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We use deep narrowband Ca H&K ($F395N$) imaging taken with the Hubble Space Telescope (HST) to construct the metallicity distribution function (MDF) of Local Group (LG) ultra-faint dwarf (UFD) galaxy Eridanus II (Eri II). When combined with archival $F475W$ and $F814W$ data, we measure metallicities for 60 resolved red giant branch stars as faint as $m_{F475W}\sim24$ mag, a factor of $\sim4$x more stars than current spectroscopic MDF determinations. We find that Eri II has a mean metallicity of [Fe/H]$=$-2.50$^{+0.07}_{-0.07}$ and a dispersion of $��_{\mbox{[Fe/H]}}=0.42^{+0.06}_{-0.06}$, which are consistent with spectroscopic MDFs, though more precisely constrained owing to a larger sample. We identify a handful of extremely metal-poor star candidates (EMP; [Fe/H] $< -3$) that are marginally bright enough for spectroscopic follow up. Eri II's MDF appears well-described by a leaky box chemical evolution model. We also compute an updated orbital history for Eri II using Gaia eDR3 proper motions, and find that it is likely on first infall into the Milky Way. Our findings suggest that Eri II underwent an evolutionary history similar to that of an isolated galaxy. Compared to MDFs for select cosmological simulations of similar mass galaxies, we find that Eri II has a lower fraction of stars with [Fe/H] $< -3$, though such comparisons should currently be treated with caution due to a paucity of simulations, selection effects, and known limitations of Ca H&K for EMPs. This study demonstrates the power of deep HST CaHK imaging for measuring the MDFs of UFDs., Resubmitted to ApJ with revisions following a positive referee report of the initial draft; 29 pages, 19 figures, and 3 tables, including appendix. Comments welcome!

Price is the most important factor influencing demand rate based on marketing and economic theory. Along with price, stock display is also a major factor, as displayed stocks may induce customers to purchase more due to its visibility. Moreover, the demand for perishable products depends on its freshness. However, relatively little devotion has been paid to the influence of expiration dates despite the fact that they are an important factor in consumers’ purchase decisions. As a result, we develop an inventory model for perishable products in which demand explicitly in a multivariate function of price, displayed stocks, and expiration dates. We then formulate the model by determining the optimal selling price to maximize the total profit by using classical optimization method with the necessary condition given by Kuhn-Tucker. Furthermore, we discuss the optimal decisions under two scenarios: upper bound of profit and lower bound of profit by taking holding cost as a function of upper and lower bound respectively. Finally, a numerical example is demonstrated along with sensitivity analysis to describe the impact of inventory parameters on the optimal decisions.

We present the first proper motion (PM) measurements for the dwarf elliptical galaxies NGC 147 and NGC 185, two satellite galaxies of M31, using multi-epoch HST imaging data with time baselines of $\sim 8$ years. For each galaxy, we take an error-weighted average of measurements from HST ACS/WFC and WFC3/UVIS to determine the PMs. Our final results for the PMs are $(\mu_\mathrm{W}, \mu_\mathrm{N})_\mathrm{N147} = (-0.0232, 0.0378) \pm (0.0143, 0.0146)\,\mathrm{mas}\,\mathrm{yr}^{-1}$ for NGC 147, and $(\mu_\mathrm{W}, \mu_\mathrm{N})_\mathrm{N185} = (-0.0242, 0.0058) \pm (0.0141, 0.0147)\,\mathrm{mas}\,\mathrm{yr}^{-1}$ for NGC 185. The 2-dimensional direction of motion for NGC 147 about M31 is found to be aligned with its tidal tails. The 3-d positions and velocities of both galaxies are transformed into a common M31-centric coordinate system to study the detailed orbital histories of the combined M31+NGC 147+NGC 185 system via numerical orbit integration. We find that NGC 147 (NGC 185) had its closest passage to M31 0.3-0.5~Gyr ($\gtrsim 1.6$~Gyr) within the past 6 Gyr at distances of $\sim 70$ kpc (70-260 kpc). The pericentric times of NGC 147/NGC 185 correlate qualitatively well with the presence/absence of tidal tails seen around the galaxies. Our PMs show that the orbital poles of NGC 147, and also NGC 185 albeit to a lesser degree, agree within the uncertainties with the normal of the Great Plane of Andromeda (GPoA). These are the first measurements of the 3-d angular momentum vector of any satellite identified as original GPoA members. Our results strengthen the hypothesis that the GPoA may be a dynamically coherent entity. We revisit previous claims that NGC 147 and NGC 185 are binary galaxies and conclude that it is very unlikely the two galaxies were ever gravitationally bound to each other., Comment: 20 pages, 9 figures, 4 tables, accepted for publication in ApJ

In the last decade, Matrix Assisted Laser Desorption Ionisation Mass Spectrometry (MALDI MS) has proven to be a valuable analytical tool in forensic research as it can detect and map molecular information of forensic relevance in trace evidence such as fingermarks and hair. The first published proof of concept demonstrating that it was possible to differentiate males and females from the peptide and protein content of their fingermarks was published in 2012. In that work, MALDI MS was used in Profiling mode (MALDI MSP) to quickly obtain spectral profiles of ungroomed marks. These were submitted to Partial Least Square Discriminant Analysis (PLS-DA) yielding sex discrimination with an accuracy between 67.5% and 74.4%, if harsh classification criteria were applied. Since then, this research has progressed to investigate the opportunity to increase the accuracy of prediction in natural marks (obtained with no preparation of the fingertip prior to deposition) either unenhanced or enhanced prior to matrix application and MALDI analysis. Extensive statistical modelling has been employed to determine the model with the highest sex predictive accuracy. Results show that in natural marks the presence of polymers (as external contaminants) in fingermarks affects the peptide/protein signals to various degrees and, only, by using one type of scoring system, a method has been identified to provide up to 86.1% predictive power in discriminating female from male marks.

With the release of Gaia DR2, it is now possible to measure the proper motions (PMs) of the lowest mass, ultra-faint satellite galaxies in the Milky Way's (MW) halo for the first time. Many of these faint satellites are posited to have been accreted as satellites of the Magellanic Clouds (MCs). Using their 6-dimensional phase space information, we calculate the orbital histories of 13 ultra-faint satellites and five classical dwarf spheroidals in a combined MW+LMC+SMC potential to determine which galaxies are dynamically associated with the MCs. These 18 galaxies are separated into four classes: i.) long-term Magellanic satellites that have been bound to the MCs for at least the last two consecutive orbits around the MCs (Carina 2, Carina 3, Horologium 1, Hydrus 1); ii.) Magellanic satellites that were recently captured by the MCs $, Comment: 32 pages, 11 figures. Accepted to ApJ

PROBLEM Residency programs must provide training in patient safety. Yet, significant gaps exist among published patient safety curricula. The authors developed a rotation designed to be scalable to an entire residency, built on sound pedagogy, aligned with hospital safety processes, and effective in improving educational outcomes. APPROACH From July 2015 to May 2017, each second-year resident completed the two-week rotation. Residents engaged the foundational science asynchronously via multiple modalities and then practiced applying key concepts during a mock root cause analysis. Next, each resident performed a special review of an actual adverse patient event and presented findings to the hospital's Special Review Committee (SRC). Multiple educational outcomes were assessed, including resident satisfaction and attitudes (postrotation survey), changes in knowledge via pre- and posttest, quality of the residents' written safety analyses and oral presentations (per survey of SRC members), and organizational changes that resulted from the residents' reviews. OUTCOMES Twenty-two residents completed the rotation. Most components were rated favorably; 80% (12/15 respondents) indicated interest in future patient safety work. Knowledge improved by 44.3% (P < .0001; pretest mean 23.7, posttest mean 34.2). Compared to faculty, SRC members rated the quality of residents' written reviews as superior and the quality of the rated oral presentations as either comparable or superior. The reviews identified a variety of safety vulnerabilities and led to multiple corrective actions. NEXT STEPS The authors will evaluate the curriculum in a controlled trial with better measures of change in behavior. Further tests of the curriculum's scalability to other contexts are needed.

Space observatories like the Hubble Space Telescope and Gaia are providing unprecedented 6D phase space information of satellite galaxies. Such measurements can shed light on the structure and assembly history of the Local Group, but improved statistical methods are needed to use them efficiently. Here we illustrate such a method using analogs of the Local Group's two most massive satellite galaxies, the Large Magellanic Cloud (LMC) and Triangulum (M33), from the Illustris dark-matter-only cosmological simulation. We use a Bayesian inference scheme combining measurements of positions, velocities, and specific orbital angular momenta ( j ) of the LMC/M33 with importance sampling of their simulated analogs to compute posterior estimates of the Milky Way (MW) and Andromeda's (M31) halo masses. We conclude the resulting host halo mass is more susceptible to bias when using measurements of the current position and velocity of satellites, especially when satellites are at short-lived phases of their orbits (i.e. at pericentre). Instead, the j value of a satellite is well-conserved over time and provides a more reliable constraint on host mass. The inferred virial mass of the MW (M31) using j of the LMC (M33) is $\rm M_{vir, MW}=1.02^{+0.77}_{-0.55}\times10^{12}\; M_{\odot}$ ($\rm M_{vir, M31}=1.37^{+1.39}_{-0.75}\times10^{12}\; M_{\odot}$). Choosing simulated analogs whose j values are consistent with the conventional picture of a previous (< 3 Gyr ago), close encounter (< 100 kpc) of M33 about M31 results in a very low virial mass for M31 ($\rm\sim\!10^{12}\; M_{\odot}$). This supports the new scenario put forth in Patel et al. (2017), wherein M33 is on its first passage about M31 or on a long period orbit. We conclude that this Bayesian inference scheme, utilising satellite j, is a promising method to reduce the current factor of two spread in the mass range of the MW and M31 moving forward., 24 pages, 9 figures. Submitted to MNRAS

High precision proper motion (PM) measurements are available for approximately 20% of all known dwarf satellite galaxies of the Milky Way (MW). Here we extend the Bayesian framework of Patel et al. (2017b) to include all MW satellites with measured 6D phase space information and apply it with the Illustris-Dark simulation to constrain the MW's mass. Using the properties of each MW satellite individually, we find that the scatter among mass estimates is reduced when the magnitude of specific orbital angular momentum (j) is adopted rather than their combined instantaneous positions and velocities. We also find that high j satellites (i.e. Leo II) constrain the upper limits for the MW's mass and low j satellites rather than the highest speed satellites (i.e. Leo I and LMC), set the lower mass limits. When j of all classical satellites is used to simultaneously estimate the MW's mass, we conclude the halo mass is $0.85^{+0.23}_{-0.26}\times 10^{12} \rm \, M_{\odot}$ (including Sagittarius dSph) and $0.96^{+0.29}_{-0.28} \times 10^{12} \rm \, M_{\odot}$ (excluding Sagittarius dSph), cautioning that low j satellites on decaying orbits like Sagittarius dSph may bias the distribution. These estimates markedly reduce the current factor of two spread in the mass range of the MW. We also find a well-defined relationship between host halo mass and satellite j distribution, which yields the prediction that upcoming PMs for ultra-faint dwarfs should reveal j within $ 5\times10^3 - 10^4$ kpc km s$^{-1}$. This is a promising method to significantly constrain the cosmologically expected mass range for the MW and eventually M31 as more satellite PMs become available., 20 pages, 7 figures, submitted to ApJ

Background The Infection and Treatment Method (ITM) of vaccination is the only immunization procedure currently available to protect cattle against East Coast fever (ECF), a tick-transmitted disease responsible for losses of several hundreds of millions of dollars per year in sub-Saharan Africa. The vaccine comprises a homogenized preparation of infected ticks packaged in straws and stored in liquid nitrogen. The current manufacturing protocol results in straws containing 30–40 doses (ILRI 0804), which is impractical for immunizing small herds as found in dairy and smallholder farming systems. The ILRI 0804 SD stabilate was prepared as a 1:5 dilution of the parent stabilate, with the aim of producing vaccine stabilate straws containing between four to eight doses and thus suitable for smallholder farming systems. Infectivity of the diluted stabilate was assessed and the protective efficacy of the diluted stabilate was determined by performing experimental and field immunizations. Results Two groups of six cattle were inoculated with 1 ml of the diluted stabilate at 1:20 (equivalent to the recommended field dose for ILRI 0804, assuming no loss of sporozoite viability during thawing and refreezing) and 1:14 (assuming 30–35% loss of sporozoite viability). Schizonts were detected in all 12 animals, showing viability of sporozoites. Ten animals from the infectivity study and two control animals not previously exposed to T. parva were challenged with the parental ILRI 0804 stabilate. The results show that the two control animals displayed severe ECF reactions and were treated 14 days after challenge. Of the previously infected animals, only one underwent a severe reaction following challenge, a result in accord with the challenge experiments performed previously with the parent stabilate [Ticks Tick-Borne Dis 7:306-314, 2016]. The animal that displayed a severe reaction had no detectable schizonts and did not seroconvert following the initial inoculation with ILRI 0804 SD. In addition, 62 animals immunized under field conditions showed a mean seroconversion rate of 82%. Conclusion The results presented in this article demonstrate that it is possible to prepare straws suitable for use in smallholder herds by thawing, diluting and refreezing already packaged vaccine.

A bottom up in situ proteomic method has been developed enabling the mapping of multiple blood signatures on the intact ridges of blood fingermarks byMatrix Assisted Laser Desorption Mass Spectrometry Imaging (MALDI-MSI). This method, at a proof of concept stage, builds upon recently published work demonstrating the opportunity to profile and identify multiple blood signatures in bloodstains via a bottom up proteomic approach. The present protocol addresses the limitation of the previously developed profiling method with respect to destructivity;\ud destructivity should be avoided for evidence such as blood fingermarks, where the ridge detail must be preserved in order to provide the associative link between the biometric information and the events of bloodshed. Using a blood mark reference model, trypsin concentration and spraying conditions have been optimised within the technical constraints of the depositor eventually employed; the application of MALDI-MSI and Ion Mobility MS have enabled the detection, confirmation and visualisation of blood signatures directly onto the ridge pattern.\ud These results are to be considered a first insight into a method eventually informing investigations (and judicial debates) of violent crimes in which the reliable and non-destructive detection and mapping of blood in fingermarks is paramount to reconstruct the events of bloodshed.

Blood evidence is frequently encountered at the scene of violent crimes and can provide valuable intelligence\ud in the forensic investigation of serious offences. Because many of the current enhancement\ud methods used by crime scene investigators are presumptive, the visualisation of blood is not always\ud reliable nor does it bear additional information. In the work presented here, two methods employing a\ud shotgun bottom up proteomic approach for the detection of blood are reported; the developed protocols\ud employ both an in solution digestion method and a recently proposed procedure involving immobilization\ud of trypsin on hydrophobin Vmh2 coated MALDI sample plate. The methods are complementary as whilst one yields more identifiable proteins (as biomolecular signatures), the other is extremely rapid (5 minutes).\ud Additionally, data demonstrate the opportunity to discriminate blood provenance even when two different blood sources are present in a mixture. This approach is also suitable for old bloodstains which had been previously chemically enhanced, as experiments conducted on a 9-year-old bloodstain deposited on a ceramic tile demonstrate.

In inventory management, deterioration has a great impact on total profit. To develop business strategies in competitive market, stock-dependent and price-sensitive demand rate is considered to attract more customers. The holding cost and deterioration rate are time-dependent. The product considered has fixed life-time. The model considers the trade-credit policy that is the retailer will offer a certain time period to the customers to settle the account to increase the sales during the permissible period. The objective is to maximise the total cost with respect to cycle time and selling price. The classical optimisation method is used to find the optimal solution. A sensitivity analysis of optimal solutions for different inventory parameters is carried out. The managerial insights are deduced for the retailer.

We quantify the frequency of companions of low redshift ($0.013 < z < 0.0252$), dwarf galaxies ($2 \times 10^8$ M$_\odot 55'', projected separations r$_p < 150$ kpc and relative line-of-sight velocities $\Delta V_{\rm LOS} < 150$ km/s. While the mock catalogs predict a factor of 2 more isolated dwarfs than observed in SDSS, the mean number of observed companions per dwarf is $N_c \sim 0.04$, in good agreement with Illustris when accounting for SDSS sensitivity limits. Removing these limits in the mock catalogs predicts $N_c\sim 0.06$ for future surveys (LSST, DESI), which will be complete to M$_* = 2\times 10^8$ M$_\odot$. The 3D separations of mock dwarf multiples reveal a contamination fraction of $\sim$40% in observations from projection effects. Most isolated multiples are pairs; triples are rare and it is cosmologically improbable that bound groups of dwarfs with more than 3 members exist within the parameter range probed in this study. We find that $$1:4) steadily increases with decreasing Primary stellar mass, whereas the cosmological "Major Merger rate'' (per Gyr) has the opposite behaviour. We conclude that cosmological simulations can be reliably used to constrain the fraction of dwarf mergers across cosmic time., Comment: 21 pages, 16 Figures, submitted to MNRAS (Referee stage ; Comments are welcome)

During the intra-uterine life the umbilical cord serves as an only one source for nutrition, energy and growth of the fetus but, during extra uterine life the umbilical cord stump serves as a commonest site for the spread of infection due to warmth and blood circulation over it. The country like India having most area of lower economic status requires the easily available, cheap and convenient method which mothers themselves also can perform to prevent the occurrence of infection. Hence, this study was conducted to evaluate the effect of application of breastmilk on separation time of umbilical cord among newborn. Methodology: The Quasi experimental Posttest only control group design was used to do the pilot study using a quantitative approach. The study was conducted at Raj Nursing Home, Anand and Himalaya hospital, Anand. Convenient sampling technique was used to select 10 newborns in experimental group & control group (5 in each). The tool of data collection included validated checklist of neonatal bio-demographic variables and of physiological parameters of signs of cord separation. The data collection tool was divided in to 2 sections which are Observation checklist of Neonatal variables, existing condition of cord (at birth) and observation checklist on physiological parameters signs of cord separation. Results: The data was analyzed using descriptive and inferential statistics. The result shows there is reduction in umbilical cord separation time of experimental group (mean 4.8 days) as compare to control group (7.4 days).This shows there is a impact of topical application of breastmilk on umbilical cord separation time. Conclusion: There is significant reduction in umbilical cord separation time due to topic after the application al application of breastmilk on umbilical cord. Hence, topical application of breastmilk can be used as a cheapest, easily available natural therapy for reduction of umbilical cord separation time.

Triangulum (M33) is the most massive satellite galaxy of Andromeda (M31), with a stellar mass of about 3 × 109 M⊙. Based on abundance matching techniques, M33’s total mass at infall is estimated to be of order 1011M⊙. ΛCDM simulations predict that M33-mass halos host several of their own low-mass satellite companions, yet only one candidate M33 satellite has been detected in deep photometric surveys to date. This ‘satellites of satellites’ hierarchy has recently been explored in the context of the dwarf galaxies discovered around the Milky Way’s Magellanic Clouds in the Dark Energy Survey. Here, we discuss the number of satellite galaxies predicted to reside within the virial radius (∼160 kpc) of M33 based on ΛCDM simulations. We also calculate the expected number of satellite detections in N fields of data using various ground-based imagers. Finally, we discuss how these satellite population predictions change as a function of M33’s past orbital history. If M33 is on its first infall into M31’s halo, its proposed satellites are expected to remain bound to M33 today. However, if M33 experienced a recent tidal interaction with M31, the number of surviving satellites depends strongly on the distance achieved at pericenter due to the effects of tidal stripping. We conclude that a survey extending to ∼100 kpc around M33 would be sufficient to constrain its orbital history and a majority of its satellite population. In the era of WFIRST, surveys of this size will be considered small observing programs.

Despite recent improvements to in situ proteolysis strategies, a higher efficiency is still needed to increase both the number of peptides detected and the associated ion intensity, leading to a complete and reliable set of biomarkers for diagnostic or prognostic purposes. In the study presented here, an extract of a systematic study is illustrated investigating a range of surfactants assisting trypsin proteolytic activity. Method development was trialled on fingermarks; this specimen results from a transfer of sweat from an individual’s fingertip to a surface upon contact. As sweat carries a plethora of biomolecules, including peptides and proteins, fingermarks are, potentially, a very valuable specimen for non-invasive prognostic or diagnostic screening. A recent study has demonstrated the opportunity to quickly detect peptides and small proteins in fingermarks using Matrix Assisted Laser Desorption Ionization Mass Spectrometry Profiling (MALDI MSP). However, intact detection bears low sensitivity and does not allow species identification; therefore, a shotgun proteomic approach was employed involving in situ proteolysis. Data demonstrate that in fingermarks, further improvements to the existing method can be achieved using MEGA-8 as surfactant in higher percentages as well as combinations of different detergents. Also, for the first time, Rapigest SF, normally used in solution digestions, has been shown to successfully work also for in situ proteolysis. Graphical Abstract ᅟ Electronic supplementary material The online version of this article (doi:10.1007/s13361-015-1140-z) contains supplementary material, which is available to authorized users.

Dendritic cells (DCs) are important innate and adaptive immune effectors, and have a key role in antigen presentation and T-cell activation. Different lineages of DCs can be developed from hematopoietic progenitors following cytokine signaling, and the various lineages of DCs display distinct morphology, phenotype and functions. There has been limited information on differential cytokine-mediated molecular signaling in DCs. Analyses of surface molecules by flow cytometry and quantitative RNA profiling revealed differences between DCs derived from interleukin-4 (IL-4) versus IL-15 signaling, yet both lineages of DCs exhibited similar levels of surface molecules key to immune activation. Functional assays confirmed that IL-15-derived DCs elicited greater antigen-specific, primary and secondary CD8 and CD4 T-cell responses than did IL-4-derived DCs. Importantly, IL-15 DCs secreted substantial amounts of proinflammatory cytokines, including IL-6, interferon-γ (IFN-γ) and tumor necrosis factor-α (TNFα), which helped polarize a strong T-cell response. Assessment of signaling pathways revealed that IL-15 DCs exhibited a lower levels of activated signal transducer and activator of transcription 5 (STAT5), STAT6 and extracellular signal-regulated kinase 1/2 than IL-4 DCs, but after lipopolysaccharide (LPS)/TNFα treatment, the STAT3 and p38 mitogen-activated protein kinase (MAPK) activities were significantly enhanced in the IL-15 DCs. Surprisingly, contrary to the canonical IL-15-mediated STAT5 signaling pathway in lymphoid cells, IL-15 did not mediate a strong STAT5 or STAT3 activation in DCs. Further analysis using specific inhibitors to STAT3 and p38 MAPK pathways revealed that the STAT3 signaling, but not p38 MAPK signaling, contributed to IFN-γ production in DCs. Therefore, while IL-15 does not promote the STAT signaling in DCs, the increased STAT3 activity after LPS/TNFα treatment of the IL-15 DCs has a key role in their high IFN-γ effector activities.

Pharmacodynamics and toxicodynamics are the study of the biochemical and physiological effects of therapeutic agents and toxicants and their mechanisms of action. MALDI-MS imaging offers great potential for the study of pharmaco/toxicodynamic responses in tissue owing is its ability to study multiple biomarkers simultaneously in a label-free manner. Here, existing examples of such studies examining anticancer drugs and topically applied treatments are described. Examination of the literature shows that the use of MS imaging in pharmaco/toxicodynamic studies is in fact quite low. The reasons for this are discussed and potential developments in the methodology that might lead to its further use are described.

In this paper, the concepts, design and charged particles simulation for developing a new multi-plates Velocity-Map Imaging (VMI) spectrometer are discussed. This spectrometer very is powerful for 3D velocity vector imaging of charged particles. The standard design of Eppink and Parker [Rev. Sci. Instrum. 68, 3477 (1997)] velocity map imaging has three-plates which under suitable voltage at each plates make a “asymmetric immersion electrostatic lens”. This lens mapped the same initial velocity vector at one point on the detector. The three-plate VMI setup is useful for 3D imaging of low energy electrons (

We present new proper motion (PM) measurements of the dwarf spheroidal galaxies (dSphs) Draco and Sculptor using multi-epoch images obtained with the Hubble Space Telescope ACS/WFC. Our PM results have uncertainties far lower than previous measurements, even made with the same instrument. The PM results for Draco and Sculptor are (mu_W,mu_N)_Dra = (-0.0562+/-0.0099,-0.1765+/-0.0100) mas/yr and (mu_W,mu_N)_Scl = (-0.0296+/-0.0209,-0.1358 +/-0.0214) mas/yr. The implied Galactocentric velocity vectors for Draco and Sculptor have radial and tangential components: (V_rad,V_tan)_Dra = (-88.6,161.4) +/- (4.4,5.6) km/s; and (V_rad,V_tan)_Scl = (72.6,200.2) +/- (1.3,10.8) km/s. We study the detailed orbital history of both Draco and Sculptor via numerical orbit integrations. Orbital periods of Draco and Sculptor are found to be 1-2 and 2-5 Gyrs, respectively, accounting for uncertainties in the MW mass. We also study the influence of the Large Magellanic Cloud (LMC) on the orbits of Draco and Sculptor. Overall, the inclusion of the LMC increases the scatter in the orbital results. Based on our calculations, Draco shows a rather wide range of orbital parameters depending on the MW mass and inclusion/exclusion of the LMC, but Sculptor's orbit is very well constrained with its most recent pericentric approach to the MW being 0.3-0.4 Gyr ago. Our new PMs imply that the orbital trajectories of both Draco and Sculptor are confined within the Disk of Satellites (DoS), better so than implied by earlier PM measurements, and likely rule out the possibility that these two galaxies were accreted together as part of a tightly bound group., 17 pages, 8 figures, 6 tables. Accepted for publication in ApJ

In Matrix Assisted Laser Desorption Ionization Mass Spectrometry Imaging (MALDI-MSI), identification of observed proteins remains a challenge primarily due to the drop in sensitivity of time-of-flight (TOF) mass spectrometers beyond the mass range of 25-35 kDa (Francese et al. High Throughput Screen 12: 156-174, 2009) and inadequate mass resolving power at those molecular weights. To counteract this, a bottom-up proteomic approach is often employed. Proteolysis digests proteins into smaller peptide fragments, typically between 500 and 3000 Da which are easier to detect with a higher mass accuracy. Here, we describe the addition of a MS-compatible detergent to the endopeptidase trypsin, to enhance in situ proteolysis efficiency and peptide intensity.

Matrix-Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry Imaging (MSI) is fast becoming an industry leading technique as a means of investigating the distributions of protein and peptide molecules directly from sections of tissue. Developing protocols for the analysis of FFPE tissue opens up numerous opportunities for novel biomarker discovery, due to the large number of tissue banks containing FFPE biopsy samples. This chapter reports the analysis of both fresh frozen and formalin-fixed paraffin-embedded (FFPE) tissues using such protocols.

Multimedia is an important part of todays visual world. The raw vidos and images are of large size. To store them on disk or transmit over the network it is difficult Due to large size of raw image and videos compression is apply to reduce size. for compression H.264 video coading standard is applied. during compression each frame is divided into macroblocks can lead to visible quality losses known as blocking artifacts. To improve the visual quality of video deblocking filter is applied to reconstructed frame. Considering that deblocking filter is applied to all of the vertical and horizontal edges of blocks but only some of them actually need to be filtered, in this paper, based on previous encoding or decoding information, we propose a novel deblocking filter algorithm to reduce complexity of exist algorithms by skipping quantities of needless edges. These experimental results show that our proposed algorithm has better performance in subjective video quality and time efficiency.

In Matrix Assisted Laser Desorption Ionization Mass Spectrometry Imaging (MALDI-MSI), identification of observed proteins remains a challenge primarily due to the drop in sensitivity of time-of-flight (TOF) mass spectrometers beyond the mass range of 25-35 kDa (Francese et al. High Throughput Screen 12: 156-174, 2009) and inadequate mass resolving power at those molecular weights. To counteract this, a bottom-up proteomic approach is often employed. Proteolysis digests proteins into smaller peptide fragments, typically between 500 and 3000 Da which are easier to detect with a higher mass accuracy. Here, we describe the addition of a MS-compatible detergent to the endopeptidase trypsin, to enhance in situ proteolysis efficiency and peptide intensity.

Matrix-Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry Imaging (MSI) is fast becoming an industry leading technique as a means of investigating the distributions of protein and peptide molecules directly from sections of tissue. Developing protocols for the analysis of FFPE tissue opens up numerous opportunities for novel biomarker discovery, due to the large number of tissue banks containing FFPE biopsy samples. This chapter reports the analysis of both fresh frozen and formalin-fixed paraffin-embedded (FFPE) tissues using such protocols.

Aerococcus species were first described in 1953. They have been previously described as the causative agent of urinary tract infections in the elderly but rarely the causative agent for severe infections. However, there are very few cases reported in the pediatric population. To our knowledge, we report the first case of an adolescent with infective endocarditis caused by Aerococcus urinae.

The 3D velocities of M31 and M33 are important for understanding the evolution and cosmological context of the Local Group. Their most massive stars are detected by Gaia, and we use Data Release 2 (DR2) to determine the galaxy proper motions (PMs). We select galaxy members based on, e.g., parallax, PM, color-magnitude-diagram location, and local stellar density. The PM rotation of both galaxies is confidently detected, consistent with the known line-of-sight rotation curves: $V_{\rm rot} = -206\pm86$ km s$^{-1}$ (counter-clockwise) for M31, and $V_{\rm rot} = 80\pm52$ km s$^{-1}$ (clockwise) for M33. We measure the center-of-mass PM of each galaxy relative to surrounding background quasars in DR2. This yields that $({\mu}_{\alpha*},{\mu}_{\delta})$ equals $(65 \pm 18 , -57 \pm 15)$ $\mu$as yr$^{-1}$ for M31, and $(31 \pm 19 , -29 \pm 16)$ $\mu$as yr$^{-1}$ for M33. In addition to the listed random errors, each component has an additional residual systematic error of 16 $\mu$as yr$^{-1}$. These results are consistent at 0.8$\sigma$ and 1.0$\sigma$ with the (2 and 3 times higher-accuracy) measurements already available from Hubble Space Telescope (HST) optical imaging and VLBA water maser observations, respectively. This lends confidence that all these measurements are robust. The new results imply that the M31 orbit towards the Milky Way is somewhat less radial than previously inferred, $V_{\rm tan, DR2+HST} = 57^{+35}_{-31}$ km s$^{-1}$, and strengthen arguments that M33 may be on its first infall into M31. The results highlight the future potential of Gaia for PM studies beyond the Milky Way satellite system., Comment: 15 pages, 7 figures, ApJ, in press

In this paper, we present a semi real-time vehicle tracking algorithm to determine the speed of the vehicles in traffic from traffic cam video. The results of this work can be used for traffic control, security and safety both by government agencies and commercial organizations. In this paper a method is described for tracking moving objects from a sequence of video frame. This method is implemented by using optical flow (Horn-Schunck)and (Lucas-Kanade) in mat lab and Simulink. It has a variety of uses, some of which are: human computer interaction, security and surveillance, video communication and compression, augmented reality, traffic control, medical imaging and video editing. Segmentation is performed to detect the object after reducing the noise from that scene. The object is tracked by plotting a rectangular bounding box around it in each frame. The velocity of the object is determined by calculating the distance that the object moved in a sequence of frames with respect to the frame rate that the video is recorded. Comparison and performance analysis of algorithms based on psnr and average angular error is done.

The Milky Way (MW) and M31 both harbor massive satellite galaxies, the Large Magellanic Cloud (LMC) and M33, which may comprise up to 10 per cent of their host's total mass. Massive satellites can change the orbital barycentre of the host-satellite system by tens of kiloparsecs and are cosmologically expected to harbor dwarf satellite galaxies of their own. Assessing the impact of these effects depends crucially on the orbital histories of the LMC and M33. Here, we revisit the dynamics of the MW-LMC system and present the first detailed analysis of the M31-M33 system utilizing high precision proper motions and statistics from the dark matter-only Illustris cosmological simulation. With the latest Hubble Space Telescope proper motion measurements of M31, we reliably constrain M33's interaction history with its host. In particular, like the LMC, M33 is either on its first passage (t_{inf} < 2 Gyr ago) or if M31 is massive (>=2x10^12 Msun), it is on a long period orbit of about 6 Gyr. Cosmological analogs of the LMC and M33 identified in Illustris support this picture and provide further insight about their host masses. We conclude that, cosmologically, massive satellites like the LMC and M33 are likely completing their first orbits about their hosts. We also find that the orbital energies of such analogs prefer a MW halo mass ~1.5x10^12 Msun and an M31 halo mass >=1.5x10^12 Msun. Despite conventional wisdom, we conclude it is highly improbable that M33 made a close (< 100 kpc) approach to M31 recently ( t_{peri} < 3 Gyr ago). Such orbits are rare (< 1 per cent) within the 4$��$ error space allowed by observations. This conclusion cannot be explained by perturbative effects through four body encounters between the MW, M31, M33, and the LMC. This surprising result implies that we must search for a new explanation for M33's strongly warped gas and stellar discs., 28 pages, 17 figures, 8 tables, accepted to MNRAS Oct 7 2016

Objectives This study sought to measure the impact of pre-hospital (PH) electrocardiography (ECG) on scene-to-hospital time for patients with chest pain of cardiac origin and those with ST-segment elevation myocardial infarction (STEMI). Background Pre-hospital ECG decreases door-to balloon (D2B) time for STEMI patients. However, obtaining a PH ECG might prolong scene time. We investigated the impact of obtaining a PH ECG on both scene and transport times for patients with chest pain suspected of cardiac origin. Methods City of San Diego Emergency Medical System runsheets of patients with chest pain from January 2003 to April 2008 were analyzed. The scene times and transport times were compared before (from January 2003 to December 2005) and after (from January 2006 to April 2008) implementation of the PH ECG. Among patients with a PH ECG, median scene times and transport times were compared in patients with and without STEMI. Results There were 21,742 patients evaluated for chest pain during the study period. Implementation of PH ECG resulted in minimal increases in median scene time (19 min, 10 s vs. 19 min, 28 s, p = 0.002) and transport time (13 min, 16 s vs. 13 min, 28 s, p = 0.007). However, compared with chest pain patients, in STEMI patients (n = 303), shorter median scene time (17 min, 51 s vs. 19 min, 31 s, p Conclusions Obtaining a PH ECG for patients with chest pain minimally prolongs scene and transport times. Further, for STEMI patients, both scene times and transport times are actually reduced leading to a potential reduction in total ischemic time.

Recent studies have described a gender bias against women in the setting of acute coronary syndrome (ACS).We sought to measure the impact that a prehospital electrocardiogram (PH ECG) has on prehospital total scene time to hospital arrival time, comparing men and women with the complaint of chest pain (cCP).This study retrospectively analyzed San Diego Emergency Medical Services (EMS) runsheets of patients with cCP before and after implementation of the PH ECG protocol. The average scene time (ST), transport time (TT), and total scene-to-arrival-at-hospital time (STH) were compared. After stratification by gender, times were compared in patients with ST-elevation myocardial infarction (STEMI) to those without STEMI.Of 21,742 EMS activations for patients with cCP, there were no significant differences overall. When stratified by gender, there was a significant reduction of ST (00:19:16 min vs. 00:20:48 min, p0.001, 95% CI 00:01:17-00:01:48) and STH (00:33:22 min vs. 00:35:44 min, p0.001, 95% CI 00:01:21-00:02:24) favoring men in cases without STEMI. In cases of STEMI, men had a significant reduction in ST (00:17:27 min vs. 00:20:29 min, p0.001, 95% CI 00:01:24-00:04:40) and STH (00:30:30 min vs. 00:34:25 min, p0.01, 95% CI 00:01:23-00:06:26) times compared to women.Prehospital ECG implementation led to no significant differences in pre- and post-implementation times. In cases of STEMI, men had significantly reduced scene time and scene-to-hospital time when compared to women. The precise reason for these disparities remains unknown.

This paper presents a novel velocity estimation method for ground vehicles. The task here is to automatically estimate vehicle speed from video sequences acquired with a fixed mounted camera. The vehicle motion is detected and tracked along the frames using Lucas-Kanade algorithm. The distance traveled by the vehicle is calculated using the movement of the centroid over the frames and the speed of the vehicle is estimated. The average speed of cars is determined from various frames. The application is developed using MATLAB and SIMULINK.

The current Infection and Treatment Method of vaccination against East Coast fever comprises an inoculation of live Theileria parva sporozoites and simultaneous administration of oxytetracycline. Immunization with a combination of parasite types has been shown to provide broader protection than inoculation of individual strains. In this study, we used a high-throughput capillary electrophoresis system to determine the genotypic composition of the Muguga Cocktail, a widely used vaccine stabilate derived from three seed stabilates-Muguga, Serengeti-transformed and Kiambu 5. Five satellite markers were used to genotype the vaccine and reference stabilates from two commercial-scale preparations of the vaccine. In addition, 224 cloned cell lines established by infection of bovine lymphocytes with T. parva parasites from the component stabilates were genotyped. The results indicate that, for the recently prepared batch, there are at least eight genotypes in each of the Muguga and the Serengeti-transformed stabilates, while parasites from the Kiambu 5 stabilate showed no diversity at the five loci. The Serengeti-transformed stabilate contained parasites of the Kiambu 5 genotype and of two genotypes present in the Muguga stabilate, whereas there were no genotypes common to the Muguga and Kiambu 5 stabilates. When stabilates from the two vaccine batches were compared, no allelic variations were identified between the Muguga and Kiambu 5 parasites, while lack of sufficient clones prevented a full comparison of the Serengeti-transformed stabilates. The findings will facilitate examination of the extent to which the vaccine strains become resident in areas under vaccination, the identification of 'breakthrough' strains and the establishment of the quality assurance protocols to detect variations in the production of the vaccine. The cloned cell lines will be useful for further understanding the antigenic diversity of parasites in the vaccine.

Despite success in treating hematological malignancies, T cells expressing chimeric antigen receptors (CARs) have shown poor efficacy in solid tumor indications. The poor utility of CARs is thought to be due to a number of factors including T cell exhaustion and a lack of persistence. Utilizing only the CD3ζ chain of six distinct T cell receptor subunits in combination with a costimulatory domain, the CARs bypass the natural TCR signaling complex. The failure to initiate and harness a complete TCR response is arguably a primary underlying factor preventing CAR-T cell success in solid tumor indications. Here, we present a novel T cell engineering platform: T Cell Receptor Fusion Constructs (TRuC™s), which target tumors in a MHC non-restricted fashion. Unlike CARs, the constructs integrate into the TCR complex, harnessing the full potential of natural T cell activation, effector function and regulation. Here we demonstrate preclinical evidence underscoring the efficacy of TRuC™-T cells re-programmed to target the solid tumor antigen mesothelin (TC-210). TC-210 showed robust anti-tumor activity in vitro and in vivo in mesothelioma, lung and ovarian tumor models. Surprisingly, this activity was associated with less proinflammatory cytokine production compared to CAR-T cells with the same binder and was supported by strong TRuC™-T cell persistence. Taken together, mesothelin targeting TRuC™-T cells have potent anti-tumor activity and present a promising approach for treating mesothelin-expressing solid tumors. Citation Format: Jian Ding, Ekta Patel, Patrick Tavares, Justin Quinn, Rashmi Choudhary, Bonnie Le, Olga Kiner, Solly Weiler, Daniel R. Getts, Patrick Baeuerle, Robert Hofmeister. Preclinical evaluation of mesothelin-specific T cell receptor (TCR) fusion constructs (TRuC™s) utilizing the signaling power of the complete TCR complex: A new opportunity for solid tumor therapy [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 3589.

T cells expressing chimeric antigen receptors (CARs) have demonstrated remarkable clinical benefit in certain hematological malignancies albeit with side effect such as cytokine release syndrome, on Target off tumor response and limited efficacy in solid tumors. Here, we present the preclinical evaluation of a novel T cell engineering platform designed to overcome potential hurdles of CARs and other T cell receptor modalities. Specifically, we have generated novel T Cell Receptor Fusion Constructs (TRuC™s) that fuse binder domains to subunits of the T cell receptor. These constructs when introduced using lentiviral technology, integrate into endogenous TCR, reprograming T cells to target tumor antigen in a non-major histocompatibility complex (MHC) restricted fashion and harnessing the full spectrum of TCR signaling. TRuC™ variants were constructed by recombinant fusion of an scFv or sdAb to various TCR subunits via a flexible linker sequence. Likewise, CD28ζ and 41BBζ CARs were generated using the same binders for side-by-side comparison. We demonstrated that TRuC™ variants can effectively reprogram T cells to recognize tumor surface antigens in a non-MHC-restricted fashion. TRuC™s are distinct from CARs in their ability to activate T cells through the entire TCR without additional costimulatory domains. In vitro, TRuC™ T cells were equally potent as CAR T cells in eliminating tumor cells. Compared to CAR-T cells, TRuC-T cells produced lower cytokine levels and proliferated less. Despite these difference, TRuC™ T cells were more efficient in clearing tumors in subcutaneous Raji and MSTO-211H mesothelioma models. Our findings support the development of TRuC-T cells for the treatment of solid tumors. Citation Format: Ekta Patel, Jian Ding, Nikolaus Thorausch, Janani Krishnamurthy, Rashmi Choudhary, Solly Weiler, Bonnie Le, Patrick Tavares, Adam Zieba, Justin Quinn, Yan Wang, Wolfgang Schamel, Irene Scarfo, Marcela Maus, Patrick Baeuerle, Dainel Getts, Robert Hofmeister. Characterization of a novel class of engineered (TCR) fusion constructs (TRuCTMs) aimed to treat solid tumors [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 3584.