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Objectives: To evaluate the influence of photobiomodulation with infrared laser (IRL) in the rat pulp tissue after bleaching, considering the immunolabeling of interleukin (IL)-23 and hypoxia-inducible factor (HIF)-1α. Methodology: The right and left molars of forty rats were divided into groups: Control – with placebo gel and Bleached – with 35% hydrogen peroxide (H2O2). Half of the rats received one IRL application on both sides, establishing a split-mouth design, which resulted in 4 groups with 20 hemi-maxillae each: Control, Bleach, IRL, and Bleached-IRL. Rats (n=10) from each group were euthanized, at 2- and 30-days mark, and the pulp tissue was evaluated using inflammation and immunolabeling scores. Wilcoxon and Mann-Whitney statistical tests were performed (p0.05). HIF-1α was more evident at the 2-days mark in the Bleached group, without significant difference with the Bleached-IRL (p>0.05). The difference was observed between the bleached and control groups, without immunolabeling (p

Palatability and swallowability in the pediatric population are perceived as true challenges in the oral administration of medication. Pediatric patients have high sensitivity to taste and reduced ability to take solid dosage forms, which can often lead to a poor therapeutic compliance. It is crucial to find new strategies to simplify the oral administration of drugs to children. The present paper reports the development of a new hydrogel vehicle adapted to the pediatric population. Several polymers with similar properties were selected and adjustments were made to obtain the desired characteristics of the final product. The developed formulations were studied for organoleptic properties, rheology, mucoadhesion properties, preservative efficacy, and stability. Physical and chemical compatibilities between the vehicle and several drugs/medicines, at the time of administration, were also studied. Six final formulations with different polymers, odor, and color were chosen, and no known interactions with medications were observed. The proposed new oral vehicles are the first sugar-free vehicle hydrogels designed to make the intake of oral solid forms a more pleasant and safer experience for pediatric patients., This research was funded by FCT (Fundação para a Ciência e a Tecnologia) through iMed.ULisboa UID/DTP/04138/2020 and UIDB/04138/2020). Joana Marto is financed through FCT, I.P., under the Scientific Employment Stimulus—Institutional Call (CEECINST/00145/2018).

Made available in DSpace on 2021-06-25T12:36:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01. Added 1 bitstream(s) on 2021-07-15T15:24:33Z : No. of bitstreams: 1 S1983-46322021000100344.pdf: 1756051 bytes, checksum: a445a5ffbb6a119d52d29dfd8aebf0ea (MD5) Objective: To investigate the tissue response and the biomineralization ability of CER prepared with epoxy resin or water compared to Mineral Trioxide Aggregate (MTA). Material and Methods: Polyethylene tubes containing materials or empty tubes for control were inserted into the subcutaneous tissues of 30 rats. After 7, 15, 30, 60, and 90 days, the rats were killed and the tubes were removed for analysis using hematoxylin-eosin staining, von Kossa staining, and under polarized light. Inflammation was graded through a score system; the thickness of the fibrous capsule was classified as thin or thick; the biomineralization ability was recorded as present or absent. The results were statistically analyzed using the Kruskal-Wallis test (p0.05). After 30, 60, and 90 days, mild inflammation, and a thin fibrous capsule were observed in all groups (p>0.05). Conclusion: All materials had structures positive for von Kossa and birefringent to polarized light. CER epoxy resin showed biocompatibility and biomineralization similar to CER water and MTA. Sao Paulo State Univ, Aracatuba Sch Dent, Dept Prevent & Restorat Dent, Discipline Endodont, Aracatuba, SP, Brazil Sao Paulo State Univ, Sch Engn, Dept Phys & Chem, Ilha Solteira, SP, Brazil Univ Fed Alagoas, Sch Dent, Dept Odontol, Maceio, Alagoas, Brazil Sao Paulo State Univ, Aracatuba Sch Dent, Dept Prevent & Restorat Dent, Discipline Endodont, Aracatuba, SP, Brazil Sao Paulo State Univ, Sch Engn, Dept Phys & Chem, Ilha Solteira, SP, Brazil

Made available in DSpace on 2022-05-01T06:02:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-10-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Aim: To evaluate the effect of excessive caffeine intake on the inflammation/resorption processes associated with periapical periodontitis (PP) in rats. Methodology: Sixteen Wistar rats were used. Periapical periodontitis was induced in the four first molars in each animal. The animals were arranged into two groups: control (C)—rats with periapical periodontitis; and caffeine (CAF)—rats with periapical periodontitis under caffeine administration protocol. The CAF animals received 10 mg/100 g of body weight/day of caffeine via gavage starting fifteen days before PP induction and continuing for thirty more days until euthanasia. On the 30th day, the animals were euthanized and the jaws removed for microcomputed tomography, histological and immunohistochemical analysis for RANKL, OPG, TRAP, IL-10, TNF-⍺ and IL-1β. The Mann–Whitney test was performed for nonparametric data, and Student's t test was performed for parametric data, using p

As amidoximas possuem sao amplamente aplicaveis e importantes para os setores industriais, biologicos e farmacologicos. No campo dos compostos bioativos, as amidoximas destacam-se por apresentarem acao frentes a diferentes celulas patologicas, alem de atuarem como pro-farmacos de amidinas em meio biologico. Contudo estudos envolvendo amidoximas estruturalmente mais simples sao pouco comuns, especialmente a respeito do perfil toxicologico desses compostos. Em concordância com a importância desses compostos o presente trabalho objetivou sintetizar a 4-Bromobenzamidoxima e verificar sua toxicidade frente as larvas de Artemia salina Leach. A sintese da respectiva amidoxima foi realizada a partir da correspondente arilnitrila comercialmente disponivel. O composto alvo foi caracterizado utilizando as tecnicas espectroscopicas usuais (IV, RMN 1 H e 13 C). O bioensaio toxicologico do composto alvo com a A. salina apresentou uma toxicidade acentuada com CL 50 igual a 38,549 μg/mL, valor obtido atraves do software estatistico do software POLO-PC. Esse resultado motiva a utilizacao da 4-Bromobenzamidoxima em outros testes biologicos, incluindo em celulas ou ate mesmo animais, na busca de encontrar um novo farmaco.

Objective: Evaluate and compare the antimicrobial efficacy of four different commercially available children’s toothpastes on bacteria growth inhibition from oral microbiota of young children. Material and Method: The toothpastes tested contained: 1100 ppm sodium fluoride, xylitol and 750 ppm sodium fluoride, xylitol and 500 ppm sodium fluoride, and xylitol and triclosan. Twenty 18 to 30 month-old children were selected. Nonstimulated saliva samples were collected and inoculated on brain heart infusion (BHI) agar. Toothpaste dilutions were applied into the agar wells and incubated at 37ºC for 24 hours in microaerophilic conditions. Saline and 0.12% chlorhexidine were used as controls. Antimicrobial activity was determined in duplicate by agar-well diffusion technique. Bacterial growth inhibition zones were recorded in mm. Statistical analysis was performed using ANOVA and Tukey's test (p

Banana as a climacteric fruit has a relatively short shelf-life period and thus, technologies that decrease the metabolism and the triggering of the maturation process are extremely necessary on its postharvest conservation. However, the consequences of these technologies on quality attributes are unknown. Therefore, we evaluate the effects of 1-Methylcyclopropene associated with low density polyethylene bags on physical and chemical attributes in the postharvest of banana fruits. Bananas were treated with different concentration of 1-Methylcyclopropene as 0, 50, 100, 150 and 200 ηL.L-1 under refrigeration and harvest in five different times after treatment. Further, fruits treated with 50 ŋL.L-¹ showed a more advanced stage of ripening after the 25 days of storage. Altogether, our results suggest 1-MCP is an effective treatment to control sugar and starch metabolism in banana and its efficiency is directly dependent of storage temperature. Additionally we identify interestingly correlation with skin color changes with sugars and starch content, which can indicate its potentiality of the fitted equations for prediction of central metabolism of bananas non-destructively using ‘hue angle and chrome’ value.

Objetivo: O objetivo deste trabalho foi o de identificar quais são os principais cimentos endodônticos que promovem a alteração de cor dental, além de mostrar quais outros caminhos podem ser seguidos, a fim de evitar o prejuízo estético do dente tratado. Metodologia: Foi realizada uma busca em inglês dos artigos através da base de dados PubMed, MedLine, Cochrane e Lilacs, no período de 2005 a 2020. Resultados: Observou-se que a maioria dos cimentos testados promoveram alterações de cor, especialmente materiais à base de óxido de zinco-eugenol e agregado trióxido mineral, quando aplicados em dentes anteriores, devido à estrutura delgada desse dente. Isso ocorre devido a componentes químicos presentes nesses materiais. Os componentes biocerâmicos são os que promovem menores alterações, quando comparados aos outros materiais citados neste estudo. Considerações Finais: A adequada limpeza da câmara pulpar é fundamental para evitar a descoloração dental após o tratamento endodôntico. O clareamento dental interno e a utilização do agente adesivo dentinário podem ser alternativas para melhorar a estética devido ao escurecimento dental provocado pelos obturadores endodônticos.

Endodontics has gained emphasis in the scientific community in recent years due to the increase in clinical and in animal models studies focused on endodontic medicine, which aims to evaluate the interrelationship between systemic and periapical tissues pathological conditions. These studies have shown that systemic changes can boost the pathogenesis of endodontic infection, favoring its development and progression. A contrary relationship is reported in numerous studies that affirm the potential of endodontic infection to trigger systemic damage and may lead to the worsening of pre-existing pathologies. Recently, the potential of filling materials to develop systemic changes such as neurological alterations had been evaluated, also showing that systemic diseases can negatively influence tissue responses to filling materials after endodontic treatment. Despite advances in endodontic medicine studies, there are still gaps in knowledge on the mechanisms of interactions between apical periodontitis (AP) and systemic diseases and much research to be done. In this sense, this critical narrative literature review aimed to show the evolution of studies in endodontic medicine to help the endodontist to know the role of systemic diseases in the pathogenesis of AP and the possible interference in the repair of periapical tissues after endodontic treatment, as well as to evidence the systemic complications that can be triggered or aggravated in the presence of endodontic infection.

Objective: To evaluate the pulp tissue of rat molars after pulpotomies with mineral trioxide aggregate (MTA), Biodentine TM (BDT) and calcium hydroxide (CH) mixed with sterile saline solution (24 hours, 72 hours, 7 days and 15 days), through correlating MPO activity with active neutrophils and MMP8 activity with tissue remodeling . Material and Methods: Thirty-eight Wistar rats were randomly distributed into groups (control, I (MTA gray), II (BDT), and III (CH)) and subdivided according to the study period of 24 hours, 72 hours, 7 days or 15 days after pulpotomy. MMP8 activity was assessed through fluorescence technique, and MPO activity was determined using the MPO assay. Results: A gradual decrease of MPO and MM8 activity occurred in the group MTA over the experimental periods (p

Made available in DSpace on 2021-06-25T10:51:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01. Added 1 bitstream(s) on 2021-07-15T15:23:40Z : No. of bitstreams: 1 S1678-77572021000100403.pdf: 5527338 bytes, checksum: 9b28ab8a5ce07b81fc69125cf7ac3eaf (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objective: The aim of this study was to evaluate whether probiotics multi-strain formula affects the development of apical periodontitis (AP) induced in rats. Methodology: 16 Wistar rats were divided in two groups (n=8): rats with AP fed with regular diet (Control-C (CG)); rats with AP, fed with regular diet and supplemented with multi-strain formula (one billion colony-forming units (CFU)): GNC Probiotic Complex (PCG) (Lactobacillus acidophilus, Lactobacillus salivaris, Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium bifidum, Bifidobacterium animalis subs. lactis and Streptococcus thermofilus). AP was induced in the upper and lower first molars by dental pulp exposure to the oral environment. PCG was administered orally through gavage for 30 days during the AP development. After this period the animals were euthanized and the mandibles were removed and processed for histologic analysis, and immunochemical assays for interleukin (IL)-6, IL-10, IL-1β, RANKL, OPG, and TRAP. The Mann–Whitney U test and Student’s t test were performed (P.05). The number of mature osteoclasts (TRAP-positive multinucleated cells) was lower in PCG group when compared to the CG (P

Objective: The aim of this study was to evaluate the periodontal status of teeth indicated for undergoing endodontic treatment. Materials and Methods: Two hundred and nine teeth were evaluated using probing depth tests at three vestibular and three palatine/lingual sites before the patients underwent treatment. Teeth that presented up to 3-mm probing depth were considered as healthy. Those that presented at least one site with probing depth >3 mm were considered periodontally compromised. The data were statistically analyzed by means of applying the Chi-square test, with a level of significance of 5%. Results: Of the total of 209 teeth evaluated, 40 (19.10%) presented periodontal compromise. There was statistically significant difference related to the compromised teeth of patients of the female gender (22.80%) compared with teeth of patients of the male gender (12.30%) (P < 0.05). Conclusions: Fewer than 20% of the teeth evaluated presented a periodontal compromise. Patients of the female gender presented a higher number of periodontally compromised teeth than patients of the male gender.

Guava is a typically tropical fruit highly perishable with a short shelf-life due to intense metabolic activity after harvested. In attempt to minimize the problems related to the postharvest, we evaluated the physiochemical characteristics and antioxidant system in guava fruits under chitosan coating at concentrations of 1%, 2%, and 3% stored at 25 °C during 96 h. The chitosan suppressed the respiratory rate, fresh weight loss, firmness and skin color with delay in the degradation of chlorophyll. In the treatment with 2% and 3% of chitosan in the solid soluble content and ascorbic acid were reduced; retarded the loss of titratable acidity during 96 h after treatment. These treatment induced significant decreases in the phenylalanine ammonia-lyase activity and significantly increases of peroxidase Activity. Our results suggest that chitosan effectively prolongs the quality attributes in guava fruits after harvesting due to increases in the antioxidant processes, delaying the ripening during room temperature of storage.

The aim of this study was to evaluate the clinical, radiographic and histological outcomes of the dentin-pulp complex from primary molars after pulpotomy with mineral trioxide aggregate (MTA) and 15.5% ferric sulfate (FS). Thirty-one primary molars were randomly allocated into MTA or FS groups. Clinical and radiographic evaluations were recorded at 3-, 6-, 12- and 18-month follow-up. Teeth at the regular exfoliation period were extracted and processed for histological analysis. Clinical and radiographic data were tested by statistical analysis (p≤0.01). Histological outcomes were analyzed descriptively. All of the treated teeth presented clinical success over the experimental periods. Both groups exhibited 100% of radiographic success at 3, 6 and 12 months. At the 18-month follow-up, one tooth from FS group presented inter-radicular radiolucency (p>0.01). Histologically, the treated teeth presented pulp vitality and absence of inflammatory infiltrate into the connective tissue. Only MTA group showed hard tissue barrier surrounded by odontoblasts over the pulp stumps. Both MTA and 15.5% FS are effective for pulpotomies of primary teeth. Although MTA is considered the first-choice material, FS may be a suitable alternative when treatment cost is an issue. Resumo O objetivo deste estudo foi avaliar os resultados clínicos, radiográficos e histológicos do complexo dentino-pulpar de molares decíduos após pulpotomia com agregado trióxido mineral (MTA) e sulfato férrico (SF) 15,5%. Trinta e um molares decíduos foram alocados aleatoriamente nos grupos MTA ou SF. As avaliações clínicas e radiográficas foram realizadas aos 3, 6, 12 e 18 meses de acompanhamento. Os dentes no período regular de esfoliação foram extraídos e processados para análise histológica. Os dados clínicos e radiográficos foram analisados estatisticamente (p≤0,01). O resultados histológicos foram analisados descritivamente. Todos os dentes tratados apresentaram sucesso clínico ao longo dos períodos experimentais. Ambos os grupos exibiram 100% de sucesso radiográfico aos 3, 6 e 12 meses. Aos 18 meses de acompanhamento, um dente do grupo SF apresentou radiolucidez interradicular (p>0,01). Histologicamente, os dentes tratados apresentaram vitalidade pulpar e ausência de infiltrado inflamatório no tecido conjuntivo. Somente o grupo MTA mostrou barreira de tecido duro rodeada por odontoblastos sobre os cotos pulpares. MTA e SF 15,5% são eficazes para pulpotomias de dentes decíduos. Embora o MTA seja considerado o material de primeira escolha, o SF pode ser uma alternativa adequada quando o custo do tratamento é um problema.

Os traumatismos dentários constituem um problema de saúde de alta frequência em crianças e adultos jovens, e estão associados a consequências psicológicas, funcionais e ainda estéticas. A necrose pulpar é uma das principais consequências após o trauma dental, implicando, dessa forma, na falha do desenvolvimento radicular dos dentes permanentes recém erupcionados, os quais em razão disso permanecem com paredes finas e ápice aberto. O objetivo deste trabalho foi descrever uma opção de tratamento que permite, ao mesmo tempo, o fechamento do ápice radicular e o reforço das paredes apicais em dentes imaturos que apresentem status de necrose pulpar após esses sofrerem trauma. Foi utilizado como técnica a apicificação com aplicação única de hidróxido de cálcio. O presente trabalho permitiu concluir que a apicificação com hidróxido de cálcio é uma alternativa de baixo custo, facilmente aplicável na rotina clínica e que apresenta altas taxas de sucesso no tratamento de dentes imaturos traumatizados.

O tratamento de dentes com rizogênese incompleta e necrose pulpar representa um grande desafio para a terapia endodôntica, principalmente pela fragilidade das paredes radiculares desses dentes. Uma terapêutica que pode ser realizada é a revascularização pulpar com o objetivo de substituir estruturas danificadas, incluindo estruturas dentinárias e radiculares, bem como células do complexo dentino-pulpar com um tecido que permita a continuação da formação da raiz em espessura e tamanho. Esses procedimentos demonstraram ser capazes de promover o desenvolvimento continuado da raiz, aumentando assim a espessura e a resistência das raízes anteriormente finas e propensas à fratura. Este trabalho tem como objetivo realizar, através de uma revisão da literatura, um estudo sobre a revascularização pulpar e sua importância clínica no tratamento de dentes acometidos por necrose pulpar e cuja formação apical ainda não se completou. Para este estudo, foi realizado um levantamento na plataforma PubMed, em que foram incluídos artigos publicados entre 2004 e 2021. A revascularização pulpar pode ser considerada como uma alternativa promissora como tratamento para dentes com rizogênese incompleta e necrose pulpar, tendo vantagens potenciais em relação ao tradicional processo de apicificação.

A dor pós-operatória ao tratamento endodôntico é de origem multifatorial e pode afetar a qualidade de vida do paciente. Uma variedade de técnicas e combinações podem alcançar o controle da dor durante os procedimentos endodônticos. Ter conhecimento desses fatores guia o dentista em sua capacidade de aumentar a confiança do paciente, conduzir o tratamento de acordo com os fatores de risco para a dor e tomar medidas para reduzir os sintomas. Dessa forma, o objetivo deste trabalho foi analisar as evidências atualmente disponíveis sobre o tema dor pós-operatória ao tratamento endodôntico. Para sua concretização foi realizado um levantamento na plataforma PubMed e incluídos artigos de revisão sistemática publicados entre 2016 e 2021. Os resultados deste estudo mostraram que a administração da medicação pré-operatória, a manutenção da patência apical, a agitação da substância irrigante com dispositivos automatizados e o uso de crioterapia intracanal são favoráveis na prevenção e/ou redução dos sintomas pós-operatórios. No entanto, faz-se necessário novos ensaios clínicos randomizados bem desenhados para melhor esclarecimento de alguns fatores relacionados à algesia após o tratamento endodôntico.

A fratura radicular horizontal pode ser definida como aquela que envolve dentina, cemento, ligamento periodontal e polpa, compreendendo de 1,2 a 7% dos traumas na dentição permanente. Afeta normalmente a região de dentes anteriores, e geralmente cicatriza-se espontaneamente, dependendo da vitalidade pulpar. O diagnóstico é realizado através de exame clínico, teste de sensibilidade pulpar e exame radiográfico. Além disso, a Tomografia Computadorizada de Feixe Cônico tem sido utilizada de forma a auxiliar no diagnóstico e prognóstico, devido a reprodução de imagens tridimensionais, permitindo visualizar de forma exata a localização e trajeto da linha de fratura. Este estudo relata um caso clínico de trauma dental causado por queda de própria altura. A paciente apresentava fratura radicular horizontal no elemento 21, localizada em terço médio, que foi confirmada através do exame radiográfico. Como tratamento imediato, foi realizado o reposicionamento e esplintagem. Para a confirmação da localização exata da fratura e se houve o processo de cicatrização, foi realizada imagens de tomografia computadorizada de feixe cônico após 30 dias do trauma, pôde-se visualizar a fratura com trajeto oblíquo cicatrizada por tecido calcificado. Dessa forma, optou-se por acompanhamento da fratura radicular horizontal com tempo de controle de 15 dias, 30 dias, 60 dias, 2 anos e 5 anos. Permitindo, assim, que o dente seja preservado na cavidade bucal, sem intervenções.

Objectives This study evaluated smear layer removal by different chemical solutions used with or without ultrasonic activation after post preparation. Materials and Methods Forty-five extracted uniradicular human mandibular premolars with single canals were treated endodontically. The cervical and middle thirds of the fillings were then removed, and the specimens were divided into 9 groups: G1, saline solution (NaCl); G2, 2.5% sodium hypochlorite (NaOCl); G3, 2% chlorhexidine (CHX); G4, 11.5% polyacrylic acid (PAA); G5, 17% ethylenediaminetetraacetic acid (EDTA). For the groups 6, 7, 8, and 9, the same solutions used in the groups 2, 3, 4, and 5 were used, respectively, but activated with ultrasonic activation. Afterwards, the roots were analyzed by a score considering the images obtained from a scanning electron microscope. Results EDTA achieved the best performance compared with the other solutions evaluated regardless of the irrigation method (p < 0.05). Conclusions Ultrasonic activation did not significantly influence smear layer removal.

Objective: This study evaluated the leakage volume of 1% Rhodamine B which received or not an internal waterproofing material. The hypothesis is that the waterproofing reduces microleakage. Material and Methods: Forty-two human teeth were selected for this study. After access, preparation, and filling of root canals, the teeth were divided into 3 groups: GI – sealed with Cimpat Blanc®, without intraradicular post/core preparation; GII - sealed with Cimpat Blanc®, with intraradicular post/core preparation, and GIII – sealed with Cimpat Blanc®, with intraradicular post/core preparation and internal waterproofing material with ethyl cyanoacrylate. T-test was used to analyze difference between initial and final weight and there was difference (p < 0.05). To analyze infiltration of the colorant, the volume was the comparison variable. Results: Shapiro Wilk- test was applied and showed normal distribution of the variables. ANOVA (p = 0.0013 / p

This study aims to know the main factors related to work processes that interfere on the effectiveness of the humanization policy in primary health care in Brazil. In the methodological aspect, it is a qualitativedescriptive study from a Systematic Review. A data collection was based on the selection of articles from surveys conducted in the Virtual Health Library. Were found that most studies identify important factors that jeopardize the execution of the National Policy of Humanization, such as: humanization design, working conditions, and health management in primary care. It is concluded that the main impairing factors in the effectiveness of this policy were the working conditions and an improper health management conducts.

Enulosides, carbohydrate derivatives containing an α,β-unsaturated carbonyl unit, were designed and obtained in high yields and isomeric purity. All synthesized compounds exhibited antitumoral activity in micromolar range against four tested tumor cells lines, being the best results observed for HL-60 cells. These compounds open new possibilities to prepare an array of more active, site-specific or selective antitumor agents. 2016 Elsevier Ltd. All rights reserved.

Objective: To evaluate sleep quality and excessive daytime sleepiness in undergraduate dentistry students. Material and Methods: This research is characterized as an observational study of transversal type, having analysed undergraduate students in dentistry from a public university in the State of Pernambuco, Brazil. The sample consisted of 325 students enrolled between the 1st and 10th academic semester. Sleep quality was evaluated using the Pittsburgh Sleep Quality Index (PSQI), while excessive daytime sleepiness was analysed through the Epworth Sleepiness Scale (ESS) . The t-Student and Mann-Whitney tests were used for the numerical values, with the F-test (ANOVA) or the Kruskal-Wallis test being used to compare more than two categories. Results: It was observed that 71.1% of students presented a poor sleep quality, with more than half of the students exhibiting excessive daytime sleepiness (58.1%). A relationship between sleep quality and the academic semester was verified. However, gender and age were not associated with sleep quality or with excessive daytime sleepiness . Conclusion: A high prevalence of poor sleep quality and excessive daytime sleepiness was observed among undergraduate students in dentistry. The data suggest that the undergraduate degree in Dentistry can influence the students’ quality of sleep, regardless of age or gender.

Made available in DSpace on 2020-12-10T20:09:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01. Added 1 bitstream(s) on 2021-07-15T15:25:12Z : No. of bitstreams: 1 S1678-77572020000100460.pdf: 4776031 bytes, checksum: c1bf4403f9557901fbf3f37581ccd600 (MD5) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Aim: To evaluate the cytotoxicity, biocompatibility and mineralization capacity of BIO-C PULPO, and MTA. Methodology: L929 fibroblasts were cultured and MTT assay was used to determine the material cytotoxicity on 6, 24, and 48 h. A total of 30 male rats (Wistar) aged between 4 and 6 months, weighing between 250 and 300 g were used. Polyethylene tubes containing BIO-C PULPO, MTA, and empty tubes were implanted into dorsal connective tissue. After the experimental periods (7, 15, 30, 60, and 90 days) the tubes were histologically analyzed using hematoxylin-eosin (H&E), immunolabeling of IL-1 beta and TNF-alpha, and von Kossa staining, or without staining for polarized light analysis. The average number of inflammatory cells was quantified; the mineralization assessment was determined by the area marked in pmt and semiquantitative immunolabeling analyses of IL-1 beta and TNF-alpha were performed. Then, data underwent statistical analysis with a 5% significance level. Results: It was observed that BIO-C PULPO and MTA presented cytocompatibility at 6, 24, and 48 similar or higher than control for all evaluated period. On periods 7 and 15 days, BIO-C PULPO was the material with the highest number of inflammatory cells (p0.05). No statistical differences were found between Control, BIO-C PULPO, and MTA for immunolabeling of IL-1 beta and TNF-alpha in the different periods of analysis (p

Attempts to improve the ascorbate (AsA) content of plants are still dealing with the limited understanding of why exists a wide variability of this powerful anti-oxidant molecule in different plant sources, species and environmental situations. In plant mitochondria, the last step of AsA synthesis is catalyzed by the enzyme L-galactone-1,4-lactone dehydrogenase (L-GalLDH). By using GalLDH-RNAi silencing plant lines, biochemical and proteomic approaches, we here discovered that, in addition to accumulate this antioxidant, mitochondria synthesize AsA to down-regulate the respiratory activity and the cellular energy provision. The work reveals that the AsA synthesis pathway within mitochondria is a branched electron transfer process that channels electrons towards the alternative oxidase, interfering with conventional electron transport. It was unexpectedly found that significant hydrogen peroxide is generated during AsA synthesis, which affects the AsA level. The induced AsA synthesis shows proteomic alterations of mitochondrial and extra-mitochondrial proteins related to oxidative and energetic metabolism. The most identified proteins were known components of plant responses to high light acclimation, programmed cell death, oxidative stress, senescence, cell expansion, iron and phosphorus starvation, different abiotic stress/pathogen attack responses and others. We propose that changing the electron flux associated with AsA synthesis might be part of a new mechanism by which the L-GalLDH enzyme would adapt plant mitochondria to fluctuating energy demands and redox status occurring under different physiological contexts., Instituto de Fisiología Vegetal

Palatability in the paediatric population is perceived as a true challenge once their ability to take solid oral dosage forms is far more reduced than adults, which often can lead to a poor therapeutic compliance. In Portugal, there is not any vehicle to aid the administration of drugs to paediatric patients so, the aim of this project was to develop a hydrogel vehicle to facilitate the intake of tablets, capsules and other types of oral medicines in children. This research work allowed us to demonstrate how rheological methodologies were crucial to optimize some physical properties of the vehicle, thought to be critical to improve the patient’s experience during the oral intake of a drug.

Made available in DSpace on 2020-12-12T02:06:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-08-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Aim: To evaluate the relationship between systemic administration of probiotics and inflammation/resorption processes associated with apical periodontitis (AP) in a rat model. Methodology: Twenty-four male Wistar rats were used. AP was induced in the mandibular left/right first molars. The animals were arranged into three groups: Control, Lactobacillus rhamnosus and L. acidophilus. Probiotics were orally administered via gavage (109 colony-forming units (CFU) diluted in 5 mL of water) for 30 days during the development of AP. On the 30th day, blood was collected to analyse the calcium, phosphorus and alkaline phosphatase concentrations in plasma. Then, the animals were euthanized and the jaws removed for micro-computed tomography and immune-histopathological analysis for receptor activator of NF-κB ligand (RANKL), osteoprotegerin (OPG) and tartrate-resistant acid phosphatase (TRAP). After the Shapiro–Wilk test of normality, the Kruskal–Wallis followed by Dunn’s test was performed for nonparametric data, and analysis of variance followed by the Tukey test was performed for parametric data (P 0.05). The level of alkaline phosphatase was significantly higher in the groups that consumed probiotics (P