to the occurrence and development of tumors, but the specific mechanism is still unclear. This study aims to construct a new prognostic model based on ferroptosis-related gene scores to assess the prognosis of GC patients and guide clinical treatment.Materials and Methods: The gene expression information of GC patients with clinical data were obtained from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). Differentially expressed genes (DEGs) between tumor and normal tissue were screened from TCGA dataset, and ferroptosis-related genes were downloaded from the ferroptosis database. The ferroptosis-related DEGs were identified by intersecting the ferroptosis-related genes with DEGs. Univariate Cox and LASSO regression analysis was applied to identify survival-related intersection genes (SRIGs) and 4 hub genes. The TCGA dataset was randomly divided into 2 cohorts in a ratio of 0.7:0.3, the training cohort for construction of signature and the testing cohort for internal validation. The GSE26901 was used for external validation. Receiver operating characteristic (ROC), Kaplan-Meier curve and risk curve of 3 cohorts were plotted to evaluate performance of the signature. Multivariate Cox regression analysis was used to determine independent prognostic factors for gastric cancer, and then a prognostic nomogram was established based on risk score and clinicopathological parameters.Results: A four-gene signature comprising MYB, CHAC1, NOX4 and AFT3 was constructed to predict overall survival of GC. Using the best cut-off value to divide patients into high-risk and low-risk groups, we found that the survival rate of the low-risk group in the training group, test group and external validation group was significantly higher than that of the high-risk group (PConclusion: Our study identified a signature with 4 ferroptosis-related genes and established a reliable prognostic nomogram for predicting the overall survival rate of GC patients. The results may help medical decision-making, and provide a novel reference for predicting the prognosis of GC and even potential molecular targets. more...
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- 2022
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68. Novel aerosol detection platform for SARS‑CoV‑2: Based on specific magnetic nanoparticles adsorption sampling and digital droplet PCR detection
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Hui Chen, Xinye Ma, Xinyu Zhang, Gui Hu, Yan Deng, Song Li, Zhu Chen, Nongyue He, Yanqi Wu, and Zhihong Jiang
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General Chemistry - Abstract
The SARS‑CoV‑2 virus is released from an infectious source (such as a sick person) and adsorbed on aerosols, which can form pathogenic microorganism aerosols, which can affect human health through airborne transmission. Efficient sampling and accurate detection of microorganisms in aerosols are the premise and basis for studying their properties and evaluating their hazard. In this study, we built a set of sub-micron aerosol detection platform, and carried out a simulation experiment on the SARS‑CoV‑2 aerosol in the air by wet-wall cyclone combined with immunomagnetic nanoparticle adsorption sampling and ddPCR. The feasibility of the system in aerosol detection was verified, and the influencing factors in the detection process were experimentally tested. As a result, the sampling efficiency was 29.77%, and extraction efficiency was 98.57%. The minimum detection limit per unit volume of aerosols was 250 copies (10 more...
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- 2022
69. Research progress of severe acute respiratory syndrome coronavirus 2 on aerosol collection and detection
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Xinyu Zhang, Yuting Chen, Yueying Pan, Xinye Ma, Gui Hu, Song Li, Yan Deng, Zhu Chen, Hui Chen, Yanqi Wu, Zhihong Jiang, and Zhiyang Li
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General Chemistry - Published
- 2023
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70. Towards Identifying and Reducing the Bias of Disease Information Extracted from Search Engine Data.
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Da-Cang Huang, Jinfeng Wang 0001, Ji-Xia Huang, Daniel Z. Sui, Hongyan Zhang, Mao-Gui Hu, and Chengdong Xu
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- 2016
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71. The Hypoxic Landscape Stratifies Gastric Cancer Into 3 Subtypes With Distinct M6a Methylation and Tumor Microenvironment Infiltration Characteristics
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Zhi-kun Ning, Ce-gui Hu, Jiang Liu, Hua-kai Tian, Zhong-lin Yu, Hao-nan Zhou, Hui Li, and Zhen Zong
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Stomach Neoplasms ,Immunology ,Tumor Microenvironment ,Immunology and Allergy ,Humans ,Hypoxia ,Prognosis ,Methylation - Abstract
The interaction between hypoxia and RNA N6-methyladenosine (m6A) is an emerging focus of investigation. However, alterations in m6A modifications at distinct hypoxia levels remain uncharacterized in gastric cancer (GC). Unsupervised hierarchical clustering was performed to stratify samples into different clusters. Differentially expressed gene analysis, univariate Cox proportional hazards regression analysis, and hazard ratio calculations were used to establish an m6A score to quantify m6A regulator modification patterns. After using an algorithm integrating Least absolute shrinkage and selection operator (LASSO) and bootstrapping, we identified the best candidate predictive genes. Thence, we established an m6A-related hypoxia pathway gene prognostic signature and built a nomogram to evaluate its predictive ability. The area under the curve (AUC) value of the nomogram was 0.811, which was higher than that of the risk score (AUC=0.695) and stage (AUC=0.779), suggesting a high credibility of the nomogram. Furthermore, the clinical response of anti-PD-1/CTLA-4 immunotherapy between high- and low-risk patients showed a significant difference. Our study successfully explored a brand-new GC pathological classification based on hypoxia pathway genes and the quantification of m6A modification patterns. Comprehensive immune analysis and validation demonstrated that hypoxia clusters were reliable, and our signature could provide a new approach for clinical decision-making and immunotherapeutic strategies for GC patients. more...
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- 2022
72. Self-Healing, Injectable Hydrogel Based on Dual Dynamic Covalent Cross-Linking Against Postoperative Abdominal Cavity Adhesion
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Huihui Zeng, Xi Liu, Zequn Zhang, Xianwen Song, Jun Quan, Jun Zheng, Zhaolong Shen, Yaqiong Ni, Chuntai Liu, Yi Zhang, and Gui Hu
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Chitosan ,Biomedical Engineering ,Hydrogels ,Tissue Adhesions ,General Medicine ,Biochemistry ,Antioxidants ,Anti-Bacterial Agents ,Rats ,Biomaterials ,Delayed-Action Preparations ,Quality of Life ,Animals ,Quercetin ,Peritoneum ,Molecular Biology ,Biotechnology - Abstract
Clinically, increasing the peritoneal barrier is an effective adjunct to reducing postoperative peritoneal adhesion. This study presents a facile template for preparing a supramolecular hybrid hydrogel through dynamic covalent cross-linking between carboxymethyl chitosan (CMCS), 2-formylphenylboronic acid (2-FPBA), and quercetin (Que). The as-prepared complex CMCS/2-FPBA/Que (CFQ) hydrogel exhibited favorable antibacterial, anti-inflammatory, and antioxidant effects. A L929 cytotoxicity evaluation confirmed the favorable cytocompatibility of the CFQ hydrogel. The postoperative anti-adhesion ability of the CFQ hydrogel was further evaluated in rats with lateral wall defects and cecal abrasions. Compared with control groups, the tissue adhesion rate was significantly reduced by increasing the Que concentration in all the hydrogel-treated groups. Additionally, the sustained-release time of the C3F0.8Q0.08 hydrogel can exceed 14 days, which is highly desirable for clinical wound treatment. STATEMENT OF SIGNIFICANCE: Postoperative adhesions are a very common postoperative complication that seriously affects the quality of life of patients. The currently commonly used methods for preventing adhesion mainly use degradable barrier materials for physical separation. In this study, we prepared a dual dynamic covalently cross-linked CFQ hydrogel, which is not only degradable and injectable, but also has multiple properties such as antibacterial, antioxidant and anti-inflammatory, which can effectively prevent postoperative adhesion and promote wound healing. more...
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- 2022
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73. Metallothionein 2A (MT2A) controls cell proliferation and liver metastasis by controlling the MST1/LATS2/YAP1 signaling pathway in colorectal cancer
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Xi Liu, Jun Quan, Zhaolong Shen, Zequn Zhang, Zhijian Chen, Liang Li, Xiaorong Li, Gui Hu, and Xiaofeng Deng
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Cancer Research ,Oncology ,Genetics - Abstract
Background Colorectal cancer (CRC) is one of the three major cancers in the world and is the cancer with the most liver metastasis. The present study aimed to investigate the role of metallothionein 2A (MT2A) in the modulation of CRC cell proliferation and liver metastasis, as well as its molecular mechanisms. Methods The expression profile of metallothionein 2A (MT2A) in colorectal cancer retrieved from TCGA, GEO and Oncomine database. The biological effect of MT2A overexpression was investigated mainly involving cell proliferation and migration in CRC cells as well as growth and metastasis in CRC animal models. To explore the specific mechanism of MT2A metastasis in CRC, transcriptome sequencing was used to compare the overall expression difference between the control group and the MT2A overexpression group. Results Metallothionein 2A (MT2A) was downregulated in the tumor tissues of patients with CRC compared to adjacent normal tissues and was related to the tumor M stage of patients. MT2A overexpression inhibited CRC cell proliferation and migration in cells, as well as growth and metastasis in CRC animal models. While knockdown of MT2A had the opposite effect in cells. Western blotting confirmed that MT2A overexpression promoted the phosphorylation of MST1, LAST2 and YAP1, thereby inhibiting the Hippo signaling pathway. Additionally, specific inhibitors of MST1/2 inhibited MT2A overexpression-mediated phosphorylation and relieved the inhibition of the Hippo signaling pathway, thus promoting cell proliferation. Immunohistochemistry in subcutaneous grafts and liver metastases further confirmed this result. Conclusions Our results suggested that MT2A is involved in CRC growth and liver metastasis. Therefore, MT2A and MST1 may be potential therapeutic targets for patients with CRC, especially those with liver metastases. more...
- Published
- 2021
74. TRIM72-mediated degradation of the short form of p62/SQSTM1 rheostatically controls selective autophagy in human cells
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Cheng-Cheng Wang, Hong Peng, Zi Wang, Jiao Yang, Rong-Gui Hu, Chuan-Yin Li, and Wu-Jun Geng
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Sequestosome-1 Protein ,Autophagy ,Humans ,General Medicine ,Signal Transduction - Published
- 2021
75. Characteristics of two tsunamis generated by successive Mw 7.4 and Mw 8.1 earthquakes in Kermadec Islands on March 4, 2021
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Gui Hu, Kenji Satake, Mohammad Heidarzadeh, and Yuchen Wang
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Wavelet ,Ratio method ,Tide gauge ,Source spectrum ,Sea level ,Seismology ,Geology - Abstract
On March 4, 2021, two tsunamigenic earthquakes (Mw 7.4 and Mw 8.1) occurred successively within 2 h in Kermadec Islands. We examined sea level records at tide gauges located at ~100 km to ~2,000 km from the epicenters, conducted Fourier and Wavelet analyses as well as numerical modelling of both tsunamis. Fourier analyses indicated that the energy of the first tsunami is mainly distributed in the period range of 5–17 min, whereas it is 8–28 min for the second tsunami. Wavelet plots showed that the oscillation of the first tsunami continued even after the arrival of the second tsunami. As the epicenters of two earthquakes are close (~ 55 km), we reconstructed the source spectrum of the second tsunami by using the first tsunami as the empirical Green’s function. The main spectral peaks are 25.6 min, 16.0 min, and 9.8 min. The results are similar to those calculated using tsunami/background ratio method and also consistent with source models. more...
- Published
- 2021
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76. MiR-499a-5p promotes 5-FU resistance and the cell proliferation and migration through activating PI3K/Akt signaling by targeting PTEN in pancreatic cancer
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Liu Ouyang, Ren-Dong Liu, De-Qiao Lei, Qing-Chao Shang, Hui-Fen Li, Xian-Gui Hu, Hao Zheng, and Gang Jin
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Original Article ,General Medicine - Abstract
BACKGROUND: Pancreatic cancer (PC) can be considered a representative cancer type of the human body. As demonstrated by some studies, microRNA (miR)-499 is dysregulated in various cancer types including PC, for which chemotherapy involving 5-fluorouracil (5-FU) has long been considered the first-line therapy. However, there are complex and comprehensive mechanisms related to 5-FU, which have not been fully elucidated. This study thus aimed to examine the molecular mechanisms of 5-FU resistance through miR-499a-5p in PC. METHODS: The expression of miR-499a-5p in PC was measured using quantitative polymerase chain reaction (PCR). MiR-499a-5p was examined in-vivo for its effects on the malignant phenotypes of PC cells. RESULTS: The results of the present study demonstrated miR-499a-5p to be upregulated in PC and 5-FU resistant PC tissues. According to in vitro assays in PC cells (PANC1/FR), miR-499a-5p was found to affect adenosine triphosphate (ATP) binding cassette subfamily B member 1 (P-gp), ATP binding cassette subfamily C member 1 (MRP1), and ATP binding cassette subfamily G member 2 (BCRP), thereby facilitating 5-FU resistance in PC cells. Functions assays indicated that suppressed miR-499a-5p expression inhibited the proliferation and migration of cells but facilitated apoptosis in the PC cell line; by contrast, miR-499a-5p overexpression triggered the inverse phenotypic changes of cells. Concerning the mechanisms involved, miR-499a-5p increased PI3K/Akt signaling by targeting phosphatase and tensin homolog (PTEN). CONCLUSIONS: Taken together, these findings demonstrate that miR-499a-5p can be potentially applied to PC therapy. more...
- Published
- 2021
77. Circ_RNF13 Regulates the Stemness and Chemosensitivity of Colorectal Cancer by Transcriptional Regulation of DDX27 Mediated by TRIM24 Stabilization
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Yihang, Guo, Gui, Hu, Buning, Tian, Min, Ma, Fei, Long, and Miao, Chen
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Cancer Research ,Oncology ,circ_RNF13 ,DDX27 ,TRIM24 ,colorectal cancer - Abstract
Background: Colorectal cancer (CRC) is one of the most commonly diagnosed cancers with high incidence and poor prognosis worldwide. Circ_RNF13 is upregulated in CRC; however, the biological roles and downstream signaling of circ_RNF13 remain undefined. Methods: The characterization of circ_RNF13 was determined by Sanger sequencing, qRT-PCR, subcellular fractionation assay, and RNA FISH. Western blot analysis and qRT-PCR were employed to detect the expression of the key molecules and stemness markers in CRC tumor samples and cells. The stem-like activities of CRC cells were assessed by sphere formation assay, flow cytometry, and immunofluorescence (IF). Cell viability was monitored by CCK-8 assay. The chemosensitivity of CRC cells was assessed by colony formation and cell apoptosis assays. Bioinformatics analysis, RIP assay, RNA pull-down assay, and FISH/IF staining were used to detect the association between circ_RNF13 and TRIM24. The transcriptional regulation of DDX27 was investigated by ChIP assay, and the post-translational regulation of TRIM24 was detected by Co-IP. The in vitro findings were verified in a xenograft model. Results: circ_RNF13 and DDX27 were elevated in CRC tumor samples and cells. Knockdown of circ_RNF13 or DDX27 inhibited stemness and increased chemosensitivity in CRC cells. Mechanistically, circ_RNF13 regulated DDX27 expression via TRIM24-mediated transcriptional regulation, and circ_RNF13 stabilized TRIM24 via suppressing FBXW7-mediated TRIM24 degradation. In vivo studies revealed that the knockdown of circ_RNF13 impaired stemness and enhanced the chemosensitivity of CRC in the xenograft model. Conclusion: circ_RNF13 regulated the stemness and chemosensitivity of CRC by transcriptional regulation of DDX27 mediated by TRIM24 stabilization. more...
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- 2022
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78. Collaborative reverse logistics network for electric vehicle batteries management from sustainable perspective
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Gui Hu, Wenzhu Liao and Xuan, Luo
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Electric Power Supplies ,Environmental Engineering ,Electricity ,Waste Management ,Humans ,Recycling ,General Medicine ,Models, Theoretical ,Management, Monitoring, Policy and Law ,Waste Management and Disposal ,Carbon - Abstract
With the proposal of carbon neutralization, countries pay much more attention to environmental protection. As waste electric vehicle battery (WEVB) has an important impact on the environment, its reverse logistics process has been a vital issue, in which an excellent reverse logistics network (RLN) becomes a prerequisite for waste recycling, cost reduction, profit increasement and efficiency improvement. However, reverse logistics network design (RLND) for WEVBs is still a significant challenge. In general, the amount and quality of the returned product in a RLN are highly uncertain, and the WEVB market in China is no exception to this. From a circular economic and environmental perspective, this paper is devoted to designing a multi-participants-based RLN for WEVBs, considering recycling and remanufacturing processes. Then, a fuzzy optimization model is proposed to determine the number and location of facilities in this RLN, promote sustainable development, reduce environmental pollution, and consider carbon emissions and two types of WEVBs. Finally, the results from a case study in Chongqing show good performance in cost reduction, profit increasement and efficiency improvement, which could significantly support the decision-making of WEVBs recycling. more...
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- 2022
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79. A spatial sampling optimization package using MSN theory.
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Mao-Gui Hu and Jinfeng Wang 0001
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- 2011
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80. UformPose: A U-shaped Hierarchical Multi-Scale Keypoint-Aware Framework for Human Pose Estimation
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Wang, You-Jie, primary, Luo, Yan-Min, additional, Bai, Gui-Hu, additional, and Guo, Jing-Ming, additional
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- 2022
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81. The N
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Changwei, Lin, Min, Ma, Yi, Zhang, Liang, Li, Fei, Long, Canbin, Xie, Hua, Xiao, Teng, Liu, Buning, Tian, Kaiyan, Yang, Yihang, Guo, Miao, Chen, Jin, Chou, Ni, Gong, Xiaorong, Li, and Gui, Hu
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Adenosine ,Prostaglandins F ,RNA, Circular ,Gene Expression Regulation, Neoplastic ,Mice ,MicroRNAs ,Cell Movement ,Cell Line, Tumor ,Animals ,Humans ,Female ,Colorectal Neoplasms ,Cell Proliferation ,Placenta Growth Factor - Abstract
Previous studies have shown that the NWe used a human circRNA microarray to detect the expression profiles of circRNAs in 3 pairs of cancer and paracancerous tissues from patients with colorectal cancer (CRC) and 3 pairs of peripheral blood specimens from patients with CRC and healthy individuals. The circRNAs highly expressed in both peripheral blood and tumour tissues of patients with CRC, including circALG1, were screened. A quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis of an expanded sample size was performed to detect the expression level of circALG1 in peripheral blood and tumour tissues of patients with CRC and determine its correlation with clinicopathological features, and circRNA loop-forming validation and stability assays were then conducted. Transwell assays and a nude mouse cancer metastasis model were used to study the function of circALG1 in CRC and the role of altered mCircALG1 was highly expressed in both the peripheral blood and tumour tissues of patients with CRC and was closely associated with CRC metastasis. CircALG1 overexpression promoted the migration and invasion of CRC cells, and circALG1 silencing and reduction of the circALG1 mM more...
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- 2021
82. PTBP3 modulates P53 expression and promotes colorectal cancer cell proliferation by maintaining UBE4A mRNA stability
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Canbin Xie, Fei Long, Liang Li, Xiaorong Li, Min Ma, Zhixing Lu, Runliu Wu, Yi Zhang, Lihua Huang, Jing Chou, Ni Gong, Gui Hu, and Changwei Lin
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Cancer Research ,QH573-671 ,RNA Stability ,Ubiquitin-Protein Ligases ,Immunology ,Cell Biology ,Gene Expression Regulation, Neoplastic ,Cellular and Molecular Neuroscience ,Cell Line, Tumor ,Humans ,Tumor Suppressor Protein p53 ,Cytology ,Colorectal Neoplasms ,3' Untranslated Regions ,Cell Proliferation ,Polypyrimidine Tract-Binding Protein - Abstract
The RNA binding protein PTBP3 was recently reported to play a critical role in multiple cancers, and the molecular mechanisms involved RNA splicing, 3′ end processing and translation. However, the role of PTBP3 in colorectal cancer (CRC) remains poorly explored. Herein, PTBP3 was upregulated in CRC and associated with a poor prognosis. PTBP3 knockdown in colorectal cancer cell lines restricted CRC proliferative capacities in vitro and in vivo. Mechanistically, PTBP3 regulated the expression of the E3 ubiquitin ligase UBE4A by binding the 3′ UTR of its mRNA, preventing its degradation. UBE4A participated in P53 degradation, and PTBP3 knockdown in colorectal cancer cell lines showed increased P53 expression. UBE4A overexpression rescued PTBP3 knockdown-induced inhibition of CRC cell proliferation and P53 expression. Our results demonstrated that PTBP3 plays an essential role in CRC cell proliferation by stabilizing UBE4A to regulate P53 expression and may serve as a new prognostic biomarker and effective therapeutic target for CRC. more...
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- 2021
83. The Expression Profiles and Deregulation of UDP-Glycosyltransferase (UGT) Genes in Human Cancers and Their Association with Clinical Outcomes
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Dong Gui Hu, Ross A. McKinnon, Peter I. Mackenzie, Shashikanth Marri, Robyn Meech, and Julie-Ann Hulin
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UGT1A6 ,Cancer Research ,overall survival ,Biology ,prognostic biomarkers ,Genome ,digestive system ,Article ,UGT8 ,Gene expression ,medicine ,cancer ,differential gene expression ,Gene ,Carcinogen ,RC254-282 ,Cancer ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,drug metabolism ,Oncology ,Cancer research ,gene expression ,UDP-glycosyltransferase ,Drug metabolism ,UDP-glucuronosyltransferase - Abstract
The human UDP-glycosyltransferase (UGTs) superfamily has 22 functional enzymes that play a critical role in the metabolism of small lipophilic compounds, including carcinogens, drugs, steroids, lipids, fatty acids, and bile acids. The expression profiles of UGT genes in human cancers and their impact on cancer patient survival remains to be systematically investigated. In the present study, a comprehensive analysis of the RNAseq and clinical datasets of 9514 patients from 33 different TCGA (the Genome Cancer Atlas) cancers demonstrated cancer-specific UGT expression profiles with high interindividual variability among and within individual cancers. Notably, cancers derived from drug metabolizing tissues (liver, kidney, gut, pancreas) expressed the largest number of UGT genes (COAD, KIRC, KIRP, LIHC, PAAD), six UGT genes (1A6, 1A9, 1A10, 2A3, 2B7, UGT8) showed high expression in five or more different cancers. Kaplan–Meier plots and logrank tests revealed that six UGT genes were significantly associated with increased overall survival (OS) rates [UGT1A1 (LUSC), UGT1A6 (ACC), UGT1A7 (ACC), UGT2A3 (KIRC), UGT2B15 (BLCA, SKCM)] or decreased OS rates [UGT2B15 (LGG), UGT8 (UVM)] in specific cancers. Finally, differential expression analysis of 611 patients from 12 TCGA cancers identified 16 UGT genes (1A1, 1A3, 1A6, 1A7, 1A8, 1A9, 1A10, 2A1, 2A3, 2B4, 2B7, 2B11, 2B15, 3A1, 3A2, UGT8) that were up/downregulated in at least one cancer relative to normal tissues. In conclusion, our data show widespread expression of UGT genes in cancers, highlighting the capacity for intratumoural drug metabolism through the UGT conjugation pathway. The data also suggests the potentials for specific UGT genes to serve as prognostic biomarkers or therapeutic targets in cancers. more...
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- 2021
84. PTBP3 Modulates of P53 Expression and Promotes Colorectal Cancer Cell Proliferation by Maintaining UBE4A mRNA Stability
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Zhixing Lu, Yi Zhang, Ni Gong, Gui Hu, Canbin Xie, Fei Long, Min Ma, Runliu Wu, Changwei Lin, Lihua Huang, Chou Jing, Xiaorong Li, and Liang Li
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Messenger RNA ,Cell growth ,Colorectal cancer ,Cancer research ,medicine ,Biology ,medicine.disease ,P53 expression ,digestive system diseases - Abstract
Background Colorectal cancer (CRC) is the most common malignancy worldwide and has become the second leading cause of cancer-related death worldwide. The RNA-binding protein polypyrimidine tract-binding protein 3 (PTBP3) was recently reported to play a critical role in multiple cancers, and its molecular mechanisms involve RNA splicing, 3′ end processing and translation. However, the role of PTBP3 in CRC is unclear. Methods We analyzed the expression levels of PTPB3 using The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets and clinical tissues. The effect of PTBP3 on CRC cell proliferation was measured by Cell Counting Kit-8 (CCK-8), colony formation, flow cytometry and tumor xenograft assays. A series of experiments were conducted to reveal the mechanisms by which PTBP3 promotes CRC proliferation. Results We showed that PTBP3 was upregulated in CRC and associated with a poor prognosis. Knockdown of PTBP3 in CRC cell lines restricted CRC proliferative capacities in vitro and in vivo. Mechanistically, we found that PTBP3 regulated the expression of the E3 ubiquitin ligase ubiquitination factor E4A (UBE4A) by binding the 3' untranslated region (UTR) of its mRNA, thereby preventing its degradation. We also discovered that UBE4A participated in the degradation of P53, and knockdown of PTBP3 in CRC cell lines increased P53 expression. Overexpression of UBE4A rescued PTBP3 knockdown-induced inhibition of CRC cell proliferation and P53 expression. Conclusions PTBP3 plays an essential role in CRC cell proliferation by stabilizing UBE4A to regulate P53 expression and may serve as a new prognostic biomarker and effective therapeutic target for CRC. more...
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- 2021
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85. [Effects of Microplastics Addition on Soil Organic Carbon Mineralization in Citrus Orchard]
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Xiu-Ling, Zhang, Zi-Wei, Yan, Feng, Wang, Xi, Wang, Han, Xu, Rong-Gui, Hu, Chang, Yan, and Shan, Lin
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Citrus ,Soil ,Microplastics ,Plastics ,Carbon - Abstract
To investigate the effects of microplastics on soil organic carbon mineralization and the changes in soil enzyme activities, an incubation experiment was conducted whereby single applications of either microplastics or straw, and combined application of both, were added to Dangyang citrus orchard soil. The results showed that the combined application of straw and microplastics significantly affected organic carbon mineralization in the soil, but the single addition of microplastics had no significant effect. Compared with straw alone, the application of a small combined amount of microplastics and straw significantly increased soil organic carbon mineralization by 8.20%, while medium and high amounts of the combined application significantly inhibited soil organic carbon mineralization. The lowest amount of organic carbon mineralization occurred with the highest amount of combined microplastics and straw, 10.13% lower than with straw alone. The addition of microplastics significantly reduced the activity of more...
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- 2021
86. Theoretical and extraction studies on the selectivity of lithium with 14C4 derivatives
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Liang Xu, Yongpan Tian, Cheng-Gui Hu, Zhuo Zhao, Bihai Tong, and Wenwen Chen
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Chemistry ,Metal ions in aqueous solution ,Atoms in molecules ,chemistry.chemical_element ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Dispersion (geology) ,01 natural sciences ,Catalysis ,0104 chemical sciences ,Metal ,Adsorption ,visual_art ,Selective adsorption ,Materials Chemistry ,visual_art.visual_art_medium ,Physical chemistry ,Density functional theory ,Lithium ,0210 nano-technology - Abstract
Lithium is a critical strategic metal for world economy development and is used in various fields, such as daily life, aviation, medicine, chemical industry, etc. Crown ethers can adsorb Li+ from a mixed ionic solution based on the size matching effect and synergistic effects of functional groups. The selective adsorption properties with Li+, Na+, Mg2+ and the interaction between crown ethers and metal ions were analyzed to guide the design of crown ethers with high selectivity for Li+. The geometric structural characteristics of 1,8-dihydroxyl-4,4,5,5-tetramethylbenzo-14-crown-4 (CE) and complexes with Li+, Na+, and Mg2+ metal ions were investigated using density functional theory modeling (DFT) at the M062X/def2SVP, def2TZVP level. The nature and strength of the interactions were analyzed by atoms in molecules (AIM) topological analysis and symmetry-adapted perturbation theory (SAPT) energy decomposition analysis. The results showed that the interaction strength of CE with metal ions followed the order: CE-Mg2+ > CE-Li+ > CE-Na+. The interaction energies can be separated into four kinds: electrostatics, exchange, induction, and dispersion. The stability of these complexes was mainly driven by electrostatics and induction. According to the analysis results of reduced density gradient (RDG), the metal ions mainly interacted with the oxygen atoms on the ring and did not interact with the hydroxyl groups directly. CE was synthesized and the extraction rates of Mg2+ and Li+ were better than that of Na+. more...
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- 2020
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87. The UDP-Glycosyltransferase (UGT) Superfamily: New Members, New Functions, and Novel Paradigms
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Dong Gui Hu, Siti Nurul Mubarokah, Robyn Meech, Peter I. Mackenzie, Pramod C. Nair, Ross A. McKinnon, Andrew Rowland, and Alex Z. Haines
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Mammals ,0301 basic medicine ,Physiology ,Chemistry ,Glycosyltransferases ,SUPERFAMILY ,General Medicine ,Gene Expression Regulation, Enzymologic ,Udp glycosyltransferase ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Biochemistry ,Biotransformation ,Multigene Family ,030220 oncology & carcinogenesis ,Physiology (medical) ,Animals ,Molecular Biology ,Signal Transduction - Abstract
UDP-glycosyltransferases (UGTs) catalyze the covalent addition of sugars to a broad range of lipophilic molecules. This biotransformation plays a critical role in elimination of a broad range of exogenous chemicals and by-products of endogenous metabolism, and also controls the levels and distribution of many endogenous signaling molecules. In mammals, the superfamily comprises four families: UGT1, UGT2, UGT3, and UGT8. UGT1 and UGT2 enzymes have important roles in pharmacology and toxicology including contributing to interindividual differences in drug disposition as well as to cancer risk. These UGTs are highly expressed in organs of detoxification (e.g., liver, kidney, intestine) and can be induced by pathways that sense demand for detoxification and for modulation of endobiotic signaling molecules. The functions of the UGT3 and UGT8 family enzymes have only been characterized relatively recently; these enzymes show different UDP-sugar preferences to that of UGT1 and UGT2 enzymes, and to date, their contributions to drug metabolism appear to be relatively minor. This review summarizes and provides critical analysis of the current state of research into all four families of UGT enzymes. Key areas discussed include the roles of UGTs in drug metabolism, cancer risk, and regulation of signaling, as well as the transcriptional and posttranscriptional control of UGT expression and function. The latter part of this review provides an in-depth analysis of the known and predicted functions of UGT3 and UGT8 enzymes, focused on their likely roles in modulation of levels of endogenous signaling pathways. more...
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- 2019
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88. Spatiotemporal interpolation of precipitation across Xinjiang, China using space-time CoKriging
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Dan-gui Hu and Hong Shu
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Multivariate statistics ,010504 meteorology & atmospheric sciences ,Correlation coefficient ,Mean squared error ,Metals and Alloys ,General Engineering ,01 natural sciences ,010104 statistics & probability ,Kriging ,Covariate ,Statistics ,Statistics::Methodology ,Environmental science ,Relative humidity ,Precipitation ,0101 mathematics ,0105 earth and related environmental sciences ,Interpolation - Abstract
In various environmental studies, geoscience variables not only have the characteristics of time and space, but also are influenced by other variables. Multivariate spatiotemporal variables can improve the accuracy of spatiotemporal estimation. Taking the monthly mean ground observation data of the period 1960–2013 precipitation in the Xinjiang Uygur Autonomous Region, China, the spatiotemporal distribution from January to December in 2013 was respectively estimated by space-time Kriging and space-time CoKriging. Modeling spatiotemporal direct variograms and a cross variogram was a key step in space-time CoKriging. Taking the monthly mean air relative humidity of the same site at the same time as the covariates, the spatiotemporal direct variograms and the spatiotemporal cross variogram of the monthly mean precipitation for the period 1960–2013 were modeled. The experimental results show that the space-time CoKriging reduces the mean square error by 31.46% compared with the space-time ordinary Kriging. The correlation coefficient between the estimated values and the observed values of the space-time CoKriging is 5.07% higher than the one of the space-time ordinary Kriging. Therefore, a space-time CoKriging interpolation with air humidity as a covariate improves the interpolation accuracy. more...
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- 2019
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89. [Spatiotemporal Distribution of Aerosol Optical Depth Based on Landsat Data in the Hinterland of the Guanzhong Basin and Its Relationship with Urbanization]
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Yu-Rong, Zheng, Xu-Hong, Wang, Xiu, Zhang, Gui-Gui, Hu, Xiu-Juan, Liang, Lin-Zhi, Niu, and Hai-Qing, Han
- Abstract
Aerosol optical depth (AOD) is one of the most fundamental optical properties of aerosols that characterizes the attenuation of light by aerosols and is an indicator of regional air pollution. Based on the blue band surface reflectance database from the MOD09A1 products for the period 2000-2019 and the ASTER spectral database, AOD was estimated from Landsat TM/OLI data using the deep blue algorithm (DB). Multi-year average/annual average and seasonal AOD values for the period 2000-2019 were then calculated to analyze the spatial characteristics and temporal variations of AOD using the empirical orthogonal function method (EOF). Furthermore, the impacts of urbanization on the spatio-temporal distribution of AOD were analyzed. The obtained results are summarized as follows:① The multi-year average AOD spatial distribution in the hinterland of the Guanzhong Basin was significantly affected by topography and human activities, with higher AOD values and variationsin areas of low altitude and high-intensity human activities compared to the surrounding mountains. Thus, changes in AOD in the study area are mainly affected by anthropogenic factors. AOD also showed significant seasonal variations, whereby spring (0.34)summer (0.33)autumn (0.23)winter (0.12), and the largest regional differences occurred in summer; ② The annual average AOD (from 2000-2019) showed the trend of "increase-decrease-increase", and reached a maximum in 2005, with the high AOD area gradually moving to the south. The distribution of AOD values in spring and summer was relatively discrete, while it is in a low-value agglomeration state in winter; ③ Three main AOD spatial distribution modes were identified based on the EOF, which had cumulative contribution rate of 83.0%. The spatial distribution trend of AOD showed regional consistency, with feature vectors consistent with the altitude, thus reflecting the difference of AOD at different altitudes. Taking the Qinling Mountains as the dividing line, the AOD presented the "north-south" pattern, AOD showed a "north-south" pattern, reflecting the uniqueness of the regional development in the Guanzhong Basin compared to the southern Qinling Mountains. The "southeast-northwest" distribution pattern indicated that the AOD presented a reverse change trend between urban and non-urban; and ④ The results of correlation analysis between the AOD and urbanization revealed a positive correlation with permanent population density ( more...
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- 2021
90. Nomogram predicts CRPOPF in open central pancreatectomy patients with benign or low-grade malignant pancreatic neoplasms .
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Liu Ouyang, Ren-dong Liu, Yi-wei Ren, Gang Nie, Tian-lin He, Gang Li, Ying-qi Zhou, Zhi-ping Huang, Yi-jie Zhang, Xian-gui Hu, and Gang Jin
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PANCREATECTOMY ,NOMOGRAPHY (Mathematics) ,SURGICAL site infections ,TUMORS ,PANCREATIC duct ,PANCREATIC fistula - Abstract
Introduction: Central pancreatectomy (CP) is a standard surgical procedure for benign and low-grade malignant pancreatic neoplasms in the body and neck of the pancreas. Higher incidence of clinically relevant postoperative pancreatic fistula (CR-POPF) after CP than after pancreaticoduodenectomy (PD) or distal pancreatectomy (DP) has been reported, but no nomogram for prediction of CR-POPF after open CP has been previously established. Methods: Patients undergoing open CP for benign or low-grade malignant pancreatic neoplasms in the department of Hepatobiliary and Pancreatic (HBP) surgery of Shanghai Changhai Hospital affiliated to Naval Medical University between January 01, 2009 and December 31,2020 were enrolled. Pre-, intraand post-operative parameters were analyzed retrospectively. Results: A total of 194 patients, including 60 men and 134 women, were enrolled with median age of 52 years (21~85 years). 84 patients (43.3%) were overweight (BMI>23.0 Kg/m2) and 14 (7.2%) were obese (BMI>28.0 Kg/m2). Pathological diagnoses ranged from serous cystic neoplasm (32.5%), solid pseudopapillary neoplasm (22.2%), pancreatic neuroendocrine tumor (20.1%), intraductal papillary mucinous neoplasm (18.0%) to mucinous cystic neoplasm (5.2%). All patients had soft pancreatic texture. Main pancreatic duct diameters were ≤0.3cm for 158 patients (81.4%) and were ≥0.5cm in only 12 patients (6.2%). A stapler (57.7%) or hand-sewn closure (42.3%) were used to close the pancreatic remnant. The pancreatic anastomosis techniques used were duct to mucosa pancreaticojejunostomy (PJ)-interrupted suture (47.4%), duct to mucosa PJ-continuous suture (43.3%), duct to mucosa “HO” half-purse binding PJ (5.2%) and invaginating pancreaticogastrostomy (4.1%). Postsurgical incidences of CR-POPF of 45.9%, surgical site infection of 28.9% postpancreatectomy hemorrhage of 7.7% and delayed gastric emptying of 2.1% were found. Obesity and pancreatic anastomosis technique were independent risk factors of CR-POPF, with a concordance index of 0.675 and an Area Under the Curve of 0.678. Discussion: This novel nomogram constructed according to obesity and pancreatic anastomosis technique showed moderate predictive performance of CR-POPF after open CP. [ABSTRACT FROM AUTHOR] more...
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- 2022
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91. OmicShare tools: A zero‐code interactive online platform for biological data analysis and visualization
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Hongyan Mu, Jianzhou Chen, Wenjie Huang, Gui Huang, Meiying Deng, Shimiao Hong, Peng Ai, Chuan Gao, and Huangkai Zhou
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Computer applications to medicine. Medical informatics ,R858-859.7 - Published
- 2024
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92. Adaptive node feature extraction in graph-based neural networks for brain diseases diagnosis using self-supervised learning
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Youbing Zeng, Jiaying Lin, Zhuoshuo Li, Zehui Xiao, Chen Wang, Xinting Ge, Cheng Wang, Gui Huang, and Mengting Liu
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Self-supervised learning ,Graph neural network ,Adaptive node feature ,Brain diseases ,EEG ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Electroencephalography (EEG) has demonstrated significant value in diagnosing brain diseases. In particular, brain networks have gained prominence as they offer additional valuable insights by establishing connections between EEG signal channels. While brain connections are typically delineated by channel signal similarity, there lacks a consistent and reliable strategy for ascertaining node characteristics. Conventional node features such as temporal and frequency domain properties of EEG signals prove inadequate for capturing the extensive EEG information. In our investigation, we introduce a novel adaptive method for extracting node features from EEG signals utilizing a distinctive task-induced self-supervised learning technique. By amalgamating these extracted node features with fundamental edge features constructed using Pearson correlation coefficients, we showed that the proposed approach can function as a plug-in module that can be integrated to many common GNN networks (e.g., GCN, GraphSAGE, GAT) as a replacement of node feature selections module. Comprehensive experiments are then conducted to demonstrate the consistently superior performance and high generality of the proposed method over other feature selection methods in various of brain disorder prediction tasks, such as depression, schizophrenia, and Parkinson’s disease. Furthermore, compared to other node features, our approach unveils profound spatial patterns through graph pooling and structural learning, shedding light on pivotal brain regions influencing various brain disorder prediction based on derived features. more...
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- 2024
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93. Cardioprotective effect of ginsenoside Rb1 via regulating metabolomics profiling and AMP-activated protein kinase-dependent mitophagy
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Lu Zhang, Boyang Yu, Fei Fu, Ling Zhang, Fang Li, Junping Kou, Qiong Lai, Jin-Gui Hu, and Tao Liu
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biology ,Chemistry ,AMPK ,Endogeny ,Pharmacology ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,eye diseases ,Ginseng ,Metabolomics ,Complementary and alternative medicine ,AMP-activated protein kinase ,Mitophagy ,biology.protein ,Phosphorylation ,Protein kinase A ,Biotechnology - Abstract
Background Ginsenoside Rb1, a bioactive component isolated from the Panax ginseng, acts as a remedy to prevent myocardial injury. However, it is obscure whether the cardioprotective functions of Rb1 are related to the regulation of endogenous metabolites, and its potential molecular mechanism still needs further clarification, especially from a comprehensive metabolomics profiling perspective. Methods The mice model of acute myocardial ischemia (AMI) and oxygen glucose deprivation (OGD)-induced cardiomyocytes injury were applied to explore the protective effect and mechanism of Rb1. Meanwhile, the comprehensive metabolomics profiling was conducted by high-performance liquid chromatography and quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS) and a tandem liquid chromatography and mass spectrometry (LC-MS). Results Rb1 treatment profoundly reduced the infarct size and attenuated myocardial injury. The metabolic network map of 65 differential endogenous metabolites was constructed and provided a new inspiration for the treatment of AMI by Rb1, which was mainly associated with mitophagy. In vivo and in vitro experiments, Rb1 was found to improve mitochondrial morphology, mitochondrial function and promote mitophagy. Interestingly, the mitophagy inhibitor partly attenuated the cardioprotective effect of Rb1. Additionally, Rb1 markedly facilitated the phosphorylation of AMP-activated protein kinase α (AMPKα), and AMPK inhibition partially weakened the role of Rb1 in promoting mitophagy. Conclusions Ginsenoside Rb1 protects acute myocardial ischemia injury through promoting mitophagy via AMPKα phosphorylation, which might lay the foundation for the further application of Rb1 in cardiovascular diseases. more...
- Published
- 2021
94. The source characteristics and tsunami resonance effect of the 2020 Samos earthquake in Eastern Aegean
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Gui Hu, Xiaohui He, Linlin Li, Cagil Karakas, Wanpeng Feng, Yunfeng Tian, and Yuchen Wang
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Geology ,Resonance effect ,Seismology - Abstract
On October 30 2020 11:51 UTC, a Mw 6.9 normal fault earthquake occurred off the northern coasts of Samos Island, Eastern Aegean, Greece. Over a 120 people were killed and more than 1000 people were injured during the seismic sequence. The quake produced a moderate tsunami that swapped the coastal areas of Izmir (Turkey) and Samos (Greece) with inundation heights up to ~3 m. Finding the source of such a tsunami has been puzzling as a normal fault earthquake with Mw 6.9 would not be considered significant enough to generate metric-scale waves. Furthermore, the lack of near-field observations has made the identification of the seismogenic fault responsible for the mainshock difficult. In this study, we infer the source characteristics from multiple observation data, including InSAR, GPS, teleseismic waves and tsunami waves. We first generate two Sentinel-1 co-seismic interferograms with a maximum Line of Sight (LOS) change of 8 cm on the coastal areas at the Samos island. We obtain a north-dipping fault model, which can slightly better explain the geodetic observations and teleseismic P waves. To understand the potential tsunami source, we use several earthquake slip models collected from different research groups to conduct tsunami simulations. Comparing simulated tsunami waveforms with those measured at 6 local tide gauges, we show that the north-dipping fault can fit tsunami records better than the south-dipping fault. The north-dipping fault hypothesis is also further supported by the spatial distributions of the aftershocks. The spectral analysis of tsunami waveforms at selected tide gauges suggests that the tsunami period band is within 4.6 ~ 21.3 min and the primary wave period is ~14.2 min. Using this wave period as an indirect constraint, we show that the source dimension of our slip model can produce tsunami waveforms with similar wave period. We also find high-energy wave of the Samos earthquake that lasted 20 h, and fundamental oscillation periods of Sığacık Bay are remarkably close to some dominating tsunami periods. We infer the coseismic seafloor displacement alone is not enough to create disastrous effects on coastal cities; therefore we suggest that the tsunami waves may have been amplified by local coastline and tsunami resonance with local bay, or another source, e.g. triggered landslides. more...
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- 2021
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95. COL5A1 Promotes the Progression of Gastric Cancer by Acting as a ceRNA of miR-137-3p to Upregulate FSTL1 Expression
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Ming Yang, Zhixing Lu, Bowen Yu, Jiajia Zhao, Liang Li, Kaiyu Zhu, Min Ma, Fei Long, Runliu Wu, Gui Hu, Lihua Huang, Jing Chou, Ni Gong, Kaiyan Yang, Xiaorong Li, Yi Zhang, and Changwei Lin
- Subjects
Cancer Research ,Oncology ,bioinformatics ,COL5A1 ,miR-137-3p ,FSTL1 ,gastric cancer ,immune infiltration - Abstract
MicroRNAs (miRNAs) and their target genes have been shown to play an important role in gastric cancer but have not been fully clarified. Therefore, our goal was to identify the key miRNA–mRNA regulatory network in gastric cancer by utilizing a variety of bioinformatics analyses and experiments. A total of 242 miRNAs and 1080 genes were screened from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO), respectively. Then, survival-related differentially expressed miRNAs and their differentially expressed target genes were screened. Twenty hub genes were identified from their protein–protein interaction network. After weighted gene co-expression network analysis was conducted, we selected miR-137-3p and its target gene, COL5A1, for further research. We found that miR-137-3p was significantly downregulated and that overexpression of miR-137-3p suppressed the proliferation, invasion, and migration of gastric cancer cells. Furthermore, we found that its target gene, COL5A1, could regulate the expression of another hub gene, FSTL1, by sponging miR-137-3p, which was confirmed by dual-luciferase reporter assays. Knockdown of COL5A1 inhibited the proliferation, invasion, and migration of gastric cancer cells, which could be rescued by the miR-137-3p inhibitor or overexpression of FSTL1. Ultimately, bioinformatics analyses showed that the expression of FSTL1 was highly correlated with immune infiltration. more...
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- 2022
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96. LINC01094 promotes pancreatic cancer progression by sponging miR-577 to regulate LIN28B expression and the PI3K/AKT pathway
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Jinfeng Zhu, Ce-Gui Hu, Kang Lin, Xiaojian Zhu, Chen Luo, and Zhengming Zhu
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LIN28B ,proliferation ,pancreatic cancer ,Cancer metastasis ,Endogeny ,RM1-950 ,miR-577 ,medicine.disease_cause ,Metastasis ,Downregulation and upregulation ,Pancreatic cancer ,Drug Discovery ,medicine ,metastasis ,PI3K/AKT/mTOR pathway ,business.industry ,medicine.disease ,PI3K/AKT pathway ,Cancer research ,Molecular Medicine ,Biomarker (medicine) ,Original Article ,Therapeutics. Pharmacology ,Carcinogenesis ,business ,LINC01094 - Abstract
The leading cause of death in pancreatic cancer (PC) patients is the progression of cancer metastasis. Recently, long non-coding RNAs (lncRNAs) have been shown to play an important role in regulating cancer cell proliferation and metastasis; however, its molecular basis in PC remains to be explored. In this study, we observed that LINC01094 was markedly overexpressed in PC tissues and was associated with poor patient prognosis. Downregulation of LINC01094 decreased the proliferation and metastasis of PC cells and inhibited tumorigenesis and metastasis in mouse xenografts. Mechanically, LINC01094 acted as an endogenous miR-577 sponge to increase the expression of its target gene, the RNA-binding protein lin-28 homolog B (LIN28B), by decoying the miR-577, thereby activating the PI3K/AKT pathway. Our findings suggest that LINC01094 plays critical roles in proliferation and metastasis of PC, implying that LINC01094 can be regarded as a new biomarker or therapeutic target for the treatment of PC., Graphical abstract, LINC01094 was markedly overexpressed in PC tissues and was associated with poor patient prognosis. Downregulation of LINC01094 decreased the proliferation and metastasis of pancreatic cancer cells. Mechanically, LINC01094 acted as an endogenous miR-577 sponge to increase LIN28B by decoying the miR-577, resulting in PI3K/AKT pathway activation. more...
- Published
- 2021
97. Circular RNAs of UDP-Glycosyltransferase (
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Dong Gui, Hu, Peter I, Mackenzie, Julie-Ann, Hulin, Ross A, McKinnon, and Robyn, Meech
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Alternative Splicing ,Cell Line, Tumor ,Humans ,RNA, Circular ,Cloning, Molecular ,Glucuronosyltransferase ,Transcriptome - Abstract
The human UDP-glycosyltransferase (
- Published
- 2020
98. Activation of P2×7 Receptor Promotes the Invasion and Migration of Colon Cancer Cells via the STAT3 Signaling
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Wen-Jun Zhang, Hong-liang Luo, Zheng-Ming Zhu, and Ce-Gui Hu
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0301 basic medicine ,Colorectal cancer ,Vimentin ,invasion and migration ,Calcium in biology ,Metastasis ,Pathogenesis ,03 medical and health sciences ,Cell and Developmental Biology ,0302 clinical medicine ,In vivo ,medicine ,STAT3 ,Receptor ,lcsh:QH301-705.5 ,Original Research ,biology ,P2 × 7 receptor ,Chemistry ,Cell Biology ,medicine.disease ,ATP ,LOVO and SW480 cells ,030104 developmental biology ,lcsh:Biology (General) ,colon cancer ,030220 oncology & carcinogenesis ,Cancer research ,biology.protein ,Developmental Biology - Abstract
The pathological mechanism of colon cancer is very complicated. Therefore, exploring the molecular basis of the pathogenesis of colon cancer and finding a new therapeutic target has become an urgent problem to be solved in the treatment of colon cancer. ATP plays an important role in regulating the progression of tumor cells. P2 × 7 belongs to ATP ion channel receptor, which is involved in the progression of tumors. In this study, we explored the effect and molecular mechanism of ATP-mediated P2 × 7 receptor on the migration and metastasis of colon cancer cells. The results showed that ATP and BzATP significantly increased the inward current and intracellular calcium concentration of LOVO and SW480 cells, while the use of antagonists (A438079 and AZD9056) could reverse the above phenomenon. We found that ATP promoted the migration and invasion of LOVO and SW480 cells and is dose-dependent on ATP concentration (100–300 μM). Similarly, BzATP (10, 50, and 100 μM) also significantly promoted the migration and invasion of colon cancer cells in a concentration-dependent manner. While P2 × 7 receptor antagonists [A438079 (10 μM), AZD9056 (10 μM)] or P2 × 7 siRNA could significantly inhibit ATP-induced colon cancer cell migration and invasion. Moreover,in vivoexperiments showed that ATP-induced activation of P2 × 7 receptor promoted the growth of tumors. Furthermore, P2 × 7 receptor activation down-regulated E-cadherin protein expression and up-regulated MMP-2 mRNA and concentration levels. Knocking down the expression of P2 × 7 receptor could significantly inhibit the increase in the expression of N-cadherin, Vimentin, Zeb1, and Snail induced by ATP. In addition, ATP time-dependently induced the activation of STAT3 via the P2 × 7 receptor, and the STAT3 pathway was required for the ATP-mediated invasion and migration. Our conclusion is that ATP-induced P2 × 7 receptor activation promotes the migration and invasion of colon cancer cells, possibly via the activation of STAT3 pathway. Therefore, the P2 × 7 receptor may be a potential target for the treatment of colon cancer. more...
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- 2020
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99. The Expression Profiles of ADME Genes in Human Cancers and Their Associations with Clinical Outcomes
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Ross A. McKinnon, Peter I. Mackenzie, Pramod C. Nair, Dong Gui Hu, and Robyn Meech
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0301 basic medicine ,Cancer Research ,CYP2D6 ,Abcg2 ,overall survival ,endobiotic metabolism ,CYP2C19 ,lcsh:RC254-282 ,Article ,03 medical and health sciences ,GSTP1 ,0302 clinical medicine ,cancer ,CYP3A5 ,CYP2C8 ,prognostic biomarker ,ADME ,biology ,drug absorption ,therapeutic target ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,drug metabolism ,drug distribution ,030104 developmental biology ,Oncology ,drug excretion ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,biology.protein - Abstract
ADME genes are a group of genes that are involved in drug absorption, distribution, metabolism, and excretion (ADME). The expression profiles of ADME genes within tumours is proposed to impact on cancer patient survival, however, this has not been systematically examined. In this study, our comprehensive analyses of pan-cancer datasets from the Cancer Genome Atlas (TCGA) revealed differential intratumoral expression profiles for ADME genes in 21 different cancer types. Most genes also showed high interindividual variability within cancer-specific patient cohorts. Using Kaplan-Meier plots and logrank tests, we showed that intratumoral expression levels of twenty of the thirty-two core ADME genes were associated with overall survival (OS) in these cancers. Of these genes, five showed significant association with unfavourable OS in three cancers, including SKCM (ABCC2, GSTP1), KIRC (CYP2D6, CYP2E1), PAAD (UGT2B7), sixteen showed significant associations with favourable OS in twelve cancers, including BLCA (UGT2B15), BRCA (CYP2D6), COAD (NAT1), HNSC (ABCB1), KIRC (ABCG2, CYP3A4, SLC22A2, SLC22A6), KIRP (SLC22A2), LIHC (CYP2C19, CYP2C8, CYP2C9, CYP3A5, SLC22A1), LUAD (SLC15A2), LUSC (UGT1A1), PAAD (ABCB1), SARC (ABCB1), and SKCM (ABCB1, DYPD). Overall, these data provide compelling evidence supporting ADME genes as prognostic biomarkers and potential therapeutic targets. We propose that intratumoral expression of ADME genes may impact cancer patient survival by multiple mechanisms that can include metabolizing/transporting anticancer drugs, activating anticancer drugs, and metabolizing/transporting a variety of endogenous molecules involved in metabolically fuelling cancer cells and/or controlling pro-growth signalling pathways. more...
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- 2020
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100. Establishment of Decision Rules and Risk Assessment Model for Preoperative Prediction of Lymph Node Metastasis in Gastric Cancer
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Jun Huang, Wen-jun Zhang, Chao Huang, Ce-Gui Hu, Zhengming Zhu, and Jinfeng Zhu
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0301 basic medicine ,Cancer Research ,medicine.medical_specialty ,medicine.medical_treatment ,lcsh:RC254-282 ,03 medical and health sciences ,0302 clinical medicine ,medicine ,risk factors ,Lymph node ,Original Research ,decision rules ,lymph node metastasis ,business.industry ,gastric cancer ,Decision tree learning ,Cancer ,Nomogram ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,risk assessment model ,Random forest ,preoperative diagnosis ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,Propensity score matching ,Gastrectomy ,Radiology ,Risk assessment ,business - Abstract
Background: Preoperative accurate prediction of lymph node status is especially important for the formulation of treatment plans for patients with gastric cancer (GC). The purpose of this study was to establish decision rules and a risk assessment model for lymph node metastasis (LNM) in GC using preoperative indicators. Methods: The clinical data of 554 patients who underwent gastrectomy with D2 lymphadenectomy were collected. A 1:1 propensity score matching (PSM) system was used, and the clinical data of the matched 466 patients were further analyzed. The important risk factors for LNM were extracted by the random forest algorithm, and decision rules and nomogram models for LNM were constructed with a classification tree and the “rms” package of R software, respectively. Results: Tumor size (OR: 2.058; P = 0.000), computed tomography (CT) findings (OR: 1.969; P = 0.001), grade (OR: 0.479; P = 0.000), hemoglobin (Hb) (OR: 1.211; P = 0.005), CEA (OR: 1.111; P = 0.017), and CA19-9 (OR: 1.040; P = 0.033) were independent risk factors for LNM in GC. Tumor size did rank first in the ranking of important factors for LNM in GC and was the first-level segmentation of the two initial branches of the classification tree. The accuracy, sensitivity, specificity, and positive predictive value of the decision rules in diagnosing preoperative LNM in GC were 75.6, 85.7, 73.9, 73.5, and 79.3%, respectively. The accuracy, sensitivity, and specificity of the risk assessment model in predicting preoperative LNM in GC were 79.3, 80.3, and 79.4%, respectively. Conclusion: Tumor size was the most important factor for evaluating LNM in GC. This decision rules and nomogram model constructed to take into account tumor size, CT findings, grade, hemoglobin, CEA, and CA19-9 effectively predicted the incidence of LNM in preoperative GC. more...
- Published
- 2020
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