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Research Framework: Existing studies agree that the work involved in managing debts and their consequences is mostly undertaken by women. We know that this debt work involves more deprivation and sacrifice, but we do not know its effects on women’s wealth.Objectives : This article identifies processes explaining the link between debt work and the decline of women’s wealth.Methodology: This article is based on 44 semi-structured interviews conducted in Switzerland with overindebted couples with or without children as part of two debt-related research projects funded by the Swiss National Science Foundation (2016-2022).Results: We show that the predominance of women in debt work can be explained by the fact that it is not just financial and administrative work. It is experienced as parental and conjugal care work. Concern for and preservation of family members’ well-being, especially children, leads women to invest their own assets. Perceived as a means of doing gender in a situation of over-indebtedness by acting as a “good” mother and spouse, this work and the inequalities it produces are not called into question.Conclusion : The financialization of everyday life implies new forms of financial work, such as debt work, which reinforce wealth inequalities within the family, with men’s wealth and that of their children being preserved to the prejudice of women’s wealth. In this regard, the financial assets of each family members have different meanings, with women’s wealth becoming an adjustment variable in the event of economic difficulties, enabling family status and good family relations to be maintained.Contribution : By focusing on the financial burdens carried by women, this article offers a new approach to understanding the gendered processes involved in the constitution of wealth inequalities, which until now have focused mainly on gendered access to money and inheritance.

The eriochrome black T dye belongs to the azo dye class and is often used as an indicator in complexometric titrations and as a fiber dye in industrial processes. It constitutes a direct threat to human health and the environment due to its toxic and carcinogenic nature. Separating this dye from wastewater is essential before its release into water bodies. In this work, the adsorption capacity of eriochrome black T in aqueous solution conferred on the chemically modified or unmodified coffee husk fibers was evaluated. Adsorption experiments were performed in triplicate and batch. The results obtained were adjusted and presented as adsorption isotherms using the Langmuir-Freundlich model. The mass value of adsorbed dye was 483.9 mg ± 17.35 mg and 547.2 mg ± 22.98 mg of eriochrome black T per gram of unmodified and chemically modified coffee husk, respectively, indicating great potential of coffee husk as an adsorbent for the removal of anionic azo dyes in aqueous solution.

Abstract is missing (Short communication)

Summary: Macrophages are major host cells for the protozoan Leishmania parasite. Depending on their activation state, they either contribute to the detection and elimination of Leishmania spp. or promote parasite resilience. Here, we report that the activation of the transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) in macrophages plays a pivotal role in the progression of Leishmania infantum infection by controlling inflammation and redox balance of macrophages. We also highlight the involvement of the NOX2/reactive oxygen species (ROS) axis in early Nrf2 activation and, subsequently, prostaglandin E2 (PGE2)/EP2r signaling in the sustenance of Nrf2 activation upon infection. Moreover, we establish a ferroptosis-like process within macrophages as a cell death program of L. infantum and the protective effect of Nrf2 in macrophages against L. infantum death. Altogether, these results identify Nrf2 as a critical factor for the susceptibility of L. infantum infection, highlighting Nrf2 as a promising pharmacological target for the development of therapeutic approaches for the treatment of visceral leishmaniasis.

Abstract Background The objective of this study was to identify and qualify, by means of a three-dimensional kinematic analysis, the postures and movements of obstetricians during a simulated forceps birth, and then to study the association of the obstetricians’ experience with the technique adopted. Method Fifty-seven volunteer obstetricians, 20 from the Limoges and 37 from the Poitiers University hospitals, were included in this multi-centric study. They were classified into 3 groups: beginners, intermediates, and experts, beginners having performed fewer than 10 forceps deliveries in real conditions, intermediates between 10 and 100, and experts more than 100. The posture and movements of the obstetricians were recorded between December 2020 and March 2021 using an optoelectronic motion capture system during simulated forceps births. Joint angles qualifying these postures and movements were analysed between the three phases of the foetal traction. These phases were defined by the passage of a virtual point associated with the forceps blade through two anatomical planes: the mid-pelvis and the pelvic outlet. Then, a consolidated ascending hierarchical classification (AHC) was applied to these data in order to objectify the existence of groups of similar behaviours. Results The AHC distinguished four different postures adopted when crossing the first plane and three different traction techniques. 48% of the beginners adopted one of the two raised posture, 22% being raised without trunk flexion and 26% raised with trunk flexion. Conversely, 58% of the experts positioned themselves in a “chevalier servant” posture (going down on one knee) and 25% in a “squatting” posture before initiating traction. The results also show that the joint movement amplitude tends to reduce with the level of expertise. Conclusion Forceps delivery was performed in different ways, with the experienced obstetricians favouring postures that enabled observation at the level of the maternal perineum and techniques reducing movement amplitude. The first perspective of this work is to relate these different techniques to the traction force generated. The results of these studies have the potential to contribute to the training of obstetricians in forceps delivery, and to improve the safety of women and newborns.

Abstract A novel methylation class, “neuroepithelial tumor, with PLAGL1 fusion” (NET-PLAGL1), has recently been described, based on epigenetic features, as a supratentorial pediatric brain tumor with recurrent histopathological features suggesting an ependymal differentiation. Because of the recent identification of this neoplastic entity, few histopathological, radiological and clinical data are available. Herein, we present a detailed series of nine cases of PLAGL1-fused supratentorial tumors, reclassified from a series of supratentorial ependymomas, non-ZFTA/non-YAP1 fusion-positive and subependymomas of the young. This study included extensive clinical, radiological, histopathological, ultrastructural, immunohistochemical, genetic and epigenetic (DNA methylation profiling) data for characterization. An important aim of this work was to evaluate the sensitivity and specificity of a novel fluorescent in situ hybridization (FISH) targeting the PLAGL1 gene. Using histopathology, immunohistochemistry and electron microscopy, we confirmed the ependymal differentiation of this new neoplastic entity. Indeed, the cases histopathologically presented as “mixed subependymomas-ependymomas” with well-circumscribed tumors exhibiting a diffuse immunoreactivity for GFAP, without expression of Olig2 or SOX10. Ultrastructurally, they also harbored features reminiscent of ependymal differentiation, such as cilia. Different gene partners were fused with PLAGL1: FOXO1, EWSR1 and for the first time MAML2. The PLAGL1 FISH presented a 100% sensitivity and specificity according to RNA sequencing and DNA methylation profiling results. This cohort of supratentorial PLAGL1-fused tumors highlights: 1/ the ependymal cell origin of this new neoplastic entity; 2/ benefit of looking for a PLAGL1 fusion in supratentorial cases of non-ZFTA/non-YAP1 ependymomas; and 3/ the usefulness of PLAGL1 FISH.

Here we present a detailed description of different geomorphic features to complement the Maps of Geohazard Features of the Ionian Calabrian Margin produced by the Magic project (Marine Geohazard along Italian Coasts). Some of the most striking features we imaged are sources of widespread and recurrent geohazards. These include multiple coastal landslides, failure scars along open slopes, shelf-indenting retrogressive canyon headwalls and active fluid venting structures, that we investigated by integrating regional high-resolution multibeam sonar and sub-bottom profiling data. The main triggers and predisposing factors for the marine geohazards that we identify in our study area include frequent seismic activity, the rapid uplift of the margin since 1 Ma and the presence of Messinian evaporites at depth. Large-scale gravity-driven movements and the incipient retrogressive canyon headwalls are of particular concern, as they are located just a few hundred meters from the coast, where critical infrastructures and densely populated urban centers are situated, and also where high-resolution geophysical data are often lacking. Overall, our study provides a key reference for more detailed follow-up studies to foster a better understanding of marine geohazard occurrences. The insights provided are critical for planning monitoring programs and for the protection of coastal settlements and marine infrastructures along the Calabrian Ionian margin.

ABSTRACTWe present the characterization of geohazard-related features of the Ligurian Sea with the map of its physiographic domains (1:250,000 scale) and five maps (1:100,000 scale) of the morphological and morpho-bathymetric elements. These were realized in the framework of the MaGIC (Marine Geohazard along Italian Coasts) project, promoted by the Italian Civil Protection Department. The characterization of submarine geohazards along continental margins, together with mapping of marine geological features are critical for the management of coastal risks. Multibeam bathymetric data combined with high-resolution 2D reflection seismic data, allowed to identify and map the main tectono-sedimentary features related to mass movements along the Ligurian continental margin. A dense grid of seismic profiles allowed to improve the comprehension of the margin structure as well as its recent tectonic activity providing new insights concerning mass wasting processes and active tectonics relationships to plan further, focalized investigations in areas of maximum risk.

Collagens are the most abundant proteins in the body and among the most biosynthetically complex. A molecular ensemble of over 20 endoplasmic reticulum resident proteins participates in collagen biosynthesis and contributes to heterogeneous post-translational modifications. Pathogenic variants in genes encoding collagens cause connective tissue disorders, including osteogenesis imperfecta, Ehlers-Danlos syndrome, and Gould syndrome (caused by mutations in COL4A1 and COL4A2), and pathogenic variants in genes encoding proteins required for collagen biosynthesis can cause similar but overlapping clinical phenotypes. Notably, pathogenic variants in lysyl hydroxylase 3 (LH3) cause a multisystem connective tissue disorder that exhibits pathophysiological features of collagen-related disorders. LH3 is a multifunctional collagen-modifying enzyme; however, its precise role(s) and substrate specificity during collagen biosynthesis has not been defined. To address this critical gap in knowledge, we generated LH3 KO cells and performed detailed quantitative and molecular analyses of collagen substrates. We found that LH3 deficiency severely impaired secretion of collagen α1α1α2(IV) but not collagens α1α1α2(I) or α1α1α1(III). Amino acid analysis revealed that LH3 is a selective LH for collagen α1α1α2(IV) but a general glucosyltransferase for collagens α1α1α2(IV), α1α1α2(I), and α1α1α1(III). Importantly, we identified rare variants that are predicted to be pathogenic in the gene encoding LH3 in two of 113 fetuses with intracranial hemorrhage-a cardinal feature of Gould syndrome. Collectively, our findings highlight a critical role of LH3 in α1α1α2(IV) biosynthesis and suggest that LH3 pathogenic variants might contribute to Gould syndrome.

Background: Non-muscle invasive bladder cancer (NMIBC) patients are affected by a high risk of recurrence. The topography of collagen fibers represents a hallmark of the neoplastic extracellular microenvironment. Objective: Assess the topographic change associated with different stages of bladder cancer (from neoplastic lesions to bona fide tumor) and whether those changes favour the development of NMIBC. Design, Setting, and Participants: Seventy-one clinical samples of urothelial carcinoma at different stages were used. Topographic changes preceding tumor onset and progression were evaluated in the rat bladder cancer model induced by nitrosamine (BBN), a bladder-specific carcinogen. The preclinical model of actinic cystitis was also used in combination with BBN. Validated hematoxylin-eosin sections were used to assess the topography of collagen fibrils associated with pre-tumoral steps, NMIBC, and MIBC. Findings: Linearization of collagen fibers was higher in Cis and Ta vs. dysplastic urothelium, further increased in T1 and greatest in T2 tumors. In the BBN preclinical model, an increase in the linearization of collagen fibers was established since the beginning of inflammation, such as the onset of atypia of a non-univocal nature and dysplasia, and further increased in the presence of the tumor. Linearization of collagen fibers in the model of actinic cystitis was associated with earlier onset of BBN-induced tumor. Conclusions: The topographic modification of the extracellular microenvironment occurs during the inflammatory processes preceding and favoring the onset of bladder cancer. The topographic reconfiguration of the stroma could represent a marker for identifying and treating the non-neoplastic tissue susceptible to tumor recurrence.

Abstract Climate change is projected to increase the frequency and intensity of extreme heat events, and may increase humidity levels, leading to coupled thermal and hydric stress. However, how humidity modulates the impacts of heat stress on species and their interactions is currently unknown. Using an insect host‐parasitoid interaction: the Indian meal moth, Plodia interpunctella, and its endoparasitoid wasp, Venturia canescens, we investigated how humidity interacted with heat stress duration, applied at different host developmental stages, to affect life history traits. Hosts parasitized as 4th instar larvae and unparasitized hosts were maintained in high‐ (60.8% RH) or low‐humidity (32.5% RH) at constant 28°C. They were then exposed to a 38°C thermal stress with a duration of 0 (no heat stress), 6 or 72 h in either the 4th or 5th host instar. Neither humidity nor heat stress duration affected emergence of unparasitized hosts, but increasing heat stress duration during the 4th instar decreased parasitoid emergence irrespective of humidity. When applied during the 5th instar, increasing heat duration decreased parasitoid emergence under low humidity, but no effect of heat stress was found under high humidity. Moreover, experiencing longer heat stress in the 4th instar increased host larval development time and decreased body size under high humidity, but this effect differed under low humidity; increasing heat duration in the 5th instar decreased parasitoid body sizes only under low humidity. Larval stage and heat stress duration directly affected parasitized host survival time, with a concomitant indirect reduction of parasitoid sizes. We show that humidity modifies key life history responses of hosts and parasitoids to heat stress in species‐specific ways, highlighting the potential importance of humidity in regulating host‐parasitoid interactions and their population dynamics. Finally, we emphasize that interactions between environmental stressors need to be considered in climate change research.

Summary: Background: Nomegestrol acetate (NOMAC) is a synthetic potent progestogen. This study aimed to assess the risk of intracranial meningioma associated with the prolonged use of NOMAC. Methods: Observational cohort study using SNDS data (France). Women included had ≥ one dispensing of NOMAC between 2007 and 2017 (no dispensing in 2006). Exposure was defined as a cumulative dose >150 mg NOMAC within six months after first dispensing. A control group of women (cumulative dose ≤150 mg) was assembled. The outcome was surgery (resection or decompression) or radiotherapy for one or more intracranial meningioma(s). Poisson models assessed the relative risk (RR) of meningioma. Findings: In total, 1,060,779 women were included in the cohort (535,115 in the exposed group and 525,664 in the control group). The incidence of meningioma in the two groups was 19.3 and 7.0 per 100,000 person-years, respectively (age-adjusted RRa = 2.9 [2.4–3.7]). The RRa for a cumulative dose of more than 6 g NOMAC was 12.0 [9.9–16.0]. In the event of treatment discontinuation for at least one year, the risk of meningioma was identical to that in the control group (RRa = 1.0 [0.8–1.3]). The location of meningiomas in the anterior and middle part of the skull base was more frequent with exposure to NOMAC. Interpretation: We observed a strong dose-dependent association between prolonged use of NOMAC and the risk of intracranial meningiomas. These results are comparable to those obtained for cyproterone acetate, although the magnitude of the risk is lower. It is now recommended to stop using NOMAC if a meningioma is diagnosed. Funding: The French National Health Insurance Fund (Cnam) and the French National Agency for Medicines and Health Products Safety (ANSM) via the Health Product Epidemiology Scientific Interest Group EPI-PHARE.

IntroductionWithin adipose tissue (AT), different macrophage subsets have been described, which played pivotal and specific roles in upholding tissue homeostasis under both physiological and pathological conditions. Nonetheless, studying resident macrophages in-vitro poses challenges, as the isolation process and the culture for extended periods can alter their inherent properties.MethodsStroma-vascular cells isolated from murine subcutaneous AT were seeded on ultra-low adherent plates in the presence of macrophage colony-stimulating factor. After 4 days of culture, the cells spontaneously aggregate to form spheroids. A week later, macrophages begin to spread out of the spheroid and adhere to the culture plate.ResultsThis innovative three-dimensional (3D) culture method enables the generation of functional mature macrophages that present distinct genic and phenotypic characteristics compared to bone marrow–derived macrophages. They also show specific metabolic activity and polarization in response to stimulation, but similar phagocytic capacity. Additionally, based on single-cell analysis, AT-macrophages generated in 3D culture mirror the phenotypic and functional traits of in-vivo AT resident macrophages.DiscussionOur study describes a 3D in-vitro system for generating and culturing functional AT-resident macrophages, without the need for cell sorting. This system thus stands as a valuable resource for exploring the differentiation and function of AT-macrophages in vitro in diverse physiological and pathological contexts.

We study the matrix completion problem: an underlying [Formula omitted] matrix P is low rank, with incoherent singular vectors, and a random [Formula omitted] matrix A is equal to P on a (uniformly) random subset of entries of size dn. All other entries of A are equal to zero. The goal is to retrieve information on P from the observation of A. Let [Formula omitted] be the random matrix where each entry of A is multiplied by an independent [Formula omitted]-Bernoulli random variable with parameter 1/2. This paper is about when, how and why the non-Hermitian eigen-spectra of the matrices [Formula omitted] and [Formula omitted] captures more of the relevant information about the principal component structure of A than the eigen-spectra of [Formula omitted] and [Formula omitted]. We show that the eigenvalues of the asymmetric matrices [Formula omitted] and [Formula omitted] with modulus greater than a detection threshold are asymptotically equal to the eigenvalues of [Formula omitted] and [Formula omitted] and that the associated eigenvectors are aligned as well. The central surprise is that by intentionally inducing asymmetry and additional randomness via the [Formula omitted] matrix, we can extract more information than if we had worked with the singular value decomposition (SVD) of A. The associated detection threshold is asymptotically exact and is non-universal since it explicitly depends on the element-wise distribution of the underlying matrix P. We show that reliable, statistically optimal but not perfect matrix recovery, via a universal data-driven algorithm, is possible above this detection threshold using the information extracted from the asymmetric eigen-decompositions. Averaging the left and right eigenvectors provably improves estimation accuracy but not the detection threshold. Our results encompass the very sparse regime where d is of order 1 where matrix completion via the SVD of A fails or produces unreliable recovery. We define another variant of this asymmetric principal component analysis procedure that bypasses the randomization step and has a detection threshold that is smaller by a constant factor but with a computational cost that is larger by a polynomial factor of the number of observed entries. Both detection thresholds allow to go beyond the barrier due to the well-known information theoretical limit [Formula omitted] for exact matrix completion found in the literature., Author(s): Charles Bordenave [sup.1] , Simon Coste [sup.2] , Raj Rao Nadakuditi [sup.3] Author Affiliations: (1) grid.5399.6, 0000 0001 2176 4817, Institut de mathématiques de Marseille, Aix-Marseille Université, , 39 [...]