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Iridoid glycosides and flavanoids are two main effective components of Lamiophlomis rotata (Benth.) kudo. However, there is no method for simultaneous analysis of iridoid glycosides and flavanoids in L. rotata and its pharmaceutical preparations. A simple and rapid capillary zone electrophoresis (CZE) method was developed and validated for simultaneous determination of two iridoid glycosides (8-O-acetylshanzhiside methylester and 8-deoxyshanzhiside) and three flavanoids (apigenin, quercetin and luteolin) in L. rotata. Operational variables, such as the voltage, buffer concentration and pH were optimized, the final optimum separation condition was 10 mM sodium tetraborate-20 mM NaH2PO4 (pH 8.5)-15% (v/v) methanol, 238 nm UV detection, 18 kV applied voltage. The linearity and the recovery of the proposed method were very satisfactory (correlation coefficients were 0.9994-0.9998 and the recoveries were 94.5-108.8% for the analytes) and the method allowed analytes in real samples to be determined within 9 min. The proposed CZE method can be used for quality control of iridoid glycosides and flavanoids in L. rotata and its herbal preparation. Copyright © 2011 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Abstract: A capillary zone electrophoresis method using only 1-alkyl-3-methylimidazolium-based ionic liquids as background electrolyte for the simultaneous determination of five anthraquinone derivatives including aloe-emodin, emodin, chrysophanol, physcion and rhein in Rhubarb species was described. Ion association constants, K ass, between anthraquinone anions and imidazolium cations were determined by analyzing the electrophoretic mobility change of anthraquinone anions using a non-linear least-squares method and factors contributing to ion associability were systematically clarified. For method optimization, several parameters such as ionic liquids concentration, background electrolyte pH and applied voltage, on the separation were evaluated and the optimum conditions were obtained as follows: 90mM 1-butyl-3-methylimidazolium tetrafluoroborate (pH 11.0) with an applied voltage of 20kV. Under these conditions, the method has been successfully applied to the determination of anthraquinones in extracts of two kinds of Rhubarb plants (R. palmatum and R. hotaoense) within 12min. The method proposed herein was shown to be much simpler than the previously reported methods. [Copyright &y& Elsevier]

Co 3 O 4 nanoparticles-graphene oxide (Co 3 O 4 NPs-GO) nanocomposites with good solubility were successfully synthesized and well characterized. As a nanocatalyst with oxidase-mimicking activity, the nanocomposites can catalyze the oxidation of 3',5,5'-tetramethylbenzidine (TMB) with high efficiency. The catalyzing reaction was rapid and no extra H 2 O 2 was needed compared with other similar TMB oxidation reactions. The catalyzing reaction mechanism of the system was investigated in detail. And it was demonstrated that the oxygen involved in the reaction came from the oxygen absorbed on the nanocomposites which oxidized TMB. Based on this reaction, a colorimetric system for vitamin C detection in vegetable and fruits was established. Under the optimum conditions, the detection can be achieved within 10 min and a linear relationship in concentration range of 2.5 × 10 -6 to 1.8 × 10 -5  M and 3.4 × 10 -5 to 1.7 × 10 -4  M was obtained with a detection limit of 7.0 × 10 -7  M. The colorimetric system exhibited good selectivity, sensitivity and satisfactory recoveries ranged from 93.1% to 101.1%., (Copyright © 2018 Elsevier Inc. All rights reserved.)

This work describes a "switch-on" fluorescence approach for sensing of ascorbic acid (AA) in food samples. In the present method, the fluorescence intensity (FL) of carbon quantum dots (CQDs) was first quenched by addition of MnO2 nanosheets through an inner filter effect to form a CQDs-MnO2 probe. When reductive AA was introduced into the quenched CQDs solution, the added MnO2 was destroyed due to the redox reaction between AA and MnO2 nanosheets, and the FL of the system was recovered. Under the optimal conditions, the limit of detection for AA was 42 nM, with a wide concentration linear range of 0.18-90 μM. Furthermore, the as-fabricated fluorescent sensing system was successfully applied to the analysis of AA in fresh fruits, vegetables, and commercial fruit juices samples with satisfactory results.