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[Aim]: To investigate the effects of egg white hydrolysate (EWH) on the lipid and glycemic metabolism disruption in the white adipose tissue (WAT) dysfunction induced by mercury (Hg). Experimental: Wistar rats were treated for 60 days: control (saline, intramuscular – i.m.); hydrolysate (EWH, gavage, 1 g kg−1 day−1); mercury (HgCl2, i.m., 1st dose 4.6 μg kg−1, subsequent doses 0.07 μg kg−1 day−1) and hydrolysate-mercury (EWH-HgCl2). Hg level and histological analyses were performed in epididymal WAT (eWAT), pancreas and liver. GRP78, CHOP, PPARα, PPARγ, leptin, adiponectin, and CD11 mRNA expressions were analyzed in eWAT. The plasma lipid profile, glucose, and insulin levels were measured. Antioxidant status was also evaluated in the plasma and liver., [Results]: EWH intake prevented the reduced eWAT weight, adipocyte size, insulin levels, and antioxidant defenses and the increased glucose and triglyceride levels induced by Hg exposure; hepatic glutathione levels were higher in rats co-treated with EWH. The increased mRNA expression of CHOP, PPARα, and leptin induced by Hg was reduced in co-treated rats. EWH did not modify the elevated mRNA expression of GRP78, PPARγ and adiponectin in Hg-treated rats. Increased levels of Hg were found in the liver; the co-treatment did not alter this parameter. EWH prevented the morphological and metabolic disorder induced by Hg, by improving antioxidant defenses, inactivating pro-apoptotic pathways and normalizing the mRNA expression of PPARs and adipokines. Its effects enabled an increase in insulin levels and a normal balance between the fat storage and expenditure mechanisms in WAT., [Conclusions]: EWH may have potential benefits in the prevention and management of Hg-related metabolic disorders., This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq [203440/2014-5; 44181/2014-9; 307399/2017-6]; Fundação de Amparo à Pesquisa do Rio Grande do Sul – FAPERGS [PQG:19/2551-0001810-0]; Fundação de Amparo à Pesquisa e Inovação do Espírito Santo – FAPES [No 80598773] and the Agencia Estatal de Investigación (AEI) and Fondo Europeo de Desarrollo Regional (FEDER) [AGL2017-89213].

Ground-based and airborne instrumentation for astronomy IX (2022), Montreal, JUL 17-22, 2022.--Proceedings of SPIE - The International Society for Optical Engineering vol. 12184 Article number 121840M.-- Complete list of authors: Marin-Franch, A.; Rueda-Teruel, S.; Lopez Alegre, G.; Iniguez, C.; Vazquez Ramio, H.; Ederoclite, A.; Bello, R.; Royo-Navarro, M.; Casino-Martin, J. M.; Lozano-Perez, D.; Molina-Ibanez, E. L.; Rueda-Teruel, F.; Yanes-Diaz, A.; del Pino, A.; Lopez-Sanjuan, C.; Cenarro, A. J.; Cristobal-Hornillos, D.; Hernan-Caballero, A.; Moles, M.; Varela, J.; Bielsa de Toledo, S.; Chueca, S.; Domingez-Martinez, M.; Garces-Cubel, D.; Martinez-Olivar, N.; Munoz-Maudos, J.; Rueda-Asensio, H.; Soriano-Laguia, I.; Castillo, J.; Civera, T.; Hernandez-Fuertes, J.; Lopez-Sainz, A.; Moreno-Signes, A.; Muniesa-Gallardo, D.; Taylor, K.; Santoro, F.; Cepa, J.; Fermino, C.; Bastable, M.; Haddow, G.; Palmer, I.; Simpson, C.; Spatola, A.; Sweeney, H.; Tatard, C.; Watkins, M.; Brauneck, U.; Casalta, J. M.; Abramo, R.; Alcaniz, J.; Benitez, N.; Bonoli, S.; Carneiro, S.; Dupke, R.; Mendes de Oliveira, C.; Sodre, L., Jr.; Vilchez, J., Commissioning results, on-sky performance and first operations of the Javalambre Panoramic Camera (JPCam) are presented in this paper. JPCam is a 1.2 Gpixel camera deployed on the 2.6m, large field-of-vie Javalambre Survey Telescope (JST250) at the Observatorio Astrofisico de Javalambre. JPCam has been conceived to perform J-PAS, a photometric survey of several thousand square degrees of the northern sky in 56 optical bands, 54 of them narrow-band filters (145 angstrom FWHM), contiguous and equi-spaced between 370 and 920nm, producing a low resolution photo-spectrum of every pixel of the observed sky, hence promising crucial breakthroughs in Cosmology and galaxy formation and evolution. JPCam has been designed to maximize field-of-view and wavelength coverage while guaranteeing a high image quality over the entire focal plane. To this aim, JPCam is equipped with a mosaic of 14 9.2k x 9.2k, 10 mu m pixel, low noise detectors from Teledyne-E2V, providing a FoV of 4.1 square degrees with a plate scale of 0.2267''/pix. In full frame mode, camera electronics allows read times of 10.9s at 633kHz read frequency (16.4s at 400kHz) with a readout noise of 5.5e(-) (4.3e(-)). Its filter unit admits 5 filter trays, each mounting 14 filters corresponding to the 14 CCDs of the mosaic and allowing all the J-PAS filters to be permanently installed. To fully optimize image quality, position of JST250 secondary mirror and JPCam focal plane are maintained optically aligned by means of two hexapod systems. To perform this task, JPCam includes 12 auxiliary detectors, 4 for autoguiding and 8 for image quality control through wavefront sensing., Based on observations made with the JST250 telescope and JPCam at the Observatorio Astrofisico de Javalambre (OAJ), in Teruel, owned, managed, and operated by the Centro de Estudios de Fisica del Cosmos de Aragon (CEFCA). We acknowledge the OAJ Data Processing and Archiving Unit (UPAD) for reducing and calibrating the OAJ data used in this work. Funding for the J-PAS Project has been provided by the Governments of Spain and Aragon through the Fondo de Inversion de Teruel, European FEDER funding and the Spanish Ministry of Science, Innovation and Universities, and by the Brazilian agencies FINEP, FAPESP, FAPERJ and by the National Observatory of Brazil. Additional funding was also provided by the Tartu Observatory and by the J-PAS Chinese Astronomical Consortium., With funding from the Spanish government through the Severo Ochoa Centre of Excellence accreditation SEV-2017-0709

This work aimed to predict C18:1 TFA isomers as well as other groups of fatty acids (saturated, monounsaturated, polyunsaturated, and total TFA) in chocolates by ATR-FTIR and partial least square regression. The quantification of fatty acids in representative samples (white, dark, and milk chocolates) was rapid (, This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001 and PDSE 88881187078/2018-01; Spanish Ministry of Economy and Competitiveness (AGL2016-75159-C2-2-R); CNPq (303355/2017-4, 424032/2018-0); Finep (CT-INFRA 01/2013-REF 0633/13); RQ-MG (CEX.RED- 00010-14); and INCTBio (FAPESP 2014/50867-3, CNPq 465389/2014-7).

Nitric oxide (NO) is a multifunctional gaseous signal that modulates the growth, development and stress tolerance of higher plants. NO donors have been used to boost plant endogenous NO levels and to activate NO-related responses, but this strategy is often hindered by the relative instability of donors. Alternatively, nanoscience offers a new, promising way to enhance NO delivery to plants, as NO-releasing nanomaterials (e.g. S-nitrosothiol-containing chitosan nanoparticles) have many beneficial physicochemical and biochemical properties compared to non-encapsulated NO donors. Nano NO donors are effective in increasing tissue NO levels and enhancing NO effects both in animal and human systems. The authors believe, and would like to emphasize, that new trends and technologies are essential for advancing plant NO research and nanotechnology may represent a breakthrough in traditional agriculture and environmental science. Herein, we aim to draw the attention of the scientific community to the potential of NO-releasing nanomaterials in both basic and applied plant research as alternatives to conventional NO donors, providing a brief overview of the current knowledge and identifying future research directions. We also express our opinion about the challenges for the application of nano NO donors, such as the environmental footprint and stakeholder's acceptance of these materials., NO research in the Kolbert lab is financed by NRDI Office (grant no. K135303). NO research in the Seabra lab is financed by Fapesp (2018/08194-2, 2020/03646-2) and CNPq (404815/2018-9, 313117/2019-5). NO research in the Oliveira’s lab is financed by Fundacao Araucaria de Apoio ao Desenvolvimento Científico do Parana (18.017.997-6), Fapesp (2019/15095-3) and CNPq (311034/2020-9). NMS and RVR acknowledge the financial support by Fapesp (2012/19167-0, 2015/21546-7, 2017/11279- 7, 2018/08194-2, 2020/03646-2), CNPq (302460/2018–7) and National Program of Post-Doctorate (PNPD, Capes). Research by FJC and JMP is supported by a European Regional Development Fund cofinanced from the Ministry of Economy and Competitiveness (PID 2019-103924 GB-I00) and Junta de Andalucía, Spain.

P-type and n-type metal oxide semiconductors are widely used in the manufacture of gas sensing materials, due to their excellent electronic, electrical and electrocatalytic properties. Hematite (α-Fe2O3) compound has been reported as a promising material for sensing broad types of gases, due to its affordability, good stability and semiconducting properties. In the present work, the efficient and easy-to-implement sol-gel method has been used to synthesize α-Fe2O3 nanoparticles (NPs). The TGA-DSC characterizations of the precursor gel provided information about the phase transformation temperature and the mass percentage of the hematite NPs. X-ray diffraction, transmission electron microscopy and x-ray photoelectron spectroscopy data analyses indicated the formation of two iron oxide phases (hematite and magnetite) when the NPs are subjected to thermal treatment at 400 °C. Meanwhile, only the hematite phase was determined for thermal annealing above 500 °C up to 800 °C. Besides, the crystallite size shows an increasing trend with the thermal annealing and no defined morphology. A clear reduction of surface defects, associated with oxygen vacancies was also evidenced when the annealing temperature was increased, resulting in changes on the electrical properties of hematite NPs. Resistive gas-sensing tests were carried out using hematite NPs + glycerin paste, to detect quaternary ammonium compounds. Room-temperature high sensitivity values (Sr ∼ 4) have been obtained during the detection of ∼1 mM quaternary ammonium compounds vapor. The dependence of the sensitivity on the particle size, the mass ratio of NPs with respect to the organic ligand, changes in the dielectric properties, and the electrical conduction mechanism of gas sensing was discussed., This research work was carried out with the financial support provided by CONCYTEC-PROCIENCIA within the framework of the call E038-01 [Contract N°07-2019-FONDECYT-BM-INC.INV]. JAHC wants to thank the Brazilian agencies CNPq (Grants number 443652/2018–0, 303182/2020–2) and FAPDF (grant number 00193.0000151/2019–20) for the financial support.

Parental care is a major feature in the evolution of life- history traits and appears highly associated with the trade-off between egg number and size. It has been a controversial issue in studies about reproductive strategy and is deemed to have evolved multiple times, both in aquatic and terrestrial taxa. To explore relationships between parental care and egg size, we examined 313 species of bony fishes, including 152 species with parental care and 161 species without parental care. We mapped parental care onto a maximum likelihood (ML) unrooted phylogeny and analysed egg size and numbers data set using a logistic regression. The phylogenetically controlled analyses revealed that parental care is dispersed along with the retrieved phylogeny and that at least four distinct lineages parallel evolved from non- parental care lineages to parental care ones. The results of the logistic regression highlighted that egg size can be used as a good predictor of parental care and that there is a strong and consistent threshold in egg size transition between parental and non- parental care fishes. We conclude that egg size predicts parental care in bony fish and that egg size distribution is polymodal within parental care species probably reflecting the variation in the amount of energy used for care. To advance the knowledge about the hypotheses raised in this study, it would be important to gather information about more species and their respective number and size of eggs and the presence/absence of parental care., The project was supported by FAPERJ E-26/201.583/2018 to RIR; by CNPq 301463/2017-4, FAPERJ CNE E-26/202.762/2018, Prociência/UERJ/SR2 and Capes PrInt 88887.511123/2020-00 to RM and CNPq to CRLA.

Extragalactic astronomy., [Context] The Lyα emission is an important tracer of neutral gas in a circum-galactic medium (CGM) around high-z quasi-stellar objects (QSOs). The origin of Lyα emission around QSOs is still under debate, bringing on significant implications for galaxy formation and evolution., [Aims] In this paper, we study Lyα nebulae around two high redshift QSOs, SDSS J141935.58+525710.7 at z = 3.218 (hereafter QSO1) and SDSS J141813.40+525240.4 at z = 3.287 (hereafter QSO2), from the miniJPAS survey within the All-wavelength Extended Groth Strip International Survey (AEGIS) field., [Methods] Using the contiguous narrow-band (NB) images from the miniJPAS survey and Sloan Digital Sky Survey (SDSS) spectra, we analyzed their morphology, nature, and origin., [Results] We report the serendipitous detection of double-core Lyα morphology around two QSOs, which is rarely seen among other QSOs. The separations of the two Lyα cores are 11.07 ± 2.26 kpcs (1.47 ± 0.3″) and 9.73 ± 1.55 kpcs (1.31 ± 0.21″), with Lyα line luminosities of ∼3.35 × 1044 erg s−1 and ∼6.99 × 1044 erg s −1 for QSO1 and QSO2, respectively. The miniJPAS NB images show evidence of extended Lyα and CIV morphology for both QSOs and extended HeII morphology for QSO1., [Conclusions] These two QSOs may be potential candidates for the new enormous Lyman alpha nebula (ELAN) found from the miniJPAS survey due to their extended morphology in the shallow depth and relatively high Lyα luminosities. We suggest that galactic outflows are the major powering mechanism for the double-core Lyα morphology. Considering the relatively shallow exposures of miniJPAS, the objects found here could merely form the cusp of a promising number of such objects that will be uncovered in the upcoming full Javalambre-Physics of the Accelerated Universe Astrophysical Survey (J-PAS) survey and deep integral field units (IFU) observations with 8–10 m telescopes will be essential for constraining the underlying physical mechanism that is responsible for the double-cored morphology., Z.Y.Z. acknowledges support by the National Science Foundation of China (12022303), the China-Chile Joint Research Fund (CCJRF No. 1906), and the CAS Pioneer Hundred Talents Program. RPT thanks the CAS President’s International Fellowship Initiative (PIFI) (Grant No. E085201009) for supporting this work. We acknowledge the science research grants from the China Manned Space Project with No. CMS-CSST-2021-A04 and CMS-CSST-2021-A07. ACS acknowledges funding from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and the Rio Grande do Sul Research Foundation (FAPERGS) through grants CNPq-11153/2018-6, CNPq-314301/2021-6 and FAPERGS/CAPES 19/2551-0000696-9 and the Chinese Academy of Sciences (CAS) President’s International Fellowship Initiative (PIFI) through grant E085201009. R.P.T. and Z.Y.Z. thanks Shuairu Zhu, Fang-Ting Yuan, Ruqiu Lin, and Xiang Ji for their useful discussion during the preparations of the manuscript. R.P.T. acknowledges Carolina Queiroz for sharing information about the JPAS database. This paper has gone through the internal review by the J-PAS collaboration. Based on observations made with the JST/T250 telescope and JP Cam at the Observatorio Astrofísico de Javalambre (OAJ), in Teruel, owned, managed, and operated by the Centro de Estudios de Física del Cosmos de Aragón (CEFCA). We acknowledge the OAJ Data Processing and Archiving Unit (UPAD) for reducing and calibrating the OAJ data used in this work. Funding for the J-PAS Project has been provided by the Governments of Spain and Aragón through the Fondo de Inversión de Teruel, European FEDER funding and the Spanish Ministry of Science, Innovation and Universities, and by the Brazilian agencies FINEP, FAPESP, FAPERJ and by the National Observatory of Brazil. Additional funding was also provided by the Tartu Observatory and by the J-PAS Chinese Astronomical Consortium. L.A.D.G., C.K., and R.G.D., acknowledge financial support from the State Agency for Research of the Spanish MCIU through the “Center of Excellence Severo Ochoa” award to the Instituto de Astrofísica de Andalucía (SEV-2017-0709), and to PID2019-109067-GB100.

Fruits are unique to flowering plants and confer a selective advantage as they facilitate seed maturation and dispersal. In fleshy fruits, development and ripening are associated with numerous structural, biochemical, and physiological changes, including modifications in the general appearance, texture, flavor, and aroma, which ultimately convert the immature fruit into a considerably more attractive and palatable structure for seed dispersal by animals. Treatment with exogenous nitric oxide (NO) delays fruit ripening, prevents chilling damage, promotes disease resistance, and enhances the nutritional value. The ripening process is influenced by NO, which operates antagonistically to ethylene, but it also interacts with other regulatory molecules such as abscisic acid, auxin, jasmonic acid, salicylic acid, melatonin, and hydrogen sulfide. NO content progressively declines during fruit ripening, with concomitant increases in protein nitration and nitrosation, two post-translational modifications that are promoted by reactive nitrogen species. Dissecting the intimate interactions of NO with other ripening-associated factors, including reactive oxygen species, antioxidants, and the aforementioned phytohormones, remains a challenging subject of research. In this context, integrative 'omics' and gene-editing approaches may provide additional knowledge of the impact of NO in the regulatory processes involved in controlling physiology and quality traits in both climacteric and non-climacteric fruits., Financing by ERDF funds through the Ministry of Economy and Competitiveness (AGL2015-65104-P) and by the Junta de Andalucía (group BIO192), Spain, is acknowledged. The work of LF is supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, grant nos #2016/01128-99 and #2018/16389-8).

Structural and electronic properties of bilayer graphene films and nanostructures obtained through the graphitization of SiC(0001) were investigated in this work using scanning tunneling microscopy and spectroscopy. We report on the observation of triangular nanostructures which result from extended stacking faults in the SiC substrate and their effects on graphene layers that are formed on top of them. Spectroscopic measurements revealed distinct electronic responses as a function of the local hydrogen intercalation. Spectroscopic signatures ranging from single- to double-layer graphene, as well as intermediate states were observed as a consequence of the (in)complete hydrogen intercalation process. High resolution topographic scanning tunneling microscopy images at resonant bias voltages inside triangular nanostructures reveal that the bottom layer of the bilayer graphene film is still bonded to the substrate. Therefore, the triangular nanostructures present edges and facets with the coexistence of carbon atoms in sp3 and sp2 hybridizations. Using atomistic calculations we have modeled the local density of states of these objects reproducing their electronic response. The generation of regions with distinct electronic responses is potentially interesting for high-density data storage with hidden bit capabilities., The authors acknowledge experimental support by the Brazilian Synchrotron Light Laboratory (LNLS). Financial support was provided by CNPq, CAPES, FAPEMIG and INCT-Nano-Carbono. This work was partially financed by the Project FIS2016-76617-P of the Spanish Ministry of Economy and Competitiveness MINECO, the Basque Government under the ELKARTEK project (SUPER), and the University of the Basque Country (Grant No. IT-756-13).

This article belongs to the Special Issue Recent Trends and Applications of Metabolomics in Food Analysis, Authentication and Process Monitoring., The valorization of agri-food by-products is essential from both economic and sustainability perspectives. The large quantity of such materials causes problems for the environment; however, they can also generate new valuable ingredients and products which promote beneficial effects on human health. It is estimated that soybean production, the major oilseed crop worldwide, will leave about 597 million metric tons of branches, leaves, pods, and roots on the ground post-harvesting in 2020/21. An alternative for the use of soy-related by-products arises from the several bioactive compounds found in this plant. Metabolomics studies have already identified isoflavonoids, saponins, and organic and fatty acids, among other metabolites, in all soy organs. The present review aims to show the application of metabolomics for identifying high-added-value compounds in underused parts of the soy plant, listing the main bioactive metabolites identified up to now, as well as the factors affecting their production., This research was funded by São Paulo Research Foundation—FAPESP, grant number 2020/09500-0, 2018/21128-9 and 2017/06216-6, and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001.

Gluten-free bread (GFB) is generally recognized as a product with a less than satisfying appearance, texture, and mouthfeel that lacks nutritional quality and has a short shelf life. In this sense, the potential of chickpea flour (CF), psyllium (PSY), and their combination at reducing GFB staling were investigated in comparison to control bread prepared with rice flour (RF) and cassava starch (75:25 flour weight basis). The effect of time on the crumb and crust moisture and texture and loaves sensory parameters (acceptability on a 9-point structured hedonic scale, Just-About-Right level for bread softness, sensory profile by Check-All-That-Apply) was assessed during 7 days of storage. Replacing RF with CF reduced moisture loss and crumb hardening not only on the same day of baking but also after 7 days of storage. PSY addition (5.5 % flour weight basis) delayed the loss of cohesiveness and springiness of crumb during storage. The findings suggest that CF, and its combination with PSY, was promising for reducing and delaying GFB staling, thus maintaining acceptability, softness, and freshness after 7 days of storage, according to the perceptions of consumers., This work was supported by a grant (2012/17838-4) from the São Paulo Research Foundation (FAPESP) and the CNPq - National Council for Scientific and Technological Development - Brazil (grant number 101025/2016-5).

[Background]: A high‐fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat. Technological and microbiological properties were evaluated for 60 days at 4 °C., [Results]: A reduction of 11 to 34% and 35 to 45% of fat and sodium were obtained in reformulated products, respectively. An increase in fiber content and polyunsaturated fatty acid was noticed in the formulations with EG. The addition of EG in Bologna increased L* (lightness) values and reduced a* (redness/greenness) values comparing to control treatment. Microstructural properties of sausages exhibited a denser network with the presence of EG. Softer, more elastic, cohesive and resilient samples with a higher intensity of lipid oxidation (P, [Conclusion]: These results suggest that inulin‐based EG is a potential fat substitute for developing healthier meat products, with better fatty acids composition and stable to chilled storage., The authors are thankful to CNPq (140 531/2015‐7), CAPES (140 531/2015‐7), and FAPESP (2016/19 967‐7) for providing financial support and scholarships that sustained this study.

HCN and its isomer HNC play an important role in molecular cloud chemistry and the formation of more complex molecules. We investigate here the impact of protostellar shocks on the HCN and HNC abundances from high-sensitivity IRAM 30m observations of the prototypical shock region L1157-B1 and the envelope of the associated Class 0 protostar, as a proxy for the pre-shock gas. The isotopologues H$^{12}$CN, HN$^{12}$C, H$^{13}$CN, HN$^{13}$C, HC$^{15}$N, H$^{15}$NC, DCN and DNC were all detected towards both regions. Abundances and excitation conditions were obtained from radiative transfer analysis of molecular line emission under the assumption of Local Thermodynamical Equilibrium. In the pre-shock gas, the abundances of the HCN and HNC isotopologues are similar to those encountered in dark clouds, with a HCN/HNC abundance ratio $\approx 1$ for all isotopologues. A strong D-enrichment (D/H$\approx 0.06$) is measured in the pre-shock gas. There is no evidence of $^{15}$N fractionation neither in the quiescent nor in the shocked gas. At the passage of the shock, the HCN and HNC abundances increase in the gas phase in different manners so that the HCN/HNC relative abundance ratio increases by a factor 20. The gas-grain chemical and shock model UCLCHEM allows us to reproduce the observed trends for a C-type shock with pre-shock density $n$(H)= $10^5$cm$^{-3}$ and shock velocity $V_s= 40$km/s. We conclude that the HCN/HNC variations across the shock are mainly caused by the sputtering of the grain mantle material in relation with the history of the grain ices., Comment: 14 pages, 7 figures. Accepted for publication in MNRAS

[Background]: Ralstonia solanacearum is the causal agent of bacterial wilt, a devastating plant disease responsible for serious economic losses especially on potato, tomato, and other solanaceous plant species in temperate countries. In R. solanacearum, gene expression analysis has been key to unravel many virulence determinants as well as their regulatory networks. However, most of these assays have been performed using either bacteria grown in minimal medium or in planta, after symptom onset, which occurs at late stages of colonization. Thus, little is known about the genetic program that coordinates virulence gene expression and metabolic adaptation along the different stages of plant infection by R. solanacearum., [Results]: We performed an RNA-sequencing analysis of the transcriptome of bacteria recovered from potato apoplast and from the xylem of asymptomatic or wilted potato plants, which correspond to three different conditions (Apoplast, Early and Late xylem). Our results show dynamic expression of metabolism-controlling genes and virulence factors during parasitic growth inside the plant. Flagellar motility genes were especially up-regulated in the apoplast and twitching motility genes showed a more sustained expression in planta regardless of the condition. Xylem-induced genes included virulence genes, such as the type III secretion system (T3SS) and most of its related effectors and nitrogen utilisation genes. The upstream regulators of the T3SS were exclusively up-regulated in the apoplast, preceding the induction of their downstream targets. Finally, a large subset of genes involved in central metabolism was exclusively down-regulated in the xylem at late infection stages., [Conclusions]: This is the first report describing R. solanacearum dynamic transcriptional changes within the plant during infection. Our data define four main genetic programmes that define gene pathogen physiology during plant colonisation. The described expression of virulence genes, which might reflect bacterial states in different infection stages, provides key information on the R. solanacearum potato infection process., This work was funded by project PID2019-108595RB-I00 from the Spanish Ministry of Economy and Competitiveness. We also acknowledge financial support from the “Severo Ochoa Program for Centres of Excellence in R&D” 2016–2019 (SEV-2015-0533) and the CERCA Program of the Catalan Government (Generalitat de Catalunya). R. de Pedro-Jové received FI and FPU fellowships from Generalitat de Catalunya and the Spanish Ministry of Economy and Competitiveness. P. Sebastià received the support of a fellowship (code is LCF/BQ/IN17/11620004) from la Caixa Foundation (identifier [ID] 100010434). This project has received funding from the European Union’s Horizon 2020 research and innovation program under Marie Skłodowska-Curie grant agreement no. 713673. M. Puigvert received an APIF doctoral fellowship from Universitat de Barcelona and travel fellowship funded by Fundació Montcelimar and Universitat de Barcelona to carry out a short stay in J.C. Setubal’s lab. J.C. Setubal holds a CNPq senior researcher fellowship. J.S. Monteiro is funded by a FAPESP doctoral fellowship under process number 2019/05287–2. A.P. Macho is funded by the Chinese Academy of Sciences and the Chinese 1000 Talents Program.

This work reports a pH-triggered release system based on core@shell mesoporous magnetic nanoparticles (MNP@mSiO2) obtained using a simple and rapid ultrasound-assisted method. Performed characterization reveals magnetic cores of Fe2.9Mn0.1O4 (38 ± 6 nm) and specific loss power values adequate for hyperthermia (463 W/g), surrounded by a mesoporous silica shell (10 ± 2 nm) with large surface area (269 m2 g-1) functionalized with hydroxyl groups (-OH). MNP@mSiO2 were loaded with DOX and amino-silane grops, providing pH-triggered DOX release at acidic environments, driving by dipolar intermolecular interactions. The experimental DOX release kinetics at pH 5.5, 6.6 and 7.4 were determined and adjusted to Gompertz dissolution model (Nash–Sutcliffe efficiency coefficient (NSE>0.9)), where the only strongly pH-dependent variable is the percentage of DOX released. The pH-triggered response observed in the system was ~20% of the DOX loaded into the MNP@mSiO2 is released at pH 6.6 or 7.4, whereas up to 80 wt% is released at pH 5.5. Time to 50% of release and dissociation rate of the system remaining constant, suggesting no-pH influence on these parameters. The biological assays highlight negligible hemolytic effect and cytocompatibility of the hybrid material, pointing out the potential use of MNP@mSiO2 as a magnetic driven drug delivery system with pH-triggered drug release kinetics at acidic environments. These results probe the feasibility of sonochemical methods in the elaboration of biocompatible and controlled properties nanomaterials for drug release applications, with the advantage of accurately responses predictions by mathematical model and using minimal processing steps or laboratory equipment., This work was partially supported by the Spanish Ministerio de Ciencia, Innovación y Universidades (project PID2019-106947RB-C21), FAPESP, Brazil (Grant 2014/50983-3) and CAPES, Brazil (Grant 88881.189090/2018-01). FCG acknowledges the financial support provided by FAPESP (Grant 2019/06634-8).

A modified 45S5 Bioglass® containing 2 mol% alumina and 2 mol% strontium oxide was prepared. In vitro bioactivity in powder and monolithic samples was conducted. Fourier transform infrared analysis and pH change in simulated body fluid were investigated for powder samples after 480 min of immersion. Additionally, X-ray diffraction and scanning electron microscopy after 7 and 14 days of immersion in simulated body fluid were investigated for monolithic samples. The formation of phosphate and carbonate phase precursors of hydroxycarbonate apatite was observed after 480 min for powder samples, and the hydroxycarbonate apatite pattern and morphology were confirmed after 7 days of immersion for monolithic samples. It seems that changes in composition did not affect reactivity but caused a decrease in the maxima of pH. Cytotoxicity and cell viability obtained by using the NCTC clone 929 cell line did not show any significant loss of cell viability or cytotoxicity. Moreover, after overnight incubation, the samples demonstrated safe disinfection for Escherichia coli. For these reasons, the use of these new bioactive glasses can be considered a promising alternative for the reconstruction of bone defects and even for the treatment and suppression of bone infections., This research was performed with support from the Brazilian government through the funding agencies CAPES (CsF/PDSE-Project No 88881.189953/2018–01), CNPq (GD-Project No 142172/2016–2, Universal Research Project No 481260/2012–9 and PD. 312135/2016–5) and FAPESP (Project No 1999/01924–2).

[Aim]: We investigated the antioxidant protective power of egg white hydrolysate (EWH) against the vascular damage induced by mercury chloride (HgCl2) exposure in resistance arteries., [Methods]: Male Wistar rats received for 60 days: (I) intramuscular injections (i.m.) of saline and tap water by gavage – Untreated group; (II) 4.6 μg/kg of HgCl2 i.m. for the first dose and subsequent doses of 0.07 μg/kg/day and tap water by gavage – HgCl2 group; (III) saline i.m. and 1 g/kg/day of EWH by gavage – EWH group, or (IV) the combination of the HgCl2 i.m. and EWH by gavage – EWH + HgCl2 group. Blood pressure (BP) was indirectly measured and dose-response curves to acetylcholine (ACh), sodium nitroprusside (SNP), and noradrenaline (NE) were assessed in mesenteric resistance arteries (MRA), as in situ production of superoxide anion, nitric oxide (NO) release, vascular reactive oxygen species (ROS), lipid peroxidation, and antioxidant status., [Results]: Egg white hydrolysate prevented the elevation in BP and the vascular dysfunction after HgCl2 exposure; restored the NO-mediated endothelial modulation and inhibited the oxidative stress and inflammatory pathways induced by HgCl2., [Conclusion]: Egg white hydrolysate seems to be a useful functional food to prevent HgCl2-induced vascular toxic effects in MRA., This work was supported by National Council for Scientific and Technological Development – CNPq [CNPq 307399/2017-6]; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Programa Nacional de Cooperação Acadêmica; Pró-reitoria de Pesquisa - Universidade Federal do Pampa [N° 20180615102630]; Fundação de Amparo à Pesquisa do Rio Grande do Sul – FAPERGS/Brazil [PQG:19/2551-0001810-0]; Fundação de Amparo à Pesquisa e Inovação do Espírito Santo – FAPES/CNPq/PRONEX [N° 80598773], and Spanish Goverment (MICINN) [AGL-2017-89213].

A combination of x-ray-diffraction, x-ray absorption, x-ray magnetic circular dichroism, macroscopic magnetization, and muon-spin relaxation measurements is used to investigate the interplay between structure (both crystallography and microstructure) and magnetism in Sr2YIrO6 as a function of both chemical (Ca-doping) and physical (hydrostatic) pressure. X-ray absorption spectroscopy clearly shows that the physical pressure is more effective in modifying the structure. On the other hand, the dichroic measurements evidence a constant magnetic signal with physical pressure and strong differences with Ca-doping. Muon-spin relaxation reveals the presence of magnetic order, even when this is hidden in the magnetization data. From the combined analysis, the magnetic results are explained in terms of the presence of Ir6+ or Ir4+ ions in magnetic clusters, most likely located at and triggered by antisite disorder. The measurements under high physical pressure indicate that the magnetic state is independent of the crystallographic details such as Ir-O distances and Ir-O-Y angles., This work was partially supported by the Spanish MINECO projects MAT2014-54425-R and MAT2017-83468-R. E.A.-E. acknowledges the Spanish MINECO and the European Social Fund for a FPI (Formación de Personal Investigador, 2015) grant. C.A.E.Jr. is supported by FAPESP (Brazil) under Contract No. 2016/24137-3. This research used resources of the APS, a US Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357.

Forest edge effects promote striking microclimatic shifts that result in greater light exposure, increased air temperature and evapotranspiration rates, and reduced soil moisture can alter stem hydraulic conductivity. These environmental conditions greatly increase the risk of emboli in xylem, which can disrupt or even cease water transport. We investigated tropical tree species in contrasting sites to understand the influence that edge effects have on wood functional anatomy. We compared wood anatomical traits between trees of forest edge and forest interior sites. The species exhibited two types of xylem arrangements based on the distribution of parenchyma cells: Pseudopiptadenia inaequalis and Copaifera lucens, with paratracheal axial parenchyma (PP), and Eugenia excelsa and Erythroxylum cuspidifolium, with predominant apotracheal axial parenchyma (AP). Tree species with PP xylem possessed shorter and wider vessel elements and lower ray and vessel frequencies, while species with AP xylem possessed longer and narrower vessel elements and higher ray and vessel frequencies. Regardless of the axial parenchyma arrangement, all four species had a greater percentage of axial parenchyma at the edge sites. The different xylem arrangements showed distinct responses to the edge effect in vessel frequency. The tropical tree species studied here shift to a greater abundance of axial parenchyma when subjected to effects of forest edge, probably to ensure safe water transport. This behavior, in the face of environmental stresses, is a plastic response, and likely due to functional versatility of parenchyma cells. The patterns of vessel frequency for PP and AP suggest that these xylem arrangements can be considered functional groups since the species in each group share similar traits and have similar strategies for conducting and maintaining water flow., This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); and Fundação de Amparo à Pesquisa do Rio de Janeiro (FAPERJ); Rede de Pesquisas PPBio Mata Atlântica – Brasil (PPBio-Ma).

During the later stages of seed maturation, two key adaptive traits are acquired that contribute to seed lifespan and dispersal, longevity and dormancy. The seed‐specific heat shock transcription factor A9 is an important hub gene in the transcriptional network of late seed maturation. Here, we demonstrate that HSFA9 plays a role in thermotolerance rather than in ex situ seed conservation. Storage of hsfa9 seeds of Medicago truncatula and Arabidopsis had comparable lifespan at moderate storage relative humidity (RH), whereas at high RH, hsfa9 seeds lost their viability much faster than wild type seeds. Furthermore, we show that in M. truncatula, Mthsfa9 seeds acquired more dormancy during late maturation than wild type. Transient expression of MtHSFA9 in hairy roots and transcriptome analysis of Mthsfa9 Tnt1 insertion mutants identified a deregulation of genes involved in ABA biosynthesis, catabolism and signalling. Consistent with these results, Mthsfa9 seeds exhibited increased ABA levels and higher sensitivity to ABA. These data suggest that in legumes, HSFA9 acts as a negative regulator of the depth of seed dormancy during seed development via the modulation of hormonal balance., This research was conducted in the framework of the regional programme ‘Objectif Végétal, Research, Education and Innovation in Pays de la Loire’, supported by the French Region Pays de la Loire, Angers Loire Métropole and the European Regional Development Fund. The hsfa9 mutants of Medicago truncatula used in this research project, which are jointly owned by the Centre National de la Recherche Scientifique, were obtained from the Samuel Roberts Noble Foundation, Inc. and were created through research funded, in part, by a grant from the National Science Foundation (NSF# 703285). DPB was financed by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001. EAAS was financed by FAPESP (process number 17/50211–9). SB was financed by the AgreenSkills+ fellowship program, which has received funding from the EU's Seventh Framework Program under grant agreement No. FP7‐609398.

The next generation of galaxy surveys will allow us to test one of the most fundamental assumptions of the standard cosmology, i.e. that gravity is governed by the general theory of relativity (GR). In this paper, we investigate the ability of the Javalambre Physics of the AcceleratingUniverseAstrophysical Survey (J-PAS) to constrainGR and its extensions. Based on the J-PAS information on clustering and gravitational lensing, we perform a Fisher matrix forecast on the effective Newton constant, mu, and the gravitational slip parameter, eta, whose deviations from unity would indicate a breakdown of GR. Similar analysis is also performed for the DESI and Euclid surveys and compared to J-PAS with two configurations providing different areas, namely an initial expectation with 4000 deg(2) and the future best case scenario with 8500 deg(2). We show that J-PAS will be able to measure the parameters mu and eta at a sensitivity of 2-7 per cent, and will provide the best constraints in the interval z = 0.3-0.6, thanks to the large number of ELGs detectable in that redshift range. We also discuss the constraining power of J-PAS for dark energy models with a time-dependent equation-of-state parameter of the type w(a) = w(0) + w(a)(1 - a), obtaining Delta w(0) = 0.058 and Delta w(a) = 0.24 for the absolute errors of the dark energy parameters.© 2020 The Author(s) Published by Oxford University Press on behalf of the Royal Astronomical Society, We are thankful to our colleagues of J-PAS Theory Working Group for useful discussions and to Ricardo Landim for his comments. MAR and ALM acknowledge support from MINECO (Spain) project FIS2016-78859-P (AEI/FEDER, UE), Red Consolider MultiDark FPA2017-90566-REDC, and UCM pre-doctoral grant. JSA acknowledges support from FAPERJ grant no. E-26/203.024/2017; CNPq grant no. 310790/2014-0 and 400471/2014-0; and the Financiadora de Estudos e Projetos -FINEP grants REF. 1217/13-01.13.0279.00 and REF0859/10-01.10.0663.00. SC acknowledges support from CNPq grant nos 307467/2017-1 and 420641/2018-1. This paper has gone through internal review by the J-PAS collaboration. Funding for the J-PAS Project has been provided by the Governments of Espana and Aragon through the Fondo de Inversion de Teruel, European FEDER funding, the MINECO projects AYA2015-66211-C2-1- P, AYA2015-66211-C2-2, and AYA2012-30789; the Brazilian agencies FINEP, FAPESP, FAPERJ and by the National Observatory of Brazil.

We compare the oxygen abundance (O/H) of the narrow-line regions (NLRs) of Seyfert 2 AGNs obtained through strong-line methods and from direct measurements of the electron temperature (Te-method). The aim of this study is to explore the effects of the use of distinct methods on the range of metallicity and on the mass-metallicity relation of active galactic nuclei (AGNs) at low redshifts (z, We thank the referee for helping to improve this paper with her/his constructive feedback. OLD and ACK thank FAPESP and CNPq for the financial support. Funding for the SDSS and SDSS-II has been provided by the Alfred P. Sloan Foundation, the Participating Institutions, the National Science Foundation, the U.S. Department of Energy, the National Aeronautics and Space Administration, the Japanese Monbukagakusho, the Max Planck Society, and the Higher Education Funding Council for England. The SDSS Web Site is http://www.sdss.org/. The SDSS is managed by the Astrophysical Research Consortium for the Participating Institutions. The Participating Institutions are the American Museum of Natural History, Astrophysical Institute Potsdam, University of Basel, University of Cambridge, Case Western Reserve University, University of Chicago, Drexel University, Fermilab, the Institute for Advanced Study, the Japan Participation Group, Johns Hopkins University, the Joint Institute for Nuclear Astrophysics, the Kavli Institute for Particle Astrophysics and Cosmology, the Korean Scientist Group, the Chinese Academy of Sciences (LAMOST), Los Alamos National Laboratory, the Max-Planck-Institute forAstronomy (MPIA), the Max-Planck-Institute for Astrophysics (MPA), New Mexico State University, Ohio State University, University of Pittsburgh, University of Portsmouth, Princeton University, the United States Naval Observatory, and the University of Washington. We also thank to Max-Planck-Institute forAstrophysics and John Hopkins University for Physical properties for galaxies andAGNs in the SDSS: Data catalogues from SDSS studies atMPA/JHU. This research hasmade use of the VizieR catalogue access tool, CDS, Strasbourg, France (doi: 10.26093/cds/vizier). The original description of the VizieR service was published in AAS, 143, 23. This work has also made use of the computing facilities of the Laboratory of Astroinformatics (IAG/USP, NAT/Unicsul), whose purchase was made possible by the Brazilian agency FAPESP (grant no. 2009/54006-4) and the INCT-A.

While it is widely accepted that planets are formed in protoplanetary disks, there is still much debate on when this process happens. In a few cases protoplanets have been directly imaged, but for the vast majority of systems, disk gaps and cavities—seen especially in dust continuum observations—have been the strongest evidence of recent or ongoing planet formation. We present Atacama Large Millimeter/submillimeter Array observations of a nearly edge-on (i = 75°) disk containing a giant gap seen in dust but not in 12CO gas. Inside the gap, the molecular gas has a warm (100 K) component coinciding in position with a tentative free–free emission excess observed with the Karl G. Jansky Very Large Array. Using 1D hydrodynamic models, we find the structure of the gap is consistent with being carved by a planet with 4–70 MJup. The coincidence of free–free emission inside the planet-carved gap points to the planet being very young and/or still accreting. In addition, the 12CO observations reveal low-velocity large-scale filaments aligned with the disk major axis and velocity coherent with the disk gas that we interpret as ongoing gas infall from the local interstellar medium. This system appears to be an interesting case where both a star (from the environment and the disk) and a planet (from the disk) are growing in tandem., F.O.A. and P.C. acknowledge financial support from the Max Planck Society. L.I.C. acknowledges support from the David and Lucille Packard Foundation and NASA ATP 80NSSC20K0529. J.M.G. is supported by the grant AYA2017-84390-C2-R (AEI/FEDER, UE). G.A.P.F. acknowledges support from CNPq and FAPEMIG (Brazil). A.Z. acknowledges support from the FONDECYT Iniciacion en investigacion project number 11190837.

We build detailed composite models of photoionization and shock ionization based on the suma code to reproduce emission lines emitted from the Narrow Line Regions (NLR) of Seyfert 2 nuclei. The aim of this work is to investigate diagram active galactic nucleus (AGN) positions according to shock parameters, shock effects on the gas temperature and ionization structures and derive a semi-empirical abundance calibration based on emission-line ratios little sensitive to the shock presence. The models were used to reproduce optical (3000 < λ(Å) < 7000) emission line intensities of 244 local ($z \: \lesssim \: 0.4$) Seyfert 2s, whose observational data were selected from Sloan Digital Sky Survey DR7. Our models suggest that shocks in Seyfert 2 nuclei have velocities in the range of 50-300 $\rm km \: s^{-1}$ and imply a narrower metallicity range ($0.6 \: \lesssim \: (Z/Z_{\odot }) \: \lesssim \: 1.6$) than those derived using pure photoionization models. Our results indicate that shock velocity in AGNs cannot be estimated using standard optical line ratio diagrams, based on integrated spectra. Our models predict a different temperature structure and $\rm O^{+}$/O and $\rm O^{2+}$/O fractional abundances throughout the NLR clouds than those derived from pure photoionization models, mainly in shock-dominated objects. This suggests that, in order to minimize the shock effects, the combination of emission-lines emitted by ions with similar intermediate ionization potential could be good metallicity indicators. Finally, we derive two calibrations between the N/O abundance ratio and the N2O2 = log([N ii]λ6584/[O ii]λ3727) and N2 = log([N ii]λ6584/H α) indexes which agree with that derived from pure photoionization models. © 2020 The Author(s). Published by Oxford University Press on behalf of Royal Astronomical Society, We are grateful to the referee for his/her dedicated work in reviewing our paper. OLD and ACK are grateful to Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) and Conselho Nacional de Desenvolvimento Cient ' ifico e Tecnologico (CNPq). RAR thanks partial financial support from Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul (17/2551-0001144-9 and 16/25510000251-7) and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (302280/2019-7). MVC and GFH are grateful to Consejo Nacional de Investigaciones Cient ' ificas y T ' ecnicas (CONICET)., With funding from the Spanish government through the Severo Ochoa Centre of Excellence accreditation SEV-2017-0709.

Management of discarded tires is a compelling environmental issue worldwide. Although there are several approaches developed to recycle waste tire rubbers, their application in solid-state cooling is still unexplored. Considering the high barocaloric potential verified for elastomers, the use of waste tire rubber (WTR) as a refrigerant in solid-state cooling devices is very promising. Herein, we investigated the barocaloric effects in WTR and polymer blends made of vulcanized natural rubber (VNR) and WTR, to evaluate its feasibility for solid-state cooling technologies. The adiabatic temperature changes and the isothermal entropy changes reach giant values, as well as the performance parameters, being comparable or even better than most barocaloric materials in literature. Moreover, pure WTR and WTR-based samples also present a faster thermal exchange than VNR, consisting of an additional advantage of using these discarded materials. Thus, the present findings evidence the encouraging perspectives of employing waste rubbers in solid-state cooling based on barocaloric effects, contributing to both the recycling of polymers and the sustainable energy technology field., The authors acknowledge financial support from FAPESP (No. 2012/03480-0), CNPq and CAPES. The authors also thank LNLS and CNPEM.

All authors: Ortiz, J. L.; Santos-Sanz, P.; Sicardy, B.; Benedetti-Rossi, G.; Duffard, R.; Morales, N.; Braga-Ribas, F.; Fernández-Valenzuela, E.; Nascimbeni, V.; Nardiello, D.; Carbognani, A.; Buzzi, L.; Aletti, A.; Bacci, P.; Maestripieri, M.; Mazzei, L.; Mikuz, H.; Skvarc, J.; Ciabattari, F.; Lavalade, F. Scarfi, G.; Mari, J. M.; Conjat, M.; Sposetti, S.; Bachini, M.; Succi, G.; Mancini, F.; Alighieri, M.; Dal Canto, E.; Masucci, M.; Vara-Lubiano, M.; Gutiérrez, P. J.; Desmars, J.; Lecacheux, J.; Vieira-Martins, R.; Camargo, J. I. B.; Assafin, M.; Colas, F.; Beisker, W.; Behrend, R.; Mueller, T. G.; Meza, E.; Gomes-Junior, A. R.; Roques, F.; Vachier, F.; Mottola, S.; Hellmich, S.; Campo Bagatin, A.; Alvarez-Candal, A.; Cikota, S.; Cikota, A.; Christille, J. M.; Pál, A.; Kiss, C.; Pribulla, T.; Komžík, R.; Madiedo, J. M.; Charmandaris, V.; Alikakos, J.; Szakáts, R.; Farkas-Takács, A.; Varga-Verebélyi, E.; Marton, G.; Marciniak, A.; Bartczak, P.; Butkiewicz-Baķ, M.; Dudziński, G.; Alí-Lagoa, V.; Gazeas, K.; Paschalis, N.; Tsamis, V.; Guirado, J. C.; Peris, V.; Iglesias-Marzoa, R.; Schnabel, C.; Manzano, F.; Navarro, A.; Perelló, C.; Vecchione, A.; Noschese, A.; Morrone, L., Context. Deriving physical properties of trans-Neptunian objects is important for the understanding of our Solar System. This requires observational efforts and the development of techniques suitable for these studies. Aims. Our aim is to characterize the large trans-Neptunian object (TNO) 2002 TC302. Methods. Stellar occultations offer unique opportunities to determine key physical properties of TNOs. On 28 January 2018, 2002 TC302 occulted a mv ∼ 15.3 star with designation 593-005847 in the UCAC4 stellar catalog, corresponding to Gaia source 130957813463146112. Twelve positive occultation chords were obtained from Italy, France, Slovenia, and Switzerland. Also, four negative detections were obtained near the north and south limbs. This represents the best observed stellar occultation by a TNO other than Pluto in terms of the number of chords published thus far. From the 12 chords, an accurate elliptical fit to the instantaneous projection of the body can be obtained that is compatible with the near misses. Results. The resulting ellipse has major and minor axes of 543 ± 18 km and 460 ± 11 km, respectively, with a position angle of 3 ± 1 degrees for the minor axis. This information, combined with rotational light curves obtained with the 1.5 m telescope at Sierra Nevada Observatory and the 1.23 m telescope at Calar Alto observatory, allows us to derive possible three-dimensional shapes and density estimations for the body based on hydrostatic equilibrium assumptions. The effective diameter in equivalent area is around 84 km smaller than the radiometrically derived diameter using thermal data from Herschel and Spitzer Space Telescopes. This might indicate the existence of an unresolved satellite of up to ∼300 km in diameter, which is required to account for all the thermal flux, although the occultation and thermal diameters are compatible within their error bars given the considerable uncertainty of the thermal results. The existence of a potential satellite also appears to be consistent with other ground-based data presented here. From the effective occultation diameter combined with absolute magnitude measurements we derive a geometric albedo of 0.147 ± 0.005, which would be somewhat smaller if 2002 TC302 has a satellite. The best occultation light curves do not show any signs of ring features or any signatures of a global atmosphere. © ESO 2020., This research was partially based on data taken at the Sierra Nevada Observatory, which is operated by the Instituto de Astrofisica de Andalucia (CSIC). This research is also partially based on data taken at the German-Spanish Calar Alto observatory, which is jointly operated by the Max Planck Institute fur Astronomie and the Instituto de Astrofisica de Andalucia (CSIC). Part of the results were also based on observations taken at the 1.6m telescope on Pico dos Dias Observatory. This research was partially based on observations collected at the Schmidt telescope 67/92 cm (Asiago, Italy) of the INAF - Osservatorio Astronomico di Padova. Funding from Spanish projects AYA2014-56637-C2-1-P, AYA2017-89637-R, from FEDER, and Proyecto de Excelencia de la Junta de Andalucia 2012-FQM1776 is acknowledged. We would like to acknowledge financial support by the Spanish grant AYA-RTI2018-098657-JI00 "LEO-SBNAF" (MCIU/AEI/FEDER, UE) and the financial support from the State Agency for Research of the Spanish MCIU through the "Center of Excellence Severo Ochoa" award for the Instituto de Astrofisica de Andalucia (SEV- 2017-0709). Part of the research received funding from the European Union's Horizon 2020 Research and Innovation Programme, under grant agreement no. 687378 and from the ERC programme under Grant Agreement no. 669416 Lucky Star. The following authors acknowledge the respective CNPq grants: FB-R 309578/2017-5; RV-M 304544/2017-5, 401903/2016-8; J.I.B.C. 308150/2016-3; MA 427700/2018-3, 310683/2017-3, 473002/2013-2. This study was financed in part by the CoordenacAo de Aperfeiacoamento de Pessoal de Nivel Superior - Brasil (CAPES) - Finance Code 001 and the National Institute of Science and Technology of the e-Universe project (INCT do e-Universo, CNPq grant 465376/2014-2). GBR acknowledges CAPES-FAPERJ/PAPDRJ grant E26/203.173/2016, MA FAPERJ grant E-26/111.488/2013 and ARGJr FAPESP grant 2018/11239-8. E.F.-V. acknowledges support from the 2017 Preeminent Postdoctoral Program (P3) at UCF. C.K., R.S., A.F-T., and G.M. have been supported by the K-125015 and GINOP-2.3.2-15-2016-00003 grants of the Hungarian National Research, Development and Innovation Office (NKFIH), Hungary. G.M. was also supported by the Hungarian National Research, Development and Innovation Office (NKFIH) grant PD-128 360. R.K. and T.P. were supported by the VEGA 2/0031/18 grant. We acknowledge the use of Occult software by D. Herald.