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578 results on '"Enjoji M"'

59. Small cell carcinoma of the esophagus.

Author

Mori, Masaki, Matsukuma, Akito, Adachi, Yosuke, Miyagahara, Toshiro, Matsuda, Hiroyuki, Kuwano, Hiroyuki, Sugimachi, Keizo, Enjoji, Munetomo, Mori, M, Matsukuma, A, Adachi, Y, Miyagahara, T, Matsuda, H, Kuwano, H, Sugimachi, K, and Enjoji, M

Published
1989
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91. N-myc gene product expression in neuroblastoma.

Author

Hashimoto, H, Daimaru, Y, Enjoji, M, and Nakagawara, A

Abstract

The presence and distribution of N-myc gene product were studied in 13 neuroblastomas and five ganglioneuroblastomas, using immunohistochemical techniques. Nine tumors (eight neuroblastomas and one ganglioneuroblastoma of composite type) contained neuroblastoma cells with positive nuclei for N-myc protein. Microscopic examination showed that most of the positive neuroblastoma cells seemed to be immature, with no apparent neuronal differentiation. Nine of 11 tumours with amplified N-myc gene copies exhibited tumour cells with positive immunostaining for the N-myc gene product, while none of the seven non-N-myc amplified cases contained immunoreactive tumour cells. The survival of the patients positive for N-myc protein was significantly low compared with that of the negative ones. It is concluded that immunohistochemical staining for the N-myc gene product will facilitate prediction of the prognosis of patients with neuroblastoma. [ABSTRACT FROM PUBLISHER]

Published
1989
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92. Distribution of basement membrane antigens in clinical gastric adenocarcinomas: an immunohistochemical study.

Author

Nakamura, K, Mori, M, and Enjoji, M

Abstract

The distribution of laminin and collagen type IV in the basement membranes of 85 gastric adenocarcinomas was studied using immunoperoxidase techniques to check for invasive carcinoma. Lymph nodes with metastases were also studied in 23 cases. Thick and discontinuous staining of the basement membranes was observed in 12 cases of well differentiated adenocarcinoma; thin and discontinuous staining in 26 (12 well and 14 moderately differentiated adenocarcinomas); fragmentary staining in 36 (15 moderately and 21 poorly differentiated adenocarcinomas); and unrecognisable basement membrane staining in the remaining 11 cases of poorly differentiated adenocarcinoma. These patterns were largely related to the histological grade, the nuclear atypism and loss of polarity of tumour cells, and the degree of inflammatory infiltration. [ABSTRACT FROM PUBLISHER]

Published
1987

99. Hammerhead ribozyme cleavage of apolipoprotein B mRNA generates a truncated protein.

Author

Wang, J P, Enjoji, M, Tiebel, M, Ochsner, S, Chan, L, and Teng, B B

Abstract

Target substrate-specific hammerhead ribozyme cleaves the specific mRNA and results in the inhibition of gene expression. In humans, overproduction of apolipoprotein B (apoB) is positively associated with premature coronary artery diseases. To modulate apoB gene expression, we designed hammerhead ribozymes targeted at AUA(6665) and GUA(6679) of apoB mRNA, designated RB16 and RB15, respectively, and investigated their effects on apoB mRNA in HepG2 cells. The results demonstrated that RB15 and RB16 ribozyme RNAs cleaved apoB RNA efficiently in vitro. Both ribozymes, RB15 and RB16, were used to construct recombinant adenoviral vectors, designated AvRB15 and AvRB16, respectively, for in vivo gene transfer. HepG2 cells were infected with 2 x 10(5) plaque-forming units of AvRB15 for 5, 10, 15, and 24 h. An RNase protection assay showed that the expression of the RB15 transcript was time-dependent; it increased approximately 300-fold from 5 to 24 h. Using reverse ligation-mediated polymerase chain reaction, the 3' cleavage product of apoB mRNA was detected, and the exact cleavage site of apoB mRNA was confirmed by sequencing. Importantly, the levels of apoB mRNA in HepG2 cells decreased approximately 80% after AvRB15 infection. Pulse/chase experiments on HepG2 cells treated with AvRB15 and AvRB16 demonstrated that ribozyme cleavage produced a truncated protein that was secreted at a density of 1. 063-1.210 g/ml. The cleavage activity of RB15 on apoB mRNA was more efficient than that of RB16. Moreover, pulse/chase experiments in HepG2 cells treated with AvRB15 revealed that most of the truncated apoB protein was degraded intracellularly. We conclude that hammerhead ribozyme targeted at GUA(6679) of apoB mRNA cleaves apoB mRNA, results in decreased apoB mRNA levels, and generates a truncated apoB of the expected size in vivo. Thus, the therapeutic application of ribozyme in regulating apoB production holds promise.

Published
1999

100. Malignant neuroepithelioma (peripheral neuroblastoma). A clinicopathologic study of 15 cases

Author

Hiroshi Hashimoto, Kiryu H, Daimaru Y, Enjoji M, and Nakajima T

Subjects
Adult, Male, Pathology, medicine.medical_specialty, Rosette Formation, Adolescent, Enolase, Pathology and Forensic Medicine, Peripheral Nervous System Neoplasms, Peripheral Neuroblastoma, Medicine, Humans, Neuroectodermal Tumors, Primitive, Peripheral, Child, Neurons, business.industry, Histocytochemistry, Soft tissue, Extremities, medicine.disease, Staining, Isoenzymes, Child, Preschool, Phosphopyruvate Hydratase, Alveolar rhabdomyosarcoma, Immunohistochemistry, Surgery, Female, Sarcoma, Anatomy, Differential diagnosis, business, Follow-Up Studies
Abstract

A clinicopathologic study of 15 cases of malignant neuroepithelioma (peripheral neuroblastoma) of soft tissues is reported. The patients were chiefly young Japanese adults with a median age of 21 years. The tumors arose mainly in the soft tissues of the lower extremity (seven cases) and the trunk (four cases). Microscopically, there were sheets of closely packed, small round or oval cells, and Homer Wright-type rosettes were seen in all cases, one of which also had Flexner-type rosettes. Immunohistochemical cytoplasmic localization of neuron-specific enolase (NSE) was demonstrated in six of the eight cases, using the peroxidase-antiperoxidase (PAP) method. In no case, however, was there any staining reaction for S-100 protein. Of the 14 patients for whom follow-up information could be obtained, nine died within a period of 2 years and two were alive and well for over 5 years after the initial treatment. Differential diagnosis from other soft-tissue round-cell sarcomas, such as embryonal or alveolar rhabdomyosarcoma, extraskeletal Ewing's sarcoma, and others, are briefly discussed, on a clinicopathologic basis.

Published
1983

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