51. Peptidic sequence 'HSEAETGPP' is recognized by the sera of pars planitis patients
- Author
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Juan C. Cancino-Diaz, Miguel Pedrosa-Seres, Sandra Rodríguez-Martínez, Mario E. Cancino-Diaz, Jorge Ismael Castañeda-Sánchez, and Everardo Curiel-Quesada
- Subjects
Phage display ,business.industry ,T cell ,Enzyme-Linked Immunosorbent Assay ,General Medicine ,Human leukocyte antigen ,Pars Planitis ,medicine.disease ,Polymerase Chain Reaction ,medicine.anatomical_structure ,Antigen ,Peptide Library ,Immunology ,Medicine ,Intermediate uveitis ,Humans ,Amino Acid Sequence ,business ,Peptides ,HLA-DRB1 ,CD8 - Abstract
Purpose: HLA class II, p-36 protein, heat shock protein and retinal antigens have been associated with pars planitis (PP), but their participation in the development of the disease are unknown. A search for new molecules related to PP is necessary. This work focused on the identification of peptides recognized by PP patient sera using the phage display method. Methods: Sera of PP patients were used to isolate peptides fused to M13-phage pIII protein. The response of PP and healthy sera to peptides was determined by ELISA. PCR amplification and sequencing of peptide-encoding fragments from clones with high recognition by PP sera were used to characterize displayed peptides. Results: One hundred clones were randomly selected from a phage display library after three panning rounds using serum proteins from a PP patient. The immunologic response level of 100 clones selected were determined with a major number of patients, it was found that one clone was recognized stronger in PP patients sera than in healthy sera (PP vs. healthy; P