Search

The change of the enzyme SDH activity in the liver as well as the change of the biochemical parameters (sGOT, sGPT and bilirubins) in the blood of the rats treated with gentamicin. In the experimental group of animals treated with gentamicin (100 mg/kg TT/24 h) for eight days it was found that the highest SDH activity was in the peri venular zone of the acinus (+++), that the enzyme's reduced activity was in the intermediary zone (+) and that the SDH activity in the per portal zone of the acinus was just in traces (+). In the control group of animals treated with the physiological solution (1 ml/kg TT/24 h), the enzyme SDH activity was the most prominent in the perivenular zone of the acinus (+++), while in the intermediary and the periportal zone the enzyme's activity was preserved but somewhat weaker (++). The results of the comparative study of enzymohistochemical and biochemical changes confirm the existence of an important correlation between the gentamicin application and the observed changes.

Exercise appears to increase reactive oxygen species (ROS), which can result in damage to cells. Potential sources of ROS in skeletal muscle fibers during exercise include the mitochondrial respiratory chain, xantine oxidase production of superoxide, enzymatic arachidonic acid oxygenation, nitric oxide synthesis, catecholamine oxidation, and neutrophil-induced oxidative burts. Stress proteins (SPs) represent one of the general molecular protective mechanisms that enable cell and whole organisms to survive stress. The exact relationship between exercise, ROS and SPs remains unclear. Antioxidant supplements have been touted by manufacturers as a means for athletes to perform better, recover more quickly and fully from endurance exercise, or allow them to train more strenuously. At present, data are insufficient to recommend antioxidant supplements for athletes or other persons who exercise regularly. However, no one questions the importance of ingesting a diet rich in antioxidants for all who exercise and train regularly.

Aminoglycoside antibiotics are obligated nephrotoxins and inevitably cause renal failure during prolonged use. Experimental models of gentamicin-induced nephrotoxicity have shown histopathological, ultrastructural and functional alteration with blood urea nitrogen and serum creatinine increase leading to acute renal insufficiency (ARI). The aim of our study was to emphasize effects of verapamil, a calcium channel blocker, on gentamicin-induced ARI in rats. Experiments were done on 50 male Wistar rats (250-300 g) divided in three experimental groups. G-group animals (20 rats) were treated daily with gentamicin in dose of 100 mg/kg during 8 days. GV-group animals (20 rats) were treated daily with verapamil in dose of 3 mg/kg and the same dose of gentamicin as in G-group during 8 days. The control group (10 rats) received 1 ml/day saline intraperitoneally. Histological examinations were done using hematoxylin and eosin, periodic acid Schiff and methenamine silver staining methods. Morphometric parameters included measurement of glomerular area, major and minor axis, perimeter, diameter, roundness, and mean optical density. Biochemical analyses were used to determine concentrations of blood urea, serum creatinine, sodium and potassium. In G-group rats’ glomerular basement membrane was diffusely and unequally thickened with polymorphonuclear neutrophils infiltration, while coagulation-type necrosis and vacuolization of cytoplasm of proximal tubules epithelial cells were observed. In GV-group rats’ glomeruli were slightly enlarged with thickened basement membrane in some segments but without coagulation-type necrosis. Morphometric analyses showed statistically significant differences between the G-group and control group of animals in glomerular size, mean optical density and average roundness (p

Gastrointestinal stromal tumors (GIST) represent the most usual mesenchymal tumors of the gastrointestinal tract. In this paper we present a case of a 59-year old patient with an adenocarcinoma of the transversal colon near the lienal flexure without breaking of the intestinal membrane synchronous with an intramural tumor in the antral region of the stomach which was not registered before the operation. Final immunohistochemical findings of the operation specimens: 1. Adenocarcinoma of the large intestine (histological grade I, nuclear grade I, Dukes A, invasion to submucosa) and 2. GIST with low malignant potential. Synchronous appearance of GISTs and colorectal adenocarcinoma, although very rare, has been increasingly observed in the last five years. Because of the small number of cases, a causal connection between these different types of tumors cannot be proven. Through this case report we hope to contribute to the adequate preoperative preparation, operation technique and postoperative monitoring of such patients. Surgical treatment is a dominant way of treating these synchronous tumors, so the surgeon has high responsibility for adequate selection of operative procedure on these patients.