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51. Earl May's 1933 spring catalog /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Berries, Catalogs, Department stores, Equipment and supplies, Evergreens, Field crops, Flowers, Fruit trees, Grasses, Hardware stores, Iowa, Nurseries (Horticulture), Nursery stock, Plants, Ornamental, Poultry, Seedlings, Seeds, Shenandoah, Shrubs, Trees, Vegetables
Published
1933

52. Earl May's 1940 nursery and seed catalog, 20th Anniversary /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Fruit trees, Iowa, Nursery stock, Plants, Ornamental, Seedlings, Seeds, Shenandoah
Published
1940

53. Earl May's 1932 spring catalog /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Department stores, Equipment and supplies, Flowers, Hardware stores, Iowa, Nurseries (Horticulture), Nursery stock, Poultry, Seeds, Shenandoah
Published
1932

54. Nursery stock prices /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Earl May Seed & Nursery Co, Iowa, Nursery stock, Prices, Shenandoah
Published
1925

55. Earl May's lowest farm seed prices : effective January 1, 1931 /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Field crops, Iowa, Nurseries (Horticulture), Nursery stock, Prices, Seeds, Shenandoah, Vegetables
Published
1931

56. Earl May's 1931 spring catalog /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Department stores, Equipment and supplies, Flowers, Hardware stores, Iowa, Nurseries (Horticulture), Nursery stock, Poultry, Seeds, Shenandoah
Published
1931

57. May's wholesale farm seed prices /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Field crops, Iowa, Nursery stock, Prices, Seed industry and trade, Seeds, Shenandoah, Wholesale trade
Published
1930

58. Earl May's 1930 spring catalog /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Berries, Bulbs (Plants), Catalogs, Department stores, Equipment and supplies, Field crops, Flowers, Fruit trees, Hardware stores, Iowa, Nurseries (Horticulture), Nursery stock, Plants, Ornamental, Poultry, Seed industry and trade, Seedlings, Seeds, Shenandoah, Shrubs, Trees, Vegetables
Published
1930

59. Earl May's 1930 fall nursery & seed catalog /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Bulbs (Plants), Catalogs, Department stores, Hardware stores, Iowa, Nurseries (Horticulture), Nursery stock, Plants, Ornamental, Shenandoah
Published
1930

60. Earl E. May's advance sale : fall & winter 1929 /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Department stores, Hardware stores, Iowa, Nursery stock, Shenandoah
Published
1929

61. May's wholesale prices on farm seeds /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Field crops, Iowa, Nursery stock, Prices, Seeds, Shenandoah, Wholesale trade
Published
1929

62. Earl May's midseason sale : summer 1929 /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Department stores, Hardware stores, Iowa, Nursery stock, Shenandoah
Published
1929

63. Earl E. May Seed & Nursery Co., 1929 /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Agricultural implements, Catalogs, Equipment and supplies, Field crops, Flowers, Fruit trees, Iowa, Nursery stock, Plants, Ornamental, Poultry, Seedlings, Seeds, Shenandoah, Trees, Vegetables
Published
1929

64. [Catalog].

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Iowa, Nursery stock, Prices, Seeds, Shenandoah
Published
1928

65. May's big fall catalog : 1928 bulbs & merchandise /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Agricultural implements, Catalogs, Commercial catalogs, Hardware, Iowa, Nurseries (Horticulture), Nursery stock, Shenandoah
Published
1928

66. May Seed & Nursery Co. [catalog] /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Agricultural implements, Berries, Catalogs, Earl May Seed & Nursery Co, Equipment and supplies, Flowers, Fruit trees, Grasses, Iowa, Nursery stock, Plants, Ornamental, Poultry, Seedlings, Seeds, Shenandoah, Shrubs, Trees, Vegetables
Published
1927

67. Season of 1926 [catalog] /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Agricultural implements, Berries, Catalogs, Earl May Seed & Nursery Co, Fruit trees, Grasses, Iowa, Nursery stock, Plants, Ornamental, Prices, Seedlings, Seeds, Shenandoah, Vegetables
Published
1926

68. May's hardy perennials /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Berries, Catalogs, Earl May Seed & Nursery Co, Fruit trees, Grasses, Iowa, Nursery stock, Perennials, Plants, Ornamental, Prices, Seedlings, Seeds, Shenandoah, Vegetables
Published
1925

69. May's 1924 condensed catalog : March 8th, 1924 /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Berries, Catalogs, Earl May Seed & Nursery Co, Fruit trees, Grasses, Iowa, Nursery stock, Plants, Ornamental, Prices, Seedlings, Seeds, Shenandoah, Vegetables
Published
1924

70. Spring 1924 [catalog] /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Agricultural implements, Berries, Catalogs, Earl May Seed & Nursery Co, Fruit trees, Grasses, Iowa, Nursery stock, Plants, Ornamental, Prices, Seedlings, Seeds, Shenandoah, Vegetables
Published
1924

71. May's views : February 1923 /

Author

Earl May Seed & Nursery Co, May, Earl E., Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl E., and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Agricultural implements, Catalogs, Earl May Seed & Nursery Co, Fruit, Grasses, Iowa, Nursery stock, Prices, Seeds, Shenandoah
Published
1923

72. May's seed book : spring 1923 /

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Agricultural implements, Berries, Catalogs, Earl May Seed & Nursery Co, Flowers, Fruit trees, Grasses, Iowa, Nursery stock, Prices, Seedlings, Seeds, Shenandoah, Vegetables
Published
1923

73. May's 45th annual catalog of Northern grown seeds and nursery products /

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Berries, Catalogs, Field crops, Fruit trees, Iowa, Nursery stock, Plants, Ornamental, Seedlings, Seeds, Shenandoah, Shrubs, Trees
Published
1925

74. May's extra value bulletin : February 15, 1922 /

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Agricultural implements, Berries, Catalogs, Earl May Seed & Nursery Co, Field crops, Fruit trees, Grasses, Iowa, Nursery stock, Prices, Seedlings, Seeds, Shenandoah, Vegetables
Published
1922

75. May's seed book : spring 1922 /

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Agricultural implements, Berries, Catalogs, Earl May Seed & Nursery Co, Field crops, Fruit trees, Grasses, Iowa, Nursery stock, Seedlings, Seeds, Shenandoah, Vegetables
Published
1922

76. A descriptive catalog of general stock /

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Agricultural implements, Berries, Catalogs, Earl May Seed & Nursery Co, Field crops, Fruit trees, Grasses, Iowa, Nursery stock, Seedlings, Seeds, Shenandoah, Vegetables
Published
1922

77. May's bulletin /

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Agricultural implements, Catalogs, Grasses, Iowa, Nursery stock, Seeds, Shenandoah, Vegetables
Published
1921

78. May Seed and Nursery Co. [catalog].

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Fruit, Fruit trees, Iowa, Nursery stock, Plants, Ornamental, Seedlings, Shenandoah, Shrubs, Trees
Published
1921

79. Spring 1921 May's annual seed book : multiply America's yield /

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Agricultural implements, Catalogs, Flowers, Fruit, Grasses, Iowa, Nursery stock, Plants, Ornamental, Seeds, Shenandoah, Vegetables
Published
1921

80. 1959 Earl May nursery & seed catalog

Author

Earl May Seed & Nursery Co, May, Earl, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, May, Earl, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Fruit trees, Iowa, Nursery stock, Plants, Ornamental, Seedlings, Seeds, Shenandoah, Varieties, Vegetables

81. 1958 Earl May nursery and seed catalog

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Fruit trees, Iowa, Nursery stock, Plants, Ornamental, Seedlings, Seeds, Shenandoah, Varieties, Vegetables

82. Imported tulips and other spring flowering bulbs : fall 1957

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Bulbs (Plants), Catalogs, Iowa, Nursery stock, Plants, Ornamental, Shenandoah, Varieties

83. Earl May 1957 nursery and seed catalog

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Fruit trees, Iowa, Nursery stock, Plants, Ornamental, Seedlings, Seeds, Shenandoah, Varieties, Vegetables

84. Earl May nursery and seed catalog spring 1956

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Fruit trees, Iowa, Nursery stock, Plants, Ornamental, Seedlings, Seeds, Shenandoah, Varieties, Vegetables

85. Earl May's 1948 fall catalog

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Bulbs (Plants), Catalogs, Iowa, Nursery stock, Perennials, Shade trees, Shenandoah, Varieties

86. Imported direct from Holland, fall 1955

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Bulbs (Plants), Catalogs, Iowa, Nursery stock, Plants, Ornamental, Shenandoah, Varieties

87. Earl May nursery and seed catalog spring 1955

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Fruit trees, Iowa, Nursery stock, Plants, Ornamental, Seedlings, Seeds, Shenandoah, Varieties, Vegetables

88. Summer and fall 1955

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Iowa, Irises (Plants), Nursery stock, Shenandoah, Varieties

89. Earl May nursery and seed spring 1954 catalog

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Fruit trees, Iowa, Nursery stock, Plants, Ornamental, Seedlings, Seeds, Shenandoah, Varieties, Vegetables

90. 1954 fall catalog

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Bulbs (Plants), Catalogs, Iowa, Nursery stock, Perennials, Shenandoah, Varieties

91. Maygold

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Corn, Iowa, Nursery stock, Shenandoah, Varieties

92. May's mountain grown iris for 1954

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Iowa, Irises (Plants), Nursery stock, Shenandoah, Varieties

93. The Earl May 1953 nursery and seed catalog

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Fruit trees, Iowa, Nursery stock, Plants, Ornamental, Seedlings, Seeds, Shenandoah, Varieties, Vegetables

94. Earl May's 1952 fall catalog

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Bulbs (Plants), Catalogs, Iowa, Nursery stock, Perennials, Shenandoah, Varieties

95. The Earl May 1952 nursery and seed catalog

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Fruit trees, Iowa, Nursery stock, Plants, Ornamental, Seedlings, Seeds, Shenandoah, Trees, Varieties, Vegetables

96. The Earl May 1951 nursery and seed catalog

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Fruit trees, Iowa, Nursery stock, Plants, Ornamental, Seedlings, Seeds, Shenandoah, Trees, Varieties, Vegetables

97. Earl May 1950 fall catalog

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Bulbs (Plants), Catalogs, Iowa, Irises (Plants), Nursery stock, Perennials, Plants, Ornamental, Shenandoah, Tulips, Varieties

98. Earl May 1949 fall catalog

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Bulbs (Plants), Catalogs, Iowa, Nursery stock, Perennials, Shenandoah, Tulips, Varieties

99. Earl May's 1948 nursery and seed catalog

Author

Earl May Seed & Nursery Co, Henry G. Gilbert Nursery and Seed Trade Catalog Collection, U.S. Department of Agriculture, National Agricultural Library, Earl May Seed & Nursery Co, and Henry G. Gilbert Nursery and Seed Trade Catalog Collection

Subjects
Catalogs, Iowa, Nursery stock, Plants, Ornamental, Seeds, Shenandoah

100. An intrinsic ATPase activity of phospho-MEK-1 uncoupled from downstream ERK phosphorylation

Author

Timothy Broderick, Luke H. Carter, Michael D. Schaber, Robert A. Copeland, Earl May, Richard R. Gontarek, Cynthia M. Rominger, Kurt Weaver, and Jingsong Yang

Subjects
Proto-Oncogene Proteins B-raf, MAPK/ERK pathway, Time Factors, MAP Kinase Kinase 1, Biophysics, Biochemistry, Phosphates, chemistry.chemical_compound, Adenosine Triphosphate, ATP hydrolysis, Lactate dehydrogenase, Nitriles, Butadienes, Escherichia coli, Animals, Humans, Phosphorylation, Extracellular Signal-Regulated MAP Kinases, Molecular Biology, Adenosine Triphosphatases, chemistry.chemical_classification, Kinase, Hydrolysis, Cell biology, Kinetics, Enzyme, chemistry, Rabbits, Signal transduction, Pyruvate kinase, Signal Transduction
Abstract

We have developed a highly sensitive assay of MEK-mediated ATP hydrolysis by coupling the formation of ADP to NADH oxidation through the enzymes pyruvate kinase and lactate dehydrogenase. Robust ATP hydrolysis is catalyzed by phosphorylated MEK in the absence of the protein substrate ERK. This ERK-uncoupled ATPase activity is dependent on the phosphorylation status of MEK and is abrogated by the selective MEK kinase inhibitor U0126. ADP production is concomitant with Raf-mediated phosphorylation of MEK. Based on this finding, a coupled Raf/MEK assay is developed for measuring the Raf activity. A kinetic treatment derived under steady-state assumptions is presented for the analysis of the reaction progress curve generated by this coupled assay. We have shown that inhibitory potency of selective Raf inhibitors can be determined accurately by this assay.

Published
2007

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