51. Small Peptides Derived from Penetratin as Antibacterial Agents.
- Author
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Parravicini O, Somlai C, Andujar SA, Garro AD, Lima B, Tapia A, Feresin G, Perczel A, Tóth G, Cascales JL, Rodríguez AM, and Enriz RD
- Subjects
- Anti-Bacterial Agents chemical synthesis, Anti-Bacterial Agents pharmacology, Cell-Penetrating Peptides, Escherichia coli drug effects, Escherichia coli isolation & purification, Humans, Microbial Sensitivity Tests, Molecular Dynamics Simulation, Oligopeptides chemical synthesis, Oligopeptides pharmacology, Protein Conformation, Salmonella drug effects, Staphylococcus aureus drug effects, Anti-Bacterial Agents chemistry, Carrier Proteins chemistry, Oligopeptides chemistry
- Abstract
The synthesis, in vitro evaluation and conformational study of several small-size peptides acting as antibacterial agents are reported. Among the compounds evaluated, the peptides Arg-Gln-Ile-Lys-Ile-Trp-Arg-Arg-Met-Lys-Trp-Lys-Lys-NH2 , Arg-Gln-Ile-Lys-Ile-Arg-Arg-Met-Lys-Trp-Arg-NH2 , and Arg-Gln-Ile-Trp-Trp-Trp-Trp-Gln-Arg-NH2 exhibited significant antibacterial activity. These were found to be very active antibacterial compounds, considering their small molecular size. In order to better understand the antibacterial activity obtained for these peptides, an exhaustive conformational analysis was performed, using both theoretical calculations and experimental measurements. Molecular dynamics simulations using two different media (water and trifluoroethanol/water) were employed. The results of these theoretical calculations were corroborated by experimental circular dichroism measurements. A brief discussion on the possible mechanism of action of these peptides at molecular level is also presented. Some of the peptides reported here constitute very interesting structures to be used as starting compounds for the design of new small-size peptides possessing antibacterial activity., (© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2016
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