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A detailed quasiclassical trajectory study of the O2+N→NO+O reaction is performed based on ab initio potential-energy surfaces of the 2A′ and 4A′ states. The study is aimed at generating a database of thermally averaged and O2 state-specific rate constants needed for accurate simulations of NO kinetics in high-temperature flow processes. The rate constants obtained show good agreement with the available experimental data and with other quasiclassical trajectory calculations. It is found that the reactant internal energy of the O2+N→NO+O reaction is less effective in enhancing the rate than in the N2+O→NO+N reaction. An analysis of the product vibrational energy shows that NO formed by the O2+N→NO+O reaction has a non-Boltzmann distribution. It is also found that the most populated NO vibrational level is determined by the reactant vibrational energy, while the terminal slope of the NO vibrational distribution is a strong function of the reactant translational temperature.

In this paper the extensive quasiclassical trajectory (QCT) study recently concluded [J. Chem. Phys. 104, 2825 (1996)] is used to model the kinetics of the primary NO formation reaction, N2+O→NO+N, in hypersonic nonequilibrium flows. The QCT data are used to obtain expressions for the thermal rate constant, reactant energy removal, and product energy disposal rates of this reaction. The QCT results are coupled with the continuum conservation flow equations, and these equations are used to simulate the Bow-Shock UltraViolet2 (BSUV2) flow at altitudes between 75 to 87.5 km. It is found that the use of the Macheret and Rich [Chem. Phys. 174, 25 (1993)] vibration–dissociation coupling model along with the QCT rates gives improvements in the NO concentration predictions at altitudes between 80 and 85 km. Also, it is found that the vibrational and rotational temperatures of NO are much higher than that of the N2 and O2 in the gas, in accordance with the BSUV2 measurements. The amount of NO produced in the flow fields at 87.5 km and above is found to be strongly dependent on the free-stream density of atomic oxygen.

The Mars Science Laboratory (MSL) Entry Descent and Landing Instrumentation (MEDLI) suite on MSL entry vehicle heatshield has returned pressure, temperature, and thermal protection system (TPS) performance data acquired during entry. This paper presents performance and characterization data of the MEDLI Integrated Sensor Plug (MISP) embedded in Phenolic-Impregnated Carbon Ablator (PICA) heatshield. The sensor is characterized in arc jet facilities at MSL flight relevant conditions. The performance of the Hollow aErothermal Ablation and Temperature (HEAT) sensor in tracking a moving temperature isotherm through the thickness is evaluated. A close agreement between HEAT sensor depth and measured char depth in arc jet samples is also found. The growth of a fence due to Room-Temperature Vulcanizing (RTV) bonding agent around MISP plugs, which has significant impact on aerothermal reconstruction, is also quantified. The data presented will be used for improved reconstruction of the aerothermal environment and TPS response using MISP flight data.

The Mars Science Laboratory heatshield was designed to withstand a fully turbulent heat pulse based on test results and computational analysis on a pre-flight design trajectory. Instrumentation on the flight heatshield measured in-depth temperatures in the thermal protection system. The data indicate that boundary layer transition occurred at 5 of 7 thermocouple locations prior to peak heating. Data oscillations at 3 pressure measurement locations may also indicate transition. This paper presents the heatshield temperature and pressure data, possible explanations for the timing of boundary layer transition, and a qualitative comparison of reconstructed and computational heating on the as-flown trajectory. Boundary layer Reynolds numbers that are typically used to predict transition are compared to observed transition at various heatshield locations. A uniform smooth-wall transition Reynolds number does not explain the timing of boundary layer transition observed during flight. A roughness-based Reynolds number supports the possibility of transition due to discrete or distributed roughness elements on the heatshield. However, the distributed roughness height would have needed to be larger than the pre-flight assumption. The instrumentation confirmed the predicted location of maximum turbulent heat flux near the leeside shoulder. The reconstructed heat flux at that location is bounded by smooth-wall turbulent calculations on the reconstructed trajectory, indicating that augmentation due to surface roughness probably did not occur. Turbulent heating on the downstream side of the heatshield nose exceeded smooth-wall computations, indicating that roughness may have augmented heating. The stagnation region also experienced heating that exceeded computational levels, but shock layer radiation does not fully explain the differences.

The Mars Science Laboratory entry vehicle successfully landed the Curiosity rover on the Martian surface on August 5, 2012. A phenolic impregnated carbon ablator heatshield was used to protect the spacecraft against the severe aeroheating environments of atmospheric entry. This heatshield was instrumented with a comprehensive set of pressure and temperature sensors. The objective of this paper is to perform an inverse estimation of the entry vehicle’s surface heating and heatshield material properties. The surface heating is estimated using the flight temperature data from the shallowest thermocouple. The sensitivity of the estimated surface heating profile to estimation tuning parameters, measurement errors, recession uncertainty and material property uncertainty is investigated. A Monte Carlo analysis is conducted to quantify the uncertainty bounds associated with the nominal estimated surface heating. Additionally, a thermocouple driver approach is employed to estimate heatshield material properties using the flight data from the deeper thermocouples while applying the shallowest thermocouple temperature as the surface boundary condition.

The Mars Science Laboratory successfully landed on the Martian surface on August 5th, 2012. The rover was protected from the extreme heating environments of atmospheric entry by an ablative heatshield. This Phenolic Impregnated Carbon Ablator heatshield was instrumented with a suite of embedded thermocouples, isotherm sensors, and pressure transducers. The sensors monitored the in-depth ablator response, as well as the surface pressure at discrete locations throughout the hypersonic deceleration. This paper presents a comparison of the flight data with post-entry estimates. An assessment of the aerothermal environments, as well as the in-depth response of the heatshield material is made, and conclusions regarding the overall performance of the ablator at the suite locations are presented.

We simultaneously measured intracellular pH (pHi) and isometric tension in canine basilar arteries. pHi was calculated from the ratio of fluorescence intensities at 540 nm of exciting wavelengths of 500 and 440 nm in the presence of 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF). pHi was 7.33 +/- 0.02 in Krebs-Henseleit solution (pH of 7.4 at 37 degrees C). Application of the anion exchange blocker 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid (SITS) decreased the resting pHi by 0.25 pH units. Increasing extracellular pH (pHo) [by decreasing CO2 tension (PCO2)] from 7.4 to 7.8 increased pHi by 0.38 pH units and increased tension by 2.28 +/- 0.21 mN. Decreasing pHo from 7.8 to 7.4 (by increasing PCO2) restored the pHi and muscle tension to their baseline levels. SITS inhibited the increase in pHi and isometric tension in response to the increase in pHo in an endothelium-independent fashion. The Na+/H+ exchange blockers, amiloride or 5-(N-methyl-N-guanidinocarbonylmethyl)-amiloride, did not affect the pHo or tension changes. The results suggest that in the range of pH tested, anion exchange is more important than Na+/H+ exchange in the regulation of pHi and mechanical tone in the basilar artery.

Theoretical determinations of the thermal rate constants and product energy distributions of the N2+O→NO+N reaction, which plays a crucial role in hydrocarbon air combustion and high temperature air chemistry, are carried out using a quasiclassical trajectory method. An analytical fit of the lowest 3A′ potential energy surface of this reaction based on the CCI ab initio data is obtained. The trajectory study is done on this surface and an analytical 3A″ surface proposed by Gilibert et al. [J. Chem. Phys. 97, 5542 (1992)]. The thermal rate constants computed from 3000 to 20 000 K are in good agreement with the available experimental data. In addition, the dependence of the rate constant on the N2 internal state is studied. It is found that a low vibrational excitation can reduce the rate constant of this reaction by a factor of 3. Also, we investigate the effect of the N2 vibrational state on the product NO vibrational distribution, and it is found that at low N2 vibrational states, the NO vibrational dist...

The Mars Science Laboratory (MSL) Entry Descent and Landing Instrumentation (MEDLI) suite on MSL entry vehicle heatshield has successfully returned pressure, temperature, and thermal protection system (TPS) ablation data acquired during entry. This paper provides an initial assessment of MEDLI thermal instrumentation data that is comprised of in-depth temperatures in the TPS made of Phenolic-Impregnated Carbon Ablator (PICA). Temperatures are measured in-depth at seven different locations on the surface. The thermal sensor plugs are also characterized in arc jet facilities to quantify measurement uncertainties and biases. The assessment of flight data provides key insights into boundary layer transition to turbulence, surface recession, turbulent heating augmentation, stagnation point and apex laminar heating, and in-depth thermal response. A preliminary comparison with model results highlights inadequacies in our predictive capability. The peak temperature measured by near surface thermocouples was found to be 1049 C in the vicinity of apex region. Initial estimate of peak surface temperature with nominal model settings is about 1575 C. The peak heat flux was found to be on the leeside of the vehicle as predicted, but its value is sensitive to the recession model.

The Mars Science Laboratory (MSL) entry vehicle successfully landed on the Martian surface on August 5, 2012. A phenolic impregnated carbon ablator heatshield was used to protect the spacecraft against the severe aeroheating environments of atmospheric entry. This heatshield was instrumented with a comprehensive set of pressure and temperature sensors. The objective of this paper is to present the thermal ight data returned and provide a preliminary postight analysis of MSL’s aerothermal environment and heatshield thermal response. The ight temperature data are compared with the thermal response predictions by the same analytical models used in heatshield design. In addition to this direct comparison, a preliminary inverse analysis is performed where the time-dependent surface heating is estimated from ight-measured subsurface temperature data.

This paper presents an overview of the analysis and measurements of equilibrium radiation obtained in the NASA Ames Research Center's Electric Arc Shock Tube (EAST) facility as a part of recent testing aimed at reaching shock velocities up to 15.5 km/s. The goal of these experiments was to measure the level of radiation encountered during high speed Earth entry conditions, such as would be relevant for an asteroid, inter-planetary or lunar return mission. These experiments provide the first spectrally and spatially resolved data for high speed Earth entry and cover conditions ranging from 9.5 to 15.5 km/s at 13.3 and 26.6 Pa (0.1 and 0.2 Torr). The present analysis endeavors to provide a validation of shock tube radiation measurements and simulations at high speed conditions. A comprehensive comparison between the spectrally resolved absolute equilibrium radiance measured in EAST and the predictive tools, NEQAIR and HARA, is presented. In order to provide a more accurate representation of the agreement between the experimental and simulation results, the integrated value of radiance has been compared across four spectral regions (VUV, UV/Vis, Vis/NIR and IR) as a function of velocity. Results have generally shown excellent agreement between the two codes and EAST data for the Vis through IR spectral regions, however, discrepancies have been identified in the VUV and parts of the UV spectral regions. As a result of the analysis presented in this paper, an updated parametric uncertainty for high speed radiation in air has been evaluated to be [9.0%, -6.3%]. Furthermore, due to the nature of the radiating environment at these high shock speeds, initial calculations aimed at modeling phenomena that become more significant with increasing shock speed have been performed. These phenomena include analyzing the radiating species emitting ahead of the shock and the increased significance of radiative cooling mechanisms.

Within shock waves, the translational motion of the gas is more energetic in the direction perpendicular to the shock than in the direction parallel to the shock. To represent this translational nonequilibrium, new continuum conservation equations are developed. These equations are derived by solving the Boltzmann equation with a first‐order Chapman–Enskog expansion of an anisotropic velocity distribution function. This results in a gas model with anisotropic pressure, temperature, and speed of sound. The governing equations are solved numerically for one‐dimensional steady shock waves in a Maxwellian gas. The numerical results are compared to those obtained using the direct simulation Monte Carlo method. The new continuum model captures many of the features of shock waves. In particular, this paper finds that translational nonequilibrium is present at all Mach numbers. For Mach numbers greater than 1.5, the perpendicular temperature overshoots the post‐shock temperature. At the point where this temperature reaches a maximum, the model predicts that for any shock wave, the square of the perpendicular‐direction Mach number is one‐third; this is substantiated by the DSMC results.

The present paper provides the background of a focused effort to assess uncertainties in predictions of heat flux and pressure in hypersonic flight (airbreathing or atmospheric entry) using state-of-the-art aerothermodynamics codes. The assessment is performed for four mission relevant problems: (1) shock turbulent boundary layer interaction on a compression corner, (2) shock turbulent boundary layer interaction due a impinging shock, (3) high-mass Mars entry and aerocapture, and (4) high speed return to Earth. A validation based uncertainty assessment approach with reliance on subject matter expertise is used. A code verification exercise with code-to-code comparisons and comparisons against well established correlations is also included in this effort. A thorough review of the literature in search of validation experiments is performed, which identified a scarcity of ground based validation experiments at hypersonic conditions. In particular, a shortage of useable experimental data at flight like enthalpies and Reynolds numbers is found. The uncertainty was quantified using metrics that measured discrepancy between model predictions and experimental data. The discrepancy data is statistically analyzed and investigated for physics based trends in order to define a meaningful quantified uncertainty. The detailed uncertainty assessment of each mission relevant problem is found in the four companion papers.

This paper presents an overview of the analysis and measurements of equilibrium radiation obtained in the NASA Ames Research Center’s EAST facility as a part of the Multi-Purpose Crew Vehicle (MPCV) Aerosciences Project (CAP). These experiments were aimed at measuring the level of radiation encountered during conditions relevant to Orion lunar return into Earth’s atmosphere. Therefore, the targeted conditions ranged from 8 to 12 km/s and 13.3 to 133.3 Pa (0.1 to 1.0 Torr). A comprehensive comparison between the spectrally resolved absolute radiance measured on EAST and the predictive tools, NEQAIR and HARA, is presented. In order to provide a more accurate representation of the uncertainty, the current predictive capability has been calculated as a function of velocity. The results of the analysis have shown that both models under-predict the EAST results at lower speeds, with the agreement improving with increasing shock speed. Potential reasons for the discrepancies are provided in the paper. Furthermore, power law coecients characterizing the relationship of intensity versus velocity as measured on EAST are presented for ease of conducting experimental and computational comparisons in the future.

We examined whether depressed left ventricular (LV) contractility during Escherichia coli sepsis in dogs was due to a decrease in the fractional release of calcium from the sarcoplasmic reticulum (SR) or a reduction in calcium content in this organelle. To indirectly assess SR calcium availability in a right ventricular (RV) trabecular muscle preparation, we utilized functional indexes of cellular myocardial calcium metabolism, which included rapid-cooling contracture (RCC), an indicator of SR calcium content, and postrest contraction (PRC), an index of calcium availability from the release compartment of the SR. Measurements were made during steady-state stimulation at 0.5 and 1.5 Hz, during which time rest intervals of 30-240 s were periodically imposed. SR calcium availability was measured in RV trabeculae of dogs subjected to 4 h of E. coli sepsis and was compared with calcium availability measured in nonseptic dogs. We further characterized a filterable cardiodepressant substance (FCS), which has been previously shown to be associated with LV depression in this model, to determine whether it produced changes in calcium metabolism similar to those found in sepsis. The results showed that calcium availability from the SR of septic dogs was not impaired. Furthermore, FCS was found in the 10,000- to 30,000-mol wt fraction of plasma and produced changes in PRC in canine trabeculae that were similar to those produced during sepsis. We conclude that, as assessed by PRC and RCC, SR calcium content and release are not impaired in sepsis.

Comparisons of experimental characterization and model predictions of entry radiation relevant to Mars and Venus exploration are presented. Characterization is performed in the recently upgraded Ames Electric Arc Shock Tube (EAST) facility. Tests are performed in Mars (96% CO2, 4% N2) and Venus (96.5% CO2, 3.5% N2) simulant gases at downstream pressures and incident velocities spanning from 0.1-2.0 Torr and 3-12 km/s. Velocity and pressure conditions were chosen based on expected flight conditions (direct entry or aerocapture) in Mars and Venus atmospheres. The absolute radiance data are spatially and spectrally resolved and span the vacuum ultraviolet (VUV) through mid-IR (120-1650 nm, 35 μm). Resolved spectra of the CO 4 positive band in the VUV are reported for the first time. Measurements of CO2 molecular vibrational radiation is also attempted at low velocity conditions. Radiation modeled under equilibrium assumption with NEQAIR code compares favorably to measured radiation under some, but not all, conditions.

This paper presents measurements of equilibrium radiation obtained in the NASA Ames Research Center's EAST facility and the University of Queensland's X2 facility. These experiments were aimed at measuring the level of radiation encountered during conditions relevant to Orion lunar return into Earth's atmosphere. The facilities have targeted the same nominal test conditions of 10 km/s and 26.6 Pa (0.2 Torr). In addition, variations on the nominal shock speed have also been the focus of recent testing in the EAST facility. A comprehensive comparison between the EAST data and NEQAIR is presented in this paper with preliminary X2 comparisons where appropriate. Since the two facilities have different dimensions, and the tests have different shock speeds, NEQAIR simulations are used as a point of reference for the EAST and X2 comparison. Results obtained by independently reducing the data from both facilities are compared. The present analysis endeavors to provide a better understanding of the uncertainty in the measurements, as well as provide an initial comparison between EAST and X2. Furthermore, the present analysis explores various radiative mechanisms to determine if they are due to physical processes relevant to flight, or are just facility dependent phenomena. These phenomena include effects such as the magnitude of the background continuum.

The aim of this work was to determine the relationship between peak twitch amplitude and sarcoplasmic reticulum (SR) Ca2+ content during changes of stimulation frequency in isolated canine ventricle, and to estimate the extent to which these changes were dependent upon sarcolemmal Na(+)-Ca2+ exchange. In physiological [Na+]o, increased stimulation frequency in the 0.2-2-Hz range resulted in a positive inotropic effect characterized by an increase in peak twitch amplitude and a decrease in the duration of contraction, measured as changes in isometric force development or unloaded cell shortening in intact muscle and isolated single cells, respectively. Action potentials recorded from single cells indicated that the inotropic effect was associated with a progressive decrease of action potential duration and a marked reduction in average time spent by the cell near the resting potential during the stimulus train. The frequency-dependent increase of peak twitch force was correlated with an increase of Ca2+ uptake into and release from the SR. This was estimated indirectly using the phasic contractile response to rapid (less than 1 s) lowering of perfusate temperature from 37 degrees C to 0-2 degrees C and changes of twitch amplitude resulting from perturbations in the pattern of electrical stimulation. Lowering [Na+]o from 140 to 70 mM resulted in an increase of contractile strength, which was accompanied by a similar increase of apparent SR Ca2+ content, both of which could be abolished by exposure to ryanodine (1 x 10(-8) M), caffeine (3 x 10(-3) M), or nifedipine (2 x 10(-6) M). Increased stimulation frequency in 70 mM [Na+]o resulted in a negative contractile staircase, characterized by a graded decrease of peak isometric force development or unloaded cell shortening. SR Ca2+ content estimated under identical conditions remained unaltered. Rate constants derived from mechanical restitution studies implied that the depressant effect of increased stimulation frequency in 70 mM [Na+]o was not a consequence of a decreased rate of refilling of a releasable pool of Ca2+ within the cell. These results demonstrate that frequency-dependent changes of contractile strength and intracellular Ca2+ loading in 140 mM [Na+]o require the presence of a functional sarcolemmal Na(+)-Ca2+ exchange process. The possibility that the negative staircase in 70 mM [Na+]o is related to inhibition of Ca(2+)-induced release of Ca2+ from the SR by various cellular mechanisms is discussed.

This paper presents ground-to-flight traceability analysis of arcjet testing of thermal protection system materials developed for NASA’s Crew Exploration Vehicle. It is shown that ground facilities are limited in simulating many key aerothermal environment parameters, such as enthalpy, shear, radiative heating, boundary layer thickness, etc. The limits of the facilities are more apparent when multi-parameter combinations of aerothermal parameters are desired. The importance of using design of experiments techniques is also highlighted in order to address ground-to-flight traceability concerns by separating the effects of each environment parameter and their interactions. A discussion on observed recession rates in arcjets and mathematical model validation, especially, in a non-stagnation environment is also presented. (CEV-TPS-ADP) recently concluded a 3-year technology maturation program to develop, test, and downselect heat shield materials for Orion lunar entry. Two lunar return capable TPS concepts were developed by the TPS ADP until its conclusion in 2009. The first material is Phenolic Impregnated Carbon Ablator (PICA) 2 with flight heritage in Stardust Sample Return Capsule. It was also adopted by Mars Science Laboratory mission, 3 leveraging heavily from the TPS ADP development. The second material developed by the TPS ADP is Avcoat with Apollo fight heritage. Ablators such as PICA and Avcoat are among the few viable lightweight materials capable of withstanding severe aerothermal environment encountered during lunar return entry. In 2009, after significant testing and analysis, a final down-selection of Avcoat as the recommended system for Orion TPS was made based on a variety of criteria related to performance, reliability, and maintainability.

An update of NEQAIR is presented and used to compare with experiment in the presence of thermal equilibrium. The updates included boundbound transition probabilities of C, N, and O, Stark broadening expressions, cross sections for bound-free transitions and additional transitions bands for N2. Moreover, the role of carbon contamination is examined. Some improvements are indicated but gaps remain between experiment and computation in the ultraviolet region of the spectrum. The discrepancies are not a result of ignoring the carbon contaminant.

Summary Myocardial pump failure during ischemia and hypoxia is often a reversible dysfunction. Contractile changes can occur in several different ways: during acute ischute ischemia, during chronic ischemia as an adaptive process, and during reperfusion following ischemia. Both systolic and diastolic contractile properties are impaired. Although a number of processes, e.g., the failure of the action potential generation mechanisms and reduced energy supply, have been suspected as causes of systolic impairment, the most likely factors appear to be H+ and inorganic phosphate concentrations. The increase in diastolic stiffness is most likely due to an increase in intracellular Ca2+ and, in severe instances, to rigor linkages between actin and myosin filaments. The extent of diastolic dysfunction depends on the nature of ischemia, being more marked in demand ischemia as compared with supply ischemia. Useful criteria to delineate the transition from reversible to irreversible ischemic damage are lacking. Nevertheless, assessment of the reversibility of ischemic contractile changes by nitroglycerin, inotropes, and postextrasystolic potentiation before surgical correction of the flow obstruction may have value in predicting outcome. Several adaptive biochemical changes are known to occur after stress. Hyperthermia produces a family of heat stress proteins that have been found to offer protection against ischemic cardiac damage. Future studies need to be performed to understand the mechanism of the regulation of production of these substances and how they afford protection to the ischemic myocardium. Furthermore, improvements in methods for imaging cardiac metabolic and perfusion status and the development of newer pharmacologic tools to alter intracellular pH regulation are expected to improve our understanding of the mechanism of ischemic cardiac damage and offer better possibilities of therapeutic management.

This retrospective study was undertaken to examine the management and outcome of children who developed isolated masseter muscle spasm (MMS) after the administration of intravenous succinylcholine during anesthetic induction. The inhalation anesthetics used for induction were continued in all of these cases. The medical records of 68 patients (male/female ratio, 1.7:1), identified from approximately 42,000 anesthetics given during the period 1980-1989, were reviewed. Fifty-seven children (2.3-12 yr old) were diagnosed as having isolated MMS, i.e., MMS without spasm of other muscles; 11 experienced generalized rigidity in combination with MMS. Anesthetic and postoperative management of these two groups differed. The overall incidence of MMS was 0.3% of inhalation anesthetics during which succinylcholine was given. Intraoperative arrhythmias occurred in 33% of the patients who developed isolated MMS and more frequently in older children. Most children experienced some degree of hypercarbia and/or metabolic acidosis, but the significance of these abnormalities in the spontaneously ventilating, fasting child is unknown. Serum creatine kinase levels when measured 18-24 h postoperatively were elevated in all but one child (n = 45). There was no long-term morbidity and no mortality. We conclude that failure of the masseter muscles to relax after succinylcholine is not uncommon in children. Based on our experience, and accepting that MMS may be part of the clinical spectrum of malignant hyperthermia, we believe that anesthesia can be continued safely in cases of isolated MMS when careful monitoring accompanies diagnostic evaluation. This differs from the current practice of discontinuing the anesthetic or switching to a nontriggering anesthetic technique.

We examined whether the decrease in cardiac contractility in streptozotocin-induced diabetes in the rat is accompanied by reduced or excessive loading of the sarcoplasmic reticulum (SR) with Ca2+. Pooled SR Ca2+ content and fractional release on stimulation were estimated with rapid cooling contracture (RCC) and twitch height measurements, respectively. Interval-force relation was studied to assess the ability of diabetic tissue to alter the relative contribution of SR Ca2+ for contraction. Two months after injection with streptozotocin, peak isometric contraction and steady-state RCC decreased in parallel to approximately 50% of control values. The time to peak force development and complete relaxation was prolonged to 156 and 161% in diabetes in the presence of 1.25 and 2.5 mM extracellular Ca2+ concentration [Ca2+]o, respectively. A stepwise increase in the rate of stimulation from 0.2 to 0.5 and 1.0 Hz resulted in a negative force staircase, the slope of which was identical in control and diabetic animals in each [Ca2+]o tested. Postrest contractions and RCC, after variable test intervals, were significantly depressed after 0.2 and 0.5 Hz stimulation in diabetic muscles at 1.25 mM [Ca2+]o. This defect of SR Ca2+ availability was reversed by increasing the stimulation frequency to 1.0 Hz or by elevating [Ca2+]o to 2.5 mM. The results suggest that the marked reduction of developed tension in diabetic tissues was a consequence of depleted SR Ca2+ stores, rather than a result of chronic SR Ca2+ overloading. The maintained integrity of the interval-force relation in the presence of diabetes implies that the cellular mechanisms responsible for frequency- and time-dependent alterations in SR Ca2+ availability are not disturbed at this stage of disease.

Spectrally resolved emission measurements have been obtained recently in the NASA Ames Research Center (ARC) Electric Arc Shock Tube (EAST) Facility at test conditions relevant to lunar return missions. A subset of these measurements have been analyzed to find the Local Thermodynamic Equilibrium (LTE) temperature using different methods to determine whether or not the spectra were obtained at equilibrium conditions. This LTE verification study has been undertaken to support the development of a benchmark data set for the verification of radiative heat transfer calculations for lunar return trajectories. Quanti tative assessment of LTE for the test conditions is the first step in the evaluation of spectral models of Earth atmosphere shock-induced emission, which is a prerequisite to the evaluation of non-equilibr ium spectral modeling.

This paper analyzes the shock layer radiative heating environment for a large entry vehicle on a lunar return trajectory. Modeling results show that much of the shock layer plasma is in local thermodynamic equilibrium (LTE) and is not optically thin. The ionization level is generally high (15%) and the air is almost fully dissociated. A significant amount of vacuum ultraviolet (VUV) radiation is produced due to bound-bound and bound-free transitions of N and O atoms. The sensitivity of total radiation to Stark broadening, which dominates over other line broadening mechanisms, is quantified. The latter part of this paper reports the status of ongoing validation of the current radiation models with measurements in the Electric-Arc Shock Tube (EAST) facility at NASA Ames Research Center. Model predictions are compared with the calibrated radiation spectra measured in the equilibrium portion of the shock layer at 0.3 Torr. The reasons for discrepancy between model and measurements are also discussed with possible hypotheses presented for further investigation.

An investigation of the aerothermodynamic environment of the Huygens entry probe has been conducted. A Monte Carlo simulation of the trajectory of the probe during entry into Titan's atmosphere was performed to identify a worst-case heating rate trajectory. Flowfield and radiation transport computations were performed at points along this trajectory to obtain convective and radiative heat-transfer distributions on the probe's heat shield. This investigation identified important physical and numerical factors, including atmospheric CH4 concentration, transition to turbulence, numerical diffusion modeling, and radiation modeling, which strongly influenced the aerothermodynamic environment.

Assessment of nonequilibrium thermochemical models for shock layer radiation in N2/CH4 mixtures is presented via comparisons with spectrally and temporally resolved intensity measurements from a set of shock tube experiments. The experiments were carried out at the Electric Arc Shock Tube (EAST) facility at NASA Ames Research Center in a rarified environment [13.3-133.3 Pa (0.1 and 1 Torr)] representative of the peak heating conditions of a Titan aerocapture trajectory (5-9 km/s). The baseline model that assumes a Boltzmann population of the CN excited states consistently overpredicts the shock layer radiation intensity. A non-local collisional radiative model that solves a simplified master equation and includes radiative transport and non-local absorption in the shock tube is presented. The proposed model improves the prediction of the nonequilibrium radiation overshoot peak, but still underpredicts the intensity decay rate in the low pressure case. Further analysis suggests possible reasons for the remaining disagreement, the most likely being a slow CN consumption in the current chemical kinetics model in the intensity fall-off region.

Inflammatory mediators have been implicated as a cause of reversible myocardial depression in septic shock. We previously reported that the release of lysozyme-c (Lmz-S) from leukocytes from the spleen or other organs contributes to myocardial dysfunction in Escherichia coli septic shock in dogs by binding to a cardiac membrane glycoprotein [J. Mol. Cell. Cardiol. 35 (2003) 265]. However, the mechanism by which Lzm-S causes this depression has not been elucidated. In the present study, we tested the hypothesis that the binding of Lzm-S to a membrane glycoprotein causes myocardial depression by the formation of nitric oxide (NO). NO generation then activates soluble guanylyl cyclase and increases cyclic guanosine monophosphate (cGMP), which in turn triggers contractile impairment via activation of cGMP-dependent protein kinase (PKG). We examined these possibilities in a right ventricular trabecular preparation in which isometric contraction was used to measure cardiac contractility. We found that Lzm-S's depressant effect could be prevented by the non-specific NO synthase (NOS) inhibitor NG-monomethyl- l -arginine ( l -NMMA). A guanylyl cyclase inhibitor (ODQ) and a PKG inhibitor (Rp-8-Br-cGMP) also attenuated Lzm-S's depressant effect as did chemical denudation of the endocardial endothelium (EE) with Triton X-100 (0.5%). In EE tissue, we further showed that Lzm-S caused NO release with use of 4,5 diaminofluorescein, a fluorescent dye that binds to NO. The present study shows that the binding of Lzm-S to EE generates NO, and that NO then activates the myocardial guanosine 3′,5′ monophosphate pathway leading to cardiac depression in sepsis.

A Monte Carlo uncertainty and sensitivity analysis technique is presented to i) identify the major sources of uncertainty in the thermochemical models used for aerothermal analysis, and ii) track the propagation of these uncertainties through the system into the predicted quantities of interest, such as the vehicle heating, shock layer properties, etc. The technique is applied to the aerothermal analysis of Titan aerocapture, where CN shock layer radiation is the dominant source of vehicle heating. Several hundred model input parameters, including reaction rate constants, vibration-chemistry coupling parameters, vibrational relaxation times, and transport properties, are independently sampled over their range of uncertainties, and the vehicle heating is determined probabilistically. A massively parallel, axisymmetric CFD (Data-Parallel Line Relaxation) code was used to make the several thousand runs needed to statistically describe the variability in the heating predictions. It is found that major contributions to the uncertainty in the predicted heating originates from the uncertainties in the rates of N2 dissociation by H atom impact, and some atomic exchange reactions: N2+H→NH+N and N2+C→CN+N.

In sepsis, reversible myocardial depression has been ascribed to the release of mediators of inflammation. We previously found that lysozyme released from leukocytes from the spleen and other organs mediated myocardial depression in an Escherichia coli model of septic shock in dogs. We hypothesize that lysozyme binds to or cleaves a cardiac surface membrane N-glycoprotein to cause depression. The objectives of the present study were: 1) to determine whether the binding of lysozyme is reversible; 2) to assess the N-glycan structure to which lysozyme binds; 3) to examine whether nonenzymatic proteins, termed lectins, with a binding specificity similar to that of lysozyme could also cause depression; and 4) to assess whether the membrane to which lysozyme binds is affected by the enzymes protease type XIV and collagenase A, that are used to prepare single cell myocyte experiments.We measured isometric contraction in a right ventricular trabecular preparation.We found that lysozyme binds in a reversible manner to the Man beta(1-4) GlcNAc beta(1-4)GlcNAc moiety in the tri-mannosyl core structure of high mannose/hybrid and tri-antennary carbohydrate classes where GlcNAc is N-acetylglucosamine and Man is mannose. Lectins with a specificity similar to that of lysozyme also caused depression, and lysozyme's depressant activity was eliminated by protease type XIV and collagenase A.These results indicate that lysozyme reversibly binds to a membrane glycoprotein to cause myocardial depression in sepsis. We further localize its binding site to a variant of the chitotriose structure in the tri-mannosyl core of the membrane glycoprotein.

Objective Reversible myocardial depression in sepsis has been ascribed to the release of inflammatory mediators. We recently found that lysozyme c (Lzm-S), consistent with that originating from the spleen, was a mediator of myocardial depression in an Escherichia coli model of septic shock in dogs. We further showed in a right ventricular trabecular (RVT) preparation that Lzm-S’s depressant activity could be blocked by N,N′,N’ triacetylglucosamine (TAC), a competitive inhibitor of Lzm-S. We hypothesized that Lzm-S binds to or cleaves a cardiac membrane glycoprotein, thereby interfering with myocardial contraction in sepsis. In the present study, we examined whether TAC could prevent myocardial depression in an in vivo preparation and whether other related N-acetylglucosamine (NAG) structures could also inhibit Lzm-S’s effect in RVT. Design Randomized experimental study. Setting University laboratory. Subjects Anesthetized, mechanically ventilated dogs. Interventions We produced sepsis by infusion of E. coli over an approximately 6-hr period. Measurements and Main Results We examined the effect of TAC on stroke work, our primary index of myocardial function, when treatment was administered before sepsis (pretreatment) and after 1.5 hrs (early treatment study) and 3.5 hrs of sepsis (late treatment study; LTS). In the pretreatment study and early treatment study, myocardial depression would have not yet occurred but would have already been present in the late treatment study. In RVT, we assessed the effect of other NAG oligosaccharides and variants to the NAG structure on Lzm-S’s depressant activity. In pretreatment and the early treatment study, TAC prevented the reduction in stroke work observed in nontreated septic groups but did not reverse the reduction found in the late treatment study. In RVT, of the compounds tested, only N,N′-diacetylglucosamine showed an inhibitory effect. Conclusions We found that TAC, a competitive inhibitor of Lzm-S, prevented myocardial depression in experimental sepsis. Only specific NAG structures are inhibitory to Lzm-S’s depressant activity. TAC may be useful in attenuating cardiovascular collapse in sepsis.

Objective: Lactic acidosis is an important complication of septic shock. Alkali treatment such as sodium bicarbonate is often used to treat the low pH level that develops in sepsis. The effect of this treatment on lactate (Lac) clearance is not clear. In the present study, the objective was to examine whether blood pH level alters Lac metabolism in sepsis. Measurements were determined in a canine model of Escherichia coli sepsis after bolus infusion (5 mmol/kg) of either lactic acid (LA) or sodium lactate (NaL). In one preparation, Lac uptake by the splanchnic organs (SP), liver, lung, kidneys (Kid), and soft tissues of the lower extremity (SOL) was primarily determined, whereas in another preparation, Lac uptake by the head and neck region and lung was obtained. Methods: The dogs were studied while anesthetized and ventilated. After 4 hours of sepsis, either LA or NaL was given through a catheter positioned in the abdominal aorta in respective sepsis (SepLA, SepNaL) and nonsepsis groups (ConLA, ConNaL) (n ≈ 6 in each preparation). Catheters and flow probes were used to measure organ Lac uptake. Measurements were obtained at end infusion and at 15-minute intervals after infusion until 75 minutes after infusion. Results: Arterial clearance of Lac in the sepsis groups was slower as compared with the nonsepsis groups. In the liver, sepsis inhibited the uptake of LA as compared with the nonseptic group. In SP, both sepsis and pH affected Lac uptake in which an increase in uptake was found only after NaL infusion in the nonseptic group. In the head and neck region, Lac uptake was pH-level dependent and was found after LA infusion in the sepsis and nonsepsis groups. In the lung, Lac was produced after either LA or NaL infusion in all groups. Neither Kid nor SOL contributed to Lac uptake in any of the groups. Conclusion: Lactate clearance was reduced in sepsis. Both effects of pH level and sepsis modulated the organ uptake of Lac in septic shock. Only a small amount of the total Lac infused could be accounted for by the organs measured in the present study. This suggests that additional organs may account for lactate removal in sepsis. Copyright 2002, Elsevier Science (USA). All rights reserved.

Objective: In the heart, histamine H 3 receptors may function as inhibitory presynaptic receptors that decrease adrenergic neural norepinephrine release in conditions of enhanced sympathetic tone. In a previous study, we found that H 3 receptor blockade improved cardiac contractility and systemic hemodynamics in experimental bacteremia in dogs. Because histamine H 3 receptors have been found in the splanchnic circulation in other animal models, it was not clear the extent to which H3 receptor blockade may have altered splanchnic hemodynamics, and variables of venous return, that in turn contributed to the overall improvement in systemic hemodynamics observed in the previous experiment. In the present study, we examined splanchnic hemodynamics in the presence of H3 receptor blockade in a canine model of Escherichia coli bacteremia. Design: Bacteremia was produced by intravenous infusion of live E. coli administered throughout the experiment. Variables of venous return included mean systemic pressure, resistance to venous return, and mean right atrial pressure. Splanchnic measurements included hepatic and portal pressures and flows. Measurements were obtained before and after H3 receptor blockade with thioperamide maleate. The animals were studied while ventilated and anesthetized. Results: H3 receptor blockade caused a decrease in mean right atrial pressure from 5.9 mm Hg pretreatment to 3.5 mm Hg posttreatment (p

We report some of the unique pharmacological properties of a semipurified endogenous inotropic factor (EIF) present in the extract of the porcine left ventricle. EIF produced the following effects: (a) increase in isometric contractile force developed by electrically driven canine right ventricular trabecula, reaching a maximum with 60-100 microliters/ml concentration; (b) inhibition of Na-pump activity in canine portal vein; (c) no digitalis-like cardiac toxicity, e.g., increased diastolic tension or spontaneous diastolic mechanical oscillatory activity, despite inhibition of the sodium pump; (d) a small increase in sarcoplasmic reticular Ca release from the heart but a large increase in transsarcolemmal Ca influx as seen in biphasic contractions, an action similar to that produced by digitalis-like substances; and (e) prolongation of the action potential duration and refractory period of the canine isolated trabeculae. This latter action may confer a unique antiarrhythmic property to EIF.

In septic shock, the extent to which lactic acidosis (LA) is a consequence of splanchnic lactate overproduction (SLP) or impaired hepatic lactate extraction (HLE) is not clear. We examined SLP and HLE in E. coli sepsis in dogs. We further determined the effects of vasopressor treatments, which included phenylephrine, dopamine, norepinephrine, and a combination of dobutamine and norepinephrine treatment, on SLP and HLE in respective groups. The animals were studied while anesthetized and ventilated. During sepsis, SLP increased as compared with presepsis (-0.017 versus 0.07 mmol/min, p < 0.05), but this increase could not be explained by reduced splanchnic oxygen delivery (SOD). During sepsis, HLE increased as compared with baseline (0.8 versus 8%, p < 0.05), but was significantly lower than that found during lactic acid loading in nonseptic dogs. None of the vasopressor treatments had a detrimental effect on SLP. These results indicate that LA in sepsis occurs secondary to an increase in splanchnic lactate production that is not related to reduced splanchnic oxygen delivery, as well as to a decrease in hepatic lactate extraction. Effects of different vasoactive agents did not alter either splanchnic lactate production or hepatic lactate extraction in this sepsis model.

Objective: Continuous arteriovenous hemofiltration (CAVH) has been advocated as treatment to remove inflammatory mediators and thereby to improve hemodynamic parameters in sepsis. However, the results obtained with CAVH have been inconsistent. In a canine model of bacteremic Pseudomonas aeruginosa pneumonia, we tested the hypothesis that the time course of the institution of CAVH may be important in obtaining a beneficial treatment effect. Methods: Two protocols were performed in phenobarbital-anesthetized dogs. In the early hemofiltration study (EHS), CAVH for 3 h was initiated 2 h post-pneumonia before mean arterial pressure (MAP) fell. In the late hemofiltration study (LHS), CAVH for 3 h was initiated at 5 h post-pneomonia when a decrease in MAP had already occurred. Hemodynamic measurements included cardiac output (CO), stroke volume (SV), and stroke work (SW). Myocardial depressant activity [filterable cardiodepressant substance (FCS)] found in plasma was assessed by bioassay at each measurement interval. Results: In EHS, after 5 h of sepsis, SW, CO, and SV in the hemofiltered pneumonia group were higher as compared with the nonhemofiltered pneumonia group. In contrast, in LHS, no differences in hemodynamic parameters were found between the two pneumonia groups. In both EHS and LHS, plasma FCS activity was decreased to similar extents by CAVH. Conclusion: These results suggest the time course of institution of CAVH may be important in obtaining a beneficial treatment effect in sepsis.

Computational modeling of reactors and processes has gained wide acceptance in the microelectronics industry. Commercial software are readily available and routinely used at the design stage to reduce design cycle and cost of development. The optoelectronics community can benefit from these advances since many of the processing steps are common, though material chemistries, device configuration and wafer size are different. This paper provides an overview of reactor and process models and specific applications to nitride MOCVD, hydride vapor epitaxy of GaN, SiC CVD and etching of III-V compounds using chlorine chemistry.

A high temperature quasiclassical trajectory (QCT) study is done for the second Zeldovich reaction, C>2 + N —> NO + O to better model its kinetics in hypersonic flows. The thermal rate constants obtained agree well with the available experimental data and various recommended expressions. It is found that vibrational nonequilibrium makes only small differences in the rate of this reaction. The average vibrational energy of NO formed by the 02 -f N —>• NO + O reaction is always higher than the reactant vibrational energy. Also, the product NO vibrational distribution is nonBoltzmann. It is then shown that the vibrational energy disposal due to ©2 + N -> NO + O reaction makes a significant difference in the NO vibrational temperature in re-entry flowfields.

We have isolated an endogenous positive inotropic factor (EPIF) from porcine left heart ventricular tissue, which demonstrated to have only weak digitalis-like properties including the inhibition of myocardial Na+,K+-ATPase. EPIF completely lacks digitalis-like toxicity such as after-contractions in larger doses. In our recent studies, we have demonstrated that EPIF produces a decrease in the amplitude of the post-rest rapid cooling contracture which indicated that EPIF may release Ca2+from the sarcoplasmic reticulum. In the present study, the effects of EPIF were investigated on the Ca2+uptake and release properties of SR enriched membrane vesicles from rat heart. At pH 6.8 and in the presence of oxalate, EPIF dose-dependently inhibited the ATPdependent uptake of Ca2+by SR vesicles. Concentrations as low as 25 ul (in 1 mL uptake medium) of EPIF caused a 45-47% reduction in the uptake of Ca2+within 3-4 min. Increases in EPIF concentration to 50 ul/mL caused additional reduction of only 15-20% in the uptake of Ca2+. Concentrations of 25 ul/mL of EPIF had little or no effects on passive release of actively loaded Ca2+in SR vesicles. On doubling the concentrations to 50 ul/mL EPIF, however, enhanced the release of Ca2+by 25-28% during 1-2 min. and 44-48% after 4 min of incubation of Ca2+loaded vesicles in the release medium. Relatively smaller effects of EPIF on Ca2+release implies that EPIF may mainly lower the uptake of Ca2+in SR. This reduced uptake of Ca2+may be explained by the EPIF-induced inhibition of Ca2+pump.

A new chemistry model is developed for the direct simulation Monte Carlo method (DSMC). This model explicitly includes separate biasing of the reaction cross section to the translational, rotational, and vibrational energies of each collision. The multiple parameter model is calibrated using detailed information on the formation of nitric oxide based on quasi-classical trajectory calculations. The trajectory analysis provides reaction cross sections, and the energy distributions of reactants and products. In the DSMC approach, product energies are assigned by the widely used Borgnakke–Larsen approach. The performance of the new DSMC model is evaluated in terms of reaction cross sections, energy distributions of reacting molecules, energy distributions of nitric oxide molecules formed in the reaction, and overall reaction rate coefficient. In all cases, the new chemistry model gives favorable agreement with the trajectory calculations. The excellent agreement obtained for product energy distributions indicates that the simple Borgnakke–Larsen energy partitioning scheme is a valid approach for this reaction. The new Monte Carlo chemistry model is applied to a hypersonic, low-density, reacting flow of air. By comparison with a previous chemistry model, the new model predicts significantly higher concentrations of nitric oxide. It is also found that nitric oxide molecules are formed in highly nonequilibrium states. Both of these findings are supported by experimental observations.