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55. Evolution of the target design for the MJ laser

Author

HOLSTEIN, P.A., primary, CHALAND, F., additional, CHARPIN, C., additional, DUFOUR, J.M., additional, DUMONT, H., additional, GIORLA, J., additional, HALLO, L., additional, LAFFITE, S., additional, MALINIE, G., additional, SAILLARD, Y., additional, SCHURTZ, G., additional, VANDENBOOMGAERDE, M., additional, and WAGON, F., additional

Published
1999
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58. Desmoid tumour of the breast.

Author

Terrier, J P, primary, Garcia, S, additional, Bessenay, F, additional, Sappa, P, additional, Andrac-Meyer, L, additional, Bonnier, P, additional, and Charpin, C, additional

Published
1998
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71. Reappraisal of Breast Hamartomas

Author

Charpin, C., primary, Mathoulin, M.P., additional, Andrac, L., additional, Barberis, J., additional, Boulat, J., additional, Sarradour, B., additional, Bonnier, P., additional, and Piana, L., additional

Published
1994
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74. Non Palpable Breast Carcinomas

Author

Charpin, C., primary, Bonnier, P., additional, Khouzami, A., additional, Andrac, L., additional, Habib, M., additional, Vacheret, H., additional, Lavaut, M.-N., additional, and Piana, L., additional

Published
1993
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80. Histology of Expanded Skin

Author

Andrac, L., primary, Aubert, J-P., additional, Vacheret, H., additional, Habib, M-C., additional, Charpin, C., additional, and Magalon, G., additional

Published
1991
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84. Clinicopathologic Growth Factors in Vestibular Schwannomas: a Morphological and Immunohistochemical Study of 69 Tumours.

Author

Labit-Bouvier, C., Crebassa, B., Bouvier, C., Andrac-Meyer, L., Magnan, J., and Charpin, C.

Subjects
ACOUSTIC neuroma, PATHOLOGY
Abstract

Tumour growth of vestibular schwannomas is still difficult to predict. The aim of our study was to determine whether any defined histopathological feature was correlated with the clinical course. We did a retrospective study with 69 paraffin-embedded tumours to establish whether the number of vessels, blood cells extravasation or degree of inflammation, all semi-quantitatively assessed, could be indicative of potential of growth. An immunohistochemical study was also performed with an endothelial marker CD34, the leukocyte common antigen CD45 and the estrogen and progesterone hormone receptors. All these parameters were correlated with patient's age, duration of symptoms (d), with a clinical growth index (CLI = tumour size/d). No clinical parameters proved to be predictive of tumour growth. Tumour size was significantly (p = 0.01) related to the number of vessels and we found a significant relationship between the clinical growth index (CLI) and total number of vessels, especially when duration of symptoms lasted less than 1 year (p < 0.001). However, we found no relationship between duration of symptoms or CLI and CD34 index. The degree of inflammation was significantly correlated (p = 0.007) with duration of symptoms when it lasted more than 1 year. The CD45 index and the semi-quantitative evaluation of the inflammation were well correlated (p = 0.001). No estrogen receptors antigenic site was detected and only seven tumours expressed progesterone receptor in a few cells without any significant clinical value. These results suggest that vessel density is determinant for sporadic acoustic neuroma growth especially for a short clinical course. [ABSTRACT FROM AUTHOR]

Published
2000
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86. Effects of castration and testosterone on Fel d I production by sebaceous glands of male cats: II--morphometric assessment.

Author

Charpin, C., Zielonka, T. M., Charpin, D., Ansaldi, J. -L., Allasia, C., and Vervloet, D.

Subjects
*TESTOSTERONE, *ANDROGENS, *CYTOPLASM, *CELL membranes, *EXOCRINE secretions, *CASTRATION
Abstract

A morphometric study of cat sebaceous glands was performed to evaluate the effects of castration and testosterone treatments. Skin biopsies were taken in six cats before castration, after castration and after the testosterone injections administered after castration (total number of biopsies: 18). Ninety 8/im thick sections of each biopsy were assessed for image analysis processing (SAMBA 2005, ALCATEL TITN). The variations in glands and cells size were evaluated on digitized microscopic images by morphometric parameters included in the SAMBA software package. An original software was developed for the analysis of the spacial gland structure. The best morphometric parameters were selected in a first step of the study, and included the nuclear surface (NS), the cell surface (CS) and the nuclear/cellular surface ratio (N/C). These three parameters were then compared in each group of samples for the six cats. It was shown that after castration the N/C (21%) significantly increased compared with prior to castration (12.6%). This 59.8% increase was mainly due to cell cytoplasm shrinking reflecting a decrease of the cell activity. The testosterone administered after castration produced a reverse effect with a N/C ratio back to normal (11.4%) and a significant cell cytoplasm and gland enlargement as shown by the three dimension constructions. This morphometric data correlated with the measurement of sebum and Fel d I productions. The negative effects of castration and the positive effects of testosterone on the sebaceous cells and glands volume favour the hypothesis that cat sebaceous cells are subject to hormonal control this is also likely to apply to the Fel d I production. [ABSTRACT FROM AUTHOR]

Published
1994
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88. Immunodetection in fine-needle aspirates and multiparametric (SAMBA) image analysis. Receptors (monoclonal antiestrogen and antiprogesterone) and growth fraction (monoclonal Ki67) evaluation in breast carcinomas.

Author

Charpin, Colette, Andrac, Lucile, Habib, Marie-Christine, Vacheret, Helene, Xerri, Luc, Devictor, Benedicte, Noelle Lavaut, Marie, Toga, Maurice, Charpin, C, Andrac, L, Habib, M C, Vacheret, H, Xerri, L, Devictor, B, Lavaut, M N, and Toga, M

Published
1989
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89. Localization of lactoferrin and nonspecific cross-reacting antigen in human breast carcinomas. An immunohistochemical study using the avidin-biotin-peroxidase complex method.

Author

Charpin, Colette, Lachard, Alain, Pourreau-Schneider, Natalie, Jacquemier, Jocelyne, Lavaut, Marie Noëlle, Andonian, Claudie, Martin, Pierre Marie, Toga, Maurice, Charpin, C, Lachard, A, Pourreau-Schneider, N, Jacquemier, J, Lavaut, M N, Andonian, C, Martin, P M, and Toga, M

Published
1985
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91. Laminin biosynthesis in the extracellular matrix-producing cell line PFHR9 studied with monoclonal and polyclonal antibodies

Author

Lissitzky, J C S, Charpin, C, Bignon, C, Bouzon, M, Kopp, F, Delori, P, and Martin, P M

Abstract

The biosynthesis of the basement-membrane glycoprotein laminin in the mouse teratocarcinoma cell line PFHR9 was studied by immunoelectron microscopy and pulse-chase experiments using monoclonal and polyclonal antibodies. By immunoelectron microscopy, most of the protein was found to be aggregated on the outer cell surface. Cytoplasmic stainings were rare and were located next to the intracellular side of the plasma membrane. Sequential immunoprecipitations of cell extracts with a monoclonal antibody (4C12) sensitive to the laminin native conformation and with a polyclonal antibody enables laminin, the B1 subunit and a 410 kDa molecule to be distinguished. Most of the laminin is of the A(B1B2) type, and the 410 kDa molecule appears to be a B1B2 heterodimer. The assembly of laminin from subunits is completed in less than 1 h, and B chains are incorporated via the formation of the B heterodimers. The B2 and A chains are not found as free forms, so their levels appear to be the rate-limiting factors for the assembly of the dimers and laminin respectively. The formation of an uncross-linked A(B1B2) complex as a short-lived intermediate in the biosynthetic process is possible. Together with immunoelectron microscopy, the present study suggests that the protein is rapidly exported after assembly to accumulate on the outer side of the cell membrane. The biosynthesis of laminin in the PFHR9 cell line appears to be similar to that in other matrix-producing cell lines.

Published
1988
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92. VLA2integrin expression in breast carcinomas evaluated by automated and quantitative immunohistochemistry

Author

Charpin, C, Garcia, S, Bergeret, D, Andrac, L, Horschowski, N, Choux, R, and Lavaut, MN

Abstract

VLA2 is thought to be involved in the metastatic process in malignant tumours, in particular in carcinomatous cell adhesion to vessel basement membrane. VLA2 expression was immunohistochemically investigated in 204 breast carcinomas. Frozen tissue sections were probed with monoclonal anti-VLA2 using automated (Ventana ES 320 System) and quantitative (SAMBA 2005 image processor) immunoperoxidase. A positive anti-VLA2 immunoreaction was observed in 48 tumours (23.5%), within epithelial carcinomatous cells. The VLA2-positive surface in tumours varied from 3% to 20% (mean 8.75, S.D. 7.17) and was correlated with histoprognostic indicators and tumour expression of various antigens detected using the same method as that for VLA2. The results show that VLA2 immunoexpression was independent of the tumour size, grade, type and aneuploidy, and of the nodal status. VLA2 significantly correlated with ELAM, VCAM, VLA3 and P-glycoprotein (P-gp) (P < 0.01) and inversely correlated with cathepsin D (P < 0.001), but was independent of Ki67/MIB1, p53, bcl-2, c-erbB-2, E cadherin, CD44v, CD31, oestrogen and progesterone receptors' (ER, PR) antigenic sites and pS2. The exact role, if any, of VLA2 in tumour cell dissemination remains to be elucidated and the clinical relevance of VLA2 immunodetection in breast carcinomas requires further investigation of the correlation between VLA2 immunocytochemical expression and patients' outcome and response to chemotherapy.

Published
1998
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93. Mutations at BRCA1: The medullary breast carcinoma revisited

Author

Eisinger, F., Jacquemier, J., Charpin, C., Stoppa-Lyonnet, D., Brigitte Bressac-de Paillerets, Peyrat, J. P., Longy, M., Guinebretiere, J. M., Sauvan, R., Noguchi, T., Birnbaum, D., and Sobol, H.

Subjects
Family Health, BRCA1 Protein, Carcinoma, Medullary, DNA Mutational Analysis, Mutation, Humans, Breast Neoplasms, Female
Abstract

BRCA1-associated breast cancers (BRCA1-BCs) frequently harbor a high histoprognostic grade, p53 alterations, and estrogen receptor negativity. Although these parameters predict a poor outlook, the overall survival in BRCA1-BCs is equivalent to or even better than that in sporadic cases. These features are reminiscent of what is observed for breast carcinoma of the medullary type, a high-grade tumor with a particular favorable course. To explore a possible relationship between this phenotype and BRCA1 mutations, we first compared 32 BRCA1-BCs and 200 consecutive cases of breast cancer without familial history for the prevalence of typical medullary breast carcinoma (TMC) using the criteria given by Ridolfi et al. [R. Ridolfi et al, Cancer (Phila.), 40: 1365-1385, 1977]. Second, we searched for BRCA1 mutations in a set of 18 cases of TMC, using denaturing gradient gel electrophoresis and Cleavase fragment length polymorphism scanning. Six of 32 (19%) BRCA1-BCs were of the TMC type, compared to 0 of 200 controls (P0.0001). Among the 18 TMCs, 2 BRCA1 nonsense mutations were found. This corresponds to almost 7 times the contribution of BRCA1 mutations in the general population. Two additional missense mutations were identified. Together, these results suggest that, although TMC and BRCA1-BCs are not strictly coincidental, an important connection between the two populations does exist.

100. Hypoxia inducible factor 1alpha gene (HIF-1alpha) splice variants: potential prognostic biomarkers in breast cancer.

Author

Dales JP, Beaufils N, Silvy M, Picard C, Pauly V, Pradel V, Formisano-Tréziny C, Bonnier P, Giusiano S, Charpin C, Gabert J, Dales, Jean-Philippe, Beaufils, Nathalie, Silvy, Monique, Picard, Christophe, Pauly, Vanessa, Pradel, Vincent, Formisano-Tréziny, Christine, Bonnier, Pascal, and Giusiano, Sophie

Abstract

Background: Hypoxia-inducible factor 1 (HIF-1) is a master transcriptional regulator of genes regulating oxygen homeostasis. The HIF-1 protein is composed of two HIF-1alpha and HIF-1beta/aryl hydrocarbon receptor nuclear translocator (ARNT) subunits. The prognostic relevance of HIF-1alpha protein overexpression has been shown in breast cancer. The impact of HIF-1alpha alternative splice variant expression on breast cancer prognosis in terms of metastasis risk is not well known.Methods: Using real-time quantitative reverse transcription PCR assays, we measured mRNA concentrations of total HIF-1alpha and 4 variants in breast tissue specimens in a series of 29 normal tissues or benign lesions (normal/benign) and 53 primary carcinomas. In breast cancers HIF-1alpha splice variant levels were compared to clinicopathological parameters including tumour microvessel density and metastasis-free survival.Results: HIF-1alpha isoforms containing a three base pairs TAG insertion between exon 1 and exon 2 (designated HIF-1alphaTAG) and HIF-1alpha736 mRNAs were found expressed at higher levels in oestrogen receptor (OR)-negative carcinomas compared to normal/benign tissues (P = 0.009 and P = 0.004 respectively). In breast carcinoma specimens, lymph node status was significantly associated with HIF-1alphaTAG mRNA levels (P = 0.037). Significant statistical association was found between tumour grade and HIF-1alphaTAG (P = 0.048), and total HIF-1alpha (P = 0.048) mRNA levels. HIF-1alphaTAG mRNA levels were also inversely correlated with both oestrogen and progesterone receptor status (P = 0.005 and P = 0.033 respectively). Univariate analysis showed that high HIF-1alphaTAG mRNA levels correlated with shortened metastasis free survival (P = 0.01).Conclusions: Our results show for the first time that mRNA expression of a HIF-1alphaTAG splice variant reflects a stage of breast cancer progression and is associated with a worse prognosis.See commentary: http://www.biomedcentral.com/1741-7015/8/45. [ABSTRACT FROM AUTHOR]

Published
2010
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