Search

Your search keyword '"Chaplin, T"' showing total 312 results
Start Over Author "Chaplin, T"
312 results on '"Chaplin, T"'

57. The MLL fusion partner AF10 binds GAS41, a protein that interacts with the human SWI/SNF complex.

Author

Debernardi, S., Bassini, A., Jones, L.K., Chaplin, T., Linder, B., Bruijn, D.R.H. de, Meese, E., Young, B.D., Debernardi, S., Bassini, A., Jones, L.K., Chaplin, T., Linder, B., Bruijn, D.R.H. de, Meese, E., and Young, B.D.

Abstract

Item does not contain fulltext, The AF10 gene encodes a putative transcription factor containing an N-terminal LAP/PHD zinc finger motif, a functional nuclear localization signal, an AT-hook domain, and a leucine zipper toward the C-terminus. AF10 is involved in 2 distinct chromosomal translocations associated with hematologic malignancy. The chimeric fusion proteins MLL/AF10 and CALM/AF10, resulting from the t(10;11)(p12;q23) and the t(10;11)(p12;q14), respectively, consistently retain the leucine zipper motif of AF10. This part of the C-terminal region was used as bait in a yeast 2 hybrid screening of a testis complementary DNA library. The leucine zipper interacted with GAS41, a protein previously identified as the product of an amplified gene in a glioblastoma. GAS41 shows significant homology to the Saccharomyces cerevisiae protein ANC1 and to the human MLL fusion partners AF9 and ENL. The interaction was confirmed in vivo. Furthermore, the study showed by coimmunoprecipitation that GAS41 interacts with INI1 (Integrase Interactor 1) and that INI1 was present in the AF10 immunoprecipitate. INI1 is the human homologue of the yeast SNF5 protein, a component of the SWI/SNF complex, which acts to remodel chromatin and to modulate transcription. The retention of the leucine zipper in the MLL and CALM fusions suggests that a key feature of these chimeric proteins may be their ability to interfere in normal gene regulation through interaction with the adenosine triphosphate-dependent chromatinremodeling complexes.

Published
2002

69. CHANNEL TUNNEL PART 2: TERMINALS. DISCUSSION OF PAPERS 10122-8.

Author

CHAPLIN, T K, primary, TABERNER, J P, additional, BLAIR FISH, P M, additional, FISH, P M B, additional, NAPTHINE, R, additional, SAXON, R G, additional, GRAHAM, M D, additional, BAYFIELD, R W, additional, MONK, R B, additional, GRANT, J G, additional, LAMBERT, R C, additional, and SMART, R, additional

Published
1995
Full Text
View/download PDF

77. The characterisation of the lymphoma cell line U937, using comparative genomic hybridisation and multi-plex FISH.

Author

Strefford, J. C., Foot, N. J., Chaplin, T., Neat, M. J., Oliver, R. T. D., Young, B. D., and Jones, L. K.

Subjects
CELL lines, LYMPHOMAS, MYELOID leukemia, CYTOGENETICS, DROSOPHILA, KARYOTYPES
Abstract

The cell line U937, which has been used extensively for studies of myeloid differentiation, bears the t(10;11)(p13;q14) translocation which results in a fusion between the MLLT10 (myeloid/lymphoid or mixed-lineage leukemia [trithorax, Drosophila, homolog]; translocated to 10; alias AF10) gene and the Ap-3-like clathrin assembly protein, PICALM (Clathrin assembly lymphoid myeloid leukaemia). Apart from this translocation, very little is known about the other genetic alterations in this cell line that may represent significant events in disease progression. In this study, conventional G-banding, CGH and M-FISH have been used to characterise fully all of the cytogenetic alterations present in the U937 cell line. M-FISH analysis confirmed the presence of the t(10;11) and an apparently normal copy of both chromosomes 10 and 11. A t(1;5) translocation was observed as well as several unbalanced rearrangements. CGH detected amplifications resulting from duplications of 2q, 6p and 13q. These changes could result in fusion gene products involved in carcinogenesis or the positions of putative oncogenes and tumour suppressor genes. A good correlation between conventional G-banding, CGH and M-FISH was observed. Copyright © 2001 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]

Published
2001
Full Text
View/download PDF

78. A high-temperature and high-pressure Raman spectroscopic study of CaGeO3 garnet.

Author

Chaplin, T. D., Ross, N. L., and Reynard, B.

Abstract

High-pressure and high-temperature Raman spectra of CaGeO3 tetragonal garnet have been collected to 11.5 GPa and 1225 K, respectively, in order to investigate possible intrinsic anharmonic behaviour in this phase. The Raman peak positions were observed to vary linearly with pressure and temperature within the ranges studied, with the higher-energy peaks showing larger P- and T-induced shifts than the low energy modes. The observed T-induced shifts are similar to those reported for grossular and andradite, while the observed P-induced shifts are generally larger than those of aluminosilicate and MgSiO3 majorite garnets (Gillet et al. 1992; Rauch et al. 1996) due to the larger bulk modulus of CaGeO3 garnet. The observed mode shifts of CaGeO3 garnet were used to determine the isothermal and isobaric mode Grüneisen parameters for this phase. These parameters are similar in value to those reported previously for grossular and andradite (Gillet et al. 1992). The calculated intrinsic anharmonic parameters, a i, for CaGeO3 garnet were determined to be nonzero, indicating significant anharmonic behaviour for this phase. These values, which range from −3.8 × 10−5 K−1 to −1.3 × 10−5 K−1, are also similar to those reported for andradite and grossular, but smaller than those determined for pyrope (Gillet et al. 1992). Hence, we expect MgSiO3 majorite to show greater anharmonicity than the germanate analogue studied by us. The anharmonic parameters determined for CaGeO3 tetragonal garnet may now be introduced into quasiharmonic vibrational heat capacity models to account for the observed anharmonic behaviour. [ABSTRACT FROM AUTHOR]

Published
2000
Full Text
View/download PDF

79. Dissimulation in phallometric testing of rapists' sexual preferences.

Author

Harris, Grant T., Rice, Marnie E., Harris, G T, Rice, M E, Chaplin, T C, and Quinsey, V L

Subjects
RAPISTS, SEXUAL orientation, PSYCHOLOGY, SEMANTICS, RESEARCH evaluation, RAPE, SEXUAL intercourse, VIOLENCE, PENILE erection, PSYCHOLOGICAL tests
Abstract

Sexual preferences of 38 rapists were assessed phallometrically with and without a semantic tracking task in a counterbalanced design. Four categories of audiotaped vignettes describing neutral interactions, consenting sex, rape, and nonsexual violence were employed as stimuli. In the semantic tracking task, participants were instructed to press one button when violent events were described in the vignette and another when sexual activities were described. Phallometric assessment with the semantic task better discriminated between rapists and non-sex-offender participants (from an earlier study) than the same assessment without the task. Among four rapists who had previous experience with phallometric testing, there was a very strong correlation between deviance scores and tracking accuracy. Results suggest that the semantic task may improve discriminant validity, particularly among sex offenders who have had previous experience with phallometric assessment. [ABSTRACT FROM AUTHOR]

Published
1999
Full Text
View/download PDF

85. DISCUSSION OF PAPERS 9620-9623. TSIM SHA TSUI CULTURAL CENTRE: INTRODUCTION; FOUNDATIONS AND SUPERSTRUCTURE; ROOF SYSTEM.

Author

GILL, C, PUGH, E, KELLY, P, HAPPOLD, E, GAMMON, J R A, CHAPLIN, T K, WAKEFIELD, D, COOK, M J, MORRISON, J, LATTO, W A, WESTWOOD, D D C, EALEY, T, and PRITCHARD, B P

Abstract

DISCUSSION Tsim Sha Tsui Cultural Centre: introduction; foundations and superstructure; roof system E. Happold, J. Morrison, T . Ealey,'E. Pugh, P. Kelly, D. Wakefield andM. J. Cook B. P. Pritchard, W. S. Atkins & Partners My first question concerns the shape the of building, which required ridges at the intermediate supportsof the cable roofs. However, the architect wanted a smooth unbroken profile. This meant variable depth claddingon top. Would it have been possible, if the ridges had been architecturally acceptable, to design the structure with justa very thin uniform depth cladding on top, or was it necessaryto have some sort of space above purely insulation for purposes,? 2...

Published
1992
Full Text
View/download PDF

88. The t(10;11) translocation in acute myeloid leukemia (M5) consistently fuses the leucine zipper motif of AF10 onto the HRX gene

Author

Chaplin, T., Bernard, O., Beverloo, H. B., Vaskar Saha, Hagemeijer, A., Berger, R., and Young, B. D.

Subjects
Adult, Oncogene Proteins, Fusion, RNA Splicing, Molecular Sequence Data, Polymerase Chain Reaction, Leukemia, Myelomonocytic, Acute, Translocation, Genetic, Proto-Oncogenes, Humans, Child, Leucine Zippers, Base Sequence, Chromosomes, Human, Pair 10, Chromosomes, Human, Pair 11, Infant, Exons, Histone-Lysine N-Methyltransferase, Middle Aged, Neoplasm Proteins, DNA-Binding Proteins, Genes, Child, Preschool, Leukemia, Monocytic, Acute, Myeloid-Lymphoid Leukemia Protein, Chromosomes, Human, Pair 17, Transcription Factors
Abstract

The gene on chromosome 10 at band p12 (AF10), involved in the t(10;11) translocation in acute myeloid leukemia, has been identified and shown to contain conserved zinc finger and leucine zipper domains. These regions are highly homologous to the equivalent regions on AF17, the gene involved in the t(11;17) translocations. A series of adult, childhood, and infant leukemias with either simple or complex versions of the t(10;11) has been examined by Southern analysis and shown to involve rearrangement to the HRX locus. Reverse transcriptase-polymerase chain reaction from either bone marrow or peripheral blood cells showed that HRX sequence was fused to AF10 sequence in all 8 cases and subsequent sequence analysis showed an in-frame fusion between the HRX and AF10 sequence. A consistent feature of these fusions was the juxtaposition of the leucine dimerization motif of AF10 onto the NH2-terminal region of HRX. The published data suggest that a similar conclusion can be drawn about the t(11;17) translocation, implying a critical role for this motif in the chimaeric HRX protein.

90. THE ISOLATION OF A YEAST ARTIFICIAL CHROMOSOME WHICH SPANS THE CHROMOSOME 11Q23 REGION INVOLVED IN A NUMBER OF TRANSLOCATIONS IN ACUTE LEUKEMIAS

Author

Mark Bower, Chaplin T, Das S, Kearney L, Gibbons B, Jh, Riley, Ta, Lister, and Bd, Young

Subjects
Adult, Male, Leukemia, Chromosomes, Human, Pair 11, Genes, Fungal, Chromosome Mapping, Infant, Polymerase Chain Reaction, Translocation, Genetic, Yeasts, Acute Disease, Humans, Female, Child, In Situ Hybridization, Aged, Gene Library, Teniposide

92. HEATHROW AIRPORT TERMINAL 4. 8966-8 DISCUSSION.

Author

BELL, G D, primary, WILLIAMS, G M J, additional, RUTTER, P A, additional, WILLIAMS, K, additional, CROOK, G M, additional, DOUGLAS, R H R, additional, SOWDEN, G H, additional, CHAPLIN, T K, additional, EMERY, J A, additional, ROYCE, N, additional, CALVERT, J T, additional, VEASEY, C M, additional, GOULD, H B, additional, CALVERLEY, M A A, additional, DAYA, R S, additional, and COMPTON, D H, additional

Published
1987
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results