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This paper uses autoethnography to reassess the concept "experience" and the lack of theoretical frameworks within experiential education for delimiting experience within the practices and research around experiential, adventure, and outdoor education. Although a pivotal and essential part of practice, theoretical understandings of experience have been missing in experiential education scholarship. Experience is clearly a complex, constructed "reality." Jagger (cited in Lauritzen, 1997, p. 83) has pointed out that an appeal to experience is "fraught with methodological difficulties." What exactly is experience? Whose experience is heard? Like other disciplines, for example the studies of religions and psychology, experiential education has no rigorous definitions, characterizations, typologies, or conceptualizations of the focus of its study and practice--a type of experience. Drawing upon critiques from Indigenous, feminist, postcolonial, and black Americans and Canadians, and integrating with an autoethnographic approach, this paper provides a critique of the existing use of "experience" and sketches an initial approach for developing theoretical understandings of the central phenomenon of experiential, adventure, and outdoor education.

Illustrates the subtlety of racism in daily life and suggests the responsibility of white people to acknowledge their access to privilege and power based on skin color, to remain accountable for the history of oppression of people of color, to change their attitudes and behaviors, and to work toward justice for all. (KS)

INTRODUCTION We shall discuss here the potential of proteomics (which defines the amino acid sequence of all proteins expressed by a specific cell type under specific growth conditions) and biomarker [...]

Anthrax is a highly fatal disease caused by the gram-positive, endospore-forming, rod-shaped bacterium Bacillus anthracis. Spores, rather than vegetative bacterial cells, are the source of anthrax infections. Spores of B. anthracis are enclosed by a prominent loose-fitting structure called the exosporium. The exosporium is composed of a basal layer and an external hair-like nap. Filaments of the hair-like nap are made up largely of a single collagen-like glycoprotein called BclA. A second glycoprotein, BclB, has been identified in the exosporium layer. The specific location of this glycoprotein within the exosporium layer and its role in the biology of the spore are unknown. We created a mutant strain of B. anthracis [DELTA] Sterne that carries a deletion of the bclB gene. The mutant was found to possess structural defects in the exosporium layer of the spore (visualized by electron microscopy, immunofluorescence, and flow cytometry) resulting in an exosporium that is more fragile than that of a wild-type spore and is easily lost. Immunofluorescence studies also indicated that the mutant strain produced spores with increased levels of the BclA glycoprotein accessible to the antibodies on the surface. The resistance properties of the mutant spores were unchanged from those of the wild-type spores. A bclB mutation did not affect spore germination or kinetics of spore survival within macrophages. BcLB plays a key role in the formation and maintenance of the exosporium structure in B. anthracis.

Certain carbohydrates (rhamnose, 3-O-methyl rhamnose, and galactosamine) have been demonstrated to be present in Bacillus anthracis spores but absent in vegetative cells. Others have demonstrated that these spore-specific sugars are constituents of the glycoprotein BclA. In the current work, spore extracts were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A second collagen-like glycoprotein, BclB, was identified in B. anthracis. The protein moiety of this glycoprotein was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) and the carbohydrate components by gas chromatography-mass spectrometry and tandem mass spectrometry. Spore-specific sugars were also demonstrated to be components of BclB.

This study examines the life history of Margaret Fleming (1901-1999) -- a Canadian mountaineer, editor, school teacher and single woman who lived in Winnipeg, Manitoba. Fleming, a lifetime member of the Alpine Club of Canada (ACC), was introduced to her first mountaineering camp in 1929 by Elizabeth Parker. Through the ACC, Fleming climbed in the Rockies, participated in early ski-mountaineering explorations into the Yoho National Park backcountry, served as the first woman editor of the Canadian Alpine Journal (1942-1952) and cultivated long-lasting friendships with other climbers. As a professional woman who juggled the responsibilities of work, family and leisure, Fleming's mountaineering life was shaped by the gendering of physical and social culture. Fleming integrated her pursuits as a genteel mountain woman and literary editor, within the context of an ongoing process of negotiations over women's place in mountain leisure.

ESI (electrospray ionization) MS and tandem mass spectrometry (MS/MS) were used for the analysis of single nucleotide polymorphisms (SNPs) and more complex genetic variations. Double-stranded (ds) PCR products were studied. PCR products of the proline [5'-(mathematical expression not reproducible in ASCII) (G(sub 17))- (mathematical expression not reproducible in ASCII) (C(sub 38)) (mathematical expression not reproducible in ASCII)-3'] and arginine variants [(5'-(mathematical expression not reproducible in ASCII)(G(sub 17))- (mathematical expression not reproducible in ASCII) (G(sub 38)) (mathematical expression not reproducible in ASCII)-3'] of the p53 gene are distinguished by an SNP (cytosine or guanine) and were discriminated using both quadrupole and quadrupole ion trap MS analysis. A 69 bp arginine mutant PCR product [5'-(mathematical expression not reproducible in ASCII)(C(sub 17))- (mathematical expression not reproducible in ASCII)(G(sub 38)) (mathematical expression not reproducible in ASCII)-3'] with a negating switch has the same mass as the proline variant but was readily distinguishable on ion trap MS/MS analysis; fragments containing the mutation site, but not the polymorphism, were identified. The 69 bp PCR products were restriction-enzyme-digested, to create 43 bp fragments. ESI quadrupole ion trap MS/MS analysis of the 43 bp product-ion spectra readily demonstrated both polymorphism and negating switch sites. MS and MS/MS are powerful and complemenmor techniques for analysis of DNA. MS can readily distinguish SNPs but MS/MS is required to differentiate isomeric PCR products (same nucleotide composition but different sequence).