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Additional file 1: Figure S1. TEM image of nude MSN without PEGylation and Fcfragments. Figure S2. Representative size distribution profiles of MSN-SH,MSN-COOH, MSN-Fc, and NISA. Figure S3. Surfaceelement components of the nanoparticles were detected using X-ray photoelectronspectroscopy (XPS) assay. The changes of surface N1s confirmed the sequentialmodification and loading of the functional molecules and IgG3 Fc. FigureS4. Fc conjugation efficiency along with increased feeding amount. Data areexpressed as means �� SD (n = 3). Figure S5. Examination of the left IgG3 Fc in the supernatantof MSN-Fc using SDS-PAGE. Figure S6. IgG3 Fc retention on NISA in PBScontaining 10% FBS at 37 ���. Data are expressed as means �� SD (n = 3). FigureS7. Colloid stability of NISA in PBS at 4 ��� for 7 days. Data are expressedas means �� SD (n = 3). Figure S8. Fc�� receptors on the cell membrane ofmacrophages (RAW264.7) and dendritic cells (DC2.4). Fc�� receptors wasidentified using anti-CD64/Fc��R rabbit polyclonal antibody (1:500, SinoBiological, Beijing, China) and Alexa Fluor 488-labeled goat anti-rabbit IgG secondaryantibody (1:1000, Abcam, Shanghai, China) for RAW264.7 or Alexa Fluor 647-labeledgoat anti-rabbit IgG secondary antibody (1:1000, ThermoFisher, Shanghai, China)for DC2.4. Figure S9. Examination of ERK activation in the cells treatedwith free Fc or MSN-Fc. (A) Western blot assay of p-ERK expression inRAW264.7 and DC2.4 cells. Statistical assay (B, C) of p-ERK expressionin panel A. Data are expressed as means �� SD (n = 3). Figure S10.MSN-Fc treatment induced the formation of more pseudopodiums in RAW264.7 andDC2.4 cells. The cell pseudopodiums were observed through the immunofluorescentstaining of F-actin using acti-stain 670 phalloidin. Notedthat TNF-�� (green color) in the cells was stained with rabbitanti-TNF-�� antibody (Abcam, Hong Kong),and Alexa Fluor 546 donkey anti-rabbit IgG (Thermo Fisher, Waltham)and observed at Ex 556 nm and Em 573 nm. FigureS11. Blood clearance curve of free Fc (iFluor 647 labeled). Thestandard curve between iFluor 647 fluorescence intensity and corresponding Fcconcentration was used to determine the Fc content in blood. Data are expressedas means �� SD (n = 5). Figure S12. Quantification of nanoparticle content in tumors.The dose of MSN-Fc (A) and NISA (B) in the tumors was determinedusing the established standard curve between the iFluor 647 fluorescenceintensity and the corresponding nanoparticle (MSN-Fc or NISA) concentrations.#1, #2, and #3 were three separate tumor samples. 6 h after injection, 1.2% ofthe injected MSN-Fc was obtained at the tumor site. In contrast, dramaticallyincreased proportion of the injected NISA (2.2%) was found in the tumor. FigureS13. Individual tumor growth curves of the mice in each group. Figure S14.The antitumor effect of Fc, Fc + fMLP and MSN-Fc in orthotopic 4T1-bearingmice. (A) Tumor growth curve (n = 5, mean �� SEM).S. a, NISA versus MSN-Fc. b, NISA versus Saline, Fc, and Fc + fMLP. c, NISA + fMLPversus all other groups. (B) Mouse body weight (n = 5, mean �� SD). Thegroups of saline, NISA, and NISA + fMLP indicated with dotted blue lines werealso displayed in Fig. 5 in the main text. Figure S15. On day 9 (24 hafter the final injection), 3 mice from each group were sacrificed, and themajor organs (heart, liver, spleen, lung, and kidney) of the mice were removedand processed for paraffin sections and histopathological examination (H&Estaining). Figure S16. Macrophagepolarization analysis. (A) Macrophages are defined as F4/80+cell. M1-like cells are F4/80+CD11c+CD206���,whereas M2-like cells are F4/80+CD11c���CD206+cells. (B) Statistical assay of the M1- and M2-like cell proportion intotal macrophages. Data are expressed as mean �� SD. n = 3. ***p

Since MTB has been deemed as the business model of 21st century but its long-term sustainability depends on level of consumer satisfaction with its applicability in their everyday life. Therefore, current research aims to highlight factors which could shape consumers’ satisfaction and further trigger their intuition to recommend it in their circle of influence. Unified theory of acceptance and use of technology 2 (utaut2) was modified and extended to fit the purpose. Data were collected from prevailing MTB users and analyzed in PLS-SEM. Results reveal that utaut2 factors signify their effects on consumer satisfaction which further trigger their instinct to recommend MTB. Current research modified utaut2 with mediating role of consumer satisfaction with MTB which further stimulates their instinct to recommend it. The modified framework adds value to the theoretical horizon of utaut2 and further extends its applicability in a novel setting like MTB. The study offers insightful implications to MTB companies for effective design and implementation of future strategies. Moreover, limitations and suggestions for future researchers are also discussed.

Additional file 3: Figure S1. Identification of lncRNA-HGBC and its noncoding nature analysis. Figure S2. LncRNA-HGBC promotes GBC cell proliferation and tumor growth. Figure S3. LncRNA-HGBC promotes the invasive capacity of GBC cells. Figure S4. LncRNA-HGBC did not influence HuR expression. Figure S5. Effects of miR-502-3p on lncRNA-HGBC expression. Figure S6. SET is a direct target of miR-502-3p in GBC cells. Figure S7. miR-502-3p inhibits GBC cell proliferation and invasion. Figure S8. Knockdown of SET inhibits GBC cell proliferation and invasion. Figure S9. HuR is upregulated in GBC tissues.

Additional file 1: Figure S1. AEP-triggered fluorescence enhancement of AuNPs-Cy5.5-A&C. In pH 5.0 HEPES buffer, AuNPs-Cy5.5-A&C was incubated without or with AEP (1 mg ml−1) for 12 h. 20 μM Atorvastatin (Ato) was used to inhibit AEP. AuNPs-Cy5.5-AK or AuNPs-Cy5.5-CABT alone was also incubated in the same buffer. Fluorescent intensity was measured by a microplate reader. **P 0.05, Student’s t-test, n = 8 mice per group. (E–F) The latency to the escape platform and swimming speed of 8-month old mice over the 5-day acquisition training. *P

Additional file 2. Supplementary Methods.