51. Protection against Trichinella spiralis in BALB/c mice via oral administration of recombinant Lactobacillus plantarum expressing murine interleukin-4
- Author
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Wen-Tao Yang, Chun-Feng Wang, Yuanhuan Kang, Gui-Lian Yang, Chun-Wei Shi, Jian-Zhong Wang, Dan Wang, Yanlong Jiang, Qing-long Gong, Hai-Bin Huang, and Quan Zhao
- Subjects
medicine.medical_treatment ,Trichinella spiralis ,Administration, Oral ,Microbiology ,law.invention ,BALB/c ,Mice ,Immune system ,law ,medicine ,Animals ,Interleukin 4 ,Mice, Inbred BALB C ,General Veterinary ,biology ,Immunogenicity ,Probiotics ,Trichinellosis ,General Medicine ,biology.organism_classification ,Cytokine ,Recombinant DNA ,Parasitology ,Female ,Interleukin-4 ,Microorganisms, Genetically-Modified ,Lactobacillus plantarum - Abstract
Interleukin-4 (IL-4), an immunomodulatory cytokine derived from activated T lymphocytes were shown to regulate Th2-type immune responses. It plays an important role in anti-parasitic infections. In this study, a recombinant plasmid was designed using murine IL-4 co-expressed with pgsA anchor system of Lactobacillus plantarum NC8 and was delivered by live Lactobacillus plantarum NC8, which exhibited an enhanced immunogenicity in protection of BALB/c mice from infection with Trichinella spiralis. The results showed that the levels of serum IgG1 and mucosal secretory IgA (sIgA) were both increased significantly in mice orally inoculated with NC8-pSIP409-pgsA-mIL-4, and the Th2 phenotype immune response was up-regulated. A 29.9 % reduction in adult worm burden at 7 days post-infection (dpi) and 83.3 % reduction in muscle larvae burden at 28 dpi were observed in immune-stimulated mice with NC8-pSIP409-pgsA-mIL-4. Moreover, weight loss and pathological changes were also improved in mice of NC8-pSIP409-pgsA-mIL-4 group. Taken together, it suggests that NC8-pSIP409-pgsA-mIL-4 could improve the intestinal mucosal immunity and promoted the elimination of the adult worm in Trichinella-infected mice. This study laid the foundation for the development of a novel vaccines against Trichinellosis.
- Published
- 2020