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51. Quantification of glucose-6-phosphate dehydrogenase activity by spectrophotometry: A systematic review and meta-analysis

Author

Pfeffer, Daniel A., primary, Ley, Benedikt, additional, Howes, Rosalind E., additional, Adu, Patrick, additional, Alam, Mohammad Shafiul, additional, Bansil, Pooja, additional, Boum, Yap, additional, Brito, Marcelo, additional, Charoenkwan, Pimlak, additional, Clements, Archie, additional, Cui, Liwang, additional, Deng, Zeshuai, additional, Egesie, Ochaka Julie, additional, Espino, Fe Esperanza, additional, von Fricken, Michael E., additional, Hamid, Muzamil Mahdi Abdel, additional, He, Yongshu, additional, Henriques, Gisela, additional, Khan, Wasif Ali, additional, Khim, Nimol, additional, Kim, Saorin, additional, Lacerda, Marcus, additional, Lon, Chanthap, additional, Mekuria, Asrat Hailu, additional, Menard, Didier, additional, Monteiro, Wuelton, additional, Nosten, François, additional, Oo, Nwe Nwe, additional, Pal, Sampa, additional, Palasuwan, Duangdao, additional, Parikh, Sunil, additional, Pitaloka Pasaribu, Ayodhia, additional, Poespoprodjo, Jeanne Rini, additional, Price, David J., additional, Roca-Feltrer, Arantxa, additional, Roh, Michelle E., additional, Saunders, David L., additional, Spring, Michele D., additional, Sutanto, Inge, additional, Ley-Thriemer, Kamala, additional, Weppelmann, Thomas A., additional, von Seidlein, Lorenz, additional, Satyagraha, Ari Winasti, additional, Bancone, Germana, additional, Domingo, Gonzalo J., additional, and Price, Ric N., additional

Published
2020
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53. An experimental human blood stage model for studying Plasmodium malariae infection

Author

Woodford, John, Collins, Katharine A., Odedra, Anand, Wang, Claire, Kyung, Jang Ihn, Domingo, Gonzalo J., Watts, Rebecca, Marquart, Louise, Berriman, Matthew, Otto, Thomas D., and McCarthy, James S.

Subjects
parasitic diseases
Abstract

Background:\ud Plasmodium malariae is considered a ‘minor’ malaria parasite, although its global disease burden is underappreciated. The aim of this study was to develop an induced blood stage malaria (IBSM) model of P. malariae to study parasite biology, diagnostics, and treatment.\ud \ud Methods:\ud This clinical trial involved two healthy subjects who were intravenously inoculated with cryopreserved P. malariae-infected erythrocytes. Subjects were treated with artemether-lumefantrine following development of clinical symptoms. Prior to antimalarial therapy, mosquito feeding assays were performed to investigate transmission, and blood samples were collected for rapid diagnostic testing and parasite transcription profiling. Serial blood samples were collected for biomarker analysis.\ud \ud Results:\ud Both subjects experienced symptoms and signs typical of early malaria. Parasitaemia was detected 7 days post-inoculation and increased until antimalarial treatment was initiated 25 and 21 days post-inoculation for Subject 1 and 2 respectively (peak parasitaemia levels were 174,182 and 50,291 parasites/mL respectively). The parasite clearance half-life following artemether-lumefantrine treatment was 6.7 hours. Mosquito transmission was observed for one subject, while in vivo parasite transcription and biomarkers were successfully profiled.\ud \ud Conclusions:\ud An IBSM model of P. malariae has been successfully developed and may be used to study the biology, diagnostics, and treatment of this neglected malaria species.

Published
2019

57. Addressing the gender-knowledge gap in glucose-6-phosphate dehydrogenase deficiency: challenges and opportunities

Author

Domingo, Gonzalo J, Advani, Nicole, Satyagraha, Ari W, Sibley, Carol H, Rowley, Elizabeth, Kalnoky, Michael, Cohen, Jessica, Parker, Michael, and Kelley, Maureen

Subjects
Male, Health Knowledge, Attitudes, Practice, congenital, hereditary, and neonatal diseases and abnormalities, Gender, nutritional and metabolic diseases, Review, Primaquine, Chromosome, Antimalarials, Glucosephosphate Dehydrogenase Deficiency, Sex Factors, Risk Factors, Point-of-care, hemic and lymphatic diseases, Africa, parasitic diseases, Aminoquinolines, Malaria, Vivax, Humans, Female, X-inactivation, Glucose-6-phosphate dehydrogenase
Abstract

G6PD deficiency is a common X-linked genetic trait, with an associated enzyme deficiency phenotype, whereby males are either G6PD deficient or normal, but females exhibit a broader range of G6PD-deficiency, ranging from severe deficiency to normal. Heterozygous females typically have intermediate G6PD enzyme activity levels. In the malaria field, G6PD deficiency has significant implications for the safe and appropriate treatment for P.vivax malaria, as individuals with this deficiency are at greater risk of serious adverse events following treatment with the only curative class of anti-malarials, 8-aminoquinolines, such as primaquine. To date, the gold standard quantitative diagnostic tests for measuring G6PD deficiency are complex and require sophisticated laboratories. Qualitative tests, which are most commonly used, do not discriminate intermediate G6PD activity ranges. This has resulted in poor understanding of the epidemiology of G6PD activity in heterozygous females and its corresponding treatment ramifications. With the advent of simple-to-use quantitative tests for G6PD deficiency, and a momentum to eliminate malaria, there is an opportunity to address this knowledge gap. While this will require additional resources for clinical studies, adequate operational research, and appropriate pharmacovigilance, the health benefits from this investment go beyond the immediate intervention for which the G6PD status is first diagnosed.

Published
2018

59. Evaluation of a Novel Quantitative Test for Glucose-6-Phosphate Dehydrogenase Deficiency: Bringing Quantitative Testing for Glucose-6-Phosphate Dehydrogenase Deficiency Closer to the Patient

Author

Pal, Sampa, primary, Bansil, Pooja, additional, Bancone, Germana, additional, Hrutkay, Sevan, additional, Kahn, Maria, additional, Gornsawun, Gornpan, additional, Penpitchaporn, Pimsupah, additional, Chu, Cindy S., additional, Nosten, François, additional, and Domingo, Gonzalo J., additional

Published
2019
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60. Cytochemical flow analysis of intracellular G6PD and aggregate analysis of mosaic G6PD expression

Author

Kalnoky, Michael, Bancone, Germana, Kahn, Maria, Chu, Cindy S., Chowwiwat, Nongnud, Wilaisrisak, Pornpimon, Pal, Sampa, LaRue, Nicole, Leader, Brandon, Nosten, Francois, and Domingo, Gonzalo J.

Subjects
Male, congenital, hereditary, and neonatal diseases and abnormalities, Heterozygote, Erythrocytes, malaria, Primaquine, Glucosephosphate Dehydrogenase, Severity of Illness Index, lyonization, Antimalarials, Asian People, G6PD deficiency, hemic and lymphatic diseases, parasitic diseases, Humans, hemolytic anemia, Alleles, Hemizygote, Mosaicism, Contraindications, Drug, nutritional and metabolic diseases, Original Articles, Flow Cytometry, Thailand, United States, Black or African American, Glucosephosphate Dehydrogenase Deficiency, Gene Expression Regulation, Case-Control Studies, Mutation, Original Article, Female, Plasmodium vivax, Software
Abstract

Background Medicines that exert oxidative pressure on red blood cells (RBC) can cause severe hemolysis in patients with glucose-6-phosphate dehydrogenase (G6PD) deficiency. Due to X-chromosome inactivation, females heterozygous for G6PD with one allele encoding a G6PD deficient protein and the other a normal protein produce two RBC populations each expressing exclusively one allele. The G6PD mosaic is not captured with routine G6PD tests. Methods An open-source software tool for G6PD cytofluorometric data interpretation is described. The tool interprets data in terms of % bright RBC, or cells with normal G6PD activity in specimens collected from two geographically and ethnically distinct populations, an African-American cohort (US), and a Karen and Burman ethnic cohort (Thailand) comprising 242 specimens including 89 heterozygous females. Results The tool allowed comparison of data across two laboratories and both populations. Hemizygous normal or deficient males and homozygous normal or deficient females cluster at narrow % bright cells with mean values of 96%, or 6% (males) and 97%, or 2% (females) respectively. Heterozygous females, show a distribution of 10-85% bright cells, and a mean of 50%. The distributions are associated to severity of the G6PD mutation. Conclusions Consistent cytoflourometric G6PD analysis facilitates inter-laboratory comparison of cellular G6PD profiles and contributes to understanding primaquine associated hemolytic risk.

Published
2017

62. The G6PD flow-cytometric assay is a reliable tool for diagnosis of G6PD deficiency in women and anaemic subjects

Author

Bancone, Germana, Kalnoky, Michael, Chu, Cindy S., Chowwiwat, Nongnud, Kahn, Maria, Malleret, Benoit, Wilaisrisak, Pornpimon, Rénia, Laurent, Domingo, Gonzalo J., and Nosten, Francois

Subjects
Erythrocyte Indices, Male, Heterozygote, Genotype, lcsh:R, lcsh:Medicine, Anemia, Glucosephosphate Dehydrogenase, Flow Cytometry, Article, Hemoglobins, Glucosephosphate Dehydrogenase Deficiency, Phenotype, hemic and lymphatic diseases, parasitic diseases, Humans, lcsh:Q, Female, lcsh:Science
Abstract

Glucose-6-phosphate dehydrogenase (G6PD) activity is essential for redox equilibrium of red blood cells (RBCs) and, when compromised, the RBCs are more susceptible to haemolysis. 8-aminoquinolines (primaquine and tafenoquine) are used for the radical curative treatment of Plasmodium vivax malaria and can cause haemolysis in G6PD deficient subjects. Haemolytic risk is dependent on treatment dose and patient G6PD status but ultimately it correlates with the number of G6PD deficient RBCs. The G6PD spectrophotometric assay reliably identifies deficient subjects but is less reliable in heterozygous females, especially when other blood conditions are present. In this work we analysed samples with a range of G6PD phenotypes and haematologic conditions from 243 healthy volunteers of Asian or African-American heritage using both the spectrophotomeric assay and the G6PD flow-cytometric assay. Overall 18.5% of subjects (29.3% of Asian females) presented with anaemia, associated with decreased RBCs volume (MCV) and reticulocytosis; the flow-cytometric assay showed good correlation with the spectrophotometric assay (Pearson’s r 0.918–0.957) and was less influenced by haemoglobin concentration, number of RBCs and number of reticulocytes. This resulted in more precise quantification of the number of G6PD deficient RBCs and presumably higher predictive power of drug induced haemolytic risk.

Published
2017

63. Multiplex, DNase-free one-step reverse transcription PCR for Plasmodium 18S rRNA and spliced gametocyte-specific mRNAs

Author

Hanron, Amelia E, Billman, Zachary P, Seilie, Annette M, Olsen, Tayla M, Fishbaugher, Matthew, Chang, Ming, Rueckle, Thomas, Andenmatten, Nicole, Greenhouse, Bryan, Arinaitwe, Emmanuel, Rek, John, Das, Smita, Domingo, Gonzalo J, Shipman, Kelly, Kappe, Stefan H, Kublin, James G, and Murphy, Sean C

Subjects
Plasmodium, lcsh:Arctic medicine. Tropical medicine, lcsh:RC955-962, Reverse Transcriptase Polymerase Chain Reaction, Research, mRNA, Spliced, Plasmodium falciparum, Gametocyte, lcsh:Infectious and parasitic diseases, DNase, parasitic diseases, RNA, Ribosomal, 18S, lcsh:RC109-216, RNA, Messenger, Antisense, Multiplex Polymerase Chain Reaction, RNA, Protozoan
Abstract

Background Plasmodium gametocytes are sexual stages transmitted to female Anopheles mosquitoes. While Plasmodium parasites can be differentiated microscopically on Giemsa-stained blood smears, molecular methods are increasingly used because of their increased sensitivity. Molecular detection of gametocytes requires methods that discriminate between asexual and sexual stage parasites. Commonly tested gametocyte-specific mRNAs are pfs25 and pfs230 detected by reverse transcription polymerase chain reaction (RT-PCR). However, detection of these unspliced mRNA targets requires preceding DNase treatment of nucleic acids to eliminate co-purified genomic DNA. If gametocyte-specific, spliced mRNAs could be identified, DNase treatment could be eliminated and one-step multiplexed molecular methods utilized. Results Expression data was used to identify highly-expressed mRNAs in mature gametocytes that were also low in antisense RNA expression in non-gametocyte stages. After testing numerous candidate mRNAs, the spliced female Pf3D7_0630000 mRNA was selected as a Plasmodium falciparum gametocyte-specific biomarker compatible with Plasmodium 18S rRNA RT-PCR. This mRNA was only detected in samples containing mature gametocytes and was absent in those containing only asexual stage parasites or uninfected human blood. PF3D7_0630000 RT-PCR detected gametocytes across a wide range of parasite densities in both spiked and clinical samples and agreed with pfs25 RT-PCR, the gold standard for RT-PCR-based gametocyte detection. PF3D7_0630000 multiplexed with Plasmodium 18S rRNA RT-PCR was more sensitive than other spliced mRNA targets for one-step RT-PCR gametocyte detection. Conclusions Because the spliced target does not require DNase treatment, the PF3D7_0630000 assay can be multiplexed with Plasmodium 18S rRNA for direct one-step detection of gametocytes from whole human blood. Electronic supplementary material The online version of this article (doi:10.1186/s12936-017-1863-3) contains supplementary material, which is available to authorized users.

Published
2017

66. Operational Performance of a Plasmodium falciparum Ultrasensitive Rapid Diagnostic Test for Detection of Asymptomatic Infections in Eastern Myanmar

Author

Landier, Jordi, primary, Haohankhunnatham, Warat, additional, Das, Smita, additional, Konghahong, Kamonchanok, additional, Christensen, Peter, additional, Raksuansak, Jathee, additional, Phattharakokoedbun, Pase, additional, Kajeechiwa, Ladda, additional, Thwin, May Myo, additional, Jang, Ihn Kyung, additional, Imwong, Mallika, additional, Wiladphaingern, Jacher, additional, Lwin, Khin Maung, additional, Ling, Clare, additional, Proux, Stephane, additional, Domingo, Gonzalo J., additional, Delmas, Gilles, additional, and Nosten, François H., additional

Published
2018
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68. Performance of a High-Sensitivity Rapid Diagnostic Test for Plasmodium falciparum Malaria in Asymptomatic Individuals from Uganda and Myanmar and Naive Human Challenge Infections

Author

Das, Smita, primary, Jang, Ihn Kyung, additional, Barney, Becky, additional, Peck, Roger, additional, Rek, John C., additional, Arinaitwe, Emmanuel, additional, Adrama, Harriet, additional, Murphy, Maxwell, additional, Imwong, Mallika, additional, Ling, Clare L., additional, Proux, Stephane, additional, Haohankhunnatham, Warat, additional, Rist, Melissa, additional, Seilie, Annette M., additional, Hanron, Amelia, additional, Daza, Glenda, additional, Chang, Ming, additional, Nakamura, Tomoka, additional, Kalnoky, Michael, additional, Labarre, Paul, additional, Murphy, Sean C., additional, McCarthy, James S., additional, Nosten, Francois, additional, Greenhouse, Bryan, additional, Allauzen, Sophie, additional, and Domingo, Gonzalo J., additional

Published
2017
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70. Current and cumulative malaria infections in a setting embarking on elimination: Amhara, Ethiopia

Author

Yalew, Woyneshet G., primary, Pal, Sampa, additional, Bansil, Pooja, additional, Dabbs, Rebecca, additional, Tetteh, Kevin, additional, Guinovart, Caterina, additional, Kalnoky, Michael, additional, Serda, Belendia A., additional, Tesfay, Berhane H., additional, Beyene, Belay B., additional, Seneviratne, Catherine, additional, Littrell, Megan, additional, Yokobe, Lindsay, additional, Noland, Gregory S., additional, Domingo, Gonzalo J., additional, Getachew, Asefaw, additional, Drakeley, Chris, additional, and Steketee, Richard W., additional

Published
2017
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71. Recombinant human G6PD for quality control and quality assurance of novel point-of-care diagnostics for G6PD deficiency

Author

Kahn, Maria, primary, LaRue, Nicole, additional, Zhu, Changcheng, additional, Pal, Sampa, additional, Mo, Jack S., additional, Barrett, Lynn K., additional, Hewitt, Steve N., additional, Dumais, Mitchell, additional, Hemmington, Sandra, additional, Walker, Adrian, additional, Joynson, Jeff, additional, Leader, Brandon T., additional, Van Voorhis, Wesley C., additional, and Domingo, Gonzalo J., additional

Published
2017
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72. Challenges for achieving safe and effective radical cure of Plasmodium vivax: a round table discussion of the APMEN Vivax Working Group

Author

Thriemer, Kamala, primary, Ley, Benedikt, additional, Bobogare, Albino, additional, Dysoley, Lek, additional, Alam, Mohammad Shafiul, additional, Pasaribu, Ayodhia P., additional, Sattabongkot, Jetsumon, additional, Jambert, Elodie, additional, Domingo, Gonzalo J., additional, Commons, Robert, additional, Auburn, Sarah, additional, Marfurt, Jutta, additional, Devine, Angela, additional, Aktaruzzaman, Mohammad M., additional, Sohel, Nayeem, additional, Namgay, Rinzin, additional, Drukpa, Tobgyel, additional, Sharma, Surender Nath, additional, Sarawati, Elvieda, additional, Samad, Iriani, additional, Theodora, Minerva, additional, Nambanya, Simone, additional, Ounekham, Sonesay, additional, Mudin, Rose Nanti Binti, additional, Da Thakur, Garib, additional, Makita, Leo Sora, additional, Deray, Raffy, additional, Lee, Sang-Eun, additional, Boaz, Leonard, additional, Danansuriya, Manjula N., additional, Mudiyanselage, Santha D., additional, Chinanonwait, Nipon, additional, Kitchakarn, Suravadee, additional, Nausien, Johnny, additional, Naket, Esau, additional, Duc, Thang Ngo, additional, Do Manh, Ha, additional, Hong, Young S., additional, Cheng, Qin, additional, Richards, Jack S., additional, Kusriastuti, Rita, additional, Satyagraha, Ari, additional, Noviyanti, Rintis, additional, Ding, Xavier C., additional, Khan, Wasif Ali, additional, Swe Phru, Ching, additional, Guoding, Zhu, additional, Qi, Gao, additional, Kaneko, Akira, additional, Miotto, Olivo, additional, Nguitragool, Wang, additional, Roobsoong, Wanlapa, additional, Battle, Katherine, additional, Howes, Rosalind E., additional, Roca-Feltrer, Arantxa, additional, Duparc, Stephan, additional, Bhowmick, Ipsita Pal, additional, Kenangalem, Enny, additional, Bibit, Jo-Anne, additional, Barry, Alyssa, additional, Sintasath, David, additional, Abeyasinghe, Rabindra, additional, Sibley, Carol H., additional, McCarthy, James, additional, von Seidlein, Lorenz, additional, Baird, J. Kevin, additional, and Price, Ric N., additional

Published
2017
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74. Addressing the gender-knowledge gap in glucose-6-phosphate dehydrogenase deficiency: challenges and opportunities.

Author

Domingo, Gonzalo J, Advani, Nicole, Satyagraha, Ari W, Sibley, Carol H, Rowley, Elizabeth, Kalnoky, Michael, Cohen, Jessica, Parker, Michael, and Kelley, Maureen

Subjects
*GLUCOSE-6-phosphate dehydrogenase deficiency, *GLUCOSE-6-phosphate dehydrogenase, *OPERATIONS research, *SCARCITY, *PLASMODIUM vivax
Abstract

Glucose-6-phosphate dehyrdgoenase (G6PD) deficiency is a common X-linked genetic trait, with an associated enzyme phenotype, whereby males are either G6PD deficient or normal, but females exhibit a broader range of G6PD deficiencies, ranging from severe deficiency to normal. Heterozygous females typically have intermediate G6PD activity. G6PD deficiency has implications for the safe treatment for Plasmodium vivax malaria. Individuals with this deficiency are at greater risk of serious adverse events following treatment with the only curative class of anti-malarials, 8-aminoquinolines, such as primaquine. Quantitative diagnostic tests for G6PD deficiency are complex and require sophisticated laboratories. The commonly used qualitative tests, do not discriminate intermediate G6PD activities. This has resulted in poor understanding of the epidemiology of G6PD activity in females and its corresponding treatment ramifications. New simple-to-use quantitative tests, and a momentum to eliminate malaria, create an opportunity to address this knowledge gap. While this will require additional resources for clinical studies, adequate operational research, and appropriate pharmacovigilance, the health benefits from this investment go beyond the immediate intervention for which the G6PD status is first diagnosed. [ABSTRACT FROM AUTHOR]

Published
2019
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75. Analysis of age-dependent trends in Ov16 IgG4 seroprevalence to onchocerciasis

Author

Golden, Allison, primary, Faulx, Dunia, additional, Kalnoky, Michael, additional, Stevens, Eric, additional, Yokobe, Lindsay, additional, Peck, Roger, additional, Karabou, Potochoziou, additional, Banla, Méba, additional, Rao, Ramakrishna, additional, Adade, Kangi, additional, Gantin, Richard G., additional, Komlan, Kossi, additional, Soboslay, Peter T., additional, de los Santos, Tala, additional, and Domingo, Gonzalo J., additional

Published
2016
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77. Assessment of Point-of-Care Diagnostics for G6PD Deficiency in Malaria Endemic Rural Eastern Indonesia

Author

Satyagraha, Ari W., primary, Sadhewa, Arkasha, additional, Elvira, Rosalie, additional, Elyazar, Iqbal, additional, Feriandika, Denny, additional, Antonjaya, Ungke, additional, Oyong, Damian, additional, Subekti, Decy, additional, Rozi, Ismail E., additional, Domingo, Gonzalo J., additional, Harahap, Alida R., additional, and Baird, J. Kevin, additional

Published
2016
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78. A Recombinant Positive Control for Serology Diagnostic Tests Supporting Elimination of Onchocerca volvulus

Author

Golden, Allison, primary, Stevens, Eric J., additional, Yokobe, Lindsay, additional, Faulx, Dunia, additional, Kalnoky, Michael, additional, Peck, Roger, additional, Valdez, Melissa, additional, Steel, Cathy, additional, Karabou, Potochoziou, additional, Banla, Méba, additional, Soboslay, Peter T., additional, Adade, Kangi, additional, Tekle, Afework H., additional, Cama, Vitaliano A., additional, Fischer, Peter U., additional, Nutman, Thomas B., additional, Unnasch, Thomas R., additional, de los Santos, Tala, additional, and Domingo, Gonzalo J., additional

Published
2016
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79. The challenges of introducing routine G6PD testing into radical cure: a workshop report

Author

Ley, Benedikt, Luter, Nick, Espino, Fe E., Devine, Angela, Kalnoky, Michael, Lubell, Yoel, Ley-Thriemer, Kamala, Baird, J. Kevin, Poirot, Eugenie, Conan, Nolwenn, Kheong, Chong Chee, Dysoley, Lek, Khan, Wasif Ali, Dion-Berboso, April G., Bancone, Germana, Hwang, Jimee, Kumar, Ritu, Price, Ric N., von Seidlein, Lorenz, Domingo, Gonzalo J., Ley, Benedikt, Luter, Nick, Espino, Fe E., Devine, Angela, Kalnoky, Michael, Lubell, Yoel, Ley-Thriemer, Kamala, Baird, J. Kevin, Poirot, Eugenie, Conan, Nolwenn, Kheong, Chong Chee, Dysoley, Lek, Khan, Wasif Ali, Dion-Berboso, April G., Bancone, Germana, Hwang, Jimee, Kumar, Ritu, Price, Ric N., von Seidlein, Lorenz, and Domingo, Gonzalo J.

Abstract

The only currently available drug that effectively removes malaria hypnozoites from the human host is primaquine. The use of 8-aminoquinolines is hampered by haemolytic side effects in glucose-6-phosphate dehydrogenase (G6PD) deficient individuals. Recently a number of qualitative and a quantitative rapid diagnostic test (RDT) format have been developed that provide an alternative to the current standard G6PD activity assays. The WHO has recently recommended routine testing of G6PD status prior to primaquine radical cure whenever possible. A workshop was held in the Philippines in early 2015 to discuss key challenges and knowledge gaps that hinder the introduction of routine G6PD testing. Two point-of-care (PoC) test formats for the measurement of G6PD activity are currently available: qualitative tests comparable to malaria RDT as well as biosensors that provide a quantitative reading. Qualitative G6PD PoC tests provide a binomial test result, are easy to use and some products are comparable in price to the widely used fluorescent spot test. Qualitative test results can accurately classify hemizygous males, heterozygous females, but may misclassify females with intermediate G6PD activity. Biosensors provide a more complex quantitative readout and are better suited to identify heterozygous females. While associated with higher costs per sample tested biosensors have the potential for broader use in other scenarios where knowledge of G6PD activity is relevant as well. The introduction of routine G6PD testing is associated with additional costs on top of routine treatment that will vary by setting and will need to be assessed prior to test introduction. Reliable G6PD PoC tests have the potential to play an essential role in future malaria elimination programmes, however require an improved understanding on how to best integrate routine G6PD testing into different health settings.

Published
2015

80. Targeting vivax malaria in the Asia Pacific: The Asia Pacific Malaria Elimination Network Vivax Working Group

Author

Anstey, Nicholas M., Auburn, Sarah, Ley, Benedikt, Mannion, Kylie, Price, Ric N., Ley-Thriemer, Kamala, Baird, J. Kevin, Battle, Katherine E., Bobogare, Albino, Chancellor, Arna, Chasombat, Sanchai, Cheng, Qin, Domingo, Gonzalo J., Drakeley, Christopher J., Drukpa, Tobgyel, Dysoley, Lek, Espino, Fe Esperanza, Gething, Peter W., Ghimire, Prakash, Anstey, Nicholas M., Auburn, Sarah, Ley, Benedikt, Mannion, Kylie, Price, Ric N., Ley-Thriemer, Kamala, Baird, J. Kevin, Battle, Katherine E., Bobogare, Albino, Chancellor, Arna, Chasombat, Sanchai, Cheng, Qin, Domingo, Gonzalo J., Drakeley, Christopher J., Drukpa, Tobgyel, Dysoley, Lek, Espino, Fe Esperanza, Gething, Peter W., and Ghimire, Prakash

Abstract

The Asia Pacific Malaria Elimination Network (APMEN) is a collaboration of 18 country partners committed to eliminating malaria from within their borders. Over the past 5 years, APMEN has helped to build the knowledge, tools and in-country technical expertise required to attain this goal. At its inaugural meeting in Brisbane in 2009, Plasmodium vivax infections were identified across the region as a common threat to this ambitious programme; the APMEN Vivax Working Group was established to tackle specifically this issue. The Working Group developed a four-stage strategy to identify knowledge gaps, build regional consensus on shared priorities, generate evidence and change practice to optimize malaria elimination activities. This case study describes the issues faced and the solutions found in developing this robust strategic partnership between national programmes and research partners within the Working Group. The success of the approach adopted by the group may facilitate similar applications in other regions seeking to deploy evidence-based policy and practice.

Published
2015

83. The challenges of introducing routine G6PD testing into radical cure: a workshop report

Author

Ley, Benedikt, primary, Luter, Nick, additional, Espino, Fe Esperanza, additional, Devine, Angela, additional, Kalnoky, Michael, additional, Lubell, Yoel, additional, Thriemer, Kamala, additional, Baird, J. Kevin, additional, Poirot, Eugenie, additional, Conan, Nolwenn, additional, Kheong, Chong Chee, additional, Dysoley, Lek, additional, Khan, Wasif Ali, additional, Dion-Berboso, April G., additional, Bancone, Germana, additional, Hwang, Jimee, additional, Kumar, Ritu, additional, Price, Ric N., additional, von Seidlein, Lorenz, additional, and Domingo, Gonzalo J., additional

Published
2015
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85. Suitability of Capillary Blood for Quantitative Assessment of G6PD Activity and Performances of G6PD Point-of-Care Tests

Author

Bancone, Germana, primary, Chu, Cindy S., additional, Chowwiwat, Nongnud, additional, Somsakchaicharoen, Raweewan, additional, Wilaisrisak, Pornpimon, additional, Charunwatthana, Prakaykaew, additional, Bansil, Pooja, additional, McGray, Sarah, additional, Domingo, Gonzalo J., additional, and Nosten, François H., additional

Published
2015
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90. G6PD testing in support of treatment and elimination of malaria: recommendations for evaluation of G6PD tests

Author

Domingo, Gonzalo J., Satyagraha, Ari Winasti, Anvikar, Anup, Baird, Kevin, Bancone, Germana, Bansil, Pooja, Carter, Nick, Cheng, Qin, Culpepper, Janice, Eziefula, Chi, Fukuda, Mark, Green, Justin, Hwang, Jimee, Lacerda, Marcus, McGray, Sarah, Menard, Didier, Nosten, Francois, Nuchprayoon, Issarang, Oo, Nwe Nwe, vonSeidlein, Lorenz, et al., Domingo, Gonzalo J., Satyagraha, Ari Winasti, Anvikar, Anup, Baird, Kevin, Bancone, Germana, Bansil, Pooja, Carter, Nick, Cheng, Qin, Culpepper, Janice, Eziefula, Chi, Fukuda, Mark, Green, Justin, Hwang, Jimee, Lacerda, Marcus, McGray, Sarah, Menard, Didier, Nosten, Francois, Nuchprayoon, Issarang, Oo, Nwe Nwe, vonSeidlein, Lorenz, and et al.

Abstract

Malaria elimination will be possible only with serious attempts to address asymptomatic infection and chronic infection by both Plasmodium falciparum and Plasmodium vivax. Currently available drugs that can completely clear a human of P. vivax (known as “radical cure”), and that can reduce transmission of malaria parasites, are those in the 8-aminoquinoline drug family, such as primaquine. Unfortunately, people with glucose-6-phosphate dehydrogenase (G6PD) deficiency risk having severe adverse reactions if exposed to these drugs at certain doses. G6PD deficiency is the most common human enzyme defect, affecting approximately 400 million people worldwide. Scaling up radical cure regimens will require testing for G6PD deficiency, at two levels: 1) the individual level to ensure safe case management, and 2) the population level to understand the risk in the local population to guide Plasmodium vivax treatment policy. Several technical and operational knowledge gaps must be addressed to expand access to G6PD deficiency testing and to ensure that a patient’s G6PD status is known before deciding to administer an 8-aminoquinoline-based drug. In this report from a stakeholder meeting held in Thailand on October 4 and 5, 2012, G6PD testing in support of radical cure is discussed in detail. The focus is on challenges to the development and evaluation of G6PD diagnostic tests, and on challenges related to the operational aspects of implementing G6PD testing in support of radical cure. The report also describes recommendations for evaluation of diagnostic tests for G6PD deficiency in support of radical cure.

Published
2013

91. G6PD testing in support of treatment and elimination of malaria: recommendations for evaluation of G6PD tests

Author

Domingo, Gonzalo J, primary, Satyagraha, Ari Winasti, additional, Anvikar, Anup, additional, Baird, Kevin, additional, Bancone, Germana, additional, Bansil, Pooja, additional, Carter, Nick, additional, Cheng, Qin, additional, Culpepper, Janice, additional, Eziefula, Chi, additional, Fukuda, Mark, additional, Green, Justin, additional, Hwang, Jimee, additional, Lacerda, Marcus, additional, McGray, Sarah, additional, Menard, Didier, additional, Nosten, Francois, additional, Nuchprayoon, Issarang, additional, Oo, Nwe Nwe, additional, Bualombai, Pongwit, additional, Pumpradit, Wadchara, additional, Qian, Kun, additional, Recht, Judith, additional, Roca, Arantxa, additional, Satimai, Wichai, additional, Sovannaroth, Siv, additional, Vestergaard, Lasse S, additional, and Von Seidlein, Lorenz, additional

Published
2013
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94. Extended Result Reading Window in Lateral Flow Tests Detecting Exposure to Onchocerca volvulus: A New Technology to Improve Epidemiological Surveillance Tools

Author

Golden, Allison, primary, Steel, Cathy, additional, Yokobe, Lindsay, additional, Jackson, Emily, additional, Barney, Rebecca, additional, Kubofcik, Joseph, additional, Peck, Roger, additional, Unnasch, Thomas R., additional, Nutman, Thomas B., additional, de los Santos, Tala, additional, and Domingo, Gonzalo J., additional

Published
2013
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97. HIV-1 Gag p17 presented as virus-like particles on the E2 scaffold from Geobacillus stearothermophilus induces sustained humoral and cellular immune responses in the absence of IFNγ production by CD4+ T cells

Author

Caivano, Antonella, primary, Doria-Rose, Nicole A., additional, Buelow, Benjamin, additional, Sartorius, Rossella, additional, Trovato, Maria, additional, D'Apice, Luciana, additional, Domingo, Gonzalo J., additional, Sutton, William F., additional, Haigwood, Nancy L., additional, and De Berardinis, Piergiuseppe, additional

Published
2010
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