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Okra plant is becoming to be one of favortite vegetable in Indonesia since then the deman of this plant is growing very rapidly. The aim of this research is to Utilize Palm Oil Liquid Organic Fertilizer and M-Bio Biofertilizer Against Growth and Production in Okra Plants (Abelmoschusesculentus L. Moench). This study used a Randomized Block Design (RBD) with 2 replications. Factorial consisting of II treatment factors, Factor I treatment given P0: Without Liquid Palm Oil Organic Fertilizer, P1: Provision of PKS liquid fertilizer and Factor II, treatment of giving M-Bio. Parameters observed in this study were vegetative dan generative. The results showed that the treatment of oil palm liquid organic fertilizer had no significant effect on vegetative and generative observed parameters. The treatment of M-Bio biofertilizer significantly affected the vegetative and generative observation parameters where the best dose was treatment 6%. The treatment of the combination of liquid organic fertilizer from palm oil waste and M-Bio biological fertilizer has no significant effect on all parameters of vegetative and generative observations.

The study aimed to determine the response of growth and production of peanuts to the provision of goat manure and banana hump liquid organic fertilizer. This study used a Randomized Block Design (RBD) with 3 replications. Factorial consisting of II treatment factors, Factor I treatment of giving goat manure and Factor II, the treatment of Hump Banana POC treatment The parameters observed in this study; Plant height (cm), number of branches (branches), number of pods per sample (fruit), wet weight of production per sample (g), wet weight of production per plot (g), production dry weight per sample (g), and dry weight Production Per Plot (g). The results showed that goat manure had an effect on growth but did not affect the production of peanut plants, liquid organic fertilizer of banana humps had an influence on the growth and production of peanut plants, Combination of goat manure and banana hump liquid organic fertilizer had no significant effect on growth and production of peanut plants.

In the field of food, coffee waste begins to be developed by processing it as a beverage product. The skin of coffee fruit can also be processed as a food product, one of which is jelly candy. This study aims to study jelly candy making with the addition of gelatin as a gelling agent and the addition of lemon juice to produce coffee fruit skin jelly candy that has physicochemical and sensory characteristics that meet SNI quality requirements. The design used was factorial Completely Randomized Design consisting of 2 (two) factors. Factor I concentration of gelatin (G) and Factor II is the concentration of lemon water (L). Factor I consists of 4 levels, namely G1 = 10%, G2 = 15%, G3 = 20% and G4 = 25%, while factor II consists of 3 levels, namely L1 = 5%, L2 = 10% and L3 = 15%. Each treatment was repeated 2 times to obtain 24 experimental units.The concentration of gelatin and the concentration of lemon have a very significant effect on water content and pH value. The concentration of lemon has a significant effect on the value of hedonic organoleptic taste. Both interactions have a very significant effect on the value of organoleptic hedonic texture. The results of the analysis of coffee fruit peel jelly candy have an average moisture content of 21.25%, 0.95% ash content, pH 4.53, sensory hedonic color (3.50 neutral reception), aroma (3.48 neutral acceptance), taste (3.50 neutral acceptance), texture (3.59 likes reception).The results showed that 10% gelatin concentration and 10% lemon concentration (G1L2) were the best treatments obtained through the ranking test. The jelly candy produced has 21.32% moisture content characteristics, 1.55% ash content, pH 4.71, 14.09% antioxidant activity, 3.03 mg / 100g vitamin C levels, 3.55 sensory colors (likes) , aroma 3.45 (neutral), taste 3.48 (neutral) and texture 3.43 (neutral).

This study aims to determine the best Picloram and BAP concentrations in InVitro patchouli callus formation. This experiment was conducted in November 2018 to January 2019 at the Tissue Culture Laboratory, Faculty of Agriculture, Andalas University, Padang. This study used an experimental method with Completely Randomized Design (CRD) consisting of two factors. The first factor was the administration of Picloram concentration with 5 levels of treatment and the second factor was the administration of BAP concentrations with 5 levels of treatment. This experiment consisted of: This experiment consisted of Factor I: Picloram (A) Without Picloram (A1), Concentration of 1 mg / L (A2), Concentration of 2mg / L (A3), Concentration of 3 mg / L (A4), Concentration of 4mg / L (A5), Factor II: Concentration of BAP (S) Without BAP (S1), Concentration of 0.5 mg / L (S2), Concentration of 1 mg / L (S3), Concentration of 1.5 mg / L (S4) Concentration 2 mg / L (S5). The data obtained were analyzed using the F test at the level of 5%, if F count is greater than F table then the analysis is continued with the DMNRT test at the level of 5%. From the results of the study showed that the concentration of picloram had a significant effect on the growth of patchouli callus with an average callus appearance of 7 HST and percentage (%) of explants growing up to 100%. High picloram concentration did not significantly affect callus growth, 2 mg / l picloram + BAP concentration did not produce callus.

Halalness of a food product is an important factor as a consideration in consuming it. Raw materials and additives used will affect the halality of the product. The food that we called to be halal if it does not contain pork including lard. This study was applied of the forensic microbiological analysis in assessing the total microbes contained in sausages containing lard. The aim of the study was to analyze the total microbes in beef sausages mixed with lard. In this total microbial analysis, a factorial Completely Randomized Design (RAL) method was used. Factor I is Solvent Concentration (K) which consists of four levels (K 1 = 20%, K 2 = 30%, K 3 = 40%, K 4 = 50%) and Factor II is Maseration Time (W) which consists of four levels (W 1 = 6 Hours, W 2 = 12 Hours, W 3 = 18 Hours, W 4 = 24 Hours). The results showed that the n-hexane concentration in beef sausage samples : lard (1: 1) gave a very significant effect (P> 0.01). The highest number of microbes was found in K 1 (9487,500) and the lowest was in K 4 (3887,500). The maceration time had a different effect that was not significant (P

The following fictional case is intended as a learning tool within the Pathology Competencies for Medical Education (PCME), a set of national standards for teaching pathology. These are divided into three basic competencies: Disease Mechanisms and Processes, Organ System Pathology, and Diagnostic Medicine and Therapeutic Pathology. For additional information, and a full list of learning objectives for all three competencies, see http://journals.sagepub.com/doi/10.1177/2374289517715040 . 1

Factor V Leiden (FVL) and factor II c.*97G > A mutation are the two most common genetic factors predisposing to hereditary thrombophilia. Being extremely rare, it used to be thought that double homozygosity for both variants is inconsistent with life. Only two cases describing double-homozygous patients with venous thrombosis were reported about two decades ago. However, to the best of our knowledge, there has been no reported case of double-homozygous individuals presenting with recurrent fetal losses rather than venous thrombosis. Herein, we present the case of a 36-year-old female who presented with recurrent first trimester miscarriages without developing venous thromboembolism. Testing with allele-specific polymerase chain reaction showed double-homozygous pattern for both FVL and factor II c.*97G > A mutation, both of which were confirmed by next generation DNA sequencing. With a population prevalence of less than one per ten million individuals, double-homozygotes’ actual increase in risk for venous thromboembolism or fetal loss is unknown.

Made available in DSpace on 2022-04-28T19:29:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-01 Objective The aim of this study is to evaluate the color alteration and shore A hardness of a medical silicone with extrinsic pigmentation, before and after accelerated aging. Materials and Methods Twenty samples (Silastic Q7-4735) were made with an intrinsic pigmentation. This intrinsic pigmentation was composed of a pink pigment (H-109-P, Factor II) and an opacifier (ZnO). All samples had standardized dimensions (45-mm diameter and 2-mm thickness). Half of the 20 samples manufactured subsequently received an extrinsic pigment (Tan FE-215, Factor II). Therefore, two groups were created (n = 10): Group 1, group with intrinsic pigmentation and without extrinsic pigmentation (control) and Group 2, group with intrinsic and extrinsic pigmentation. Samples were submitted to color and Shore A hardness tests, before and after 1,008 hours of aging. Statistical Analysis Color alteration data were submitted to Student's t -test (a = 0.05). Shore A hardness data were submitted to two-way analysis of variance and Tukey test (a = 0.05). Results The incorporation of the extrinsic pigment on the silicone did not affect its color (?E) when the two groups were compared (p = 0.232). Regarding the hardness test, the interaction between group and period did not interfere with the hardness results(p =0.599). However, the period factor showed that there was a reduction in the hardness of the silicone after aging (p < 0.05). Conclusion In this study, all the hardness and color results of the silicone used were clinically acceptable, regardless of the presence of extrinsic pigmentation. Department of Dental Materials and Prosthodontics São Paulo State University School of Dentistry Department of Pediatric and Social Dentistry São Paulo State University School of Dentistry Department of Dental Materials and Prosthodontics São Paulo State University School of Dentistry Department of Pediatric and Social Dentistry São Paulo State University School of Dentistry

There remains an unmet need for better anticoagulants. The phase I clinical trial is of great significance in the development of anticoagulants, and the design is special. This system review aims to provide insights for the design of future phase I clinical trials of anticoagulants. We searched the database PubMed and ClinicalTrail.gov website, to collate the phase I clinical trial of anticoagulants in healthy people. The study protocol, inclusion-exclusion criteria, safety, and pharmacodynamic indexes were reviewed. New anticoagulants under development focused on inhibiting one or more than one serine proteases within the coagulation cascade. Agents targeting intrinsic factors are in the pipeline of the drug development. The enrollment eligibility criteria have more restrictions on laboratory tests, medical history, or medication history related to bleeding and coagulation; more precautions were taken to assess and minimize the risk of hemorrhagic events. Pharmacodynamics markers were evaluated as a surrogate marker of anticoagulation potency to guide further dose selection in drug���s development. In future, the positive control study can be applied in phase I studies of new anticoagulants with appropriate pharmacodynamics markers, which can provide more favorable information on making ���go/no��� decision in drug development.

Laboratory testing for thrombophilia is complicated but essential for diagnosis. In 2017, the cobas® Factor II and Factor V Test (cobas F2F5 test) was launched for use with the cobas z 480 analyzer. This qualitative polymerase chain reaction test enables multiplex Factor II and Factor V testing with flexible reporting and workflow efficiency. Here, we report the results from studies investigating the performance of the cobas F2F5 test. Technical performance verification, clinical validation, external laboratory performance, and workflow comparison studies were performed. Fresh and frozen whole-blood and genomic DNA (gDNA) samples were tested, and several manual and automated DNA isolation methods were used. Bidirectional Sanger sequencing was used to verify genotypes identified by the cobas F2F5 test. One hundred percent agreement between the cobas F2F5 test and Sanger sequencing was observed for all genotypes. An external laboratory using remnant clinical samples also yielded 100% agreement between cobas F2F5 test results and their routine testing method. The cobas F2F5 test reduced the total sample processing time compared with the LightCycler® 1.2 platform (98.6 vs 420.2 min; 96 samples). Hemoglobin, extraction buffer, and ethanol contamination of the gDNA sample can lead to invalid results. The cobas F2F5 test has a high degree of accuracy for identification of Factor II and Factor V genotypes. This multiplex testing with short sample processing time can reduce handling errors and increase efficiency. Both manual and automated DNA isolation methods can be used with the cobas F2F5 test. Electronic supplementary material The online version of this article (10.1007/s11239-018-1745-8) contains supplementary material, which is available to authorized users.

The study examines secondary school students' perception of the climate in their classrooms with reference to inclusive education. A key objective of the study was also to examine psychometric properties of a newly developed Inclusion Climate Scale (ICS). Data were obtained using a paper-pencil survey in which 699 students (age = 10–17 years) from North Rhine-Westphalia (Germany) participated. The results of exploratory factor analysis revealed two factors (Factor I: Teacher Support and Care; Factor II: Emotional Experience). The Inclusion Climate Scale along with the two factors it contains showed satisfactory reliability and validity.

Aim Despite its high prevalence and seriousness, delirium has been underrecognized by nurses. One reason is that the original characteristics of delirium are relatively unspecific and phenomenologically diverse, which makes a nurse's subjective decision more important in delirium detection. This study aims to identify the experiences, practices, and viewpoints of nurses in recognizing delirium in the elderly. Methods In this study, Q-methodology, which is a method for analyzing subjectivity, was used. Following the steps of Q-methodology, 32 nurses with experiences in caring for the delirious elderly sorted 34 Q-statements into the shape of a normal distribution. A centroid factor analysis and varimax rotation using the PQMethod program were conducted. Results This study revealed four factors regarding nurses toward delirium recognition in the elderly. They were: Factor I, prediction from the integrated signs; Factor II, visible symptom-centered detection; Factor III, the detection of abnormal changes based on concentrated observation; and Factor IV, identification by relying on the diagnostic data. Conclusion The result of the study can help to understand elderly delirium detection more practically from a nurse's point of view. It is expected to be used as a basis for a practical and accessible delirium education for nurses that reflects nurses' subjective viewpoints.

Avian influenza is a kind of contagious disease which can spread not only to other poultry but also to the human (zoonosis). Avian influeza becomes world issue when the human can effect the death. Sarang Semut plant ( Myrmecodia . sp) which has much anti-oxidant and imunostimulant can increase immunity. Imunostimulant will help and protect body cells well. The increasing of cellular immunity will help the cell attack the AI virus and increase the work of avian influenza vaccine. Therefore, this research is done to know the influence of sarang semut extract toward poultry’s anti-body of avian influenza H5N1. This research using experimental method. The design used is RAL factorial. Factor I: the addition of sarang semut extract amounted 5mgs/kgs BB, 10 mg/kg BB, 15 mg/kg BB and Factor II: the length of giving sarang semut extract, namely: 3 days, 5 days, and 7 days. The result of this research was analyzed using variance analysis (ANOVA) followed by BNT. The result of serology examination shows that in adding sarang semut extract showing the significant different in each experiment, but not showing the significant different in the time given. The more increasing of the anti-body titer average and more decreasing CV can give the more optimal protection for H5N1 virus attack. The recomended result on this research average titer and CV is giving sarang semut extract by 10 mgs/kgs BB dosage.

James L. Januzzi, Jr, MD, FACC, Chair Luis C. Afonso, MBBS, FACC Brendan Everett, MD, FACC Adrian F. Hernandez, MD, FACC William Hucker, MD, PhD Hani Jneid, MD, FACC Dharam J. Kumbhani, MD, SM, FACC Joseph E. Marine, MD, FACC Pamela Bowe Morris, MD, FACC Robert Piana, MD, FACC Karol E.

The literature on risk factors for venous thromboembolism (VTE) is replete with complex combinations of genetic and environmental determinants. Here we apply statistical genetic models to data from Mexican Americans of South Texas participating in the San Antonio Family Study (SAFS) to help disentangle some of this complexity. The SAFS has data for nearly 50 large extended pedigrees (Figure A) that are extensively phenotyped, especially for traits related to the pathophysiology of cardiovascular disease. Using a linear mixed model approach, while accounting for age, sex, and their interactions (including age-squared, age-by-sex, and age-squared-by-sex) as confounders, we found significant heritability for plasma FII (46%; N=640; p=6.9E-12) and fibrinogen (28%; N=759; p=1.6E-06) coagulant activity levels and that the Prothrombin G20210A mutation was significantly associated with both traits (FII: p=0.002; fibrinogen: p=0.037; Figure B). We also examined a dichotomous obesity variable based on the Adult Treatment Panel III criterion of sex-specific waist circumferences (>102 cm in males; >88 cm females) denoted as OBWC. Under a threshold and liability model, we found a significant heritability of the liability of OBWC (71%; N=654; p=4.4E-08) and that Prothrombin G20210A was a significant predictor (p=0.031), while still adjusting for age, sex, and their interactions. As can be seen in Figure C, the G20210A mutation profoundly impacts the liability of OBWC such that obesity prevalence, where the prevalence parameter is denoted by Kp in the figure, increases by 27% from individuals homozygous for the major G-allele (G/G) with a prevalence of 34% to heterozygous (G/A) individuals with a prevalence of 61%. To the best of our knowledge, this appears to be the largest single-allele-dose effect for obesity reported in the literature. We next performed bivariate trait analysis (each time accounting for age, sex, and their interactions as confounders) to discover potentially meaningful correlations between the three traits of interest and to see if these would influence their association with G20210A. Under a bivariate model for any two traits, denoted as trait A and B say, the parameters of the following equation are estimated: r_p(A,B) = r_g(A,B)*sqrt(h2_A)*sqrt(h2_B) + r_e(A,B)* sqrt(1-h2_A)*sqrt(1-h2_B), where r_p(A,B), r_g(A,B), and r_e(A,B) respectively denote the phenotypic, genetic, and environmental correlation coefficients of A and B, and where h2_A and h2_B denote the trait heritabilities. Moreover, we can test their significance using likelihood-based inferential statistics. For the FII and OBWC bivariate analysis, none of the correlation coefficients were significant. For the fibrinogen and OBWC bivariate analysis, we found significant phenotypic (r_p=0.24; p=2.0E-05) and genetic (r_g=0.41; p=0.024) correlations. For the FII and fibrinogen bivariate analysis, we found significant phenotypic (r_p=0.26; p=3.1E-10) and environmental (r_e=0.28; p=0.003) correlations. For all bivariate analyses, the Prothrombin G20210A mutation was always a significant predictor for both traits of any given pair. In conclusion, Prothrombin G20210A is pleiotropically associated with plasma FII and fibrinogen coagulant activity levels, and OBWC, and profoundly impacts the prevalence of obesity in our sample. The associations of the prothrombin G20210A mutation are not affected by the consideration of inter-correlations between the three traits examined and thus appears to be fairly robust. Figure 1 Figure 1. Disclosures DeFronzo: AstraZeneca: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Novo Nordisk: Membership on an entity's Board of Directors or advisory committees; Boehringer-Ingelheim: Membership on an entity's Board of Directors or advisory committees, Research Funding; Intarcia: Membership on an entity's Board of Directors or advisory committees; Merck: Research Funding.

The aims of the study were to: determine the interaction effect of market waste bokashi fertilizer with urea dose on the growth and yield of mustard greens and determine the best dose of market waste bokashi fertilizer with various doses of urea to increase the growth and yield of mustard greens. This research was conducted using factorial RBD, namely: Factor I: P1: without bokashi; P2: 5 tons/ha; P3: 10 tons/ha; P4: 15 tons/ha, Factor II consists of Q1: No treatment; Q2: 100 kg/ha; Q3: 200 kg/ha. Data analysis using Anova and continued with LSD level 5%. The results showed that: 1) There was a single factor effect of various doses of market waste bokashi fertilizer and urea on the growth and yield of mustard greens; 2) Supply of the best market waste bokashi fertilizer with a dose of 15 tons/ha Bokashi and 20 g/m2 urea for mustard cultivation. Key Words: growth, market waste bokashi, mustard greens, urea, yield

Background Unfractionated heparin (UFH) is used for prophylaxis and treatment of thrombosis in children. Laboratory monitoring of UFH is needed to prevent over- or under-anticoagulation. Objectives Study objectives were to investigate i) the association between UFH dose and UFH effect as monitored by anti-IIa, ii) the relationship of anti-IIa and anti-Xa effects, and iii) the influence of patients’ age and other factors on UFH effect. Patients and methods Randomized controlled trial in children during cardiac catheterization, comparing high-dose UFH (100 units/kg bolus) versus low-dose UFH (50 units/kg bolus). Blood samples were drawn at baseline, after 30, 60 and 90 minutes. For the purpose of this study, 49 children and 117 blood samples were evaluated. Results The anti-IIa assay discriminated well between high and low-dose UFH. Multiple regression demonstrated a significant influence of UFH dose and age on anti-IIa levels. Younger children had lower anti-IIa levels than older children, an effect more pronounced at low-dose UFH. Anti-Xa/anti-IIa ratios were equal at low-dose UFH. However, anti-Xa levels were relatively increased over anti-IIa in infants and after high-dose UFH bolus. Conclusion The UFH effect on anti-IIa levels is lower in infants compared to older children. This influence of age appears to be dose-dependent, more pronounced at low-dose UFH. Anti-Xa versus anti-IIa levels are not equal, particularly in infants and after high dose UFH. Monitoring UFH using solely anti-Xa assays may not be sufficient in children and the anti-IIa assay may provide important complementary information. This article is protected by copyright. All rights reserved.

The research was carried out in the Tigapanah Village Farmers' field, Tigapanah Subdistrict, Karo Regency, from March to June 2016, where altitude was ± 1200 meters above sea level, Adosol land. The aim of the study was to determine the chicken manure dosage and the concentration of coconut water and its interaction with the growth and yield of Ercis plants (Pisumsativum L). Factorial Randomized Group Design (RBD) 4 levels, factor I: dose acquisition 1) without manure (control) 2) chicken manure 2) 200 g / plant, 3) 300 g / plant, 4) 400 g / plant (A0 , A1, A2, and A3). Factor II. Concentration of coconut water 4 level 1) 0 ml / plant, 2) 100 ml / plant, 3) 200 ml / plant, 4) 300 ml / plant (K0, K1, K2, K3). The results of the study showed that the effect of using chicken manure was significantly different (p ˃ 0.05) on the growth of plant height, number of branches, pods and weights of Ercis plants except that the age began to flower. Giving a dose of coconut water also gave a significantly different effect (p ˃ 0.05) on the growth of plant height, number of branches, production of pods and production weights except age began to flower. The interaction of doses of chicken manure and doses of coconut water did not have a significant effect on all observed parameters.

Lupus anticoagulant hypoprothrombinaemia syndrome (LAC-HPS) is a rare disorder, defined by the presence of an acquired factor II (FII) deficiency and lupus anticoagulant (LAC) (Rapaport et al. 1960...

bstract. The article presents a clinical case of recurrent thromboembolism of smallbranches of the pulmonary artery in a patient with a diagnosed hereditary thrombophiliaassociated with the mutation of the factor II coagulation of the blood, factor I coagulation of the blood, an antagonist of the tissue plasminogen activator, B12-dependentmethionine synthase, methylentetrahydrofolate reductase, hyperhomocysteinemia. Thesources of modern domestic and foreign literature on the problems of venousthromboembolism have been analyzed. The role of genetic research in suspicion ofthrombophilia, which can help in the diagnosis to practical physicians of a wide profile,is highlighted. Taking into account the tendency to increase the number of clinical casesof thromboembolism of small branches of the pulmonary artery, a conclusion wasreached regarding a more rigorous examination of young patients with an appropriateemphasis on the detection of polymorphism of genes in cases of suspicion ofthrombophilia.

La prevalencia del rechazo escolar es difícil de determinar debido a la discrepancia en la definición del constructo y a la falta de información nacional. En este sentido, el presente estudio tuvo como objetivo analizar las diferencias de prevalencia del rechazo escolar, entendido como la resistencia a ir a la escuela o a permanecer en esta, en función del sexo y curso académico. La muestra estuvo compuesta por 1,786 estudiantes ecuatorianos con edades comprendidas entre los 15 y los 18 años (Medad = 16.31; DE = 1.01). Se utilizó la School Refusal Assessment Scale-Revised for Children (SRAS-R-C), cuyos factores evalúan la evitación de estímulos que producen afectividad negativa (Factor I), el escape de la aversión social y/o situaciones evaluativas (Factor II), la búsqueda de atención de personas significativas (Factor III) y la búsqueda de refuerzos tangibles fuera de la escuela (Factor IV). Para establecer si existían diferencias entre el porcentaje de estudiantes clasificados con alta conducta de rechazo escolar en función del sexo y el curso académico, se utilizó la prueba Z de diferencia de proporciones. La proporción de chicas fue significativamente mayor en el Factor I, mientras que el porcentaje de chicos fue significativamente superior en el Factor IV. Las diferencias significativas en función del curso se hallaron en el Factor I entre primero y tercero y entre segundo y tercero de bachillerato, y en el Factor IV entre primero y tercero. Se discuten las implicaciones prácticas de estos hallazgos. Prevalence of school refusal is difficult to determine due to the discrepancy in the definition of the construct and the lack of national information. In this sense, the present study aimed to analyze the differences in prevalence of school refusal, understood as the resistance to go to school or to remain in it, across sex and grade. The sample consisted of 1,786 Ecuadorian students aged between 15 and 18 years (Mage = 16.31; SD = 1.01). The School Refusal Assessment Scale-Revised for Children (SRAS-RC) was used, whose factors assess the avoidance of stimuli that provoke negative affectivity (Factor I), the escape from social aversion and/or evaluative situations (Factor II), the pursuit of attention from significant others (Factor III), and the pursuit of tangible reinforcement outside of school (Factor IV). The Z test for difference in proportions was used to establish whether there were differences between the percentage of students classified with high school refusal behavior across sex and grade. The proportion of girls was significantly higher in Factor I, while the percentage of boys was significantly higher in Factor IV. Significant differences across grade were found in Factor I between first and third grade and between second and third grade of baccalaureate, and in Factor IV between first and third grade. The practical implications of these findings are discussed.

INTRODUÇÃO:, A trombofilia é uma tendência aumentada para o desenvolvimento de coágulos sanguíneos, podendo ser tanto genética como adquirida, causando assim riscos de complicações como trombose venosa, embolia pulmonar ou perdas gestacionais. Considerando que o abortamento recorrente (AR) pode ser definido como dois ou mais abortamentos consecutivos, há evidências de que as trombofilias hereditárias, principalmente as variações genéticas associadas ao fator V, fator II e MTHFR podem estar associadas a esta condição. OBJETIVO: Analisar a prevalência das trombofilias hereditárias numa amostra da população do estado do Rio Grande do Sul, representada por doadores do banco de sangue do Hospital de Clínicas de Porto Alegre (HCPA) e em mulheres com abortamentos recorrentes. METODOLOGIA: Foram examinadas 325 amostras de doadores de sangue do banco de sangue do HCPA e 33 amostras de mulheres com casos de abortamento recorrente atendidas entre os anos de 2014 a 2018 no Ambulatório Pré-Natal do HCPA. O DNA dos doadores foi extraído de papel-filtro, enquanto o material genético das mulheres com AR já estava armazenado em biorepossitório. O grupo de mulheres com AR foi pareado por idade com um grupo controle de 99 mulheres doadoras de sangue para um estudo caso-controle. A genotipagem de todas as amostras foi realizada por PCR qualitativo em Tempo Real com sondas específicas para cada variante - FV g.1691G>A, FII g.2021G>A, MTHFR g.677C>T, MTHFR g.1298A>C. RESULTADOS: No grupo de doadores de sangue, onde a média de idade foi de 38.5 (dp ± 11.4) anos, as frequências alélicas de cada variante genética foram de FV g.1691G>A 96.3% (G), 3.7% (A); FII g.2021G>A 95.7% (G), 4.3% (A); MTHFR g.677C>T 44.3% (C), 42.2% (T); MTHFR g.1298A>C 55.4% (A), 39.1% (C). No grupo caso de mulheres com AR a idade média foi de 35 (dp ±5.7) anos, as frequências alélicas de cada variante genética foram de FV g.1691G>A 100% (G), 0% (A); FII g.2021G>A 98.5% (G), 1.5% (A); MTHFR g.677C>T 76% (C), 24% (T); MTHFR g.1298A>C 80% (A), 20% (C). Quando comparamos o grupo caso, com o grupo controle (composto de mulheres retiradas do grupo de doadores de sangue e pareado por idade) verificamos que a prevalência das mutações nos genes FV e FII foi exatamente a mesma nos dois grupos, com nenhum resultado em homozigose para as mutações. Os resultados para MTHFR, apesar de diferirem em prevalência, não apresentaram associação significativa entre os grupos caso e controle. CONCLUSÃO: Os resultados da amostra populacional de doadores de sangue descrevem as frequências das variantes associadas a trombose venosa profunda e hiperhomocisteinemia, apresentando de forma importante um conhecimento sobre o perfil genético dos doadores de sangue sul-brasileiros. Para ambas as análises, acreditamos que um aumento no tamanho da amostra é necessário para melhor esclarecer algumas interpretações. No entanto, este estudo contribui para aumentar o conhecimento da frequência das variantes genéticas analisadas em um grupo de indivíduos da população do sul do Brasil. INTRODUCTION: Both genetically and acquired, thrombophilia is an increased tendency for the development of blood clots, thus causing risks of complications such as venous thrombosis, pulmonary embolism or gestational loss. Considering that recurrent miscarriage (RM) can be defined as two or more consecutive miscarriages, there is evidence that hereditary thrombophilias, especially genetic variations associated with factor V, factor II and MTHFR, may be associated with this condition. OBJECTIVE: The aim of this study was to analyze the prevalence of hereditary thrombophilias in a population sample from the state of Rio Grande do Sul, represented by blood bank donors from the Hospital de Clínicas de Porto Alegre (HCPA), and in women with recurrent miscarriages. METHODOLOGY: We examined 325 blood donor samples from the HCPA blood bank and 33 samples from women with recurrent miscarriage attended between 2014 and 2018 at the HCPA Prenatal Outpatient Clinic. DNA of donors was extracted from filter paper, while the genetic material of women with RM was already stored in a biorepository. The group of women with RM was age matched with a control group of 99 female blood donors for a case-control study. Genotyping of all samples was performed by qualitative Real-Time PCR with probes specific for each variant - FV g.1691G>A, FII g.2021G>A, MTHFR g.677C>T, MTHFR g.1298A>C. RESULTS: In the group of blood donors, where the mean age was 38.5 (sd ± 11.4) years, the allele frequencies of each genetic variant were FV g.1691G> A 96.3% (G), 3.7% (A); FII g.2021G> 95.7% (G), 4.3% (A); MTHFR g.677C> T 44.3% (C), 42.2% (T); MTHFR g.1298A> C 55.4% (A), 39.1% (C). In the case of women with RA the mean age was 35 (sd ± 5.7) years, the allele frequencies of each genetic variant were FV g.1691G> A 100% (G), 0% (A); FII g.2021G> 98.5% (G), 1.5% (A); MTHFR g.677C> T 76% (C), 24% (T); MTHFR g.1298A> C 80% (A), 20% (C). When comparing the case group with the control group of women withdrawn from the age-matched blood donor group we found that the prevalence of mutations in the FV and FII genes was exactly the same in the two groups, with no homozygous results for the mutations. The results for MTHFR, although differing in prevalence, did not present a statistical significant association between the case and control groups. CONCLUSION: Results of the population sample of blood donors describe the frequencies of the variants associated with deep venous thrombosis and hyperhomocysteinemia, presenting important knowledge about the genetic profile of blood donors in South Brazil. Regarding the screening of these alterations in women with RM, the data suggest that there is no association. For both analyzes, we believe that an increase in sample size is necessary to better clarify some interpretations. Nevertheless, this study contributes to increase the knowledge of the frequency of the genetic variants analyzed in a group of individuals of the population of south Brazil.

espanolObjetivo: explorar la actitud y los sentimientos de estudiantes de Enfermeria hacia los cuidados ante la muerte, asi como las diferencias segun las caracteristicas demograficas, la experiencia previa con la muerte y la realizacion de practicas clinicas. Metodo: estudio descriptivo transversal en estudiantes de Enfermeria de universidades de Galicia a traves de la escala validada FATCOD-S. Otras variables: edad, sexo, escuela/facultad, curso academico, entorno residencial en la infancia, vivencia de experiencia previa con el final de la vida y si ya habian realizado practicas clinicas. Se incluyo una pregunta abierta acerca de sus sentimientos ante el fenomeno de estudio. Analisis bivariante mediante t de Student, ANOVA o U de Mann-Whitney y coeficiente de correlacion de Spearman. Resultados: se obtuvo respuesta de 463 estudiantes. La puntuacion media global de la escala FATCOD fue de 98,1 puntos. Quienes tenian 20 anos o menos del curso 1o tenian valores estadisticamente mas bajos que los mayores, tanto en el factor I (p= 0,014) como en el II (p= 0,04); tenian puntuaciones mas altas en ambos factores (p= 0,02) si declaraban haber tenido experiencias previas. Se encontro un gradiente creciente entre las puntuaciones de ambos factores y el curso academico (p Conclusiones: los estudiantes de Enfermeria gallegos presentaron una actitud positiva hacia los cuidados al final de la vida. La edad y la experiencia previa resultaron ser factores clave, asi como la realizacion de practicas clinicas EnglishObjective: to explore the attitude and feelings of Nursing students towards care at death, as well as differences according to demographical characteristics, previous experience with death, and clinical practices completed. Method: a descriptive cross-sectional study on Nursing students from Universities of Galicia, through the validated FATCOD-S scale. Other variables were: age, gender, school, academic course, residential environment at childhood, previous experience with end of life, and if they had already completed clinical practices. An open question was included regarding their feelings towards the subject of the study. Bivariate analysis was conducted through Student’s t, ANOVA or Mann-Whitney’s U tests, and Spearman Correlation Coefficient. . Results: there were answers by 463 students. The overall mean score in the FATCOD scale was 98.1. Students in their 1st year who were ≤20-year-old presented statistically lower values than older students, both in Factor I (p= 0.014) and in Factor II (p= 0.04); they had higher scores in both factors (p= 0.02) if they declared having had previous experiences. An increasing gradient was found between the scores in both factors and the academic year (p Conclusions: nursing students in Galicia presented a positive attitude towards end-of-life care. Age and previous experience represented key factors, as well as having completed clinical practices.

This study aims: to determine the appropriate length of fermentation time for nipah fruit skin and nipah midrib so that the nutritional value and digestibility of cellulose and hemicellulose are increased. The study used a completely randomized design with 2 factor, namely: the type of material and the length of time of fermentation and was repeated 3 times. The first factor is the type of material: nipah fruit skin and palm fronds. Factor II, namely the lenght of fermentation time : 5 days, 10 days, 15 days and 20 hari hearts. Variables measured were crude fiber content, crude protein, cellulose digestibility and hemicellulose digestibility. The results showed that the digestibility of cellulose and hemicellulose digestibility of nipah fruit peels and palm fronds were significantly different (P

Introduction Hemodialysis (HD) causes many life changes, not only for patients undergoing it but also for their families by allowing them to rely on this lifesaving equipment unless they receive a kidney transplant. The stress of the primary caregivers, who spends the most time in the family taking care of the patient undergoing HD, is quite high. This study was to identify attitudes about stress and coping among primary caregivers of HD patients. Methods Q-methodology was undertaken because it integrates quantitative and qualitative research methods. A convenience sample of 33 primary caregivers of HD patients participated. Forty selected Q-samples were obtained from each participant and were classified into a forced normal distribution using a nine-point grid. Data was analyzed using a pc-QUANL program. Findings Three discrete factors emerged as follows: Factor I (they reduced their stress by participating in religious activities; religious sublimation), Factor II (they always worried about the caregiving situations and about the patients' conditions; nervousness), and Factor III (they thought it better to accept their stressful situations; leading handler). Three factors accounted for 44.5% of all the variance, including Factor I (26.0%), Factor II (10.1%), and Factor III (8.4%). The eigenvalues were 8.58, 3.34, and 2.79, respectively. Discussion The subjectivities of the three factors that were identified can be applied during the planning stages of effective interventions for stress and coping. Healthcare workers in clinical practices should consider assesses primary caregivers' attitudes about stress and coping and approaches their situation to cope with it and to adapt to lifestyle changes.

The aim of the undertaken studies was to determine the effect of the herbicides used in the experiment on important features of the consumption values of three varieties of edible potato. Test results come from a field experiment conducted in 2007–2010. The experiment was established as two-factor in the split-plot system in three repetitions. Two factors were tested in the experiment: factor I - potato varieties: Satina, Tajfun, Cekin; factor II – five methods of weed control. Darkening of the pulp of cooked tubers after 10 min, 2 and 24 hours shape the ways of weed control of the plantation and the genotype of the grown varieties. The use of herbicides in 2–5 variants has significantly increased the darkening of the pulp. The Tajfun variation darkened to the lowest degree, and Cekin to the largest degree.