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64. TgPL2, a patatin-like phospholipase domain-containing protein, is involved in the maintenance of apicoplast lipids homeostasis in Toxoplasma.

Author

Lévêque, MF, Berry, L, Yamaryo-Botté, Y, Nguyen, HM, Galera, M, Botté, CY, Besteiro, S, Lévêque, MF, Berry, L, Yamaryo-Botté, Y, Nguyen, HM, Galera, M, Botté, CY, and Besteiro, S

Abstract

Patatin-like phospholipases are involved in numerous cellular functions, including lipid metabolism and membranes remodeling. The patatin-like catalytic domain, whose phospholipase activity relies on a serine-aspartate dyad and an anion binding box, is widely spread among prokaryotes and eukaryotes. We describe TgPL2, a novel patatin-like phospholipase domain-containing protein from the parasitic protist Toxoplasma gondii. TgPL2 is a large protein, in which the key motifs for enzymatic activity are conserved in the patatin-like domain. Using immunofluorescence assays and immunoelectron microscopy analysis, we have shown that TgPL2 localizes to the apicoplast, a non-photosynthetic plastid found in most apicomplexan parasites. This plastid hosts several important biosynthetic pathways, which makes it an attractive organelle for identifying new potential drug targets. We thus addressed TgPL2 function by generating a conditional knockdown mutant and demonstrated it has an essential contribution for maintaining the integrity of the plastid. In absence of TgPL2, the organelle is rapidly lost and remaining apicoplasts appear enlarged, with an abnormal accumulation of membranous structures, suggesting a defect in lipids homeostasis. More precisely, analyses of lipid content upon TgPL2 depletion suggest this protein is important for maintaining levels of apicoplast-generated fatty acids, and also regulating phosphatidylcholine and lysophosphatidylcholine levels in the parasite.

Published
2017

66. Autologous bone marrow mononuclear cells in the treatment of chronic limb-threatening ischaemia: a proof-of-concept trial.

Author

Quevedo, A Chacon, Garcia, J Garcia-Revillo, Galera, M Sagrario Lombardo, Lopez, M Canis, Carvajal, I Munoz, Martin-Palanco, V, Gomez, D De Alcala Martinez, Rospide, V Garcia, Palomino, J Patricio Linares, Aleman, A Capel, Llorente, C Castilla, Vallecillos, P Perez, Carrillo, M Angeles Moreno, Ruiz, F Tadeo Gomez, and Arroyo, C Herrera

Subjects
BONE marrow cells, BONE marrow examination, REVASCULARIZATION (Surgery), ISCHEMIA, BODY mass index, BONE marrow, GANGRENE
Abstract

Funding Acknowledgements Type of funding sources: Public hospital(s). Main funding source(s): 1. Iniciativa Andaluza en Terapias Avanzadas 2. Fundación Progreso y Salud Background Chronic limb threatening ischaemia (CLTI) is a debilitating condition often leading to gangrene which necessitates amputation. Endovascular or surgical interventions remain the standard treatment, however, not all patients are suitable for such procedures. The aim of cell-based therapies is to harvest progenitor cells either from the patient (i.e. autologous) or other sources and re-administer them at a higher concentration near/at the ischemic site(s) to potentiate vasculogenesis. Aim The objective of this trial was to assess the efficacy, safety, and feasibility of treating CLTI with autologous bone marrow mononuclear cells (BM-MNC). Methods This multicentre, phase II, open-label, randomized clinical trial conducted between 2009 and 2011 consisted of adult diabetic patients with CLTI Rutherford grade II or III of at least one lower limb who were not suitable for surgical revascularization. Sixty eligible patients were randomly assigned to one of three treatment groups (1x108, or 5x108, or 1x109 BM-MNCs) or to the control group (standard of care treatment). The cell-product was prepared from the baseline bone marrow aspirate of the treated patients and injected intra-arterially proximal to the vessel occlusion under general anaesthesia <72 hours after bone marrow aspiration. The control patients did not undergo bone marrow aspiration. Ulcer healing and amputation variables were evaluated in all patients at baseline and months 1, 3, 6, 9 and 12. Results The sample demographics were similar in each arm. A total of 50 males and 10 females were enrolled into the trial, with mean age of 63.9 (SD=9.4) years, and mean body mass index of 27.8 Kg/m2 (SD=4.1). The average years living with diabetes was 18.6 years, and time since diagnosis of CLTI was 0.78 years (SD: 1.12 years). A clinically meaningful treatment effect was observable at 6 months, and at 12 months baseline ulcers in 75% of treated patients were completely healed, compared to 14% of control patients (P=0.0142). Furthermore, the ulcers of the treated patients were on average 19.66 mm smaller than those of the control patients (P<0.0001). These results corresponded to angiographical outcomes where vasculogenesis was observed in approximately half of the treated patients who improved in Rutherford Grade. There were no differences in amputation rates across arms. No pertinent covariates which might have impacted the outcomes were identified. No treatment-associated adverse events occurred in the trial. Conclusion Autologous BM-MNC is an effective therapeutic alternative in patients unsuitable for surgical or endovascular revascularization. Open in new tab Download slide Table 1 Response to treatment Open in new tab Download slide Figure 1 Evolution of Rutherford grade [ABSTRACT FROM AUTHOR]

Published
2022
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68. Characteristics of the intertidal flat associated with Mira channel (inner domain of the Aveiro island-barrier complex, Portugal)

Author

Corrochano, A., Galera, M., Bernardes, C. A., and Barba, Pedro

Subjects
Portugal, Tidal channel, Intertidal flats, Linear bars, Barrier-island
Abstract

The Mira channel, located in the inner domain of the Aveiro barrier-island complex, is subdivided in three sections: head, intermediate and mouth, according to their morphological and sedimentary features. Their study shows the zonation of the associated intertidal flat in five zones: sand flat, lower mud flat, mixed flat, upper mud flat and marsh, related to different tidal levels

Published
1997

69. Características de la llanura intermareal asociada al canal de Mira (dominio interno del complejo isla-barrera de Aveiro, Portugal)

Author

Corrochano, A., Galera, M., Bernardes, C. A., and Barba, Pedro

Subjects
Portugal, Tidal channel, Intertidal flats, Linear bars, Barrier-island
Abstract

The Mira channel, located in the inner domain of the Aveiro barrier-island complex, is subdivided in three sections: head, intermediate and mouth, according to their morphological and sedimentary features. Their study shows the zonation of the associated intertidal flat in five zones: sand flat, lower mud flat, mixed flat, upper mud flat and marsh, related to different tidal levels

Published
1997

71. Polidactilia pós-axial e miopia progressiva

Author

Moura,Rita Cristina M. R., Perez,Ana Beatriz A., Erwenne,Clélia M., Galera,M. F., and Brunoni,Décio

Subjects
Polidactilia pós-axial, Miopia progressiva
Abstract

RESUMO O presente trabalho apresenta três casos de associação de miopia progressiva com polidactilia pós-axial, sendo que dois são irmãos do sexo masculino, filhos de casal não consanguíneo e o terceiro caso é de uma menina filha de pais consanguíneos. Verificou-se na literatura apenas um relato, de Czeizel e Brooser, em 1986, em que é descrita a mesma associação de defeitos, transmitindo-se em quatro gerações de forma autossômica dominante. Acreditamos que nossos pacientes apresentam a mesma síndrome, porém nestas duas famílias existem indícios de que a transmissão ocorra de forma autossômica recessiva.

Published
1994

76. Resolution of multicomponent peaks by multicomponent projection approach, positive matrix factorization and alternating least squares

Author

Frenich, Garrido, Galera, M. Martínez, Vidal, J.L.Martínez, Massart, Désiré Luc, Torres-Lapasío, J.R., De Braekeleer, Kris, Wang, Ji-Hong, Hopke, P.K., Frenich, Garrido, Galera, M. Martínez, Vidal, J.L.Martínez, Massart, Désiré Luc, Torres-Lapasío, J.R., De Braekeleer, Kris, Wang, Ji-Hong, and Hopke, P.K.

Abstract

info:eu-repo/semantics/published

Published
2000

90. Media Prosumers. Participatory Culture of Audiences and Media Responsability.

Author

García-Galera, M. Carmen and Valdivia, Angharad

Subjects
*SOCIAL action, *ONLINE social networks, *TECHNOLOGY & society
Abstract

An introduction to the journal is presented in which the editor discusses various reports published within the issue including one by Rosa Garcia Ruiz and colleagues on the positive impact of technology and the internet on people's lives and another by Carmen Garcia Galera and colleagues on young people's use of online social networks to participate in collective social action.

Published
2014
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