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416 results on '"Ghioni, M."'

56. Microchips and single-photon avalanche diodes for DNA separation with high sensitivity

Author

Rech I., Cova S., Restelli A., Ghioni M., Chiari M., and Cretich M.

Abstract

Modern techniques for DNA and protein analysis and separation rely on measurements of LIF and face a trend toward employing progressively smaller samples. The currently employed detectors that provide the required ultrahigh sensitivity, e.g. photomultiplier tubes (PMTs), are bulky and/or costly and delicate, whereas a key issue for the development of compact and economical instruments is the availability of miniaturized, inexpensive, and ultrasensitive photodetectors. The planar epitaxial silicon single-photon avalanche diodes (SPADs) combine the typical advantages of microelectronics (miniaturization, ruggedness, low voltage, low power, low cost, etc.) with high sensitivity, even better than that of PMTs. The suitability of such SPADs to microchip CE has been here ascertained by developing a new apparatus with dual-wavelength LIF detection. The apparatus has been experimented in studies on the EOF suppression and on the coating stability and tested in rapid sizing of DNA fragments. The experimental results obtained in the separation of Cy5-labeled oligonucleotide demonstrate sensitivity better than 3 pM, which corresponds to less than 100 fluorescent molecules in the 50 pL illuminated volume.

Published
2006

58. Microelectronic photosensors for genetic diagnostic microsystems

Author

Rech I., Restelli A., Cova S., Ghioni M., Chiari M., and Cretich M.

Abstract

Techniques for DNA and protein analysis and separation rely on laser-induced fluorescence (LIF) and employ ultra-sensitive photo-sensors (photo-multiplier tubes or PMT's, etc.) that are bulky and costly. A key issue for compact and economical instruments is the availability of miniaturized and economical ultra-sensitive sensors. The planar silicon single-photon avalanche diodes (SPAD's) join typical microelectronic advantages (miniaturization, ruggedness, low voltage, power, and cost) to sensitivity comparable to PMT's. A compact system is described with a dual SPAD-based LIF detection system and a glass microchip for capillary electrophoresis (CE). Sensitivity better than 3 pM with injection volume 50 pl is achieved in the separation of CY5 DNA labelled fragments.

Published
2004

60. Silicon planar technology for single-photon optical detectors

Author

Sciacca E., Giudice A.C., Sanfilippo D., Zappa F., Lombardo S., Cosentino R., Di Franco C., Ghioni M., Fallica G., Bonanno G., Cova S., and Rimini E.

Subjects
Physics::Instrumentation and Detectors, Physics::Optics
Abstract

Design and fabrication of single photon avalanche detector (SPAD) in planar technology is reported. Device design and critical issues in the technology are discussed. Experimental test procedures are described for dark-counting rate, afterpulsing probability, photon timing resolution, and quantum detection efficiency. Low-noise detectors are obtained, with dark counting rates down to 10 c/s for devices with 10 mum diameter, down to 1 kc/s for 50 mum diameter. The technology is suitable for monolithic integration of SPAD detectors and associated circuits.

Published
2003

67. Cancer-testis antigen expression in triple-negative breast cancer

Author

Curigliano, G, Viale, G, Ghioni, M, Jungbluth, A, Bagnardi, V, Spagnoli, G, Neville, A, Nolè, F, Rotmensz, N, Goldhirsch, A, Jungbluth, AA, BAGNARDI, VINCENZO, Spagnoli, GC, Neville, AM, Goldhirsch, A., Curigliano, G, Viale, G, Ghioni, M, Jungbluth, A, Bagnardi, V, Spagnoli, G, Neville, A, Nolè, F, Rotmensz, N, Goldhirsch, A, Jungbluth, AA, BAGNARDI, VINCENZO, Spagnoli, GC, Neville, AM, and Goldhirsch, A.

Abstract

Background: Cancer-testis (CT) antigens, frequently expressed in human germline cells but not in somatic tissues, may become aberrantly reexpressed in different cancer types. The aim of this study was to investigate the expression of CT antigens in breast cancer. Patients and methods: A total of 100 selected invasive breast cancers, including 50 estrogen receptor (ER) positive/HER2 negative and 50 triple negative (TN), were examined for NY-ESO-1 and MAGE-A expression by immunohistochemistry. Results: A significantly higher expression of MAGE-A and NY-ESO-1 was detected in TN breast cancers compared with ER-positive tumors (P ' 0.04). MAGE-A expression was detected in 13 (26%) TN cancers compared with 5 (10%) ER-positive tumors (P ' 0.07). NY-ESO-1 expression was confirmed in nine (18%) TN tumor samples compared with two (4%) ER-positive tumors. Conclusions: MAGE-A and NY-ESO-1 CT antigens are expressed in a substantial proportion of TN breast cancers. Because of the limited therapeutic options for this group of patients, CT antigen-based vaccines might prove to be useful for patients with this phenotype of breast cancer. © The Author 2010. Published by Oxford University Press on behalf of the European Society for Medical Oncology. All rights reserved.

Published
2011

74. An extremely low-noise heralded single-photon source without temporal post-selection

Author

Piacentini, F., primary, Traina, P., additional, Della Frera, A., additional, Tosi, A., additional, Scarcella, C., additional, Ruggeri, A., additional, Gulinatti, A., additional, Ghioni, M., additional, Polyakov, S. V., additional, Migdall, A., additional, Giudice, A., additional, Brida, G., additional, Degiovanni, I. P., additional, and Genovese, M., additional

Published
2013
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76. An extremely low-noise heralded single-photon source: A breakthrough for quantum technologies

Author

Brida, G., primary, Degiovanni, I. P., additional, Genovese, M., additional, Piacentini, F., additional, Traina, P., additional, Della Frera, A., additional, Tosi, A., additional, Bahgat Shehata, A., additional, Scarcella, C., additional, Gulinatti, A., additional, Ghioni, M., additional, Polyakov, S. V., additional, Migdall, A., additional, and Giudice, A., additional

Published
2012
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88. New photon-counting detectors for single-molecule fluorescence spectroscopy and imaging

Author

Michalet, X., primary, Colyer, R. A., additional, Scalia, G., additional, Weiss, S., additional, Siegmund, O. H. W., additional, Tremsin, Anton S., additional, Vallerga, John V., additional, Villa, F., additional, Guerrieri, F., additional, Rech, I., additional, Gulinatti, A., additional, Tisa, S., additional, Zappa, F., additional, Ghioni, M., additional, and Cova, S., additional

Published
2011
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89. Photonics for Life

Author

Cubeddu, R, primary, Bassi, A, additional, Comelli, D, additional, Cova, S, additional, Farina, A, additional, Ghioni, M, additional, Rech, I, additional, Pifferi, A, additional, Spinelli, L, additional, Taroni, P, additional, Torricelli, A, additional, Tosi, A, additional, Valentini, G, additional, and Zappa, F, additional

Published
2011
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93. High-throughput single-molecule fluorescence spectroscopy using parallel detection

Author

Michalet, X., primary, Colyer, R. A., additional, Scalia, G., additional, Kim, T., additional, Levi, Moran, additional, Aharoni, Daniel, additional, Cheng, Adrian, additional, Guerrieri, F., additional, Arisaka, Katsushi, additional, Millaud, Jacques, additional, Rech, I., additional, Resnati, D., additional, Marangoni, S., additional, Gulinatti, A., additional, Ghioni, M., additional, Tisa, S., additional, Zappa, F., additional, Cova, S., additional, and Weiss, S., additional

Published
2010
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