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The high quality of the various biotypes present in the natural or cultivated state is one of the main features for caper production. Up to now, however, no selection activity has been carried out in order to identify the most suitable accessions for providing better quality products. In this paper, we report the first results of a selection of caper genotypes characterised by significant qualitative traits. A micropropagation protocol was evaluated in order to improve and allow the multiplication of the most promising Capparis spinosa L. subsp. spinosa genotypes, selected in Salina (Aeolian Islands), Sicily (Italy), in collaboration with the most important local growers.

Since the mid-1990s, there has been a growing interest among consumers and producers in downscaling to a local level the length of the agri-food chains as a solution for fairer and more sustainable food production systems. From the point of view of consumption, the attribute “local” is assuming an important role in defining food purchasing preferences, both in terms of expectations of product quality and in terms of its perceived relevance in determining the supply chain sustainability. This research aims to define how individuals’ perception of local production influences the definition of “local” among consumers based on a survey submitted to a sample of 500 consumers in North-Western Italy. The paper provides: (i) a semantic map built on keywords adopted by the respondents to describe local production; (ii) a categorization of food consumers divided in clusters on the basis of their eating styles; and (iii) a characterization of consumers clusters according to the preferences and knowledge expressed towards local production. The results show that consumers’ awareness and attitudes towards the concept of the “local” are influenced by the joint effect of their socio-demographic profile and their food consumption style, with some unexpected evidences that would deserve to be deepen with further research. However, given this uncertainty, three main traits seem to characterize the consumers attitude towards the “local”: a positive relation among the dimensions of environment, local development and product quality and the strength of the link between local production and the reduction of the length of the supply chain. Ultimately, territoriality is perceived as an index of higher product quality (seasonal, therefore fresh and genuine).

In this study, almond skin of Prunus dulcis L. variety Casteltermini was characterised for its chemical composition and for the inhibitory activity towards food associated microorganisms belonging to pathogenic (Salmonella Enteritidis, Staphylococcus aureus, Escherichia coli and Listeria monocytgenes), spoilage (Pseudomonas poae and Brochotrix thermospacta) and useful (Levilactobacillus brevis and Saccharomyces cerevisiae) groups. The n-hexane extract of P. dulcis skin, investigated by GC–MS, evidenced linoleic, palmitic, and oleic acids as the main abundant compounds. Staphylococcus aureus ATCC 33862 growth was strongly limited by n-hexane extract and the minimum inhibition concentration (MIC) was 25.5 mg/mL. These results highlight the potential of almond by-products as food bio-preservatives as well as pharmaceutical products.

Lignocellulosic fibers and microcellulose have been obtained by simple alkaline treatment from softwood al- mond shells. In particular, the Prunus dulcis Miller (D.A.) Webb. was considered as a agro industrial waste largely available in southern Italy. The materials before and after purification have been characterized by 13C CPMAS NMR spectroscopy methodology. A proper data analysis provided the relative composition of lignin and holo- cellulose at each purification step and the results were compared with thermogravimetric analysis and FT-IR. To value the possibility of using this material in a circular economy framework, the fibrous cellulosic material was used to manufacture a handmade cardboard. The tensile performances on the prepared cardboard proved its suitability for packaging purposes as a sustainable material. These fibers along with the obtained microcellulose can represent a new use for the almond shells that are mainly used as firewood.

This paper focuses on the design of a robust Real Time Locating Systems (RTLS) based on the Ultra-Wide Band (UWB) technology for Augmented Reality (AR) applications in TV studios that require artists and/or a presenter to be accurately localized. According to a UWB-based measurement campaign, carried out in a TV studio environment, ranging measurements are heavily affected by the human body interference. Indeed, lots of outliers are present as the UWB receiver may synchronize to reflected paths, which result to be much stronger than the direct one. As a consequence, range errors are very large. In this context, to improve the localization performance, we increased the redundancy of the RTLS by employing more than one tag to localize the artists on the TV scene. In particular, we have applied the Extended Kalman Filter (EKF) algorithm to work with two and three tags. Moreover, an outlier detection and correction procedure have been defined and adopted for the ranging phase. The resulting localization performance, based on real range measurements, shows that the EKF with two tags outperforms by 83.5 % the one with single tag.

This study explores the processability, mechanical, and thermal properties of biocompostable composites based on poly (butylene adipate-co-terephthalate) (PBAT) as polymer matrix and microcrystalline cellulose (MCC) derived from softwood almond (Prunus dulcis) shells (as-MCC) as filler at two different weight concentration, i.e., 10 wt% and 20 wt%. The materials were processed by melt mixing and a commercial MCC (c-MCC) was used as filler comparison. The fibrillar shape of as-MCC particles was found to change the rheological behavior of PBAT, particularly at the highest concentration. The melt mixing processing allowed obtaining a uniform dispersion of both kinds of fillers, slightly reducing the L/D ratio of as-MCC fibers. The as-MCC particles led to a higher increase of the elastic modulus of PBAT if compared to the c-MCC counterparts. Both the MCC fillers caused a drastic reduction of the elongation at break, although it was higher than 120% also at the highest filler concentrations. DSC analysis revealed that both MCC fillers poorly affected the matrix crystallinity, although as-MCC induced a slight PBAT crystallinity increase from 8.8% up to 10.9% for PBAT/as-MCC 20%. Therefore, this work demonstrates the great potential of MCC particles derived from almond shells as filler for biocompostable composites fabrication.

The industrial production of citrus plants for ornamental use (ornacitrus) began in Italy at the end of the 1960s due to the need for many citrus nurseries to adapt their activities in a time of crisis for citriculture. Nowadays, the ornamental citrus nursery sector is a well-established industry in many European countries such as Portugal, Spain, Greece, and southern Italy. In Italy, nursery production of ornamental citrus plants has become prominent due to the gradual shutdown of many commercial citrus orchards. Currently, Italy maintains its leadership with more than 5.5 million ornacitrus plants produced annually. Ornamental citrus production regards mainly different cultivars of Citrus and Fortunella species, with lemon as the lead species. In this paper, the contribution of breeding and cultural techniques to the innovation of the sector is reported and discussed. This review aims to give an updated scientific and technical description of a sector with large competitive potential that remains still largely unexplored, pointing out its strengths and weaknesses.

Three caper (Capparis spinosa L.) biotypes grown on the Sicilian island of Salina (38&deg, 33&prime, 49&Prime, N) were micropropagated to evaluate two different in vitro culture systems: one using the traditional solid medium, and the other based on liquid culture in a PlantForm bioreactor. PlantForm is a temporary immersion system (TIS), a new propagation method in which the shoots undergo temporary immersion in a liquid medium in order to avoid the accumulation of gas through forced ventilation. This study proposes a protocol to improve the efficiency of in vitro propagation of caper plants, while also reducing production costs, because of the elimination of the gelling agent, and manual labor, requiring limited subcultures and posing minimal contamination risks. Our results show that the caper shoots propagated in bioreactors demonstrated good adaptability and better growth rates than those grown in the conventional system. Statistically significant differences were observed between plants grown in the PlantForm liquid culture and those grown in solid medium regarding the number and length of shoots, which were further promoted by the addition of plant growth regulators (PGRs). The relative growth and real proliferation rate of the caper explants were higher when using meta-Topolin than when using 6-benzylaminopurine as a PGR. Overall, the TIS improved in vitro caper culture by promoting the proliferation, length, and vigor of the shoots.

Previous research has suggested that activities such as community gardens could offer a wide range of health benefits. The aim of the article is to systematically review the available literature to analyse the magnitude of the phenomenon, the geographical distribution, and the main characteristics in terms of health outcomes and target populations. The search addresses the question whether the activity in community gardens improves health and well-being outcomes of individuals. From the total amount of 7226, 84 selected articles showed that:(1) up to 50% are published by U.S. universities or institutions; (2) up to 44% of the studies considered “community gardens” as the main activity of the research focus; (3) one-third of the studies included adults; (4) almost 25% of the studies used “general health” as the main outcome when investigating the benefits of community gardens; (5) the percentage of studies that achieved their outcomes was heterogeneous among the different health dimensions. In conclusion, while a certain degree of heterogeneity in the used definition and outcome still exist, community gardens may be a viable strategy for well-being promotion in terms of psychological, social, and physical health and may be considered as an innovative urban strategy to promote urban public health.

Almond is appreciated for its nutraceutical value and for the aromatic profile of the kernels. In this work, an almond collection composed of 96 Sicilian accessions complemented with 10 widely cultivated cultivars was phenotyped for the production of volatile organic compounds using a proton-transfer time-of-flight mass spectrometer and genotyped using the Illumina Infinium®18 K Peach SNP array. The profiling of the aroma was carried out on fresh and roasted kernels enabling the detection of 150 mass peaks. Sixty eight, for the most related with sulfur compounds, furan containing compounds, and aldehydes formed by Strecker degradation, significantly increased during roasting, while the concentration of fifty-four mass peaks, for the most belonging to alcohols and terpenes, significantly decreased. Four hundred and seventy-one robust SNPs were selected and employed for population genetic studies. Structure analysis detected three subpopulations with the Sicilian accessions characterized by a different genetic stratification compared to those collected in Apulia (South Italy) and the International cultivars. The linkage-disequilibrium (LD) decay across the genome was equal tor2 = 0.083. Furthermore, a high level of collinearity (r2 = 0.96) between almond and peach was registered confirming the high synteny between the two genomes. A preliminary application of a genome-wide association analysis allowed the detection of significant marker-trait associations for 31 fresh and 33 roasted almond mass peaks respectively. An accurate genetic and phenotypic characterization of novel germplasm can represent a valuable tool for the set-up of marker-assisted selection of novel cultivars with an enhanced aromatic profile.

Exciton band structures analysis provides a powerful tool to identify the exciton character of materials, from bulk to isolated systems, and goes beyond the mere analysis of the optical spectra. In this work, we focus on the exciton properties of molybdenum sisulfide (MoS2) by solving the ab initio many-body Bethe–Salpeter equation, as a function of momentum, to obtain the excitation spectra of both monolayer and bulk MoS2. We analyse the spectrum and the exciton dispersion on the basis of a model excitonic Hamiltonian capable of providing an efficient description of the excitations in the bulk crystal, starting from the knowledge of the excitons of a single layer. In this way, we obtain a general characterization of both bright and darks excitons in terms of the interplay between the electronic band dispersion (i.e. interlayer hopping) and the electron–hole exchange interaction. We identify for both the 2D and the 3D limiting cases the character of the lowest-energy excitons in MoS2, we explain the effects and relative weights of both band dispersion and electron–hole exchange interaction and finally we interpret the differences observed when changing the dimensionality of the system.

International audience; Dispersionless energy bands in k space are a peculiar property gathering increasing attention for the emergence of novel electronic, magnetic, and photonic properties. Here, we explore the impact of electronic flat bands on the light-matter interaction. The van der Waals interaction between the atomic layers of hexagonal boron nitride induces flat bands along specific lines of the Brillouin zone. The macroscopic degeneracy along these lines leads to van Hove singularities with divergent joint density of states, resulting in outstanding optical properties of the excitonic states. For the direct exciton, we report a giant oscillator strength with a longitudinal-transverse splitting of 420 meV, a record value, confirmed by our ab initio calculations. For the fundamental indirect exciton, flat bands result in phonon-assisted processes of exceptional efficiency, that compete with direct absorption in reflectivity, and that make the internal quantum efficiency close to values typical of direct band gap semiconductors.

The chromatin fiber folds into loops, but the mechanisms controlling loop extrusion are still poorly understood. Using super-resolution microscopy, we visualize that loops in intact nuclei are formed by a scaffold of cohesin complexes from which the DNA protrudes. RNA polymerase II decorates the top of the loops and is physically segregated from cohesin. Augmented looping upon increased loading of cohesin on chromosomes causes disruption of Lamin at the nuclear rim and chromatin blending, a homogeneous distribution of chromatin within the nucleus. Altering supercoiling via either transcription or topoisomerase inhibition counteracts chromatin blending, increases chromatin condensation, disrupts loop formation, and leads to altered cohesin distribution and mobility on chromatin. Overall, negative supercoiling generated by transcription is an important regulator of loop formation in vivo., e acknowledge support from European Union’s Horizon 2020 research and innovation program (CellViewer 686637 to J.S., M.L., and M.P.C.); Ministerio de Ciencia e Innovación (grant BFU2017-86760-P [AEI/FEDER, UE] to M.P.C.), and an AGAUR grant from Secretaria d’Universitats i Recerca del Departament d’Empresa i Coneixement de la Generalitat de Catalunya (2017 SGR 689 to M.P.C.); Centro de Excelencia Severo Ochoa (2013–2017 to M.P.C.); CERCA Programme/Generalitat de Catalunya (to M.P.C.); the Spanish Ministry of Science and Innovation to the European Molecular Biology Laboratory (EMBL) partnership (to M.P.C.); the National Natural Science Foundation of China (31971177 to M.P.C.); the Innovative Team Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory (2018GZR110103001 to M.P.C.); a Linda Pechenik Montague Investigator Award (to M.L.); a University of Pennsylvania Epigenetics Pilot Award (to M.L.); the Center for Engineering and Mechanobiology (CEMB) a National Science Foundation (NSF) Science and Technology Center Pilot Award (Division of Civil, Mechanical and Manufacturing Innovation [CMMI]: 15-48571 to M.L.); NIH grants R01HD091797 and R01GM123289 (to C.t.W.); the People Program (Marie Skłodowska-Curie Actions) FP7/2007–2013 under an REA grant (608959 to M.V.N.); Juan de la Cierva-Incorporación 2017 (to M.V.N.); Grant for the recruitment of early-stage research staff FI-2020 (Operational Program of Catalonia 2014–2020 CCI 2014ES05SFOP007 of the European Social Fund to L.M.); a “la Caixa” Foundation Fellowship (ID 100010434, LCF/BQ/DR20/11790016) (to L.M.); “La Caixa-Severo Ochoa” pre-doctoral fellowship (to Á.C.-G.); and Secretaria d’Universitats i Recerca del Departament d’Empresa i Coneixement de la Generalitat de Catalunya and the co-financing of Fondo Social Europeo (2018FI_B_00637 and FSE to R.S.-P.), and a La Caixa international PhD fellowship (to F.S.).

The genus Crepis L., included within the Asteraceae family, has a very wide distribution, expanding throughout the northern hemisphere, including Europe, northern Africa, and temperate Asia. This genus has a fundamental value from biodynamic and ecological perspectives, with the different species often being chosen for soil conservation, for environmental sustainability, and for their attraction towards pollinating species. Furthermore, various species of Crepis have been used in the popular medicine of several countries as medicinal herbs and food since ancient times. In most cases, the species is consumed either in the form of a decoction, or as a salad, and is used for its cardiovascular properties, as a digestive, for problems related to sight, for the treatment of diabetes, and for joint diseases. This literature review, the first one of the Crepis genus, includes publications with the word ‘Crepis’, and considers the single metabolites identified, characterised, and tested to evaluate their biological potential. The various isolated compounds, including in most cases sesquiterpenes and flavonoids, were obtained by extracting the roots and aerial parts of the different species. The secondary metabolites, extracted using traditional (solvent extraction, column chromatography, preparative thin layer chromatography, preparative HPLC, vacuum liquid chromatography), and modern systems such as ultrasounds, microwaves, etc., and characterised by mono- and bi- dimensional NMR experiments and by HPLC-MS, have a varied application spectrum at a biological level, with antimicrobial, antioxidant, antidiabetic, antitumor, antiviral, antiulcer, phytotoxic, and nutritional properties having been reported. Unfortunately, in vitro tests have not always been accompanied by in vivo tests, and this is the major critical aspect that emerges from the study of the scientific aspects related to this genus. Therefore, extensive investigations are necessary to evaluate the real capacity of the different species used in food, and above all to discover what the different plants that have never been analysed could offer at a scientific level.

The local fruit biodiversity can represent a good opportunity to maintain the agriculture and to develop economies in "marginal" territory such as those in the mountain rural areas. The genetic diversity of the Morus spp. is little known in the Western Pamir mountains, so that the objective of the present study was to improve the knowledge of the polymorphism of the species in the different poorly explored Tajik valleys by investigating the main quantitative and qualitative traits. Materials and methods - Six selected sites of the Gorno Badakhshan Autonomous Oblast (GBAO) region were investigated through field measurement on morphological traits of mulberry trees (both from wild and cultivated habitats). The qualitative analysis on fresh and dried fruits were carried out on four cultivars of the white mulberry (Morus alba L.) and one cultivar of the black mulberry (Morus nigra L.) collected in the Pamir Biological Institute, the highest botanical garden of the Commonwealth of Independent States (CIS). Results and discussion - Our study revealed high variability for all the measured parameters confirmed by a wide polymorphism. The highest rate of variability and the most promising genotypes in terms of fruit morphological traits were observed in the Vanch and the Rushan valleys. Due to the highest ascorbic acid content for the black cv. Shatut and the white cv. Marvori and a better taste, confirmed by the highest reducing and total sugar contents during the early and the full ripening (cv. Bedonia), mulberry fruits can be suggested for the fresh consumption. The cv. Shatut is the only one suggested for sun-drying, following specific local recipes for traditional consumption. Conclusion - The high morphogenetic variability of the mulberry trees in a marginal mountain area as the Gorno Badakhshan in the Western Pamir offers opportunities to make value of fruits with high nutritional and sensory profile and to develop the agricultural resources of any vulnerable territory.

A growing awareness that highly intensified agricultural systems have made a substantial worldwide contribution to the worsening of the resilience capacity of natural ecosystems has, over the last twenty years, brought general attention to agroecological management models. This aspect is even more evident in industrial agriculture, which is based on the use of multiple chemical products derived from non-natural synthesis. In more developed countries, a new idea of ecology linked to agricultural production has been increasingly developed and, for this reason, there has been a greater diffusion of differentiated agricultural models taking into consideration the environmental impact of production choices and policies addressed to the conservation of natural resources. In urban agricultural production, it is even more important to adopt resilient production models that, in addition to developing responsible production paths and allowing a positive connection with the needs of consumers, guarantees reasonable and positive behaviors respecting the environment in which most of the urban population lives; in other words, the implementation of goal 12 of the sustainable development goals (SDG #12 Responsible Production and Consumption) of the United Nations. In this work, we report some case studies inspired by the activities carried out by the Slow Food Association in Africa and demonstrate the importance of agroecological models in small-scale agricultural systems, related to the development of school and community gardens in small urban areas of different African countries, as a tool for integrating agricultural activities aimed at social resilience and the conservation of ecosystems.

Globally, almond production is experiencing a growing trend thanks to a strong interest in the health, gastronomic, and industrial properties that are linked to the fruits and their derivatives. After a constant and marked decline in the second half of the last century, the Mediterranean Basin is thoroughly reassessing this crop, which provides significant results with a modern orchard management. The opportunity determined by the transition from traditional to modern systems have increased the interest in evaluating the different environmental impacts of the two cultivation models that still coexist today. In this work, through the application of the Life Cycle Assessment (LCA) and an economic assessment approach with an in-depth analysis related to the cultivation cycle and the use of resources, the role played by each factor involved in production was determined. Overall, the Greenhouses Gases (GHG) emissions in modern farms are higher (Global Warming Potential (GWP) 0.224 kg CO2 eq.) than those in traditional farms (GWP 0.182 kg CO2 eq.). Regarding the economic assessment, it appears relevant that the modern almond model in the full production phase guarantees significantly higher margins (+84%).The perception of the importance attributed to evaluating economic and environmental aspects by different stakeholders shows relevant differences in the approach coming from growers, dealers, and governmental institutions allows the investigators to positively underline the current innovation in almond orchard systems considering the balance between fruit production and the conservation of environmental resources.

This research explored the preferences and buying habits of a sample of 620 consumers of fresh-cut, ready-to-eat salads. A best&ndash, worst scaling approach was used to measure the level of preference stated by individuals regarding 12 attributes for quality (intrinsic, extrinsic and credence) of fresh-cut salads. The experiment was carried out through direct interviews at several large-scale retail outlets in the Turin metropolitan area (north-west of Italy). Out of the total number of questioned consumers, 35% said they did not consume fresh-cut salads. On the contrary, the rest of the involved sample expressed the highest degree of preference towards the freshness/appearance attribute, followed by the expiration date and the brand. On the contrary, attributes such as price, organic certification and food safety did not emerge as discriminating factors in consumer choices. Additionally, five clusters of consumers were identified, whose preferences are related both to purchasing styles and socio-demographic variables. In conclusion, this research has highlighted the positive attitude of consumers towards quality products backed by a brand, providing ideas for companies to improve within this sector and implement strategies to answer the needs of a new segment of consumers, by determining market opportunities that aim to strengthen local brands.

Advances in cultivation of almonds: effects of genotypes, environment and cultural techniques

International audience; We perform a systematic study of the macroscopic dielectric function and electron energy loss (EEL) spectra for graphite. We obtain the dispersion behavior for the π plasmon, as a function of the momentum transfer q for two nonequivalent paths that traverse the first four Brillouin zones. We carry out these calculations within both time-dependent density functional theory (with two exchange-correlation functionals) and the Bethe-Salpeter equation. Additionally, we explore the effects of using the complete excitonic Hamiltonian (with all electron-hole pairs and antipairs), and within the Tamm-Dancoff approximation (neglecting antipairs). By analyzing the behavior of the macroscopic dielectric function, we are able to determine which peaks are predominantly from plasmonic behavior or only interband transitions. We compare the calculated spectra against several experiments that span almost five decades; our results present clear trends that follow the physical origins of the observed peaks. We carry out this study over a large range of momentum transfer in order to better evaluate the different theoretical methods compared to experiment, and predict the plasmonic behavior beyond available experimental data. Our results indicate that including the complete Hamiltonian with the exciton coupling included is essential for accurately describing the observed EEL spectra and plasmon dispersion of graphite, particularly for low values of momentum transfer. However, the solution of the Bethe-Salpeter equation is computationally intensive, so time-dependent density functional theory methods used in conjunction with the complete Hamiltonian may be an attractive alternative.

Rural development policies today include significant directions towards ecological transition and sustainability. Biodiversity plays a fundamental role, especially in fragile environments. The North African oases, for example, are socio-ecological structures with delicate balances in terms of natural resources, where the activation of participatory conservation approaches appears today to be very useful, aiming at long-lasting results. This type of approach was applied in the oasis of El Hamma, in Tunisia. The socio-ecological analysis was carried out through semi-structured interviews with different stakeholders of the oasis. The results were used to activate focus groups and to identify, in a participatory way, a conservation strategy for the species and the varieties at risk of erosion or disappearing. From this research, a wide spread of non-traditional date palm and vegetables emerged in a very diverse social context. These products were recognized as highly significant in terms of traditional knowledge by all stakeholders. Therefore, a Maison des semences and a public conservation center for perennial species were created, representing the first step of a participatory conservation model. Seeds of 11 traditional annual species, 10 date palm varieties and, in perspective, many other fruit species and vegetable varieties have been introduced into conservation.

Mouse embryonic stem cells (mESCs) are pluripotent and can differentiate into cells belonging to the three germ layers of the embryo. However, mESC pluripotency and genome stability can be compromised in prolonged in vitro culture conditions. Several factors control mESC pluripotency, including Wnt/β-catenin signaling pathway, which is essential for mESC differentiation and proliferation. Here we show that the activity of the Wnt/β-catenin signaling pathway safeguards normal DNA methylation of mESCs. The activity of the pathway is progressively silenced during passages in culture and this results into a loss of the DNA methylation at many imprinting control regions (ICRs), loss of recruitment of chromatin repressors, and activation of retrotransposons, resulting into impaired mESC differentiation. Accordingly, sustained Wnt/β-catenin signaling maintains normal ICR methylation and mESC homeostasis and is a key regulator of genome stability. We acknowledge the support from Spanish Ministry of Economy and Competitiveness and FEDER funds (SAF2011-28580, BFU2014-54717-P, BFU2017-86760-P and BFU2015-71984-ERC to M.P.C.), as well as ‘Centro de Excelencia Severo Ochoa 2013–2017’, Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement de la Generalitat de Catalunya grant (2014 SGR1137 to M.P.C.), AGAUR (SGR 2017-2019 to M.P.C) and the CERCA Programme/Generalitat de Catalunya (to M.P.C.), the European Union’s Horizon 2020 research and innovation programme under grant agreement CellViewer No 686637 (to M.P.C.), People Programme Marie Curie Actions of the European Union’s Seventh Framework Programme (FP7/2007–2013/, n° 290123 to I.T.), La Caixa international PhD fellowship (to F.S.) and Ministerio de Ciencia e Innovació FPI (to F.A.) and People Program (Marie Curie Actions) FP7/2007–2013 under REA grant (608959 to M.V.N.).

Millions of people worldwide suffer from visual disabilities as a result of retinal degeneration. Due to the poor regenerative capability of the central nervous system, retinal cell loss is essentially irreversible. Currently available therapies can only decelerate the degenerative process at late stages and are largely ineffective. However, the possibility of using stem cell-based therapy as cell rescue or cell replacement therapy is broadly being explored. While cell rescue is based on the secretion of biologically active molecules by the transplanted cells, cell replacement refers to the possibility of injected stem cells replacing the defective ones either via direct differentiation, transdifferentiation of the transplanted cells, or via cell fusion-mediated reprogramming of retinal cells.

A sustainable future for the community is one of the objectives established by the European Union Agenda 2030. Furthermore, sustainable consumption has been identified as one of the possible trajectories for sustainable development. It is for this reason that food production, distribution and consumption ways cannot be overlooked for sustainability achievement, as well as the consumer&rsquo, s related perception. In this research the Best&ndash, Worst scaling methodology was adopted to explore the priorities declared by a sample of 801 consumers among 12 different sustainability definitions selected from the scientific literature. The choice experiment was carried out through face-to-face interviews during two food and wine events closely related to the sustainability theme in the food sector. The respondents considered as sustainability priority definition the &ldquo, preservation of natural resources&rdquo, followed by &ldquo, decent working conditions&rdquo, and &ldquo, accessibility for everyone to healthy and safe food&rdquo, Moreover, 5 consumer&rsquo, s clusters were identified according to the priorities assigned to the different sustainability definitions, as well as to individuals socio-demographic characteristics. The description of the priorities assigned by the clusters to the different sustainability definitions have also been described as guidelines for consumer attitudes towards the different sustainability dimensions (environmental, social, economic and governance).

Gianni S., Sottile F. (2015): In vitro storage of plum germplasm by slow growth . Hort. Sci. (Prague), 42: 61–69. In this study, in vitro slow growth storage was investigated in four cultivars of two Sicilian (Southern Italy) plum species (Prunus domestica L. and Prunus cerasifera Ehrh. – two genotypes each). Established shoot cultures were preserved at 4°C in the dark in a Murashige and Skoog basal medium containing one of two different concentrations of sucrose (20 and 30 g/l) and with or without growth regulators. We tested the effects of cold storage, genotype and media on survival and re-growth capacity of explants after 3, 6, 9 and 12 months of storage. Effective minimal growth under cold conditions occurred in all four genotypes. The media composition did not affect survival, which, instead, appeared to be genotype-dependent. P. domestica genotypes survived cold storage the longest, for 12 and 9 months; instead, P. cerasifera ones remained viable for up to 6 months. All genotypes retained proliferation capacity under standard conditions and their re-growth capacity seems to be strongly genotype-dependent, closely related to their individual performance in response to the experimental condition of storage.

L'agroecologia viene spesso presentata come un'alternativa agli attuali sistemi di produzione diffusi, sebbene ci siano molte domande sulla reale possibilità di una produzione alimentare sufficiente- mente elevata per una popolazione in crescita. L' analisi sviluppata in questo paper è basata sulla ricer- ca scientifica degli ultimi vent'anni e sulle l'attività delle ONG, dei movimenti degli agricoltori e delle organizzazioni governative di diversi contesti. L'obiettivo è quello di fornire un panorama dei princi- pali argomenti in discussione per una transizione agroecologica dei sistemi alimentari mondiali, individ- uando alcune sfide prioritarie e immaginando chi saranno i partecipanti questo cambiamento. Data la complessità del tema, alcuni temi specifici non sono stati presi in considerazione (ad esempio valore nutrizionale degli alimenti, salute), invece ci siamo concentrati su quelle questioni trasversali che stanno infiammando il dibattito in contesti istituzionali e non istituzionali .

Imprinted genes control several cellular and metabolic processes in embryonic and adult tissues. In particular, paternally expressed gene-3 (Peg3) is active in the adult stem cell population and during muscle and neuronal lineage development. Here we have investigated the role of Peg3 in mouse embryonic stem cells (ESCs) and during the process of somatic cell reprogramming towards pluripotency. Our data show that Peg3 knockdown increases expression of pluripotency genes in ESCs and enhances reprogramming efficiency of both mouse embryonic fibroblasts and neural stem cells. Interestingly, we observed that altered activity of Peg3 correlates with major perturbations of mitochondrial gene expression and mitochondrial function, which drive metabolic changes during somatic cell reprogramming. Overall, our study shows that Peg3 is a regulator of pluripotent stem cells and somatic cell reprogramming. We thank Marie Victoire Neguembor, Martina Pesaresi and Sergi Angel Bonilla Pons for suggestions on the manuscript and Umberto Di Vicino for technical support. We thank Angelique di Domenico and the group of Antonella Consiglio for providing us the OPA1, DRP1 and VDAC1 antibodies. We thank Mari Carmen Ortells and the group of Bill Keyes for providing us the OXPHOS antibody. The pInducer10-mir-RUP-PheS plasmid was a gift from Stephen Elledge (Addgene plasmid # 44011). We are grateful for support from Ministerio de Economia y Competitividad and FEDER funds (SAF2011-28580, BFU2014-54717-P, and BFU2015-71984-ERC to M.P.C.), Secretaria d'Universitats i Recerca del Departament d'Economia i Coneixement de la Generalitat de Catalunya (2014 SGR1137 to M.P.C.), the European Union's Horizon 2020 research and innovation programme under grant agreement CellViewer No 686637 (to M.P.C.), Spanish Ministry of Economy and Competitiveness, 'Centro de Excelencia Severo Ochoa 2013-2017 and the CERCA Programme/Generalitat de Catalunya (to M.P.C.), People Programme Marie Curie Actions of the European Union's Seventh Framework Programme (FP7/2007-2013/, n° 290123 to I.T.), La Caixa international PhD fellowship (to F.S.), Ministerio de Ciencia e Innovació FPI (to F.A.) and Ministerio de Ciencia e Innovación FPI-Severo Ochoa (to A.C.G).

Combining nonresonant inelastic x-ray scattering experiments with state-of-the-art ab initio many-body calculations, we investigate the electronic screening mechanisms in strongly correlated CuO in a large range of energy and momentum transfers. The excellent agreement between theory and experiment, including the low-energy charge excitations, allows us to use the calculated dynamical screening as a safe building block for many-body perturbation theory and to elucidate the crucial role played by d-d excitations in renormalizing the band gap of CuO. In this way we can dissect the contributions of different excitations to the electronic self-energy which is illuminating concerning both the general theory and this prototypical material. Combining nonresonant inelastic x-ray scattering experiments with state-of-the-art ab initio many-body calculations, we investigate the electronic screening mechanisms in strongly correlated CuO in a large range of energy and momentum transfers. The excellent agreement between theory and experiment, including the low-energy charge excitations, allows us to use the calculated dynamical screening as a safe building block for many-body perturbation theory and to elucidate the crucial role played by d-d excitations in renormalizing the band gap of CuO. In this way we can dissect the contributions of different excitations to the electronic self-energy which is illuminating concerning both the general theory and this prototypical material. Combining nonresonant inelastic x-ray scattering experiments with state-of-the-art ab initio many-body calculations, we investigate the electronic screening mechanisms in strongly correlated CuO in a large range of energy and momentum transfers. The excellent agreement between theory and experiment, including the low-energy charge excitations, allows us to use the calculated dynamical screening as a safe building block for many-body perturbation theory and to elucidate the crucial role played by d-d excitations in renormalizing the band gap of CuO. In this way we can dissect the contributions of different excitations to the electronic self-energy which is illuminating concerning both the general theory and this prototypical material.

International audience; A promising route towards nanodevice applications relies on the association of graphene and transition metal dichalcogenides with hexagonal boron nitride (h-BN). Due to its insulating nature, h-BN has emerged as a natural substrate and gate dielectric for graphene-based electronic devices. However, some fundamental properties of bulk h-BN remain obscure. For example, the band structure and the position of the Fermi level have not been experimentally resolved. Here, we report a direct observation of parabolic dispersions of h-BN crystals using high-resolution angle-resolved photoemission spectroscopy (ARPES). We find that h-BN exfoliation on epitaxial graphene enables overcoming the technical difficulties of using ARPES with insulating materials. We show trigonal warping of the intensity maps at constant energy. The valence-band maxima are located around the K points, 2.5 eV below the Fermi level, thus confirming the residual p-type character of typical h-BN.

This study assesses consumer preferences during fruit and vegetable (FV) sales, considering the sociodemographic variables of individuals together with their choice of point of purchase. A choice experiment was conducted in two metropolitan areas in Northwest Italy. A total of 1170 consumers were interviewed at different FV purchase points (mass retail chains and open-air markets) using a paper questionnaire. The relative importance assigned by consumers to 12 fruit and vegetable product attributes, including both intrinsic and extrinsic quality cues, was assessed by using the best&ndash, worst scaling (BWS) methodology. The BWS results showed that &ldquo, origin&rdquo, &ldquo, seasonality&rdquo, and &ldquo, freshness&rdquo, were the most preferred attributes that Italian consumers took into account for purchases, while no importance was given to &ldquo, organic certification&rdquo, variety&rdquo, or &ldquo, brand&rdquo, Additionally, a latent class analysis was employed to divide the total sample into five different clusters of consumers, characterized by the same preferences related to FV attributes. Each group of individuals is described on the basis of sociodemographic variables and by the declared fruit and vegetable point of purchase. This research demonstrates that age, average annual income, and families with children are all discriminating factors that influence consumer preference and behavior, in addition to affecting which point of purchase the consumer prefers to acquire FV products from.

Italian germplasm is characterized by a wide diversity rapidly developed determining a massive genetic pool of cultivars in several growing areas. On the whole, regions of southern Italy, and in particular Sicily, were considered as one of the main trade routes along which almond was spread throughout the shores of the Mediterranean Sea. In this work, 9 SSR markers have been used to analyze 113 almond cultivars and accessions coming from ex-situ conservation, including most of almond genotypes spread in Sicily and Apulia and foreign cultivars from Mediterranean, American and Australian areas in order to determine the level of genetic diversity within Italian genotypes and elucidate phylogenetic and possible parentage relationships between Italian accessions and foreign germplasm. Distance and model-based analyses revealed a high level of variability both among and within geographical areas. In total, 159 alleles were detected altogether, which identified the average of 44 genotypes in the population investigated. The wide genetic diversity detected in the Italian almond cultivars represents a valuable source of variability to be applied for breeding. These data represent the first overview of genetic diversity among Italian almond cultivars using molecular markers.

We present a general picture of the exciton properties of layered materials in terms of the excitations of their single-layer building blocks. To this end, we derive a model excitonic hamiltonian by drawing an analogy with molecular crystals, which are other prototypical van der Waals materials. We employ this simplified model to analyse in detail the excitation spectrum of hexagonal boron nitride (hBN) that we have obtained from the {\it ab initio} solution of the many-body Bethe-Salpeter equation as a function of momentum. In this way we identify the character of the lowest-energy excitons in hBN, discuss the effects of the interlayer hopping and the electron-hole exchange interaction on the exciton dispersion, and illustrate the relation between exciton and plasmon excitations in layered materials., Accepted for Phys. Rev. B

Today, a large number of cost saving and energy efficient applications are enabled by Wireless Sensor and Actuator Networks (WSANs). Usually, these solutions have serious connectivity problems in scenarios where other wireless technologies are co-located sharing the frequency spectrum (e.g. industrial shop-floors). To cope with this issue, the concept of Multi-Radio (MR) has been introduced, which promotes the simultaneous use of multiple radio communication interfaces, leveraging their different characteristics, to improve the overall system performance and reliability. The proposed approach based on cognitive algorithm, considers two wireless technologies operating at the 2.4 GHz frequency band, namely Wi-Fi and 6LoWPAN, and provides a concrete implementation of the system using a real test-bed industry scenario. The solution provides a reliable communication infrastructure for manufacturing processes, firstly combining the properties of several physical layer standards and secondly, providing the ability to recover from temporary network failures by switching from a communication channel to another one.

Occupational safety and health (OSH) in industrial environments is gathering increasing attention in the era of Industry 4.0. In this context, location based services (LBS) can be adopted to support workers' safety in hazardous industrial environments. However, the provision of accurate location service in these harsh environments still faces big challenges. To address these challenges, this paper presents a robust hybrid localization algorithm that combines ultra-wideband (UWB) based ranging measurements and inertial measurement unit (IMU) data. The algorithm has been implemented on a proprietary wearable platform and its performance has been evaluated in an indoor environment. The experimental results show that the proposed hybrid algorithm outperforms a non-hybrid, UWB-based, Position --- Velocity (PV) extended Kalman filter (EKF), which has been chosen as benchmark, in terms of both location accuracy and availability. Thanks to a modular approach, the proposed solution also leads to a lower computation processing compared to other hybrid solutions.

A new technique to regenerate caper plants (Capparis spinosa L. subsp. rupestris) starting from flower explant is reported. In vitro plant regeneration was attempted using stigma, anthers and unfertilized ovules of unopened flowers collected in the field. Plant regeneration was achieved from unfertilized ovules on MS medium supplemented with 88 mM sucrose and 13 lM 6-benzyladenine (BA). New individuals obtained from unfertilized ovules were used as source material for micropropagation and multiple shoots were obtained on MS medium sup- plemented with the adeninic cytokinin BA and the auxin indole-3-butyric acid (IBA). Explants obtained in micro- propagation step were used for rooting step under several treatments. The best results (100% of rooted explants) were obtained when explants were dipped for 10 min in 50 lM IBA solution and successively maintained in growth regulator free medium. New plants were vigorous, of good quality and presented phenotypic characters similar to mother plants. Furthermore genetic stability of regenerants was verified through flow cytometric analysis and two different DNA-based techniques.

The Italian territory is rich in fruit trees germplasm and in the last years many research programs have been carried out to characterize local cultivars and accessions for deepenings about them and enhancing the market agreement too. The Sicilian plum cultivars 'Sanacore' and 'Ariddo di core' and the Piedmontese plum cultivar 'Ramassin' were studied to highlight their qualitative traits including the nutraceutical properties. Moreover, since it is important to know in which way the qualitative parameters change during the storage period one more study was carried out by storing the fruits under modified atmosphere in the Tectrol ® system. The results evidenced very interesting aspects about the qualitative characteristics of the cultivars studied and a positive influence of the modified atmosphere during storage.

The plum industry in Italy has shown substantial stability during the last ten years. About 14.000 hectares are currently grown using Japanese (75%) and European plum cultivars (25%). The most important destination is for fresh consumption, although a small production of dried fruits is still produced. The main growing areas are located in Emilia Romagna and Campania Regions where more than 50% of the total amount of the national commercial yield is gathered. At the recent Plum National Meeting, that was held in 2006 at Agrigento (Sicily) the main aspects related to breeding, cultivar assessment, rootstock trials, training systems and post-harvest management were pointed out. With this reference, this introductory lecture will briefly review these aspects, highlighting the most important traits of plum production in Italy as well as the development opportunities in new different areas where other stone fruits are traditionally grown.

An increase of plum industry has been evident in Italy since the 1990s. This growth was in both area planted and production and it has been especially evident in the South of Italy, where the environmental conditions favor the development of plum orchards, particularly those adopting Japanese cultivars. The Myrobalan seedling was the main rootstock historically used in the south of the country for plum orchards, on account of its strong vigor, hardiness, longevity and adaptability to a wide range of soil types. The introduction of new scion cultivars and new rootstocks has stimulated new studies on the main technical aspects related to plum cultivation, to evaluate the wide number of rootstock genotypes which are today available for the plum species. The Italian Ministry of Agriculture has sponsored a National project which aims to evaluate the most recent rootstocks licensed and available for European and Japanese plum cultivars. A series of experimental orchards have been established in different regions representative of the main Italian plum growing areas. This paper reports on the vegetative and qualitative traits observed both for European and Japanese plum cultivars, respectively, 'Stanley' and 'Shiro', grafted onto five rootstocks.

The Sicilian territory contains a wide reserve of biodiversity of fruit tree species that, over a period of several centuries, have found there propitious pedoclimatic conditions for their establishment, and genetic diversification. The indigenous germplasm characterized Sicilian agriculture until less than one century ago but, after the diffusion of new cultivars originating from genetic improvement programs, a great part of this germplasm was replaced and now these important resources are at high risk of genetic erosion. Recently, public interest in the safeguarding of biodiversity and the development of a new interest in genetic resources and their scientific, economic and cultural importance, inspired several political actions aimed at the valorisation and conservation of this material. The present study, funded by the Sicilian government, aims to improve knowledge concerning Sicilian plum germplasm, which accounts for a great number of the accessions on the Island, through an action of characterization that includes various steps: biometric, qualitative and nutraceutical. The study was preliminarily carried out on 26 accessions as a preliminary study to facilitate the development of an efficient methodology prior to extending it to other accessions localized in the Sicilian territory.

In this paper we propose a semi-random technique for the generation of a class of eIRA codes (a popular class of irregular LDPC codes that can be encoded in linear time) suited to partially parallel decoder implementations. The suggested technique tries to avoid both low-weight codewords and nearcodewords, which limit the performance of the belief propagation decoder. Its effectiveness is verified by comparison with literature results. The obtained codes are versatile, in terms of code-rate and block length, and they are characterized by a low error floor.

School gardens are, increasingly, an integral part of projects aiming to promote nutritional education and environmental sustainability in many countries throughout the world. In the late 1950s, FAO (Food and Agriculture Organization) and UNICEF (United Nations Children's Fund) had already developed projects to improve the dietary intake and behavior through school and community gardens. However, notwithstanding decades of experience, real proof of how these programs contribute to improving sustainability has not been well-documented, and reported findings have mostly been anecdotal. Therefore, it is important to begin a process of collecting and monitoring data to quantify the results and possibly improve their efficiency. This study’s primary goal is to propose an interpretive structure—the “Sustainable Agri-Food Evaluation Methodology-Garden” (SAEMETH-G), that is able to quantifiably guide the sustainability evaluation of various school garden organizational forms. As a case study, the methodology was applied to 15 school gardens located in three regions of Kenya, Africa. This application of SAEMETH-G as an assessment tool based on user-friendly indicators demonstrates that it is possible to carry out sustainability evaluations of school gardens through a participatory and interdisciplinary approach. Thus, the hypothesis that the original SAEMETH operative framework could be tested in gardens has also been confirmed. SAEMETH-G is a promising tool that has the potential to help us understand school gardens’ sustainability better and to use that knowledge in their further development all over the world.

International audience; We present the valence electron energy-loss spectrum and the dielectric function of monoclinic hafnia (m-HfO$_2$) obtained from time-dependent density-functional theory (TDDFT) predictions and compared to energy-filtered spectroscopic imaging measurements in a high-resolution transmission-electron microscope. Fermi's Golden Rule density-functional theory (DFT) calculations can capture the qualitative features of the energy-loss spectrum, but we find that TDDFT, which accounts for local-field effects, provides nearly quantitative agreement with experiment. Using the DFT density of states and TDDFT dielectric functions, we characterize the excitations that result in the m-HfO$_2$ energy loss spectrum. The sole plasmon occurs between 13-16 eV, although the peaks $\sim$28 and above 40 eV are also due to collective excitations. We furthermore elaborate on the first-principles techniques used, their accuracy, and remaining discrepancies among spectra. More specifically, we assess the influence of Hf semicore electrons (5$p$ and 4$f$) on the energy-loss spectrum, and find that the inclusion of transitions from the 4$f$ band damps the energy-loss intensity in the region above 13 eV. We study the impact of many-body effects in a DFT framework using the adiabatic local-density approximation (ALDA) exchange-correlation kernel, as well as from a many-body perspective using a $GW$-derived electronic structure to account for self-energy corrections. These results demonstrate some cancellation of errors between self-energy and excitonic effects, even for excitations from the Hf $4f$ shell. We also simulate the dispersion with increasing momentum transfer for plasmon and collective excitation peaks.

International audience; Low-dimensional materials differ from their bulk counterpart in many respects. In particular, thescreening of the Coulomb interaction is strongly reduced, which can have important consequencessuch as the significant increase of exciton binding energies. In bulk materials the binding energyis used as an indicator in optical spectra to distinguish different kinds of excitons, but this is notpossible in low-dimensional materials, where the binding energy is large and comparable in size forexcitons of very different localization. Here we demonstrate that the exciton band structure, whichcan be accessed experimentally, instead provides a powerful way to identify the exciton character.By comparing the ab initio solution of the many-body Bethe-Salpeter equation for graphane andsingle-layer hexagonal BN, we draw a general picture of the exciton dispersion in two-dimensionalmaterials, highlighting the different role played by the exchange electron-hole interaction and by theelectronic band structure. Our interpretation is substantiated by a prediction for phosphorene.

International audience; One of the big challenges of theoretical condensed-matter physics is the description, understanding, and prediction of the effects of the Coulomb interaction on materials properties. In electronic spectra, the Coulomb interaction causes a renormalization of energies and change of spectral weight. Most importantly, it can lead to new structures, often called satellites. These can be linked to the coupling of excitations, also termed dynamical effects. State-of-the-art methods in the framework of many-body perturbation theory, in particular, the widely used GW approximation, often fail to describe satellite spectra. Instead, approaches based on a picture of electron-boson coupling such as the cumulant expansion are promising for the description of plasmon satellites. In this work, we give a unified derivation of the GW approximation and the cumulant expansion for the one-body Green’s function. Using the example of bulk sodium, we compare the resulting spectral functions both in the valence and in the core region, and we discuss the dispersion of quasi-particles and satellites. We show that self-consistency is crucial to obtain meaningful results, in particular, at large binding energies. Very good agreement with experiment is obtained when the intrinsic spectral function is corrected for extrinsic and interference effects. Finally, we sketch how one can approach the problem in the case of the two-body Green’s function, and we discuss the cancellation of various dynamical effects that occur in that case.