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3,782 results on '"GU Wen"'

59. TGFβ superfamily signaling regulates the state of human stem cell pluripotency and capacity to create well-structured telencephalic organoids

Author

Watanabe, Momoko, Buth, Jessie E, Haney, Jillian R, Vishlaghi, Neda, Turcios, Felix, Elahi, Lubayna S, Gu, Wen, Pearson, Caroline A, Kurdian, Arinnae, Baliaouri, Natella V, Collier, Amanda J, Miranda, Osvaldo A, Dunn, Natassia, Chen, Di, Sabri, Shan, de la Torre-Ubieta, Luis, Clark, Amander T, Plath, Kathrin, Christofk, Heather R, Kornblum, Harley I, Gandal, Michael J, and Novitch, Bennett G

Subjects
Biochemistry and Cell Biology, Biological Sciences, Stem Cell Research, Stem Cell Research - Embryonic - Human, Stem Cell Research - Induced Pluripotent Stem Cell, Stem Cell Research - Induced Pluripotent Stem Cell - Human, Neurosciences, 1.1 Normal biological development and functioning, Neurological, Generic health relevance, Cell Differentiation, Humans, Organoids, Pluripotent Stem Cells, Telencephalon, Transforming Growth Factor beta, brain organoid, cerebral cortex, choroid plexus, differentiation, embryonic stem cell, ganglionic eminence, hippocampus, neural development, neural stem cell, neurogenesis, pluripotency, pluripotent stem cell, stem cell heterogeneity, Clinical Sciences, Biochemistry and cell biology
Abstract

Telencephalic organoids generated from human pluripotent stem cells (hPSCs) are a promising system for studying the distinct features of the developing human brain and the underlying causes of many neurological disorders. While organoid technology is steadily advancing, many challenges remain, including potential batch-to-batch and cell-line-to-cell-line variability, and structural inconsistency. Here, we demonstrate that a major contributor to cortical organoid quality is the way hPSCs are maintained prior to differentiation. Optimal results were achieved using particular fibroblast-feeder-supported hPSCs rather than feeder-independent cells, differences that were reflected in their transcriptomic states at the outset. Feeder-supported hPSCs displayed activation of diverse transforming growth factor β (TGFβ) superfamily signaling pathways and increased expression of genes connected to naive pluripotency. We further identified combinations of TGFβ-related growth factors that are necessary and together sufficient to impart broad telencephalic organoid competency to feeder-free hPSCs and enhance the formation of well-structured brain tissues suitable for disease modeling.

Published
2022

75. Electron transport chain inhibition increases cellular dependence on purine transport and salvage

Author

Wu, Zheng, Bezwada, Divya, Cai, Feng, Harris, Robert C., Ko, Bookyung, Sondhi, Varun, Pan, Chunxiao, Vu, Hieu S., Nguyen, Phong T., Faubert, Brandon, Cai, Ling, Chen, Hongli, Martin-Sandoval, Misty, Do, Duyen, Gu, Wen, Zhang, Yuanyuan, Zhang, Yuannyu, Brooks, Bailey, Kelekar, Sherwin, Zacharias, Lauren G., Oaxaca, K. Celeste, Patricio, Joao S., Mathews, Thomas P., Garcia-Bermudez, Javier, Ni, Min, and DeBerardinis, Ralph J.

Published
2024
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88. BAlN for III-nitride UV light emitting diodes: undoped electron blocking layer

Author

Gu, Wen, Lu, Yi, Lin, Rongyu, Guo, Wenzhe, Zhang, Zi-Hui, Ryou, Jae-Hyun, Yan, Jianchang, Wang, Junxi, Li, Jinmin, and Li, Xiaohang

Subjects
Physics - Applied Physics
Abstract

The undoped BAlN electron-blocking layer (EBL) is investigated to replace the conventional AlGaN EBL in light-emitting diodes (LEDs). Numerical studies of the impact of variously doped EBLs on the output characteristics of LEDs demonstrate that the LED performance shows heavy dependence on the p-doping level in the case of the AlGaN EBL, while it shows less dependence on the p-doping level for the BAlN EBL. As a result, we propose an undoped BAlN EBL for LEDs to avoid the p-doping issues, which a major technical challenge in the AlGaN EBL. Without doping, the proposed BAlN EBL structure still possesses a superior capacity in blocking electrons and improving hole injection compared with the AlGaN EBL having high doping. This study provides a feasible route to addressing electron leakage and insufficient hole injection issues when designing UV LED structures.

Published
2020

91. Information Gerrymandering in Elections

Author

Liu, Xiaoxue, Kato, Shohei, Ren, Fenghui, Su, Guoxin, Zhang, Minjie, Gu, Wen, Goos, Gerhard, Founding Editor, Hartmanis, Juris, Founding Editor, Bertino, Elisa, Editorial Board Member, Gao, Wen, Editorial Board Member, Steffen, Bernhard, Editorial Board Member, Yung, Moti, Editorial Board Member, Wu, Shiqing, editor, Yang, Wenli, editor, Amin, Muhammad Bilal, editor, Kang, Byeong-Ho, editor, and Xu, Guandong, editor

Published
2023
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95. A Developmental Anti-collision Architecture for the Brain-Like Event-Related Potential Cycle in UAVs

Author

Zhang, Qi-rui, Wei, Rui-xuan, Luo, Wei-er, Gu, Wen-yuan, Wang, Zhe-zong, Angrisani, Leopoldo, Series Editor, Arteaga, Marco, Series Editor, Panigrahi, Bijaya Ketan, Series Editor, Chakraborty, Samarjit, Series Editor, Chen, Jiming, Series Editor, Chen, Shanben, Series Editor, Chen, Tan Kay, Series Editor, Dillmann, Rüdiger, Series Editor, Duan, Haibin, Series Editor, Ferrari, Gianluigi, Series Editor, Ferre, Manuel, Series Editor, Hirche, Sandra, Series Editor, Jabbari, Faryar, Series Editor, Jia, Limin, Series Editor, Kacprzyk, Janusz, Series Editor, Khamis, Alaa, Series Editor, Kroeger, Torsten, Series Editor, Li, Yong, Series Editor, Liang, Qilian, Series Editor, Martín, Ferran, Series Editor, Ming, Tan Cher, Series Editor, Minker, Wolfgang, Series Editor, Misra, Pradeep, Series Editor, Möller, Sebastian, Series Editor, Mukhopadhyay, Subhas, Series Editor, Ning, Cun-Zheng, Series Editor, Nishida, Toyoaki, Series Editor, Oneto, Luca, Series Editor, Pascucci, Federica, Series Editor, Qin, Yong, Series Editor, Seng, Gan Woon, Series Editor, Speidel, Joachim, Series Editor, Veiga, Germano, Series Editor, Wu, Haitao, Series Editor, Zamboni, Walter, Series Editor, Zhang, Junjie James, Series Editor, Yan, Liang, editor, and Deng, Yimin, editor

Published
2023
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100. Vaccination with dengue virus-like particles induces humoral and cellular immune responses in mice

Author

Zhang Quanfu, Zhang Fushun, Zhang Li, Jin Cong, Wang Xiaofang, Miao Fang, Li Chuan, Gu Wen, Liang Mifang, Zhang Shuo, Jiang Lifang, Li Mengfeng, and Li Dexin

Subjects
Dengue virus, VLP, Vaccine, Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background The incidence of dengue, an infectious disease caused by dengue virus (DENV), has dramatically increased around the world in recent decades and is becoming a severe public health threat. However, there is currently no specific treatment for dengue fever, and licensed vaccine against dengue is not available. Vaccination with virus-like particles (VLPs) has shown considerable promise for many viral diseases, but the effect of DENV VLPs to induce specific immune responses has not been adequately investigated. Results By optimizing the expression plasmids, recombinant VLPs of four antigenically different DENV serotypes DENV1-4 were successfully produced in 293T cells. The vaccination effect of dengue VLPs in mice showed that monovalent VLPs of each serotype stimulated specific IgG responses and potent neutralizing antibodies against homotypic virus. Tetravalent VLPs efficiently enhanced specific IgG and neutralizing antibodies against all four serotypes of DENV. Moreover, vaccination with monovalent or tetravalent VLPs resulted in the induction of specific cytotoxic T cell responses. Conclusions Mammalian cell expressed dengue VLPs are capable to induce VLP-specific humoral and cellular immune responses in mice, and being a promising subunit vaccine candidate for prevention of dengue virus infection.

Published
2011
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