51. miRNAs Plasma Profiles in Vascular Dementia: Biomolecular Data and Biomedical Implications
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Cinzia Di Pietro, R. S. Spada, Paolo Bosco, Angelo Giuseppe Condorelli, Lucia Tamburello, Davide Barbagallo, Marina Scalia, Marco Ragusa, Mariangela Tornitore, Cristina Barbagallo, Maurizio Elia, and Michele Purrello
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0301 basic medicine ,Alzheimer’s dementia ,Biology ,non-coding-RNAs plasma profiles ,Bioinformatics ,lcsh:RC321-571 ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,liquid biopsies ,0302 clinical medicine ,Text mining ,Downregulation and upregulation ,Vascular dementia, Alzheimer’s dementia ,microRNA ,parasitic diseases ,medicine ,TaqMan ,Dementia ,cardiovascular diseases ,Vascular dementia ,lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry ,Original Research ,Receiver operating characteristic ,business.industry ,biomarkers ,vascular dementia ,Plasma levels ,medicine.disease ,030104 developmental biology ,Noncoding RNAs Plasma Profiles ,biological phenomena, cell phenomena, and immunity ,business ,030217 neurology & neurosurgery ,Neuroscience - Abstract
Vascular dementia (VaD) is a pathogenetically heterogeneous neuropsychiatric syndrome, mainly characterized by cognitive impairment. Among dementias, it is second by incidence after Alzheimer’s dementia (AD). VaD biomolecular bases have been poorly characterized, but vascular-linked factors affecting the CNS and its functions are generally hypothesized to perform a major role, together with cardiovascular and immunological factors. miRNAs, which perform critically important biomolecular roles within cell networks, are also found in biological fluids as circulating miRNAs (cmiRNAs). We hypothesized that differentially expressed (DE) cmiRNAs in plasma from VaD patients could be applied to diagnose VaD through liquid biopsies; these profiles also could allow to start investigating VaD molecular bases. By exploiting TaqMan Low-Density Arrays and single TaqMan assays, miR-10b*, miR29a-3p, and miR-130b-3p were discovered and validated as significantly downregulated DE cmiRNAs in VaD patients compared to unaffected controls (NCs). These miRNAs also were found to be significantly downregulated in a matched cohort of AD patients, but miR-130b-3p levels were lower in AD than in VaD. A negative correlation was detected between miR-29a and miR-130b expression and cognitive impairment in VaD and AD, respectively. Receiver operating characteristic curves demonstrated that decreased plasma levels of miR-10b*, miR29a-3p, and miR-130b-3p allow to discriminate VaD and AD patients from NCs. Furthermore, the concurrent downregulation of both miR-10b* and miR-130b-3p in VaD showed an area under the curve (AUC) of 0.789 (p
- Published
- 2016
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