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56 results on '"Daans, Jasmijn"'

51. Plasmid-based genetic modification of human bone marrow-derived stromal cells: analysis of cell survival and transgene expression after transplantation in rat spinal cord

Author

Ronsyn, Mark W, primary, Daans, Jasmijn, additional, Spaepen, Gie, additional, Chatterjee, Shyama, additional, Vermeulen, Katrien, additional, D'Haese, Patrick, additional, Van Tendeloo, Viggo FI, additional, Van Marck, Eric, additional, Ysebaert, Dirk, additional, Berneman, Zwi N, additional, Jorens, Philippe G, additional, and Ponsaerts, Peter, additional

Published
2007
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53. Labeling of Luciferase/eGFP-expressing bone marrow-derived stromal cells with fluorescent micron-sized iron oxide particles improves quantitative and qualitative multimodal imaging of cellular grafts in vivo.

Author

Vocht, Nathalie, Bergwerf, Irene, Vanhoutte, Greetje, Daans, Jasmijn, Visscher, Geofrey, Chatterjee, Shyama, Pauwels, Patrick, Berneman, Zwi, Ponsaerts, Peter, Linden, Annemie, De Vocht, Nathalie, De Visscher, Geofrey, and Van der Linden, Annemie

Subjects
MESENCHYMAL stem cells, REPORTER genes, CELL transplantation, BIOLUMINESCENCE, MAGNETIC resonance imaging, HISTOLOGY, PROTEIN metabolism, ANIMAL experimentation, BONE marrow, COMPARATIVE studies, CONNECTIVE tissue cells, IRON compounds, LIGHT, RESEARCH methodology, MEDICAL cooperation, MICE, OXIDOREDUCTASES, PARTICLES, RESEARCH, STAINS & staining (Microscopy), THREE-dimensional imaging, EVALUATION research, TRANSPLANTATION of organs, tissues, etc.
Abstract

Purpose: Development of multimodal imaging strategies is currently of utmost importance for the validation of preclinical stem cell therapy studies.Procedures: We performed a combined labeling strategy for bone marrow-derived stromal cells (BMSC) based on genetic modification with the reporter genes Luciferase and eGFP (BMSC-Luc/eGFP) and physical labeling with blue fluorescent micron-sized iron oxide particles (MPIO) in order to unambiguously identify BMSC localization, survival, and differentiation following engraftment in the central nervous system of mice by in vivo bioluminescence (BLI) and magnetic resonance imaging and postmortem histological analysis.Results: Using this combination, a significant increase of in vivo BLI signal was observed for MPIO-labeled BMSC-Luc/eGFP. Moreover, MPIO labeling of BMSC-Luc/eGFP allows for the improved identification of implanted cells within host tissue during histological observation.Conclusions: This study describes an optimized labeling strategy for multimodal stem cell imaging resulting in improved quantitative and qualitative detection of cellular grafts. [ABSTRACT FROM AUTHOR]

Published
2011
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54. Tackling the physiological barriers for successful mesenchymal stem cell transplantation into the central nervous system

Author

De Vocht, Nathalie, Praet, Jelle, Reekmans, Kristien, Le Blon, Debbie, Hoornaert, Chloé, Daans, Jasmijn, Berneman, Zwi, Van Der Linden, Annemie, and Ponsaerts, Peter

Subjects
3. Good health
Abstract

Over the past decade a lot of research has been performed towards the therapeutic use of mesenchymal stem cells (MSCs) in neurodegenerative and neuroinflammatory diseases. MSCs have shown to be beneficial in different preclinical studies of central nervous system (CNS) disorders due to their immunomodulatory properties and their capacity to secrete various growth factors. Nevertheless, most of the transplanted cells die within the first hours after transplantation and induce a neuroinflammatory response. In order to increase the efficacy of MSC transplantation, it is thus imperative to completely characterise the mechanisms mediating neuroinflammation and cell death following MSC transplantation into the CNS. Consequently, different components of these cell death- and neuroinflammation-inducing pathways can be targeted in an attempt to improve the therapeutic potential of MSCs for CNS disorders.

56. Histological characterization and quantification of cellular events following neural and fibroblast(-like) stem cell grafting in healthy and demyelinated CNS tissue.

Author

Praet J, Santermans E, Reekmans K, de Vocht N, Le Blon D, Hoornaert C, Daans J, Goossens H, Berneman Z, Hens N, Van der Linden A, and Ponsaerts P

Subjects
Animals, Cell Culture Techniques, Cell Differentiation, Cell- and Tissue-Based Therapy, Disease Models, Animal, Female, Fibroblasts cytology, Fibroblasts metabolism, Flow Cytometry, Gene Expression, Genes, Reporter, Graft Survival, Immunohistochemistry, Mice, Neural Stem Cells metabolism, Regenerative Medicine, Transgenes, Demyelinating Diseases therapy, Neural Stem Cells cytology, Stem Cell Transplantation
Abstract

Preclinical animal studies involving intracerebral (stem) cell grafting are gaining popularity in many laboratories due to the reported beneficial effects of cell grafting on various diseases or traumata of the central nervous system (CNS). In this chapter, we describe a histological workflow to characterize and quantify cellular events following neural and fibroblast(-like) stem cell grafting in healthy and demyelinated CNS tissue. First, we provide standardized protocols to isolate and culture eGFP(+) neural and fibroblast(-like) stem cells from embryonic mouse tissue. Second, we describe flow cytometric procedures to determine cell viability, eGFP transgene expression, and the expression of different stem cell lineage markers. Third, we explain how to induce reproducible demyelination in the CNS of mice by means of cuprizone administration, a validated mouse model for human multiple sclerosis. Fourth, the technical procedures for cell grafting in the CNS are explained in detail. Finally, an optimized and validated workflow for the quantitative histological analysis of cell graft survival and endogenous astroglial and microglial responses is provided.

Published
2014
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