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To describe the endovascular treatment of a traumatic rupture of a renal artery aneurysm (RAA) in an unstable patient using a stent-graft. A 49-year-old patient presented following trauma to her right chest and flank. The patient was unstable on arrival and following resuscitation, contrast CT angiogram identified a rupture of a left RAA. To occlude the aneurysm, a graft-stent was placed successfully to arrest the haemorrhage. In this case of ruptured RAA, an endovascular approach allowed rapid control of bleeding and preservation of the kidney function, whilst avoiding open surgery and possible nephrectomy.

An increase in train speed generates amplified track deflection. With higher speed, larger strains are induced within the track and subgrade structures. This results in nonlinear behaviour of material properties, particularly the soil stiffness. In railway engineering, it is challenging to deal with these high levels of amplification because the deep wave propagation within the track and underlying soil structures is complicated. Therefore, this paper investigates the influential variables that cause a significant impact on the dynamic amplification of the railway. Four modelling strategies used to generate findings into the problems of railway track dynamics and track-soil nonlinearity. The four types of model are analytical, combined analytical–numerical, 2.5D finite element and 3D finite element. These four models are used to analyse the cases of homogenous half-space soils, homogenous soils above bedrock, layered soils, low-stiffness soil layers and track-soil nonlinearity. The analysis results provide a better understanding of wave propagation characteristics within the subgrade structures. This can be useful for consideration of the design or improvement of railway track structures and earthworks.

The development of high-speed railways is progressing at a very rapid pace worldwide. For example, in the UK, the construction of Phase 1 (225 km) of the HS2 line has now begun, and it will have a maximum operational speed of 360 km/h. In this paper, the performance of a geosynthetic-reinforced soil retaining wall (GRS-RW) is presented for both ballasted track and concrete slab track using full-scale laboratory testing and geotechnical centrifuge modelling. In the full-scale testing, the geo-pavement and railways accelerated fatigue testing (GRAFT II) facility at Heriot-Watt University is used. It operates using six independent hydraulic actuators over three full-size sleepers to simulate the passage of a moving train. The tested GRS-RW structures consist of well-compacted subgrade and a frost protection layer designed to HS2 standards. Results are recorded in terms of deflections, accelerations, total settlement and transient stresses at various locations of the structure model. Whilst the full-scale GRAFT II testing elucidates behaviour of the track and wall, in order to study how the foundation subsoil may interact with the structure, a series of small-scale experiments were carried out in the geotechnical centrifuge by the University of Dundee. By creating small-scale physical models of the GRS-RW and foundation and by increasing gravity, the centrifuge was used to simulate the large induced stresses. Short- and long-term deformations of the wall and foundation were also monitored. Comparisons between the full-scale and centrifuge modelling are presented.

Diabetes contributes to the development of heart failure through various metabolic, structural and biochemical changes. The presence of diabetes increases the risk for the development of cardiovascular disease (CVD), and since the introduction of cardiovascular outcome trials to test diabetic drugs, the importance of improving our understanding of the mechanisms by which diabetes increases the risk for heart failure has come under the spotlight. In addition to the coronary vasculature changes that predispose individuals with diabetes to coronary artery disease, diabetes can also lead to cardiac dysfunction independent of ischaemic heart disease. The hyperlipidaemic, hyperglycaemic and insulin resistant state of diabetes contributes to a perturbed energy metabolic milieu, whereby the heart increases its reliance on fatty acids and decreases glucose oxidative rates. In addition to changes in cardiac energy metabolism, extracellular matrix remodelling contributes to the development of cardiac fibrosis, and impairments in calcium handling result in cardiac contractile dysfunction. Lipotoxicity and glucotoxicity also contribute to impairments in vascular function, cardiac contractility, calcium signalling, oxidative stress, cardiac efficiency and lipoapoptosis. Lastly, changes in protein acetylation, protein methylation and DNA methylation contribute to a myriad of gene expression and protein activity changes. Altogether, these changes lead to decreased cardiac efficiency, increased vulnerability to an ischaemic insult and increased risk for the development of heart failure. This review explores the above mechanisms and the way in which they contribute to cardiac dysfunction in diabetes.

Domestic cats suffer from a range of inherited genetic diseases, many of which display similarities with equivalent human conditions. Developing cellular models for these inherited diseases would enable drug discovery, benefiting feline health and welfare as well as enhancing the potential of cats as relevant animal models for translation to human medicine. Advances in our understanding of these diseases at the cellular level have come from the use of induced pluripotent stem cells (iPSCs). iPSCs can differentiate into virtually any cell type and can be derived from adult somatic cells, therefore overcoming the ethical implications of destroying embryos to obtain embryonic stem cells. No studies, however, report the generation of iPSCs from domestic cats [feline iPSCs (fiPSCs)]. Feline adipose-derived fibroblasts were infected with amphotropic retrovirus containing the coding sequences for human Oct4, Sox2, Klf4, cMyc, and Nanog. Isolated iPSC clones were expanded on inactivated mouse embryonic fibroblasts in the presence of feline leukemia inhibitory factor (fLIF). Retroviral delivery of human pluripotent genes gave rise to putative fiPSC colonies within 5-7 days. These iPS-like cells required fetal bovine serum and fLIF for maintenance. Colonies were domed with refractile edges, similar to mouse iPSCs. Immunocytochemistry demonstrated positive staining for stem cell markers: alkaline phosphatase, Oct4, Sox2, Nanog, and SSEA1. Cells were negative for SSEA4. Expression of endogenous feline Nanog was confirmed by quantitative polymerase chain reaction. The cells were able to differentiate in vitro into cells representative of the three germ layers. These results confirm the first generation of induced pluripotent stem cells from domestic cats. These cells will provide valuable models to study genetic diseases and explore novel therapeutic strategies.

This paper gives new insights into non-linear subgrade behaviour on high speed railway track dynamics. First, a novel semi-analytical model is developed which allows for soil stiffness and damping to dynamically change as a function of strain. The model uses analytical expressions for the railroad track, coupled to a thin-layer element formulation for the ground. Material non-linearity is accounted for using a ‘linear equivalent’ approach which iteratively updates the soil material properties. It is validated using published datasets and in-situ field data. Four case studies are used to investigate non-linear behaviour, each with contrasting subgrade characteristics. Considering an 18 tonne axle load, the critical velocity is significantly lower than the linear case, and rail deflections are up to 30% higher. Furthermore, at speeds close-to, but below the non-linear critical velocity, dynamic amplification is highly sensitive to small increases in train speed. These findings are dependent upon soil material properties, and are important for railway track-earthwork designers because often 70% of the linear critical velocity is used as a design limit. This work shows that designs close to this limit may be still at risk of high dynamic effects, particularly if line speed is increased in the future.

Background Cats with hypertrophic cardiomyopathy (HCM) have decreased left ventricular (LV) longitudinal deformation detected by mitral annular plane systolic excursion (MAPSE) and speckle tracking echocardiography. People with preclinical HCM have decreased systolic LV longitudinal and radial strain (S) and strain rate (SR), with preserved circumferential S and SR. Hypothesis/Objectives Cats with preclinical HCM have decreased systolic LV deformation compared to normal cats. Animals Seventy‐three client‐owned cats with (n = 37) and without (n = 36) preclinical HCM. Methods Retrospective echocardiographic study. Left and right ventricular longitudinal S and SR, LV radial and circumferential S and SR were calculated by STE. Left ventricular mass was also calculated. Correlation between STE variables and LV hypertrophy was determined and receiver‐operating characteristic (ROC) curves were plotted for prediction of HCM. Results Cats with HCM had smaller absolute longitudinal S (−14.8 ± 3.3% vs −19.7 ± 2.7%, P

Railway tracks experience elevated rail deflections when the supporting soil is soft and/or the train speed is greater than approximately 50% of the wave propagation velocity in the track-soil system (i.e. the critical velocity). Such vibrations are undesirable, so soil replacement or soil improvement of the natural soil (or alternatively mini-piles or lime-cement treatment) is often used to increase track-ground stiffness prior to line construction. Although areas of existing soft subgrade might be easily identified on a potential new rail route, it is challenging to determine the type and depth of ground remediation required. Therefore, major cost savings can be made by optimising ground replacement/improvement strategies. This paper presents a numerical railway model, designed for the dynamic analysis of track-ground vibrations induced by high speed rail lines. The model simulates the ground using a thin-layer finite element formulation capable of calculating 3D stresses and strains within the soil during train vehicle passage. The railroad track is modelled using a multi-layered formulation which permits wave propagation in the longitudinal direction, and is coupled with the soil model in the frequency-wavenumber domain. The model is validated using a combination of experimental railway field data, published numerical data and a commercial finite element package. It is shown to predict track and ground behaviour accurately for a range of train speeds. The railway simulation model is computationally efficient and able to quickly assess dynamic, multi-layered soil response in the presence of ballast and slab track structures. Therefore it is well-suited to analysing the effect of different soil replacement strategies on dynamic track behaviour, which is particularly important when close to critical speed. To show this, three soil-embankment examples are used to compare the effect of different combinations of stiffness improvement (stiffness magnitude and remediation depths up to 5 m) on track behaviour. It is found that improvement strategies must be carefully chosen depending upon the track type and existing subgrade layering configuration. Under certain circumstances, soil improvement can have a negligible effect, or possibly even result in elevated track vibration, which may increase long-term settlement. However, large benefits are possible, and if detailed analysis is performed, it is possible to minimise soil improvement depth with respect to construction cost.

Railway lines typically use traditional sloping embankments as the principal means of track support. However, the use of Geosynthetically Reinforced Soil (GRS) systems have gained popularity as alternatives to conventional embankments, particularly for high-speed lines in Japan. This system requires less ground stabilization/improvement and less land take than conventional embankments due to its smaller base area. This research investigates the immediate and long-term settlement behaviour of a Geosynthetically Reinforced Soil with Retaining Wall (GRS-RW) system subject to cyclic loading for two track forms: a concrete slab track and a ballasted track. First, a three-sleeper concrete slab section is constructed at full-scale under controlled laboratory conditions, followed by a ballasted track. Both are supported on a 1.2 m deep subgrade and a frost protection layer in accordance with railway design standards. Two different axle load magnitudes are applied statically, and then cyclically/dynamically, using 6 actuators to replicate moving train axle loads. It is observed that the slab track performs significantly better in terms of elastic and plastic deformation under both static and cyclic loading. Overall, the amplitude of the rail displacement under an individual cycle loading was approximately 25% lower for the slab track and the amplitude of the sleeper displacement on the ballasted track was approximately 6–7 times higher.

Aortic thromboembolism (ATE) occurs in cats with cardiomyopathy and often results in euthanasia due to poor prognosis. However, the underlying predisposing mechanisms leading to left atrial (LA) thrombus formation are not fully characterised. von Willebrand Factor (vWF) is a marker of endothelium and shows increased expression following endothelial injury. In people with poor LA function and LA remodelling, vWF has been implicated in the development of LA thrombosis. In this study we have shown (1) the expression of endocardial vWF protein detected using immunohistofluorescence was elevated in cats with cardiomyopathy, LA enlargement (LAE) and clinical signs compared to cats with subclinical cardiomyopathy and control cats, (2) vWF was present at the periphery of microthrombi and macrothrombi within the LA where they come into contact with the LA endocardium and (3) vWF was integral to the structure of the macrothrombi retrieved from the atria. These results provide evidence for damage of the endocardial endothelium in the remodelled LA and support a role for endocardial vWF as a pro-thrombotic substrate potentially contributing to the development of ATE in cats with underlying cardiomyopathy and LAE. Results from this naturally occurring feline model may inform research into human thrombogenesis.

Objectives The aim of the study was to document whether a proportion of non-diabetic cats with left ventricular hypertrophy (LVH) previously diagnosed with hypertrophic cardiomyopathy (HCM) have elevated circulating insulin-like growth factor 1 (IGF-1) concentrations. Methods A retrospective analysis of residual blood samples obtained at the time of echocardiographic diagnosis of HCM from a population of 60 non-diabetic cats were analysed for circulating IGF-1 concentrations using a validated radioimmunoassay and compared with a control group of 16 apparently healthy cats without LVH. Clinical and echocardiographic data for cats with an IGF-1 level >1000 ng/ml were compared with those with an IGF-1 level Results In total, 6.7% (95% confidence interval 1.8–16.2%) of cats with HCM had an IGF-1 level >1000 ng/ml. The prevalence of an IGF-1 level >1000 ng/ml in the control group was zero. Conclusions and relevance A small proportion of non-diabetic cats previously diagnosed with HCM had an IGF-1 concentration at a level that has been associated with feline hypersomatotropism (fHS) in the diabetic cat population. Further prospective research is required to confirm or refute the presence of fHS in non-diabetic cats with LVH and increased IGF-1.

Número especial Bulletin of Earthquake Engineering, Ag. 2021: S.I. : SOIL-STRUCTURE INTERACTION EFFECTS ON THE DYNAMICS OF STRUCTURES. // Artículo Open Access CCBY 4.0. Disponible online 3 agosto 2021 A novel numerical methodology is presented to solve the dynamic response of railway bridges under the passage of running trains, considering soil–structure interaction. It is advantageous compared to alternative approaches because it permits, (i) consideration of complex geometries for the bridge and foundations, (ii) simulation of stratifed soils, and, (iii) solving the train-bridge dynamic problem at minimal computational cost. The approach uses sub-structuring to split the problem into two coupled interaction problems: the soil–foundation, and the soil–foundation–bridge systems. In the former, the foundation and surrounding soil are discretized with Finite Elements (FE), and padded with Perfectly Match Layers to avoid boundary refections. Considering this domain, the equivalent frequency dependent dynamic stifness and damping characteristics of the soil–foundation system are computed. For the second sub-system, the dynamic response of the structure under railway trafc is computed using a FE model with spring and dashpot elements at the support locations, which have the equivalent properties determined using the frst subsystem. This soil–foundation–bridge model is solved using complex modal superposition, considering the equivalent dynamic stifness and damping of the soil–foundation corresponding to each natural frequency. The proposed approach is then validated using both experimental measurements and an alternative Finite Element–Boundary Element (FE– BE) methodology. A strong match is found and the results discussed Ministerio de Ciencia, Innovación y Universidades PID2019-109622RB Universidad de Sevilla US-126491 Generalitat Valenciana AICO2019/175 Centro Informático Científico de Andalucía (CICA)

In this paper, the performance of different railway structures – ballasted and two types of slab tracks (Rheda system and a ballastless track only constituted by the concrete slab) – is evaluated in terms of stresses and permanent deformations through the selection of some important parameters: wavelength of the unevenness profile, train’s speed, subgrade’s characteristics and spatial variability of the track unevenness profile. The elastodynamic response of 60 case studies is computed through a 2.5D model FEM-PML and the long-term behaviour is assessed by the implementation of a permanent deformation model. The obtained results are analysed through innovative stress and permanent deformation amplification factors in the subgrade layer. This study concludes that the train’s speed and the mechanical properties of the subgrade are the factors that most affect the long-term performance of the subgrade of railway structures, which must be considered for further optimization of the rail track structures., This work was partially carried out under the framework of In2Track2 (Shift2Rail). This work was also supported by FCT - ‘‘Fundação para a Ciência e a Tecnologia”, within ISISE, project UID/ECI/04029/2019. Additionally, by FCT: PD/BD/127814/2016 and FCT/MCTES (PIDDAC): UIDB/04708/2020 of the CONSTRUCT – ‘‘Instituto de I&D em Estruturas e Construções”. The authors are also grateful to the UK Engineering and Physical Sciences Research Council (EPSRC) for funding the LOCORPS project under Grant Number EP/N009207/1

Gas chimneys, fluid-escape pipes, and diffused gas clouds are common geohazards above or below most petroleum reservoirs and in some CO2 storage sites. However, the processes driving the formation of such structures are poorly understood, as are the time scales associated with their growth or their role as long-term preferential fluid-migration pathways in sedimentary basins. We present results from a multidisciplinary study integrating advanced seismic processing techniques with high-resolution simulations of geological processes. Our analyses indicate that time-dependent rock (de)compaction yields ascending solitary porosity waves forming high-porosity and high-permeability vertical chimneys that will reach the surface. The size and location of chimneys depend on the reservoir topology and compaction length. Our simulation results suggest that chimneys in the studied area could have been formed and then lost their connection to the reservoir on a time scale of a few months.

Beam on elastic foundation theory is widely employed when studying railway track behaviour, for applications such as track dynamics, and noise and vibration. At a basic level, the use of a single continuous beam resting on a springs-in-series support is straightforward to implement and computationally efficient. However, it can also be extended to simulate the multi-layered and periodic nature of railway tracks, which typically comprise a variety of components. Further, these track models can also be coupled with both vehicle and subgrade models. Therefore, this paper presents a state-of-the-art technical review of beam on elastic foundation theory, including the exploration of recent advancements in the field. Firstly, a variety of modelling strategies and solution methods employed for the computation of track behaviour are reviewed. These include periodic and semi-periodic modelling approaches. Considerations for extending beam on elastic foundation approaches to include train-track interaction and track-ground interaction are then provided. Finally, using the aforementioned theory, benchmark solutions for three common problem types are given: railway noise, railway track dynamics and railway ground-borne vibration.

Objectives The aim of the study was to evaluate cardiac size and early growth through echocardiographic, body weight (BW), body condition score (BCS), morphometric and biomarker changes in cats followed from 6 to 24 months of age. Methods Twenty-four female European shorthair colony cats were evaluated at birth for BW and at 6, 12, 18 and 24 months of age for BW, BCS, head length (HL) and head width (HW), N-terminal pro B-type natriuretic peptide (NT-proBNP), insulin-like growth factor-1 (IGF-1) and echocardiographic measurements. Results BCS, HW, left ventricular free wall in diastole, left atrium diameter and aortic diameter increased significantly between 6 and 12 months, while BW, HL and interventricular septum in diastole increased significantly between 6, 12 and 18 months, and BW decreased significantly between 18 and 24 months. NT-proBNP decreased significantly between 6 and 12 months. IGF-1 increased significantly between 6 and 12 months but decreased significantly between 12 and 18 months. Conclusions and relevance This study prospectively evaluated changes in echocardiographic measurements, BW, BCS, HL, HW, IGF-1 and NT-proBNP in cats during the first 2 years of life. Results show a comparable change over time for different variables. These findings contribute to the understanding of a possible relationship between cardiac measures and body size from young age through to adulthood.

OBJECTIVE To describe arrhythmias associated with administration of lidocaine in dogs treated for supraventricular tachyarrhythmias. CASE SUMMARIES Four dogs with recent-onset supraventricular tachyarrhythmias: 3 dogs had atrial fibrillation (AF), and 1 had focal atrial tachycardia (FAT), which was thought to be AF at the time of assessment. The substrate of the supraventricular tachyarrhythmia was considered to be due to primary cardiomyopathy in 1 dog, high vagal tone in 2 dogs, and the change in hemodynamics from heavy sedation in 1 dog. Pharmacological cardioversion using lidocaine was only successful in the 2 dogs with vagally mediated AF. In these 2 cases, lidocaine administration resulted in a paroxysmal atrial flutter that was self-limiting and quickly led to sinus rhythm within 10 seconds in 1 dog but did not change over a 5-minute interval and required additional boluses in another dog. In the latter case, the dog showed severe bradycardia for 17.5 seconds prior to achieving sinus rhythm. The 2 unsuccessful cases both developed ventricular arrhythmias shortly after the lidocaine administration, with 1 case degenerating into ventricular fibrillation and cardiac arrest. NEW OR UNIQUE INFORMATION PROVIDED Arrhythmias associated with lidocaine should be considered when treating dogs with supraventricular tachyarrhythmia.

Individuals living with type 1 diabetes mellitus may experience an increased risk of long bone fracture. These fractures are often slow to heal, resulting in delayed reunion or non-union. It is reasonable to theorize that the underlying cause of these diabetes-associated osteopathies is faulty repair dynamics as a result of compromised bone marrow progenitor cell function. Here it was hypothesized that the administration of non-diabetic, human adult bone marrow-derived mesenchymal stromal cells (MSCs) would enhance diabetic fracture healing. Human MSCs were locally introduced to femur fractures in streptozotocin-induced diabetic mice, and the quality of de novo bone was assessed eight weeks later. Biodistribution analysis demonstrated that the cells remained in situ for three days following administration. Bone bridging was evident in all animals. However, a large reparative callus was retained, indicating non-union. &micro, CT analysis elucidated comparable callus dimensions, bone mineral density, bone volume/total volume, and volume of mature bone in all groups that received cells as compared to the saline-treated controls. Four-point bending evaluation of flexural strength, flexural modulus, and total energy to re-fracture did not indicate a statistically significant change as a result of cellular administration. An ex vivo lymphocytic proliferation recall assay indicated that the xenogeneic administration of human cells did not result in an immune response by the murine recipient. Due to this dataset, the administration of non-diabetic bone marrow-derived MSCs did not support fracture healing in this pilot study.

This work presents a simplified method to evaluate building shaking due to arbitrary base excitations, and an example application to railway problems. The model requires minimal computational effort and can be applied to a wide range of footing shapes, thus making it attractive for scoping-type analysis. It uses the soil excitation spectrum at the building footing location as it’s input, and computes the building response at any arbitrary location within it’s 3D structure. To show an application of the model versatility, it is used to compute building response due to a variety of singular railway defects (e.g. switches/crossings). It is however suitable for more general applications including railway problems without defects. The approach is novel because current railway scoping models do not use soil-structure transfer functions combined with free-field response to estimate building vibration by railway defects. First the soil-structure interaction approach is outlined for both rigid and flexible footings. Then it is validated by comparing results against a comprehensive fully-coupled 3D FEM-BEM model. Finally, it is used to analyse the effect of a variety of variables related to railway defects on building response. Local track defects are shown to have a strong influence on building vibrations. Further, vibration levels close to the threshold of human comfort are found in buildings close to the railway line. Overall the new approach allows for the computation of building vibrations accounting for soil-structure interaction, floor amplification and the measured/computed free-field response due to railway traffic using minimal computational effort. Spanish Ministry of Economy and Competitiveness (Ministerio de Economía y Competitividad, España) BIA2016-75042-C2-1-R Spanish Ministry of Education, Culture and Sport, Spain (Ministerio de Educación, Cultura y Deporte, España) PRX18/00115

The para-substituted di-tert-butylfluorosilylbenzene structural motif known as the silicon-fluoride acceptor (SiFA) is a useful tag in the radiochemist's toolkit for incorporating radioactive [

Purpose The purpose of this study was to compare low-dose-rate prostate brachytherapy treatment plans created using three retrospectively applied planning techniques with plans delivered to patients. Methods and Materials Treatment plans were created retrospectively on transrectal ultrasound (TRUS) scans for 26 patients. The technique dubbed 4D Brachytherapy was applied, using TRUS and MRI to obtain prostatic measurements required for the associated webBXT online nomogram. Using a patient's MRI scan to create a treatment plan involving loose seeds was also explored. Plans delivered to patients were made using an intraoperative loose seed TRUS-based planning technique. Prostate V100 (%), prostate V150 (%), prostate D90 (Gy), rectum D0.1cc (Gy), rectum D2cc (Gy), urethra D10 (%), urethra D30 (%), and prostate volumes were measured for each patient. Statistical analysis was used to assess and compare plans. Results Prostate volumes measured by TRUS and MRI were significantly different. Prostate volumes calculated by the webBXT online nomogram using TRUS- and MRI-based measurements were not significantly different. Compared with delivered plans, TRUS-based 4D Brachytherapy plans showed significantly lower rectum D0.1cc (Gy) values, MRI-based 4D Brachytherapy plans showed significantly higher prostate V100 (%) values and significantly lower rectum D0.1cc (Gy), urethra D10 (%), and urethra D30 (%) values, and loose seed MRI-based plans showed significantly lower prostate V100 (%), prostate D90 (Gy), rectum D0.1cc (Gy), rectum D2cc (Gy), urethra D10 (%), and urethra D30 (%) values. Conclusions TRUS-based 4D Brachytherapy plans showed similar dosimetry to delivered plans; rectal dosimetry was superior. MRI can be integrated into the 4D Brachytherapy workflow. The webBXT online nomogram overestimates the required number of seeds.

The para-substituted di-tert-butylfluorosilylbenzene structural motif known as the silicon-fluoride acceptor (SiFA) is a useful tag in the radiochemist's toolkit for incorporating radioactive [18F]fluoride into tracers for use in positron emission tomography. In comparison to conventional radiolabeling strategies, isotopic exchange of fluorine-19 from SiFA with [18F]fluoride is carried out at room temperature and requires minimal reaction participants. The formation of by-products is thus negligible, and purification is greatly simplified. However, while the precursor molecule used for labeling and the final radiolabeled product are isotopically discrete, they are chemically identical and are thus inseparable during purification procedures. The SiFA tag is also susceptible to degradation under the basic conditions arising from the processing and drying of [18F]fluoride. The '4 drop method', wherein only the first 4 drops of eluted [18F]fluoride are used from the solid-phase extraction, reduces the amount of base in the reaction, facilitates lower molar amounts of precursor, and reduces degradation.

In 2016 the first Strategy for Heating and Cooling of the European Union has shown that district heating and cooling networks can integrate renewable energies in an increasingly energy-efficient built environment. At the same time, the heating and cooling sector is probably the most diverse and least mapped component of the European energy system. The aim of the Pan-European Thermal Atlas is to improve the knowledge base for the geographical distribution of heat and cooling demands across Europe. Demand densities of the demanded thermal services themselves, the spatial coherence of these demands, and their location relative to sources of heating greatly affect the economy of district heating schemes compared to individual solutions. The objective is therefore to develop a comprehensive model, which can be used to a) quantify heat demands by density, b) group coherent areas with demands into prospective supply zones, c) produce supply curves for these zones, and d) ultimately calculate local energy mixes on the basis of allocated excess heat as well as renewable energy sources. The developed method spatially disaggregates national demand data to high-resolution geospatial data on urban structures. The resulting atlas allows for an advanced quantitative screening process, which can establish the basis for energy systems analyses relying on geographically explicit information on the heating demand and supply volumes and costs. The present paper presents version 4 of the Pan-European Thermal Atlas, which takes another step towards higher spatial resolution and confidence in comparison to its predecessors, version 1 to 3. For the first time, a 100 m resolution heat atlas of Europe is being presented, which may help describing the heating sector in the required spatial resolution. By means of spatial statistical analyses using ordinary least square linear regressions, multiple spatial inputs such as population, degree of built-up and its derivatives are turned into a coherent model of the urban tissue. Plot ratios form the basis of models of heat demand in single and multi-family residential buildings as well as the service sector. Prospective district heating areas have been delineated, and the resulting zoning of heat supply has been linked to a resource-economic analysis, which allows for cost-supply studies in disaggregated form. The present heat atlas version 4 is now available for 14 countries that altogether represent 90% of the heat demand in the 28 European Union member states. First results are being presented with emphasis on the achieved methodological improvements. Moreover, a newly developed online mapping system is being presented, which will assist in mapping the new geography of heating and cooling demands and supplies.