Author: "Clayton, Aled" - Searchworks@Jio Institute Digital Library Search Results

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51. Membrane Radiolabelling of Exosomes for Comparative Biodistribution Analysis in Immunocompetent and Immunodeficient Mice - A Novel and Universal Approach

Author: Faruqu, Farid N., Wang, Julie Tzu-Wen, Xu, Lizhou, McNickle, Luke, Chong, Eden Ming-Yiu, Walters, Adam, Gurney, Mark, Clayton, Aled, Smyth, Lesley A., Hider, Robert, Sosabowski, Jane, and Al-Jamal, Khuloud T.
Subjects: NANOCAPSULES, exosomes, Research & Experimental Medicine, radiolabelling, DELIVERY, Drug Delivery Systems, Cell Line, Tumor, Animals, Tissue Distribution, 1112 Oncology and Carcinogenesis, CANCER CELLS, biodistribution, LIPOSOMAL ALENDRONATE, Drug Carriers, Science & Technology, B16BL6-DERIVED EXOSOMES, SIRNA, Cell Membrane, IN-VITRO, IN-111 TROPOLONE, Mice, Inbred C57BL, CONJUGATION, Medicine, Research & Experimental, Isotope Labeling, drug delivery, DTPA, Administration, Intravenous, Life Sciences & Biomedicine, Research Paper
Abstract: Extracellular vesicles, in particular exosomes, have recently gained interest as novel drug delivery vectors due to their biological origin and inherent intercellular biomolecule delivery capability. An in-depth knowledge of their in vivo biodistribution is therefore essential. This work aimed to develop a novel, reliable and universal method to radiolabel exosomes to study their in vivo biodistribution.Methods: Melanoma (B16F10) cells were cultured in bioreactor flasks to increase exosome yield. B16F10-derived exosomes (ExoB16) were isolated using ultracentrfugation onto a single sucrose cushion, and were characterised for size, yield, purity, exosomal markers and morphology using Nanoparticle Tracking Analysis (NTA), protein measurements, flow cytometry and electron microscopy. ExoB16 were radiolabelled using 2 different approaches - intraluminal labelling (entrapment of 111Indium via tropolone shuttling); and membrane labelling (chelation of 111Indium via covalently attached bifunctional chelator DTPA-anhydride). Labelling efficiency and stability was assessed using gel filtration and thin layer chromatography. Melanoma-bearing immunocompetent (C57BL/6) and immunodeficient (NSG) mice were injected intravenously with radiolabelled ExoB16 (1x1011 particles/mouse) followed by metabolic cages study, whole body SPECT-CT imaging and ex vivo gamma counting at 1, 4 and 24 h post-injection.Results: Membrane-labelled ExoB16 showed superior radiolabelling efficiency and radiochemical stability (19.2 ± 4.53 % and 80.4 ± 1.6 % respectively) compared to the intraluminal-labelled exosomes (4.73 ± 0.39 % and 14.21 ± 2.76 % respectively). Using the membrane-labelling approach, the in vivo biodistribution of ExoB16 in melanoma-bearing C57Bl/6 mice was carried out, and was found to accumulate primarily in the liver and spleen (~56% and ~38% ID/gT respectively), followed by the kidneys (~3% ID/gT). ExoB16 showed minimal tumour i.e. self-tissue accumulation (~0.7% ID/gT). The membrane-labelling approach was also used to study ExoB16 biodistribution in melanoma-bearing immunocompromised (NSG) mice, to compare with that in the immunocompetent C57Bl/6 mice. Similar biodistribution profile was observed in both C57BL/6 and NSG mice, where prominent accumulation was seen in liver and spleen, apart from the significantly lower tumour accumulation observed in the NSG mice (~0.3% ID/gT).Conclusion: Membrane radiolabelling of exosomes is a reliable approach that allows for accurate live imaging and quantitative biodistribution studies to be performed on potentially all exosome types without engineering parent cells.
Published: 2019

52. Considerations towards a roadmap for collection, handling and storage of blood extracellular vesicles

Author: Clayton, Aled, Boilard, Eric, Buzas, Edit I, Cheng, Lesley, Falcón-Perez, Juan Manual, Gardiner, Chris, Gustafson, Dakota, Gualerzi, Alice, Hendrix, An, Hoffman, Andrew, Jones, Jennifer, Lässer, Cecilia, Lawson, Charlotte, Lenassi, Metka, Nazarenko, Irina, O'Driscoll, Lorraine, Pink, Ryan, Siljander, Pia R-M, Soekmadji, Carolina, Wauben, Marca, Welsh, Joshua A, Witwer, Ken, Zheng, Lei, Nieuwland, Rienk, Clayton, Aled, Boilard, Eric, Buzas, Edit I, Cheng, Lesley, Falcón-Perez, Juan Manual, Gardiner, Chris, Gustafson, Dakota, Gualerzi, Alice, Hendrix, An, Hoffman, Andrew, Jones, Jennifer, Lässer, Cecilia, Lawson, Charlotte, Lenassi, Metka, Nazarenko, Irina, O'Driscoll, Lorraine, Pink, Ryan, Siljander, Pia R-M, Soekmadji, Carolina, Wauben, Marca, Welsh, Joshua A, Witwer, Ken, Zheng, Lei, and Nieuwland, Rienk
Abstract: There is an increasing interest in exploring clinically relevant information that is present in body fluids, and extracellular vesicles (EVs) are intrinsic components of body fluids ("liquid biopsies"). In this report, we will focus on blood. Blood contains not only EVs but also cells, and non-EV particles including lipoproteins. Due to the high concentration of soluble proteins and lipoproteins, blood, plasma and serum have a high viscosity and density, which hampers the concentration, isolation and detection of EVs. Because most if not all studies on EVs are single-centre studies, their clinical relevance remains limited. Therefore, there is an urgent need to improve standardization and reproducibility of EV research. As a first step, the International Society on Extracellular Vesicles organized a biomarker workshop in Birmingham (UK) in November 2017, and during that workshop several working groups were created to focus on a particular body fluid. This report is the first output of the blood EV work group and is based on responses by work group members to a questionnaire in order to discover the contours of a roadmap. From the answers it is clear that most respondents are in favour of evidence-based research, education, quality control procedures, and physical models to improve our understanding and comparison of concentration, isolation and detection methods. Since blood is such a complex body fluid, we assume that the outcome of the survey may also be valuable for exploring body fluids other than blood.
Published: 2019

53. Considerations towards a roadmap for collection, handling and storage of blood extracellular vesicles

Author: LS Celbiologie-Algemeen, dB&C I&I, Clayton, Aled, Boilard, Eric, Buzas, Edit I, Cheng, Lesley, Falcón-Perez, Juan Manual, Gardiner, Chris, Gustafson, Dakota, Gualerzi, Alice, Hendrix, An, Hoffman, Andrew, Jones, Jennifer, Lässer, Cecilia, Lawson, Charlotte, Lenassi, Metka, Nazarenko, Irina, O'Driscoll, Lorraine, Pink, Ryan, Siljander, Pia R-M, Soekmadji, Carolina, Wauben, Marca, Welsh, Joshua A, Witwer, Ken, Zheng, Lei, Nieuwland, Rienk, LS Celbiologie-Algemeen, dB&C I&I, Clayton, Aled, Boilard, Eric, Buzas, Edit I, Cheng, Lesley, Falcón-Perez, Juan Manual, Gardiner, Chris, Gustafson, Dakota, Gualerzi, Alice, Hendrix, An, Hoffman, Andrew, Jones, Jennifer, Lässer, Cecilia, Lawson, Charlotte, Lenassi, Metka, Nazarenko, Irina, O'Driscoll, Lorraine, Pink, Ryan, Siljander, Pia R-M, Soekmadji, Carolina, Wauben, Marca, Welsh, Joshua A, Witwer, Ken, Zheng, Lei, and Nieuwland, Rienk
Published: 2019

54. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Author: Théry, Clotilde, Witwer, Kenneth W., Aikawa, Elena, Alcaraz, Maria Jose, Anderson, Johnathon D., Andriantsitohaina, Ramaroson, Antoniou, Anna, Arab, Tanina, Archer, Fabienne, Atkiin-Smith, Georgia K., Ayre, D. Craig, Bach, Jean-Marie, Bachurski, Daniel, Baharvand, Hossein, Balaj, Leonora, Baldacchino, Shawn, Bauer, Natalie N., Baxter, Amy A., Bebawy, Mary, Beckham, Carla, Zavec, Apolonija Bedina, Benmoussa, Abderrahim, Berardi, Anna C., Bergese, Paolo, Bielska, Ewa, Blenkiron, Cherie, Bobis-Wozowicz, Sylwia, Boilard, Eric, Boireau, Wilfrid, Bongiovanni, Antonella, Borràs, Francesc E., Bosch, Steffi, Boulanger, Chantal M., Breakefield, Xandra, Breglio, Andrew M., Brennan, Meadhbh Á, Brigstock, David R., Brisson, Alain, Broekman, Marike L.D., Bromberg, Jacqueline F., Bryl-Górecka, Paulina, Buch, Shilpa, Buck, Amy H., Burger, Dylan, Busatto, Sara, Buschmann, Dominik, Bussolati, Benedetta, Buzás, Edit I., Byrd, James Bryan, Camussi, Giovanni, Carter, David R.F., Caruso, Sarah, Chamley, Lawrence W., Chang, Yu-Ting, Chen, Chichen, Chen, Shuai, Cheng, Lesley, Chin, Andrew R., Clayton, Aled, Clerici, Stefano P., Cocks, Alex, Cocucci, Emanuele, Coffey, Robert J., Cordeiro-da-Silva, Anabela, Couch, Yvonne, Coumans, Frank A.W., Coyle, Beth, Crescitelli, Rossella, Criado, Miria Ferreira, D’ Souza-Schorey, Crislyn, Das, Saumya, Chaudhuri, Amrita Datta, de Candia, Paola, De Santana Junior, Eliezer F., De Wever, Olivier, del Portillo, Hernando A., Demaret, Tanguy, Deville, Sarah, Devitt, Andrew, Dhondt, Bert, Di Vizio, Dolores, Dieterich, Lothar C., Dolo, Vincenza, Dominguez Rubio, Ana Paula, Dominici, Massimo, Dourado, Mauricio R., Driedonks, Tom A.P, Duarte, Filipe V., Duncan, Heather M., Eichenberger, Ramon M., Ekström, Karin, El Andaloussi, Samir, Elie-Caille, Celine, Erdbrügger, Uta, Falcón-Pérez, Juan M., Fatima, Farah, Fish, Jason E., Flores-Bellver, Miguel, Försönits, András, Frelet-Barrand, Annie, Fricke, Fabia, Fuhrmann, Gregor, Gabrielsson, Susanne, Gámez-Valero, Ana, Gardiner, Chris, Gärtner, Kathrin, Gaudin, Raphael, Gho, Yong Song, Giebel, Bernd, Gilbert, Caroline, Gimona, Mario, Giusti, Ilaria, Goberdhan, Deborah C.I., Görgens, André, Gorski, Sharon M., Greening, David W., Gross, Julia Christina, Gualerzi, Alice, Gupta, Gopal N., Gustafson, Dakota, Handberg, Aase, Haraszti, Reka A., Harrison, Paul, Hegyesi, Hargita, Hendrix, An, Hill, Andrew F., Hochberg, Fred H., Hoffmann, Karl F., Holder, Beth, Holthofer, Harry, Hosseinkhani, Baharak, Hu, Guoku, Huang, Yiyao, Huber, Veronica, Hunt, Stuart, Ibrahim, Ahmed Gamal-Eldin, Ikezu, Tsuneya, Inal, Jameel M., Isin, Mustafa, Ivanova, Alena, Jackson, Hannah K., Jacobsen, Soren, Jay, Steven M., Jayachandran, Muthuvel, Jenster, Guido, Jiang, Lanzhou, Johnson, Suzanne M., Jones, Jennifer C., Jong, Ambrose, Jovanovic-Talisman, Tijana, Jung, Stephanie, Kalluri, Raghu, Kano, Shin-ichi, Kaur, Sukhbir, Kawamura, Yumi, Keller, Evan T., Khamari, Delaram, Khomyakova, Elena, Khvorova, Anastasia, Kierulf, Peter, Kim, Kwang Pyo, Kislinger, Thomas, Klingeborn, Mikael, Klinke II, David J., Kornek, Miroslaw, Kosanović, Maja M., Kovács, Árpád Ferenc, Krämer-Albers, Eva-Maria, Krasemann, Susanne, Krause, Mirja, Kurochkin, Igor V., Kusuma, Gina D., Kuypers, Sören, Laitinen, Saara, Langevin, Scott M., Languino, Lucia R., Lannigan, Joanne, Lässer, Cecilia, Laurent, Louise C., Lavieu, Gregory, Lázaro-Ibáñez, Elisa, Lay, Soazig Le, Lee, Myung-Shin, Lee, Yi Xin Fiona, Lemos, Debora S., Lenassi, Metka, Leszczynska, Aleksandra, Li, Isaac T.S., Liao, Ke, Libregts, Sten F., Ligeti, Erzsebet, Lim, Rebecca, Lim, Sai Kiang, Linē, Aija, Linnemannstöns, Karen, Llorente, Alicia, Lombard, Catherine A., Lorenowicz, Magdalena J., Lörincz, Ákos M., Lötvall, Jan, Lovett, Jason, Lowry, Michelle C., Loyer, Xavier, Lu, Quan, Lukomska, Barbara, Lunavat, Taral R., Maas, Sybren L.N., Malhi, Harmeet, Marcilla, Antonio, Mariani, Jacopo, Mariscal, Javier, Martens-Uzunova, Elena S., Martin-Jaular, Lorena, Martinez, M. Carmen, Martins, Vilma Regina, Mathieu, Mathilde, Mathivanan, Suresh, Maugeri, Marco, McGinnis, Lynda K., McVey, Mark J., Meckes, Jr., David G., Meehan, Katie L., Mertens, Inge, Minciacchi, Valentina R., Möller, Andreas, Jørgensen, Malene Møller, Morales-Kastresana, Aizea, Morhayim, Jess, Mullier, François, Muraca, Maurizio, Musante, Luca, Mussack, Veronika, Muth, Dillon C., Myburgh, Kathryn H., Najrana, Tanbir, Nawaz, Muhammad, Nazarenko, Irina, Nejsum, Peter, Neri, Christian, Neri, Tommaso, Nieuwland, Rienk, Nimrichter, Leonardo, Nolan, John P., Nolte-’ t Hoen, Esther N.M., Hooten, Nicole Noren, O’Driscoll, Lorraine, O’Grady, Tina, O’Loghlen, Ana, Ochiya, Takahiro, Olivier, Martin, Ortiz, Alberto, Ortiz, Luis A., Osteikoetxea, Xabier, Ostegaard, Ole, Ostrowski, Matias, Park, Jaesung, Pegtel, D. Michiel, Peinado, Hector, Perut, Francesca, Pfaffl, Michael W., Phinney, Donald G., Pieters, Bartijn C.H., Pink, Ryan C., Pisetsky, David S., von Strandmann, Elke Pogge, Polakovicova, Iva, Poon, Ivan K.H., Powell, Bonita H., Prada, Ilaria, Pulliam, Lynn, Quesenberry, Peter, Radeghieri, Annalisa, Raffai, Robert L., Raimondo, Stefania, Rak, Janusz, Ramirez, Marcel I., Raposo, Graça, Rayyan, Morsi S., Regev-Rudzki, Neta, Ricklefs, Fran L., Robbins, Paul D., Roberts, David D., Rodrigues, Silvia C., Rohde, Eva, Rome, Sophie, Rouschop, Kasper M.A., Rughetti, Aurelia, Russell, Ashley E., Saá, Paula, Sahoo, Susmita, Salas-Huenuleo, Edison, Sánchez, Catherine, Saugstad, Julie A., Saul, Meike J., Schiffelers, Raymond M., Schneider, Raphael, Schøyen, Tine Hiorth, Scott, Aaron, Shahaj, Eriomina, Sharma, Shivani, Shatnyeva, Olga, Shekari, Faezeh, Shelke, Ganesh Vilas, Shetty, Ashok K., Shiba, Kiyotaka, Siljander, Pia R-M, Silva, Andreia M., Skowronek, Agata, Snyder II, Orman L., Soares, Rodrigo Pedro, Sódar, Barbara W., Soekmadji, Carolina, Sotillo, Javier, Stahl, Philip D., Stoorvogel, Willem, Stott, Shannon L., Strasser, Erwin F., Swift, Simon, Tahara, Hidetoshi, Tewari, Muneesh, Timms, Kate, Tiwari, Swasti, Tixeira, Rochelle, Tkach, Mercedes, Toh, Wei Seong, Tomasini, Richard, Torrecilhas, Ana Claudia, Tosar, Juan Pablo, Toxavidis, Vasilis, Urbanelli, Lorena, Vader, Pieter, van Balkom, Bas W.M., van der Grein, Susanne G., Van Deun, Jan, van Herwijnen, Martijn J.C., van Keuren-Jensen, Kendall, van Niel, Guillaume, van Royen, Martin E., van Wijnen, Andre J., Vasconcelos, M. Helena, Vechetti Jr., Ivan J., Veit, Tiago D., Vella, Laura J., Velot, Émilie, Verweij, Frederik J., Vestad, Beate, Viñas, Jose L., Visnovitz, Tamás, Vukman, Krisztina V., Wahlgren, Jessica, Watson, Dionysios C., Wauben, Marca H.M., Weaver, Alissa, Webber, Jason P., Weber, Viktoria, Wehman, Ann M., Weiss, Daniel J., Welsh, Joshua A., Wendt, Sebastian, Wheelock, Asa M., Wiener, Zoltán, Witte, Leonie, Wolfram, Joy, Xagorari, Angeliki, Xander, Patricia, Xu, Jing, Yan, Xiaomei, Yáñez-Mó, María, Yin, Hang, Yuana, Yuana, Zappulli, Valentina, Zarubova, Jana, Žėkas, Vytautas, Zhang, Jian-ye, Zhao, Zezhou, Zheng, Lei, Zheutlin, Alexander R., Zickler, Antje M., Zimmermann, Pascale, Zivkovic, Angela M., Zocco, Davide, Zuba-Surma, Ewa K., Théry, Clotilde, Witwer, Kenneth W., Aikawa, Elena, Alcaraz, Maria Jose, Anderson, Johnathon D., Andriantsitohaina, Ramaroson, Antoniou, Anna, Arab, Tanina, Archer, Fabienne, Atkiin-Smith, Georgia K., Ayre, D. Craig, Bach, Jean-Marie, Bachurski, Daniel, Baharvand, Hossein, Balaj, Leonora, Baldacchino, Shawn, Bauer, Natalie N., Baxter, Amy A., Bebawy, Mary, Beckham, Carla, Zavec, Apolonija Bedina, Benmoussa, Abderrahim, Berardi, Anna C., Bergese, Paolo, Bielska, Ewa, Blenkiron, Cherie, Bobis-Wozowicz, Sylwia, Boilard, Eric, Boireau, Wilfrid, Bongiovanni, Antonella, Borràs, Francesc E., Bosch, Steffi, Boulanger, Chantal M., Breakefield, Xandra, Breglio, Andrew M., Brennan, Meadhbh Á, Brigstock, David R., Brisson, Alain, Broekman, Marike L.D., Bromberg, Jacqueline F., Bryl-Górecka, Paulina, Buch, Shilpa, Buck, Amy H., Burger, Dylan, Busatto, Sara, Buschmann, Dominik, Bussolati, Benedetta, Buzás, Edit I., Byrd, James Bryan, Camussi, Giovanni, Carter, David R.F., Caruso, Sarah, Chamley, Lawrence W., Chang, Yu-Ting, Chen, Chichen, Chen, Shuai, Cheng, Lesley, Chin, Andrew R., Clayton, Aled, Clerici, Stefano P., Cocks, Alex, Cocucci, Emanuele, Coffey, Robert J., Cordeiro-da-Silva, Anabela, Couch, Yvonne, Coumans, Frank A.W., Coyle, Beth, Crescitelli, Rossella, Criado, Miria Ferreira, D’ Souza-Schorey, Crislyn, Das, Saumya, Chaudhuri, Amrita Datta, de Candia, Paola, De Santana Junior, Eliezer F., De Wever, Olivier, del Portillo, Hernando A., Demaret, Tanguy, Deville, Sarah, Devitt, Andrew, Dhondt, Bert, Di Vizio, Dolores, Dieterich, Lothar C., Dolo, Vincenza, Dominguez Rubio, Ana Paula, Dominici, Massimo, Dourado, Mauricio R., Driedonks, Tom A.P, Duarte, Filipe V., Duncan, Heather M., Eichenberger, Ramon M., Ekström, Karin, El Andaloussi, Samir, Elie-Caille, Celine, Erdbrügger, Uta, Falcón-Pérez, Juan M., Fatima, Farah, Fish, Jason E., Flores-Bellver, Miguel, Försönits, András, Frelet-Barrand, Annie, Fricke, Fabia, Fuhrmann, Gregor, Gabrielsson, Susanne, Gámez-Valero, Ana, Gardiner, Chris, Gärtner, Kathrin, Gaudin, Raphael, Gho, Yong Song, Giebel, Bernd, Gilbert, Caroline, Gimona, Mario, Giusti, Ilaria, Goberdhan, Deborah C.I., Görgens, André, Gorski, Sharon M., Greening, David W., Gross, Julia Christina, Gualerzi, Alice, Gupta, Gopal N., Gustafson, Dakota, Handberg, Aase, Haraszti, Reka A., Harrison, Paul, Hegyesi, Hargita, Hendrix, An, Hill, Andrew F., Hochberg, Fred H., Hoffmann, Karl F., Holder, Beth, Holthofer, Harry, Hosseinkhani, Baharak, Hu, Guoku, Huang, Yiyao, Huber, Veronica, Hunt, Stuart, Ibrahim, Ahmed Gamal-Eldin, Ikezu, Tsuneya, Inal, Jameel M., Isin, Mustafa, Ivanova, Alena, Jackson, Hannah K., Jacobsen, Soren, Jay, Steven M., Jayachandran, Muthuvel, Jenster, Guido, Jiang, Lanzhou, Johnson, Suzanne M., Jones, Jennifer C., Jong, Ambrose, Jovanovic-Talisman, Tijana, Jung, Stephanie, Kalluri, Raghu, Kano, Shin-ichi, Kaur, Sukhbir, Kawamura, Yumi, Keller, Evan T., Khamari, Delaram, Khomyakova, Elena, Khvorova, Anastasia, Kierulf, Peter, Kim, Kwang Pyo, Kislinger, Thomas, Klingeborn, Mikael, Klinke II, David J., Kornek, Miroslaw, Kosanović, Maja M., Kovács, Árpád Ferenc, Krämer-Albers, Eva-Maria, Krasemann, Susanne, Krause, Mirja, Kurochkin, Igor V., Kusuma, Gina D., Kuypers, Sören, Laitinen, Saara, Langevin, Scott M., Languino, Lucia R., Lannigan, Joanne, Lässer, Cecilia, Laurent, Louise C., Lavieu, Gregory, Lázaro-Ibáñez, Elisa, Lay, Soazig Le, Lee, Myung-Shin, Lee, Yi Xin Fiona, Lemos, Debora S., Lenassi, Metka, Leszczynska, Aleksandra, Li, Isaac T.S., Liao, Ke, Libregts, Sten F., Ligeti, Erzsebet, Lim, Rebecca, Lim, Sai Kiang, Linē, Aija, Linnemannstöns, Karen, Llorente, Alicia, Lombard, Catherine A., Lorenowicz, Magdalena J., Lörincz, Ákos M., Lötvall, Jan, Lovett, Jason, Lowry, Michelle C., Loyer, Xavier, Lu, Quan, Lukomska, Barbara, Lunavat, Taral R., Maas, Sybren L.N., Malhi, Harmeet, Marcilla, Antonio, Mariani, Jacopo, Mariscal, Javier, Martens-Uzunova, Elena S., Martin-Jaular, Lorena, Martinez, M. Carmen, Martins, Vilma Regina, Mathieu, Mathilde, Mathivanan, Suresh, Maugeri, Marco, McGinnis, Lynda K., McVey, Mark J., Meckes, Jr., David G., Meehan, Katie L., Mertens, Inge, Minciacchi, Valentina R., Möller, Andreas, Jørgensen, Malene Møller, Morales-Kastresana, Aizea, Morhayim, Jess, Mullier, François, Muraca, Maurizio, Musante, Luca, Mussack, Veronika, Muth, Dillon C., Myburgh, Kathryn H., Najrana, Tanbir, Nawaz, Muhammad, Nazarenko, Irina, Nejsum, Peter, Neri, Christian, Neri, Tommaso, Nieuwland, Rienk, Nimrichter, Leonardo, Nolan, John P., Nolte-’ t Hoen, Esther N.M., Hooten, Nicole Noren, O’Driscoll, Lorraine, O’Grady, Tina, O’Loghlen, Ana, Ochiya, Takahiro, Olivier, Martin, Ortiz, Alberto, Ortiz, Luis A., Osteikoetxea, Xabier, Ostegaard, Ole, Ostrowski, Matias, Park, Jaesung, Pegtel, D. Michiel, Peinado, Hector, Perut, Francesca, Pfaffl, Michael W., Phinney, Donald G., Pieters, Bartijn C.H., Pink, Ryan C., Pisetsky, David S., von Strandmann, Elke Pogge, Polakovicova, Iva, Poon, Ivan K.H., Powell, Bonita H., Prada, Ilaria, Pulliam, Lynn, Quesenberry, Peter, Radeghieri, Annalisa, Raffai, Robert L., Raimondo, Stefania, Rak, Janusz, Ramirez, Marcel I., Raposo, Graça, Rayyan, Morsi S., Regev-Rudzki, Neta, Ricklefs, Fran L., Robbins, Paul D., Roberts, David D., Rodrigues, Silvia C., Rohde, Eva, Rome, Sophie, Rouschop, Kasper M.A., Rughetti, Aurelia, Russell, Ashley E., Saá, Paula, Sahoo, Susmita, Salas-Huenuleo, Edison, Sánchez, Catherine, Saugstad, Julie A., Saul, Meike J., Schiffelers, Raymond M., Schneider, Raphael, Schøyen, Tine Hiorth, Scott, Aaron, Shahaj, Eriomina, Sharma, Shivani, Shatnyeva, Olga, Shekari, Faezeh, Shelke, Ganesh Vilas, Shetty, Ashok K., Shiba, Kiyotaka, Siljander, Pia R-M, Silva, Andreia M., Skowronek, Agata, Snyder II, Orman L., Soares, Rodrigo Pedro, Sódar, Barbara W., Soekmadji, Carolina, Sotillo, Javier, Stahl, Philip D., Stoorvogel, Willem, Stott, Shannon L., Strasser, Erwin F., Swift, Simon, Tahara, Hidetoshi, Tewari, Muneesh, Timms, Kate, Tiwari, Swasti, Tixeira, Rochelle, Tkach, Mercedes, Toh, Wei Seong, Tomasini, Richard, Torrecilhas, Ana Claudia, Tosar, Juan Pablo, Toxavidis, Vasilis, Urbanelli, Lorena, Vader, Pieter, van Balkom, Bas W.M., van der Grein, Susanne G., Van Deun, Jan, van Herwijnen, Martijn J.C., van Keuren-Jensen, Kendall, van Niel, Guillaume, van Royen, Martin E., van Wijnen, Andre J., Vasconcelos, M. Helena, Vechetti Jr., Ivan J., Veit, Tiago D., Vella, Laura J., Velot, Émilie, Verweij, Frederik J., Vestad, Beate, Viñas, Jose L., Visnovitz, Tamás, Vukman, Krisztina V., Wahlgren, Jessica, Watson, Dionysios C., Wauben, Marca H.M., Weaver, Alissa, Webber, Jason P., Weber, Viktoria, Wehman, Ann M., Weiss, Daniel J., Welsh, Joshua A., Wendt, Sebastian, Wheelock, Asa M., Wiener, Zoltán, Witte, Leonie, Wolfram, Joy, Xagorari, Angeliki, Xander, Patricia, Xu, Jing, Yan, Xiaomei, Yáñez-Mó, María, Yin, Hang, Yuana, Yuana, Zappulli, Valentina, Zarubova, Jana, Žėkas, Vytautas, Zhang, Jian-ye, Zhao, Zezhou, Zheng, Lei, Zheutlin, Alexander R., Zickler, Antje M., Zimmermann, Pascale, Zivkovic, Angela M., Zocco, Davide, and Zuba-Surma, Ewa K.
Abstract: The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points. © 2018, © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.
Published: 2019

55. Selective Regulation of ICAM-1 and RANTES Gene Expression after ICAM-1 Ligation on Human Renal Fibroblasts

Author: Blaber, Robert, Stylianou, Eleni, Clayton, Aled, and Steadman, Robert
Published: 2003

56. Cell surface heparan sulfate proteoglycans control the response of renal interstitial fibroblasts to fibroblast growth factor-2

Author: Clayton, Aled, Thomas, Janet, Thomas, Gareth J., Davies, Malcolm, and Steadman, Robert
Published: 2001

57. Minimal information for studies of extracellular vesicles 2018 (MISEV2018):a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Author: Théry, Clotilde, Witwer, Kenneth W, Aikawa, Elena, Alcaraz, Maria Jose, Anderson, Johnathon D, Andriantsitohaina, Ramaroson, Antoniou, Anna, Arab, Tanina, Archer, Fabienne, Atkin-Smith, Georgia K, Ayre, D Craig, Bach, Jean-Marie, Bachurski, Daniel, Baharvand, Hossein, Balaj, Leonora, Baldacchino, Shawn, Bauer, Natalie N, Baxter, Amy A, Bebawy, Mary, Beckham, Carla, Bedina Zavec, Apolonija, Benmoussa, Abderrahim, Berardi, Anna C, Bergese, Paolo, Bielska, Ewa, Blenkiron, Cherie, Bobis-Wozowicz, Sylwia, Boilard, Eric, Boireau, Wilfrid, Bongiovanni, Antonella, Borràs, Francesc E, Bosch, Steffi, Boulanger, Chantal M, Breakefield, Xandra, Breglio, Andrew M, Brennan, Meadhbh Á, Brigstock, David R, Brisson, Alain, Broekman, Marike Ld, Bromberg, Jacqueline F, Bryl-Górecka, Paulina, Buch, Shilpa, Buck, Amy H, Burger, Dylan, Busatto, Sara, Buschmann, Dominik, Bussolati, Benedetta, Buzás, Edit I, Byrd, James Bryan, Camussi, Giovanni, Carter, David Rf, Caruso, Sarah, Chamley, Lawrence W, Chang, Yu-Ting, Chen, Chihchen, Chen, Shuai, Cheng, Lesley, Chin, Andrew R, Clayton, Aled, Clerici, Stefano P, Cocks, Alex, Cocucci, Emanuele, Coffey, Robert J, Cordeiro-da-Silva, Anabela, Couch, Yvonne, Coumans, Frank Aw, Coyle, Beth, Crescitelli, Rossella, Criado, Miria Ferreira, D'Souza-Schorey, Crislyn, Das, Saumya, Datta Chaudhuri, Amrita, de Candia, Paola, De Santana, Eliezer F, De Wever, Olivier, Del Portillo, Hernando A, Demaret, Tanguy, Deville, Sarah, Devitt, Andrew, Dhondt, Bert, Di Vizio, Dolores, Dieterich, Lothar C, Dolo, Vincenza, Dominguez Rubio, Ana Paula, Dominici, Massimo, Dourado, Mauricio R, Driedonks, Tom Ap, Duarte, Filipe V, Duncan, Heather M, Eichenberger, Ramon M, Ekström, Karin, El Andaloussi, Samir, Elie-Caille, Celine, Erdbrügger, Uta, Falcón-Pérez, Juan M, Fatima, Farah, Fish, Jason E, Flores-Bellver, Miguel, Försönits, András, Frelet-Barrand, Annie, Fricke, Fabia, Fuhrmann, Gregor, Gabrielsson, Susanne, Gámez-Valero, Ana, Gardiner, Chris, Gärtner, Kathrin, Gaudin, Raphael, Gho, Yong Song, Giebel, Bernd, Gilbert, Caroline, Gimona, Mario, Giusti, Ilaria, Goberdhan, Deborah Ci, Görgens, André, Gorski, Sharon M, Greening, David W, Gross, Julia Christina, Gualerzi, Alice, Gupta, Gopal N, Gustafson, Dakota, Handberg, Aase, Haraszti, Reka A, Harrison, Paul, Hegyesi, Hargita, Hendrix, An, Hill, Andrew F, Hochberg, Fred H, Hoffmann, Karl F, Holder, Beth, Holthofer, Harry, Hosseinkhani, Baharak, Hu, Guoku, Huang, Yiyao, Huber, Veronica, Hunt, Stuart, Ibrahim, Ahmed Gamal-Eldin, Ikezu, Tsuneya, Inal, Jameel M, Isin, Mustafa, Ivanova, Alena, Jackson, Hannah K, Jacobsen, Soren, Jay, Steven M, Jayachandran, Muthuvel, Jenster, Guido, Jiang, Lanzhou, Johnson, Suzanne M, Jones, Jennifer C, Jong, Ambrose, Jovanovic-Talisman, Tijana, Jung, Stephanie, Kalluri, Raghu, Kano, Shin-Ichi, Kaur, Sukhbir, Kawamura, Yumi, Keller, Evan T, Khamari, Delaram, Khomyakova, Elena, Khvorova, Anastasia, Kierulf, Peter, Kim, Kwang Pyo, Kislinger, Thomas, Klingeborn, Mikael, Klinke, David J, Kornek, Miroslaw, Kosanović, Maja M, Kovács, Árpád Ferenc, Krämer-Albers, Eva-Maria, Krasemann, Susanne, Krause, Mirja, Kurochkin, Igor V, Kusuma, Gina D, Kuypers, Sören, Laitinen, Saara, Langevin, Scott M, Languino, Lucia R, Lannigan, Joanne, Lässer, Cecilia, Laurent, Louise C, Lavieu, Gregory, Lázaro-Ibáñez, Elisa, Le Lay, Soazig, Lee, Myung-Shin, Lee, Yi Xin Fiona, Lemos, Debora S, Lenassi, Metka, Leszczynska, Aleksandra, Li, Isaac Ts, Liao, Ke, Libregts, Sten F, Ligeti, Erzsebet, Lim, Rebecca, Lim, Sai Kiang, Linē, Aija, Linnemannstöns, Karen, Llorente, Alicia, Lombard, Catherine A, Lorenowicz, Magdalena J, Lörincz, Ákos M, Lötvall, Jan, Lovett, Jason, Lowry, Michelle C, Loyer, Xavier, Lu, Quan, Lukomska, Barbara, Lunavat, Taral R, Maas, Sybren Ln, Malhi, Harmeet, Marcilla, Antonio, Mariani, Jacopo, Mariscal, Javier, Martens-Uzunova, Elena S, Martin-Jaular, Lorena, Martinez, M Carmen, Martins, Vilma Regina, Mathieu, Mathilde, Mathivanan, Suresh, Maugeri, Marco, McGinnis, Lynda K, McVey, Mark J, Meckes, David G, Meehan, Katie L, Mertens, Inge, Minciacchi, Valentina R, Möller, Andreas, Møller Jørgensen, Malene, Morales-Kastresana, Aizea, Morhayim, Jess, Mullier, François, Muraca, Maurizio, Musante, Luca, Mussack, Veronika, Muth, Dillon C, Myburgh, Kathryn H, Najrana, Tanbir, Nawaz, Muhammad, Nazarenko, Irina, Nejsum, Peter, Neri, Christian, Neri, Tommaso, Nieuwland, Rienk, Nimrichter, Leonardo, Nolan, John P, Nolte-'t Hoen, Esther NM, Noren Hooten, Nicole, O'Driscoll, Lorraine, O'Grady, Tina, O'Loghlen, Ana, Ochiya, Takahiro, Olivier, Martin, Ortiz, Alberto, Ortiz, Luis A, Osteikoetxea, Xabier, Østergaard, Ole, Ostrowski, Matias, Park, Jaesung, Pegtel, D Michiel, Peinado, Hector, Perut, Francesca, Pfaffl, Michael W, Phinney, Donald G, Pieters, Bartijn Ch, Pink, Ryan C, Pisetsky, David S, Pogge von Strandmann, Elke, Polakovicova, Iva, Poon, Ivan Kh, Powell, Bonita H, Prada, Ilaria, Pulliam, Lynn, Quesenberry, Peter, Radeghieri, Annalisa, Raffai, Robert L, Raimondo, Stefania, Rak, Janusz, Ramirez, Marcel I, Raposo, Graça, Rayyan, Morsi S, Regev-Rudzki, Neta, Ricklefs, Franz L, Robbins, Paul D, Roberts, David D, Rodrigues, Silvia C, Rohde, Eva, Rome, Sophie, Rouschop, Kasper Ma, Rughetti, Aurelia, Russell, Ashley E, Saá, Paula, Sahoo, Susmita, Salas-Huenuleo, Edison, Sánchez, Catherine, Saugstad, Julie A, Saul, Meike J, Schiffelers, Raymond M, Schneider, Raphael, Schøyen, Tine Hiorth, Scott, Aaron, Shahaj, Eriomina, Sharma, Shivani, Shatnyeva, Olga, Shekari, Faezeh, Shelke, Ganesh Vilas, Shetty, Ashok K, Shiba, Kiyotaka, Siljander, Pia R-M, Silva, Andreia M, Skowronek, Agata, Snyder, Orman L, Soares, Rodrigo Pedro, Sódar, Barbara W, Soekmadji, Carolina, Sotillo, Javier, Stahl, Philip D, Stoorvogel, Willem, Stott, Shannon L, Strasser, Erwin F, Swift, Simon, Tahara, Hidetoshi, Tewari, Muneesh, Timms, Kate, Tiwari, Swasti, Tixeira, Rochelle, Tkach, Mercedes, Toh, Wei Seong, Tomasini, Richard, Torrecilhas, Ana Claudia, Tosar, Juan Pablo, Toxavidis, Vasilis, Urbanelli, Lorena, Vader, Pieter, van Balkom, Bas Wm, van der Grein, Susanne G, Van Deun, Jan, van Herwijnen, Martijn Jc, Van Keuren-Jensen, Kendall, van Niel, Guillaume, van Royen, Martin E, van Wijnen, Andre J, Vasconcelos, M Helena, Vechetti, Ivan J, Veit, Tiago D, Vella, Laura J, Velot, Émilie, Verweij, Frederik J, Vestad, Beate, Viñas, Jose L, Visnovitz, Tamás, Vukman, Krisztina V, Wahlgren, Jessica, Watson, Dionysios C, Wauben, Marca Hm, Weaver, Alissa, Webber, Jason P, Weber, Viktoria, Wehman, Ann M, Weiss, Daniel J, Welsh, Joshua A, Wendt, Sebastian, Wheelock, Asa M, Wiener, Zoltán, Witte, Leonie, Wolfram, Joy, Xagorari, Angeliki, Xander, Patricia, Xu, Jing, Yan, Xiaomei, Yáñez-Mó, María, Yin, Hang, Yuana, Yuana, Zappulli, Valentina, Zarubova, Jana, Žėkas, Vytautas, Zhang, Jian-Ye, Zhao, Zezhou, Zheng, Lei, Zheutlin, Alexander R, Zickler, Antje M, Zimmermann, Pascale, Zivkovic, Angela M, Zocco, Davide, Zuba-Surma, Ewa K, dB&C I&I, LS Celbiologie-Algemeen, Celbiologie, Afd Pharmaceutics, Sub General Pharmaceutics, Sub Biomol.Mass Spect. and Proteomics, Afd Pharmacology, Urology, Pathology, Medical Oncology, Immunité et cancer, Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Johns Hopkins University School of Medicine [Baltimore], Stress Oxydant et Pathologies Métaboliques (SOPAM), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 (PRISM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Infections Virales et Pathologie Comparée - UMR 754 (IVPC), Institut National de la Recherche Agronomique (INRA)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Immuno-Endocrinologie Cellulaire et Moléculaire [Nantes] (IECM), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Department for Molecular Biology and Nanobiotechnology, National Institute of chemitry, Slovenia, Biologie, génétique et thérapies ostéoarticulaires et respiratoires (BIOTARGEN), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes, Paris-Centre de Recherche Cardiovasculaire (PARCC - UMR-S U970), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Physiopathologie des Adaptations Nutritionnelles (PhAN), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie (IECB), Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS), Molecular Biotechnology Center, Università degli studi di Torino = University of Turin (UNITO), Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University (JCU), Department of Oncology - Pathology, Cancer Center Karolinska [Karolinska Institutet] (CCK), Karolinska Institutet [Stockholm]-Karolinska Institutet [Stockholm], Departamento de Ciências Biológicas, Universidade do Porto = University of Porto, Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Cancer Research Institute Ghent (CRIG), Universiteit Gent = Ghent University [Belgium] (UGENT), Department of Medical and Surgical Sciences for Children and Adults [Modena, Italy] (Laboratory of Cellular Therapy), Università degli Studi di Modena e Reggio Emilia (UNIMORE), Clinical Research Center, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, Huddinge, Sweden, Karolinska Institutet [Stockholm]-Karolinska University Hospital [Stockholm], Center for Cooperative Research in Biosciences (CIC bioGUNE), Partner site Munich, German Centre for Infection Research (DZIF), Institute for Transfusion Medicine, University Hospital Essen, Universität Duisburg-Essen [Essen], Mécanismes Adaptatifs et Evolution (MECADEV), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Psychiatry, Institute of Biological, Environmental and Rural Sciences (IBERS), Aberystwyth University, Department of Bacteriology and Immunology [Helsinki], Haartman Institute [Helsinki], Faculty of Medecine [Helsinki], Helsingin yliopisto = Helsingfors universitet = University of Helsinki-Helsingin yliopisto = Helsingfors universitet = University of Helsinki-Faculty of Medecine [Helsinki], Helsingin yliopisto = Helsingfors universitet = University of Helsinki-Helsingin yliopisto = Helsingfors universitet = University of Helsinki, Rigshospitalet [Copenhagen], Copenhagen University Hospital, Dalhousie University [Halifax], Department of Biology, Molecular Cell Biology, University of Mainz, Johannes Gutenberg - Universität Mainz = Johannes Gutenberg University (JGU), Glycobiologie et signalisation cellulaire, Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM), Krefting Research Centre, Department of Internal Medicine and Clinical Nutrition, University of Gothenburg (GU), Universidad de Alicante, École supérieure du professorat et de l'éducation - Académie de Créteil (UPEC ESPE Créteil), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), University of Antwerp (UA), Université Catholique de Louvain = Catholic University of Louvain (UCL), Research Institute, IRCCS Ospedale Pediatrico Bambino Gesù [Roma], Department of Veterinary Disease Biology [Copenhagen], Faculty of Health and Medical Sciences, University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU), Biologie et Pathologie du Neurone (Brain-C), Adaptation Biologique et Vieillissement = Biological Adaptation and Ageing (B2A), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Department of Mathematics and Statistics, American University, University of Pretoria [South Africa], Ecole des Ingénieurs de la Ville de Paris (EIVP), Universitat Pompeu Fabra [Barcelona] (UPF), Instituto de Investigaciones Biomedicas, Universidad Nacional Autónoma de México (UNAM), Istituto Ortopedico Rizzoli, Department of Molecular Therapeutics, The Scripps Research Institute, Laboratoire d'Informatique de Grenoble (LIG ), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Montreal Children's Hospital, McGill University Health Center [Montreal] (MUHC), Compartimentation et dynamique cellulaires (CDC), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie [Paris]-Centre National de la Recherche Scientifique (CNRS), National Cancer Institute [Bethesda] (NCI-NIH), National Institutes of Health [Bethesda] (NIH), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Cardiovascular Research Center, Massachusetts General Hospital [Boston], University Medical Center [Utrecht], University of Toronto, Fiocruz Minas - René Rachou Research Center / Instituto René Rachou [Belo Horizonte, Brésil], Fundação Oswaldo Cruz (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Federal University of Sao Paulo (Unifesp), Functional Genomics / Genómica Funcional [Montevideo], Institut Pasteur de Montevideo, Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università degli Studi di Perugia (UNIPG), Hospital Santa Cristina Instituto de Investigación Sanitaria Princesa C, Unidad de Investigación, Institut Curie [Paris]-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Department of Physiology, University of California [San Francisco] (UCSF), University of California-University of California, University of Vermont [Burlington], Peking University [Beijing], Shandong Agricultural University (SDAU), State Key Laboratory of Quality Research in Chinese Medicine Taipa, Macau SAR, (Institute of Chinese Medical Sciences), Human Genetics, Universität Ulm - Ulm University [Ulm, Allemagne], INSERM, Institut Curie, INCa [INCA-11548], French National Research Agency [ANR-10-IDEX-0001-02 PSL*, ANR-11-LABX-0043], SIDACTION [17-1-AAE-1138], Fondation ARC [PGA1 RF20180206962, PJA 20171206453], NIDA [DA040385, DA047807], Ministry of Education, NIA [AG057430], NIMH [MH118164], Institut National de la Recherche Agronomique (INRA)-École Pratique des Hautes Études (EPHE), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN), Université Sciences et Technologies - Bordeaux 1 (UB)-Centre National de la Recherche Scientifique (CNRS), Universiteit Gent = Ghent University (UGENT), Università degli Studi di Modena e Reggio Emilia = University of Modena and Reggio Emilia (UNIMORE), Universität Duisburg-Essen = University of Duisburg-Essen [Essen], Biotechnology and Biological Sciences Research Council (BBSRC)-Aberystwyth University, University of Copenhagen = Københavns Universitet (UCPH)-University of Copenhagen = Københavns Universitet (UCPH), Universidad Nacional Autónoma de México = National Autonomous University of Mexico (UNAM), The Scripps Research Institute [La Jolla, San Diego], Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ), Università degli Studi di Perugia = University of Perugia (UNIPG), Instituto de Investigacion Sanitaria del Hospital de la Princesa, Hospital Universitario de La Princesa, University of California [San Francisco] (UC San Francisco), University of California (UC)-University of California (UC), ANR-17-CE09-0025,MADNESS,Une approche microfluidique générique pour la qualification des nanoparticules biologiques(2017), Institut National de la Recherche Agronomique (INRA)-École pratique des hautes études (EPHE)-Université Claude Bernard Lyon 1 (UCBL), Biomedical Engineering and Physics, ACS - Atherosclerosis & ischemic syndromes, ACS - Microcirculation, Laboratory Specialized Diagnostics & Research, Radiotherapie, RS: GROW - R2 - Basic and Translational Cancer Biology, Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Université Nice Sophia Antipolis (... - 2019) (UNS), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Vétérinaire de Nantes-Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA), Università degli studi di Torino (UNITO), Universidade do Porto, University of Helsinki-University of Helsinki-Faculty of Medecine [Helsinki], University of Helsinki-University of Helsinki, Johannes Gutenberg - Universität Mainz (JGU), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Université Pierre et Marie Curie - Paris 6 (UPMC), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Université de Toronto [Canada], Institut Curie-Institut National de la Santé et de la Recherche Médicale (INSERM), Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192 (PRISM), Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Université de Franche-Comté (UFC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Technologie de Belfort-Montbeliard (UTBM), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Physiopathologie de la Résorption Osseuse et Thérapie des Tumeurs Osseuses Primitives, Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM), Universidade do Porto [Porto], Ghent University [Belgium] (UGENT), FEMTO-ST Institute, Université de Technologie de Belfort-Montbeliard (UTBM)-Université de Franche-Comté (UFC)-CNRS : UMR6174, Mécanismes adaptatifs : des organismes aux communautés (MECADEV), Centre National de la Recherche Scientifique (CNRS)-Muséum national d'Histoire naturelle (MNHN), Johannes Gutenberg - University of Mainz (JGU), Université Catholique de Louvain (UCL), Universitat Pompeu Fabra [Barcelona], Laboratoire d'Informatique de Grenoble (LIG), Université Pierre Mendès France - Grenoble 2 (UPMF)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-Institut National Polytechnique de Grenoble (INPG)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Centre National de la Recherche Scientifique (CNRS)-Institut Curie-Université Pierre et Marie Curie - Paris 6 (UPMC), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Laboratoire Réactions et Génie des Procédés (LRGP), Fiocruz Minas - René Rachou Research Center / Instituto René Rachou, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Aix Marseille Université (AMU), Functional Genomics Unit, Institut Curie-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), University of Vermont College of Medicine [Burlington, VT, USA], Extracellular Vesicles, Molecular and Integrative Biosciences Research Programme, Thery, C., Witwer, K. W., Aikawa, E., Alcaraz, M. J., Anderson, J. D., Andriantsitohaina, R., Antoniou, A., Arab, T., Archer, F., Atkin-Smith, G. K., Ayre, D. C., Bach, J. -M., Bachurski, D., Baharvand, H., Balaj, L., Baldacchino, S., Bauer, N. N., Baxter, A. A., Bebawy, M., Beckham, C., Bedina Zavec, A., Benmoussa, A., Berardi, A. C., Bergese, P., Bielska, E., Blenkiron, C., Bobis-Wozowicz, S., Boilard, E., Boireau, W., Bongiovanni, A., Borras, F. E., Bosch, S., Boulanger, C. M., Breakefield, X., Breglio, A. M., Brennan, M. A., Brigstock, D. R., Brisson, A., Broekman, M. L. D., Bromberg, J. F., Bryl-Gorecka, P., Buch, S., Buck, A. H., Burger, D., Busatto, S., Buschmann, D., Bussolati, B., Buzas, E. I., Byrd, J. B., Camussi, G., Carter, D. R. F., Caruso, S., Chamley, L. W., Chang, Y. -T., Chaudhuri, A. D., Chen, C., Chen, S., Cheng, L., Chin, A. R., Clayton, A., Clerici, S. P., Cocks, A., Cocucci, E., Coffey, R. J., Cordeiro-da-Silva, A., Couch, Y., Coumans, F. A. W., Coyle, B., Crescitelli, R., Criado, M. F., D'Souza-Schorey, C., Das, S., de Candia, P., De Santana, E. F., De Wever, O., del Portillo, H. A., Demaret, T., Deville, S., Devitt, A., Dhondt, B., Di Vizio, D., Dieterich, L. C., Dolo, V., Dominguez Rubio, A. P., Dominici, M., Dourado, M. R., Driedonks, T. A. P., Duarte, F. V., Duncan, H. M., Eichenberger, R. M., Ekstrom, K., EL Andaloussi, S., Elie-Caille, C., Erdbrugger, U., Falcon-Perez, J. M., Fatima, F., Fish, J. E., Flores-Bellver, M., Forsonits, A., Frelet-Barrand, A., Fricke, F., Fuhrmann, G., Gabrielsson, S., Gamez-Valero, A., Gardiner, C., Gartner, K., Gaudin, R., Gho, Y. S., Giebel, B., Gilbert, C., Gimona, M., Giusti, I., Goberdhan, D. C. I., Gorgens, A., Gorski, S. M., Greening, D. W., Gross, J. C., Gualerzi, A., Gupta, G. N., Gustafson, D., Handberg, A., Haraszti, R. A., Harrison, P., Hegyesi, H., Hendrix, A., Hill, A. F., Hochberg, F. H., Hoffmann, K. F., Holder, B., Holthofer, H., Hosseinkhani, B., Hu, G., Huang, Y., Huber, V., Hunt, S., Ibrahim, A. G. -E., Ikezu, T., Inal, J. M., Isin, M., Ivanova, A., Jackson, H. K., Jacobsen, S., Jay, S. M., Jayachandran, M., Jenster, G., Jiang, L., Johnson, S. M., Jones, J. C., Jong, A., Jovanovic-Talisman, T., Jung, S., Kalluri, R., Kano, S. -I., Kaur, S., Kawamura, Y., Keller, E. T., Khamari, D., Khomyakova, E., Khvorova, A., Kierulf, P., Kim, K. P., Kislinger, T., Klingeborn, M., Klinke, D. J., Kornek, M., Kosanovic, M. M., Kovacs, A. F., Kramer-Albers, E. -M., Krasemann, S., Krause, M., Kurochkin, I. V., Kusuma, G. D., Kuypers, S., Laitinen, S., Langevin, S. M., Languino, L. R., Lannigan, J., Lasser, C., Laurent, L. C., Lavieu, G., Lazaro-Ibanez, E., Le Lay, S., Lee, M. -S., Lee, Y. X. F., Lemos, D. S., Lenassi, M., Leszczynska, A., Li, I. T. S., Liao, K., Libregts, S. F., Ligeti, E., Lim, R., Lim, S. K., Line, A., Linnemannstons, K., Llorente, A., Lombard, C. A., Lorenowicz, M. J., Lorincz, A. M., Lotvall, J., Lovett, J., Lowry, M. C., Loyer, X., Lu, Q., Lukomska, B., Lunavat, T. R., Maas, S. L. N., Malhi, H., Marcilla, A., Mariani, J., Mariscal, J., Martens-Uzunova, E. S., Martin-Jaular, L., Martinez, M. C., Martins, V. R., Mathieu, M., Mathivanan, S., Maugeri, M., Mcginnis, L. K., Mcvey, M. J., Meckes, D. G., Meehan, K. L., Mertens, I., Minciacchi, V. R., Moller, A., Moller Jorgensen, M., Morales-Kastresana, A., Morhayim, J., Mullier, F., Muraca, M., Musante, L., Mussack, V., Muth, D. C., Myburgh, K. H., Najrana, T., Nawaz, M., Nazarenko, I., Nejsum, P., Neri, C., Neri, T., Nieuwland, R., Nimrichter, L., Nolan, J. P., Nolte-'t Hoen, E. N. M., Noren Hooten, N., O'Driscoll, L., O'Grady, T., O'Loghlen, A., Ochiya, T., Olivier, M., Ortiz, A., Ortiz, L. A., Osteikoetxea, X., Ostegaard, O., Ostrowski, M., Park, J., Pegtel, D. M., Peinado, H., Perut, F., Pfaffl, M. W., Phinney, D. G., Pieters, B. C. H., Pink, R. C., Pisetsky, D. S., Pogge von Strandmann, E., Polakovicova, I., Poon, I. K. H., Powell, B. H., Prada, I., Pulliam, L., Quesenberry, P., Radeghieri, A., Raffai, R. L., Raimondo, S., Rak, J., Ramirez, M. I., Raposo, G., Rayyan, M. S., Regev-Rudzki, N., Ricklefs, F. L., Robbins, P. D., Roberts, D. D., Rodrigues, S. C., Rohde, E., Rome, S., Rouschop, K. M. A., Rughetti, A., Russell, A. E., Saa, P., Sahoo, S., Salas-Huenuleo, E., Sanchez, C., Saugstad, J. A., Saul, M. J., Schiffelers, R. M., Schneider, R., Schoyen, T. H., Scott, A., Shahaj, E., Sharma, S., Shatnyeva, O., Shekari, F., Shelke, G. V., Shetty, A. K., Shiba, K., Siljander, P. R. -M., Silva, A. M., Skowronek, A., Snyder, O. L., Soares, R. P., Sodar, B. W., Soekmadji, C., Sotillo, J., Stahl, P. D., Stoorvogel, W., Stott, S. L., Strasser, E. F., Swift, S., Tahara, H., Tewari, M., Timms, K., Tiwari, S., Tixeira, R., Tkach, M., Toh, W. S., Tomasini, R., Torrecilhas, A. C., Tosar, J. P., Toxavidis, V., Urbanelli, L., Vader, P., van Balkom, B. W. M., van der Grein, S. G., Van Deun, J., van Herwijnen, M. J. C., Van Keuren-Jensen, K., van Niel, G., van Royen, M. E., van Wijnen, A. J., Vasconcelos, M. H., Vechetti, I. J., Veit, T. D., Vella, L. J., Velot, E., Verweij, F. J., Vestad, B., Vinas, J. L., Visnovitz, T., Vukman, K. V., Wahlgren, J., Watson, D. C., Wauben, M. H. M., Weaver, A., Webber, J. P., Weber, V., Wehman, A. M., Weiss, D. J., Welsh, J. A., Wendt, S., Wheelock, A. M., Wiener, Z., Witte, L., Wolfram, J., Xagorari, A., Xander, P., Xu, J., Yan, X., Yanez-Mo, M., Yin, H., Yuana, Y., Zappulli, V., Zarubova, J., Zekas, V., Zhang, J. -Y., Zhao, Z., Zheng, L., Zheutlin, A. R., Zickler, A. M., Zimmermann, P., Zivkovic, A. M., Zocco, D., Zuba-Surma, E. K., dB&C I&I, LS Celbiologie-Algemeen, Celbiologie, Afd Pharmaceutics, Sub General Pharmaceutics, Sub Biomol.Mass Spect. and Proteomics, Afd Pharmacology, CCA - Imaging and biomarkers, Amsterdam Neuroscience - Neuroinfection & -inflammation, and Amsterdam Neuroscience - Cellular & Molecular Mechanisms
Subjects: ectosome, ectosomes, exosomes, extracellular vesicles, guidelines, microparticles, microvesicles, minimal information requirements, reproducibility, rigor, standardization, Histology, Cell Biology, [SDV]Life Sciences [q-bio], size-exclusion, Medicine and Health Sciences, CELL-DERIVED MICROPARTICLES, FIELD-FLOW FRACTIONATION, requirements, circulating, ComputingMilieux_MISCELLANEOUS, Manchester Cancer Research Centre, lcsh:Cytology, PROSTATE-CANCER, microparticle, Cell interaction, microvesicle, chromatography, Position Paper, guideline, Life Sciences & Biomedicine, ectosomes, exosomes, extracellular vesicles, guidelines, microparticles, microvesicles, minimal information requirements, reproducibility, rigor, standardization, MEMBRANE-VESICLES, FETAL BOVINE, Ectosomes, Exosomes, Extracellular Vesicles, Guidelines, Microparticles, Microvesicles, Minimal Information Requirements, Reproducibility, Rigor, Standardization, CIRCULATING MICROPARTICLES, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, ddc:570, exosome, SURFACE-PLASMON RESONANCE, ddc:610, lcsh:QH573-671, Biology, Interacció cel·lular, Science & Technology, ResearchInstitutes_Networks_Beacons/mcrc, Cell membranes, HUMAN URINARY EXOSOMES, PREANALYTICAL PARAMETERS, minimal information requirement, SIZE-EXCLUSION CHROMATOGRAPHY, 1182 Biochemistry, cell and molecular biology, extracellular vesicle, Human medicine, Membranes cel·lulars
Abstract: The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
Published: 2018

58. Enzymatically oxidized phospholipids restore thrombin generation in coagulation factor deficiencies

Author: Slatter, David A., Percy, Charles L., Allen-Redpath, Keith, Gajsiewicz, Joshua M., Brooks, Nick J., Clayton, Aled, Tyrrell, Victoria J., Rosas, Marcela, Lauder, Sarah N., Watson, Andrew, Dul, Maria, Garcia-Diaz, Yoel, Aldrovandi, Maceler, Heurich, Meike, Hall, Judith, Morrissey, James H., Lacroix-Desmazes, Sebastien, Delignat, Sandrine, Jenkins, P. Vincent, Collins, Peter W., O'Donnell, Valerie B., Department of Oncology - Pathology, Cancer Center Karolinska [Karolinska Institutet] (CCK), Karolinska Institutet [Stockholm]-Karolinska Institutet [Stockholm], NASA Ames Research Center (ARC), Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Cardiac Medicine, National Heart and Lung Institute, Imperial College London, École pratique des hautes études (EPHE), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)
Subjects: Adult, Blood Platelets, Male, Lipoproteins, Hemorrhage, Factor VIIa, Hemophilia A, Thromboplastin, Factor IX, Mice, Hydroxyeicosatetraenoic Acids, Animals, Humans, Blood Coagulation, Phospholipids, Aged, Aged, 80 and over, Hemostasis, Cardiopulmonary Bypass, Factor VIII, Thrombin, Middle Aged, Surface Plasmon Resonance, Blood Coagulation Factors, Neoplasm Proteins, Mice, Inbred C57BL, Cysteine Endopeptidases, Factor X, [SDV.IMM]Life Sciences [q-bio]/Immunology, lipids (amino acids, peptides, and proteins), Carrier Proteins, Research Article, circulatory and respiratory physiology
Abstract: International audience; Hemostatic defects are treated using coagulation factors; however, clot formation also requires a procoagulant phospholipid (PL) surface. Here, we show that innate immune cell-derived enzymatically oxidized phospholipids (eoxPL) termed hydroxyeicosatetraenoic acid-phospholipids (HETE-PLs) restore hemostasis in human and murine conditions of pathological bleeding. HETE-PLs abolished blood loss in murine hemophilia A and enhanced coagulation in factor VIII-(FVIII-), FIX-, and FX-deficient human plasma. HETE-PLs were decreased in platelets from patients after cardiopulmonary bypass (CPB). To explore molecular mechanisms, the ability of eoxPL to stimulate individual isolated coagulation factor/cofactor complexes was tested in vitro. Extrinsic tenase (FVIIa/tissue factor [TF]), intrinsic tenase (FVIIIa/FIXa), and prothrombinase (FVa/FXa) all were enhanced by both HETE-PEs and HETE-PCs, suggesting a common mechanism involving the fatty acid moiety. In plasma, 9-, 15-, and 12-HETE-PLs were more effective than 5-, 11-, or 8-HETE-PLs, indicating positional isomer specificity. Coagulation was enhanced at lower lipid/factor ratios, consistent with a more concentrated area for protein binding. Surface plasmon resonance confirmed binding of FII and FX to HETE-PEs. HETE-PEs increased membrane curvature and thickness, but not surface charge or homogeneity, possibly suggesting increased accessibility to cations/factors. In summary, innate immune-derived eoxPL enhance calcium-dependent coagulation factor function, and their potential utility in bleeding disorders is proposed.
Published: 2018

59. Considerations towards a roadmap for collection, handling and storage of blood extracellular vesicles

Author: Clayton, Aled, primary, Boilard, Eric, additional, Buzas, Edit I, additional, Cheng, Lesley, additional, Falcón-Perez, Juan Manual, additional, Gardiner, Chris, additional, Gustafson, Dakota, additional, Gualerzi, Alice, additional, Hendrix, An, additional, Hoffman, Andrew, additional, Jones, Jennifer, additional, Lässer, Cecilia, additional, Lawson, Charlotte, additional, Lenassi, Metka, additional, Nazarenko, Irina, additional, O’Driscoll, Lorraine, additional, Pink, Ryan, additional, Siljander, Pia R-M, additional, Soekmadji, Carolina, additional, Wauben, Marca, additional, Welsh, Joshua A, additional, Witwer, Ken, additional, Zheng, Lei, additional, and Nieuwland, Rienk, additional
Published: 2019
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60. Exosomes populate the cerebrospinal fluid of preterm infants with post‐haemorrhagic hydrocephalus

Author: Spaull, Robert, primary, McPherson, Bryony, additional, Gialeli, Andriana, additional, Clayton, Aled, additional, Uney, James, additional, Heep, Axel, additional, and Cordero‐Llana, Óscar, additional
Published: 2019
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61. Can urinary exosomes act as treatment response markers in prostate cancer?

Author: Tabi Zsuzsanna, Mason Malcolm D, Court Jacquelyn, Staffurth John, Welton Joanne, Mitchell Paul J, and Clayton Aled
Subjects: Medicine
Abstract: Abstract Background Recently, nanometer sized vesicles (termed exosomes) have been described as a component of urine. Such vesicles may be a useful non-invasive source of markers in renal disease. Their utility as a source of markers in urological cancer remains unstudied. Our aim in this study was to investigate the feasibility and value of analysing urinary exosomes in prostate cancer patients undergoing standard therapy. Methods Ten patients (with locally advanced PCa) provided spot urine specimens at three time points during standard therapy. Patients received 3–6 months neoadjuvant androgen deprivation therapy prior to radical radiotherapy, comprising a single phase delivering 55 Gy in 20 fractions to the prostate and 44 Gy in 20 fractions to the pelvic nodes. Patients were continued on adjuvant ADT according to clinical need. Exosomes were purified, and the phenotype compared to exosomes isolated from the prostate cancer cell line LNcaP. A control group of 10 healthy donors was included. Serum PSA was used as a surrogate treatment response marker. Exosomes present in urine were quantified, and expression of prostate markers (PSA and PSMA) and tumour-associated marker 5T4 was examined. Results The quantity and quality of exosomes present in urine was highly variable, even though we handled all materials freshly and used methods optimized for obtaining highly pure exosomes. There was approx 2-fold decrease in urinary exosome content following 12 weeks ADT, but this was not sustained during radiotherapy. Nevertheless, PSA and PSMA were present in 20 of 24 PCa specimens, and not detected in healthy donor specimens. There was a clear treatment-related decrease in exosomal prostate markers in 1 (of 8) patient. Conclusion Evaluating urinary-exosomes remains difficult, given the variability of exosomes in urine specimens. Nevertheless, this approach holds promise as a non-invasive source of multiple markers of malignancy that could provide clinically useful information.
Published: 2009
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62. Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017

Author: Clayton, Aled, Buschmann, Dominik, Byrd, J. Brian, Carter, David R. F., Cheng, Lesley, Compton, Carolyn, Daaboul, George, Devitt, Andrew, Falcon-Perez, Juan Manuel, Gardiner, Chris, Gustafson, Dakota, Harrison, Paul, Helmbrecht, Clemens, Hendrix, An, Hill, Andrew, Hoffman, Andrew, Jones, Jennifer C., Kalluri, Raghu, Kang, Ji Yoon, Kirchner, Benedikt, Lässer, Cecilia, Lawson, Charlotte, Lenassi, Metka, Levin, Carina, Llorente, Alicia, Martens-Uzunova, Elena S., Möller, Andreas, Musante, Luca, Ochiya, Takahiro, Pink, Ryan C., Tahara, Hidetoshi, Wauben, Marca H. M., Webber, Jason P., Welsh, Joshua A., Witwer, Kenneth W., Yin, Hang, Nieuwland, Rienk, Clayton, Aled, Buschmann, Dominik, Byrd, J. Brian, Carter, David R. F., Cheng, Lesley, Compton, Carolyn, Daaboul, George, Devitt, Andrew, Falcon-Perez, Juan Manuel, Gardiner, Chris, Gustafson, Dakota, Harrison, Paul, Helmbrecht, Clemens, Hendrix, An, Hill, Andrew, Hoffman, Andrew, Jones, Jennifer C., Kalluri, Raghu, Kang, Ji Yoon, Kirchner, Benedikt, Lässer, Cecilia, Lawson, Charlotte, Lenassi, Metka, Levin, Carina, Llorente, Alicia, Martens-Uzunova, Elena S., Möller, Andreas, Musante, Luca, Ochiya, Takahiro, Pink, Ryan C., Tahara, Hidetoshi, Wauben, Marca H. M., Webber, Jason P., Welsh, Joshua A., Witwer, Kenneth W., Yin, Hang, and Nieuwland, Rienk
Abstract: This report summarises the presentations and activities of the ISEV Workshop on extracellular vesicle biomarkers held in Birmingham, UK during December 2017. Among the key messages was broad agreement about the importance of biospecimen science. Much greater attention needs to be paid towards the provenance of collected samples. The workshop also highlighted clear gaps in our knowledge about pre-analytical factors that alter extracellular vesicles (EVs). The future utility of certified standards for credentialing of instruments and software, to analyse EV and for tracking the influence of isolation steps on the structure and content of EVs were also discussed. Several example studies were presented, demonstrating the potential utility for EVs in disease diagnosis, prognosis, longitudinal serial testing and stratification of patients. The conclusion of the workshop was that more effort focused on pre-analytical issues and benchmarking of isolation methods is needed to strengthen collaborations and advance more effective biomarkers.
Published: 2018

63. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Author: dB&C I&I, LS Celbiologie-Algemeen, Celbiologie, Afd Pharmaceutics, Sub General Pharmaceutics, Sub Biomol.Mass Spect. and Proteomics, Afd Pharmacology, Théry, Clotilde, Witwer, Kenneth W, Aikawa, Elena, Alcaraz, Maria Jose, Anderson, Johnathon D, Andriantsitohaina, Ramaroson, Antoniou, Anna, Arab, Tanina, Archer, Fabienne, Atkin-Smith, Georgia K, Ayre, D Craig, Bach, Jean-Marie, Bachurski, Daniel, Baharvand, Hossein, Balaj, Leonora, Baldacchino, Shawn, Bauer, Natalie N, Baxter, Amy A, Bebawy, Mary, Beckham, Carla, Bedina Zavec, Apolonija, Benmoussa, Abderrahim, Berardi, Anna C, Bergese, Paolo, Bielska, Ewa, Blenkiron, Cherie, Bobis-Wozowicz, Sylwia, Boilard, Eric, Boireau, Wilfrid, Bongiovanni, Antonella, Borràs, Francesc E, Bosch, Steffi, Boulanger, Chantal M, Breakefield, Xandra, Breglio, Andrew M, Brennan, Meadhbh Á, Brigstock, David R, Brisson, Alain, Broekman, Marike Ld, Bromberg, Jacqueline F, Bryl-Górecka, Paulina, Buch, Shilpa, Buck, Amy H, Burger, Dylan, Busatto, Sara, Buschmann, Dominik, Bussolati, Benedetta, Buzás, Edit I, Byrd, James Bryan, Camussi, Giovanni, Carter, David Rf, Caruso, Sarah, Chamley, Lawrence W, Chang, Yu-Ting, Chen, Chihchen, Chen, Shuai, Cheng, Lesley, Chin, Andrew R, Clayton, Aled, Clerici, Stefano P, Cocks, Alex, Cocucci, Emanuele, Coffey, Robert J, Cordeiro-da-Silva, Anabela, Couch, Yvonne, Coumans, Frank Aw, Coyle, Beth, Crescitelli, Rossella, Criado, Miria Ferreira, D'Souza-Schorey, Crislyn, Das, Saumya, Datta Chaudhuri, Amrita, de Candia, Paola, De Santana, Eliezer F, De Wever, Olivier, Del Portillo, Hernando A, Demaret, Tanguy, Deville, Sarah, Devitt, Andrew, Dhondt, Bert, Di Vizio, Dolores, Dieterich, Lothar C, Dolo, Vincenza, Dominguez Rubio, Ana Paula, Dominici, Massimo, Dourado, Mauricio R, Driedonks, Tom Ap, Duarte, Filipe V, Duncan, Heather M, Eichenberger, Ramon M, Ekström, Karin, El Andaloussi, Samir, Elie-Caille, Celine, Erdbrügger, Uta, Falcón-Pérez, Juan M, Fatima, Farah, Fish, Jason E, Flores-Bellver, Miguel, Försönits, András, Frelet-Barrand, Annie, Fricke, Fabia, Fuhrmann, Gregor, Gabrielsson, Susanne, Gámez-Valero, Ana, Gardiner, Chris, Gärtner, Kathrin, Gaudin, Raphael, Gho, Yong Song, Giebel, Bernd, Gilbert, Caroline, Gimona, Mario, Giusti, Ilaria, Goberdhan, Deborah Ci, Görgens, André, Gorski, Sharon M, Greening, David W, Gross, Julia Christina, Gualerzi, Alice, Gupta, Gopal N, Gustafson, Dakota, Handberg, Aase, Haraszti, Reka A, Harrison, Paul, Hegyesi, Hargita, Hendrix, An, Hill, Andrew F, Hochberg, Fred H, Hoffmann, Karl F, Holder, Beth, Holthofer, Harry, Hosseinkhani, Baharak, Hu, Guoku, Huang, Yiyao, Huber, Veronica, Hunt, Stuart, Ibrahim, Ahmed Gamal-Eldin, Ikezu, Tsuneya, Inal, Jameel M, Isin, Mustafa, Ivanova, Alena, Jackson, Hannah K, Jacobsen, Soren, Jay, Steven M, Jayachandran, Muthuvel, Jenster, Guido, Jiang, Lanzhou, Johnson, Suzanne M, Jones, Jennifer C, Jong, Ambrose, Jovanovic-Talisman, Tijana, Jung, Stephanie, Kalluri, Raghu, Kano, Shin-Ichi, Kaur, Sukhbir, Kawamura, Yumi, Keller, Evan T, Khamari, Delaram, Khomyakova, Elena, Khvorova, Anastasia, Kierulf, Peter, Kim, Kwang Pyo, Kislinger, Thomas, Klingeborn, Mikael, Klinke, David J, Kornek, Miroslaw, Kosanović, Maja M, Kovács, Árpád Ferenc, Krämer-Albers, Eva-Maria, Krasemann, Susanne, Krause, Mirja, Kurochkin, Igor V, Kusuma, Gina D, Kuypers, Sören, Laitinen, Saara, Langevin, Scott M, Languino, Lucia R, Lannigan, Joanne, Lässer, Cecilia, Laurent, Louise C, Lavieu, Gregory, Lázaro-Ibáñez, Elisa, Le Lay, Soazig, Lee, Myung-Shin, Lee, Yi Xin Fiona, Lemos, Debora S, Lenassi, Metka, Leszczynska, Aleksandra, Li, Isaac Ts, Liao, Ke, Libregts, Sten F, Ligeti, Erzsebet, Lim, Rebecca, Lim, Sai Kiang, Linē, Aija, Linnemannstöns, Karen, Llorente, Alicia, Lombard, Catherine A, Lorenowicz, Magdalena J, Lörincz, Ákos M, Lötvall, Jan, Lovett, Jason, Lowry, Michelle C, Loyer, Xavier, Lu, Quan, Lukomska, Barbara, Lunavat, Taral R, Maas, Sybren Ln, Malhi, Harmeet, Marcilla, Antonio, Mariani, Jacopo, Mariscal, Javier, Martens-Uzunova, Elena S, Martin-Jaular, Lorena, Martinez, M Carmen, Martins, Vilma Regina, Mathieu, Mathilde, Mathivanan, Suresh, Maugeri, Marco, McGinnis, Lynda K, McVey, Mark J, Meckes, David G, Meehan, Katie L, Mertens, Inge, Minciacchi, Valentina R, Möller, Andreas, Møller Jørgensen, Malene, Morales-Kastresana, Aizea, Morhayim, Jess, Mullier, François, Muraca, Maurizio, Musante, Luca, Mussack, Veronika, Muth, Dillon C, Myburgh, Kathryn H, Najrana, Tanbir, Nawaz, Muhammad, Nazarenko, Irina, Nejsum, Peter, Neri, Christian, Neri, Tommaso, Nieuwland, Rienk, Nimrichter, Leonardo, Nolan, John P, Nolte-'t Hoen, Esther NM, Noren Hooten, Nicole, O'Driscoll, Lorraine, O'Grady, Tina, O'Loghlen, Ana, Ochiya, Takahiro, Olivier, Martin, Ortiz, Alberto, Ortiz, Luis A, Osteikoetxea, Xabier, Østergaard, Ole, Ostrowski, Matias, Park, Jaesung, Pegtel, D Michiel, Peinado, Hector, Perut, Francesca, Pfaffl, Michael W, Phinney, Donald G, Pieters, Bartijn Ch, Pink, Ryan C, Pisetsky, David S, Pogge von Strandmann, Elke, Polakovicova, Iva, Poon, Ivan Kh, Powell, Bonita H, Prada, Ilaria, Pulliam, Lynn, Quesenberry, Peter, Radeghieri, Annalisa, Raffai, Robert L, Raimondo, Stefania, Rak, Janusz, Ramirez, Marcel I, Raposo, Graça, Rayyan, Morsi S, Regev-Rudzki, Neta, Ricklefs, Franz L, Robbins, Paul D, Roberts, David D, Rodrigues, Silvia C, Rohde, Eva, Rome, Sophie, Rouschop, Kasper Ma, Rughetti, Aurelia, Russell, Ashley E, Saá, Paula, Sahoo, Susmita, Salas-Huenuleo, Edison, Sánchez, Catherine, Saugstad, Julie A, Saul, Meike J, Schiffelers, Raymond M, Schneider, Raphael, Schøyen, Tine Hiorth, Scott, Aaron, Shahaj, Eriomina, Sharma, Shivani, Shatnyeva, Olga, Shekari, Faezeh, Shelke, Ganesh Vilas, Shetty, Ashok K, Shiba, Kiyotaka, Siljander, Pia R-M, Silva, Andreia M, Skowronek, Agata, Snyder, Orman L, Soares, Rodrigo Pedro, Sódar, Barbara W, Soekmadji, Carolina, Sotillo, Javier, Stahl, Philip D, Stoorvogel, Willem, Stott, Shannon L, Strasser, Erwin F, Swift, Simon, Tahara, Hidetoshi, Tewari, Muneesh, Timms, Kate, Tiwari, Swasti, Tixeira, Rochelle, Tkach, Mercedes, Toh, Wei Seong, Tomasini, Richard, Torrecilhas, Ana Claudia, Tosar, Juan Pablo, Toxavidis, Vasilis, Urbanelli, Lorena, Vader, Pieter, van Balkom, Bas Wm, van der Grein, Susanne G, Van Deun, Jan, van Herwijnen, Martijn Jc, Van Keuren-Jensen, Kendall, van Niel, Guillaume, van Royen, Martin E, van Wijnen, Andre J, Vasconcelos, M Helena, Vechetti, Ivan J, Veit, Tiago D, Vella, Laura J, Velot, Émilie, Verweij, Frederik J, Vestad, Beate, Viñas, Jose L, Visnovitz, Tamás, Vukman, Krisztina V, Wahlgren, Jessica, Watson, Dionysios C, Wauben, Marca Hm, Weaver, Alissa, Webber, Jason P, Weber, Viktoria, Wehman, Ann M, Weiss, Daniel J, Welsh, Joshua A, Wendt, Sebastian, Wheelock, Asa M, Wiener, Zoltán, Witte, Leonie, Wolfram, Joy, Xagorari, Angeliki, Xander, Patricia, Xu, Jing, Yan, Xiaomei, Yáñez-Mó, María, Yin, Hang, Yuana, Yuana, Zappulli, Valentina, Zarubova, Jana, Žėkas, Vytautas, Zhang, Jian-Ye, Zhao, Zezhou, Zheng, Lei, Zheutlin, Alexander R, Zickler, Antje M, Zimmermann, Pascale, Zivkovic, Angela M, Zocco, Davide, Zuba-Surma, Ewa K, dB&C I&I, LS Celbiologie-Algemeen, Celbiologie, Afd Pharmaceutics, Sub General Pharmaceutics, Sub Biomol.Mass Spect. and Proteomics, Afd Pharmacology, Théry, Clotilde, Witwer, Kenneth W, Aikawa, Elena, Alcaraz, Maria Jose, Anderson, Johnathon D, Andriantsitohaina, Ramaroson, Antoniou, Anna, Arab, Tanina, Archer, Fabienne, Atkin-Smith, Georgia K, Ayre, D Craig, Bach, Jean-Marie, Bachurski, Daniel, Baharvand, Hossein, Balaj, Leonora, Baldacchino, Shawn, Bauer, Natalie N, Baxter, Amy A, Bebawy, Mary, Beckham, Carla, Bedina Zavec, Apolonija, Benmoussa, Abderrahim, Berardi, Anna C, Bergese, Paolo, Bielska, Ewa, Blenkiron, Cherie, Bobis-Wozowicz, Sylwia, Boilard, Eric, Boireau, Wilfrid, Bongiovanni, Antonella, Borràs, Francesc E, Bosch, Steffi, Boulanger, Chantal M, Breakefield, Xandra, Breglio, Andrew M, Brennan, Meadhbh Á, Brigstock, David R, Brisson, Alain, Broekman, Marike Ld, Bromberg, Jacqueline F, Bryl-Górecka, Paulina, Buch, Shilpa, Buck, Amy H, Burger, Dylan, Busatto, Sara, Buschmann, Dominik, Bussolati, Benedetta, Buzás, Edit I, Byrd, James Bryan, Camussi, Giovanni, Carter, David Rf, Caruso, Sarah, Chamley, Lawrence W, Chang, Yu-Ting, Chen, Chihchen, Chen, Shuai, Cheng, Lesley, Chin, Andrew R, Clayton, Aled, Clerici, Stefano P, Cocks, Alex, Cocucci, Emanuele, Coffey, Robert J, Cordeiro-da-Silva, Anabela, Couch, Yvonne, Coumans, Frank Aw, Coyle, Beth, Crescitelli, Rossella, Criado, Miria Ferreira, D'Souza-Schorey, Crislyn, Das, Saumya, Datta Chaudhuri, Amrita, de Candia, Paola, De Santana, Eliezer F, De Wever, Olivier, Del Portillo, Hernando A, Demaret, Tanguy, Deville, Sarah, Devitt, Andrew, Dhondt, Bert, Di Vizio, Dolores, Dieterich, Lothar C, Dolo, Vincenza, Dominguez Rubio, Ana Paula, Dominici, Massimo, Dourado, Mauricio R, Driedonks, Tom Ap, Duarte, Filipe V, Duncan, Heather M, Eichenberger, Ramon M, Ekström, Karin, El Andaloussi, Samir, Elie-Caille, Celine, Erdbrügger, Uta, Falcón-Pérez, Juan M, Fatima, Farah, Fish, Jason E, Flores-Bellver, Miguel, Försönits, András, Frelet-Barrand, Annie, Fricke, Fabia, Fuhrmann, Gregor, Gabrielsson, Susanne, Gámez-Valero, Ana, Gardiner, Chris, Gärtner, Kathrin, Gaudin, Raphael, Gho, Yong Song, Giebel, Bernd, Gilbert, Caroline, Gimona, Mario, Giusti, Ilaria, Goberdhan, Deborah Ci, Görgens, André, Gorski, Sharon M, Greening, David W, Gross, Julia Christina, Gualerzi, Alice, Gupta, Gopal N, Gustafson, Dakota, Handberg, Aase, Haraszti, Reka A, Harrison, Paul, Hegyesi, Hargita, Hendrix, An, Hill, Andrew F, Hochberg, Fred H, Hoffmann, Karl F, Holder, Beth, Holthofer, Harry, Hosseinkhani, Baharak, Hu, Guoku, Huang, Yiyao, Huber, Veronica, Hunt, Stuart, Ibrahim, Ahmed Gamal-Eldin, Ikezu, Tsuneya, Inal, Jameel M, Isin, Mustafa, Ivanova, Alena, Jackson, Hannah K, Jacobsen, Soren, Jay, Steven M, Jayachandran, Muthuvel, Jenster, Guido, Jiang, Lanzhou, Johnson, Suzanne M, Jones, Jennifer C, Jong, Ambrose, Jovanovic-Talisman, Tijana, Jung, Stephanie, Kalluri, Raghu, Kano, Shin-Ichi, Kaur, Sukhbir, Kawamura, Yumi, Keller, Evan T, Khamari, Delaram, Khomyakova, Elena, Khvorova, Anastasia, Kierulf, Peter, Kim, Kwang Pyo, Kislinger, Thomas, Klingeborn, Mikael, Klinke, David J, Kornek, Miroslaw, Kosanović, Maja M, Kovács, Árpád Ferenc, Krämer-Albers, Eva-Maria, Krasemann, Susanne, Krause, Mirja, Kurochkin, Igor V, Kusuma, Gina D, Kuypers, Sören, Laitinen, Saara, Langevin, Scott M, Languino, Lucia R, Lannigan, Joanne, Lässer, Cecilia, Laurent, Louise C, Lavieu, Gregory, Lázaro-Ibáñez, Elisa, Le Lay, Soazig, Lee, Myung-Shin, Lee, Yi Xin Fiona, Lemos, Debora S, Lenassi, Metka, Leszczynska, Aleksandra, Li, Isaac Ts, Liao, Ke, Libregts, Sten F, Ligeti, Erzsebet, Lim, Rebecca, Lim, Sai Kiang, Linē, Aija, Linnemannstöns, Karen, Llorente, Alicia, Lombard, Catherine A, Lorenowicz, Magdalena J, Lörincz, Ákos M, Lötvall, Jan, Lovett, Jason, Lowry, Michelle C, Loyer, Xavier, Lu, Quan, Lukomska, Barbara, Lunavat, Taral R, Maas, Sybren Ln, Malhi, Harmeet, Marcilla, Antonio, Mariani, Jacopo, Mariscal, Javier, Martens-Uzunova, Elena S, Martin-Jaular, Lorena, Martinez, M Carmen, Martins, Vilma Regina, Mathieu, Mathilde, Mathivanan, Suresh, Maugeri, Marco, McGinnis, Lynda K, McVey, Mark J, Meckes, David G, Meehan, Katie L, Mertens, Inge, Minciacchi, Valentina R, Möller, Andreas, Møller Jørgensen, Malene, Morales-Kastresana, Aizea, Morhayim, Jess, Mullier, François, Muraca, Maurizio, Musante, Luca, Mussack, Veronika, Muth, Dillon C, Myburgh, Kathryn H, Najrana, Tanbir, Nawaz, Muhammad, Nazarenko, Irina, Nejsum, Peter, Neri, Christian, Neri, Tommaso, Nieuwland, Rienk, Nimrichter, Leonardo, Nolan, John P, Nolte-'t Hoen, Esther NM, Noren Hooten, Nicole, O'Driscoll, Lorraine, O'Grady, Tina, O'Loghlen, Ana, Ochiya, Takahiro, Olivier, Martin, Ortiz, Alberto, Ortiz, Luis A, Osteikoetxea, Xabier, Østergaard, Ole, Ostrowski, Matias, Park, Jaesung, Pegtel, D Michiel, Peinado, Hector, Perut, Francesca, Pfaffl, Michael W, Phinney, Donald G, Pieters, Bartijn Ch, Pink, Ryan C, Pisetsky, David S, Pogge von Strandmann, Elke, Polakovicova, Iva, Poon, Ivan Kh, Powell, Bonita H, Prada, Ilaria, Pulliam, Lynn, Quesenberry, Peter, Radeghieri, Annalisa, Raffai, Robert L, Raimondo, Stefania, Rak, Janusz, Ramirez, Marcel I, Raposo, Graça, Rayyan, Morsi S, Regev-Rudzki, Neta, Ricklefs, Franz L, Robbins, Paul D, Roberts, David D, Rodrigues, Silvia C, Rohde, Eva, Rome, Sophie, Rouschop, Kasper Ma, Rughetti, Aurelia, Russell, Ashley E, Saá, Paula, Sahoo, Susmita, Salas-Huenuleo, Edison, Sánchez, Catherine, Saugstad, Julie A, Saul, Meike J, Schiffelers, Raymond M, Schneider, Raphael, Schøyen, Tine Hiorth, Scott, Aaron, Shahaj, Eriomina, Sharma, Shivani, Shatnyeva, Olga, Shekari, Faezeh, Shelke, Ganesh Vilas, Shetty, Ashok K, Shiba, Kiyotaka, Siljander, Pia R-M, Silva, Andreia M, Skowronek, Agata, Snyder, Orman L, Soares, Rodrigo Pedro, Sódar, Barbara W, Soekmadji, Carolina, Sotillo, Javier, Stahl, Philip D, Stoorvogel, Willem, Stott, Shannon L, Strasser, Erwin F, Swift, Simon, Tahara, Hidetoshi, Tewari, Muneesh, Timms, Kate, Tiwari, Swasti, Tixeira, Rochelle, Tkach, Mercedes, Toh, Wei Seong, Tomasini, Richard, Torrecilhas, Ana Claudia, Tosar, Juan Pablo, Toxavidis, Vasilis, Urbanelli, Lorena, Vader, Pieter, van Balkom, Bas Wm, van der Grein, Susanne G, Van Deun, Jan, van Herwijnen, Martijn Jc, Van Keuren-Jensen, Kendall, van Niel, Guillaume, van Royen, Martin E, van Wijnen, Andre J, Vasconcelos, M Helena, Vechetti, Ivan J, Veit, Tiago D, Vella, Laura J, Velot, Émilie, Verweij, Frederik J, Vestad, Beate, Viñas, Jose L, Visnovitz, Tamás, Vukman, Krisztina V, Wahlgren, Jessica, Watson, Dionysios C, Wauben, Marca Hm, Weaver, Alissa, Webber, Jason P, Weber, Viktoria, Wehman, Ann M, Weiss, Daniel J, Welsh, Joshua A, Wendt, Sebastian, Wheelock, Asa M, Wiener, Zoltán, Witte, Leonie, Wolfram, Joy, Xagorari, Angeliki, Xander, Patricia, Xu, Jing, Yan, Xiaomei, Yáñez-Mó, María, Yin, Hang, Yuana, Yuana, Zappulli, Valentina, Zarubova, Jana, Žėkas, Vytautas, Zhang, Jian-Ye, Zhao, Zezhou, Zheng, Lei, Zheutlin, Alexander R, Zickler, Antje M, Zimmermann, Pascale, Zivkovic, Angela M, Zocco, Davide, and Zuba-Surma, Ewa K
Published: 2018

64. Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017: Meeting report

Author: Clayton, Aled, Buschmann, Dominik, Byrd, J. Brian, Carter, David R.F., Chenge, Lesley, Compton, Carolyn, Daaboul, George, Devitt, Andrew, Falcon-Perez, Juan Manuel, Gardiner, Chris, Gustafson, Dakota, Harrison, Paul, Helmbrecht, Clemens, Hendrix, An, Hill, Andrew, Hoffman, Andrew, Jones, Jennifer C., Kalluri, Raghu, Kang, Ji Yoon, Kirchner, Benedikt, Lässert, Cecilia, Lawson, Charlotte, Lenassi, Metka, Levin, Carina, Llorente, Alicia, Martens-Uzunova, Elena S., Möller, Andreas, Musante, Luca, Ochiya, Takahiro, Pink, Ryan C., Tahara, Hidetoshi, Wauben, Marca H.M., Webber, Jason P., Welsh, Joshua A., Witwer, Kenneth W., Yin, Hang, Nieuwland, Rienk, Clayton, Aled, Buschmann, Dominik, Byrd, J. Brian, Carter, David R.F., Chenge, Lesley, Compton, Carolyn, Daaboul, George, Devitt, Andrew, Falcon-Perez, Juan Manuel, Gardiner, Chris, Gustafson, Dakota, Harrison, Paul, Helmbrecht, Clemens, Hendrix, An, Hill, Andrew, Hoffman, Andrew, Jones, Jennifer C., Kalluri, Raghu, Kang, Ji Yoon, Kirchner, Benedikt, Lässert, Cecilia, Lawson, Charlotte, Lenassi, Metka, Levin, Carina, Llorente, Alicia, Martens-Uzunova, Elena S., Möller, Andreas, Musante, Luca, Ochiya, Takahiro, Pink, Ryan C., Tahara, Hidetoshi, Wauben, Marca H.M., Webber, Jason P., Welsh, Joshua A., Witwer, Kenneth W., Yin, Hang, and Nieuwland, Rienk
Abstract: This report summarises the presentations and activities of the ISEV Workshop on extracellular vesicle biomarkers held in Birmingham, UK during December 2017. Among the key messages was broad agreement about the importance of biospecimen science. Much greater attention needs to be paid towards the provenance of collected samples. The workshop also highlighted clear gaps in our knowledge about pre-analytical factors that alter extracellular vesicles (EVs). The future utility of certified standards for credentialing of instruments and software, to analyse EV and for tracking the influence of isolation steps on the structure and content of EVs were also discussed. Several example studies were presented, demonstrating the potential utility for EVs in disease diagnosis, prognosis, longitudinal serial testing and stratification of patients. The conclusion of the workshop was that more effort focused on pre-analytical issues and benchmarking of isolation methods is needed to strengthen collaborations and advance more effective biomarkers.
Published: 2018

65. Dominant immunosuppression of dendritic cell function by prostate-cancer-derived exosomes

Author: Salimu, Josephine, Webber, Jason, Gurney, Mark, Al-Taei, Saly, Clayton, Aled, and Tabi, Zsuzsanna
Subjects: Prostate cancer, lcsh:Cytology, adenosine, antigen cross-presentation, Original Research Article, exosomes, PGE2, dendritic cells, lcsh:QH573-671, Research Article
Abstract: Exosomes are a distinct population of extracellular vesicles of endocytic origin with a protein repertoire similar to the parent cell. Although tumour-derived exosomes harbour immunosuppressive characteristics, they also carry tumour antigens and thus potentially contribute to immune activation. The aim of this study was to examine the impact of prostate cancer exosomes on tumour antigen cross-presentation. DU145 cells, transduced with shRNA to knockdown Rab27a (DU145KD) that inhibits exosome secretion, triggered significantly stronger tumour-antigen-specific T cell responses when loaded onto dendritic cells (DC) than control DU145 cells. Enhanced T cell response was prevented by adding purified exogenous DU145 exosomes to DU145KD cells, demonstrating that the dominant effect of tumour exosomes is immunosuppression and not antigen delivery. CD8+ T cell responses were impaired via exosomal regulation of DC function; exosomes triggered the expression of CD73, an ecto-5-nucleotidase responsible for AMP to adenosine hydrolysis, on DC. CD73 induction on DC that constitutively express CD39 resulted in an ATP-dependent inhibition of TNFα- and IL-12-production. We identified exosomal prostaglandin E2 (PGE2) as a potential driver of CD73 induction, as inhibition of PGE2 receptors significantly reduced exosome-dependent CD73 induction. The results reveal a hitherto unknown suppression of DC function via exosomal PGE2, adding a new element to tumour exosome–immune cell cross-talk. Abbreviations: AMP: adenosine monophosphate; ATP: adenosine triphosphate; BLCL: B lymphoblastoid cell line; CME: exosomes enriched from cell line conditioned media; DC: dendritic cell; DMSO: dimethyl-sulfoxide; DU145C: DU145 cells with irrelevant knockdown control; DU145KD: DU145 cells with Rab27a knockdown; ELISA: enzyme-linked immunosorbent assay; FBS: fetal bovine serum; GM-CSF: granulocyte-monocyte colony stimulating factor; HLA: human lymphocyte antigen; IL: interleukin; LPS: lipopolysaccharide; mfi: mean fluorescence intensity; PBMC: peripheral blood mononuclear cells; PBS: phosphate buffer solution; PGE2: prostaglandin E2; TRF: time-resolved fluorescence.
Published: 2017

66. Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017

Author: Clayton, Aled, primary, Buschmann, Dominik, additional, Byrd, J. Brian, additional, Carter, David R. F., additional, Cheng, Lesley, additional, Compton, Carolyn, additional, Daaboul, George, additional, Devitt, Andrew, additional, Falcon-Perez, Juan Manuel, additional, Gardiner, Chris, additional, Gustafson, Dakota, additional, Harrison, Paul, additional, Helmbrecht, Clemens, additional, Hendrix, An, additional, Hill, Andrew, additional, Hoffman, Andrew, additional, Jones, Jennifer C., additional, Kalluri, Raghu, additional, Kang, Ji Yoon, additional, Kirchner, Benedikt, additional, Lässer, Cecilia, additional, Lawson, Charlotte, additional, Lenassi, Metka, additional, Levin, Carina, additional, Llorente, Alicia, additional, Martens-Uzunova, Elena S., additional, Möller, Andreas, additional, Musante, Luca, additional, Ochiya, Takahiro, additional, Pink, Ryan C, additional, Tahara, Hidetoshi, additional, Wauben, Marca H. M., additional, Webber, Jason P., additional, Welsh, Joshua A., additional, Witwer, Kenneth W., additional, Yin, Hang, additional, and Nieuwland, Rienk, additional
Published: 2018
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67. Prostate cancer exosomes as modulators of the tumor microenvironment

Author: Shephard, Alex P., primary, Yeung, Vincent, additional, Clayton, Aled, additional, and Webber, Jason P., additional
Published: 2017
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68. A novel community driven software for functional enrichment analysis of extracellular vesicles data

Author: Pathan, Mohashin, primary, Keerthikumar, Shivakumar, additional, Chisanga, David, additional, Alessandro, Riccardo, additional, Ang, Ching‐Seng, additional, Askenase, Philip, additional, Batagov, Arsen O., additional, Benito‐Martin, Alberto, additional, Camussi, Giovanni, additional, Clayton, Aled, additional, Collino, Federica, additional, Di Vizio, Dolores, additional, Falcon‐Perez, Juan Manuel, additional, Fonseca, Pedro, additional, Fonseka, Pamali, additional, Fontana, Simona, additional, Gho, Yong Song, additional, Hendrix, An, additional, Hoen, Esther Nolte‐'t, additional, Iraci, Nunzio, additional, Kastaniegaard, Kenneth, additional, Kislinger, Thomas, additional, Kowal, Joanna, additional, Kurochkin, Igor V., additional, Leonardi, Tommaso, additional, Liang, Yaxuan, additional, Llorente, Alicia, additional, Lunavat, Taral R., additional, Maji, Sayantan, additional, Monteleone, Francesca, additional, Øverbye, Anders, additional, Panaretakis, Theocharis, additional, Patel, Tushar, additional, Peinado, Héctor, additional, Pluchino, Stefano, additional, Principe, Simona, additional, Ronquist, Goran, additional, Royo, Felix, additional, Sahoo, Susmita, additional, Spinelli, Cristiana, additional, Stensballe, Allan, additional, Théry, Clotilde, additional, Herwijnen, Martijn J.C., additional, Wauben, Marca, additional, Welton, Joanne L., additional, Zhao, Kening, additional, and Mathivanan, Suresh, additional
Published: 2017
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69. A novel community driven software for functional enrichment analysis of extracellular vesicles data

Author: Pathan, Mohashin, Keerthikumar, Shivakumar, Chisanga, David, Alessandro, Riccardo, Ang, Ching-Seng, Askenase, Philip, Batagov, Arsen O., Benito-Martin, Alberto, Camussi, Giovanni, Clayton, Aled, Collino, Federica, Di Vizio, Dolores, Falcon-Perez, JuanManuel, Fonseca, Pedro, Fonseka, Pamali, Fontana, Simona, Gho, Yong Song, Hendrix, An, Nolte-'t Hoen, Esther, Iraci, Nunzio, Kastaniegaard, Kenneth, Kislinger, Thomas, Kowal, Joanna, Kurochkin, Igor V., Leonardi, Tommaso, Liang, Yaxuan, Llorente, Alicia, Lunavat, Taral R., Maji, Sayantan, Monteleone, Francesca, Overbye, Anders, Panaretakis, Theocharis, Patel, Tushar, Peinado, Hector, Pluchino, Stefano, Principe, Simona, Ronquist, Göran, Royo, Felix, Sahoo, Susmita, Spinelli, Cristiana, Stensballe, Allan, Theory, Clotilde, van Herwijnen, Martijn J. C., Wauben, Marca, Welton, Joanne L., Zhao, Kening, Mathivanan, Suresh, Pathan, Mohashin, Keerthikumar, Shivakumar, Chisanga, David, Alessandro, Riccardo, Ang, Ching-Seng, Askenase, Philip, Batagov, Arsen O., Benito-Martin, Alberto, Camussi, Giovanni, Clayton, Aled, Collino, Federica, Di Vizio, Dolores, Falcon-Perez, JuanManuel, Fonseca, Pedro, Fonseka, Pamali, Fontana, Simona, Gho, Yong Song, Hendrix, An, Nolte-'t Hoen, Esther, Iraci, Nunzio, Kastaniegaard, Kenneth, Kislinger, Thomas, Kowal, Joanna, Kurochkin, Igor V., Leonardi, Tommaso, Liang, Yaxuan, Llorente, Alicia, Lunavat, Taral R., Maji, Sayantan, Monteleone, Francesca, Overbye, Anders, Panaretakis, Theocharis, Patel, Tushar, Peinado, Hector, Pluchino, Stefano, Principe, Simona, Ronquist, Göran, Royo, Felix, Sahoo, Susmita, Spinelli, Cristiana, Stensballe, Allan, Theory, Clotilde, van Herwijnen, Martijn J. C., Wauben, Marca, Welton, Joanne L., Zhao, Kening, and Mathivanan, Suresh
Abstract: Bioinformatics tools are imperative for the in depth analysis of heterogeneous high-throughput data. Most of the software tools are developed by specific laboratories or groups or companies wherein they are designed to perform the required analysis for the group. However, such software tools may fail to capture "what the community needs in a tool". Here, we describe a novel community-driven approach to build a comprehensive functional enrichment analysis tool. Using the existing FunRich tool as a template, we invited researchers to request additional features and/or changes. Remarkably, with the enthusiastic participation of the community, we were able to implement 90% of the requested features. FunRich enables plugin for extracellular vesicles wherein users can download and analyse data from Vesiclepedia database. By involving researchers early through community needs software development, we believe that comprehensive analysis tools can be developed in various scientific disciplines.
Published: 2017
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70. Extracellular vesicles in the tumour microenvironment

Author: Carter, David Raul Francisco, primary, Clayton, Aled, additional, Devitt, Andrew, additional, Hunt, Stuart, additional, and Lambert, Daniel W, additional
Published: 2017
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71. Differential Proteome Analysis of Extracellular Vesicles from Breast Cancer Cell Lines by Chaperone Affinity Enrichment

Author: Griffiths, Steven, primary, Cormier, Michelle, additional, Clayton, Aled, additional, and Doucette, Alan, additional
Published: 2017
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72. Cerebrospinal fluid extracellular vesicle enrichment for protein biomarker discovery in neurological disease; multiple sclerosis

Author: Welton, Joanne L., primary, Loveless, Samantha, additional, Stone, Timothy, additional, von Ruhland, Chris, additional, Robertson, Neil P., additional, and Clayton, Aled, additional
Published: 2017
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73. Resistance to HER2-targeted anti-cancer drugs is associated with immune evasion in cancer cells and their derived extracellular vesicles

Author: Martinez, Vanesa G., primary, O'Neill, Sadhbh, additional, Salimu, Josephine, additional, Breslin, Susan, additional, Clayton, Aled, additional, Crown, John, additional, and O'Driscoll, Lorraine, additional
Published: 2017
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74. Prostaglandin E2-mediated adenosinergic effects on CD14+cells: Self-amplifying immunosuppression in cancer

Author: Al-Taei, Saly, primary, Salimu, Josephine, additional, Spary, Lisa K., additional, Clayton, Aled, additional, Lester, Jason F., additional, and Tabi, Zsuzsanna, additional
Published: 2017
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75. EVpedia: a community web portal for extracellular vesicles research

Author: Kim, Dae-Kyum, Lee, Jaewook, Kim, Sae Rom, Choi, Dong-Sic, Yoon, Yae Jin, Kim, Ji Hyun, Go, Gyeongyun, Nhung, Dinh, Hong, Kahye, Jang, Su Chul, Kim, Si-Hyun, Park, Kyong-Su, Kim, Oh Youn, Park, Hyun Taek, Seo, Ji Hye, Aikawa, Elena, Baj-Krzyworzeka, Monika, van Balkom, Bas W M, Belting, Mattias, Blanc, Lionel, Bond, Vincent, Bongiovanni, Antonella, Borràs, Francesc E, Buée, Luc, Buzás, Edit I, Cheng, Lesley, Clayton, Aled, Cocucci, Emanuele, Dela Cruz, Charles S, Desiderio, Dominic M, Di Vizio, Dolores, Ekström, Karin, Falcon-Perez, Juan M, Gardiner, Chris, Giebel, Bernd, Greening, David W, Gross, Julia Christina, Gupta, Dwijendra, Hendrix, An, Hill, Andrew F, Hill, Michelle M, Nolte-'t Hoen, Esther, Hwang, Do Won, Inal, Jameel, Jagannadham, Medicharla V, Jayachandran, Muthuvel, Jee, Young-Koo, Jørgensen, Malene, Kim, Kwang Pyo, Kim, Yoon-Keun, Kislinger, Thomas, Lässer, Cecilia, Lee, Dong Soo, Lee, Hakmo, van Leeuwen, Johannes, Lener, Thomas, Liu, Ming-Lin, Lötvall, Jan, Marcilla, Antonio, Mathivanan, Suresh, Möller, Andreas, Morhayim, Jess, Mullier, François, Nazarenko, Irina, Nieuwland, Rienk, Nunes, Diana N, Pang, Ken, Park, Jaesung, Patel, Tushar, Pocsfalvi, Gabriella, Del Portillo, Hernando, Putz, Ulrich, Ramirez, Marcel I, Rodrigues, Marcio L, Roh, Tae-Young, Royo, Felix, Sahoo, Susmita, Schiffelers, Raymond, Sharma, Shivani, Siljander, Pia, Simpson, Richard J, Soekmadji, Carolina, Stahl, Philip, Stensballe, Allan, Stępień, Ewa, Tahara, Hidetoshi, Trummer, Arne, Valadi, Hadi, Vella, Laura J, Wai, Sun Nyunt, Witwer, Kenneth, Yáñez-Mó, María, Youn, Hyewon, Zeidler, Reinhard, Gho, Yong Song, Nolte - t Hoen, Esther, Biology of Reproductive Cells, Strategic Infection Biology, Sub General Pharmaceutics, LS Celbiologie-Algemeen, and B&C BRC-SIB
Abstract: MOTIVATION: Extracellular vesicles (EVs) are spherical bilayered proteolipids, harboring various bioactive molecules. Due to the complexity of the vesicular nomenclatures and components, online searches for EV-related publications and vesicular components are currently challenging. RESULTS: We present an improved version of EVpedia, a public database for EVs research. This community web portal contains a database of publications and vesicular components, identification of orthologous vesicular components, bioinformatic tools and a personalized function. EVpedia includes 6879 publications, 172 080 vesicular components from 263 high-throughput datasets, and has been accessed more than 65 000 times from more than 750 cities. In addition, about 350 members from 73 international research groups have participated in developing EVpedia. This free web-based database might serve as a useful resource to stimulate the emerging field of EV research. Availability and implementation: The web site was implemented in PHP, Java, MySQL and Apache, and is freely available at http://evpedia.info. CONTACT: ysgho@postech.ac.kr.
Published: 2014

76. Coronary Artery-Bypass-Graft Surgery Increases the Plasma Concentration of Exosomes Carrying a Cargo of Cardiac MicroRNAs: An Example of Exosome Trafficking Out of the Human Heart with Potential for Cardiac Biomarker Discovery

Author: Emanueli, Costanza, primary, Shearn, Andrew I. U., additional, Laftah, Abas, additional, Fiorentino, Francesca, additional, Reeves, Barnaby C., additional, Beltrami, Cristina, additional, Mumford, Andrew, additional, Clayton, Aled, additional, Gurney, Mark, additional, Shantikumar, Saran, additional, and Angelini, Gianni D., additional
Published: 2016
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77. Prostate stromal cell proteomics analysis discriminates normal from tumour reactive stromal phenotypes

Author: Webber, Jason P., primary, Spary, Lisa K., additional, Mason, Malcolm D., additional, Tabi, Zsuzsanna, additional, Brewis, Ian A., additional, and Clayton, Aled, additional
Published: 2016
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78. Proteomics analysis of vesicles isolated from plasma and urine of prostate cancer patients using a multiplex, aptamer-based protein array

Author: Welton, Joanne Louise, primary, Brennan, Paul, additional, Gurney, Mark, additional, Webber, Jason Paul, additional, Spary, Lisa Kate, additional, Carton, David Gil, additional, Falcón-Pérez, Juan Manuel, additional, Walton, Sean Peter, additional, Mason, Malcolm David, additional, Tabi, Zsuzsanna, additional, and Clayton, Aled, additional
Published: 2016
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79. EVpedia : A community web portal for extracellular vesicles research

Author: Kim, Dae Kyum, Lee, Jaewook, Kim, Sae Rom, Choi, Dong Sic, Yoon, Yae Jin, Kim, Ji Hyun, Go, Gyeongyun, Nhung, Dinh, Hong, Kahye, Jang, Su Chul, Kim, Si Hyun, Park, Kyong Su, Kim, Oh Youn, Park, Hyun Taek, Seo, Ji Hye, Aikawa, Elena, Baj-Krzyworzeka, Monika, Van Balkom, Bas W M, Belting, Mattias, Blanc, Lionel, Bond, Vincent, Bongiovanni, Antonella, Borràs, Francesc E., Buée, Luc, Buzás, Edit I., Cheng, Lesley, Clayton, Aled, Cocucci, Emanuele, Dela Cruz, Charles S., Desiderio, Dominic M., Di Vizio, Dolores, Ekström, Karin, Falcon-Perez, Juan M., Gardiner, Chris, Giebel, Bernd, Greening, David W., Christina Gross, Julia, Gupta, Dwijendra, Hendrix, An, Hill, Andrew F., Hill, Michelle M., Nolte-'T Hoen, Esther, Hwang, Do Won, Inal, Jameel, Jagannadham, Medicharla V., Jayachandran, Muthuvel, Jee, Young Koo, Jørgensen, Malene, Kim, Kwang Pyo, Kim, Yoon Keun, Kislinger, Thomas, Lässer, Cecilia, Lee, Dong Soo, Lee, Hakmo, Van Leeuwen, Johannes, Lener, Thomas, Liu, Ming Lin, Lötvall, Jan, Marcilla, Antonio, Mathivanan, Suresh, Möller, Andreas, Morhayim, Jess, Mullier, Francois, Nazarenko, Irina, Nieuwland, Rienk, Nunes, Diana N., Pang, Ken, Park, Jaesung, Patel, Tushar, Pocsfalvi, Gabriella, Del Portillo, Hernando, Putz, Ulrich, Ramirez, Marcel I., Rodrigues, Marcio L., Roh, Tae Young, Royo, Felix, Sahoo, Susmita, Schiffelers, Raymond, Sharma, Shivani, Siljander, Pia, Simpson, Richard J., Soekmadji, Carolina, Stahl, Philip, Stensballe, Allan, Stepień, Ewa, Tahara, Hidetoshi, Trummer, Arne, Valadi, Hadi, Vella, Laura J., Wai, Sun Nyunt, Witwer, Kenneth, Yánez-Mó, Maria, Youn, Hyewon, Zeidler, Reinhard, Gho, Yong Song, Kim, Dae Kyum, Lee, Jaewook, Kim, Sae Rom, Choi, Dong Sic, Yoon, Yae Jin, Kim, Ji Hyun, Go, Gyeongyun, Nhung, Dinh, Hong, Kahye, Jang, Su Chul, Kim, Si Hyun, Park, Kyong Su, Kim, Oh Youn, Park, Hyun Taek, Seo, Ji Hye, Aikawa, Elena, Baj-Krzyworzeka, Monika, Van Balkom, Bas W M, Belting, Mattias, Blanc, Lionel, Bond, Vincent, Bongiovanni, Antonella, Borràs, Francesc E., Buée, Luc, Buzás, Edit I., Cheng, Lesley, Clayton, Aled, Cocucci, Emanuele, Dela Cruz, Charles S., Desiderio, Dominic M., Di Vizio, Dolores, Ekström, Karin, Falcon-Perez, Juan M., Gardiner, Chris, Giebel, Bernd, Greening, David W., Christina Gross, Julia, Gupta, Dwijendra, Hendrix, An, Hill, Andrew F., Hill, Michelle M., Nolte-'T Hoen, Esther, Hwang, Do Won, Inal, Jameel, Jagannadham, Medicharla V., Jayachandran, Muthuvel, Jee, Young Koo, Jørgensen, Malene, Kim, Kwang Pyo, Kim, Yoon Keun, Kislinger, Thomas, Lässer, Cecilia, Lee, Dong Soo, Lee, Hakmo, Van Leeuwen, Johannes, Lener, Thomas, Liu, Ming Lin, Lötvall, Jan, Marcilla, Antonio, Mathivanan, Suresh, Möller, Andreas, Morhayim, Jess, Mullier, Francois, Nazarenko, Irina, Nieuwland, Rienk, Nunes, Diana N., Pang, Ken, Park, Jaesung, Patel, Tushar, Pocsfalvi, Gabriella, Del Portillo, Hernando, Putz, Ulrich, Ramirez, Marcel I., Rodrigues, Marcio L., Roh, Tae Young, Royo, Felix, Sahoo, Susmita, Schiffelers, Raymond, Sharma, Shivani, Siljander, Pia, Simpson, Richard J., Soekmadji, Carolina, Stahl, Philip, Stensballe, Allan, Stepień, Ewa, Tahara, Hidetoshi, Trummer, Arne, Valadi, Hadi, Vella, Laura J., Wai, Sun Nyunt, Witwer, Kenneth, Yánez-Mó, Maria, Youn, Hyewon, Zeidler, Reinhard, and Gho, Yong Song
Published: 2015

80. Classification of current anticancer immunotherapies

Author: UCL - SSS/DDUV - Institut de Duve, Galluzzi, Lorenzo, Vacchelli, Erika, Bravo-San Pedro, José-Manuel, Buqué, Aitziber, Senovilla, Laura, Baracco, Elisa Elena, Bloy, Norma, Castoldi, Francesca, Abastado, Jean-Pierre, Agostinis, Patrizia, Apte, Ron N, Aranda, Fernando, Ayyoub, Maha, Beckhove, Philipp, Blay, Jean-Yves, Bracci, Laura, Caignard, Anne, Castelli, Chiara, Cavallo, Federica, Celis, Estaban, Cerundolo, Vincenzo, Clayton, Aled, Colombo, Mario P, Coussens, Lisa, Dhodapkar, Madhav V, Eggermont, Alexander M, Fearon, Douglas T, Wolf H, Fridman, Fucikova, Jitka, Gabrilovitch, Dmitry I, Galon, Jérôme, Garg, Abhishek, Ghiringhelli, François, Giaccone, Giuseppe, Gilboa, Eli, Gnjatic, Sacha, Hoos, Axel, Hosmalin, Anne, Jäger, Dirk, Kalinski, Pawel, Kärre, Klas, Kepp, Oliver, Kiessling, Rolf, Kirkwood, John M, Klein, Eva, Knuth, Alexander, Lewis, Claire E, Libiau, Roland, Lotze, Michael T, Lugli, Enrico, Mach, Jean-Pierre, Mattei, Fabrizio, Mavillo, Domenico, Melero, Ignacio, Melief, Cornelis J, Mittendorf, Elizabeth A, Moretta, Lorenzo, Odunsi, Adekunke, Okada, Hideho, Palucka, Anna Karolina, Peter, Marcus E, Pienta, Kenneth J, Porgador, Angel, Prendergast, George C, Rabinovich, Gabriel A, Restifo, Nicholas P, Rizvi, Naiyer, Sautès-Fridman, Catherine, Schreiber, Hans, Seliger, Barbara, Hiroshi, Shiku, Silva-Santos, Bruno, Smyth, Mark J, Speiser, Daniel E, Spisek, Radek, Srivastava, Pramod K, Taimadge, James E, Tartour, Eric, Van Der Burg, Sjoerd H, Van den Eynde, Benoît, Vile, Richard, Wagner, Hermann, Weber, Jeffrey S, Whiteside, Theresa L, Wolchok, Jedd D, Zitvogel, Laurence, Zou, Weiping, Kroemer, Guido, UCL - SSS/DDUV - Institut de Duve, Galluzzi, Lorenzo, Vacchelli, Erika, Bravo-San Pedro, José-Manuel, Buqué, Aitziber, Senovilla, Laura, Baracco, Elisa Elena, Bloy, Norma, Castoldi, Francesca, Abastado, Jean-Pierre, Agostinis, Patrizia, Apte, Ron N, Aranda, Fernando, Ayyoub, Maha, Beckhove, Philipp, Blay, Jean-Yves, Bracci, Laura, Caignard, Anne, Castelli, Chiara, Cavallo, Federica, Celis, Estaban, Cerundolo, Vincenzo, Clayton, Aled, Colombo, Mario P, Coussens, Lisa, Dhodapkar, Madhav V, Eggermont, Alexander M, Fearon, Douglas T, Wolf H, Fridman, Fucikova, Jitka, Gabrilovitch, Dmitry I, Galon, Jérôme, Garg, Abhishek, Ghiringhelli, François, Giaccone, Giuseppe, Gilboa, Eli, Gnjatic, Sacha, Hoos, Axel, Hosmalin, Anne, Jäger, Dirk, Kalinski, Pawel, Kärre, Klas, Kepp, Oliver, Kiessling, Rolf, Kirkwood, John M, Klein, Eva, Knuth, Alexander, Lewis, Claire E, Libiau, Roland, Lotze, Michael T, Lugli, Enrico, Mach, Jean-Pierre, Mattei, Fabrizio, Mavillo, Domenico, Melero, Ignacio, Melief, Cornelis J, Mittendorf, Elizabeth A, Moretta, Lorenzo, Odunsi, Adekunke, Okada, Hideho, Palucka, Anna Karolina, Peter, Marcus E, Pienta, Kenneth J, Porgador, Angel, Prendergast, George C, Rabinovich, Gabriel A, Restifo, Nicholas P, Rizvi, Naiyer, Sautès-Fridman, Catherine, Schreiber, Hans, Seliger, Barbara, Hiroshi, Shiku, Silva-Santos, Bruno, Smyth, Mark J, Speiser, Daniel E, Spisek, Radek, Srivastava, Pramod K, Taimadge, James E, Tartour, Eric, Van Der Burg, Sjoerd H, Van den Eynde, Benoît, Vile, Richard, Wagner, Hermann, Weber, Jeffrey S, Whiteside, Theresa L, Wolchok, Jedd D, Zitvogel, Laurence, Zou, Weiping, and Kroemer, Guido
Abstract: During the past decades, anticancer immunotherapy has evolved from a promising therapeutic option to a robust clinical reality. Many immunotherapeutic regimens are now approved by the US Food and Drug Administration and the European Medicines Agency for use in cancer patients, and many others are being investigated as standalone therapeutic interventions or combined with conventional treatments in clinical studies. Immunotherapies may be subdivided into “passive” and “active” based on their ability to engage the host immune system against cancer. Since the anticancer activity of most passive immunotherapeutics (including tumor-targeting monoclonal antibodies) also relies on the host immune system, this classification does not properly reflect the complexity of the drug-host-tumor interaction. Alternatively, anticancer immunotherapeutics can be classified according to their antigen specificity. While some immunotherapies specifically target one (or a few) defined tumor-associated antigen(s), others operate in a relatively non-specific manner and boost natural or therapy-elicited anticancer immune responses of unknown and often broad specificity. Here, we propose a critical, integrated classification of anticancer immunotherapies and discuss the clinical relevance of these approaches.
Published: 2015

81. EVpedia: A community web portal for extracellular vesicles research

Author: MS Nefrologie, Circulatory Health, CDL Staf Research, Kim, Dae Kyum, Lee, Jaewook, Kim, Sae Rom, Choi, Dong Sic, Yoon, Yae Jin, Kim, Ji Hyun, Go, Gyeongyun, Nhung, Dinh, Hong, Kahye, Jang, Su Chul, Kim, Si Hyun, Park, Kyong Su, Kim, Oh Youn, Park, Hyun Taek, Seo, Ji Hye, Aikawa, Elena, Baj-Krzyworzeka, Monika, Van Balkom, Bas W M, Belting, Mattias, Blanc, Lionel, Bond, Vincent, Bongiovanni, Antonella, Borràs, Francesc E., Buée, Luc, Buzás, Edit I., Cheng, Lesley, Clayton, Aled, Cocucci, Emanuele, Dela Cruz, Charles S., Desiderio, Dominic M., Di Vizio, Dolores, Ekström, Karin, Falcon-Perez, Juan M., Gardiner, Chris, Giebel, Bernd, Greening, David W., Christina Gross, Julia, Gupta, Dwijendra, Hendrix, An, Hill, Andrew F., Hill, Michelle M., Nolte-'T Hoen, Esther, Hwang, Do Won, Inal, Jameel, Jagannadham, Medicharla V., Jayachandran, Muthuvel, Jee, Young Koo, Jørgensen, Malene, Kim, Kwang Pyo, Kim, Yoon Keun, Kislinger, Thomas, Lässer, Cecilia, Lee, Dong Soo, Lee, Hakmo, Van Leeuwen, Johannes, Lener, Thomas, Liu, Ming Lin, Lötvall, Jan, Marcilla, Antonio, Mathivanan, Suresh, Möller, Andreas, Morhayim, Jess, Mullier, Francois, Nazarenko, Irina, Nieuwland, Rienk, Nunes, Diana N., Pang, Ken, Park, Jaesung, Patel, Tushar, Pocsfalvi, Gabriella, Del Portillo, Hernando, Putz, Ulrich, Ramirez, Marcel I., Rodrigues, Marcio L., Roh, Tae Young, Royo, Felix, Sahoo, Susmita, Schiffelers, Raymond, Sharma, Shivani, Siljander, Pia, Simpson, Richard J., Soekmadji, Carolina, Stahl, Philip, Stensballe, Allan, Stepień, Ewa, Tahara, Hidetoshi, Trummer, Arne, Valadi, Hadi, Vella, Laura J., Wai, Sun Nyunt, Witwer, Kenneth, Yánez-Mó, Maria, Youn, Hyewon, Zeidler, Reinhard, Gho, Yong Song, MS Nefrologie, Circulatory Health, CDL Staf Research, Kim, Dae Kyum, Lee, Jaewook, Kim, Sae Rom, Choi, Dong Sic, Yoon, Yae Jin, Kim, Ji Hyun, Go, Gyeongyun, Nhung, Dinh, Hong, Kahye, Jang, Su Chul, Kim, Si Hyun, Park, Kyong Su, Kim, Oh Youn, Park, Hyun Taek, Seo, Ji Hye, Aikawa, Elena, Baj-Krzyworzeka, Monika, Van Balkom, Bas W M, Belting, Mattias, Blanc, Lionel, Bond, Vincent, Bongiovanni, Antonella, Borràs, Francesc E., Buée, Luc, Buzás, Edit I., Cheng, Lesley, Clayton, Aled, Cocucci, Emanuele, Dela Cruz, Charles S., Desiderio, Dominic M., Di Vizio, Dolores, Ekström, Karin, Falcon-Perez, Juan M., Gardiner, Chris, Giebel, Bernd, Greening, David W., Christina Gross, Julia, Gupta, Dwijendra, Hendrix, An, Hill, Andrew F., Hill, Michelle M., Nolte-'T Hoen, Esther, Hwang, Do Won, Inal, Jameel, Jagannadham, Medicharla V., Jayachandran, Muthuvel, Jee, Young Koo, Jørgensen, Malene, Kim, Kwang Pyo, Kim, Yoon Keun, Kislinger, Thomas, Lässer, Cecilia, Lee, Dong Soo, Lee, Hakmo, Van Leeuwen, Johannes, Lener, Thomas, Liu, Ming Lin, Lötvall, Jan, Marcilla, Antonio, Mathivanan, Suresh, Möller, Andreas, Morhayim, Jess, Mullier, Francois, Nazarenko, Irina, Nieuwland, Rienk, Nunes, Diana N., Pang, Ken, Park, Jaesung, Patel, Tushar, Pocsfalvi, Gabriella, Del Portillo, Hernando, Putz, Ulrich, Ramirez, Marcel I., Rodrigues, Marcio L., Roh, Tae Young, Royo, Felix, Sahoo, Susmita, Schiffelers, Raymond, Sharma, Shivani, Siljander, Pia, Simpson, Richard J., Soekmadji, Carolina, Stahl, Philip, Stensballe, Allan, Stepień, Ewa, Tahara, Hidetoshi, Trummer, Arne, Valadi, Hadi, Vella, Laura J., Wai, Sun Nyunt, Witwer, Kenneth, Yánez-Mó, Maria, Youn, Hyewon, Zeidler, Reinhard, and Gho, Yong Song
Published: 2015

82. How pure are your vesicles?

Author: Clayton, Aled, Webber, Jason, and This work was supported by a Cancer Research Wales programme grant awarded to AC.
Subjects: Extracellular Vesicles, Nano-particle tracking, vesicle counting, sample purity
Abstract: We propose a straightforward method to estimate the purity of vesicle preparations by comparing the ratio of nano-vesicle counts to protein concentration, using tools such as the increasingly available NanoSight platform and a colorimetric protein assay such as the BCA-assay. Such an approach is simple enough to apply to every vesicle preparation within a given laboratory, assisting researchers as a routine quality control step. Also, the approach may aid in comparing/standardising vesicle purity across diverse studies, and may be of particular importance in evaluating vesicular biomarkers. We herein propose some criteria to aid in the definition of pure vesicles.Keywords: extracellular vesicles; nano-particle tracking; vesicle counting; sample purity(Published: 14 January 2013)Citation: Journal of Extracellular Vesicles 2013, 2: 19861 - http://dx.doi.org/10.3402/jev.v2i0.19861
Published: 2013

83. Stabilization of Urinary MicroRNAs by Association with Exosomes and Argonaute 2 Protein

Author: Beltrami, Cristina, primary, Clayton, Aled, additional, Newbury, Lucy, additional, Corish, Peter, additional, Jenkins, Robert, additional, Phillips, Aled, additional, Fraser, Donald, additional, and Bowen, Timothy, additional
Published: 2015
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84. Cross-Presentation of the Oncofetal Tumor Antigen 5T4 from Irradiated Prostate Cancer Cells—A Key Role for Heat-Shock Protein 70 and Receptor CD91

Author: Salimu, Josephine, primary, Spary, Lisa K., additional, Al-Taei, Saly, additional, Clayton, Aled, additional, Mason, Malcolm D., additional, Staffurth, John, additional, and Tabi, Zsuzsanna, additional
Published: 2015
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85. Extracellular vesicles as modulators of the cancer microenvironment

Author: Webber, Jason, primary, Yeung, Vincent, additional, and Clayton, Aled, additional
Published: 2015
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86. Characterisation of adipocyte-derived extracellular vesicles released pre- and post-adipogenesis

Author: Connolly, Katherine D., primary, Guschina, Irina A., additional, Yeung, Vincent, additional, Clayton, Aled, additional, Draman, Mohd Shazli, additional, Von Ruhland, Christopher, additional, Ludgate, Marian, additional, James, Philip E., additional, and Rees, D. Aled, additional
Published: 2015
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87. Ready-made chromatography columns for extracellular vesicle isolation from plasma

Author: Welton, Joanne Louise, primary, Webber, Jason Paul, additional, Botos, Laur-Alexandru, additional, Jones, Michael, additional, and Clayton, Aled, additional
Published: 2015
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88. EVpedia: a community web portal for extracellular vesicles research

Author: Biology of Reproductive Cells, Strategic Infection Biology, Sub General Pharmaceutics, LS Celbiologie-Algemeen, B&C BRC-SIB, Kim, Dae-Kyum, Lee, Jaewook, Kim, Sae Rom, Choi, Dong-Sic, Yoon, Yae Jin, Kim, Ji Hyun, Go, Gyeongyun, Nhung, Dinh, Hong, Kahye, Jang, Su Chul, Kim, Si-Hyun, Park, Kyong-Su, Kim, Oh Youn, Park, Hyun Taek, Seo, Ji Hye, Aikawa, Elena, Baj-Krzyworzeka, Monika, van Balkom, Bas W M, Belting, Mattias, Blanc, Lionel, Bond, Vincent, Bongiovanni, Antonella, Borràs, Francesc E, Buée, Luc, Buzás, Edit I, Cheng, Lesley, Clayton, Aled, Cocucci, Emanuele, Dela Cruz, Charles S, Desiderio, Dominic M, Di Vizio, Dolores, Ekström, Karin, Falcon-Perez, Juan M, Gardiner, Chris, Giebel, Bernd, Greening, David W, Gross, Julia Christina, Gupta, Dwijendra, Hendrix, An, Hill, Andrew F, Hill, Michelle M, Nolte-'t Hoen, Esther, Hwang, Do Won, Inal, Jameel, Jagannadham, Medicharla V, Jayachandran, Muthuvel, Jee, Young-Koo, Jørgensen, Malene, Kim, Kwang Pyo, Kim, Yoon-Keun, Kislinger, Thomas, Lässer, Cecilia, Lee, Dong Soo, Lee, Hakmo, van Leeuwen, Johannes, Lener, Thomas, Liu, Ming-Lin, Lötvall, Jan, Marcilla, Antonio, Mathivanan, Suresh, Möller, Andreas, Morhayim, Jess, Mullier, François, Nazarenko, Irina, Nieuwland, Rienk, Nunes, Diana N, Pang, Ken, Park, Jaesung, Patel, Tushar, Pocsfalvi, Gabriella, Del Portillo, Hernando, Putz, Ulrich, Ramirez, Marcel I, Rodrigues, Marcio L, Roh, Tae-Young, Royo, Felix, Sahoo, Susmita, Schiffelers, Raymond, Sharma, Shivani, Siljander, Pia, Simpson, Richard J, Soekmadji, Carolina, Stahl, Philip, Stensballe, Allan, Stępień, Ewa, Tahara, Hidetoshi, Trummer, Arne, Valadi, Hadi, Vella, Laura J, Wai, Sun Nyunt, Witwer, Kenneth, Yáñez-Mó, María, Youn, Hyewon, Zeidler, Reinhard, Gho, Yong Song, Nolte - t Hoen, Esther, Biology of Reproductive Cells, Strategic Infection Biology, Sub General Pharmaceutics, LS Celbiologie-Algemeen, B&C BRC-SIB, Kim, Dae-Kyum, Lee, Jaewook, Kim, Sae Rom, Choi, Dong-Sic, Yoon, Yae Jin, Kim, Ji Hyun, Go, Gyeongyun, Nhung, Dinh, Hong, Kahye, Jang, Su Chul, Kim, Si-Hyun, Park, Kyong-Su, Kim, Oh Youn, Park, Hyun Taek, Seo, Ji Hye, Aikawa, Elena, Baj-Krzyworzeka, Monika, van Balkom, Bas W M, Belting, Mattias, Blanc, Lionel, Bond, Vincent, Bongiovanni, Antonella, Borràs, Francesc E, Buée, Luc, Buzás, Edit I, Cheng, Lesley, Clayton, Aled, Cocucci, Emanuele, Dela Cruz, Charles S, Desiderio, Dominic M, Di Vizio, Dolores, Ekström, Karin, Falcon-Perez, Juan M, Gardiner, Chris, Giebel, Bernd, Greening, David W, Gross, Julia Christina, Gupta, Dwijendra, Hendrix, An, Hill, Andrew F, Hill, Michelle M, Nolte-'t Hoen, Esther, Hwang, Do Won, Inal, Jameel, Jagannadham, Medicharla V, Jayachandran, Muthuvel, Jee, Young-Koo, Jørgensen, Malene, Kim, Kwang Pyo, Kim, Yoon-Keun, Kislinger, Thomas, Lässer, Cecilia, Lee, Dong Soo, Lee, Hakmo, van Leeuwen, Johannes, Lener, Thomas, Liu, Ming-Lin, Lötvall, Jan, Marcilla, Antonio, Mathivanan, Suresh, Möller, Andreas, Morhayim, Jess, Mullier, François, Nazarenko, Irina, Nieuwland, Rienk, Nunes, Diana N, Pang, Ken, Park, Jaesung, Patel, Tushar, Pocsfalvi, Gabriella, Del Portillo, Hernando, Putz, Ulrich, Ramirez, Marcel I, Rodrigues, Marcio L, Roh, Tae-Young, Royo, Felix, Sahoo, Susmita, Schiffelers, Raymond, Sharma, Shivani, Siljander, Pia, Simpson, Richard J, Soekmadji, Carolina, Stahl, Philip, Stensballe, Allan, Stępień, Ewa, Tahara, Hidetoshi, Trummer, Arne, Valadi, Hadi, Vella, Laura J, Wai, Sun Nyunt, Witwer, Kenneth, Yáñez-Mó, María, Youn, Hyewon, Zeidler, Reinhard, Gho, Yong Song, and Nolte - t Hoen, Esther
Published: 2014

89. Classification of current anticancer immunotherapies.

Author: Galluzzi, Lorenzo, Vacchelli, Erika, Bravo-San Pedro, José-Manuel, Buqué, Aitziber, Senovilla, Laura, Baracco, Elisa Elena, Bloy, Norma, Castoldi, Francesca, Abastado, Jean-Pierre, Agostinis, Patrizia, Apte, Ron N, Aranda, Fernando, Ayyoub, Maha, Beckhove, Philipp, Blay, Jean-Yves, Bracci, Laura, Caignard, Anne, Castelli, Chiara, Cavallo, Federica, Celis, Estaban, Cerundolo, Vincenzo, Clayton, Aled, Colombo, Mario P, Coussens, Lisa, Dhodapkar, Madhav V, Eggermont, Alexander M, Fearon, Douglas T, Fridman, Wolf H, Fučíková, Jitka, Gabrilovich, Dmitry I, Galon, Jérôme, Garg, Abhishek, Ghiringhelli, François, Giaccone, Giuseppe, Gilboa, Eli, Gnjatic, Sacha, Hoos, Axel, Hosmalin, Anne, Jäger, Dirk, Kalinski, Pawel, Kärre, Klas, Kepp, Oliver, Kiessling, Rolf, Kirkwood, John M, Klein, Eva, Knuth, Alexander, Lewis, Claire E, Liblau, Roland, Lotze, Michael T, Lugli, Enrico, Mach, Jean-Pierre, Mattei, Fabrizio, Mavilio, Domenico, Melero, Ignacio, Melief, Cornelis J, Mittendorf, Elizabeth A, Moretta, Lorenzo, Odunsi, Adekunke, Okada, Hideho, Palucka, Anna Karolina, Peter, Marcus E, Pienta, Kenneth J, Porgador, Angel, Prendergast, George C, Rabinovich, Gabriel A, Restifo, Nicholas P, Rizvi, Naiyer, Sautès-Fridman, Catherine, Schreiber, Hans, Seliger, Barbara, Shiku, Hiroshi, Silva-Santos, Bruno, Smyth, Mark J, Speiser, Daniel E, Spisek, Radek, Srivastava, Pramod K, Talmadge, James E, Tartour, Eric, Van Der Burg, Sjoerd H, Van Den Eynde, Benoît J, Vile, Richard, Wagner, Hermann, Weber, Jeffrey S, Whiteside, Theresa L, Wolchok, Jedd D, Zitvogel, Laurence, Zou, Weiping, Kroemer, Guido, Galluzzi, Lorenzo, Vacchelli, Erika, Bravo-San Pedro, José-Manuel, Buqué, Aitziber, Senovilla, Laura, Baracco, Elisa Elena, Bloy, Norma, Castoldi, Francesca, Abastado, Jean-Pierre, Agostinis, Patrizia, Apte, Ron N, Aranda, Fernando, Ayyoub, Maha, Beckhove, Philipp, Blay, Jean-Yves, Bracci, Laura, Caignard, Anne, Castelli, Chiara, Cavallo, Federica, Celis, Estaban, Cerundolo, Vincenzo, Clayton, Aled, Colombo, Mario P, Coussens, Lisa, Dhodapkar, Madhav V, Eggermont, Alexander M, Fearon, Douglas T, Fridman, Wolf H, Fučíková, Jitka, Gabrilovich, Dmitry I, Galon, Jérôme, Garg, Abhishek, Ghiringhelli, François, Giaccone, Giuseppe, Gilboa, Eli, Gnjatic, Sacha, Hoos, Axel, Hosmalin, Anne, Jäger, Dirk, Kalinski, Pawel, Kärre, Klas, Kepp, Oliver, Kiessling, Rolf, Kirkwood, John M, Klein, Eva, Knuth, Alexander, Lewis, Claire E, Liblau, Roland, Lotze, Michael T, Lugli, Enrico, Mach, Jean-Pierre, Mattei, Fabrizio, Mavilio, Domenico, Melero, Ignacio, Melief, Cornelis J, Mittendorf, Elizabeth A, Moretta, Lorenzo, Odunsi, Adekunke, Okada, Hideho, Palucka, Anna Karolina, Peter, Marcus E, Pienta, Kenneth J, Porgador, Angel, Prendergast, George C, Rabinovich, Gabriel A, Restifo, Nicholas P, Rizvi, Naiyer, Sautès-Fridman, Catherine, Schreiber, Hans, Seliger, Barbara, Shiku, Hiroshi, Silva-Santos, Bruno, Smyth, Mark J, Speiser, Daniel E, Spisek, Radek, Srivastava, Pramod K, Talmadge, James E, Tartour, Eric, Van Der Burg, Sjoerd H, Van Den Eynde, Benoît J, Vile, Richard, Wagner, Hermann, Weber, Jeffrey S, Whiteside, Theresa L, Wolchok, Jedd D, Zitvogel, Laurence, Zou, Weiping, and Kroemer, Guido
Abstract: During the past decades, anticancer immunotherapy has evolved from a promising therapeutic option to a robust clinical reality. Many immunotherapeutic regimens are now approved by the US Food and Drug Administration and the European Medicines Agency for use in cancer patients, and many others are being investigated as standalone therapeutic interventions or combined with conventional treatments in clinical studies. Immunotherapies may be subdivided into "passive" and "active" based on their ability to engage the host immune system against cancer. Since the anticancer activity of most passive immunotherapeutics (including tumor-targeting monoclonal antibodies) also relies on the host immune system, this classification does not properly reflect the complexity of the drug-host-tumor interaction. Alternatively, anticancer immunotherapeutics can be classified according to their antigen specificity. While some immunotherapies specifically target one (or a few) defined tumor-associated antigen(s), others operate in a relatively non-specific manner and boost natural or therapy-elicited anticancer immune responses of unknown and often broad specificity. Here, we propose a critical, integrated classification of anticancer immunotherapies and discuss the clinical relevance of these approaches.
Published: 2014

90. Classification of current anticancer immunotherapies

Author: Galluzzi, Lorenzo, primary, Vacchelli, Erika, additional, Pedro, José-Manuel Bravo-San, additional, Buqué, Aitziber, additional, Senovilla, Laura, additional, Baracco, Elisa Elena, additional, Bloy, Norma, additional, Castoldi, Francesca, additional, Abastado, Jean-Pierre, additional, Agostinis, Patrizia, additional, Apte, Ron N., additional, Aranda, Fernando, additional, Ayyoub, Maha, additional, Beckhove, Philipp, additional, Blay, Jean-Yves, additional, Bracci, Laura, additional, Caignard, Anne, additional, Castelli, Chiara, additional, Cavallo, Federica, additional, Celis, Estaban, additional, Cerundolo, Vincenzo, additional, Clayton, Aled, additional, Colombo, Mario P., additional, Coussens, Lisa, additional, Dhodapkar, Madhav V., additional, Eggermont, Alexander M., additional, Fearon, Douglas T., additional, Fridman, Wolf H., additional, Fučíková, Jitka, additional, Gabrilovich, Dmitry I., additional, Galon, Jérôme, additional, Garg, Abhishek, additional, Ghiringhelli, François, additional, Giaccone, Giuseppe, additional, Gilboa, Eli, additional, Gnjatic, Sacha, additional, Hoos, Axel, additional, Hosmalin, Anne, additional, Jäger, Dirk, additional, Kalinski, Pawel, additional, Kärre, Klas, additional, Kepp, Oliver, additional, Kiessling, Rolf, additional, Kirkwood, John M., additional, Klein, Eva, additional, Knuth, Alexander, additional, Lewis, Claire E., additional, Liblau, Roland, additional, Lotze, Michael T., additional, Lugli, Enrico, additional, Mach, Jean-Pierre, additional, Mattei, Fabrizio, additional, Mavilio, Domenico, additional, Melero, Ignacio, additional, Melief, Cornelis J., additional, Mittendorf, Elizabeth A., additional, Moretta, Lorenzo, additional, Odunsi, Adekunke, additional, Okada, Hideho, additional, Palucka, Anna Karolina, additional, Peter, Marcus E., additional, Pienta, Kenneth J., additional, Porgador, Angel, additional, Prendergast, George C., additional, Rabinovich, Gabriel A., additional, Restifo, Nicholas P., additional, Rizvi, Naiyer, additional, Sautès-Fridman, Catherine, additional, Schreiber, Hans, additional, Seliger, Barbara, additional, Shiku, Hiroshi, additional, Silva-Santos, Bruno, additional, Smyth, Mark J., additional, Speiser, Daniel E., additional, Spisek, Radek, additional, Srivastava, Pramod K., additional, Talmadge, James E., additional, Tartour, Eric, additional, Van Der Burg, Sjoerd H., additional, Van Den Eynde, Benoît J., additional, Vile, Richard, additional, Wagner, Hermann, additional, Weber, Jeffrey S., additional, Whiteside, Theresa L., additional, Wolchok, Jedd D., additional, Zitvogel, Laurence, additional, Zou, Weiping, additional, and Kroemer, Guido, additional
Published: 2014
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91. Cancer exosomes trigger mesenchymal stem cell differentiation into pro-angiogenic and pro-invasive myofibroblasts

Author: Chowdhury, Ridwana, primary, Webber, Jason P., additional, Gurney, Mark, additional, Mason, Malcolm D., additional, Tabi, Zsuzsanna, additional, and Clayton, Aled, additional
Published: 2014
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92. EVpedia: a community web portal for extracellular vesicles research

Author: Kim, Dae-Kyum, primary, Lee, Jaewook, additional, Kim, Sae Rom, additional, Choi, Dong-Sic, additional, Yoon, Yae Jin, additional, Kim, Ji Hyun, additional, Go, Gyeongyun, additional, Nhung, Dinh, additional, Hong, Kahye, additional, Jang, Su Chul, additional, Kim, Si-Hyun, additional, Park, Kyong-Su, additional, Kim, Oh Youn, additional, Park, Hyun Taek, additional, Seo, Ji Hye, additional, Aikawa, Elena, additional, Baj-Krzyworzeka, Monika, additional, van Balkom, Bas W. M., additional, Belting, Mattias, additional, Blanc, Lionel, additional, Bond, Vincent, additional, Bongiovanni, Antonella, additional, Borràs, Francesc E., additional, Buée, Luc, additional, Buzás, Edit I., additional, Cheng, Lesley, additional, Clayton, Aled, additional, Cocucci, Emanuele, additional, Dela Cruz, Charles S., additional, Desiderio, Dominic M., additional, Di Vizio, Dolores, additional, Ekström, Karin, additional, Falcon-Perez, Juan M., additional, Gardiner, Chris, additional, Giebel, Bernd, additional, Greening, David W., additional, Gross, Julia Christina, additional, Gupta, Dwijendra, additional, Hendrix, An, additional, Hill, Andrew F., additional, Hill, Michelle M., additional, Nolte-'t Hoen, Esther, additional, Hwang, Do Won, additional, Inal, Jameel, additional, Jagannadham, Medicharla V., additional, Jayachandran, Muthuvel, additional, Jee, Young-Koo, additional, Jørgensen, Malene, additional, Kim, Kwang Pyo, additional, Kim, Yoon-Keun, additional, Kislinger, Thomas, additional, Lässer, Cecilia, additional, Lee, Dong Soo, additional, Lee, Hakmo, additional, van Leeuwen, Johannes, additional, Lener, Thomas, additional, Liu, Ming-Lin, additional, Lötvall, Jan, additional, Marcilla, Antonio, additional, Mathivanan, Suresh, additional, Möller, Andreas, additional, Morhayim, Jess, additional, Mullier, François, additional, Nazarenko, Irina, additional, Nieuwland, Rienk, additional, Nunes, Diana N., additional, Pang, Ken, additional, Park, Jaesung, additional, Patel, Tushar, additional, Pocsfalvi, Gabriella, additional, del Portillo, Hernando, additional, Putz, Ulrich, additional, Ramirez, Marcel I., additional, Rodrigues, Marcio L., additional, Roh, Tae-Young, additional, Royo, Felix, additional, Sahoo, Susmita, additional, Schiffelers, Raymond, additional, Sharma, Shivani, additional, Siljander, Pia, additional, Simpson, Richard J., additional, Soekmadji, Carolina, additional, Stahl, Philip, additional, Stensballe, Allan, additional, Stępień, Ewa, additional, Tahara, Hidetoshi, additional, Trummer, Arne, additional, Valadi, Hadi, additional, Vella, Laura J., additional, Wai, Sun Nyunt, additional, Witwer, Kenneth, additional, Yáñez-Mó, María, additional, Youn, Hyewon, additional, Zeidler, Reinhard, additional, and Gho, Yong Song, additional
Published: 2014
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93. Tumor stroma-derived factors skew monocyte to dendritic cell differentiation toward a suppressive CD14+PD-L1+phenotype in prostate cancer

Author: Spary, Lisa K, primary, Salimu, Josephine, additional, Webber, Jason P, additional, Clayton, Aled, additional, Mason, Malcolm D, additional, and Tabi, Zsuzsanna, additional
Published: 2014
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94. Enhancement of T Cell Responses as a Result of Synergy between Lower Doses of Radiation and T Cell Stimulation

Author: Spary, Lisa K., primary, Al-Taei, Saly, additional, Salimu, Josephine, additional, Cook, Alexander D., additional, Ager, Ann, additional, Watson, H. Angharad, additional, Clayton, Aled, additional, Staffurth, John, additional, Mason, Malcolm D., additional, and Tabi, Zsuzsanna, additional
Published: 2014
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95. Proteomics Analysis of Cancer Exosomes Using a Novel Modified Aptamer-based Array (SOMAscanTM) Platform

Author: Webber, Jason, primary, Stone, Timothy C., additional, Katilius, Evaldas, additional, Smith, Breanna C., additional, Gordon, Bridget, additional, Mason, Malcolm D., additional, Tabi, Zsuzsanna, additional, Brewis, Ian A., additional, and Clayton, Aled, additional
Published: 2014
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96. Young women with polycystic ovary syndrome have increased concentrations of circulating annexin V-positive microparticles derived predominantly from platelets

Author: Willis, Gareth, primary, Connolly, Katherine, additional, Ladell, Kristin, additional, Miners, Kelly, additional, Price, David, additional, Guschina, Irina, additional, Clayton, Aled, additional, James, Philip, additional, and Rees, Aled, additional
Published: 2014
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97. Cross-presentation of the oncofoetal tumor antigen 5T4 from irradiated prostate cancer cells - a key role for Hsp70

Author: Salimu, Josephine C, primary, Spary, Lisa K, additional, Al-Taei, Saly, additional, Clayton, Aled, additional, Mason, Malcolm, additional, Staffurth, John, additional, and Tabi, Zsuzsanna, additional
Published: 2014
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98. Vesiclepedia : a compendium for extracellular vesicles with continuous community annotation

Author: Kalra, Hina, Simpson, Richard J, Ji, Hong, Aikawa, Elena, Altevogt, Peter, Askenase, Philip, Bond, Vincent C, Borràs, Francesc E, Breakefield, Xandra, Budnik, Vivian, Buzas, Edit, Camussi, Giovanni, Clayton, Aled, Cocucci, Emanuele, Falcon-Perez, Juan M, Gabrielsson, Susanne, Gho, Yong Song, Gupta, Dwijendra, Harsha, HC, Hendrix, An, Hill, Andrew F, Inal, Jameel M, Jenster, Guido, Krämer-Albers, Eva-Maria, Lim, Sai Kiang, Llorente, Alicia, Lötvall, Jan, Marcilla, Antonio, Mincheva-Nilsson, Lucia, Nazarenko, Irina, Nieuwland, Rienk, Nolte-'t Hoen, Esther NM, Pandey, Akhilesh, Patel, Tushar, Piper, Melissa G, Pluchino, Stefano, Prasad, TS Keshava, Rajendran, Lawrence, Raposo, Graca, Record, Michel, Reid, Gavin E, Sánchez-Madrid, Francisco, Schiffelers, Raymond M, Siljander, Pia, Stensballe, Allan, Stoorvogel, Willem, Taylor, Douglas, Thery, Clotilde, Valadi, Hadi, van Balkom, Bas WM, Vázquez, Jesús, Vidal, Michel, Wauben, Marca HM, Yáñez-Mó, María, Zoeller, Margot, Mathivanan, Suresh, Kalra, Hina, Simpson, Richard J, Ji, Hong, Aikawa, Elena, Altevogt, Peter, Askenase, Philip, Bond, Vincent C, Borràs, Francesc E, Breakefield, Xandra, Budnik, Vivian, Buzas, Edit, Camussi, Giovanni, Clayton, Aled, Cocucci, Emanuele, Falcon-Perez, Juan M, Gabrielsson, Susanne, Gho, Yong Song, Gupta, Dwijendra, Harsha, HC, Hendrix, An, Hill, Andrew F, Inal, Jameel M, Jenster, Guido, Krämer-Albers, Eva-Maria, Lim, Sai Kiang, Llorente, Alicia, Lötvall, Jan, Marcilla, Antonio, Mincheva-Nilsson, Lucia, Nazarenko, Irina, Nieuwland, Rienk, Nolte-'t Hoen, Esther NM, Pandey, Akhilesh, Patel, Tushar, Piper, Melissa G, Pluchino, Stefano, Prasad, TS Keshava, Rajendran, Lawrence, Raposo, Graca, Record, Michel, Reid, Gavin E, Sánchez-Madrid, Francisco, Schiffelers, Raymond M, Siljander, Pia, Stensballe, Allan, Stoorvogel, Willem, Taylor, Douglas, Thery, Clotilde, Valadi, Hadi, van Balkom, Bas WM, Vázquez, Jesús, Vidal, Michel, Wauben, Marca HM, Yáñez-Mó, María, Zoeller, Margot, and Mathivanan, Suresh
Abstract: Extracellular vesicles (EVs) are membraneous vesicles released by a variety of cells into their microenvironment. Recent studies have elucidated the role of EVs in intercellular communication, pathogenesis, drug, vaccine and gene-vector delivery, and as possible reservoirs of biomarkers. These findings have generated immense interest, along with an exponential increase in molecular data pertaining to EVs. Here, we describe Vesiclepedia, a manually curated compendium of molecular data (lipid, RNA, and protein) identified in different classes of EVs from more than 300 independent studies published over the past several years. Even though databases are indispensable resources for the scientific community, recent studies have shown that more than 50% of the databases are not regularly updated. In addition, more than 20% of the database links are inactive. To prevent such database and link decay, we have initiated a continuous community annotation project with the active involvement of EV researchers. The EV research community can set a gold standard in data sharing with Vesiclepedia, which could evolve as a primary resource for the field.
Published: 2012
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99. Transforming Growth Factor-β1 (TGF-β1)-stimulated Fibroblast to Myofibroblast Differentiation Is Mediated by Hyaluronan (HA)-facilitated Epidermal Growth Factor Receptor (EGFR) and CD44 Co-localization in Lipid Rafts

Author: Midgley, Adam C., primary, Rogers, Mathew, additional, Hallett, Maurice B., additional, Clayton, Aled, additional, Bowen, Timothy, additional, Phillips, Aled O., additional, and Steadman, Robert, additional
Published: 2013
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100. How pure are your vesicles?

Author: Webber, Jason, primary and Clayton, Aled, additional
Published: 2013
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