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Objective: This study aimed to investigate the relationship between increasing levels of assymetric dimethylarginine (ADMA)and ischemiamodified albumin (IMA) in people with early diagnosis of hypertension (HT) and smoking history.Methods: The study included 95 outpatients who visited our hospital. Patients were classified into three groups: group 1, healthy and nonsmokers (n=25); group 2, no smoker patients with newly diagnosed essential HT with LVH (left ventricle hypertrophia) (n=35); group 3, smoking patients with a LVH accompanying newly diagnosed essential HT (n=35). 51% of patients are females; 49% of them are males.The trial was approved by the local ethics committee. Blood samples were analyzed, which were taken after 8 h of fasting. Biochemical parameters such as, C reactive protein (CRP), ADMA, IMA values were recorded. Using echocardiography, cardiac values were recorded.Results: In this study, the first group consisted of 25 patients and second and third group of 35 patients. There were 49 females and 46 males; 70 patients were hypertensive and 6 diabetic. Comparing groups 1 and 2, a significant increase in ADMA was found in group 2. A significant difference was available regarding red cell distribution widht(RDW) and neutrophil lymphocyte ratio (N/L) ratio. Both parameters have increased. Significant differences were found between groups 2 and 3 in terms of ADMA, arginine, albumin, and WBC. While ADMA, white blood cell(WBC), and albumin increased and arginine decreases in group 3.Conclusion: There is an association between ADMA level, smoking, and HT. Patients with HT and smoking history showed increased ADMA level compared with normal. Hypertension and smoking are the causes of increased ADMA, but decreased NO level.

Objective: Extracorporeal Shockwave Lithotripsy (ESWL) is a non-invasive method that is effective at crushing stones in the upper urinary tract. Disturbance of the thiol/disulfide homeostasis, in favor of the disulfide, has been shown to be involved in the disease pathogenesis., Methods: A total of 36 individuals that underwent ESWL had blood samples collected before ESWL (0hrs), 6hrs, and one week after the ESWL. Sera native and total as wells as disulfide amount was measured using an automated method sodium borohydrate (NaBH 4 ) reduction. In addition, Ischemia Modified Albumin (IMA) levels were measured using colorimetric assay method., Results: Native thiol level was reduced at the 6th hour following ESWL compared to baseline. While the ratios of disulfide level, Disulfide/Total Thiol (DTT), Disulfide/Native Thiol (DNT) and IMA level were increased at the 6th hour following ESWL compared to baseline, they were found to be similar with their baseline values at the end of 1st week. Total thiol and native /total thiol did not show any significant change., Conclusions: ESWL treatment disrupts thiol/disulfide homeostasis and the structure of albumin at the acute term. Therefore, it increases protein oxidation and leads to increased oxidative stress. However, this state is transient and returns to normal within the proceeding days.

Objective: To investigate the effect of retrograde intra-renal surgery (RIRS) on kidneys using the myo-inositol oxygenase (MIOX) enzyme. MIOX is a renal tubular-specific novel marker for the early diagnosis of acute kidney injury., Methods: A total of twenty seven individuals that had undergone RIRS to treat kidney stones were included in the study. Biochemical tests were performed on serum samples collected immediately before RIRS (hour 0) and at the 6th and 24th hours after the surgery., Results: The creatinine value at hour 6 was lower than the baseline (hour 0) value (p = 0.0305). Cystatin C at hour 6 was lower than the value measured at hour 24 (p = 0.0142). Similarly, MIOX was lower at hour 6 compared to hour 24 (p = 0.0214). MIOX/creatinine at hour 6 was lower than the value calculated at hour 24 (p = 0.0348). The basal values of MIOX and creatinine were found to have a positive correlation (correlation coefficient r = 0.5946, p = 0.0035)., Conclusions: Similar to the serum creatinine, the serum MIOX level provides information about kidney functions. RIRS was confirmed to be a safe procedure for the treatment of acute kidney injury with no negative effects on the kidneys.