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A high resolution dataset is one of the prerequisites for tea chrysanthemum detection with deep learning algorithms. This is crucial for further developing a selective chrysanthemum harvesting robot. However, generating high resolution datasets of the tea chrysanthemum with complex unstructured environments is a challenge. In this context, we propose a novel tea chrysanthemum – generative adversarial network (TC-GAN) that attempts to deal with this challenge. First, we designed a non-linear mapping network for untangling the features of the underlying code. Then, a customized regularization method was used to provide fine-grained control over the image details. Finally, a gradient diversion design with multi-scale feature extraction capability was adopted to optimize the training process. The proposed TC-GAN was compared with 12 state-of-the-art generative adversarial networks, showing that an optimal average precision (AP) of 90.09% was achieved with the generated images (512 × 512) on the developed TC-YOLO object detection model under the NVIDIA Tesla P100 GPU environment. Moreover, the detection model was deployed into the embedded NVIDIA Jetson TX2 platform with 0.1 s inference time, and this edge computing device could be further developed into a perception system for selective chrysanthemum picking robots in the future.

This paper aims to analyze the trading mechanism of China pilot carbon emission trading market and figure out whether the current market price is rational and valid. By applying directional distance function and stochastic frontier analysis method, we estimated the CO2 marginal abatement cost of 92 coal-fueled generators belonging to 27 power plants located in Shanghai which is one of the pilot carbon emission trading market cities in China. The result indicates that the market price will greatly affect the trading activities within carbon emission trading market. We conclude that the current market price is not rational for coal-fueled power industry which is high-carbon and high CO2 MAC industry. When the exchange of nationwide carbon trading market formally start, policymakers should impose carbon tax or carbon emission quota to adjust the market price and stimulate the CO2 emission reduction willingness of coal-fueled power plants.

Objective To analyze the clinical effects of single row anchor in repairing tears of the subscapularis muscle combined with the long head of the biceps tendon (LHBT) injury under arthroscopy. Methods From June 2016 to June 2017, the clinical data of 32 patients with subscapularis combined with biceps tendon injury were retrospectively analyzed. Preoperative MRI examination of the shoulder joint was performed to evaluate tendon injuries, and the subscapularis muscle was repaired with single row anchor under arthroscopy, and tenotomy or tenodesis was performed on the long head tendon of the biceps humerus in the intertubercular groove. The range of motion and the functional score of the shoulder joint before and after the operation were evaluated. All patients were followed up for at least 24 months. Results The mean follow‐up period was 28.8 months (range, 24–34 months). No infections occurred during the follow‐up period. The patients’ follow up exams showed significant improvement in postoperative shoulder joint flexion, external rotation, and internal rotation (P 0.05). Preoperative and postoperative scores were, respectively: visual analog scale score (1.7 ± 0.9 vs 1.8 ± 0.0.8) ASES score (81.3 ± 7.9 vs 80.1 ± 8.0) and constant score (80.9 ± 8.0 vs 80.1 ± 6.9). Conclusion Using single row anchor under arthroscopy to repair subscapularis combined with long head of biceps tendon injury yielded good results and high tendon healing rates were obtained.

ABSTRACT: An improved fast region-based convolutional neural network (RCNN) algorithm is proposed to improve the accuracy and efficiency of recognizing broilers in a stunned state. The algorithm recognizes 3 stunned state conditions: insufficiently stunned, moderately stunned, and excessively stunned. Image samples of stunned broilers were collected from a slaughter line using an image acquisition platform. According to the format of PASCAL VOC (pattern analysis, statistical modeling, and computational learning visual object classes) dataset, a dataset for each broiler stunned state condition was obtained using an annotation tool to mark the chicken head and wing area in the original image. A rotation and flip data augmentation method was used to enhance the effectiveness of the datasets. Based on the principle of a residual network, a multi-layer residual module (MRM) was constructed to facilitate more detailed feature extraction. A model was then developed (entitled here Faster-RCNN+MRMnet) and used to detect broiler stunned state conditions. When applied to a reinforcing dataset containing 27,828 images of chickens in a stunned state, the identification accuracy of the model was 98.06%. This was significantly higher than both the established back propagation neural network model (90.11%) and another Faster-RCNN model (96.86%). The proposed algorithm can complete the inspection of the stunned state of more than 40,000 broilers per hour. The approach can be used for online inspection applications to increase efficiency, reduce labor and cost, and yield significant benefits for poultry processing plants.

Focusing on the tendency of terrorist organizations to explosive attack, this article applied the institutional theory as the basis to explain the inherent logic of attack type similarity from the perspective of mimetic, coercive, and normative isomorphism. Subsequently, the study conducted an empirical analysis of the data onto 1825 terrorist organizations recorded in the Global Terrorism Database with the logistic regression method. The results show that: (1) Terrorist organizations will learn from pre-existing terrorist organizations' experiences, and mimetic isomorphism will promote explosive tendency; (2) Due to the normative isomorphism effect, terrorist groups' tendency to explosive attacks is weakened by their increased duration; (3) If terrorist organizations are hostile to a strong government, coercive isomorphism positively moderates the negative effects of increasing duration. The study suggests that counter-terrorism approaches such as destroying the learnable experience of attacks, addressing the root causes of terrorism, and maintaining a strong government may be helpful in stopping increasing terrorist activities, which is essential for reducing terrorist organizations' vivosphere, blocking the inter-flow and imitation between terrorist organizations, and ultimately interrupting the terrorist propagation chain.

Spinal cord injury (SCI) can result in sensorimotor impairments or disability. Studies of the cellular response to SCI have increased our understanding of nerve regenerative failure following spinal cord trauma. Biological, engineering and rehabilitation strategies for repairing the injured spinal cord have shown impressive results in SCI models of both rodents and non-human primates. Cell transplantation, in particular, is becoming a highly promising approach due to the cells’ capacity to provide multiple benefits at the molecular, cellular, and circuit levels. While various cell types have been investigated, we focus on the use of Schwann cells (SCs) to promote SCI repair in this review. Transplantation of SCs promotes functional recovery in animal models and is safe for use in humans with subacute SCI. The rationales for the therapeutic use of SCs for SCI include enhancement of axon regeneration, remyelination of newborn or sparing axons, regulation of the inflammatory response, and maintenance of the survival of damaged tissue. However, little is known about the molecular mechanisms by which transplanted SCs exert a reparative effect on SCI. Moreover, SC-based therapeutic strategies face considerable challenges in preclinical studies. These issues must be clarified to make SC transplantation a feasible clinical option. In this review, we summarize the recent advances in SC transplantation for SCI, and highlight proposed mechanisms and challenges of SC-mediated therapy. The sparse information available on SC clinical application in patients with SCI is also discussed.

PurposePrevious studies have shown that prophylactic cranial irradiation (PCI) can improve the survival of patients with limited-stage small cell lung cancer (LS-SCLC). PCI is recommended for patients who respond well to chemoradiotherapy. However, whether PCI could be extrapolated to the LS-SCLC patients in the modern era of MRI is unknown. This study aimed to explore the value of PCI in patients with LS-SCLC in the era of brain MRI.MethodsThis study included 306 patients with LS-SCLC at the Cancer Hospital of China Medical University. All patients received brain MRI at diagnosis and after radiochemotherapy to exclude brain metastases. A propensity score matching was performed to reduce the influence of potential confounders. Overall survival (OS), progression-free survival (PFS), and recurrence failure types were compared between PCI and non-PCI groups.ResultsAmong the 306 eligible patients, 81 underwent PCI, and 225 did not. After propensity score matching, there was no statistical difference in baseline data between the two groups, with 75 patients in each group. PCI did not achieve OS (median OS: 35 vs. 28 months, p = 0.128) or PFS (median PFS: 15 vs. 10 months, p = 0.186) benefits. During follow-up, 30 patients (20.0%) developed brain metastases, including 13 patients (17.3%) in the PCI group and 17 patients (22.7%) in the non-PCI group. Regarding death as a competitive risk, patients who received PCI had a lower cumulative incidence of brain metastasis than those who did not (3 years: 14.7% vs. 22.7%; Gray’s test, p = 0.007).ConclusionsWhen brain MRI was performed at diagnosis and pre-PCI, PCI could reduce the cumulative rate of brain metastases, but it did not achieve survival benefits for LS-SCLC patients.

Effects of total ionizing dose (TID) on the transient radiation upset sensitivity of commercial static random access memories (SRAMs) were investigated. Commercial SRAMs of three technology nodes were irradiated with Co-60 gamma rays for total dose effects and with pulsed x rays generated using a “Chen-Guang” accelerator for doserate effects. It is shown that TID irradiation and the memory pattern written to the memory array during TID irradiation can substantially affect the transient radiation upset sensitivity of all the SRAMs. First, the memory pattern written to the memory array during TID irradiation is more sensitive to pulsed x rays for all SRAMs. Second, the variation in transient radiation upset sensitivity with total dose has different tendencies for different SRAMs, but the static power current increased with the increase in total dose for all SRAMs. The increase in static power current with total dose and the increasing sensitivity of the memory pattern during TID irradiation are caused by the increasing TID induced leakage current, which results in an asymmetric noise margin of individual memory cells.

Background An ideal test for COVID-19 would combine the sensitivity of laboratory-based PCR with the speed and ease of use of point-of-care (POC) or home-based rapid antigen testing. We evaluated clinical performance of the Diagnostic Analyzer for Selective Hybridization (DASH) SARS-CoV-2 POC rapid PCR test. Methods We conducted a cross-sectional study of adults with and without symptoms of COVID-19 at four clinical sites where we collected two bilateral anterior nasal swabs and information on COVID-19 symptoms, vaccination, and exposure. One swab was tested with the DASH SARS-CoV-2 POC PCR and the second in a central laboratory using Cepheid Xpert Xpress SARS-CoV-2 PCR. We assessed test concordance and calculated sensitivity, specificity, negative and positive predictive values using Xpert as the “gold standard”. Results We enrolled 315 and analyzed 313 participants with median age 42 years; 65% were female, 62% symptomatic, 75% had received ≥2 doses of mRNA COVID-19 vaccine, and 16% currently SARS-CoV-2 positive. There were concordant results for 307 tests indicating an overall agreement for DASH of 0.98 [95% CI 0.96, 0.99] compared to Xpert. DASH performed at 0.96 [95% CI 0.86, 1.00] sensitivity and 0.98 [95% CI 0.96, 1.00] specificity, with a positive predictive value of 0.85 [95% CI 0.73, 0.96] and negative predictive value of 0.996 [95% CI 0.99, 1.00]. The six discordant tests between DASH and Xpert all had high Ct values (>30) on the respective positive assay. DASH and Xpert Ct values were highly correlated (R = 0.89 [95% CI 0.81, 0.94]). Conclusions DASH POC SARS-CoV-2 PCR was accurate, easy to use, and provided fast results (approximately 15 minutes) in real-life clinical settings with an overall performance similar to an EUA-approved laboratory-based PCR.

The nondestructive testing technique used to evaluate the quality of bolt support by detecting the axial force is suitable for the bolt with a short construction time. For the bolt in the support state for a long time period, it may lead to the detection of small axial force only and ignore the fact that the bolt has entered the necking fracture stage, and thus fail to detect safety hazards in time. Three sets of parallel tests were conducted to solve the misjudgment of detection results due to the reduction of axial force when the bolt was in the necking fracture stage. Bolt deformation characteristics and stress wave propagation characteristics in bolts under axial tensile stress were studied by using the bolt tensile and stress wave detection test system which was modified and built independently. The results showed that (1) The diameter of the necking position decreases continuously during the tensioning process of the bolt and the rate of decrease increases abruptly during the necking fracture stage. (2) Due to the bolt stretching, the stress wave propagates in the bolt with different degrees of reflection and transmission, resulting in the attenuation of the stress wave energy; the energy attenuation ratio of the stress wave signal in the necking fracture stage reaches 35%, and the energy attenuation ratio increases exponentially as the necking continues to occur. (3) The frequency distribution of the stress wave signal during the bolt stretching process is from scattered to concentrated, the dominant frequency is gradually prominent and changes from low frequency to high frequency, the high-frequency signal is more sensitive to the cracks and necking of the bolt, and the dominant frequency is between 9500 and 10,000 Hz. (4) The average error of the stress wave method is 1.945% and the maximum and minimum values are 4.12% and 0.51%, respectively. The method is promising and provides a reference for the study of nondestructive testing of bolt stress waves in field support.

Curcumin and its two derivatives of PB-3 and DY-1 were subjected to the forced degradation studies under the conditions of hydrolysis (acidic and alkaline), oxidation, photolysis, and thermal stress recommended by ICH Q1A (R2) by means of UPLC–DAD–MS/MS. Three analytes and their degradation products were separated on a column of ACQUITY UPLC® BEH C18 (100 mm × 2.1 mm, 1.7 μm) with an in-line filter prior to the column using acetonitrile and 10 mM ammonium acetate buffer (pH adjusted to 3.5) as a mobile phase. Both curcumin and DY-1 showed extensive degradation under alkaline condition and gave rise to two degradation products for curcumin and three for DY-1, respectively, while no degradation product was observed under other tested conditions. PB-3 was found to be unstable in acid and alkaline conditions, two degradation products in acidic hydrolytic condition and one in alkaline condition were obtained, while it was stable in photolytic, oxidative and thermal stress conditions. The degradation products of three analytes were characterized as follows by analyzing the mass fragmentation patterns of curcumin and mass analysis of the degradation products: ferulic acid and vanillin for curcumin, 3-methoxyl-4-[3-(1-tetrahydropyrrolyl)propoxyl] benzoic aldehyde, 2,2-di-(1-phenylmethyl)-3-oxo-5-[3-methoxyl-4-(3-tetrahydropyrolylpropoxyl)] pent-4-enoic acid, and tetrapyrolyl propoxyl methoxyl phenyl prop-2-enoic acid for PB-3, 3-oxo-5-[4-hydroxyl-5-methoxyl-3-(1-morpholinylmethyl)] pent-4-enoic aldehyde, 3-oxo-5-(4-hydroxyl-5-methoxyl-3-(1-morpholinylmethyl)) pent-4-enoic acid and 2-hydroxyl-3-methoxyl-5-(7-(3-methoxyl-4-hydroxylphenyl)-3,5-dioxo-4,4-dimethyl hept-1,6-dienyl) benzoic acid for DY-1, respectively. The degradation pathways of curcumin and its derivatives were presented in addition. Keywords: Curcumin, PB-3, DY-1, UPLC–DAD–MS/MS, Degradation products

For the purpose of studying the influence of fiber on the negative Poisson’s ratio effect of foam concrete, a concave hexagonal unit cell structure of polypropylene fiber foam concrete was proposed. The effects of different fiber volume contents on the structural mechanical parameters, Poisson’s ratio, and energy absorption capacity of the unit cells were studied by static compression of concave hexagonal unit cells and cube specimens. The results show that the compressive strength of foam concrete is reduced by adding polypropylene fiber, and the peak stress of concave hexagonal unit cells decreases less rapidly than that of cube specimens. The proper amount of polypropylene fiber can enhance the deformation ability of the unit cells in foam concrete, and the Poisson’s ratio of the unit cells in foam concrete with 1.5% fiber content is the lowest. In the process of failure of concave hexagonal unit cells, the failure phenomenon is mainly concentrated on the concave surfaces on both sides, and the cracks are distributed in the form of “upper left and lower right” or “lower left and upper right”. When the content of polypropylene fiber is 0.5%, the total energy absorbed by the concave hexagonal cells in the compression deformation process increases by 12.98%.

ABSTRACT Carbapenem-resistant Klebsiella pneumoniae strains cause severe infections that are difficult to treat. The production of carbapenemases such as the K. pneumoniae carbapenemase (KPC) is a common mechanism by which these strains resist killing by the carbapenems. However, the degree of phenotypic carbapenem resistance (MIC) may differ markedly between isolates with similar carbapenemase genes, suggesting that our understanding of the underlying mechanisms of carbapenem resistance remains incomplete. To address this problem, we determined the whole-genome sequences of 166 K. pneumoniae clinical isolates resistant to meropenem, imipenem, or ertapenem. Multiple linear regression analysis of this collection of largely blaKPC-3-containing sequence type 258 (ST258) isolates indicated that blaKPC copy number and some outer membrane porin gene mutations were associated with higher MICs to carbapenems. A trend toward higher MICs was also observed with those blaKPC genes carried by the d isoform of Tn4401. In contrast, ompK37 mutations were associated with lower carbapenem MICs, and extended spectrum β-lactamase genes were not associated with higher or lower MICs in carbapenem-resistant K. pneumoniae. A machine learning approach based on the whole-genome sequences of these isolates did not result in a substantial improvement in prediction of isolates with high or low MICs. These results build upon previous findings suggesting that multiple factors influence the overall carbapenem resistance levels in carbapenem-resistant K. pneumoniae isolates. IMPORTANCE Klebsiella pneumoniae can cause severe infections in the blood, urinary tract, and lungs. Resistance to carbapenems in K. pneumoniae is an urgent public health threat, since it can make these isolates difficult to treat. While individual contributors to carbapenem resistance in K. pneumoniae have been studied, few reports explore their combined effects in clinical isolates. We sequenced 166 clinical carbapenem-resistant K. pneumoniae isolates to evaluate the contribution of known genes to carbapenem MICs and to try to identify novel genes associated with higher carbapenem MICs. The blaKPC copy number and some outer membrane porin gene mutations were associated with higher carbapenem MICs. In contrast, mutations in one specific porin, ompK37, were associated with lower carbapenem MICs. Machine learning did not result in a substantial improvement in the prediction of carbapenem resistance nor did it identify novel genes associated with carbapenem resistance. These findings enhance our understanding of the many contributors to carbapenem resistance in K. pneumoniae.

PurposeThis study aimed to evaluate the survival outcomes of whole brain radiotherapy (WBRT) compared to whole brain radiotherapy plus local radiation boost (WBRT + boost), and further identify whether higher biologically effective dose (BED) of WBRT + boost translates into a survival benefit in small cell lung cancer (SCLC) patients with brain metastasis (BM).MethodsSCLC patients with BM from January 1, 2012, to December 31, 2019, were retrospectively analyzed. Overall survival (OS) and intracranial progression-free survival (iPFS) were evaluated by the Kaplan–Meier method and compared by the log-rank test. Univariate and multivariate regression analyses of prognostic factors for OS were performed using Cox proportional hazards regression models. The cutoff value of BED was determined by the receiver operating characteristic (ROC) curve analysis.ResultsAmong the 180 eligible patients, 82 received WBRT + boost and 98 received WBRT. Both OS and iPFS in the WBRT + boost group were significantly superior to those in the WBRT group (median OS: 20 vs. 14 months, p = 0.011; median iPFS: 16 vs. 10 months, p = 0.003). At a cutoff value of 58.35 Gy in the WBRT + boost group, 52 for the high-BED (>58.35 Gy) group, 30 for the low-BED (≤58.35 Gy) group. High BED was significantly associated with improved OS and iPFS compared with low BED in the WBRT + boost group (median OS: 23 vs. 17 months, p = 0.002; median iPFS: 17 vs. 10 months, p = 0.002).ConclusionsCompared with WBRT alone, WBRT + boost improved OS and iPFS in SCLC patients with BM. High BED (>58.35 Gy) for WBRT + boost may be a reasonable consideration for SCLC patients with BM.

Cardiovascular diseases (CVDs) are the leading cause of deaths worldwide with regulated cell death playing an important role in cardiac pathophysiology. However, the classical mode of cell death cannot fully explain the occurrence and development of heart disease. In recent years, much research has been performed on ferroptosis, a new type of cell death that causes cell damage and contributes to the development of atherosclerosis, myocardial infarction, heart failure, and other diseases. In this review, we discuss the role of different organelles in ferroptosis and also focus on the relationship between autophagy and ferroptosis. Additionally, we describe the specific mechanism by which ferroptosis contributes to the development of CVD. Finally, we summarize the current research on ferroptosis-related pathway inhibitors and the applications of clinically beneficial cardiovascular drugs.

Electromagnetic bi-directional micropumps (EMBM) are indispensable for the development of portable devices which enable fluid transportation in forward and reverse directions. However, the high disposal cost of rare-earth magnets attached to the fluidic part and the low pump density due to multiple motors limit their practical application in disposable multi-channel microfluidic applications such as droplet-based oscillatory-flow (DBOF) rapid PCR amplification. Therefore, this paper presented a low-cost, disposable, high-pump-density EMBM. To reduce the disposal cost, we separated the magnets from the disposable fluidic part and used cylindrical holes to store and guide the magnet, which resulted in the ability to reuse all the magnets. To increase the pump density, we used the combination of one motor and one multi-pole ring magnet to drive several channels simultaneously. A proof-of-concept prototype with a pump density of 0.28 cm−2 was fabricated and experimentally evaluated. The fabricated micropump exhibited a maximum flow rate of 0.86 mL/min and a maximum backpressure of 0.5 kPa at a resonant frequency around 50 Hz. The developed multi-channel micropump with reusable magnets is highly beneficial to the development of low-cost and high-throughput rapid PCR amplification microchips and therefore can have a significant impact on timely infectious disease recognition and intervention.

Abstract Background Initiating early effective antimicrobial therapy is the most important intervention demonstrated to decrease mortality in patients with gram-negative bacteremia with sepsis. Rapid MIC-based susceptibility results make it possible to optimize antimicrobial use through both escalation and de-escalation. Method We prospectively evaluated the performance of the Accelerate Pheno™ system (AXDX) for identification and susceptibility testing of gram-negative species and compared the time to result between AXDX and routine standard of care (SOC) using 82 patient samples and 18 challenge organisms with various confirmed resistance mechanisms. The potential impact of AXDX on time to antimicrobial optimization was investigated with various simulated antimicrobial stewardship (ASTEW) intervention models. Results The overall positive and negative percent agreement of AXDX for identification were 100 and 99.9%, respectively. Compared to VITEK® 2, the overall essential agreement was 96.1% and categorical agreement was 95.4%. No very major or major errors were detected. AXDX reduced the time to identification by an average of 11.8 h and time to susceptibility by an average of 36.7 h. In 27 patients evaluated for potential clinical impact of AXDX on antimicrobial optimization, 18 (67%) patients could potentially have had therapy optimized sooner with an average of 18.1 h reduction in time to optimal therapy. Conclusion Utilization of AXDX coupled with simulated ASTEW intervention notification substantially shortened the time to potential antimicrobial optimization in this cohort of patients with gram-negative bacteremia. This improvement in time occurred when ASTEW support was limited to an 8-h coverage model.

In RV transmission system (RVTS), friction is closely related to rotational speed and angular position. However, classical friction models do not consider the influence of angular position on friction, resulting in limited accuracy in describing the RVTS frictional behavior. For this reason, this paper proposes an angular position-based two-stage friction model for RVTS, and achieves a more accurate representation of friction of RVTS. The proposed model consists of two parts, namely pre-sliding model and sliding model, which are divided by the maximum elastic deformation recovery angle of RVTS obtained from loading-unloading tests. The pre-sliding friction behavior is regarded as a spring model, whose stiffness is determined by the angular position and the acceleration when the velocity crosses zero, while the sliding friction model is established by the angular-segmented Stribeck function, and the friction parameters of the adjacent segment are linearly smoothed. A feedforward compensation based on the proposed model was performed on the RVTS, and its control performance was compared with that using the classical Stribeck model. The comparison results show that when using the proposed friction model, the low-speed-motion smoothness of the RVTS can be improved by 14.2%, and the maximum zero-crossing speed error can be reduced by 37.5%, which verifies the validity of the proposed friction model, as well as the compensation method.

Introduction Acute myocardial infarction (AMI) has become one of the major causes of mortality and morbidity in China. However, little is known about the characteristics, medical care and outcomes of patients with AMI in Northeastern China. The Acute Myocardial Infarction Study in Northeastern China (AMINoC) is aimed at obtaining timely real-world knowledge in terms of characteristics, clinical care and outcomes of patients with AMI and at providing care-quality improvement efforts in Northeastern China.Methods and analysis The AMINoC is a real-world, prospective, multicentre cohort study. The study selected 20 hospitals using stratified cluster sampling from different levels of hospitals among nine districts throughout Jilin Province. Hospitalised patients with a primary diagnosis of AMI in each site are consecutively enrolled for 1 year. Demographic characteristics, clinical data, treatments, outcomes and cost are collected by local investigators. Patient follow-up after discharge is planned for up to 2 years.Ethics and dissemination The protocol has been approved by the ethics committee at the Second Hospital of Jilin University. The findings of this study will be published in peer-reviewed journals and medical conferences.Trial registration NCT04451967.

In order to make certain of the blocking impact of the ultra-wideband electromagnetic pulse (UWB EMP) on a radio communication station, an EMP injection test was conducted to study the blocking interference of a digital radio station under a step pulse train and double exponential pulse train. Combined with waveform parameters such as the pulse repetition rate (PRR), the concept of influence time when the UWB EMP was irradiated on the equipment under test (EUT) was introduced, and the model was established. According to the experimental results, the blocking impact of the EUT under the UWB EMP depended on factors such as waveform, PRR, and amplitude. The relationship between the PRR and the sensitive bit error rate could be determined by the effect test. In the case of a low PRR, the sensitive voltage threshold of the EUT was irrelevant to the PRR, and the effect was the monopulse effect. When the PRR reached above the reciprocal of the influence time, the sensitive voltage threshold of the EUT decreased with the increase in the PRR, which conformed to the cumulative multiple pulse effects.

Objectives:. Determine the variation in outcomes and respiratory mechanics between the subjects who are intubated earlier versus later in their coronavirus disease 2019 course. Design:. Retrospective cohort study. Setting:. Northwestern Memorial Hospital ICUs. Patients:. All patients intubated for coronavirus disease 2019 between March 2020 and June 2020. Interventions:. Patients were stratified by time to intubation: 30 subjects were intubated 4–24 hours after presentation and 24 subjects were intubated 5–10 days after presentation. Baseline characteristics, hospitalization, ventilator mechanics, and outcomes were extracted and analyzed. Ten clinically available CT scans were manually reviewed to identify evidence of pulmonary vascular thrombosis and intussusceptive angiogenesis. Measurements and Main Results:. Median time from symptom onset to intubation was significantly different between the early and late intubation cohorts, with the latter being intubated later in the course of their illness (7.9 vs 11.8 d; p = 0.04). The early intubation cohort had a lower mortality rate than the late intubation cohort (6% vs 30%, p < 0.001) without significantly different respiratory mechanics at the time of intubation. The late intubation cohort was noted to have higher dead space ratio (0.40 vs 0.52; p = 0.03). On review of CT scans, the late intubation cohort also had more dilated peripheral segments on imaging (two segments vs five segments). Conclusions:. The question as to whether delaying intubation is beneficial or harmful for patients with coronavirus disease 2019-induced hypoxemic respiratory failure has yet to be answered. As our approaches to coronavirus disease 2019 continue to evolve, the decision of timing of intubation remains paramount. Although noninvasive ventilation may allow for delaying intubation, it is possible that there are downstream effects of delayed intubation that should be considered, including the potential for pulmonary vascular thrombosis and intussusceptive angiogenesis with delayed intubation.

The harmful effects (water hammer wave, flying stone, and broken quality of concrete) produced in the process of underwater drilling and blasting are the key factors affecting the safety of underwater drilling and blasting. In fact, the harm caused by the water hammer wave is the most significant. As a consequence, it is of great significance to study the propagation law of water hammer waves. Based on the background of the cofferdam demolition project at the inlet section of Shibishan Central Canal in Ningguo City, China, a three-dimensional numerical model was established based on Coupled Eulerian–Lagrangian (CEL) method. Besides, the propagation law of water hammer waves at different water depths with different millisecond times was studied. Meanwhile, the reduction effect of the water hammer wave at different positions of the bubble curtain was analyzed. The results showed that, in the direction of the minimum resistance line of the charge, the attenuation law of the water hammer wave is accorded with the Cole formula and attenuated exponentially. The attenuation speed of the water hammer wave increased at first and then decreased with the increase of the millisecond time. In addition, the attenuation rate of the peak pressure was the fastest when the millisecond interval 30 ms was used. The attenuation of the water hammer wave at different water depths decreased at first and then decreased with the increase of water depth. The attenuation law of the water hammer wave decreased linearly with the increase of the distance between the bubble curtain and the charge. The research results can provide particular guiding significance for similar on-site construction.

To study the impact of mining of the lower protective layer on the deformation and failure characteristics of the upper roadway, these characteristics of an 879 gas drainage roadway were studied and analyzed during the mining of the II 1051 working face of the Zhuxianzhuang coal mine using similar simulation experiments and numerical simulation methods. The results indicate that with the continuous excavation of the working face, the range of impact of the mining stress gradually spreads and exceeds the level of the roadway. At the present time, the roadway is in a mining stress-rising area. The two sides of the roadway are sheared, and the roof and floor are under tension–shear composite failure. The floor is the most gravely damaged—the depth of its damage is 2.5 m, and the depths of damage on either side and of the roof are approximately 1–2 m. During the advancing process of the working face, the deformation of the roadway increases slowly at first, then increases sharply, and tends to be stable thereafter. The deformation of the floor is the largest, followed by those of the two sides and the roof; the values are 800, 400, and 300 mm, respectively.

Background: The rapid spread of SARS-CoV-2, the causative agent of Coronavirus disease 2019 (COVID-19), has been accompanied by the emergence of distinct viral clades, though their clinical significance remains unclear. Here, we aimed to investigate the phylogenetic characteristics of SARS-CoV-2 infections in Chicago, Illinois, and assess their relationship to clinical parameters. Methods: We performed whole-genome sequencing of SARS-CoV-2 isolates collected from COVID-19 patients in Chicago in mid-March, 2020. Using these and other publicly available sequences, we performed phylogenetic, phylogeographic, and phylodynamic analyses. Patient data was assessed for correlations between demographic or clinical characteristics and virologic features. Findings: The 88 SARS-CoV-2 genome sequences in our study separated into three distinct phylogenetic clades. Clades 1 and 3 were most closely related to viral sequences from New York and Washington state, respectively, with relatively broad distributions across the US. Clade 2 was primarily found in the Chicago area with limited distribution elsewhere. At the time of diagnosis, patients infected with Clade 1 viruses had significantly higher average viral loads in their upper airways relative to patients infected with Clade 2 viruses, independent of disease severity. Interpretation: These results show that multiple variants of SARS-CoV-2 were circulating in the Chicago area in mid-March 2020 that differed in their relative viral loads in patient upper airways. These data suggest that differences in virus genotype can impact viral load and may influence viral spread. Funding: Dixon Family Translational Research Award, Northwestern University Clinical and Translational Sciences Institute (NUCATS), National Institute of Allergy and Infectious Diseases (NIAID), Lurie Comprehensive Cancer Center, Northwestern University Emerging and Re-emerging Pathogens Program.

Medicinal chrysanthemum detection is one of the desirable tasks of selective chrysanthemum harvesting robots. However, it is challenging to achieve accurate detection in real time under complex unstructured field environments. In this context, we propose a novel lightweight convolutional neural network for medicinal chrysanthemum detection (MC-LCNN). First, in the backbone and neck components, we employed the proposed residual structures MC-ResNetv1 and MC-ResNetv2 as the main network and embedded the custom feature extraction module and feature fusion module to guide the gradient flow. Moreover, across the network, we used a custom loss function to improve the precision of the proposed model. The results showed that under the NVIDIA Tesla V100 GPU environment, the inference speed could reach 109.28 FPS per image (416 × 416), and the detection precision (AP50) could reach 93.06%. Not only that, we embedded the MC-LCNN model into the edge computing device NVIDIA Jetson TX2 for real-time object detection, adopting a CPU–GPU multithreaded pipeline design to improve the inference speed by 2FPS. This model could be further developed into a perception system for selective harvesting chrysanthemum robots in the future.

Methicillin-resistant Staphylococcus aureus (SA) bacteremia is responsible for over 10,000 deaths in the hospital setting each year. Both conventional CD4+ T cells and γδ T cells play protective roles in SA infection through secretion of IFN-γ and IL-17. However, the role of other unconventional T cells in SA infection is largely unknown. Natural killer T (NKT) cells, a subset of innate-like T cells, are activated rapidly in response to a wide range of self and microbial lipid antigens presented by MHC I-like molecule CD1d. NKT cells are divided into two groups, invariant NKT (iNKT) and type II NKT cells, based on TCR usage. Using mice lacking either iNKT cells or both types of NKT cells, we show that both NKT cell subsets are activated after systemic SA infection and produce IFN-γ in response to SA antigen, however type II NKT cells are sufficient to control bacterial burden and inflammatory infiltrate in infected organs. This protective capacity was specific for NKT cells, as mice lacking mucosal associated invariant T (MAIT) cells, another innate-like T cell subset, had no increased susceptibility to SA systemic infection. We identify polar lipid species from SA that induce IFN-γ production from type II NKT cells, which requires both CD1d-TCR engagement and IL-12 production by antigen presenting cells. We also demonstrate that a population of T cells enriched for type II NKT cells are increased in PBMC of SA bacteremic patients compared to healthy controls. Therefore, type II NKT cells perform effector functions that enhance control of SA infection prior to conventional T cell activation and recognize SA-derived lipid antigens. As CD1d is highly conserved in humans, these CD1d-restricted SA lipid antigens could be used in the design of next generation SA vaccines targeting cell-mediated immunity.

Background: The outbreak of coronavirus disease 2019 (COVID-19) in December 2019 overlaps with the flu season. Methods: We compared clinical and laboratory results from 719 influenza and 973 COVID-19 patients from January to April 2020. We compiled laboratory results from the first 14 days of the hospitalized patients using parameters that are most significantly different between COVID-19 and influenza and hierarchically clustered COVID-19 patients. Findings: Compared to influenza, patients with COVID-19 exhibited a continued increase in white blood cell count, rapid decline of hemoglobin, more rapid increase in blood urea nitrogen (BUN) and D-dimer, and higher level of alanine transaminase, C-reactive protein, ferritin, and fibrinogen. COVID-19 patients were sub-classified into 5 clusters through a hierarchical clustering analysis. Medical records were reviewed and patients were risk stratified based on the clinical outcomes. The cluster with the highest risk showed 27·8% fatality, 94% ICU admission, 94% intubation, and 28% discharge rates compared to 0%, 38%, 22%, and 88% in the lowest risk cluster, respectively. Patients in the highest risk cluster had leukocytosis including neutrophilia and monocytosis, severe anemia, increased red blood cell distribution width, higher BUN, creatinine, D-dimer, alkaline phosphatase, bilirubin, and troponin. Interpretation: There are significant differences in the clinical and laboratory courses between COVID-19 and influenza. Risk stratification in hospitalized COVID-19 patients using laboratory data could be useful to predict clinical outcomes and pathophysiology of these patients. Funding: National Institute of Diabetes and Digestive and Kidney Disease, Department of Defense, and National Heart, Lung, and Blood Institute.

Although tungsten is considered as one of the primary candidates for plasma-facing material (PFM) in future fusion reactors, blistering induced by plasma irradiation adversely affects fusion reactors. To overcome blistering, in this study, we proposed an approach to reduce the blistering by releasing hydrogen or helium (H/He) through the grooved surfaces of PFM. In order to verify the feasibility of this approach and the effectiveness of the transverse release of H/He, the deep grooves as the channel simulants on the tungsten surface were fabricated with different spacing using focused ion beam (FIB). The result of helium irradiation shows that the presence of grooved surfaces can reduce blisters and inhibit the rapid growth of He blisters. In addition, the blistering reduced significantly with decreasing groove spacing, especially when the groove spacing is less than 2 µm. In addition, He-induced blistering shows obvious crystal orientation dependence, and the (001) surface shows the most serious radiation damage.

Fractures are crucial for unconventional hydrocarbon exploitation, but it is difficult to accurately observe the 3D spatial distribution characteristics of fractures. Microtomography (micro-CT) technology makes it possible to observe the 3D structures of fractures at micro-scale. In this study, micron-CT scanning is conducted on multiple mud-shale samples of source rocks in the Permian Lucaogou Formation, Junggar Basin. The Avizo® software is applied to process and segment the micron-CT images, so as to obtain the 3D fracture structure model inside rock core. Therefore, the independently-developed CTSTA program is adopted to quantitatively describe the micro-fractures inside rock core, including fracture dimension, extension direction and extension scale. Meanwhile, this study summarizes the classification characteristics of fractures and their anisotropy. On this basis, the fractal dimensions of fractures can also be extracted. Previous studies show that the geometric features of fractures have self-similarity at large and small scales, which can be described by exponential laws; and the fractal dimension is a typical exponent. Through the quantitative description or characterization of 3D fractures at micro-scale, the distribution characteristics of fractures in large scales could be known. Keywords: Tight reservoir, Microtomography, Fracture, Characterization, Fractal dimension

Abstract Background Inflammation and biofilm formation by Staphylococcus aureus (S. aureus) are common causes of periprosthetic infection and loosening. Recently, we identified that forsythiaside is bacteriostatic for S. aureus and methicillin-resistant S. aureus (MRSA). The purpose of the present study was to examine the effect of forsythiaside on S. aureus and MRSA adhesion and biofilm formation on the surface of titanium alloy, which is a popular material for orthopedic joint prostheses. Methods Two strains of S. aureus and MRSA were used for in vitro experiments. The spread plate method, confocal laser scanning microscopy (CLSM), and scanning electron microscopy (SEM) were used to characterize antimicrobial activity of forsythiaside. Real-time polymerase chain reaction (RT-PCR) and western blotting were used to investigate the inhibitory level of forsythiaside required for titanium-associated inflammation. Results Direct colony counting showed that 16 μg/mL forsythiaside significantly inhibited S. aureus and MRSA adhesion on titanium alloy discs in 2 h. CLSM and SEM showed that higher concentrations (> 30 mg/mL) of forsythiaside effectively inhibited the adhesion of S. aureus and MRSA on the surface of the titanium disc in 24 h. Forsythiaside was capable of attenuating Ti-induced activation of nuclear factor-κB signaling, targeting IκB kinase-α (IKKα) kinases of macrophages, and influencing the expression of NF-κB downstream cytokines. Conclusions These observations suggest that forsythiaside is a potential agent for the treatment of Ti implant-associated infection and inflammation.

Network security assessment helps to grasp the current and future risks of the network information system clearly,and gives corresponding security suggestions and countermeasures.Model of network security assessment and analysis is an important research direction and content,for which there is no review to give a comprehensive summary or analysis.A review of common network security evaluation and analysis model was given.The model that focus on attack was introduced,such as the attack tree,attack graph and kill chain,then the model that focus on network defense or offensive and defensive interaction was explained,such as attack surface,cyber epidemic model,Petri nets and automation machine,giving introduction to the basic concept,application field,modeling analysis process,advantages and disadvantages of the models.Then,typical examples were given to illustrate the application of the models in network defense technology evaluation and analysis.

Abstract Background Drug-eluting balloons (DEB) have significant value for treating coronary artery disease (CAD). However, the merits of using DEB versus drug-eluting stents (DES) to treat CAD remain controversial. Herein, we conducted a meta-analysis to compare the safety and efficacy of DEB and DES for treatment of CAD. Methods We searched MEDLINE, EMBASE, and CENTRAL databases for eligible trials comparing DEB with DES for treatment of CAD. The primary endpoint was major adverse cardiac events (MACE); the secondary endpoints included in-lesion late lumen loss (LLL), binary restenosis (BR), myocardial infarction (MI), target lesion revascularization (TLR) and mortality. Results Twenty-three trials with a total of 2712 patients were included. There were no significant differences in the primary endpoint of MACE between the DEB and DES groups (Risk Ratio (RR) 1.19; 95% confidence interval (CI) (0.87, 1.63); P = 0.27), or in the clinical outcomes of each of MACE’s components, including TLR, MI and mortality. However, efficacy was significantly different between the DEB and DES groups, especially when we compared DEB to second-generation DES: in-lesion LLL (Mean Difference (MD) 0.11; (0.01, 0.22); P = 0.03); binary restenosis (RR 1.46; (1.00, 2.13); P = 0.05). Conclusions DEB is equivalent to DES in terms of safety for managing CAD, and DEB may be considered as an alternative choice for treatment of CAD.

Abstract Background Recently, increased tumor incidence and cancer-related mortality have been reported among patients with obstructive sleep apnea (OSA). Intermittent hypoxia (IH), the hallmark feature of OSA, contributes to the metastasis of tumors. However, the molecular mechanisms by which tumor metastasis is accelerated by OSA-like IH remain to be elucidated. Methods C57BL/6 J male mice were subjected to intravenous injection of B16F10 melanoma cells before receiving IH treatment. Then, the animals were randomly distributed into three groups (n = 8 each): normoxia (N) group, IH group, and antioxidant tempol group (IHT, exposed to IH after treatment with tempol). After the mice were sacrificed, the number and weight of lung metastatic colonies were assessed. The lung tissues with tumor metastasis were analyzed for markers of oxidative stress and inflammation and for HIF-1α using western blotting and real-time PCR (qRT-PCR). The level of reactive oxygen species (ROS) in B16F10 cell was also assessed after N, IH and IH with tempol treatments. Results Compared with normoxia, IH significantly increased the number and weight of mouse lung metastatic colonies. Treatment of B16F10 cells with IH significantly enhanced ROS generation. Lung tissues with tumor metastasis provided evidence of increased oxidative stress, as assessed by p22phox and SOD mRNA levels and the NRF2 protein level, as well as increased inflammation, as assessed by TNF-α and IL-6 mRNA levels and the NF-κB P65 protein level. HIF-1α protein levels were increased in response to IH treatment. Tempol, an important antioxidant, ameliorated IH-induced melanoma lung metastasis in mice and reduced oxidative stress and inflammation responses. Conclusions These results support the hypothesis that oxidative stress and inflammation responses play an important role in the pathogenesis of OSA-like IH-induced melanoma lung metastasis in mice. Antioxidant intervention provides a novel strategy for the prevention and treatment of cancer in OSA populations.

In this paper, a new expression recognition approach is presented based on cognition and mapped binary patterns. At first, the approach is based on the LBP operator to extract the facial contours. Secondly, the establishment of pseudo-3-D model is used to segment the face area into six facial expression sub-regions. In this context, the sub-regions and the global facial expression images use the mapped LBP method for feature extraction, and then use two classifications which are the support vector machine and softmax with two kinds of emotion classification models the basic emotion model and the circumplex emotion model. At last, we perform a comparative experiment on the expansion of the Cohn-Kanade (CK +) facial expression data set and the test data sets collected from ten volunteers. The experimental results show that the method can effectively remove the confounding factors in the image. And the result of using the circumplex emotion model is obviously better than the traditional emotional model. By referring to relevant studies of human cognition, we verified that eyes and mouth express more emotion.

As critical components in SDN,controllers are prone to suffer from a series of potential attacks which result in system crashes.To prevent the compromise caused by single failure of controller or flow-tampering attacks,Mcad-SA,an aware decision-making security architecture with multiple controllers was proposed,which coordinates heterogeneous controllers internally as an“associated”controller.This architecture extends existing control plane and takes advantage of various controllers’merits to improve the difficulty and cost of probes and attacks from attackers.In this framework,flow rules distributed to switches are no longer relying on a single controller but according to the vote results from the majority of controllers,which significantly enhances the reliability of flow rules.As to the vote process of flow rules,segmentation and grading is adopted to pick up the most trustful one from multiple flow rules and implement flow consistency.This mechanism avoids comparison between rules via bit by bit which is impractical among several controllers.Theory analysis and simulation results demonstrates the effectiveness,availability and resilience of the proposed methods and their better security gain over general SDN architectures.

Abstract Background We evaluated the effectiveness of targeted antimicrobial prophylaxis in transrectal ultrasound guided prostate biopsy (TRUSP). Methods A prospective, non-randomized cohort study was conducted. Rectal swab cultures plated on non-selective blood agar and on selective MacConkey agar supplemented with ciprofloxacin identified ciprofloxacin-susceptible and –resistant gram-negative bacteria (CS-GNB and CR-GNB). Patients with CS-GNB received ciprofloxacin while those with CR-GNB received directed prophylaxis. Infectious complications were defined clinically and microbiologically within 30 days after TRUSP. Data were derived at 7 and 30 days post procedure by questionnaires and electronic medical records. We hypothesized that there would be no difference in the infectious outcomes among the CS and CR groups. Results From November 1, 2012 to March 31, 2015, 510 men completed the study; 430 (84.3%) had CS-GNB and 80 (15.7%) had CR-GNB. 484 (94.9%) completed the study per protocol, while 26 (5.1%) had an intention-to-treat (ITT) analysis. Of the 484, 475 (98.1%) had no infections, nine (1.9%) had infections, six of which (1.2%) were culture-proven (CP). The nine infections were as follows: five (1.0%) uncomplicated UTIs, one (0.2%) complicated UTI, and three (0.6%) urosepsis. One case of uncomplicated UTI and two cases of urosepsis were not CP, but were diagnosed clinically. ITT outcomes were similar. The infection rates were not statistically different between the CS-and CR-GNB patients (p-value = 0.314; 95% CI 0.8–3.3). The four patients with complicated UTIs or sepsis were hospitalized for a mean of 2.6 days and discharged without sequelae. Of the nine infections, three were antimicrobial prophylaxis failures (two ciprofloxacin and one amikacin); three were likely due to failure of the collection or processing of the rectal swab or increasing bacterial resistance between the time of swab collection and biopsy, and three developed clinical infections with no isolate recovered. Conclusions Targeted antimicrobial prophylaxis follows the principles of antimicrobial stewardship and achieved a low rate of infectious complications with limited morbidity and no sequelae. This individualized method of prophylaxis may be widely applied. Further studies are needed to explore reasons for targeted prophylaxis failure and to determine comparative efficacy of non-ciprofloxacin-containing targeted prophylaxis regimens. Trial registration ClinicalTrials.gov. NCT01659866 . Registered 9 July 2012. First patient enrolled 1 November 2012.

The physical reason behind the much more rapid short-term annealing progress in the presence of electrons in P-type silicon irradiated by pulse neutrons is investigated. Continuum equations coupled with defect reactions are used for the numerical simulation on the temporary evolutions of defects at different electron injection ratios. It is indicated that electron injections can significantly accelerate the creations of boron and carbon interstitials, but may have little influence on the creation of the vacancy-oxygen complex. The calculations on the relative concentrations of interstitials and vacancies in different charge states via the electron injection ratio show that more and more interstitials in the doubly positive charge state change into neutral ones, and neutral vacancies are always dominant with an increase in the electron concentration. The thermal diffusion coefficient of neutral interstitials is much larger than the ones of interstitials in other charge states. As a consequence, normal ionization enhanced diffusion for interstitials in the presence of electrons can significantly accelerate the short-term annealing progress in P-type silicon. Ionization enhanced diffusion coefficients for interstitials due to athermal diffusion are further estimated using the Bourgoin mechanism, and results indicate that the diffusion coefficients of positively charged interstitials are improved by several orders of magnitude when compared to their corresponding thermal diffusion coefficients. However, the production of boron and carbon interstitials is only accelerated by about one order of magnitude, which would be ascribed into such large thermal diffusion coefficients of neutral interstitials.

Due to transmission backlash of precise cable drive in electro-optical targeting system, it is difficult to predict force and pointing precisely during target detection. This article aims to develop a rigorous model for predicting the cable tension and transmission backlash of precise cable drive in electro-optical targeting system to better evaluate the performance in the preliminary design stages. The model of transmission backlash in different phase is established by satisfying the system’s geometric and equilibrium constraints, which is applicable to cable drive systems with any external load, pretension, and motion trajectories. Simulations are conducted to reveal the changes of cable deformation, cable tension, and deflection angles. The model is validated by experiments using a suitable experimental setup, predicting transmission backlash with the error level of 8.27%. The sensitivities of the preload force and external load are tested, and corresponding results are in good agreement with the theoretical ones. The backlash plunges dramatically when the preload force is small and flattens out when the preload force is larger than the threshold value. A proper preload force should be applied to keep the backlash in stable state. The threshold value could be predicted by the proposed model. The method presented in this article could provide a theoretic guide for system design and nonlinearity compensation.

Shear deformation of a solid-fluid, two-phase material induces a fluid segregation process that produces fluid-enriched bands and fluid-depleted regions, and a crystallographic preferred orientation (CPO) characterized by girdles of [100] and [001] axes sub-parallel to the shear plane and a cluster of [010] axes sub-normal to the shear plane, namely the AG-type fabric. Based on experiments of two-phase aggregates of olivine + basalt, a two-phase flow theory and a CPO formation model were established to explain these microstructures. Here, we investigate the microstructure in a two-phase aggregate with supercritical CO2 as the fluid phase and examine the theory and model, to evaluate differences in rheological properties due to the presence of CO2 or basaltic melt. We conducted high-temperature and high-pressure shear deformed experiments at 1 GPa and 1100 °C in a Griggs-type apparatus on samples made of olivine + dolomite, which decomposed into carbonate melt and CO2 at experimental conditions. After deformation, CO2 segregation and an AG-type fabric were observed in these CO2-bearing samples, similar to basaltic melt-bearing samples. An SPO-induce CPO model was used to explain to the formation of the fabric. Our results suggest that the influences of CO2 as a fluid phase on the microstructure of a two-phase olivine aggregate is similar to that of basaltic melt and can be explained by the CPO formation model for the solid-fluid system.

Detecting the flowering stage of tea chrysanthemum is a key mechanism of the selective chrysanthemum harvesting robot. However, under complex, unstructured scenarios, such as illumination variation, occlusion, and overlapping, detecting tea chrysanthemum at a specific flowering stage is a real challenge. This paper proposes a highly fused, lightweight detection model named the Fusion-YOLO (F-YOLO) model. First, cutout and mosaic input components are equipped, with which the fusion module can better understand the features of the chrysanthemum through slicing. In the backbone component, the Cross-Stage Partial DenseNet (CSPDenseNet) network is used as the main network, and feature fusion modules are added to maximize the gradient flow difference. Next, in the neck component, the Cross-Stage Partial ResNeXt (CSPResNeXt) network is taken as the main network to truncate the redundant gradient flow. Finally, in the head component, the multi-scale fusion network is adopted to aggregate the parameters of two different detection layers from different backbone layers. The results show that the F-YOLO model is superior to state-of-the-art technologies in terms of object detection, that this method can be deployed on a single mobile GPU, and that it will be one of key technologies to build a selective chrysanthemum harvesting robot system in the future.

Background:β-adrenoceptors play a crucial regulatory role in blood vessel endothelial cells. Isoprenaline (ISO, a β-adrenergic agonist) has been reported to promote angiogenesis through upregulation of vascular endothelial growth factor (VEGF) expression; however, the underlying mechanism remains to be investigated. It is widely accepted that certain noncoding RNAs, including microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), can regulate endothelial cell behavior, including their involvement in angiogenesis. Therefore, we aimed to investigate whether noncoding RNAs participate in ISO-mediated angiogenesis using human umbilical vein endothelial cells (HUVECs). Methods: We evaluated VEGF-A messenger RNA (mRNA) and protein levels in ISO-treated HUVECs by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. To establish whether noncoding RNAs are associated with ISO-mediated angiogenesis, we measured expression of the miRNAs miR-210, miR-21, and miR-1, as well as that of the lncRNAs growth arrest-specific transcript 5 (GAS5), maternally expressed 3 (MEG3), and metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in HUVECs exposed to ISO. Furthermore, to ascertain its importance in ISO-mediated angiogenesis, we constructed the HUVECs with overexpressing miR-210 and detected the subsequent expression of VEGF-A and noncoding RNAs. All statistical analyses were performed using SPSS 16.0 software. Intergroup comparisons were carried out by one-way analysis of variance. Results: VEGF-A mRNA levels were elevated in the ISO group (1.57 ± 0.09) compared to those in the control group (P < 0.01). Moreover, concentrations of VEGF-A in culture supernatants significantly differed between the control (113.00 ± 19.21 pg/ml) and ISO groups (287.00 ± 20.27 pg/ml; P< 0.01). Expression of miR-1, miR-21, and miR-210 was higher (3.89 ± 0.44, 2.87 ± 087, and 3.33 ± 1.31, respectively) in ISO-treated cells than that in controls (P < 0.01), whereas that of GAS5 and MEG3 (0.22 ± 0.10 and 0.58 ± 0.16, respectively) was lower as a result of ISO administration (P < 0.05). There was no significant difference in the expression of MALAT1 between the groups. Interestingly, miR-210 overexpression heightened the levels of VEGF-A and miR-21 (5.87 ± 1.24 and 2.74 ± 1.15, respectively; P< 0.01) and reduced those of GAS5 and MEG3 (0.19 ± 0.01 and 0.09 ± 0.05, respectively; P< 0.01). Conclusions: ISO-mediated angiogenesis was associated with altered expression of miR-210, miR-21, and the lncRNAs GAS5 and MEG3. The effects of miR-210 on the expression of VEGF-A and noncoding RNAs were similar to those of ISO, indicating that it might play an important role in ISO-mediated angiogenesis.

In the current study, the effect of hydrogen atoms on the intergranular failure of α-iron is examined by a molecular dynamics (MD) simulation. The effect of hydrogen embrittlement on the grain boundary (GB) is investigated by diffusing hydrogen atoms into the grain boundaries using a bicrystal body-centered cubic (BCC) model and then deforming the model with a uniaxial tension. The Debye Waller factors are applied to illustrate the volume change of GBs, and the simulation results suggest that the trapped hydrogen atoms in GBs can therefore increase the excess volume of GBs, thus enhancing intergranular failure. When a constant displacement loading is applied to the bicrystal model, the increased strain energy can barely be released via dislocation emission when H is present. The hydrogen pinning effect occurs in the current dislocation slip system, {112}. The hydrogen atoms facilitate cracking via a decrease of the free surface energy and enhance the phase transition via an increase in the local pressure. Hence, the failure mechanism is prone to intergranular failure so as to release excessive pressure and energy near GBs. This study provides a mechanistic framework of intergranular failure, and a theoretical model is then developed to predict the intergranular cracking rate.

We investigated the microstructures of five ice core samples from the Styx Glacier, northern Victoria Land, Antarctica. Evidence of dynamic recrystallization was found in all samples: those at 50 m mainly by polygonization, and those at 170 m, largely by grain boundary migration. Crystallographic preferred orientations of all analyzed samples (view from the surface) typically showed a single cluster of c-axes normal to the surface. A girdle intersecting the single cluster occurs at 140–170 m with a tight cluster of a-axes normal to the girdle. We interpret the change of crystallographic preferred orientations (CPOs) at 140 m, as the result of horizontal extension. Based on the data obtained from the ground penetrating radar, the underlying bedrock topography of a nunatak could have generated the extensional stress regime in the study area. The results imply changeable stress regimes that may occur during burial as a result of external kinematic controls, such as an appearance of a small peak in the bedrock.

Bacterial lipoproteins are a set of membrane proteins with various functions; many of which are virulence factors of pathogenic bacteria. In the present study, we investigated the role of an outer membrane lipoprotein Lip40 in the pathogenesis of Actinobacillus pleuropneumoniae. A mutant strain (Δlip40) lacking Lip40 and a complemented strain (CΔlip40) were constructed. Δlip40 exhibited reduced adherence to the St. Jude porcine lung cells. The ability of the Δlip40 mutant to colonize the mouse lung tissues was significantly impaired compared to that of the wild type and complementation strains. Furthermore, an infection assay revealed that pigs infected with Δlip40 showed fewer clinical signs and lung lesions, indicating that Lip40 contributed to the development of porcine pleuropneumonia. Collectively, our data suggest that Lip40 is involved in the virulence of A. pleuropneumoniae.

Phase change memory is known as the most promising candidate for the next generation nonvolatile memory technology. In this paper, the microstructural changes of Ge2Sb2Te5 film, which is the most common choice of phase change memory material, has been carefully studied by the combination of several characterization techniques. The combination of resistance measurements, X-ray diffraction, Raman spectroscopy and X-ray reflectivity allows us to simultaneously extract the characteristics of microstructural changes during crystallization process. The existence of surface/interface Ge2Sb2Te5 layer has been proposed here based on X-ray reflectivity measurements. Although the total film thickness decreases, as a result of the phase transition from amorphous to metastable crystalline cubic and then to the stable hexagonal phase, the surface/interface thickness increases after crystallization. Moreover, the increase of average grain size, density and surface roughness has been confirmed during thermal annealing process.

Hypertension is a significant global public health problem and recognized as an important risk factor for cardiovascular diseases. This study was designed to assess the current prevalence of hypertension and to explore risk factors associated with hypertension by urban and rural status to guide the prevention and control of hypertension in Jilin province.A multi-stage stratified random cluster sampling method was used to obtain data on hypertension, which was investigated by physical examination and face-to-face questionnaire in July 2014-December 2015. Sample data were analyzed by complex weighted statistical analysis to estimate blood pressure levels and prevalence of hypertension in the province. Multivariable logistic regression analysis was used to identify factors influencing hypertension rates.The prevalence of hypertension was significantly higher in rural areas than urban areas (25.93% versus 22.73%, respectively). The rates of hypertension known (46.7% versus 38.1%, respectively), control (13.7% versus 5.0%, respectively), and controlled among treated subjects (38.3% versus 17.5%, respectively) were higher in urban areas than in rural areas (all p < 0.001), while the treatment rate was not statistically significantly different between urban and rural areas (35.9% versus 28.4%, respectively). After adjusting for demographic covariates, hypertension prevalence in rural areas was still significantly greater than in urban areas (adjusted OR = 1.22; 95%CI: 1.10, 1.36; p < 0.001). Common risk factors for hypertension among urban and rural residents included older age; male; married; employed; less education; overweight/obese; greater abdominal waist circumference; family history of hypertension, stroke, or coronary heart disease; current smoker; alcohol consumption; higher visceral adiposity index; and higher body fat percentage.This study identified an increased risk for hypertension in rural regions of Jilin province, suggesting that rural hypertension screening and treatment guidelines should receive greater attention.

The microstructure and electro-magnetic properties of simultaneous Ba doping for Sr and Co doping for Ru in SrRuO3 have been carefully investigated. All the doped samples exhibit the same orthorhombic Pnma structure as the pure bulk SrRuO3, although introducing some local structural modifications due to the ionic radius mismatch at Sr/Ru-site. A maximum of 2.2% negative magnetoresistance and much suppressed Curie temperature are found for the most insulating Ba and Co co-doped Sr0.88Ba0.12Ru0.88Co0.12O3 sample. The substitution induced A-site disorder, B-site ion dilution, structural distortion and the intrinsic nature of cobalt ion etc. are jointly considered to explain the observed interesting phenomena.