388 results on '"Gatti, Jean-Luc"'
Search Results
52. Hosting certain facultative symbionts modulates the phenoloxidase activity and immune response of the pea aphid Acyrthosiphon pisum.
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Luo, Chen, Belghazi, Maya, Schmitz, Antonin, Lemauf, Séverine, Desneux, Nicolas, Simon, Jean‐Christophe, Poirié, Marylène, and Gatti, Jean‐Luc
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PEA aphid ,PHENOL oxidase ,IMMUNE response ,PROTEIN expression ,SURVIVAL rate ,GENE expression ,PEAS - Abstract
The pea aphid Acyrthosiphon pisum hosts different facultative symbionts (FS) which provide it with various benefits, such as tolerance to heat or protection against natural enemies (e.g., fungi, parasitoid wasps). Here, we investigated whether and how the presence of certain FS could affect phenoloxidase (PO) activity, a key component of insect innate immunity, under normal and stressed conditions. For this, we used clones of A. pisum of different genetic backgrounds (LL01, YR2 and T3‐8V1) lacking FS or harboring one or two (Regiella insecticola, Hamiltonella defensa, Serratia symbiotica + Rickettsiella viridis). Gene expression and proteomics analyses of the aphid hemolymph indicated that the two A. pisum POs, PPO1 and PPO2, are expressed and translated into proteins. The level of PPO genes expression as well as the amount of PPO proteins and phenoloxidase activity in the hemolymph depended on both the aphid genotype and FS species. In particular, H. defensa and R. insecticola, but not S. symbiotica + R. viridis, caused a sharp decrease in PO activity by interfering with both transcription and translation. The microinjection of different types of stressors (yeast, Escherichia coli, latex beads) in the YR2 lines hosting different symbionts affected the survival rate of aphids and, in most cases, also decreased the expression of PPO genes after 24 h. The amount and activity of PPO proteins varied according to the type of FS and stressor, without clear corresponding changes in gene expression. These data demonstrate that the presence of certain FS influences an important component of pea aphid immunity. [ABSTRACT FROM AUTHOR]
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- 2021
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53. The motile beta/IC1 subunit of sea urchin sperm outer arm dynein does not form a rigor bond
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Moss, Anthony G., Gatti, Jean-Luc, and Witman, George B.
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Sea urchins -- Physiological aspects ,Spermatozoa -- Motility ,Dynein -- Research ,Biological sciences - Abstract
The microtubule-binding properties of the outer arm dynein of sea urchin sperm and its beta/IC1 subunit were studied by in vitro translocation and cosedimentation assays. The results showed that isolated sea urchin outer arm dynein and purified beta/IC1 subunit adsorbed to a glass coverslip can bind and translocate microtubules in the presence of sufficient ATP. Insufficient ATP causes microtubule release, which contradicts previous reports of rigor cross-bridges to microtubules under this condition. It appears that intact dynein has subunits that cause the observed rigor cross-bridges under insufficient ATP.
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- 1992
54. Chapter 9 Isolation of Fish Sperm Flagella
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Saudrais, Christèle, primary and Gatti, Jean-Luc, additional
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- 1995
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55. Les conquêtes de l’INRA pour le biocontrôle
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Joly, Emmanuelle, Frerot, Brigitte, Marion-Poll, Frédéric, Thiery, Denis, Martin, Jean-Claude, Levevre, Amélie, Navajas , Maria, Tabone, Elisabeth, Rasplus, Jean-Yves, Ris, Nicolas, Ogier, Jean-Claude, Pagès, Sylvie, Givaudan, Alain, Gaudriault, Sophie, Gatti, Jean-Luc, Lavigne, Claire, Bouvier, Jean-Charles, Mailleret, Ludovic, Vercken, Elodie, Klein, Etienne, Roques, Lionel, Desneux, Nicolas, Poncet, Christine, Bardin, Marc, Nicot, Philippe, Ponchet, Michel, Galiana, Eric, Brisset, Marie-Noëlle, and Malausa, Thibaut
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macroorganisme ,Biocontrôle ,micro-organismes ,Contrôle des ravageurs ,lutte contre les ravageurs - Abstract
Lancé à l’occasion du Grenelle de l’environnement de 2007, le plan Ecophyto a pour objectif de réduire de moitié le recours aux produits phytopharmaceutiques en France, à l’horizon 2025. Certaines des substances jugées préoccupantes ont déjà été retirées du marché et d’autres, à l’instar du glyphosate ou des néonicotinoïdes, le seront dans les années à venir. C’est une excellente nouvelle pour l’environnement comme pour notre santé. Mais ça n’est pas sans conséquence pour l’ensemble des filières agricoles. En effet, par quoi va-t-on remplacer les produits phytosanitaires pour protéger les cultures des bioagresseurs qui les menacent? Ne risque-t-on pas de voir ressurgir des maladies ou des insectes phytophages jusqu’alors efficacement contrôlés? Et comment lutter contre les nouvelles espèces envahissantes venues du bout du monde et qui se répandent à toute vitesse sur notre territoire? Pour faire face à ces immenses défis, l’Inra est en première ligne. Et parmi les stratégies mises en œuvre pour y répondre, le biocontrôle figure en tête de liste. Le biocontrôle, c’est un ensemble de méthodes de protection des cultures basées sur l’utilisation d’organismes vivants ou de substances naturelles. Les produits de biocontrôle sont classés en quatre catégories: les macro-organismes (principalement insectes, nématodes, acariens...), les micro-organismes (virus, bactéries, champignons...), les médiateurs chimiques tels que les phéromones et enfin les substances naturelles d’origine minérale, végétale ou animale. L’Inra n’a pas attendu Ecophyto pour s’intéresser au biocontrôle. Depuis plusieurs décennies, les chercheurs mènent des travaux sur les interactions entre les plantes et leurs ravageurs. Ils développent des méthodes de lutte qui, utilisées seules ou dans le cadre d’une stratégie de contrôle globale, jouent un rôle croissant dans la protection des cultures. Et l’Institut est à l’origine d’innovations qui sont utilisées dans le monde entier, telles que la confusion sexuelle à l’aide de phéromones. En tant qu’organisme public de recherche finalisée, l’Inra s’associe à des partenaires privés pour mettre au point des solutions de biocontrôle et étudie leurs impacts sur la santé et l’environnement. L’Institut propose également des pistes pour aménager les paysages agricoles, en vue de favoriser le développement ou la conservation des auxiliaires de cultures, alliés précieux en agriculture.
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- 2018
56. Functional insights from the GC-poor genomes of two aphid parasitoids,Aphidius erviandLysiphlebus fabarum
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Dennis, Alice B., primary, Ballesteros, Gabriel I., additional, Robin, Stéphanie, additional, Schrader, Lukas, additional, Bast, Jens, additional, Berghöfer, Jan, additional, Beukeboom, Leo, additional, Belghazi, Maya, additional, Bretaudeau, Anthony, additional, Büllesbach, Jan, additional, Cash, Elizabeth, additional, Colinet, Dominique, additional, Dumas, Zoé, additional, Falabella, Patrizia, additional, Gatti, Jean-Luc, additional, Geuverink, Elzemiek, additional, Gibson, Joshua D., additional, Hertäg, Corinne, additional, Hartmann, Stefanie, additional, Jacquin-Joly, Emmanuelle, additional, Lammers, Mark, additional, Lavandero, Blas I., additional, Lindenbaum, Ina, additional, Massardier-Galata, Lauriane, additional, Meslin, Camille, additional, Montagné, Nicolas, additional, Pak, Nina, additional, Poirié, Marylène, additional, Salvia, Rosanna, additional, Smith, Chris R., additional, Tagu, Denis, additional, Tares, Sophie, additional, Vogel, Heiko, additional, Schwander, Tanja, additional, Simon, Jean-Christophe, additional, Figueroa, Christian C., additional, Vorburger, Christoph, additional, Legeai, Fabrice, additional, and Gadau, Jürgen, additional
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- 2019
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57. Rapid and differential evolution of the venom composition of a parasitoid wasp depending on the host strain
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Cavigliasso, Fanny, primary, Mathé-Hubert, Hugo, additional, Kremmer, Laurent, additional, Rebuf, Christian, additional, Gatti, Jean-Luc, additional, Malausa, Thibaut, additional, Colinet, Dominique, additional, and Poirié, Marylène, additional
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- 2019
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58. Venom Atypical Extracellular Vesicles as Interspecies Vehicles of Virulence Factors Involved in Host Specificity: The Case of a Drosophila Parasitoid Wasp
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Wan, Bin, primary, Goguet, Emilie, additional, Ravallec, Marc, additional, Pierre, Olivier, additional, Lemauf, Séverine, additional, Volkoff, Anne-Nathalie, additional, Gatti, Jean-Luc, additional, and Poirié, Marylène, additional
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- 2019
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59. The preference–performance relationship as a means of classifying parasitoids according to their specialization degree
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Monticelli, Lucie S., primary, Nguyen, Le Thu Ha, additional, Amiens‐Desneux, Edwige, additional, Luo, Chen, additional, Lavoir, Anne‐violette, additional, Gatti, Jean‐Luc, additional, and Desneux, Nicolas, additional
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- 2019
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60. Drosophila Cellular Immunity Against Parasitoid Wasps: A Complex and Time-Dependent Process
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Kim-Jo, Chami, primary, Gatti, Jean-Luc, additional, and Poirié, Marylène, additional
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- 2019
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61. Differential side-effects ofBacillus thuringiensisbioinsecticide on non-targetDrosophilaflies
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Babin, Aurélie, primary, Nawrot-Esposito, Marie-Paule, additional, Gallet, Armel, additional, Gatti, Jean-Luc, additional, and Poirié, Marylène, additional
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- 2019
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62. Expression, immunolocalization and processing of fertilins ADAM-1 and ADAM-2 in the boar (sus domesticus) spermatozoa during epididymal maturation
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Bonet Sergi, Puigmulé Marta, Gatti Jean-Luc, Dacheux Jean-Louis, Guyonnet Benoît, Fàbrega Anna, and Pinart Elisabeth
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Gynecology and obstetrics ,RG1-991 ,Reproduction ,QH471-489 - Abstract
Abstract Fertilin alpha (ADAM-1) and beta (ADAM-2) are integral membrane proteins of the ADAM family that form a fertilin complex involved in key steps of the sperm-oocyte membrane interaction. In the present work, we analyzed the presence of ADAM-1 and ADAM-2 mRNAs, the spermatozoa proteins' processing and their sub-cellular localization in epididymal samples from adult boars. ADAM-1 and ADAM-2 mRNAs were highly produced in the testis, but also in the vas efferens and the epididymis. On immunoblots of sperm extracts, ADAM-1 subunit appeared as a main reactive band of ~50-55 kDa corresponding to occurrence of different isoforms throughout the epididymal duct, especially in the corpus region where isoforms ranged from acidic to basic pI. In contrast, ADAM-2 was detected as several bands of ~90 kDa, ~75 kDa, ~50-55 kDa and ~40 kDa. The intensity of high molecular mass bands decreased progressively in the distal corpus where lower bands were also transiently observed, and only the ~40 kDa was observed in the cauda. The presence of bands of different molecular weights likely results from a proteolytic processing occurring mainly in the testis for ADAM-1, and also throughout the caput epididymis for ADAM-2. Immunolocalization showed that fertilin migrates from the acrosomal region to the acrosomal ridge during the sperm transit from the distal corpus to the proximal cauda. This migration is accompanied by an important change in the extractability of a part of ADAM-1 from the sperm membrane. This suggests that the fertilin surface migration may be triggered by the biochemical changes induced by the epididymal post-translational processing of both ADAM1 and ADAM-2. Different patterns of fertilin immunolocalization then define several populations of spermatozoa in the cauda epididymis. Characterization of such fertilin complex maturation patterns is an important step to develop fertility markers based on epididymal maturation of surface membrane proteins in domestic mammals.
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- 2011
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63. The adult boar testicular and epididymal transcriptomes
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Guyonnet Benoît, Marot Guillemette, Dacheux Jean-Louis, Mercat Marie-José, Schwob Sandrine, Jaffrézic Florence, and Gatti Jean-Luc
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Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Mammalians gamete production takes place in the testis but when they exit this organ, although spermatozoa have acquired a specialized and distinct morphology, they are immotile and infertile. It is only after their travel in the epididymis that sperm gain their motility and fertility. Epididymis is a crescent shaped organ adjacent to the testis that can be divided in three gross morphological regions, head (caput), body (corpus) and tail (cauda). It contains a long and unique convoluted tubule connected to the testis via the efferent ducts and finished by joining the vas deferens in its caudal part. Results In this study, the testis, the efferent ducts (vas efferens, VE), nine distinct successive epididymal segments and the deferent duct (vas deferens, VD) of four adult boars of known fertility were isolated and their mRNA extracted. The gene expression of each of these samples was analyzed using a pig generic 9 K nylon microarray (AGENAE program; GEO accession number: GPL3729) spotted with 8931 clones derived from normalized cDNA banks from different pig tissues including testis and epididymis. Differentially expressed transcripts were obtained with moderated t-tests and F-tests and two data clustering algorithms based either on partitioning around medoid (top down PAM) or hierarchical clustering (bottom up HCL) were combined for class discovery and gene expression analysis. Tissue clustering defined seven transcriptomic units: testis, vas efferens and five epididymal transcriptomic units. Meanwhile transcripts formed only four clusters related to the tissues. We have then used a specific statistical method to sort out genes specifically over-expressed (markers) in testis, VE or in each of the five transcriptomic units of the epididymis (including VD). The specific regional expression of some of these genes was further validated by PCR and Q-PCR. We also searched for specific pathways and functions using available gene ontology information. Conclusion This study described for the first time the complete transcriptomes of the testis, the epididymis, the vas efferens and the vas deferens on the same species. It described new genes or genes not yet reported over-expressed in these boar tissues, as well as new control mechanisms. It emphasizes and fulfilled the gap between studies done in rodents and human, and provides tools that will be useful for further studies on the biochemical processes responsible for the formation and maintain of the epididymal regionalization and the development of a fertile spermatozoa.
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- 2009
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64. [18] Purification and characterization of Salmo gairdneri outer arm dynein
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Moss, Anthony G., primary, Gatti, Jean-Luc, additional, King, Stephen M., additional, and Witman, George B., additional
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- 1991
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65. Internalisation of Leptopilina boulardi venosomes in Drosophila melanogaster lamellocytes : specificity and mechanism
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Wan, Bin, Poirie, Marylène, Gatti, Jean-Luc, Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), 'Investments for the Future' LABEX SIGNALIFE: program reference ANR-11-LABX-0028, Institut National des Sciences Appliquées de Lyon (INSA Lyon). FRA., Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), and Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)
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Leptopilina boulardi venosomes ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,Venom of the ISm strain of L. boulardi ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,Internalisation ,[SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity ,Drosophila melanogaster lamellocytes - Abstract
Leptopilina boulardi wasps are parasitoids that develop inside Drosophila melanogaster larvae by consuming their tissues, leading to the host death. They rely on venom injection at oviposition to circumvent the host immune response and ensure parasitism success. Venom of the ISm strain of L. boulardi mainly targets the main hemocyte type involved in the cellular encapsulation of parasitoid eggs, the lamellocytes, and induce changes in their morphology. We previously demonstrated that venosomes, a specific type of extra-cellular vesicles (EVs) found in LbISm venom are required for parasitism success. Recent data also confirmed that they transport several venom-specific RhoGTPase-activating proteins (e.g. LbGAP and LbGAP2) into the host lamellocytes. However, the host specificity and the mechanism of venosomes entry inside lamellocytes yet remained to be elucidated. Here, we show that venosomes are internalized by lamellocytes at a high level in D. melanogaster (wild type and Hoptuml mutant that constitutively produces numerous lamellocytes) and at a low level in D. yakuba. Whereas they are not found at all in D. suzukii lamellocyte-like cells, suggesting a species-specific mechanism of entry. In D. melanogaster lamellocytes, LbGAP colocalize with endosomal Rab7 protein, suggesting a receptor-mediated endocytosis of venosomes. To characterize the lamellocyte membrane receptor of venosomes, we have set up methods to separate Hoptuml lamellocytes from other hemocytes and purify their membranes. Preliminary results will be presented.
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- 2017
66. Non-intentional impacts of the bioinsecticide Bacillus thuringiensis on non-target insect species
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Babin, Aurélie, Esposito, Marie-Paule, Gallet, Armel, Gatti, Jean-Luc, Poirie, Marylène, Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), ANR ImBio, Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA)
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[SDV]Life Sciences [q-bio] ,fungi ,bioinsecticide ,Bacillus thuringiensis - Abstract
Environmental and health issues resulting from the intensive use of broad-spectrum chemical insecticides, have favored the spread of biological insecticides in the last decades. The most common, Bacillus thuringiensis (Bt), is a spore-forming bacterium which produces a varied range of insecticidal toxins. However, the field accumulation of spores and toxins of Bt kurstaki (Btk), the Bt subspecies most commonly used against lepidopteran pests, potentially increases the risk for non-target biodiversity. Using mainly the Lepidoptera-targeting Bt subspecies kurstaki (Btk) and the well-studied laboratory models Drosophila spp. as non-target insects, we tested the potential non-intentional effects of the ingestion of commercial Btk formulations on traits related to the fly development and the adult fitness. We observed a delayed and reduced emergence of Drosophila melanogaster flies at doses above 107 CFU/g of food (close to the doses used for fields treatments), regardless of the fly strain, the commercial formulation, and the rearing medium. Mortality affected mainly the early larval stage while all the larvae died before pupation at the highest dose (109 CFU/g). We analyzed the respective roles of the formulation components (spores, toxins, additives…), and found that the developmental and lethal effects may result from a synergy between Btk spores and toxins. Developmental and lethal impacts were also observed for other Drosophila species, with variation in the LD50 and in the effect amplitude. Our work suggests that the environmental accumulation of Bt may have possible non-intentional effects on non-target Drosophila species and the associated species communities.
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- 2017
67. Deciphering Drosophila immune resistance to endoparasitoid wasps: mechanisms, specificity and evolution
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Kim, Chami, Gatti, Jean-Luc, Poirie, Marylène, Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), 'Investments for the Future' LABEX SIGNALIFE: program reference ANR-11-LABX-0028, Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), and Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)
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mechanisms ,animal structures ,[SDV]Life Sciences [q-bio] ,fungi ,Deciphering Drosophila ,immune resistance ,endoparasitoid wasps - Abstract
Drosophila melanogaster is a model organism for many fields in biology including immunology.Drosophila innate immunity is well described in case of bacterial and fungal infections but otherparasites, such as parasitoids, can be harmful and even lethal to Drosophila.We are working on the immune interaction between Drosophila larvae and the endoparasitic wasp,Leptopilina boulardi, a species that lay eggs inside Drosophila larvae. The parasitoid larval stages thendevelop at the expense of the host leading to its death. The adult parasitoid emerges from theDrosophila pupa.Drosophila larvae recognizes the parasitoid egg as a foreign body and, depending on the fly strain, theycan mount a successful immune response: the egg is surrounded by layers of hemocytes forming amelanized capsule, and it is a fly that will emerge. To counteract the immune response, female waspsrely on venom components injected together with the egg which prevent the encapsulation.A major biallelic gene (alleles R and S) responsible for the resistance / susceptibility of Drosophila to L.boulardi has been identified. I am involved in deciphering the role of this gene in the resistancemechanism using different approaches including: Flow cytometry to track the variation in quantity /proportion of the different types of hemocytes following parasitism; Bioinformatics to identifypotential interactants of this gene; Mutants and strains UAS-RNAi to understand the potential role ofthese genes in the anti-parasitic immune defence of Drosophila.
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- 2017
68. Aphid infestation differently affects the defences of nitrate-fed and nitrogenfixing Medicago truncatula and alters symbiotic nitrogen fixation.
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Pandharikar, Gaurav, Gatti, Jean-Luc, Simon, Jean-Christophe, Frendo, Pierre, and Poirié, Marylène
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NITROGEN fixation , *MEDICAGO truncatula , *MEDICAGO , *PEA aphid , *APHIDS , *ROOT-tubercles , *PROTEIN expression - Abstract
Legumes can meet their nitrogen requirements through root nodule symbiosis, which could also trigger plant systemic resistance against pests. The pea aphid Acyrthosiphon pisum, a legume pest, can harbour different facultative symbionts (FS) influencing various traits of their hosts. It is therefore worth determining if and how the symbionts of the plant and the aphid modulate their interaction. We used different pea aphid lines without FS or with a single one (Hamiltonella defensa, Regiella insecticola, Serratia symbiotica) to infest Medicago truncatula plants inoculated with Sinorhizobium meliloti (symbiotic nitrogen fixation, SNF) or supplemented with nitrate (non-inoculated, NI). The growth of SNF and NI plants was reduced by aphid infestation, while aphid weight (but not survival) was lowered on SNF compared to NI plants. Aphids strongly affected the plant nitrogen fixation depending on their symbiotic status, suggesting indirect relationships between aphid- and plant-associated microbes. Finally, all aphid lines triggered expression of Pathogenesis-Related Protein 1 (PR1) and Proteinase Inhibitor (PI), respective markers for salicylic and jasmonic pathways, in SNF plants, compared to only PR1 in NI plants. We demonstrate that the plant symbiotic status influences plant-aphid interactions while that of the aphid can modulate the amplitude of the plant's defence response. [ABSTRACT FROM AUTHOR]
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- 2020
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69. Time-course analysis of Drosophila suzukii interaction with endoparasitoid wasps evidences a delayed encapsulation response compared to D. melanogaster
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Iacovone, Alessia, primary, Ris, Nicolas, additional, Poirié, Marylène, additional, and Gatti, Jean-Luc, additional
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- 2018
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70. Commercial Bacillus thuringiensis formulations have non-intentional adverse effects on Drosophila species
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Babin, Aurélie, Esposito, Marie-Paule, Gallet, Armel, Gatti, Jean-Luc, Poirie, Marylène, Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), ANR ImBio, Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA)
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Drosophila species ,animal structures ,[SDV]Life Sciences [q-bio] ,fungi ,Commercial Bacillus thuringiensis formulations ,non-intentional adverse effects - Abstract
Commercial Bacillus thuringiensis formulations have non-intentional adverse effects on Drosophila species. German Drosophila Meeting
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- 2016
71. Field Monitoring of Drosophila suzukii and Associated Communities in South Eastern France as a Pre-Requisite for Classical Biological Control
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Kremmer, Laurent, primary, Thaon, Marcel, additional, Borowiec, Nicolas, additional, David, Jean, additional, Poirié, Marylène, additional, Gatti, Jean-Luc, additional, and Ris, Nicolas, additional
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- 2017
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72. Biochemical characterization and comparison of aspartylglucosaminidases secreted in venom of the parasitoid wasps Asobara tabida and Leptopilina heterotoma
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Coulette, Quentin, primary, Lemauf, Séverine, additional, Colinet, Dominique, additional, Prévost, Geneviève, additional, Anselme, Caroline, additional, Poirié, Marylène, additional, and Gatti, Jean-Luc, additional
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- 2017
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73. Ram seminal plasma proteome and impact on sperm liquid preservation
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Soleilhavoup, Clément, Tsikis, Guillaume, Labas, Valérie, Harichaux, Grégoire, Kohnke, Philippa, Dacheux, Jean-Louis, Guérin, Yvon, Gatti, Jean-Luc, de Graaf, S.P., Druart, Xavier, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Plate-forme d'Analyse Intégrative des Biomolécules, Institut National de la Recherche Agronomique (INRA), Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Faculty of Veterinary Science, The University of Sydney, Université de Haute Bretagne ( Rennes 2 ) (UR 2). Rennes, FRA., and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
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[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] ,spectral counting ,spermatozoa ,preservation ,proteome ,zinc ,seminal plasma ,alpha glycoprotein ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2015
74. Statistical analysis of the individual variability of 1D protein profiles as a tool in ecology
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Mathe-Hubert, Hugo, Cavigliasso, Fanny, Gatti, Jean-Luc, Colinet, Dominique, Malausa, Thibaut, Poirie, Marylène, Université de Nice Sophia-Antipolis (UNSA), Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), and Université Claude Bernard Lyon 1 (UCBL). FRA.
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[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2015
75. Parasitoid wasp venom vesicles (venosomes) enter Drosophila melanogasterlamellocytes through a flotillin/lipid raft-dependent endocytic pathway
- Author
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Wan, Bin, Poirié, Marylène, and Gatti, Jean-Luc
- Abstract
ABSTRACTVenosomes are extracellular vesicles found in the venom of Leptopilinaendoparasitoids wasps, which transport and target virulence factors to impair the parasitoid egg encapsulation by the lamellocytes of their Drosophila melanogasterhost larva. Using the co-immunolocalization of fluorescent L. boulardivenosomes and one of the putative-transported virulence factors, LbGAP, with known markers of cellular endocytosis, we show that venosomes endocytosis by lamellocytes is not a process dependent on clathrin or macropinocytosis and internalization seems to bypass the early endosomal compartment Rab5. After internalization, LbGAP colocalizes strongly with flotillin-1 and the GPI-anchored protein Atilla/L1 (a lamellocyte surface marker) suggesting that entry occurs viaa flotillin/lipid raft-dependent pathway. Once internalized, venosomes reach all intracellular compartments, including late and recycling endosomes, lysosomes, and the endoplasmic reticulum network. Venosomes therefore enter their target cells by a specific mechanism and the virulence factors are widely distributed in the lamellocytes’ compartments to impair their functions.
- Published
- 2020
- Full Text
- View/download PDF
76. The spotted wing drosophila: interactions between Drosophila suzukii and native versus local parasitoid species
- Author
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Iacovone, Alessia, Ris, Nicolas, Poirié, Marylène, and Gatti, Jean-Luc
- Subjects
drosophila suzukii ,entomophage - Published
- 2014
77. Comparative venomics of Psyttalia lounsburyi and P. concolor, two olive fruit fly parasitoids: a hypothetical role for a GH1 β-glucosidase
- Author
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Mathé-Hubert, Hugo, primary, Colinet, Dominique, additional, Deleury, Emeline, additional, Belghazi, Maya, additional, Ravallec, Marc, additional, Poulain, Julie, additional, Dossat, Carole, additional, Poirié, Marylène, additional, and Gatti, Jean-Luc, additional
- Published
- 2016
- Full Text
- View/download PDF
78. Variability and evolution of the venom of two biological control agents from the genus Psyttalia
- Author
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Colinet, Dominique, Belghazi, Maya, Thaon, Marcel, Gatti, Jean-Luc, Ris, Nicolas, Malausa, Thibaut, Poirie, Marylène, and Mathe-Hubert, Hugo
- Subjects
Animal biology ,protéines du venin ,parasitoïde ,protéine wasp ,condition contrôlée ,Microbiology and Parasitology ,trait phénotypique ,venin ,approche protéomique ,Microbiologie et Parasitologie ,étude transcriptomique ,contrôle de la qualité ,Biologie animale ,variation intrapopulation ,guêpe ,biologie de l'insecte ,évolution ,électrophorèse ,contrôle biologique ,bactrocera oleae - Published
- 2013
79. Chapter 15 - Diversity of Virus-Like Particles in Parasitoids’ Venom: Viral or Cellular Origin?
- Author
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Gatti, Jean-luc, Schmitz, Antonin, Colinet, Dominique, and Poirié, Marylène
- Published
- 2012
- Full Text
- View/download PDF
80. Rôle des gamma-glutamyl transpeptidases dans les relations hôte/parasitoïde Acyrthosiphon pisum/Aphidius ervi
- Author
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Tares-Amichot, Sophie, Anselme, Caroline, Arthaud Hugonin, Laury, Gatti, Jean-Luc, Poirie, Marylène, Colinet, Dominique, Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), and Université de Picardie Jules Verne (UPJV)
- Subjects
[SDV]Life Sciences [q-bio] ,[SDE]Environmental Sciences ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2012
81. Closely related parasites targeting the same host can show extensive venom variation: the case of Leptopilina parasitoids of Drosophila
- Author
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Colinet, Dominique, Deleury, Emeline, Anselme, Caroline, Cazes, Dominique, Poulain, Julie, Belghazi, Maya, Gatti, Jean-Luc, Poirie, Marylène, Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
interspecific ,intraspecific ,[SDV]Life Sciences [q-bio] ,[SDE]Environmental Sciences ,venom ,variation ,parasitoid ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2012
82. Closely related parasites targeting the same host can show extensive venom variation: the case of Leptopilina parasitoids of Drosophila
- Author
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Anselme, Caroline, Cazes, Dominique, Poulain, Julie, Belghazi, Maya, Gatti, Jean-Luc, Poirié, Marylène, Colinet, Dominique, and Deleury, Emeline
- Subjects
parasitoïde ,leptopilina ,venin ,interspecific ,intraspecific ,variation ,venom ,parasitoid ,drosophila - Published
- 2012
83. Effets de l'élevage sur un hôte de substitution chez les parasitoïdes Psyttalia lounsburyi et P. concolor utilisés en lutte biologique : Une approche de vénomique
- Author
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Colinet, Dominique, Belghazi, Maya, Thaon, Marcel, Gatti, Jean-Luc, Ris, Nicolas, Malausa, Thibaut, Poirie, Marylène, and Mathe-Hubert, Hugo
- Subjects
Animal biology ,endoparasitoïde ,Parasitologie ,ceratitis capitata ,outil moléculaire ,trait phénotypique ,relation hôte-parasite ,venin ,lutte biologique ,hôte de substitution ,élevage ,facteur de virulence ,lâcher ,stratégie de reproduction ,physiologie de l'hôte ,diversité génétique ,Biologie animale ,impact ,sélection ,Parasitology ,hôte naturel ,ponte - Abstract
Les endoparasitoïdes pondent à l ’ intérieur d 'un insecte hôte dans lequel ils se développent, entraînant sa mort. Leur succès reproducteur dépend donc de leur capacité à contrôler l'immunité et la physiologie de l'hôte (Dupas et al. 2003). Les stratégies utilisées font notamment appel à l'injection de facteurs de virulence lors de la ponte, la plupart étant des protéines contenues dans le venin (Pennachio et Strand, 2006) qui peuvent être étudiées par des approches dites de "vénomique". Les parasitoïdes sont souvent utilisés en lutte biologique, mais leur qualité au moment des lâchés sur le terrain, lorsqu'elle est évaluée, est généralement étudiée via des mesures de traits phénotypiques peu pertinents et des outils moléculaires d'estimation de la diversité génétique, sans lien avec les traits précédents. Par exemple, leur production s'effectue souvent sur un hôte de substitution. L’ impact de ce changement d'hôte n'est pas ou peu connu. Il pourrait être une force sélective sur les stratégies de virulence de l’auxiliaire. Il est donc susceptible d’induire une maladaptation à l’hôte naturel, c'est-à- dire la cible finale de la lutte biologique. Les démonstrations expérimentales de tels phénomènes sont très rares. L'étude est menée pour 2 espèces de parasitoïde, Psyttalia lounsburyi et concolor , utilisés pour lutter contre la mouche de l ’ olive Bactrocera oleae . Les souches parasitoïdes sont élevées au laboratoire sur l'hôte de substitution Ceratitis capitata (mouche des fruits) que seul P. concolor est naturellement capable de parasiter. L'étude consiste à caractériser des protéines du venin et leur variation inter-espèce, inter-souche et inter-individu et ensuite à tester leur évolution en réponse au changement d’hôte. Actuellement, 5 souches sont maintenues au laboratoire sur C. capitata : une souche de P. concolor depuis 13 générations, 2 souches de P. lounsburyi depuis environ 100 générations (une Sud Africaine, une Kényane, initialement fortement différenciées (F ST = 0,4). Par ailleurs, les populations de P. lounsburyi ont très récemment été ré-échantillonnée sur les sites précédemment échantillonnés il y a 7 ans. Ces souches permettront d'étudier l'évolution du venin et du succès parasitaire dans les conditions d'élevage, suite au changement d'hôte.
- Published
- 2012
84. Virus-like particles in parasitoids' venom: viral or cellular origin?
- Author
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Goguet-Surmenian, Emilie, Belghazi, Maya, Ravallec, Marc, Rebuf, Christian, Colinet, Dominique, Poirie, Marylene, Gatti, Jean-Luc, Interactions Biotiques et Santé Végétale, Institut National de la Recherche Agronomique (INRA), Université de la Méditerranée - Aix-Marseille 2, Diversité, Génomes et Interactions Microorganismes-Insectes, and Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)
- Subjects
BIOCHIMIE ,[SDV]Life Sciences [q-bio] ,VIRUS PARTICLE LIKE ,PARASITOID ,IMMUNITY ,TOXICITY ,DROSOPHILA ,VENOSOME ,LEPTOPILINA BOULARDI ,DIPTERA ,BIOCHEMISTRY ,PARASITISM ,ComputingMilieux_MISCELLANEOUS ,RELATION HOTE-PARASITE - Abstract
National audience
- Published
- 2011
85. Virus-like particles in parasitoids' venom: viral or cellular origin ?: Virus-like particles, venom, wasps, Leptopilina, Drosophila
- Author
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Gatti, Jean-Luc, Surmenian, Emilie, Schmitz, Antonin, Belghazi, Maya, Colinet, Dominique, Poirie, Marylène, Interactions Biotiques et Santé Végétale, Institut National de la Recherche Agronomique (INRA), and Université de la Méditerranée - Aix-Marseille 2
- Subjects
[SDV]Life Sciences [q-bio] - Published
- 2011
86. How parasitoid wasps vary in virulence: what venom tell us
- Author
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Colinet, Dominique, Anselme, Caroline, Deleury, Emeline, Belghazi, Maya, Poulain, J., Gatti, Jean-Luc, Poirie, Marylene, Interactions Biotiques et Santé Végétale, Institut National de la Recherche Agronomique (INRA), and Université de Nice Sophia-Antipolis (UNSA)
- Subjects
LEPTOPILINA HETEROTOMA ,APHIDIUS ERVI ,[SDV]Life Sciences [q-bio] ,HYMENOPTERA ,FIGITIDAE ,lutte biologique ,DROSOPHILA ,LEPTOPILINA BOULARDI ,APHIDIINAE ,VENOM ,VIRULENCE FACTORS ,VARIABILITY ,PROTEOMICS ,SELECTIVE PARAMETERS ,RELATION HOTE-PARASITE ,température ,toxicité - Published
- 2011
87. Diversity of virus-like particles in parasitoids’ venom: viral or cellular origin?
- Author
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Gatti, Jean-Luc, Schmitz, Antonin, Colinet, Dominique, Poirie, Marylène, Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Nancy Beckage, and Jean-Michel Drezen
- Subjects
figitidae ,vesicles ,[SDV]Life Sciences [q-bio] ,fungi ,[SDE]Environmental Sciences ,braconidea ,venom ,exosomes ,ovaries ,host immune suppression - Abstract
Chapitre 15; International audience; This chapter reviews data available on parasitoid wasps' VLPs, mainly on the basis of their structure and composition. This leads to the discussion how to discriminate between virus-resembling VLPs and viruses, and to suggest that some of the VLPs might be cellular secreted vesicles, such as microvesicles and exosomes described in many epithelium and cells, aimed to transfer toxins inside host cells. Finally, it proposes to avoid the misleading name of VLPs for vesicles produced in venom and rather use the term of “venosomes.” During oviposition in their insect hosts, most endoparasitic wasps inject venom and/or ovarian products that will either prevent the egg from being recognized by the host immune system or protect it from the immune response. This response, the encapsulation process, involves both hematopoietic cells that form successive layers around the parasitoid egg, and induction of the humoral melanization process, due to activation of the phenoloxidase cascade. Among components injected by the wasp with its eggs, several immune suppressive proteins have been described that suppress either the cellular or the humoral defenses. In species from specific braconid and ichneumonid groups, viral capsids containing multiple DNA circles (polydnaviruses, PDVs) produced in the ovary fluid are injected into the host and enter host cells.
- Published
- 2011
- Full Text
- View/download PDF
88. Variation intra et interspécifique du venin chez un auxiliaire de lutte biologique
- Author
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Colinet, Dominique, Belghazi, Maya, Thaon, Marcel, Gatti, Jean-Luc, Ris, Nicolas, Poirié, Marylène, and Mathé-Hubert, Hugo
- Subjects
protéine ,lutte biologique ,PSYTTALIA CONCOLOR ,PSYTTALIA LOUNSBURYI ,BACTROCERA OLEAE ,CERATITIS CAPITATA ,VENOM ,SUBSTITUTE HOST ,VARIABILITY ,PROTEOMICS ,BIOLOGICAL CONTROL ,RELATION HOTE-PARASITE - Published
- 2011
89. Epididymal proteome, transcriptome, what else
- Author
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Gatti, Jean-Luc, Guyonnet, Benoit, Belleannée, Clémence, Dacheux, Françoise, Dacheux, Jean-Louis, Interactions Biotiques et Santé Végétale, Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
épididyme ,BIOCHIMIE ,maturation ,[SDV]Life Sciences [q-bio] ,reproduction animale ,fertilité animale ,EPIDIDYMIS ,PROTEIN COMPOSITION ,ELECTROPHORESIS ,SPERM PRESERVATION ,BIOCHEMISTRY ,PROTEOMIC ANALLYSIS ,propriété biochimique ,membrane cellulaire ,protéine animale ,protéine ,[INFO]Computer Science [cs] ,protéome ,électrophorèse ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2010
90. Rôle de la composition protéique du plasma séminal dans la résistance osmotique des spermatozoïdes
- Author
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Druart, Xavier, Guillouet, Philippe, Ferchaud, Stéphane, Reynaud, Karine, Theau Clément, Michèle, Labas, Valérie, Gatti, Jean-Luc, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Insémination Caprine et Porcine (ICP), Institut National de la Recherche Agronomique (INRA), Biologie du développement et reproduction (BDR), Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,RESISTANCE OSMOTIQUE ,[INFO] Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS ,SPERMATOZOIDE ,PLASAMA SEMINAL - Abstract
National audience
- Published
- 2010
91. Transgenic Rabbits Expressing Ovine PrP Are Susceptible to Scrapie
- Author
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Sarradin, Pierre, primary, Viglietta, Céline, additional, Limouzin, Claude, additional, Andréoletti, Olivier, additional, Daniel-Carlier, Nathalie, additional, Barc, Céline, additional, Leroux-Coyau, Mathieu, additional, Berthon, Patricia, additional, Chapuis, Jérôme, additional, Rossignol, Christelle, additional, Gatti, Jean-Luc, additional, Belghazi, Maya, additional, Labas, Valérie, additional, Vilotte, Jean-Luc, additional, Béringue, Vincent, additional, Lantier, Frédéric, additional, Laude, Hubert, additional, and Houdebine, Louis-Marie, additional
- Published
- 2015
- Full Text
- View/download PDF
92. In vivo imaging of in situ motility of fresh and liquid-stored ram spermatozoa in the ewe genital tract
- Author
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Druart, Xavier, Cognie, Juliette, Baril, Gérard, Clement, Frédéric, Dacheux, Jean-Louis, Gatti, Jean-Luc, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), SIgnals and SYstems in PHysiology & Engineering (SISYPHE), Inria Paris-Rocquencourt, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration
- Subjects
[SDV.SA.SPA]Life Sciences [q-bio]/Agricultural sciences/Animal production studies ,IMAGERIE ,[SDV.SA.SPA] Life Sciences [q-bio]/Agricultural sciences/Animal production studies ,ComputingMilieux_MISCELLANEOUS - Abstract
Communication orale 535; International audience
- Published
- 2010
93. Approche transcriptomique de l’acquisition de la fertilité du spermatozoïde chez le verrat
- Author
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Guyonnet, Benoit, Dacheux, Jean-Louis, Mercat, Marie-José, Schwob, Sandrine, Gatti, Jean-Luc, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut du Porc (IFIP), Interactions Biotiques et Santé Végétale, Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
- Subjects
épididyme ,BIOCHIMIE ,expression génique ,pcr ,[SDV]Life Sciences [q-bio] ,fertilité animale ,reproduction animale ,mammifère ,[INFO]Computer Science [cs] ,porcin ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2010
94. Maturation des spermatozoïdes dans l'épididyme: un nouveau regard sur un problème ancien
- Author
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Dacheux, Jean-Louis, Gatti, Jean-Luc, Dacheux, Françoise, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Interactions Biotiques et Santé Végétale, Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), ProdInra, Archive Ouverte, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV] Life Sciences [q-bio] ,FERTILITE ,[SDV]Life Sciences [q-bio] ,MATURATION ,SPERMATOZOIDE ,EPIDIDYME - Abstract
La compréhension des mécanismes cellulaires et moléculaires que constitue la maturation épididymaire des spermatozoïdes est depuis de nombreuses années un enjeu dans le domaine de la fertilité mâle. C'est l'espoir de pouvoir intervenir efficacement pour traiter certaines fertilités chez l'homme sans recours à des techniques de procréations assistées. Dans le domaine de la reproduction animale, c'est la possibilité de trouver de nouveaux marqueurs de prédiction pour sélectionner les meilleurs animaux reproducteurs. Chez l'homme, c'est de trouver un moyen de contraception masculine non hormonal. Ce domaine de recherche a bénéficié des progrès technologiques spectaculaires de cette dernière décennie en biologie comme le séquençage des génomes, l'obtention d'animaux transgéniques et les analyses à haut débit comme la transcriptomique et la protéomique. D'importantes avancées dans la connaissance des modifications post-testiculaires des gamètes et de leurs environnements épididymaires ont été obtenues. Bien que ces différenciations gamétiques existent chez tous les mammifères (monotrèmes compris), il est évident maintenant que ces différenciations sont spécifiques de l'espèce. Grâce à ces nouvelles approches, il est possible que l'on soit capable prochainement, si l'on s'en donne les moyens, d'être capable de pouvoir contrôler le pouvoir fécondant des spermatozoïdes.
- Published
- 2010
95. Conservation de la semence ovine
- Author
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Druart, Xavier, Guérin, Yvon, Gatti, Jean-Luc, Dacheux, Jean-Louis, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences - Abstract
National audience; In 2007, 43% of ovine dairy flocks and 4.3% of suckling flocks, respectively, 636 000 and 202 000 ewes, were inseminated. Methods of ram semen preservation in liquid state were adequate when insemination was performed the day of the semen collection in a reduced geographic region. However, given that insemination needs to be performed in the 10 hours following the semen collection in order to reach high fertility, increasing the duration of semen storage could simplify the daily work of Al Centers and provide better use of rams of high genetic value. Research is being conducted on three methods of preservation: liquid state, freezing and encapsulation. Several factors potentially impacting on fertility after semen preservation were identified such as final sperm concentration and antioxidants. Semen preservation also induced a reduced ability of spermatozoa to cross the cervix and transit into the female genital tract. A better understanding of the mechanisms involved in sperm transit could help to conceive media better suited to sperm preservation.; L’insémination ovine représente en 2007 43% du cheptel en races laitières et 4,3% en races allaitantes soit, respectivement, 636 000 et 202 000 inséminations. Les méthodes actuelles de conditionnement de la semence ovine sous forme liquide sont bien adaptées pour la réalisation des IA le jour de la collecte dans un secteur géographique réduit. Cependant, l’insémination devant être réalisée dans les 10 heures qui suivent la collecte pour obtenir une fertilité maximale, un allongement de la durée de conservation permettrait d’assouplir l’organisation de ces CIA et d’élargir la diffusion de la semence de béliers de haute valeur génétique. Les recherches portent sur trois méthodes de conservation : sous forme liquide, congelée ou encapsulée. Plusieurs facteurs influant sur la qualité de la semence après conservation ont été identifiés, comme la concentration finale en spermatozoïdes de la semence diluée et la présence d’antioxydants dans le milieu de conservation. La perte de fertilité après conservation se traduit également par une réduction de l’aptitude des spermatozoïdes à traverser le cervix et transiter dans le tractus génital femelle. Une meilleure compréhension des mécanismes mis en jeu lors de la traversée du cervix par les spermatozoïdes devrait permettre d’apporter des solutions adaptées pour améliorer les milieux de conservation.
- Published
- 2009
96. Transcriptome épididymaire et testiculaire chez le verrat
- Author
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Gatti, Jean-Luc, ProdInra, Migration, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
- Subjects
MATURATION EPIDIDYMAIRE ,[SDV] Life Sciences [q-bio] ,MALE ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,[INFO] Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2009
97. Etude in vivo par endoscopie à fluorescence du transport et de la survie des spermatozoïdes de bélier dans le tractus génital femelle
- Author
-
Druart, Xavier, Cognie, Juliette, Baril, Gérard, Clement, F., Dacheux, Jean-Louis, Gatti, Jean-Luc, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,ENDOSCOPIE A FLUORESCENCE ,[INFO] Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2008
98. Le transcriptome épididymaire du verrat : étude de la régionalisation
- Author
-
Guyonnet, Benoit, Dacheux, Jean-Louis, Jaffrézic, Florence, Lacoste, A., Marot, Guillemette, Mercat, M.J., Schwob, Sandrine, Gatti, Jean-Luc, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Station de Génétique Quantitative et Appliquée (SGQA), Institut National de la Recherche Agronomique (INRA), Institut du Porc (IFIP), ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,[INFO] Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2008
99. Analyse du transcriptome épididymaire chez le verrat
- Author
-
Guyonnet, Benoit, Dacheux, Jean-Louis, Jaffrézic, Florence, Lacoste, A., Marot, Guillemette, Mercat, M.J., Schwob, Sandrine, Gatti, Jean-Luc, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut du Porc (IFIP), Station de Génétique Quantitative et Appliquée (SGQA), Institut National de la Recherche Agronomique (INRA), ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,[INFO] Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2007
100. Identification directe de protéases par spectrométrie de masse après zymographie avec substrats fluorescents en gels d'électrophorèse mono et bidimensionnels
- Author
-
Thimon, V., Belghazi, Maya, Dacheux, Jean-Louis, Gatti, Jean-Luc, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,[INFO] Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2007
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